Heg1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Heg1
Entrez Gene ID	77446
Full Name	heart development protein with EGF-like domains 1
Synonyms	4632417D23Rik,5530401I02Rik,9530025L16Rik,BC038614,Gm629
General protein information	Preferred Names heart development protein with EGF-like domains 1 Names protein HEG homolog 1 HEG homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006522717.1	XP_006522780.1	protein HEG homolog 1 isoform X3
NM_175256.5	NP_780465.4	protein HEG homolog 1 precursor
XM_006522718.2	XP_006522781.1	protein HEG homolog 1 isoform X4
XM_017317159.1	XP_017172648.1	protein HEG homolog 1 isoform X5
XM_006522716.1	XP_006522779.1	protein HEG homolog 1 isoform X2
XM_006522715.3	XP_006522778.1	protein HEG homolog 1 isoform X1
XM_006522716.2	XP_006522779.1	protein HEG homolog 1 isoform X2
XM_006522717.2	XP_006522780.1	protein HEG homolog 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	HEG1	NP_065784.1
	HEG1	XP_002802724.1
Bos taurus (cattle)	HEG1	XP_002684860.1
Mus musculus (house mouse)	Heg1	NP_780465.4
Rattus norvegicus (Norway rat)	Heg1	XP_006248505.1
Pan troglodytes (chimpanzee)	HEG1	XP_516708.4
Canis lupus familiaris (dog)	HEG1	XP_005639641.1

Gene Ontology
Bibliography

Related articles in PubMed

Heart of glass anchors Rasip1 at endothelial cell-cell junctions to support vascular integrity.
de Kreuk BJ, Gingras AR, Knight JD, Liu JJ, Gingras AC, Ginsberg MH
eLife5e11394(2016 Jan)

Dynamic regulation of the cerebral cavernous malformation pathway controls vascular stability and growth.
Zheng X, Xu C, Smith AO, Stratman AN, Zou Z, Kleaveland B, Yuan L, Didiku C, Sen A, Liu X, Skuli N, Zaslavsky A, Chen M, Cheng L, Davis GE, Kahn ML
Developmental cell23(2)342-55(2012 Aug)

Gene expression analysis of the embryonic subplate.
Oeschger FM, Wang WZ, Lee S, Garc?a-Moreno F, Goffinet AM, Arbon?s ML, Rakic S, Moln?r Z
Cerebral cortex (New York, N.Y. : 1991)22(6)1343-59(2012 Jun)

Regulation of cardiovascular development and integrity by the heart of glass-cerebral cavernous malformation protein pathway.
Kleaveland B, Zheng X, Liu JJ, Blum Y, Tung JJ, Zou Z, Sweeney SM, Chen M, Guo L, Lu MM, Zhou D, Kitajewski J, Affolter M, Ginsberg MH, Kahn ML
Nature medicine15(2)169-76(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Defects in the hearts of zebrafish lacking heg or ccm2, in the aortas of early mouse embryos lacking CCM2 and in the lymphatic vessels of neonatal mice lacking HEG1 were associated with abnormal endothelial cell junctions like those observed in human CCMs
Title: Regulation of cardiovascular development and integrity by the heart of glass-cerebral cavernous malformation protein pathway.

The following Heg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Heg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu79412	XM_017317159.1 Latest version!	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OMu52518	XM_006522716.2 Latest version!	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu52519	XM_006522717.2 Latest version!	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu05083	NM_175256.5 Latest version!	Mus musculus heart development protein with EGF-like domains 1 (Heg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$636.30 $909.00
OMu52517	XM_006522715.3 Latest version!	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu52520	XM_006522718.2 Latest version!	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OMu52518	XM_006522716.1	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu52519	XM_006522717.1	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Heg1 ( XM_017317159.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172648.1 Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu79412
Clone ID Related Accession (Same CDS sequence)	XM_017317159.1
Accession Version	XM_017317159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein HEG homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTG ACAGCTCTGA TCTGGCAGAC AGCACTTCGG GGGCTCGCAC ACCTCACACG 
TCTGCAATGT CCACCCGGAG CGGGGAGCGC ACGCTGCGGT CCCTTGACCT CTCGAGTGCA 
GCAACCCGCC CTGCGCGCCC CACTCCACGA GGGAACGTGA CAGAGCACGC TGGCCTGCTG 
TCCGGGGCAC CAACTCTCGG AGTCACCGGA CTTAGCTACA CTCGAGAACA TGGCTCAGAC 
GCTGGGCAGA GGACTTCCAG TGACCACACA GACCACGGCT ATGTTCCGTC TACTTTCACC 
AAAGGAGAAC GGACGCTACT GTCTATTACA GATAACACCT CCTACTCAGA GGCCAGCGAG 
AGCTCAACCT CTTCCGTTAA GATCTCAGAC TCTCCTTCTC AGGCTCAGCC TAAACAGAGT 
AGCATGTCCT CCGACGATGA CGAGCCTGCT CAGTCTTCCA CAGAGTCACC GGTTCTGCAT 
ACGTCCAACC TGCCAACCTA CACGTCCACT GTTAATATGC CAAACACCTT GGTTCTTGAT 
ACCGGTACCA AACCTGTTGA GGACCCATCT GATTCACGGG TGCCTTCCAC TCAACCCTCA 
CCGTCACAAC CCCAGCCATT CTCATCAGCC TTACCATCAA CCAGGTCTCC TGGGTCTACC 
TCTGAGACAA CCACATCCTC ACCATCACCT TCACCCATCT CCTTACTGGT GTCAACTCTT 
GCCCCATATT CTGTCTCACA AACAACCTTC CCACATCCAT CTTCTACCCT AGTCCCTCAC 
AGGCCAAGGG AGCCACGTGT GACTTCAGTC CAAATGTCGA CGGCAATATC AGCCATTGCA 
TTGATCCCCA GCAATCAAAC AGCAAACCCC AAGAACCAGA GTACCCCACA ACAAGAGAAA 
CCCATCACAG AAGCTAAGTC ACCAAGCTTG GTGTCTCCGC CCACAGACTC TACCAAAGCA 
GTAACAGTGA GCCTTCCTCC AGGGGCCCCT TGGTCCCCAG CCTTAACAGG GTTCTCCACG 
GGGCCAGCCC TTCCAGCCAC AAGCACCAGT TTAGCCCAGA TGTCTCCAGC TTTGACATCT 
GCCATGCCCC AGACGACTCA CTCTCCTGTG ACCAGTCCCA GTACTCTGTC ACATGTAGAA 
GCTTTGACTT CAGGTGCTGT AGTTGTGCAC ACTACCCCGA AGAAACCACA TCTCCCAACC 
AATCCTGAAA TCCTAGTGCC ACACATCTCA ACTGAGGGGG CCATCACCAC AGAAGGGAAC 
CGGGAGCATA CGGATCCTAC CACTCAGCCA ATCCCTCTGA CCACTTCAAC CACGTCAGCA 
GGAGAAAGGA CCACAGAGCT GGGCCGTGCG GAAGAGTCCA GTCCATCCCA TTTTCTTACA 
CCATCTTCAC CTCAAACCAC AGATGTTTCT ACAGCTGAAA TGTTGACTTC CAGATATATC 
ACCTTTGCTG CTCAGAGCAC CTCACAGTCA CCAACAGCCT TGCCACCTCT AACCCCAGTC 
AACAGCTGTA CCGTGAACCC TTGTCTTCAT GATGGCAAAT GCATTGTGGA CCTCACTGGC 
CGTGGGTATC GATGTGTGTG CCCGCCTGCC TGGCAGGGGG AGAACTGCAG TGTGGATGTG 
AACGAATGTC TCTCCAGCCC CTGTCCTCCC CTAGCCACGT GCAACAATAC TCAGGGATCG 
TTCACCTGCA GATGCCCAGT CGGGTATCAG TTGGAGAAAG GAATATGCAA TCTGGTTAGA 
ACCTTTGTGA CCGAGTTTAA GTTAAAGAAG ACTTTTCTCA ATACTACTGC AGAAAACCAT 
TCCAACACTC AAGAGCTTGA AAACGAGATC GCTCAAACAC TAAACGTGTG TTTTTCAACA 
TTGCCCGGTT ATATCCGAAC TACAGCCCAT GTGTCTAGGG AGCCCAGTAC AGTTTTCATC 
TCACTGAAAA CCACCTTTGC CCTGGCCTCC AATGTGACAC TGTTTGACCT GGCAGACAGG 
ATCCAGAAAT ATGTCAACTC CTGCAGGTCC TCTGCTGAAG TCTGCCAGCT CTTGGGGTCT 
CAGAGGCGGG TCTTTCGAGC GGGCAGCTTG TGTAAGCGGA AGAGTCCAGA ATGTGACAAA 
GAGACCTCCA TCTGCACCGA CCTGGATGGT GTCGCGCTCT GTCAGTGCAA GTCTGGCTAC 
TTCCAGTTCA ACAAGATGGA TCACTCTTGC CGAGCATGTG AAGATGGATA TAGGCTTGAA 
AATGAAACCT GTATGAGTTG CCCATTTGGC CTCGGTGGAC TCAACTGTGG AAACCCCTAT 
CAGCTCATCA CTGTGGTGAT CGCAGCCGCA GGAGGTGGCC TCTTGCTCAT CCTGGGCGTG 
GCACTCATTG TTACCTGTTG CAGAAAGAGT AAAAATGACA TAAGTAAACT CATCTTCAAA 
AGTGGGGACT TCCAAATGTC CCCATACACC GATGTCCCCA AGAATCCTCG CTCGCAAGAG 
TGGGGCCGAG AAGCCATTGA AATGCACGAG AACGGAAGCA CCAAAAACCT CCTGCAGATG 
ACAGACGTGT ACTACTCGCC CACAAACGTA AGGAACCCTG AACTTGAACG CAACGGACTG 
TACCCAGCCT ACACTGGACT GCCCGGATCG AGACACTCTT GCATCTTCCC GGGACAGTAT 
AACCCATCTT TCATCAGCGA CGAGAGCAGG AGGAGAGACT ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172648.1
CDS	269..2956
Translation	MLSDSSDLADSTSGARTPHTSAMSTRSGERTLRSLDLSSAATRPARPTPRGNVTEHAGLLSGAPTLGVTGLSYTREHGSDAGQRTSSDHTDHGYVPSTFTKGERTLLSITDNTSYSEASESSTSSVKISDSPSQAQPKQSSMSSDDDEPAQSSTESPVLHTSNLPTYTSTVNMPNTLVLDTGTKPVEDPSDSRVPSTQPSPSQPQPFSSALPSTRSPGSTSETTTSSPSPSPISLLVSTLAPYSVSQTTFPHPSSTLVPHRPREPRVTSVQMSTAISAIALIPSNQTANPKNQSTPQQEKPITEAKSPSLVSPPTDSTKAVTVSLPPGAPWSPALTGFSTGPALPATSTSLAQMSPALTSAMPQTTHSPVTSPSTLSHVEALTSGAVVVHTTPKKPHLPTNPEILVPHISTEGAITTEGNREHTDPTTQPIPLTTSTTSAGERTTELGRAEESSPSHFLTPSSPQTTDVSTAEMLTSRYITFAAQSTSQSPTALPPLTPVNSCTVNPCLHDGKCIVDLTGRGYRCVCPPAWQGENCSVDVNECLSSPCPPLATCNNTQGSFTCRCPVGYQLEKGICNLVRTFVTEFKLKKTFLNTTAENHSNTQELENEIAQTLNVCFSTLPGYIRTTAHVSREPSTVFISLKTTFALASNVTLFDLADRIQKYVNSCRSSAEVCQLLGSQRRVFRAGSLCKRKSPECDKETSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGVALIVTCCRKSKNDISKLIFKSGDFQMSPYTDVPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTNVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

Target ORF information:

RefSeq Version	XM_017317159.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317159.1

ORF Insert Sequence:

ATGCTGAGTG ACAGCTCTGA TCTGGCAGAC AGCACTTCGG GGGCTCGCAC ACCTCACACG 
TCTGCAATGT CCACCCGGAG CGGGGAGCGC ACGCTGCGGT CCCTTGACCT CTCGAGTGCA 
GCAACCCGCC CTGCGCGCCC CACTCCACGA GGGAACGTGA CAGAGCACGC TGGCCTGCTG 
TCCGGGGCAC CAACTCTCGG AGTCACCGGA CTTAGCTACA CTCGAGAACA TGGCTCAGAC 
GCTGGGCAGA GGACTTCCAG TGACCACACA GACCACGGCT ATGTTCCGTC TACTTTCACC 
AAAGGAGAAC GGACGCTACT GTCTATTACA GATAACACCT CCTACTCAGA GGCCAGCGAG 
AGCTCAACCT CTTCCGTTAA GATCTCAGAC TCTCCTTCTC AGGCTCAGCC TAAACAGAGT 
AGCATGTCCT CCGACGATGA CGAGCCTGCT CAGTCTTCCA CAGAGTCACC GGTTCTGCAT 
ACGTCCAACC TGCCAACCTA CACGTCCACT GTTAATATGC CAAACACCTT GGTTCTTGAT 
ACCGGTACCA AACCTGTTGA GGACCCATCT GATTCACGGG TGCCTTCCAC TCAACCCTCA 
CCGTCACAAC CCCAGCCATT CTCATCAGCC TTACCATCAA CCAGGTCTCC TGGGTCTACC 
TCTGAGACAA CCACATCCTC ACCATCACCT TCACCCATCT CCTTACTGGT GTCAACTCTT 
GCCCCATATT CTGTCTCACA AACAACCTTC CCACATCCAT CTTCTACCCT AGTCCCTCAC 
AGGCCAAGGG AGCCACGTGT GACTTCAGTC CAAATGTCGA CGGCAATATC AGCCATTGCA 
TTGATCCCCA GCAATCAAAC AGCAAACCCC AAGAACCAGA GTACCCCACA ACAAGAGAAA 
CCCATCACAG AAGCTAAGTC ACCAAGCTTG GTGTCTCCGC CCACAGACTC TACCAAAGCA 
GTAACAGTGA GCCTTCCTCC AGGGGCCCCT TGGTCCCCAG CCTTAACAGG GTTCTCCACG 
GGGCCAGCCC TTCCAGCCAC AAGCACCAGT TTAGCCCAGA TGTCTCCAGC TTTGACATCT 
GCCATGCCCC AGACGACTCA CTCTCCTGTG ACCAGTCCCA GTACTCTGTC ACATGTAGAA 
GCTTTGACTT CAGGTGCTGT AGTTGTGCAC ACTACCCCGA AGAAACCACA TCTCCCAACC 
AATCCTGAAA TCCTAGTGCC ACACATCTCA ACTGAGGGGG CCATCACCAC AGAAGGGAAC 
CGGGAGCATA CGGATCCTAC CACTCAGCCA ATCCCTCTGA CCACTTCAAC CACGTCAGCA 
GGAGAAAGGA CCACAGAGCT GGGCCGTGCG GAAGAGTCCA GTCCATCCCA TTTTCTTACA 
CCATCTTCAC CTCAAACCAC AGATGTTTCT ACAGCTGAAA TGTTGACTTC CAGATATATC 
ACCTTTGCTG CTCAGAGCAC CTCACAGTCA CCAACAGCCT TGCCACCTCT AACCCCAGTC 
AACAGCTGTA CCGTGAACCC TTGTCTTCAT GATGGCAAAT GCATTGTGGA CCTCACTGGC 
CGTGGGTATC GATGTGTGTG CCCGCCTGCC TGGCAGGGGG AGAACTGCAG TGTGGATGTG 
AACGAATGTC TCTCCAGCCC CTGTCCTCCC CTAGCCACGT GCAACAATAC TCAGGGATCG 
TTCACCTGCA GATGCCCAGT CGGGTATCAG TTGGAGAAAG GAATATGCAA TCTGGTTAGA 
ACCTTTGTGA CCGAGTTTAA GTTAAAGAAG ACTTTTCTCA ATACTACTGC AGAAAACCAT 
TCCAACACTC AAGAGCTTGA AAACGAGATC GCTCAAACAC TAAACGTGTG TTTTTCAACA 
TTGCCCGGTT ATATCCGAAC TACAGCCCAT GTGTCTAGGG AGCCCAGTAC AGTTTTCATC 
TCACTGAAAA CCACCTTTGC CCTGGCCTCC AATGTGACAC TGTTTGACCT GGCAGACAGG 
ATCCAGAAAT ATGTCAACTC CTGCAGGTCC TCTGCTGAAG TCTGCCAGCT CTTGGGGTCT 
CAGAGGCGGG TCTTTCGAGC GGGCAGCTTG TGTAAGCGGA AGAGTCCAGA ATGTGACAAA 
GAGACCTCCA TCTGCACCGA CCTGGATGGT GTCGCGCTCT GTCAGTGCAA GTCTGGCTAC 
TTCCAGTTCA ACAAGATGGA TCACTCTTGC CGAGCATGTG AAGATGGATA TAGGCTTGAA 
AATGAAACCT GTATGAGTTG CCCATTTGGC CTCGGTGGAC TCAACTGTGG AAACCCCTAT 
CAGCTCATCA CTGTGGTGAT CGCAGCCGCA GGAGGTGGCC TCTTGCTCAT CCTGGGCGTG 
GCACTCATTG TTACCTGTTG CAGAAAGAGT AAAAATGACA TAAGTAAACT CATCTTCAAA 
AGTGGGGACT TCCAAATGTC CCCATACACC GATGTCCCCA AGAATCCTCG CTCGCAAGAG 
TGGGGCCGAG AAGCCATTGA AATGCACGAG AACGGAAGCA CCAAAAACCT CCTGCAGATG 
ACAGACGTGT ACTACTCGCC CACAAACGTA AGGAACCCTG AACTTGAACG CAACGGACTG 
TACCCAGCCT ACACTGGACT GCCCGGATCG AGACACTCTT GCATCTTCCC GGGACAGTAT 
AACCCATCTT TCATCAGCGA CGAGAGCAGG AGGAGAGACT ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heg1 ( XM_006522716.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522779.1 Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu52518
Clone ID Related Accession (Same CDS sequence)	XM_006522716.1 , XM_006522716.2
Accession Version	XM_006522716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein HEG homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCTTGG AGAGCCTTCC TGAGTCACCC AGCAGTTCCA GGTCACAAAG AAGAATCACA 
CCTTCTCAAA CAGAGAGTGG GACATCCCTA GGCTTCCTGG AGAGGACGAG GGAGCTCCCA 
GAAGAAGGGA CTGTGCACAC GCAAGTGGCT GGCACCTGGG TGTCCCGCCA AGCCTCCCAC 
CCTGCTCTGG AACCTGGGGA GCCAACTGTG CTCTCCCAGA AGAGAAATTC ATCCGGCCAG 
GAACACTCCG GGCCACCATT CTCCTGGAGT CAGAGTCACC CTCCTCCCTC AGACCACCCA 
TCGTCTTCTG GAAGTATTAA GAATGGTAAT AATTTCACCG CTCTCCAAAA CCCATCAGTC 
ACCCAGACAA AGAGCATGCT TATCACTGAC ACATACACTA ATGGCGTCCC AAGAACTCTG 
CGGTCTCTGC CCGTTGGTGT GGACCCTGCA GATGAGACAG AGGGTTTCCC TGAGCACTCC 
AGACTTGGTA TAACTTCAAT GTCAGTCCGC TCTTCACCCT CTGTGAAGGA CTCAAGGACA 
AACAGTGGAC TAACTGAGCA TCTGGGAGAT GGAGAAGGCA CTGAGCTGTC TACAGAAAAT 
GGATATGGAC TTCCATCTAT ACACTGGCAA AGTGATGCCC CCTCCTTCGG AGGACGTCAG 
CTTGCCAGCA GCTCAGAAGC GGGCGATGGA AGGGCCATGC CTCTGACTGA GGCTGTGTTT 
AGATCAGACC CCTCCATTGG TGGTGGAGAG AGCACCGGAC GATGGATCCT CACCAAGAAG 
AAAACATCTA CAGATGCGGC AGAAAGCTCA GCTTTGCATC CAGAAGCTGG GGGTGCTGGG 
GGCTTGACTC AGTCCTCACA CGCTGCACAA CAGCCCCGAG GTGGCGGCGA AGACTCAGGG 
ATGGGTGGGA GGAGTTACGC AGAGTCTTCA TCTTCTTCTT CATCTACATC TTCTTCCGAA 
AGTCTGGATT CCTCAGCACC TCTCAGAGAG CATTCTCCTC CGGAAGATGG TGCAATGCTG 
AGTGACAGCT CTGATCTGGC AGACAGCACT TCGGGGGCTC GCACACCTCA CACGTCTGCA 
ATGTCCACCC GGAGCGGGGA GCGCACGCTG CGGTCCCTTG ACCTCTCGAG TGCAGCAACC 
CGCCCTGCGC GCCCCACTCC ACGAGGGAAC GTGACAGAGC ACGCTGGCCT GCTGTCCGGG 
GCACCAACTC TCGGAGTCAC CGGACTTAGC TACACTCGAG AACATGGCTC AGACGCTGGG 
CAGAGGACTT CCAGTGACCA CACAGACCAC GGCTATGTTC CGTCTACTTT CACCAAAGGA 
GAACGGACGC TACTGTCTAT TACAGATAAC ACCTCCTACT CAGAGGCCAG CGAGAGCTCA 
ACCTCTTCCG TTAAGATCTC AGACTCTCCT TCTCAGGCTC AGCCTAAACA GAGTAGCATG 
TCCTCCGACG ATGACGAGCC TGCTCAGTCT TCCACAGAGT CACCGGTTCT GCATACGTCC 
AACCTGCCAA CCTACACGTC CACTGTTAAT ATGCCAAACA CCTTGGTTCT TGATACCGGT 
ACCAAACCTG TTGAGGACCC ATCTGATTCA CGGGTGCCTT CCACTCAACC CTCACCGTCA 
CAACCCCAGC CATTCTCATC AGCCTTACCA TCAACCAGGT CTCCTGGGTC TACCTCTGAG 
ACAACCACAT CCTCACCATC ACCTTCACCC ATCTCCTTAC TGGTGTCAAC TCTTGCCCCA 
TATTCTGTCT CACAAACAAC CTTCCCACAT CCATCTTCTA CCCTAGTCCC TCACAGGCCA 
AGGGAGCCAC GTGTGACTTC AGTCCAAATG TCGACGGCAA TATCAGCCAT TGCATTGATC 
CCCAGCAATC AAACAGCAAA CCCCAAGAAC CAGAGTACCC CACAACAAGA GAAACCCATC 
ACAGAAGCTA AGTCACCAAG CTTGGTGTCT CCGCCCACAG ACTCTACCAA AGCAGTAACA 
GTGAGCCTTC CTCCAGGGGC CCCTTGGTCC CCAGCCTTAA CAGGGTTCTC CACGGGGCCA 
GCCCTTCCAG CCACAAGCAC CAGTTTAGCC CAGATGTCTC CAGCTTTGAC ATCTGCCATG 
CCCCAGACGA CTCACTCTCC TGTGACCAGT CCCAGTACTC TGTCACATGT AGAAGCTTTG 
ACTTCAGGTG CTGTAGTTGT GCACACTACC CCGAAGAAAC CACATCTCCC AACCAATCCT 
GAAATCCTAG TGCCACACAT CTCAACTGAG GGGGCCATCA CCACAGAAGG GAACCGGGAG 
CATACGGATC CTACCACTCA GCCAATCCCT CTGACCACTT CAACCACGTC AGCAGGAGAA 
AGGACCACAG AGCTGGGCCG TGCGGAAGAG TCCAGTCCAT CCCATTTTCT TACACCATCT 
TCACCTCAAA CCACAGATGT TTCTACAGCT GAAATGTTGA CTTCCAGATA TATCACCTTT 
GCTGCTCAGA GCACCTCACA GTCACCAACA GCCTTGCCAC CTCTAACCCC AGTCAACAGC 
TGTACCGTGA ACCCTTGTCT TCATGATGGC AAATGCATTG TGGACCTCAC TGGCCGTGGG 
TATCGATGTG TGTGCCCGCC TGCCTGGCAG GGGGAGAACT GCAGTGTGGA TGTGAACGAA 
TGTCTCTCCA GCCCCTGTCC TCCCCTAGCC ACGTGCAACA ATACTCAGGG ATCGTTCACC 
TGCAGATGCC CAGTCGGGTA TCAGTTGGAG AAAGGAATAT GCAATCTGGT TAGAACCTTT 
GTGACCGAGT TTAAGTTAAA GAAGACTTTT CTCAATACTA CTGCAGAAAA CCATTCCAAC 
ACTCAAGAGC TTGAAAACGA GATCGCTCAA ACACTAAACG TGTGTTTTTC AACATTGCCC 
GGTTATATCC GAACTACAGC CCATGTGTCT AGGGAGCCCA GTACAGTTTT CATCTCACTG 
AAAACCACCT TTGCCCTGGC CTCCAATGTG ACACTGTTTG ACCTGGCAGA CAGGATCCAG 
AAATATGTCA ACTCCTGCAG GTCCTCTGCT GAAGTCTGCC AGCTCTTGGG GTCTCAGAGG 
CGGGTCTTTC GAGCGGGCAG CTTGTGTAAG CGGAAGAGTC CAGAATGTGA CAAAGAGACC 
TCCATCTGCA CCGACCTGGA TGGTGTCGCG CTCTGTCAGT GCAAGTCTGG CTACTTCCAG 
TTCAACAAGA TGGATCACTC TTGCCGAGCA TGTGAAGATG GATATAGGCT TGAAAATGAA 
ACCTGTATGA GTTGCCCATT TGGCCTCGGT GGACTCAACT GTGGAAACCC CTATCAGCTC 
ATCACTGTGG TGATCGCAGC CGCAGGAGGT GGCCTCTTGC TCATCCTGGG CGTGGCACTC 
ATTGTTACCT GTTGCAGAAA GAGTAAAAAT GACATAAGTA AACTCATCTT CAAAAGTGGG 
GACTTCCAAA TGTCCCCATA CACCGATGTC CCCAAGAATC CTCGCTCGCA AGAGTGGGGC 
CGAGAAGCCA TTGAAATGCA CGAGAACGGA AGCACCAAAA ACCTCCTGCA GATGACAGAC 
GTGTACTACT CGCCCACAAA CGTAAGGAAC CCTGAACTTG AACGCAACGG ACTGTACCCA 
GCCTACACTG GACTGCCCGG ATCGAGACAC TCTTGCATCT TCCCGGGACA GTATAACCCA 
TCTTTCATCA GCGACGAGAG CAGGAGGAGA GACTACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522779.1
CDS	229..4170
Translation	MATPRAPRWPPPSLLLLLLLPLLLLPPAAPGARGSLPSPAHRTLLPVAGPLSPPGAGHTAPGPGVATRRGRSGRVPRGVSAALESLPESPSSSRSQRRITPSQTESGTSLGFLERTRELPEEGTVHTQVAGTWVSRQASHPALEPGEPTVLSQKRNSSGQEHSGPPFSWSQSHPPPSDHPSSSGSIKNGNNFTALQNPSVTQTKSMLITDTYTNGVPRTLRSLPVGVDPADETEGFPEHSRLGITSMSVRSSPSVKDSRTNSGLTEHLGDGEGTELSTENGYGLPSIHWQSDAPSFGGRQLASSSEAGDGRAMPLTEAVFRSDPSIGGGESTGRWILTKKKTSTDAAESSALHPEAGGAGGLTQSSHAAQQPRGGGEDSGMGGRSYAESSSSSSSTSSSESLDSSAPLREHSPPEDGAMLSDSSDLADSTSGARTPHTSAMSTRSGERTLRSLDLSSAATRPARPTPRGNVTEHAGLLSGAPTLGVTGLSYTREHGSDAGQRTSSDHTDHGYVPSTFTKGERTLLSITDNTSYSEASESSTSSVKISDSPSQAQPKQSSMSSDDDEPAQSSTESPVLHTSNLPTYTSTVNMPNTLVLDTGTKPVEDPSDSRVPSTQPSPSQPQPFSSALPSTRSPGSTSETTTSSPSPSPISLLVSTLAPYSVSQTTFPHPSSTLVPHRPREPRVTSVQMSTAISAIALIPSNQTANPKNQSTPQQEKPITEAKSPSLVSPPTDSTKAVTVSLPPGAPWSPALTGFSTGPALPATSTSLAQMSPALTSAMPQTTHSPVTSPSTLSHVEALTSGAVVVHTTPKKPHLPTNPEILVPHISTEGAITTEGNREHTDPTTQPIPLTTSTTSAGERTTELGRAEESSPSHFLTPSSPQTTDVSTAEMLTSRYITFAAQSTSQSPTALPPLTPVNSCTVNPCLHDGKCIVDLTGRGYRCVCPPAWQGENCSVDVNECLSSPCPPLATCNNTQGSFTCRCPVGYQLEKGICNLVRTFVTEFKLKKTFLNTTAENHSNTQELENEIAQTLNVCFSTLPGYIRTTAHVSREPSTVFISLKTTFALASNVTLFDLADRIQKYVNSCRSSAEVCQLLGSQRRVFRAGSLCKRKSPECDKETSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGVALIVTCCRKSKNDISKLIFKSGDFQMSPYTDVPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTNVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

Target ORF information:

RefSeq Version	XM_006522716.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522716.2

ORF Insert Sequence:

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCTTGG AGAGCCTTCC TGAGTCACCC AGCAGTTCCA GGTCACAAAG AAGAATCACA 
CCTTCTCAAA CAGAGAGTGG GACATCCCTA GGCTTCCTGG AGAGGACGAG GGAGCTCCCA 
GAAGAAGGGA CTGTGCACAC GCAAGTGGCT GGCACCTGGG TGTCCCGCCA AGCCTCCCAC 
CCTGCTCTGG AACCTGGGGA GCCAACTGTG CTCTCCCAGA AGAGAAATTC ATCCGGCCAG 
GAACACTCCG GGCCACCATT CTCCTGGAGT CAGAGTCACC CTCCTCCCTC AGACCACCCA 
TCGTCTTCTG GAAGTATTAA GAATGGTAAT AATTTCACCG CTCTCCAAAA CCCATCAGTC 
ACCCAGACAA AGAGCATGCT TATCACTGAC ACATACACTA ATGGCGTCCC AAGAACTCTG 
CGGTCTCTGC CCGTTGGTGT GGACCCTGCA GATGAGACAG AGGGTTTCCC TGAGCACTCC 
AGACTTGGTA TAACTTCAAT GTCAGTCCGC TCTTCACCCT CTGTGAAGGA CTCAAGGACA 
AACAGTGGAC TAACTGAGCA TCTGGGAGAT GGAGAAGGCA CTGAGCTGTC TACAGAAAAT 
GGATATGGAC TTCCATCTAT ACACTGGCAA AGTGATGCCC CCTCCTTCGG AGGACGTCAG 
CTTGCCAGCA GCTCAGAAGC GGGCGATGGA AGGGCCATGC CTCTGACTGA GGCTGTGTTT 
AGATCAGACC CCTCCATTGG TGGTGGAGAG AGCACCGGAC GATGGATCCT CACCAAGAAG 
AAAACATCTA CAGATGCGGC AGAAAGCTCA GCTTTGCATC CAGAAGCTGG GGGTGCTGGG 
GGCTTGACTC AGTCCTCACA CGCTGCACAA CAGCCCCGAG GTGGCGGCGA AGACTCAGGG 
ATGGGTGGGA GGAGTTACGC AGAGTCTTCA TCTTCTTCTT CATCTACATC TTCTTCCGAA 
AGTCTGGATT CCTCAGCACC TCTCAGAGAG CATTCTCCTC CGGAAGATGG TGCAATGCTG 
AGTGACAGCT CTGATCTGGC AGACAGCACT TCGGGGGCTC GCACACCTCA CACGTCTGCA 
ATGTCCACCC GGAGCGGGGA GCGCACGCTG CGGTCCCTTG ACCTCTCGAG TGCAGCAACC 
CGCCCTGCGC GCCCCACTCC ACGAGGGAAC GTGACAGAGC ACGCTGGCCT GCTGTCCGGG 
GCACCAACTC TCGGAGTCAC CGGACTTAGC TACACTCGAG AACATGGCTC AGACGCTGGG 
CAGAGGACTT CCAGTGACCA CACAGACCAC GGCTATGTTC CGTCTACTTT CACCAAAGGA 
GAACGGACGC TACTGTCTAT TACAGATAAC ACCTCCTACT CAGAGGCCAG CGAGAGCTCA 
ACCTCTTCCG TTAAGATCTC AGACTCTCCT TCTCAGGCTC AGCCTAAACA GAGTAGCATG 
TCCTCCGACG ATGACGAGCC TGCTCAGTCT TCCACAGAGT CACCGGTTCT GCATACGTCC 
AACCTGCCAA CCTACACGTC CACTGTTAAT ATGCCAAACA CCTTGGTTCT TGATACCGGT 
ACCAAACCTG TTGAGGACCC ATCTGATTCA CGGGTGCCTT CCACTCAACC CTCACCGTCA 
CAACCCCAGC CATTCTCATC AGCCTTACCA TCAACCAGGT CTCCTGGGTC TACCTCTGAG 
ACAACCACAT CCTCACCATC ACCTTCACCC ATCTCCTTAC TGGTGTCAAC TCTTGCCCCA 
TATTCTGTCT CACAAACAAC CTTCCCACAT CCATCTTCTA CCCTAGTCCC TCACAGGCCA 
AGGGAGCCAC GTGTGACTTC AGTCCAAATG TCGACGGCAA TATCAGCCAT TGCATTGATC 
CCCAGCAATC AAACAGCAAA CCCCAAGAAC CAGAGTACCC CACAACAAGA GAAACCCATC 
ACAGAAGCTA AGTCACCAAG CTTGGTGTCT CCGCCCACAG ACTCTACCAA AGCAGTAACA 
GTGAGCCTTC CTCCAGGGGC CCCTTGGTCC CCAGCCTTAA CAGGGTTCTC CACGGGGCCA 
GCCCTTCCAG CCACAAGCAC CAGTTTAGCC CAGATGTCTC CAGCTTTGAC ATCTGCCATG 
CCCCAGACGA CTCACTCTCC TGTGACCAGT CCCAGTACTC TGTCACATGT AGAAGCTTTG 
ACTTCAGGTG CTGTAGTTGT GCACACTACC CCGAAGAAAC CACATCTCCC AACCAATCCT 
GAAATCCTAG TGCCACACAT CTCAACTGAG GGGGCCATCA CCACAGAAGG GAACCGGGAG 
CATACGGATC CTACCACTCA GCCAATCCCT CTGACCACTT CAACCACGTC AGCAGGAGAA 
AGGACCACAG AGCTGGGCCG TGCGGAAGAG TCCAGTCCAT CCCATTTTCT TACACCATCT 
TCACCTCAAA CCACAGATGT TTCTACAGCT GAAATGTTGA CTTCCAGATA TATCACCTTT 
GCTGCTCAGA GCACCTCACA GTCACCAACA GCCTTGCCAC CTCTAACCCC AGTCAACAGC 
TGTACCGTGA ACCCTTGTCT TCATGATGGC AAATGCATTG TGGACCTCAC TGGCCGTGGG 
TATCGATGTG TGTGCCCGCC TGCCTGGCAG GGGGAGAACT GCAGTGTGGA TGTGAACGAA 
TGTCTCTCCA GCCCCTGTCC TCCCCTAGCC ACGTGCAACA ATACTCAGGG ATCGTTCACC 
TGCAGATGCC CAGTCGGGTA TCAGTTGGAG AAAGGAATAT GCAATCTGGT TAGAACCTTT 
GTGACCGAGT TTAAGTTAAA GAAGACTTTT CTCAATACTA CTGCAGAAAA CCATTCCAAC 
ACTCAAGAGC TTGAAAACGA GATCGCTCAA ACACTAAACG TGTGTTTTTC AACATTGCCC 
GGTTATATCC GAACTACAGC CCATGTGTCT AGGGAGCCCA GTACAGTTTT CATCTCACTG 
AAAACCACCT TTGCCCTGGC CTCCAATGTG ACACTGTTTG ACCTGGCAGA CAGGATCCAG 
AAATATGTCA ACTCCTGCAG GTCCTCTGCT GAAGTCTGCC AGCTCTTGGG GTCTCAGAGG 
CGGGTCTTTC GAGCGGGCAG CTTGTGTAAG CGGAAGAGTC CAGAATGTGA CAAAGAGACC 
TCCATCTGCA CCGACCTGGA TGGTGTCGCG CTCTGTCAGT GCAAGTCTGG CTACTTCCAG 
TTCAACAAGA TGGATCACTC TTGCCGAGCA TGTGAAGATG GATATAGGCT TGAAAATGAA 
ACCTGTATGA GTTGCCCATT TGGCCTCGGT GGACTCAACT GTGGAAACCC CTATCAGCTC 
ATCACTGTGG TGATCGCAGC CGCAGGAGGT GGCCTCTTGC TCATCCTGGG CGTGGCACTC 
ATTGTTACCT GTTGCAGAAA GAGTAAAAAT GACATAAGTA AACTCATCTT CAAAAGTGGG 
GACTTCCAAA TGTCCCCATA CACCGATGTC CCCAAGAATC CTCGCTCGCA AGAGTGGGGC 
CGAGAAGCCA TTGAAATGCA CGAGAACGGA AGCACCAAAA ACCTCCTGCA GATGACAGAC 
GTGTACTACT CGCCCACAAA CGTAAGGAAC CCTGAACTTG AACGCAACGG ACTGTACCCA 
GCCTACACTG GACTGCCCGG ATCGAGACAC TCTTGCATCT TCCCGGGACA GTATAACCCA 
TCTTTCATCA GCGACGAGAG CAGGAGGAGA GACTACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heg1 ( XM_006522717.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522780.1 Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X3, mRNA.

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CloneID	OMu52519
Clone ID Related Accession (Same CDS sequence)	XM_006522717.1 , XM_006522717.2
Accession Version	XM_006522717.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein HEG homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCGCTG CAAGGAACCG TTGGCTGGAA AGTAACAACC CCGAACCCCA TATAGGATGC 
AGTCCCTCTT ATCAGAGTCA AGAGGACCAT TCAGGTTCCA GAAAGGGAGT GACAGCTCAG 
AATGCTAGAA TGAGCCACAG TTCCTCAGAA GGCCCAGAAA ACCCCCCTCT CCTCCCTGAG 
ACAAGTGCTG AGTGGAGCAA CATGGCTTCC TCCCACAGAG CGGACATCGC TGGTCTGCGC 
CGTGGACCCT CCCCTGAGAT CACCACGGCG CCAACTGCCC ACAGCAGCTT GTTGTCCTTG 
GAGAGCCTTC CTGAGTCACC CAGCAGTTCC AGGTCACAAA GAAGAATCAC ACCTTCTCAA 
ACAGAGAGTG GGACATCCCT AGGCTTCCTG GAGAGGACGA GGGAGCTCCC AGAAGAAGGG 
ACTGTGCACA CGCAAGTGGC TGGCACCTGG GTGTCCCGCC AAGCCTCCCA CCCTGCTCTG 
GAACCTGGGG AGCCAACTGT GCTCTCCCAG AAGAGAAATT CATCCGGCCA GGAACACTCC 
GGGCCACCAT TCTCCTGGAG TCAGAGTCAC CCTCCTCCCT CAGACCACCC ATCGTCTTCT 
GGAAGTATTA AGAATGGTAA TAATTTCACC GCTCTCCAAA ACCCATCAGT CACCCAGACA 
AAGAGCATGC TTATCACTGA CACATACACT AATGGCGTCC CAAGAACTCT GCGGTCTCTG 
CCCGTTGGTG TGGACCCTGC AGATGAGACA GAGGGTTTCC CTGAGCACTC CAGACTTGGT 
ATAACTTCAA TGTCAGTCCG CTCTTCACCC TCTGTGAAGG ACTCAAGGAC AAACAGTGGA 
CTAACTGAGC ATCTGGGAGA TGGAGAAGGC ACTGAGCTGT CTACAGAAAA TGGATATGGA 
CTTCCATCTA TACACTGGCA AAGTGATGCC CCCTCCTTCG GAGGACGTCA GCTTGCCAGC 
AGCTCAGAAG CGGGCGATGG AAGGGCCATG CCTCTGACTG AGGCTGTGTT TAGATCAGAC 
CCCTCCATTG GTGGTGGAGA GAGCACCGGA CGATGGATCC TCACCAAGAA GAAAACATCT 
ACAGATGCGG CAGAAAGCTC AGCTTTGCAT CCAGAAGCTG GGGGTGCTGG GGGCTTGACT 
CAGTCCTCAC ACGCTGCACA ACAGCCCCGA GGTGGCGGCG AAGACTCAGG GATGGGTGGG 
AGGAGTTACG CAGAGTCTTC ATCTTCTTCT TCATCTACAT CTTCTTCCGA AAGTCTGGAT 
TCCTCAGCAC CTCTCAGAGA GCATTCTCCT CCGGAAGATG GTGCAATGCT GAGTGACAGC 
TCTGATCTGG CAGACAGCAC TTCGGGGGCT CGCACACCTC ACACGTCTGC AATGTCCACC 
CGGAGCGGGG AGCGCACGCT GCGGTCCCTT GACCTCTCGA GTGCAGCAAC CCGCCCTGCG 
CGCCCCACTC CACGAGGGAA CGTGACAGAG CACGCTGGCC TGCTGTCCGG GGCACCAACT 
CTCGGAGTCA CCGGACTTAG CTACACTCGA GAACATGGCT CAGACGCTGG GCAGAGGACT 
TCCAGTGACC ACACAGACCA CGGCTATGTT CCGTCTACTT TCACCAAAGG AGAACGGACG 
CTACTGTCTA TTACAGATAA CACCTCCTAC TCAGAGGCCA GCGAGAGCTC AACCTCTTCC 
GTTAAGATCT CAGACTCTCC TTCTCAGGCT CAGCCTAAAC AGAGTAGCAT GTCCTCCGAC 
GATGACGAGC CTGCTCAGTC TTCCACAGAG TCACCGGTTC TGCATACGTC CAACCTGCCA 
ACCTACACGT CCACTGTTAA TATGCCAAAC ACCTTGGTTC TTGATACCGG TACCAAACCT 
GTTGAGGACC CATCTGATTC ACGGGTGCCT TCCACTCAAC CCTCACCGTC ACAACCCCAG 
CCATTCTCAT CAGCCTTACC ATCAACCAGG TCTCCTGGGT CTACCTCTGA GACAACCACA 
TCCTCACCAT CACCTTCACC CATCTCCTTA CTGGTGTCAA CTCTTGCCCC ATATTCTGTC 
TCACAAACAA CCTTCCCACA TCCATCTTCT ACCCTAGTCC CTCACAGGCC AAGGGAGCCA 
CGTGTGACTT CAGTCCAAAT GTCGACGGCA ATATCAGCCA TTGCATTGAT CCCCAGCAAT 
CAAACAGCAA ACCCCAAGAA CCAGAGTACC CCACAACAAG AGAAACCCAT CACAGAAGCT 
AAGTCACCAA GCTTGGTGTC TCCGCCCACA GACTCTACCA AAGCAGTAAC AGTGAGCCTT 
CCTCCAGGGG CCCCTTGGTC CCCAGCCTTA ACAGGGTTCT CCACGGGGCC AGCCCTTCCA 
GCCACAAGCA CCAGTTTAGC CCAGATGTCT CCAGCTTTGA CATCTGCCAT GCCCCAGACG 
ACTCACTCTC CTGTGACCAG TCCCAGTACT CTGTCACATG TAGAAGCTTT GACTTCAGGT 
GCTGTAGTTG TGCACACTAC CCCGAAGAAA CCACATCTCC CAACCAATCC TGAAATCCTA 
GTGCCACACA TCTCAACTGA GGGGGCCATC ACCACAGAAG GGAACCGGGA GCATACGGAT 
CCTACCACTC AGCCAATCCC TCTGACCACT TCAACCACGT CAGCAGGAGA AAGGACCACA 
GAGCTGGGCC GTGCGGAAGA GTCCAGTCCA TCCCATTTTC TTACACCATC TTCACCTCAA 
ACCACAGATG TTTCTACAGC TGAAATGTTG ACTTCCAGAT ATATCACCTT TGCTGCTCAG 
AGCACCTCAC AGTCACCAAC AGCCTTGCCA CCTCTAACCC CAGTCAACAG CTGTACCGTG 
AACCCTTGTC TTCATGATGG CAAATGCATT GTGGACCTCA CTGGCCGTGG GTATCGATGT 
GTGTGCCCGC CTGCCTGGCA GGGGGAGAAC TGCAGTGTGG ATGTGAACGA ATGTCTCTCC 
AGCCCCTGTC CTCCCCTAGC CACGTGCAAC AATACTCAGG GATCGTTCAC CTGCAGATGC 
CCAGTCGGGT ATCAGTTGGA GAAAGGAATA TGCAATCTGG TTAGAACCTT TGTGACCGAG 
TTTAAGTTAA AGAAGACTTT TCTCAATACT ACTGCAGAAA ACCATTCCAA CACTCAAGAG 
CTTGAAAACG AGATCGCTCA AACACTAAAC GTGTGTTTTT CAACATTGCC CGGTTATATC 
CGAACTACAG CCCATGTGTC TAGGGAGCCC AGTACAGTTT TCATCTCACT GAAAACCACC 
TTTGCCCTGG CCTCCAATGT GACACTGTTT GACCTGGCAG ACAGGATCCA GAAATATGTC 
AACTCCTGCA GGTCCTCTGC TGAAGTCTGC CAGCTCTTGG GGTCTCAGAG GCGGGTCTTT 
CGAGCGGGCA GCTTGTGTAA GCGGAAGAGT CCAGAATGTG ACAAAGAGAC CTCCATCTGC 
ACCGACCTGG ATGGTGTCGC GCTCTGTCAG TGCAAGTCTG GCTACTTCCA GTTCAACAAG 
ATGGATCACT CTTGCCGAGC ATGTGAAGAT GGATATAGGC TTGAAAATGA AACCTGTATG 
AGTTGCCCAT TTGGCCTCGG TGGACTCAAC TGTGGAAACC CCTATCAGCT CATCACTGTG 
GTGATCGCAG CCGCAGGAGG TGGCCTCTTG CTCATCCTGG GCGTGGCACT CATTGTTACC 
TGTTGCAGCC CACAAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522780.1
CDS	183..4103
Translation	MATPRAPRWPPPSLLLLLLLPLLLLPPAAPGARGSLPSPAHRTLLPVAGPLSPPGAGHTAPGPGVATRRGRSGRVPRGVSAAAARNRWLESNNPEPHIGCSPSYQSQEDHSGSRKGVTAQNARMSHSSSEGPENPPLLPETSAEWSNMASSHRADIAGLRRGPSPEITTAPTAHSSLLSLESLPESPSSSRSQRRITPSQTESGTSLGFLERTRELPEEGTVHTQVAGTWVSRQASHPALEPGEPTVLSQKRNSSGQEHSGPPFSWSQSHPPPSDHPSSSGSIKNGNNFTALQNPSVTQTKSMLITDTYTNGVPRTLRSLPVGVDPADETEGFPEHSRLGITSMSVRSSPSVKDSRTNSGLTEHLGDGEGTELSTENGYGLPSIHWQSDAPSFGGRQLASSSEAGDGRAMPLTEAVFRSDPSIGGGESTGRWILTKKKTSTDAAESSALHPEAGGAGGLTQSSHAAQQPRGGGEDSGMGGRSYAESSSSSSSTSSSESLDSSAPLREHSPPEDGAMLSDSSDLADSTSGARTPHTSAMSTRSGERTLRSLDLSSAATRPARPTPRGNVTEHAGLLSGAPTLGVTGLSYTREHGSDAGQRTSSDHTDHGYVPSTFTKGERTLLSITDNTSYSEASESSTSSVKISDSPSQAQPKQSSMSSDDDEPAQSSTESPVLHTSNLPTYTSTVNMPNTLVLDTGTKPVEDPSDSRVPSTQPSPSQPQPFSSALPSTRSPGSTSETTTSSPSPSPISLLVSTLAPYSVSQTTFPHPSSTLVPHRPREPRVTSVQMSTAISAIALIPSNQTANPKNQSTPQQEKPITEAKSPSLVSPPTDSTKAVTVSLPPGAPWSPALTGFSTGPALPATSTSLAQMSPALTSAMPQTTHSPVTSPSTLSHVEALTSGAVVVHTTPKKPHLPTNPEILVPHISTEGAITTEGNREHTDPTTQPIPLTTSTTSAGERTTELGRAEESSPSHFLTPSSPQTTDVSTAEMLTSRYITFAAQSTSQSPTALPPLTPVNSCTVNPCLHDGKCIVDLTGRGYRCVCPPAWQGENCSVDVNECLSSPCPPLATCNNTQGSFTCRCPVGYQLEKGICNLVRTFVTEFKLKKTFLNTTAENHSNTQELENEIAQTLNVCFSTLPGYIRTTAHVSREPSTVFISLKTTFALASNVTLFDLADRIQKYVNSCRSSAEVCQLLGSQRRVFRAGSLCKRKSPECDKETSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGVALIVTCCSPQT

Target ORF information:

RefSeq Version	XM_006522717.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522717.2

ORF Insert Sequence:

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCGCTG CAAGGAACCG TTGGCTGGAA AGTAACAACC CCGAACCCCA TATAGGATGC 
AGTCCCTCTT ATCAGAGTCA AGAGGACCAT TCAGGTTCCA GAAAGGGAGT GACAGCTCAG 
AATGCTAGAA TGAGCCACAG TTCCTCAGAA GGCCCAGAAA ACCCCCCTCT CCTCCCTGAG 
ACAAGTGCTG AGTGGAGCAA CATGGCTTCC TCCCACAGAG CGGACATCGC TGGTCTGCGC 
CGTGGACCCT CCCCTGAGAT CACCACGGCG CCAACTGCCC ACAGCAGCTT GTTGTCCTTG 
GAGAGCCTTC CTGAGTCACC CAGCAGTTCC AGGTCACAAA GAAGAATCAC ACCTTCTCAA 
ACAGAGAGTG GGACATCCCT AGGCTTCCTG GAGAGGACGA GGGAGCTCCC AGAAGAAGGG 
ACTGTGCACA CGCAAGTGGC TGGCACCTGG GTGTCCCGCC AAGCCTCCCA CCCTGCTCTG 
GAACCTGGGG AGCCAACTGT GCTCTCCCAG AAGAGAAATT CATCCGGCCA GGAACACTCC 
GGGCCACCAT TCTCCTGGAG TCAGAGTCAC CCTCCTCCCT CAGACCACCC ATCGTCTTCT 
GGAAGTATTA AGAATGGTAA TAATTTCACC GCTCTCCAAA ACCCATCAGT CACCCAGACA 
AAGAGCATGC TTATCACTGA CACATACACT AATGGCGTCC CAAGAACTCT GCGGTCTCTG 
CCCGTTGGTG TGGACCCTGC AGATGAGACA GAGGGTTTCC CTGAGCACTC CAGACTTGGT 
ATAACTTCAA TGTCAGTCCG CTCTTCACCC TCTGTGAAGG ACTCAAGGAC AAACAGTGGA 
CTAACTGAGC ATCTGGGAGA TGGAGAAGGC ACTGAGCTGT CTACAGAAAA TGGATATGGA 
CTTCCATCTA TACACTGGCA AAGTGATGCC CCCTCCTTCG GAGGACGTCA GCTTGCCAGC 
AGCTCAGAAG CGGGCGATGG AAGGGCCATG CCTCTGACTG AGGCTGTGTT TAGATCAGAC 
CCCTCCATTG GTGGTGGAGA GAGCACCGGA CGATGGATCC TCACCAAGAA GAAAACATCT 
ACAGATGCGG CAGAAAGCTC AGCTTTGCAT CCAGAAGCTG GGGGTGCTGG GGGCTTGACT 
CAGTCCTCAC ACGCTGCACA ACAGCCCCGA GGTGGCGGCG AAGACTCAGG GATGGGTGGG 
AGGAGTTACG CAGAGTCTTC ATCTTCTTCT TCATCTACAT CTTCTTCCGA AAGTCTGGAT 
TCCTCAGCAC CTCTCAGAGA GCATTCTCCT CCGGAAGATG GTGCAATGCT GAGTGACAGC 
TCTGATCTGG CAGACAGCAC TTCGGGGGCT CGCACACCTC ACACGTCTGC AATGTCCACC 
CGGAGCGGGG AGCGCACGCT GCGGTCCCTT GACCTCTCGA GTGCAGCAAC CCGCCCTGCG 
CGCCCCACTC CACGAGGGAA CGTGACAGAG CACGCTGGCC TGCTGTCCGG GGCACCAACT 
CTCGGAGTCA CCGGACTTAG CTACACTCGA GAACATGGCT CAGACGCTGG GCAGAGGACT 
TCCAGTGACC ACACAGACCA CGGCTATGTT CCGTCTACTT TCACCAAAGG AGAACGGACG 
CTACTGTCTA TTACAGATAA CACCTCCTAC TCAGAGGCCA GCGAGAGCTC AACCTCTTCC 
GTTAAGATCT CAGACTCTCC TTCTCAGGCT CAGCCTAAAC AGAGTAGCAT GTCCTCCGAC 
GATGACGAGC CTGCTCAGTC TTCCACAGAG TCACCGGTTC TGCATACGTC CAACCTGCCA 
ACCTACACGT CCACTGTTAA TATGCCAAAC ACCTTGGTTC TTGATACCGG TACCAAACCT 
GTTGAGGACC CATCTGATTC ACGGGTGCCT TCCACTCAAC CCTCACCGTC ACAACCCCAG 
CCATTCTCAT CAGCCTTACC ATCAACCAGG TCTCCTGGGT CTACCTCTGA GACAACCACA 
TCCTCACCAT CACCTTCACC CATCTCCTTA CTGGTGTCAA CTCTTGCCCC ATATTCTGTC 
TCACAAACAA CCTTCCCACA TCCATCTTCT ACCCTAGTCC CTCACAGGCC AAGGGAGCCA 
CGTGTGACTT CAGTCCAAAT GTCGACGGCA ATATCAGCCA TTGCATTGAT CCCCAGCAAT 
CAAACAGCAA ACCCCAAGAA CCAGAGTACC CCACAACAAG AGAAACCCAT CACAGAAGCT 
AAGTCACCAA GCTTGGTGTC TCCGCCCACA GACTCTACCA AAGCAGTAAC AGTGAGCCTT 
CCTCCAGGGG CCCCTTGGTC CCCAGCCTTA ACAGGGTTCT CCACGGGGCC AGCCCTTCCA 
GCCACAAGCA CCAGTTTAGC CCAGATGTCT CCAGCTTTGA CATCTGCCAT GCCCCAGACG 
ACTCACTCTC CTGTGACCAG TCCCAGTACT CTGTCACATG TAGAAGCTTT GACTTCAGGT 
GCTGTAGTTG TGCACACTAC CCCGAAGAAA CCACATCTCC CAACCAATCC TGAAATCCTA 
GTGCCACACA TCTCAACTGA GGGGGCCATC ACCACAGAAG GGAACCGGGA GCATACGGAT 
CCTACCACTC AGCCAATCCC TCTGACCACT TCAACCACGT CAGCAGGAGA AAGGACCACA 
GAGCTGGGCC GTGCGGAAGA GTCCAGTCCA TCCCATTTTC TTACACCATC TTCACCTCAA 
ACCACAGATG TTTCTACAGC TGAAATGTTG ACTTCCAGAT ATATCACCTT TGCTGCTCAG 
AGCACCTCAC AGTCACCAAC AGCCTTGCCA CCTCTAACCC CAGTCAACAG CTGTACCGTG 
AACCCTTGTC TTCATGATGG CAAATGCATT GTGGACCTCA CTGGCCGTGG GTATCGATGT 
GTGTGCCCGC CTGCCTGGCA GGGGGAGAAC TGCAGTGTGG ATGTGAACGA ATGTCTCTCC 
AGCCCCTGTC CTCCCCTAGC CACGTGCAAC AATACTCAGG GATCGTTCAC CTGCAGATGC 
CCAGTCGGGT ATCAGTTGGA GAAAGGAATA TGCAATCTGG TTAGAACCTT TGTGACCGAG 
TTTAAGTTAA AGAAGACTTT TCTCAATACT ACTGCAGAAA ACCATTCCAA CACTCAAGAG 
CTTGAAAACG AGATCGCTCA AACACTAAAC GTGTGTTTTT CAACATTGCC CGGTTATATC 
CGAACTACAG CCCATGTGTC TAGGGAGCCC AGTACAGTTT TCATCTCACT GAAAACCACC 
TTTGCCCTGG CCTCCAATGT GACACTGTTT GACCTGGCAG ACAGGATCCA GAAATATGTC 
AACTCCTGCA GGTCCTCTGC TGAAGTCTGC CAGCTCTTGG GGTCTCAGAG GCGGGTCTTT 
CGAGCGGGCA GCTTGTGTAA GCGGAAGAGT CCAGAATGTG ACAAAGAGAC CTCCATCTGC 
ACCGACCTGG ATGGTGTCGC GCTCTGTCAG TGCAAGTCTG GCTACTTCCA GTTCAACAAG 
ATGGATCACT CTTGCCGAGC ATGTGAAGAT GGATATAGGC TTGAAAATGA AACCTGTATG 
AGTTGCCCAT TTGGCCTCGG TGGACTCAAC TGTGGAAACC CCTATCAGCT CATCACTGTG 
GTGATCGCAG CCGCAGGAGG TGGCCTCTTG CTCATCCTGG GCGTGGCACT CATTGTTACC 
TGTTGCAGCC CACAAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heg1 ( NM_175256.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780465.4 Mus musculus heart development protein with EGF-like domains 1 (Heg1), mRNA.

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CloneID	OMu05083
Clone ID Related Accession (Same CDS sequence)	NM_175256.5
Accession Version	NM_175256.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Mus musculus(house mouse)
Product	protein HEG homolog 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB639887.1, BE862445.1 and BC076596.1. On Jul 25, 2007 this sequence version replaced NM_175256.4. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCTTGG AGAGCCTTCC TGAGTCACCC AGCAGTTCCA GGTCACAAAG AAGAATCACA 
CCTTCTCAAA CAGAGAGTGG GACATCCCTA GGCTTCCTGG AGAGGACGAG GGAGCTCCCA 
GAAGAAGGGA CTGTGCACAC GCAAGTGGCT GGCACCTGGG TGTCCCGCCA AGCCTCCCAC 
CCTGCTCTGG AACCTGGGGA GCCAACTGTG CTCTCCCAGA AGAGAAATTC ATCCGGCCAG 
GAACACTCCG GGCCACCATT CTCCTGGAGT CAGAGTCACC CTCCTCCCTC AGACCACCCA 
TCGTCTCCGG AAGATGGTGC AATGCTGAGT GACAGCTCTG ATCTGGCAGA CAGCACTTCG 
GGGGCTCGCA CACCTCACAC GTCTGCAATG TCCACCCGGA GCGGGGAGCG CACGCTGCGG 
TCCCTTGACC TCTCGAGTGC AGCAACCCGC CCTGCGCGCC CCACTCCACG AGGGAACGTG 
ACAGAGCACG CTGGCCTGCT GTCCGGGGCA CCAACTCTCG GAGTCACCGG ACTTAGCTAC 
ACTCGAGAAC ATGGCTCAGA CGCTGGGCAG AGGACTTCCA GTGACCACAC AGACCACGGC 
TATGTTCCGT CTACTTTCAC CAAAGGAGAA CGGACGCTAC TGTCTATTAC AGATAACACC 
TCCTACTCAG AGGCCAGCGA GAGCTCAACC TCTTCCGTTA AGATCTCAGA CTCTCCTTCT 
CAGGCTCAGC CTAAACAGAG TAGCATGTCC TCCGACGATG ACGAGCCTGC TCAGTCTTCC 
ACAGAGTCAC CGGTTCTGCA TACGTCCAAC CTGCCAACCT ACACGTCCAC TGTTAATATG 
CCAAACACCT TGGTTCTTGA TACCGGTACC AAACCTGTTG AGGACCCATC TGATTCACGG 
GTGCCTTCCA CTCAACCCTC ACCGTCACAA CCCCAGCCAT TCTCATCAGC CTTACCATCA 
ACCAGGTCTC CTGGGTCTAC CTCTGAGACA ACCACATCCT CACCATCACC TTCACCCATC 
TCCTTACTGG TGTCAACTCT TGCCCCATAT TCTGTCTCAC AAACAACCTT CCCACATCCA 
TCTTCTACCC TAGTCCCTCA CAGGCCAAGG GAGCCACGTG TGACTTCAGT CCAAATGTCG 
ACGGCAATAT CAGCCATTGC ATTGATCCCC AGCAATCAAA CAGCAAACCC CAAGAACCAG 
AGTACCCCAC AACAAGAGAA ACCCATCACA GAAGCTAAGT CACCAAGCTT GGTGTCTCCG 
CCCACAGACT CTACCAAAGC AGTAACAGTG AGCCTTCCTC CAGGGGCCCC TTGGTCCCCA 
GCCTTAACAG GGTTCTCCAC GGGGCCAGCC CTTCCAGCCA CAAGCACCAG TTTAGCCCAG 
ATGTCTCCAG CTTTGACATC TGCCATGCCC CAGACGACTC ACTCTCCTGT GACCAGTCCC 
AGTACTCTGT CACATGTAGA AGCTTTGACT TCAGGTGCTG TAGTTGTGCA CACTACCCCG 
AAGAAACCAC ATCTCCCAAC CAATCCTGAA ATCCTAGTGC CACACATCTC AACTGAGGGG 
GCCATCACCA CAGAAGGGAA CCGGGAGCAT ACGGATCCTA CCACTCAGCC AATCCCTCTG 
ACCACTTCAA CCACGTCAGC AGGAGAAAGG ACCACAGAGC TGGGCCGTGC GGAAGAGTCC 
AGTCCATCCC ATTTTCTTAC ACCATCTTCA CCTCAAACCA CAGATGTTTC TACAGCTGAA 
ATGTTGACTT CCAGATATAT CACCTTTGCT GCTCAGAGCA CCTCACAGTC ACCAACAGCC 
TTGCCACCTC TAACCCCAGT CAACAGCTGT ACCGTGAACC CTTGTCTTCA TGATGGCAAA 
TGCATTGTGG ACCTCACTGG CCGTGGGTAT CGATGTGTGT GCCCGCCTGC CTGGCAGGGG 
GAGAACTGCA GTGTGGATGT GAACGAATGT CTCTCCAGCC CCTGTCCTCC CCTAGCCACG 
TGCAACAATA CTCAGGGATC GTTCACCTGC AGATGCCCAG TCGGGTATCA GTTGGAGAAA 
GGAATATGCA ATCTGGTTAG AACCTTTGTG ACCGAGTTTA AGTTAAAGAA GACTTTTCTC 
AATACTACTG CAGAAAACCA TTCCAACACT CAAGAGCTTG AAAACGAGAT CGCTCAAACA 
CTAAACGTGT GTTTTTCAAC ATTGCCCGGT TATATCCGAA CTACAGCCCA TGTGTCTAGG 
GAGCCCAGTA CAGTTTTCAT CTCACTGAAA ACCACCTTTG CCCTGGCCTC CAATGTGACA 
CTGTTTGACC TGGCAGACAG GATCCAGAAA TATGTCAACT CCTGCAGGTC CTCTGCTGAA 
GTCTGCCAGC TCTTGGGGTC TCAGAGGCGG GTCTTTCGAG CGGGCAGCTT GTGTAAGCGG 
AAGAGTCCAG AATGTGACAA AGAGACCTCC ATCTGCACCG ACCTGGATGG TGTCGCGCTC 
TGTCAGTGCA AGTCTGGCTA CTTCCAGTTC AACAAGATGG ATCACTCTTG CCGAGCATGT 
GAAGATGGAT ATAGGCTTGA AAATGAAACC TGTATGAGTT GCCCATTTGG CCTCGGTGGA 
CTCAACTGTG GAAACCCCTA TCAGCTCATC ACTGTGGTGA TCGCAGCCGC AGGAGGTGGC 
CTCTTGCTCA TCCTGGGCGT GGCACTCATT GTTACCTGTT GCAGAAAGAG TAAAAATGAC 
ATAAGTAAAC TCATCTTCAA AAGTGGGGAC TTCCAAATGT CCCCATACAC CGATGTCCCC 
AAGAATCCTC GCTCGCAAGA GTGGGGCCGA GAAGCCATTG AAATGCACGA GAACGGAAGC 
ACCAAAAACC TCCTGCAGAT GACAGACGTG TACTACTCGC CCACAAACGT AAGGAACCCT 
GAACTTGAAC GCAACGGACT GTACCCAGCC TACACTGGAC TGCCCGGATC GAGACACTCT 
TGCATCTTCC CGGGACAGTA TAACCCATCT TTCATCAGCG ACGAGAGCAG GAGGAGAGAC 
TACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780465.4
CDS	147..3395
Translation	MATPRAPRWPPPSLLLLLLLPLLLLPPAAPGARGSLPSPAHRTLLPVAGPLSPPGAGHTAPGPGVATRRGRSGRVPRGVSAALESLPESPSSSRSQRRITPSQTESGTSLGFLERTRELPEEGTVHTQVAGTWVSRQASHPALEPGEPTVLSQKRNSSGQEHSGPPFSWSQSHPPPSDHPSSPEDGAMLSDSSDLADSTSGARTPHTSAMSTRSGERTLRSLDLSSAATRPARPTPRGNVTEHAGLLSGAPTLGVTGLSYTREHGSDAGQRTSSDHTDHGYVPSTFTKGERTLLSITDNTSYSEASESSTSSVKISDSPSQAQPKQSSMSSDDDEPAQSSTESPVLHTSNLPTYTSTVNMPNTLVLDTGTKPVEDPSDSRVPSTQPSPSQPQPFSSALPSTRSPGSTSETTTSSPSPSPISLLVSTLAPYSVSQTTFPHPSSTLVPHRPREPRVTSVQMSTAISAIALIPSNQTANPKNQSTPQQEKPITEAKSPSLVSPPTDSTKAVTVSLPPGAPWSPALTGFSTGPALPATSTSLAQMSPALTSAMPQTTHSPVTSPSTLSHVEALTSGAVVVHTTPKKPHLPTNPEILVPHISTEGAITTEGNREHTDPTTQPIPLTTSTTSAGERTTELGRAEESSPSHFLTPSSPQTTDVSTAEMLTSRYITFAAQSTSQSPTALPPLTPVNSCTVNPCLHDGKCIVDLTGRGYRCVCPPAWQGENCSVDVNECLSSPCPPLATCNNTQGSFTCRCPVGYQLEKGICNLVRTFVTEFKLKKTFLNTTAENHSNTQELENEIAQTLNVCFSTLPGYIRTTAHVSREPSTVFISLKTTFALASNVTLFDLADRIQKYVNSCRSSAEVCQLLGSQRRVFRAGSLCKRKSPECDKETSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGVALIVTCCRKSKNDISKLIFKSGDFQMSPYTDVPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTNVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

Target ORF information:

RefSeq Version	NM_175256.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus heart development protein with EGF-like domains 1 (Heg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175256.5

ORF Insert Sequence:

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCTTGG AGAGCCTTCC TGAGTCACCC AGCAGTTCCA GGTCACAAAG AAGAATCACA 
CCTTCTCAAA CAGAGAGTGG GACATCCCTA GGCTTCCTGG AGAGGACGAG GGAGCTCCCA 
GAAGAAGGGA CTGTGCACAC GCAAGTGGCT GGCACCTGGG TGTCCCGCCA AGCCTCCCAC 
CCTGCTCTGG AACCTGGGGA GCCAACTGTG CTCTCCCAGA AGAGAAATTC ATCCGGCCAG 
GAACACTCCG GGCCACCATT CTCCTGGAGT CAGAGTCACC CTCCTCCCTC AGACCACCCA 
TCGTCTCCGG AAGATGGTGC AATGCTGAGT GACAGCTCTG ATCTGGCAGA CAGCACTTCG 
GGGGCTCGCA CACCTCACAC GTCTGCAATG TCCACCCGGA GCGGGGAGCG CACGCTGCGG 
TCCCTTGACC TCTCGAGTGC AGCAACCCGC CCTGCGCGCC CCACTCCACG AGGGAACGTG 
ACAGAGCACG CTGGCCTGCT GTCCGGGGCA CCAACTCTCG GAGTCACCGG ACTTAGCTAC 
ACTCGAGAAC ATGGCTCAGA CGCTGGGCAG AGGACTTCCA GTGACCACAC AGACCACGGC 
TATGTTCCGT CTACTTTCAC CAAAGGAGAA CGGACGCTAC TGTCTATTAC AGATAACACC 
TCCTACTCAG AGGCCAGCGA GAGCTCAACC TCTTCCGTTA AGATCTCAGA CTCTCCTTCT 
CAGGCTCAGC CTAAACAGAG TAGCATGTCC TCCGACGATG ACGAGCCTGC TCAGTCTTCC 
ACAGAGTCAC CGGTTCTGCA TACGTCCAAC CTGCCAACCT ACACGTCCAC TGTTAATATG 
CCAAACACCT TGGTTCTTGA TACCGGTACC AAACCTGTTG AGGACCCATC TGATTCACGG 
GTGCCTTCCA CTCAACCCTC ACCGTCACAA CCCCAGCCAT TCTCATCAGC CTTACCATCA 
ACCAGGTCTC CTGGGTCTAC CTCTGAGACA ACCACATCCT CACCATCACC TTCACCCATC 
TCCTTACTGG TGTCAACTCT TGCCCCATAT TCTGTCTCAC AAACAACCTT CCCACATCCA 
TCTTCTACCC TAGTCCCTCA CAGGCCAAGG GAGCCACGTG TGACTTCAGT CCAAATGTCG 
ACGGCAATAT CAGCCATTGC ATTGATCCCC AGCAATCAAA CAGCAAACCC CAAGAACCAG 
AGTACCCCAC AACAAGAGAA ACCCATCACA GAAGCTAAGT CACCAAGCTT GGTGTCTCCG 
CCCACAGACT CTACCAAAGC AGTAACAGTG AGCCTTCCTC CAGGGGCCCC TTGGTCCCCA 
GCCTTAACAG GGTTCTCCAC GGGGCCAGCC CTTCCAGCCA CAAGCACCAG TTTAGCCCAG 
ATGTCTCCAG CTTTGACATC TGCCATGCCC CAGACGACTC ACTCTCCTGT GACCAGTCCC 
AGTACTCTGT CACATGTAGA AGCTTTGACT TCAGGTGCTG TAGTTGTGCA CACTACCCCG 
AAGAAACCAC ATCTCCCAAC CAATCCTGAA ATCCTAGTGC CACACATCTC AACTGAGGGG 
GCCATCACCA CAGAAGGGAA CCGGGAGCAT ACGGATCCTA CCACTCAGCC AATCCCTCTG 
ACCACTTCAA CCACGTCAGC AGGAGAAAGG ACCACAGAGC TGGGCCGTGC GGAAGAGTCC 
AGTCCATCCC ATTTTCTTAC ACCATCTTCA CCTCAAACCA CAGATGTTTC TACAGCTGAA 
ATGTTGACTT CCAGATATAT CACCTTTGCT GCTCAGAGCA CCTCACAGTC ACCAACAGCC 
TTGCCACCTC TAACCCCAGT CAACAGCTGT ACCGTGAACC CTTGTCTTCA TGATGGCAAA 
TGCATTGTGG ACCTCACTGG CCGTGGGTAT CGATGTGTGT GCCCGCCTGC CTGGCAGGGG 
GAGAACTGCA GTGTGGATGT GAACGAATGT CTCTCCAGCC CCTGTCCTCC CCTAGCCACG 
TGCAACAATA CTCAGGGATC GTTCACCTGC AGATGCCCAG TCGGGTATCA GTTGGAGAAA 
GGAATATGCA ATCTGGTTAG AACCTTTGTG ACCGAGTTTA AGTTAAAGAA GACTTTTCTC 
AATACTACTG CAGAAAACCA TTCCAACACT CAAGAGCTTG AAAACGAGAT CGCTCAAACA 
CTAAACGTGT GTTTTTCAAC ATTGCCCGGT TATATCCGAA CTACAGCCCA TGTGTCTAGG 
GAGCCCAGTA CAGTTTTCAT CTCACTGAAA ACCACCTTTG CCCTGGCCTC CAATGTGACA 
CTGTTTGACC TGGCAGACAG GATCCAGAAA TATGTCAACT CCTGCAGGTC CTCTGCTGAA 
GTCTGCCAGC TCTTGGGGTC TCAGAGGCGG GTCTTTCGAG CGGGCAGCTT GTGTAAGCGG 
AAGAGTCCAG AATGTGACAA AGAGACCTCC ATCTGCACCG ACCTGGATGG TGTCGCGCTC 
TGTCAGTGCA AGTCTGGCTA CTTCCAGTTC AACAAGATGG ATCACTCTTG CCGAGCATGT 
GAAGATGGAT ATAGGCTTGA AAATGAAACC TGTATGAGTT GCCCATTTGG CCTCGGTGGA 
CTCAACTGTG GAAACCCCTA TCAGCTCATC ACTGTGGTGA TCGCAGCCGC AGGAGGTGGC 
CTCTTGCTCA TCCTGGGCGT GGCACTCATT GTTACCTGTT GCAGAAAGAG TAAAAATGAC 
ATAAGTAAAC TCATCTTCAA AAGTGGGGAC TTCCAAATGT CCCCATACAC CGATGTCCCC 
AAGAATCCTC GCTCGCAAGA GTGGGGCCGA GAAGCCATTG AAATGCACGA GAACGGAAGC 
ACCAAAAACC TCCTGCAGAT GACAGACGTG TACTACTCGC CCACAAACGT AAGGAACCCT 
GAACTTGAAC GCAACGGACT GTACCCAGCC TACACTGGAC TGCCCGGATC GAGACACTCT 
TGCATCTTCC CGGGACAGTA TAACCCATCT TTCATCAGCG ACGAGAGCAG GAGGAGAGAC 
TACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heg1 ( XM_006522715.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522778.1 Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu52517
Clone ID Related Accession (Same CDS sequence)	XM_006522715.3
Accession Version	XM_006522715.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein HEG homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCGCTG CAAGGAACCG TTGGCTGGAA AGTAACAACC CCGAACCCCA TATAGGATGC 
AGTCCCTCTT ATCAGAGTCA AGAGGACCAT TCAGGTTCCA GAAAGGGAGT GACAGCTCAG 
AATGCTAGAA TGAGCCACAG TTCCTCAGAA GGCCCAGAAA ACCCCCCTCT CCTCCCTGAG 
ACAAGTGCTG AGTGGAGCAA CATGGCTTCC TCCCACAGAG CGGACATCGC TGGTCTGCGC 
CGTGGACCCT CCCCTGAGAT CACCACGGCG CCAACTGCCC ACAGCAGCTT GTTGTCCTTG 
GAGAGCCTTC CTGAGTCACC CAGCAGTTCC AGGTCACAAA GAAGAATCAC ACCTTCTCAA 
ACAGAGAGTG GGACATCCCT AGGCTTCCTG GAGAGGACGA GGGAGCTCCC AGAAGAAGGG 
ACTGTGCACA CGCAAGTGGC TGGCACCTGG GTGTCCCGCC AAGCCTCCCA CCCTGCTCTG 
GAACCTGGGG AGCCAACTGT GCTCTCCCAG AAGAGAAATT CATCCGGCCA GGAACACTCC 
GGGCCACCAT TCTCCTGGAG TCAGAGTCAC CCTCCTCCCT CAGACCACCC ATCGTCTTCT 
GGAAGTATTA AGAATGGTAA TAATTTCACC GCTCTCCAAA ACCCATCAGT CACCCAGACA 
AAGAGCATGC TTATCACTGA CACATACACT AATGGCGTCC CAAGAACTCT GCGGTCTCTG 
CCCGTTGGTG TGGACCCTGC AGATGAGACA GAGGGTTTCC CTGAGCACTC CAGACTTGGT 
ATAACTTCAA TGTCAGTCCG CTCTTCACCC TCTGTGAAGG ACTCAAGGAC AAACAGTGGA 
CTAACTGAGC ATCTGGGAGA TGGAGAAGGC ACTGAGCTGT CTACAGAAAA TGGATATGGA 
CTTCCATCTA TACACTGGCA AAGTGATGCC CCCTCCTTCG GAGGACGTCA GCTTGCCAGC 
AGCTCAGAAG CGGGCGATGG AAGGGCCATG CCTCTGACTG AGGCTGTGTT TAGATCAGAC 
CCCTCCATTG GTGGTGGAGA GAGCACCGGA CGATGGATCC TCACCAAGAA GAAAACATCT 
ACAGATGCGG CAGAAAGCTC AGCTTTGCAT CCAGAAGCTG GGGGTGCTGG GGGCTTGACT 
CAGTCCTCAC ACGCTGCACA ACAGCCCCGA GGTGGCGGCG AAGACTCAGG GATGGGTGGG 
AGGAGTTACG CAGAGTCTTC ATCTTCTTCT TCATCTACAT CTTCTTCCGA AAGTCTGGAT 
TCCTCAGCAC CTCTCAGAGA GCATTCTCCT CCGGAAGATG GTGCAATGCT GAGTGACAGC 
TCTGATCTGG CAGACAGCAC TTCGGGGGCT CGCACACCTC ACACGTCTGC AATGTCCACC 
CGGAGCGGGG AGCGCACGCT GCGGTCCCTT GACCTCTCGA GTGCAGCAAC CCGCCCTGCG 
CGCCCCACTC CACGAGGGAA CGTGACAGAG CACGCTGGCC TGCTGTCCGG GGCACCAACT 
CTCGGAGTCA CCGGACTTAG CTACACTCGA GAACATGGCT CAGACGCTGG GCAGAGGACT 
TCCAGTGACC ACACAGACCA CGGCTATGTT CCGTCTACTT TCACCAAAGG AGAACGGACG 
CTACTGTCTA TTACAGATAA CACCTCCTAC TCAGAGGCCA GCGAGAGCTC AACCTCTTCC 
GTTAAGATCT CAGACTCTCC TTCTCAGGCT CAGCCTAAAC AGAGTAGCAT GTCCTCCGAC 
GATGACGAGC CTGCTCAGTC TTCCACAGAG TCACCGGTTC TGCATACGTC CAACCTGCCA 
ACCTACACGT CCACTGTTAA TATGCCAAAC ACCTTGGTTC TTGATACCGG TACCAAACCT 
GTTGAGGACC CATCTGATTC ACGGGTGCCT TCCACTCAAC CCTCACCGTC ACAACCCCAG 
CCATTCTCAT CAGCCTTACC ATCAACCAGG TCTCCTGGGT CTACCTCTGA GACAACCACA 
TCCTCACCAT CACCTTCACC CATCTCCTTA CTGGTGTCAA CTCTTGCCCC ATATTCTGTC 
TCACAAACAA CCTTCCCACA TCCATCTTCT ACCCTAGTCC CTCACAGGCC AAGGGAGCCA 
CGTGTGACTT CAGTCCAAAT GTCGACGGCA ATATCAGCCA TTGCATTGAT CCCCAGCAAT 
CAAACAGCAA ACCCCAAGAA CCAGAGTACC CCACAACAAG AGAAACCCAT CACAGAAGCT 
AAGTCACCAA GCTTGGTGTC TCCGCCCACA GACTCTACCA AAGCAGTAAC AGTGAGCCTT 
CCTCCAGGGG CCCCTTGGTC CCCAGCCTTA ACAGGGTTCT CCACGGGGCC AGCCCTTCCA 
GCCACAAGCA CCAGTTTAGC CCAGATGTCT CCAGCTTTGA CATCTGCCAT GCCCCAGACG 
ACTCACTCTC CTGTGACCAG TCCCAGTACT CTGTCACATG TAGAAGCTTT GACTTCAGGT 
GCTGTAGTTG TGCACACTAC CCCGAAGAAA CCACATCTCC CAACCAATCC TGAAATCCTA 
GTGCCACACA TCTCAACTGA GGGGGCCATC ACCACAGAAG GGAACCGGGA GCATACGGAT 
CCTACCACTC AGCCAATCCC TCTGACCACT TCAACCACGT CAGCAGGAGA AAGGACCACA 
GAGCTGGGCC GTGCGGAAGA GTCCAGTCCA TCCCATTTTC TTACACCATC TTCACCTCAA 
ACCACAGATG TTTCTACAGC TGAAATGTTG ACTTCCAGAT ATATCACCTT TGCTGCTCAG 
AGCACCTCAC AGTCACCAAC AGCCTTGCCA CCTCTAACCC CAGTCAACAG CTGTACCGTG 
AACCCTTGTC TTCATGATGG CAAATGCATT GTGGACCTCA CTGGCCGTGG GTATCGATGT 
GTGTGCCCGC CTGCCTGGCA GGGGGAGAAC TGCAGTGTGG ATGTGAACGA ATGTCTCTCC 
AGCCCCTGTC CTCCCCTAGC CACGTGCAAC AATACTCAGG GATCGTTCAC CTGCAGATGC 
CCAGTCGGGT ATCAGTTGGA GAAAGGAATA TGCAATCTGG TTAGAACCTT TGTGACCGAG 
TTTAAGTTAA AGAAGACTTT TCTCAATACT ACTGCAGAAA ACCATTCCAA CACTCAAGAG 
CTTGAAAACG AGATCGCTCA AACACTAAAC GTGTGTTTTT CAACATTGCC CGGTTATATC 
CGAACTACAG CCCATGTGTC TAGGGAGCCC AGTACAGTTT TCATCTCACT GAAAACCACC 
TTTGCCCTGG CCTCCAATGT GACACTGTTT GACCTGGCAG ACAGGATCCA GAAATATGTC 
AACTCCTGCA GGTCCTCTGC TGAAGTCTGC CAGCTCTTGG GGTCTCAGAG GCGGGTCTTT 
CGAGCGGGCA GCTTGTGTAA GCGGAAGAGT CCAGAATGTG ACAAAGAGAC CTCCATCTGC 
ACCGACCTGG ATGGTGTCGC GCTCTGTCAG TGCAAGTCTG GCTACTTCCA GTTCAACAAG 
ATGGATCACT CTTGCCGAGC ATGTGAAGAT GGATATAGGC TTGAAAATGA AACCTGTATG 
AGTTGCCCAT TTGGCCTCGG TGGACTCAAC TGTGGAAACC CCTATCAGCT CATCACTGTG 
GTGATCGCAG CCGCAGGAGG TGGCCTCTTG CTCATCCTGG GCGTGGCACT CATTGTTACC 
TGTTGCAGAA AGAGTAAAAA TGACATAAGT AAACTCATCT TCAAAAGTGG GGACTTCCAA 
ATGTCCCCAT ACACCGATGT CCCCAAGAAT CCTCGCTCGC AAGAGTGGGG CCGAGAAGCC 
ATTGAAATGC ACGAGAACGG AAGCACCAAA AACCTCCTGC AGATGACAGA CGTGTACTAC 
TCGCCCACAA ACGTAAGGAA CCCTGAACTT GAACGCAACG GACTGTACCC AGCCTACACT 
GGACTGCCCG GATCGAGACA CTCTTGCATC TTCCCGGGAC AGTATAACCC ATCTTTCATC 
AGCGACGAGA GCAGGAGGAG AGACTACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522778.1
CDS	182..4414
Translation	MATPRAPRWPPPSLLLLLLLPLLLLPPAAPGARGSLPSPAHRTLLPVAGPLSPPGAGHTAPGPGVATRRGRSGRVPRGVSAAAARNRWLESNNPEPHIGCSPSYQSQEDHSGSRKGVTAQNARMSHSSSEGPENPPLLPETSAEWSNMASSHRADIAGLRRGPSPEITTAPTAHSSLLSLESLPESPSSSRSQRRITPSQTESGTSLGFLERTRELPEEGTVHTQVAGTWVSRQASHPALEPGEPTVLSQKRNSSGQEHSGPPFSWSQSHPPPSDHPSSSGSIKNGNNFTALQNPSVTQTKSMLITDTYTNGVPRTLRSLPVGVDPADETEGFPEHSRLGITSMSVRSSPSVKDSRTNSGLTEHLGDGEGTELSTENGYGLPSIHWQSDAPSFGGRQLASSSEAGDGRAMPLTEAVFRSDPSIGGGESTGRWILTKKKTSTDAAESSALHPEAGGAGGLTQSSHAAQQPRGGGEDSGMGGRSYAESSSSSSSTSSSESLDSSAPLREHSPPEDGAMLSDSSDLADSTSGARTPHTSAMSTRSGERTLRSLDLSSAATRPARPTPRGNVTEHAGLLSGAPTLGVTGLSYTREHGSDAGQRTSSDHTDHGYVPSTFTKGERTLLSITDNTSYSEASESSTSSVKISDSPSQAQPKQSSMSSDDDEPAQSSTESPVLHTSNLPTYTSTVNMPNTLVLDTGTKPVEDPSDSRVPSTQPSPSQPQPFSSALPSTRSPGSTSETTTSSPSPSPISLLVSTLAPYSVSQTTFPHPSSTLVPHRPREPRVTSVQMSTAISAIALIPSNQTANPKNQSTPQQEKPITEAKSPSLVSPPTDSTKAVTVSLPPGAPWSPALTGFSTGPALPATSTSLAQMSPALTSAMPQTTHSPVTSPSTLSHVEALTSGAVVVHTTPKKPHLPTNPEILVPHISTEGAITTEGNREHTDPTTQPIPLTTSTTSAGERTTELGRAEESSPSHFLTPSSPQTTDVSTAEMLTSRYITFAAQSTSQSPTALPPLTPVNSCTVNPCLHDGKCIVDLTGRGYRCVCPPAWQGENCSVDVNECLSSPCPPLATCNNTQGSFTCRCPVGYQLEKGICNLVRTFVTEFKLKKTFLNTTAENHSNTQELENEIAQTLNVCFSTLPGYIRTTAHVSREPSTVFISLKTTFALASNVTLFDLADRIQKYVNSCRSSAEVCQLLGSQRRVFRAGSLCKRKSPECDKETSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGVALIVTCCRKSKNDISKLIFKSGDFQMSPYTDVPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTNVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

Target ORF information:

RefSeq Version	XM_006522715.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522715.3

ORF Insert Sequence:

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCGCTG CAAGGAACCG TTGGCTGGAA AGTAACAACC CCGAACCCCA TATAGGATGC 
AGTCCCTCTT ATCAGAGTCA AGAGGACCAT TCAGGTTCCA GAAAGGGAGT GACAGCTCAG 
AATGCTAGAA TGAGCCACAG TTCCTCAGAA GGCCCAGAAA ACCCCCCTCT CCTCCCTGAG 
ACAAGTGCTG AGTGGAGCAA CATGGCTTCC TCCCACAGAG CGGACATCGC TGGTCTGCGC 
CGTGGACCCT CCCCTGAGAT CACCACGGCG CCAACTGCCC ACAGCAGCTT GTTGTCCTTG 
GAGAGCCTTC CTGAGTCACC CAGCAGTTCC AGGTCACAAA GAAGAATCAC ACCTTCTCAA 
ACAGAGAGTG GGACATCCCT AGGCTTCCTG GAGAGGACGA GGGAGCTCCC AGAAGAAGGG 
ACTGTGCACA CGCAAGTGGC TGGCACCTGG GTGTCCCGCC AAGCCTCCCA CCCTGCTCTG 
GAACCTGGGG AGCCAACTGT GCTCTCCCAG AAGAGAAATT CATCCGGCCA GGAACACTCC 
GGGCCACCAT TCTCCTGGAG TCAGAGTCAC CCTCCTCCCT CAGACCACCC ATCGTCTTCT 
GGAAGTATTA AGAATGGTAA TAATTTCACC GCTCTCCAAA ACCCATCAGT CACCCAGACA 
AAGAGCATGC TTATCACTGA CACATACACT AATGGCGTCC CAAGAACTCT GCGGTCTCTG 
CCCGTTGGTG TGGACCCTGC AGATGAGACA GAGGGTTTCC CTGAGCACTC CAGACTTGGT 
ATAACTTCAA TGTCAGTCCG CTCTTCACCC TCTGTGAAGG ACTCAAGGAC AAACAGTGGA 
CTAACTGAGC ATCTGGGAGA TGGAGAAGGC ACTGAGCTGT CTACAGAAAA TGGATATGGA 
CTTCCATCTA TACACTGGCA AAGTGATGCC CCCTCCTTCG GAGGACGTCA GCTTGCCAGC 
AGCTCAGAAG CGGGCGATGG AAGGGCCATG CCTCTGACTG AGGCTGTGTT TAGATCAGAC 
CCCTCCATTG GTGGTGGAGA GAGCACCGGA CGATGGATCC TCACCAAGAA GAAAACATCT 
ACAGATGCGG CAGAAAGCTC AGCTTTGCAT CCAGAAGCTG GGGGTGCTGG GGGCTTGACT 
CAGTCCTCAC ACGCTGCACA ACAGCCCCGA GGTGGCGGCG AAGACTCAGG GATGGGTGGG 
AGGAGTTACG CAGAGTCTTC ATCTTCTTCT TCATCTACAT CTTCTTCCGA AAGTCTGGAT 
TCCTCAGCAC CTCTCAGAGA GCATTCTCCT CCGGAAGATG GTGCAATGCT GAGTGACAGC 
TCTGATCTGG CAGACAGCAC TTCGGGGGCT CGCACACCTC ACACGTCTGC AATGTCCACC 
CGGAGCGGGG AGCGCACGCT GCGGTCCCTT GACCTCTCGA GTGCAGCAAC CCGCCCTGCG 
CGCCCCACTC CACGAGGGAA CGTGACAGAG CACGCTGGCC TGCTGTCCGG GGCACCAACT 
CTCGGAGTCA CCGGACTTAG CTACACTCGA GAACATGGCT CAGACGCTGG GCAGAGGACT 
TCCAGTGACC ACACAGACCA CGGCTATGTT CCGTCTACTT TCACCAAAGG AGAACGGACG 
CTACTGTCTA TTACAGATAA CACCTCCTAC TCAGAGGCCA GCGAGAGCTC AACCTCTTCC 
GTTAAGATCT CAGACTCTCC TTCTCAGGCT CAGCCTAAAC AGAGTAGCAT GTCCTCCGAC 
GATGACGAGC CTGCTCAGTC TTCCACAGAG TCACCGGTTC TGCATACGTC CAACCTGCCA 
ACCTACACGT CCACTGTTAA TATGCCAAAC ACCTTGGTTC TTGATACCGG TACCAAACCT 
GTTGAGGACC CATCTGATTC ACGGGTGCCT TCCACTCAAC CCTCACCGTC ACAACCCCAG 
CCATTCTCAT CAGCCTTACC ATCAACCAGG TCTCCTGGGT CTACCTCTGA GACAACCACA 
TCCTCACCAT CACCTTCACC CATCTCCTTA CTGGTGTCAA CTCTTGCCCC ATATTCTGTC 
TCACAAACAA CCTTCCCACA TCCATCTTCT ACCCTAGTCC CTCACAGGCC AAGGGAGCCA 
CGTGTGACTT CAGTCCAAAT GTCGACGGCA ATATCAGCCA TTGCATTGAT CCCCAGCAAT 
CAAACAGCAA ACCCCAAGAA CCAGAGTACC CCACAACAAG AGAAACCCAT CACAGAAGCT 
AAGTCACCAA GCTTGGTGTC TCCGCCCACA GACTCTACCA AAGCAGTAAC AGTGAGCCTT 
CCTCCAGGGG CCCCTTGGTC CCCAGCCTTA ACAGGGTTCT CCACGGGGCC AGCCCTTCCA 
GCCACAAGCA CCAGTTTAGC CCAGATGTCT CCAGCTTTGA CATCTGCCAT GCCCCAGACG 
ACTCACTCTC CTGTGACCAG TCCCAGTACT CTGTCACATG TAGAAGCTTT GACTTCAGGT 
GCTGTAGTTG TGCACACTAC CCCGAAGAAA CCACATCTCC CAACCAATCC TGAAATCCTA 
GTGCCACACA TCTCAACTGA GGGGGCCATC ACCACAGAAG GGAACCGGGA GCATACGGAT 
CCTACCACTC AGCCAATCCC TCTGACCACT TCAACCACGT CAGCAGGAGA AAGGACCACA 
GAGCTGGGCC GTGCGGAAGA GTCCAGTCCA TCCCATTTTC TTACACCATC TTCACCTCAA 
ACCACAGATG TTTCTACAGC TGAAATGTTG ACTTCCAGAT ATATCACCTT TGCTGCTCAG 
AGCACCTCAC AGTCACCAAC AGCCTTGCCA CCTCTAACCC CAGTCAACAG CTGTACCGTG 
AACCCTTGTC TTCATGATGG CAAATGCATT GTGGACCTCA CTGGCCGTGG GTATCGATGT 
GTGTGCCCGC CTGCCTGGCA GGGGGAGAAC TGCAGTGTGG ATGTGAACGA ATGTCTCTCC 
AGCCCCTGTC CTCCCCTAGC CACGTGCAAC AATACTCAGG GATCGTTCAC CTGCAGATGC 
CCAGTCGGGT ATCAGTTGGA GAAAGGAATA TGCAATCTGG TTAGAACCTT TGTGACCGAG 
TTTAAGTTAA AGAAGACTTT TCTCAATACT ACTGCAGAAA ACCATTCCAA CACTCAAGAG 
CTTGAAAACG AGATCGCTCA AACACTAAAC GTGTGTTTTT CAACATTGCC CGGTTATATC 
CGAACTACAG CCCATGTGTC TAGGGAGCCC AGTACAGTTT TCATCTCACT GAAAACCACC 
TTTGCCCTGG CCTCCAATGT GACACTGTTT GACCTGGCAG ACAGGATCCA GAAATATGTC 
AACTCCTGCA GGTCCTCTGC TGAAGTCTGC CAGCTCTTGG GGTCTCAGAG GCGGGTCTTT 
CGAGCGGGCA GCTTGTGTAA GCGGAAGAGT CCAGAATGTG ACAAAGAGAC CTCCATCTGC 
ACCGACCTGG ATGGTGTCGC GCTCTGTCAG TGCAAGTCTG GCTACTTCCA GTTCAACAAG 
ATGGATCACT CTTGCCGAGC ATGTGAAGAT GGATATAGGC TTGAAAATGA AACCTGTATG 
AGTTGCCCAT TTGGCCTCGG TGGACTCAAC TGTGGAAACC CCTATCAGCT CATCACTGTG 
GTGATCGCAG CCGCAGGAGG TGGCCTCTTG CTCATCCTGG GCGTGGCACT CATTGTTACC 
TGTTGCAGAA AGAGTAAAAA TGACATAAGT AAACTCATCT TCAAAAGTGG GGACTTCCAA 
ATGTCCCCAT ACACCGATGT CCCCAAGAAT CCTCGCTCGC AAGAGTGGGG CCGAGAAGCC 
ATTGAAATGC ACGAGAACGG AAGCACCAAA AACCTCCTGC AGATGACAGA CGTGTACTAC 
TCGCCCACAA ACGTAAGGAA CCCTGAACTT GAACGCAACG GACTGTACCC AGCCTACACT 
GGACTGCCCG GATCGAGACA CTCTTGCATC TTCCCGGGAC AGTATAACCC ATCTTTCATC 
AGCGACGAGA GCAGGAGGAG AGACTACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heg1 ( XM_006522716.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522779.1 Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X2, mRNA.

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CloneID	OMu52518
Clone ID Related Accession (Same CDS sequence)	XM_006522716.1 , XM_006522716.2
Accession Version	XM_006522716.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein HEG homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCTTGG AGAGCCTTCC TGAGTCACCC AGCAGTTCCA GGTCACAAAG AAGAATCACA 
CCTTCTCAAA CAGAGAGTGG GACATCCCTA GGCTTCCTGG AGAGGACGAG GGAGCTCCCA 
GAAGAAGGGA CTGTGCACAC GCAAGTGGCT GGCACCTGGG TGTCCCGCCA AGCCTCCCAC 
CCTGCTCTGG AACCTGGGGA GCCAACTGTG CTCTCCCAGA AGAGAAATTC ATCCGGCCAG 
GAACACTCCG GGCCACCATT CTCCTGGAGT CAGAGTCACC CTCCTCCCTC AGACCACCCA 
TCGTCTTCTG GAAGTATTAA GAATGGTAAT AATTTCACCG CTCTCCAAAA CCCATCAGTC 
ACCCAGACAA AGAGCATGCT TATCACTGAC ACATACACTA ATGGCGTCCC AAGAACTCTG 
CGGTCTCTGC CCGTTGGTGT GGACCCTGCA GATGAGACAG AGGGTTTCCC TGAGCACTCC 
AGACTTGGTA TAACTTCAAT GTCAGTCCGC TCTTCACCCT CTGTGAAGGA CTCAAGGACA 
AACAGTGGAC TAACTGAGCA TCTGGGAGAT GGAGAAGGCA CTGAGCTGTC TACAGAAAAT 
GGATATGGAC TTCCATCTAT ACACTGGCAA AGTGATGCCC CCTCCTTCGG AGGACGTCAG 
CTTGCCAGCA GCTCAGAAGC GGGCGATGGA AGGGCCATGC CTCTGACTGA GGCTGTGTTT 
AGATCAGACC CCTCCATTGG TGGTGGAGAG AGCACCGGAC GATGGATCCT CACCAAGAAG 
AAAACATCTA CAGATGCGGC AGAAAGCTCA GCTTTGCATC CAGAAGCTGG GGGTGCTGGG 
GGCTTGACTC AGTCCTCACA CGCTGCACAA CAGCCCCGAG GTGGCGGCGA AGACTCAGGG 
ATGGGTGGGA GGAGTTACGC AGAGTCTTCA TCTTCTTCTT CATCTACATC TTCTTCCGAA 
AGTCTGGATT CCTCAGCACC TCTCAGAGAG CATTCTCCTC CGGAAGATGG TGCAATGCTG 
AGTGACAGCT CTGATCTGGC AGACAGCACT TCGGGGGCTC GCACACCTCA CACGTCTGCA 
ATGTCCACCC GGAGCGGGGA GCGCACGCTG CGGTCCCTTG ACCTCTCGAG TGCAGCAACC 
CGCCCTGCGC GCCCCACTCC ACGAGGGAAC GTGACAGAGC ACGCTGGCCT GCTGTCCGGG 
GCACCAACTC TCGGAGTCAC CGGACTTAGC TACACTCGAG AACATGGCTC AGACGCTGGG 
CAGAGGACTT CCAGTGACCA CACAGACCAC GGCTATGTTC CGTCTACTTT CACCAAAGGA 
GAACGGACGC TACTGTCTAT TACAGATAAC ACCTCCTACT CAGAGGCCAG CGAGAGCTCA 
ACCTCTTCCG TTAAGATCTC AGACTCTCCT TCTCAGGCTC AGCCTAAACA GAGTAGCATG 
TCCTCCGACG ATGACGAGCC TGCTCAGTCT TCCACAGAGT CACCGGTTCT GCATACGTCC 
AACCTGCCAA CCTACACGTC CACTGTTAAT ATGCCAAACA CCTTGGTTCT TGATACCGGT 
ACCAAACCTG TTGAGGACCC ATCTGATTCA CGGGTGCCTT CCACTCAACC CTCACCGTCA 
CAACCCCAGC CATTCTCATC AGCCTTACCA TCAACCAGGT CTCCTGGGTC TACCTCTGAG 
ACAACCACAT CCTCACCATC ACCTTCACCC ATCTCCTTAC TGGTGTCAAC TCTTGCCCCA 
TATTCTGTCT CACAAACAAC CTTCCCACAT CCATCTTCTA CCCTAGTCCC TCACAGGCCA 
AGGGAGCCAC GTGTGACTTC AGTCCAAATG TCGACGGCAA TATCAGCCAT TGCATTGATC 
CCCAGCAATC AAACAGCAAA CCCCAAGAAC CAGAGTACCC CACAACAAGA GAAACCCATC 
ACAGAAGCTA AGTCACCAAG CTTGGTGTCT CCGCCCACAG ACTCTACCAA AGCAGTAACA 
GTGAGCCTTC CTCCAGGGGC CCCTTGGTCC CCAGCCTTAA CAGGGTTCTC CACGGGGCCA 
GCCCTTCCAG CCACAAGCAC CAGTTTAGCC CAGATGTCTC CAGCTTTGAC ATCTGCCATG 
CCCCAGACGA CTCACTCTCC TGTGACCAGT CCCAGTACTC TGTCACATGT AGAAGCTTTG 
ACTTCAGGTG CTGTAGTTGT GCACACTACC CCGAAGAAAC CACATCTCCC AACCAATCCT 
GAAATCCTAG TGCCACACAT CTCAACTGAG GGGGCCATCA CCACAGAAGG GAACCGGGAG 
CATACGGATC CTACCACTCA GCCAATCCCT CTGACCACTT CAACCACGTC AGCAGGAGAA 
AGGACCACAG AGCTGGGCCG TGCGGAAGAG TCCAGTCCAT CCCATTTTCT TACACCATCT 
TCACCTCAAA CCACAGATGT TTCTACAGCT GAAATGTTGA CTTCCAGATA TATCACCTTT 
GCTGCTCAGA GCACCTCACA GTCACCAACA GCCTTGCCAC CTCTAACCCC AGTCAACAGC 
TGTACCGTGA ACCCTTGTCT TCATGATGGC AAATGCATTG TGGACCTCAC TGGCCGTGGG 
TATCGATGTG TGTGCCCGCC TGCCTGGCAG GGGGAGAACT GCAGTGTGGA TGTGAACGAA 
TGTCTCTCCA GCCCCTGTCC TCCCCTAGCC ACGTGCAACA ATACTCAGGG ATCGTTCACC 
TGCAGATGCC CAGTCGGGTA TCAGTTGGAG AAAGGAATAT GCAATCTGGT TAGAACCTTT 
GTGACCGAGT TTAAGTTAAA GAAGACTTTT CTCAATACTA CTGCAGAAAA CCATTCCAAC 
ACTCAAGAGC TTGAAAACGA GATCGCTCAA ACACTAAACG TGTGTTTTTC AACATTGCCC 
GGTTATATCC GAACTACAGC CCATGTGTCT AGGGAGCCCA GTACAGTTTT CATCTCACTG 
AAAACCACCT TTGCCCTGGC CTCCAATGTG ACACTGTTTG ACCTGGCAGA CAGGATCCAG 
AAATATGTCA ACTCCTGCAG GTCCTCTGCT GAAGTCTGCC AGCTCTTGGG GTCTCAGAGG 
CGGGTCTTTC GAGCGGGCAG CTTGTGTAAG CGGAAGAGTC CAGAATGTGA CAAAGAGACC 
TCCATCTGCA CCGACCTGGA TGGTGTCGCG CTCTGTCAGT GCAAGTCTGG CTACTTCCAG 
TTCAACAAGA TGGATCACTC TTGCCGAGCA TGTGAAGATG GATATAGGCT TGAAAATGAA 
ACCTGTATGA GTTGCCCATT TGGCCTCGGT GGACTCAACT GTGGAAACCC CTATCAGCTC 
ATCACTGTGG TGATCGCAGC CGCAGGAGGT GGCCTCTTGC TCATCCTGGG CGTGGCACTC 
ATTGTTACCT GTTGCAGAAA GAGTAAAAAT GACATAAGTA AACTCATCTT CAAAAGTGGG 
GACTTCCAAA TGTCCCCATA CACCGATGTC CCCAAGAATC CTCGCTCGCA AGAGTGGGGC 
CGAGAAGCCA TTGAAATGCA CGAGAACGGA AGCACCAAAA ACCTCCTGCA GATGACAGAC 
GTGTACTACT CGCCCACAAA CGTAAGGAAC CCTGAACTTG AACGCAACGG ACTGTACCCA 
GCCTACACTG GACTGCCCGG ATCGAGACAC TCTTGCATCT TCCCGGGACA GTATAACCCA 
TCTTTCATCA GCGACGAGAG CAGGAGGAGA GACTACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522779.1
CDS	181..4122
Translation	MATPRAPRWPPPSLLLLLLLPLLLLPPAAPGARGSLPSPAHRTLLPVAGPLSPPGAGHTAPGPGVATRRGRSGRVPRGVSAALESLPESPSSSRSQRRITPSQTESGTSLGFLERTRELPEEGTVHTQVAGTWVSRQASHPALEPGEPTVLSQKRNSSGQEHSGPPFSWSQSHPPPSDHPSSSGSIKNGNNFTALQNPSVTQTKSMLITDTYTNGVPRTLRSLPVGVDPADETEGFPEHSRLGITSMSVRSSPSVKDSRTNSGLTEHLGDGEGTELSTENGYGLPSIHWQSDAPSFGGRQLASSSEAGDGRAMPLTEAVFRSDPSIGGGESTGRWILTKKKTSTDAAESSALHPEAGGAGGLTQSSHAAQQPRGGGEDSGMGGRSYAESSSSSSSTSSSESLDSSAPLREHSPPEDGAMLSDSSDLADSTSGARTPHTSAMSTRSGERTLRSLDLSSAATRPARPTPRGNVTEHAGLLSGAPTLGVTGLSYTREHGSDAGQRTSSDHTDHGYVPSTFTKGERTLLSITDNTSYSEASESSTSSVKISDSPSQAQPKQSSMSSDDDEPAQSSTESPVLHTSNLPTYTSTVNMPNTLVLDTGTKPVEDPSDSRVPSTQPSPSQPQPFSSALPSTRSPGSTSETTTSSPSPSPISLLVSTLAPYSVSQTTFPHPSSTLVPHRPREPRVTSVQMSTAISAIALIPSNQTANPKNQSTPQQEKPITEAKSPSLVSPPTDSTKAVTVSLPPGAPWSPALTGFSTGPALPATSTSLAQMSPALTSAMPQTTHSPVTSPSTLSHVEALTSGAVVVHTTPKKPHLPTNPEILVPHISTEGAITTEGNREHTDPTTQPIPLTTSTTSAGERTTELGRAEESSPSHFLTPSSPQTTDVSTAEMLTSRYITFAAQSTSQSPTALPPLTPVNSCTVNPCLHDGKCIVDLTGRGYRCVCPPAWQGENCSVDVNECLSSPCPPLATCNNTQGSFTCRCPVGYQLEKGICNLVRTFVTEFKLKKTFLNTTAENHSNTQELENEIAQTLNVCFSTLPGYIRTTAHVSREPSTVFISLKTTFALASNVTLFDLADRIQKYVNSCRSSAEVCQLLGSQRRVFRAGSLCKRKSPECDKETSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGVALIVTCCRKSKNDISKLIFKSGDFQMSPYTDVPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTNVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

Target ORF information:

RefSeq Version	XM_006522716.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522716.1

ORF Insert Sequence:

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCTTGG AGAGCCTTCC TGAGTCACCC AGCAGTTCCA GGTCACAAAG AAGAATCACA 
CCTTCTCAAA CAGAGAGTGG GACATCCCTA GGCTTCCTGG AGAGGACGAG GGAGCTCCCA 
GAAGAAGGGA CTGTGCACAC GCAAGTGGCT GGCACCTGGG TGTCCCGCCA AGCCTCCCAC 
CCTGCTCTGG AACCTGGGGA GCCAACTGTG CTCTCCCAGA AGAGAAATTC ATCCGGCCAG 
GAACACTCCG GGCCACCATT CTCCTGGAGT CAGAGTCACC CTCCTCCCTC AGACCACCCA 
TCGTCTTCTG GAAGTATTAA GAATGGTAAT AATTTCACCG CTCTCCAAAA CCCATCAGTC 
ACCCAGACAA AGAGCATGCT TATCACTGAC ACATACACTA ATGGCGTCCC AAGAACTCTG 
CGGTCTCTGC CCGTTGGTGT GGACCCTGCA GATGAGACAG AGGGTTTCCC TGAGCACTCC 
AGACTTGGTA TAACTTCAAT GTCAGTCCGC TCTTCACCCT CTGTGAAGGA CTCAAGGACA 
AACAGTGGAC TAACTGAGCA TCTGGGAGAT GGAGAAGGCA CTGAGCTGTC TACAGAAAAT 
GGATATGGAC TTCCATCTAT ACACTGGCAA AGTGATGCCC CCTCCTTCGG AGGACGTCAG 
CTTGCCAGCA GCTCAGAAGC GGGCGATGGA AGGGCCATGC CTCTGACTGA GGCTGTGTTT 
AGATCAGACC CCTCCATTGG TGGTGGAGAG AGCACCGGAC GATGGATCCT CACCAAGAAG 
AAAACATCTA CAGATGCGGC AGAAAGCTCA GCTTTGCATC CAGAAGCTGG GGGTGCTGGG 
GGCTTGACTC AGTCCTCACA CGCTGCACAA CAGCCCCGAG GTGGCGGCGA AGACTCAGGG 
ATGGGTGGGA GGAGTTACGC AGAGTCTTCA TCTTCTTCTT CATCTACATC TTCTTCCGAA 
AGTCTGGATT CCTCAGCACC TCTCAGAGAG CATTCTCCTC CGGAAGATGG TGCAATGCTG 
AGTGACAGCT CTGATCTGGC AGACAGCACT TCGGGGGCTC GCACACCTCA CACGTCTGCA 
ATGTCCACCC GGAGCGGGGA GCGCACGCTG CGGTCCCTTG ACCTCTCGAG TGCAGCAACC 
CGCCCTGCGC GCCCCACTCC ACGAGGGAAC GTGACAGAGC ACGCTGGCCT GCTGTCCGGG 
GCACCAACTC TCGGAGTCAC CGGACTTAGC TACACTCGAG AACATGGCTC AGACGCTGGG 
CAGAGGACTT CCAGTGACCA CACAGACCAC GGCTATGTTC CGTCTACTTT CACCAAAGGA 
GAACGGACGC TACTGTCTAT TACAGATAAC ACCTCCTACT CAGAGGCCAG CGAGAGCTCA 
ACCTCTTCCG TTAAGATCTC AGACTCTCCT TCTCAGGCTC AGCCTAAACA GAGTAGCATG 
TCCTCCGACG ATGACGAGCC TGCTCAGTCT TCCACAGAGT CACCGGTTCT GCATACGTCC 
AACCTGCCAA CCTACACGTC CACTGTTAAT ATGCCAAACA CCTTGGTTCT TGATACCGGT 
ACCAAACCTG TTGAGGACCC ATCTGATTCA CGGGTGCCTT CCACTCAACC CTCACCGTCA 
CAACCCCAGC CATTCTCATC AGCCTTACCA TCAACCAGGT CTCCTGGGTC TACCTCTGAG 
ACAACCACAT CCTCACCATC ACCTTCACCC ATCTCCTTAC TGGTGTCAAC TCTTGCCCCA 
TATTCTGTCT CACAAACAAC CTTCCCACAT CCATCTTCTA CCCTAGTCCC TCACAGGCCA 
AGGGAGCCAC GTGTGACTTC AGTCCAAATG TCGACGGCAA TATCAGCCAT TGCATTGATC 
CCCAGCAATC AAACAGCAAA CCCCAAGAAC CAGAGTACCC CACAACAAGA GAAACCCATC 
ACAGAAGCTA AGTCACCAAG CTTGGTGTCT CCGCCCACAG ACTCTACCAA AGCAGTAACA 
GTGAGCCTTC CTCCAGGGGC CCCTTGGTCC CCAGCCTTAA CAGGGTTCTC CACGGGGCCA 
GCCCTTCCAG CCACAAGCAC CAGTTTAGCC CAGATGTCTC CAGCTTTGAC ATCTGCCATG 
CCCCAGACGA CTCACTCTCC TGTGACCAGT CCCAGTACTC TGTCACATGT AGAAGCTTTG 
ACTTCAGGTG CTGTAGTTGT GCACACTACC CCGAAGAAAC CACATCTCCC AACCAATCCT 
GAAATCCTAG TGCCACACAT CTCAACTGAG GGGGCCATCA CCACAGAAGG GAACCGGGAG 
CATACGGATC CTACCACTCA GCCAATCCCT CTGACCACTT CAACCACGTC AGCAGGAGAA 
AGGACCACAG AGCTGGGCCG TGCGGAAGAG TCCAGTCCAT CCCATTTTCT TACACCATCT 
TCACCTCAAA CCACAGATGT TTCTACAGCT GAAATGTTGA CTTCCAGATA TATCACCTTT 
GCTGCTCAGA GCACCTCACA GTCACCAACA GCCTTGCCAC CTCTAACCCC AGTCAACAGC 
TGTACCGTGA ACCCTTGTCT TCATGATGGC AAATGCATTG TGGACCTCAC TGGCCGTGGG 
TATCGATGTG TGTGCCCGCC TGCCTGGCAG GGGGAGAACT GCAGTGTGGA TGTGAACGAA 
TGTCTCTCCA GCCCCTGTCC TCCCCTAGCC ACGTGCAACA ATACTCAGGG ATCGTTCACC 
TGCAGATGCC CAGTCGGGTA TCAGTTGGAG AAAGGAATAT GCAATCTGGT TAGAACCTTT 
GTGACCGAGT TTAAGTTAAA GAAGACTTTT CTCAATACTA CTGCAGAAAA CCATTCCAAC 
ACTCAAGAGC TTGAAAACGA GATCGCTCAA ACACTAAACG TGTGTTTTTC AACATTGCCC 
GGTTATATCC GAACTACAGC CCATGTGTCT AGGGAGCCCA GTACAGTTTT CATCTCACTG 
AAAACCACCT TTGCCCTGGC CTCCAATGTG ACACTGTTTG ACCTGGCAGA CAGGATCCAG 
AAATATGTCA ACTCCTGCAG GTCCTCTGCT GAAGTCTGCC AGCTCTTGGG GTCTCAGAGG 
CGGGTCTTTC GAGCGGGCAG CTTGTGTAAG CGGAAGAGTC CAGAATGTGA CAAAGAGACC 
TCCATCTGCA CCGACCTGGA TGGTGTCGCG CTCTGTCAGT GCAAGTCTGG CTACTTCCAG 
TTCAACAAGA TGGATCACTC TTGCCGAGCA TGTGAAGATG GATATAGGCT TGAAAATGAA 
ACCTGTATGA GTTGCCCATT TGGCCTCGGT GGACTCAACT GTGGAAACCC CTATCAGCTC 
ATCACTGTGG TGATCGCAGC CGCAGGAGGT GGCCTCTTGC TCATCCTGGG CGTGGCACTC 
ATTGTTACCT GTTGCAGAAA GAGTAAAAAT GACATAAGTA AACTCATCTT CAAAAGTGGG 
GACTTCCAAA TGTCCCCATA CACCGATGTC CCCAAGAATC CTCGCTCGCA AGAGTGGGGC 
CGAGAAGCCA TTGAAATGCA CGAGAACGGA AGCACCAAAA ACCTCCTGCA GATGACAGAC 
GTGTACTACT CGCCCACAAA CGTAAGGAAC CCTGAACTTG AACGCAACGG ACTGTACCCA 
GCCTACACTG GACTGCCCGG ATCGAGACAC TCTTGCATCT TCCCGGGACA GTATAACCCA 
TCTTTCATCA GCGACGAGAG CAGGAGGAGA GACTACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heg1 ( XM_006522717.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522780.1 Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu52519
Clone ID Related Accession (Same CDS sequence)	XM_006522717.1 , XM_006522717.2
Accession Version	XM_006522717.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein HEG homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCGCTG CAAGGAACCG TTGGCTGGAA AGTAACAACC CCGAACCCCA TATAGGATGC 
AGTCCCTCTT ATCAGAGTCA AGAGGACCAT TCAGGTTCCA GAAAGGGAGT GACAGCTCAG 
AATGCTAGAA TGAGCCACAG TTCCTCAGAA GGCCCAGAAA ACCCCCCTCT CCTCCCTGAG 
ACAAGTGCTG AGTGGAGCAA CATGGCTTCC TCCCACAGAG CGGACATCGC TGGTCTGCGC 
CGTGGACCCT CCCCTGAGAT CACCACGGCG CCAACTGCCC ACAGCAGCTT GTTGTCCTTG 
GAGAGCCTTC CTGAGTCACC CAGCAGTTCC AGGTCACAAA GAAGAATCAC ACCTTCTCAA 
ACAGAGAGTG GGACATCCCT AGGCTTCCTG GAGAGGACGA GGGAGCTCCC AGAAGAAGGG 
ACTGTGCACA CGCAAGTGGC TGGCACCTGG GTGTCCCGCC AAGCCTCCCA CCCTGCTCTG 
GAACCTGGGG AGCCAACTGT GCTCTCCCAG AAGAGAAATT CATCCGGCCA GGAACACTCC 
GGGCCACCAT TCTCCTGGAG TCAGAGTCAC CCTCCTCCCT CAGACCACCC ATCGTCTTCT 
GGAAGTATTA AGAATGGTAA TAATTTCACC GCTCTCCAAA ACCCATCAGT CACCCAGACA 
AAGAGCATGC TTATCACTGA CACATACACT AATGGCGTCC CAAGAACTCT GCGGTCTCTG 
CCCGTTGGTG TGGACCCTGC AGATGAGACA GAGGGTTTCC CTGAGCACTC CAGACTTGGT 
ATAACTTCAA TGTCAGTCCG CTCTTCACCC TCTGTGAAGG ACTCAAGGAC AAACAGTGGA 
CTAACTGAGC ATCTGGGAGA TGGAGAAGGC ACTGAGCTGT CTACAGAAAA TGGATATGGA 
CTTCCATCTA TACACTGGCA AAGTGATGCC CCCTCCTTCG GAGGACGTCA GCTTGCCAGC 
AGCTCAGAAG CGGGCGATGG AAGGGCCATG CCTCTGACTG AGGCTGTGTT TAGATCAGAC 
CCCTCCATTG GTGGTGGAGA GAGCACCGGA CGATGGATCC TCACCAAGAA GAAAACATCT 
ACAGATGCGG CAGAAAGCTC AGCTTTGCAT CCAGAAGCTG GGGGTGCTGG GGGCTTGACT 
CAGTCCTCAC ACGCTGCACA ACAGCCCCGA GGTGGCGGCG AAGACTCAGG GATGGGTGGG 
AGGAGTTACG CAGAGTCTTC ATCTTCTTCT TCATCTACAT CTTCTTCCGA AAGTCTGGAT 
TCCTCAGCAC CTCTCAGAGA GCATTCTCCT CCGGAAGATG GTGCAATGCT GAGTGACAGC 
TCTGATCTGG CAGACAGCAC TTCGGGGGCT CGCACACCTC ACACGTCTGC AATGTCCACC 
CGGAGCGGGG AGCGCACGCT GCGGTCCCTT GACCTCTCGA GTGCAGCAAC CCGCCCTGCG 
CGCCCCACTC CACGAGGGAA CGTGACAGAG CACGCTGGCC TGCTGTCCGG GGCACCAACT 
CTCGGAGTCA CCGGACTTAG CTACACTCGA GAACATGGCT CAGACGCTGG GCAGAGGACT 
TCCAGTGACC ACACAGACCA CGGCTATGTT CCGTCTACTT TCACCAAAGG AGAACGGACG 
CTACTGTCTA TTACAGATAA CACCTCCTAC TCAGAGGCCA GCGAGAGCTC AACCTCTTCC 
GTTAAGATCT CAGACTCTCC TTCTCAGGCT CAGCCTAAAC AGAGTAGCAT GTCCTCCGAC 
GATGACGAGC CTGCTCAGTC TTCCACAGAG TCACCGGTTC TGCATACGTC CAACCTGCCA 
ACCTACACGT CCACTGTTAA TATGCCAAAC ACCTTGGTTC TTGATACCGG TACCAAACCT 
GTTGAGGACC CATCTGATTC ACGGGTGCCT TCCACTCAAC CCTCACCGTC ACAACCCCAG 
CCATTCTCAT CAGCCTTACC ATCAACCAGG TCTCCTGGGT CTACCTCTGA GACAACCACA 
TCCTCACCAT CACCTTCACC CATCTCCTTA CTGGTGTCAA CTCTTGCCCC ATATTCTGTC 
TCACAAACAA CCTTCCCACA TCCATCTTCT ACCCTAGTCC CTCACAGGCC AAGGGAGCCA 
CGTGTGACTT CAGTCCAAAT GTCGACGGCA ATATCAGCCA TTGCATTGAT CCCCAGCAAT 
CAAACAGCAA ACCCCAAGAA CCAGAGTACC CCACAACAAG AGAAACCCAT CACAGAAGCT 
AAGTCACCAA GCTTGGTGTC TCCGCCCACA GACTCTACCA AAGCAGTAAC AGTGAGCCTT 
CCTCCAGGGG CCCCTTGGTC CCCAGCCTTA ACAGGGTTCT CCACGGGGCC AGCCCTTCCA 
GCCACAAGCA CCAGTTTAGC CCAGATGTCT CCAGCTTTGA CATCTGCCAT GCCCCAGACG 
ACTCACTCTC CTGTGACCAG TCCCAGTACT CTGTCACATG TAGAAGCTTT GACTTCAGGT 
GCTGTAGTTG TGCACACTAC CCCGAAGAAA CCACATCTCC CAACCAATCC TGAAATCCTA 
GTGCCACACA TCTCAACTGA GGGGGCCATC ACCACAGAAG GGAACCGGGA GCATACGGAT 
CCTACCACTC AGCCAATCCC TCTGACCACT TCAACCACGT CAGCAGGAGA AAGGACCACA 
GAGCTGGGCC GTGCGGAAGA GTCCAGTCCA TCCCATTTTC TTACACCATC TTCACCTCAA 
ACCACAGATG TTTCTACAGC TGAAATGTTG ACTTCCAGAT ATATCACCTT TGCTGCTCAG 
AGCACCTCAC AGTCACCAAC AGCCTTGCCA CCTCTAACCC CAGTCAACAG CTGTACCGTG 
AACCCTTGTC TTCATGATGG CAAATGCATT GTGGACCTCA CTGGCCGTGG GTATCGATGT 
GTGTGCCCGC CTGCCTGGCA GGGGGAGAAC TGCAGTGTGG ATGTGAACGA ATGTCTCTCC 
AGCCCCTGTC CTCCCCTAGC CACGTGCAAC AATACTCAGG GATCGTTCAC CTGCAGATGC 
CCAGTCGGGT ATCAGTTGGA GAAAGGAATA TGCAATCTGG TTAGAACCTT TGTGACCGAG 
TTTAAGTTAA AGAAGACTTT TCTCAATACT ACTGCAGAAA ACCATTCCAA CACTCAAGAG 
CTTGAAAACG AGATCGCTCA AACACTAAAC GTGTGTTTTT CAACATTGCC CGGTTATATC 
CGAACTACAG CCCATGTGTC TAGGGAGCCC AGTACAGTTT TCATCTCACT GAAAACCACC 
TTTGCCCTGG CCTCCAATGT GACACTGTTT GACCTGGCAG ACAGGATCCA GAAATATGTC 
AACTCCTGCA GGTCCTCTGC TGAAGTCTGC CAGCTCTTGG GGTCTCAGAG GCGGGTCTTT 
CGAGCGGGCA GCTTGTGTAA GCGGAAGAGT CCAGAATGTG ACAAAGAGAC CTCCATCTGC 
ACCGACCTGG ATGGTGTCGC GCTCTGTCAG TGCAAGTCTG GCTACTTCCA GTTCAACAAG 
ATGGATCACT CTTGCCGAGC ATGTGAAGAT GGATATAGGC TTGAAAATGA AACCTGTATG 
AGTTGCCCAT TTGGCCTCGG TGGACTCAAC TGTGGAAACC CCTATCAGCT CATCACTGTG 
GTGATCGCAG CCGCAGGAGG TGGCCTCTTG CTCATCCTGG GCGTGGCACT CATTGTTACC 
TGTTGCAGCC CACAAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522780.1
CDS	183..4103
Translation	MATPRAPRWPPPSLLLLLLLPLLLLPPAAPGARGSLPSPAHRTLLPVAGPLSPPGAGHTAPGPGVATRRGRSGRVPRGVSAAAARNRWLESNNPEPHIGCSPSYQSQEDHSGSRKGVTAQNARMSHSSSEGPENPPLLPETSAEWSNMASSHRADIAGLRRGPSPEITTAPTAHSSLLSLESLPESPSSSRSQRRITPSQTESGTSLGFLERTRELPEEGTVHTQVAGTWVSRQASHPALEPGEPTVLSQKRNSSGQEHSGPPFSWSQSHPPPSDHPSSSGSIKNGNNFTALQNPSVTQTKSMLITDTYTNGVPRTLRSLPVGVDPADETEGFPEHSRLGITSMSVRSSPSVKDSRTNSGLTEHLGDGEGTELSTENGYGLPSIHWQSDAPSFGGRQLASSSEAGDGRAMPLTEAVFRSDPSIGGGESTGRWILTKKKTSTDAAESSALHPEAGGAGGLTQSSHAAQQPRGGGEDSGMGGRSYAESSSSSSSTSSSESLDSSAPLREHSPPEDGAMLSDSSDLADSTSGARTPHTSAMSTRSGERTLRSLDLSSAATRPARPTPRGNVTEHAGLLSGAPTLGVTGLSYTREHGSDAGQRTSSDHTDHGYVPSTFTKGERTLLSITDNTSYSEASESSTSSVKISDSPSQAQPKQSSMSSDDDEPAQSSTESPVLHTSNLPTYTSTVNMPNTLVLDTGTKPVEDPSDSRVPSTQPSPSQPQPFSSALPSTRSPGSTSETTTSSPSPSPISLLVSTLAPYSVSQTTFPHPSSTLVPHRPREPRVTSVQMSTAISAIALIPSNQTANPKNQSTPQQEKPITEAKSPSLVSPPTDSTKAVTVSLPPGAPWSPALTGFSTGPALPATSTSLAQMSPALTSAMPQTTHSPVTSPSTLSHVEALTSGAVVVHTTPKKPHLPTNPEILVPHISTEGAITTEGNREHTDPTTQPIPLTTSTTSAGERTTELGRAEESSPSHFLTPSSPQTTDVSTAEMLTSRYITFAAQSTSQSPTALPPLTPVNSCTVNPCLHDGKCIVDLTGRGYRCVCPPAWQGENCSVDVNECLSSPCPPLATCNNTQGSFTCRCPVGYQLEKGICNLVRTFVTEFKLKKTFLNTTAENHSNTQELENEIAQTLNVCFSTLPGYIRTTAHVSREPSTVFISLKTTFALASNVTLFDLADRIQKYVNSCRSSAEVCQLLGSQRRVFRAGSLCKRKSPECDKETSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGVALIVTCCSPQT

Target ORF information:

RefSeq Version	XM_006522717.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522717.1

ORF Insert Sequence:

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCGCTG CAAGGAACCG TTGGCTGGAA AGTAACAACC CCGAACCCCA TATAGGATGC 
AGTCCCTCTT ATCAGAGTCA AGAGGACCAT TCAGGTTCCA GAAAGGGAGT GACAGCTCAG 
AATGCTAGAA TGAGCCACAG TTCCTCAGAA GGCCCAGAAA ACCCCCCTCT CCTCCCTGAG 
ACAAGTGCTG AGTGGAGCAA CATGGCTTCC TCCCACAGAG CGGACATCGC TGGTCTGCGC 
CGTGGACCCT CCCCTGAGAT CACCACGGCG CCAACTGCCC ACAGCAGCTT GTTGTCCTTG 
GAGAGCCTTC CTGAGTCACC CAGCAGTTCC AGGTCACAAA GAAGAATCAC ACCTTCTCAA 
ACAGAGAGTG GGACATCCCT AGGCTTCCTG GAGAGGACGA GGGAGCTCCC AGAAGAAGGG 
ACTGTGCACA CGCAAGTGGC TGGCACCTGG GTGTCCCGCC AAGCCTCCCA CCCTGCTCTG 
GAACCTGGGG AGCCAACTGT GCTCTCCCAG AAGAGAAATT CATCCGGCCA GGAACACTCC 
GGGCCACCAT TCTCCTGGAG TCAGAGTCAC CCTCCTCCCT CAGACCACCC ATCGTCTTCT 
GGAAGTATTA AGAATGGTAA TAATTTCACC GCTCTCCAAA ACCCATCAGT CACCCAGACA 
AAGAGCATGC TTATCACTGA CACATACACT AATGGCGTCC CAAGAACTCT GCGGTCTCTG 
CCCGTTGGTG TGGACCCTGC AGATGAGACA GAGGGTTTCC CTGAGCACTC CAGACTTGGT 
ATAACTTCAA TGTCAGTCCG CTCTTCACCC TCTGTGAAGG ACTCAAGGAC AAACAGTGGA 
CTAACTGAGC ATCTGGGAGA TGGAGAAGGC ACTGAGCTGT CTACAGAAAA TGGATATGGA 
CTTCCATCTA TACACTGGCA AAGTGATGCC CCCTCCTTCG GAGGACGTCA GCTTGCCAGC 
AGCTCAGAAG CGGGCGATGG AAGGGCCATG CCTCTGACTG AGGCTGTGTT TAGATCAGAC 
CCCTCCATTG GTGGTGGAGA GAGCACCGGA CGATGGATCC TCACCAAGAA GAAAACATCT 
ACAGATGCGG CAGAAAGCTC AGCTTTGCAT CCAGAAGCTG GGGGTGCTGG GGGCTTGACT 
CAGTCCTCAC ACGCTGCACA ACAGCCCCGA GGTGGCGGCG AAGACTCAGG GATGGGTGGG 
AGGAGTTACG CAGAGTCTTC ATCTTCTTCT TCATCTACAT CTTCTTCCGA AAGTCTGGAT 
TCCTCAGCAC CTCTCAGAGA GCATTCTCCT CCGGAAGATG GTGCAATGCT GAGTGACAGC 
TCTGATCTGG CAGACAGCAC TTCGGGGGCT CGCACACCTC ACACGTCTGC AATGTCCACC 
CGGAGCGGGG AGCGCACGCT GCGGTCCCTT GACCTCTCGA GTGCAGCAAC CCGCCCTGCG 
CGCCCCACTC CACGAGGGAA CGTGACAGAG CACGCTGGCC TGCTGTCCGG GGCACCAACT 
CTCGGAGTCA CCGGACTTAG CTACACTCGA GAACATGGCT CAGACGCTGG GCAGAGGACT 
TCCAGTGACC ACACAGACCA CGGCTATGTT CCGTCTACTT TCACCAAAGG AGAACGGACG 
CTACTGTCTA TTACAGATAA CACCTCCTAC TCAGAGGCCA GCGAGAGCTC AACCTCTTCC 
GTTAAGATCT CAGACTCTCC TTCTCAGGCT CAGCCTAAAC AGAGTAGCAT GTCCTCCGAC 
GATGACGAGC CTGCTCAGTC TTCCACAGAG TCACCGGTTC TGCATACGTC CAACCTGCCA 
ACCTACACGT CCACTGTTAA TATGCCAAAC ACCTTGGTTC TTGATACCGG TACCAAACCT 
GTTGAGGACC CATCTGATTC ACGGGTGCCT TCCACTCAAC CCTCACCGTC ACAACCCCAG 
CCATTCTCAT CAGCCTTACC ATCAACCAGG TCTCCTGGGT CTACCTCTGA GACAACCACA 
TCCTCACCAT CACCTTCACC CATCTCCTTA CTGGTGTCAA CTCTTGCCCC ATATTCTGTC 
TCACAAACAA CCTTCCCACA TCCATCTTCT ACCCTAGTCC CTCACAGGCC AAGGGAGCCA 
CGTGTGACTT CAGTCCAAAT GTCGACGGCA ATATCAGCCA TTGCATTGAT CCCCAGCAAT 
CAAACAGCAA ACCCCAAGAA CCAGAGTACC CCACAACAAG AGAAACCCAT CACAGAAGCT 
AAGTCACCAA GCTTGGTGTC TCCGCCCACA GACTCTACCA AAGCAGTAAC AGTGAGCCTT 
CCTCCAGGGG CCCCTTGGTC CCCAGCCTTA ACAGGGTTCT CCACGGGGCC AGCCCTTCCA 
GCCACAAGCA CCAGTTTAGC CCAGATGTCT CCAGCTTTGA CATCTGCCAT GCCCCAGACG 
ACTCACTCTC CTGTGACCAG TCCCAGTACT CTGTCACATG TAGAAGCTTT GACTTCAGGT 
GCTGTAGTTG TGCACACTAC CCCGAAGAAA CCACATCTCC CAACCAATCC TGAAATCCTA 
GTGCCACACA TCTCAACTGA GGGGGCCATC ACCACAGAAG GGAACCGGGA GCATACGGAT 
CCTACCACTC AGCCAATCCC TCTGACCACT TCAACCACGT CAGCAGGAGA AAGGACCACA 
GAGCTGGGCC GTGCGGAAGA GTCCAGTCCA TCCCATTTTC TTACACCATC TTCACCTCAA 
ACCACAGATG TTTCTACAGC TGAAATGTTG ACTTCCAGAT ATATCACCTT TGCTGCTCAG 
AGCACCTCAC AGTCACCAAC AGCCTTGCCA CCTCTAACCC CAGTCAACAG CTGTACCGTG 
AACCCTTGTC TTCATGATGG CAAATGCATT GTGGACCTCA CTGGCCGTGG GTATCGATGT 
GTGTGCCCGC CTGCCTGGCA GGGGGAGAAC TGCAGTGTGG ATGTGAACGA ATGTCTCTCC 
AGCCCCTGTC CTCCCCTAGC CACGTGCAAC AATACTCAGG GATCGTTCAC CTGCAGATGC 
CCAGTCGGGT ATCAGTTGGA GAAAGGAATA TGCAATCTGG TTAGAACCTT TGTGACCGAG 
TTTAAGTTAA AGAAGACTTT TCTCAATACT ACTGCAGAAA ACCATTCCAA CACTCAAGAG 
CTTGAAAACG AGATCGCTCA AACACTAAAC GTGTGTTTTT CAACATTGCC CGGTTATATC 
CGAACTACAG CCCATGTGTC TAGGGAGCCC AGTACAGTTT TCATCTCACT GAAAACCACC 
TTTGCCCTGG CCTCCAATGT GACACTGTTT GACCTGGCAG ACAGGATCCA GAAATATGTC 
AACTCCTGCA GGTCCTCTGC TGAAGTCTGC CAGCTCTTGG GGTCTCAGAG GCGGGTCTTT 
CGAGCGGGCA GCTTGTGTAA GCGGAAGAGT CCAGAATGTG ACAAAGAGAC CTCCATCTGC 
ACCGACCTGG ATGGTGTCGC GCTCTGTCAG TGCAAGTCTG GCTACTTCCA GTTCAACAAG 
ATGGATCACT CTTGCCGAGC ATGTGAAGAT GGATATAGGC TTGAAAATGA AACCTGTATG 
AGTTGCCCAT TTGGCCTCGG TGGACTCAAC TGTGGAAACC CCTATCAGCT CATCACTGTG 
GTGATCGCAG CCGCAGGAGG TGGCCTCTTG CTCATCCTGG GCGTGGCACT CATTGTTACC 
TGTTGCAGCC CACAAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heg1 ( XM_006522718.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522781.1 Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu52520
Clone ID Related Accession (Same CDS sequence)	XM_006522718.2
Accession Version	XM_006522718.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein HEG homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522718.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCGCTG CAAGGAACCG TTGGCTGGAA AGTAACAACC CCGAACCCCA TATAGGATGC 
AGTCCCTCTT ATCAGAGTCA AGAGGACCAT TCAGGTTCCA GAAAGGGAGT GACAGCTCAG 
AATGCTAGAA TGAGCCACAG TTCCTCAGAA GGCCCAGAAA ACCCCCCTCT CCTCCCTGAG 
ACAAGTGCTG AGTGGAGCAA CATGGCTTCC TCCCACAGAG CGGACATCGC TGGTCTGCGC 
CGTGGACCCT CCCCTGAGAT CACCACGGCG CCAACTGCCC ACAGCAGCTT GTTGTCCTTG 
GAGAGCCTTC CTGAGTCACC CAGCAGTTCC AGGTCACAAA GAAGAATCAC ACCTTCTCAA 
ACAGAGAGTG GGACATCCCT AGGCTTCCTG GAGAGGACGA GGGAGCTCCC AGAAGAAGGG 
ACTGTGCACA CGCAAGTGGC TGGCACCTGG GTGTCCCGCC AAGCCTCCCA CCCTGCTCTG 
GAACCTGGGG AGCCAACTGT GCTCTCCCAG AAGAGAAATT CATCCGGCCA GGAACACTCC 
GGGCCACCAT TCTCCTGGAG TCAGAGTCAC CCTCCTCCCT CAGACCACCC ATCGTCTCCG 
GAAGATGGTG CAATGCTGAG TGACAGCTCT GATCTGGCAG ACAGCACTTC GGGGGCTCGC 
ACACCTCACA CGTCTGCAAT GTCCACCCGG AGCGGGGAGC GCACGCTGCG GTCCCTTGAC 
CTCTCGAGTG CAGCAACCCG CCCTGCGCGC CCCACTCCAC GAGGGAACGT GACAGAGCAC 
GCTGGCCTGC TGTCCGGGGC ACCAACTCTC GGAGTCACCG GACTTAGCTA CACTCGAGAA 
CATGGCTCAG ACGCTGGGCA GAGGACTTCC AGTGACCACA CAGACCACGG CTATGTTCCG 
TCTACTTTCA CCAAAGGAGA ACGGACGCTA CTGTCTATTA CAGATAACAC CTCCTACTCA 
GAGGCCAGCG AGAGCTCAAC CTCTTCCGTT AAGATCTCAG ACTCTCCTTC TCAGGCTCAG 
CCTAAACAGA GTAGCATGTC CTCCGACGAT GACGAGCCTG CTCAGTCTTC CACAGAGTCA 
CCGGTTCTGC ATACGTCCAA CCTGCCAACC TACACGTCCA CTGTTAATAT GCCAAACACC 
TTGGTTCTTG ATACCGGTAC CAAACCTGTT GAGGACCCAT CTGATTCACG GGTGCCTTCC 
ACTCAACCCT CACCGTCACA ACCCCAGCCA TTCTCATCAG CCTTACCATC AACCAGGTCT 
CCTGGGTCTA CCTCTGAGAC AACCACATCC TCACCATCAC CTTCACCCAT CTCCTTACTG 
GTGTCAACTC TTGCCCCATA TTCTGTCTCA CAAACAACCT TCCCACATCC ATCTTCTACC 
CTAGTCCCTC ACAGGCCAAG GGAGCCACGT GTGACTTCAG TCCAAATGTC GACGGCAATA 
TCAGCCATTG CATTGATCCC CAGCAATCAA ACAGCAAACC CCAAGAACCA GAGTACCCCA 
CAACAAGAGA AACCCATCAC AGAAGCTAAG TCACCAAGCT TGGTGTCTCC GCCCACAGAC 
TCTACCAAAG CAGTAACAGT GAGCCTTCCT CCAGGGGCCC CTTGGTCCCC AGCCTTAACA 
GGGTTCTCCA CGGGGCCAGC CCTTCCAGCC ACAAGCACCA GTTTAGCCCA GATGTCTCCA 
GCTTTGACAT CTGCCATGCC CCAGACGACT CACTCTCCTG TGACCAGTCC CAGTACTCTG 
TCACATGTAG AAGCTTTGAC TTCAGGTGCT GTAGTTGTGC ACACTACCCC GAAGAAACCA 
CATCTCCCAA CCAATCCTGA AATCCTAGTG CCACACATCT CAACTGAGGG GGCCATCACC 
ACAGAAGGGA ACCGGGAGCA TACGGATCCT ACCACTCAGC CAATCCCTCT GACCACTTCA 
ACCACGTCAG CAGGAGAAAG GACCACAGAG CTGGGCCGTG CGGAAGAGTC CAGTCCATCC 
CATTTTCTTA CACCATCTTC ACCTCAAACC ACAGATGTTT CTACAGCTGA AATGTTGACT 
TCCAGATATA TCACCTTTGC TGCTCAGAGC ACCTCACAGT CACCAACAGC CTTGCCACCT 
CTAACCCCAG TCAACAGCTG TACCGTGAAC CCTTGTCTTC ATGATGGCAA ATGCATTGTG 
GACCTCACTG GCCGTGGGTA TCGATGTGTG TGCCCGCCTG CCTGGCAGGG GGAGAACTGC 
AGTGTGGATG TGAACGAATG TCTCTCCAGC CCCTGTCCTC CCCTAGCCAC GTGCAACAAT 
ACTCAGGGAT CGTTCACCTG CAGATGCCCA GTCGGGTATC AGTTGGAGAA AGGAATATGC 
AATCTGGTTA GAACCTTTGT GACCGAGTTT AAGTTAAAGA AGACTTTTCT CAATACTACT 
GCAGAAAACC ATTCCAACAC TCAAGAGCTT GAAAACGAGA TCGCTCAAAC ACTAAACGTG 
TGTTTTTCAA CATTGCCCGG TTATATCCGA ACTACAGCCC ATGTGTCTAG GGAGCCCAGT 
ACAGTTTTCA TCTCACTGAA AACCACCTTT GCCCTGGCCT CCAATGTGAC ACTGTTTGAC 
CTGGCAGACA GGATCCAGAA ATATGTCAAC TCCTGCAGGT CCTCTGCTGA AGTCTGCCAG 
CTCTTGGGGT CTCAGAGGCG GGTCTTTCGA GCGGGCAGCT TGTGTAAGCG GAAGAGTCCA 
GAATGTGACA AAGAGACCTC CATCTGCACC GACCTGGATG GTGTCGCGCT CTGTCAGTGC 
AAGTCTGGCT ACTTCCAGTT CAACAAGATG GATCACTCTT GCCGAGCATG TGAAGATGGA 
TATAGGCTTG AAAATGAAAC CTGTATGAGT TGCCCATTTG GCCTCGGTGG ACTCAACTGT 
GGAAACCCCT ATCAGCTCAT CACTGTGGTG ATCGCAGCCG CAGGAGGTGG CCTCTTGCTC 
ATCCTGGGCG TGGCACTCAT TGTTACCTGT TGCAGAAAGA GTAAAAATGA CATAAGTAAA 
CTCATCTTCA AAAGTGGGGA CTTCCAAATG TCCCCATACA CCGATGTCCC CAAGAATCCT 
CGCTCGCAAG AGTGGGGCCG AGAAGCCATT GAAATGCACG AGAACGGAAG CACCAAAAAC 
CTCCTGCAGA TGACAGACGT GTACTACTCG CCCACAAACG TAAGGAACCC TGAACTTGAA 
CGCAACGGAC TGTACCCAGC CTACACTGGA CTGCCCGGAT CGAGACACTC TTGCATCTTC 
CCGGGACAGT ATAACCCATC TTTCATCAGC GACGAGAGCA GGAGGAGAGA CTACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522781.1
CDS	181..3720
Translation	MATPRAPRWPPPSLLLLLLLPLLLLPPAAPGARGSLPSPAHRTLLPVAGPLSPPGAGHTAPGPGVATRRGRSGRVPRGVSAAAARNRWLESNNPEPHIGCSPSYQSQEDHSGSRKGVTAQNARMSHSSSEGPENPPLLPETSAEWSNMASSHRADIAGLRRGPSPEITTAPTAHSSLLSLESLPESPSSSRSQRRITPSQTESGTSLGFLERTRELPEEGTVHTQVAGTWVSRQASHPALEPGEPTVLSQKRNSSGQEHSGPPFSWSQSHPPPSDHPSSPEDGAMLSDSSDLADSTSGARTPHTSAMSTRSGERTLRSLDLSSAATRPARPTPRGNVTEHAGLLSGAPTLGVTGLSYTREHGSDAGQRTSSDHTDHGYVPSTFTKGERTLLSITDNTSYSEASESSTSSVKISDSPSQAQPKQSSMSSDDDEPAQSSTESPVLHTSNLPTYTSTVNMPNTLVLDTGTKPVEDPSDSRVPSTQPSPSQPQPFSSALPSTRSPGSTSETTTSSPSPSPISLLVSTLAPYSVSQTTFPHPSSTLVPHRPREPRVTSVQMSTAISAIALIPSNQTANPKNQSTPQQEKPITEAKSPSLVSPPTDSTKAVTVSLPPGAPWSPALTGFSTGPALPATSTSLAQMSPALTSAMPQTTHSPVTSPSTLSHVEALTSGAVVVHTTPKKPHLPTNPEILVPHISTEGAITTEGNREHTDPTTQPIPLTTSTTSAGERTTELGRAEESSPSHFLTPSSPQTTDVSTAEMLTSRYITFAAQSTSQSPTALPPLTPVNSCTVNPCLHDGKCIVDLTGRGYRCVCPPAWQGENCSVDVNECLSSPCPPLATCNNTQGSFTCRCPVGYQLEKGICNLVRTFVTEFKLKKTFLNTTAENHSNTQELENEIAQTLNVCFSTLPGYIRTTAHVSREPSTVFISLKTTFALASNVTLFDLADRIQKYVNSCRSSAEVCQLLGSQRRVFRAGSLCKRKSPECDKETSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGVALIVTCCRKSKNDISKLIFKSGDFQMSPYTDVPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTNVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

Target ORF information:

RefSeq Version	XM_006522718.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus heart development protein with EGF-like domains 1 (Heg1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522718.2

ORF Insert Sequence:

ATGGCCACGC CGCGCGCCCC GCGGTGGCCG CCACCGTCGC TGCTGCTGCT GCTGCTGCTA 
CCGCTGCTGC TGCTGCCGCC CGCTGCCCCC GGGGCGCGGG GCTCCCTTCC CTCCCCGGCC 
CATCGAACAC TCCTCCCGGT CGCGGGACCG CTTTCGCCGC CTGGCGCCGG ACATACGGCC 
CCCGGACCCG GCGTCGCGAC CCGGCGGGGA CGTTCCGGCC GTGTCCCCAG AGGCGTGAGC 
GCGGCCGCTG CAAGGAACCG TTGGCTGGAA AGTAACAACC CCGAACCCCA TATAGGATGC 
AGTCCCTCTT ATCAGAGTCA AGAGGACCAT TCAGGTTCCA GAAAGGGAGT GACAGCTCAG 
AATGCTAGAA TGAGCCACAG TTCCTCAGAA GGCCCAGAAA ACCCCCCTCT CCTCCCTGAG 
ACAAGTGCTG AGTGGAGCAA CATGGCTTCC TCCCACAGAG CGGACATCGC TGGTCTGCGC 
CGTGGACCCT CCCCTGAGAT CACCACGGCG CCAACTGCCC ACAGCAGCTT GTTGTCCTTG 
GAGAGCCTTC CTGAGTCACC CAGCAGTTCC AGGTCACAAA GAAGAATCAC ACCTTCTCAA 
ACAGAGAGTG GGACATCCCT AGGCTTCCTG GAGAGGACGA GGGAGCTCCC AGAAGAAGGG 
ACTGTGCACA CGCAAGTGGC TGGCACCTGG GTGTCCCGCC AAGCCTCCCA CCCTGCTCTG 
GAACCTGGGG AGCCAACTGT GCTCTCCCAG AAGAGAAATT CATCCGGCCA GGAACACTCC 
GGGCCACCAT TCTCCTGGAG TCAGAGTCAC CCTCCTCCCT CAGACCACCC ATCGTCTCCG 
GAAGATGGTG CAATGCTGAG TGACAGCTCT GATCTGGCAG ACAGCACTTC GGGGGCTCGC 
ACACCTCACA CGTCTGCAAT GTCCACCCGG AGCGGGGAGC GCACGCTGCG GTCCCTTGAC 
CTCTCGAGTG CAGCAACCCG CCCTGCGCGC CCCACTCCAC GAGGGAACGT GACAGAGCAC 
GCTGGCCTGC TGTCCGGGGC ACCAACTCTC GGAGTCACCG GACTTAGCTA CACTCGAGAA 
CATGGCTCAG ACGCTGGGCA GAGGACTTCC AGTGACCACA CAGACCACGG CTATGTTCCG 
TCTACTTTCA CCAAAGGAGA ACGGACGCTA CTGTCTATTA CAGATAACAC CTCCTACTCA 
GAGGCCAGCG AGAGCTCAAC CTCTTCCGTT AAGATCTCAG ACTCTCCTTC TCAGGCTCAG 
CCTAAACAGA GTAGCATGTC CTCCGACGAT GACGAGCCTG CTCAGTCTTC CACAGAGTCA 
CCGGTTCTGC ATACGTCCAA CCTGCCAACC TACACGTCCA CTGTTAATAT GCCAAACACC 
TTGGTTCTTG ATACCGGTAC CAAACCTGTT GAGGACCCAT CTGATTCACG GGTGCCTTCC 
ACTCAACCCT CACCGTCACA ACCCCAGCCA TTCTCATCAG CCTTACCATC AACCAGGTCT 
CCTGGGTCTA CCTCTGAGAC AACCACATCC TCACCATCAC CTTCACCCAT CTCCTTACTG 
GTGTCAACTC TTGCCCCATA TTCTGTCTCA CAAACAACCT TCCCACATCC ATCTTCTACC 
CTAGTCCCTC ACAGGCCAAG GGAGCCACGT GTGACTTCAG TCCAAATGTC GACGGCAATA 
TCAGCCATTG CATTGATCCC CAGCAATCAA ACAGCAAACC CCAAGAACCA GAGTACCCCA 
CAACAAGAGA AACCCATCAC AGAAGCTAAG TCACCAAGCT TGGTGTCTCC GCCCACAGAC 
TCTACCAAAG CAGTAACAGT GAGCCTTCCT CCAGGGGCCC CTTGGTCCCC AGCCTTAACA 
GGGTTCTCCA CGGGGCCAGC CCTTCCAGCC ACAAGCACCA GTTTAGCCCA GATGTCTCCA 
GCTTTGACAT CTGCCATGCC CCAGACGACT CACTCTCCTG TGACCAGTCC CAGTACTCTG 
TCACATGTAG AAGCTTTGAC TTCAGGTGCT GTAGTTGTGC ACACTACCCC GAAGAAACCA 
CATCTCCCAA CCAATCCTGA AATCCTAGTG CCACACATCT CAACTGAGGG GGCCATCACC 
ACAGAAGGGA ACCGGGAGCA TACGGATCCT ACCACTCAGC CAATCCCTCT GACCACTTCA 
ACCACGTCAG CAGGAGAAAG GACCACAGAG CTGGGCCGTG CGGAAGAGTC CAGTCCATCC 
CATTTTCTTA CACCATCTTC ACCTCAAACC ACAGATGTTT CTACAGCTGA AATGTTGACT 
TCCAGATATA TCACCTTTGC TGCTCAGAGC ACCTCACAGT CACCAACAGC CTTGCCACCT 
CTAACCCCAG TCAACAGCTG TACCGTGAAC CCTTGTCTTC ATGATGGCAA ATGCATTGTG 
GACCTCACTG GCCGTGGGTA TCGATGTGTG TGCCCGCCTG CCTGGCAGGG GGAGAACTGC 
AGTGTGGATG TGAACGAATG TCTCTCCAGC CCCTGTCCTC CCCTAGCCAC GTGCAACAAT 
ACTCAGGGAT CGTTCACCTG CAGATGCCCA GTCGGGTATC AGTTGGAGAA AGGAATATGC 
AATCTGGTTA GAACCTTTGT GACCGAGTTT AAGTTAAAGA AGACTTTTCT CAATACTACT 
GCAGAAAACC ATTCCAACAC TCAAGAGCTT GAAAACGAGA TCGCTCAAAC ACTAAACGTG 
TGTTTTTCAA CATTGCCCGG TTATATCCGA ACTACAGCCC ATGTGTCTAG GGAGCCCAGT 
ACAGTTTTCA TCTCACTGAA AACCACCTTT GCCCTGGCCT CCAATGTGAC ACTGTTTGAC 
CTGGCAGACA GGATCCAGAA ATATGTCAAC TCCTGCAGGT CCTCTGCTGA AGTCTGCCAG 
CTCTTGGGGT CTCAGAGGCG GGTCTTTCGA GCGGGCAGCT TGTGTAAGCG GAAGAGTCCA 
GAATGTGACA AAGAGACCTC CATCTGCACC GACCTGGATG GTGTCGCGCT CTGTCAGTGC 
AAGTCTGGCT ACTTCCAGTT CAACAAGATG GATCACTCTT GCCGAGCATG TGAAGATGGA 
TATAGGCTTG AAAATGAAAC CTGTATGAGT TGCCCATTTG GCCTCGGTGG ACTCAACTGT 
GGAAACCCCT ATCAGCTCAT CACTGTGGTG ATCGCAGCCG CAGGAGGTGG CCTCTTGCTC 
ATCCTGGGCG TGGCACTCAT TGTTACCTGT TGCAGAAAGA GTAAAAATGA CATAAGTAAA 
CTCATCTTCA AAAGTGGGGA CTTCCAAATG TCCCCATACA CCGATGTCCC CAAGAATCCT 
CGCTCGCAAG AGTGGGGCCG AGAAGCCATT GAAATGCACG AGAACGGAAG CACCAAAAAC 
CTCCTGCAGA TGACAGACGT GTACTACTCG CCCACAAACG TAAGGAACCC TGAACTTGAA 
CGCAACGGAC TGTACCCAGC CTACACTGGA CTGCCCGGAT CGAGACACTC TTGCATCTTC 
CCGGGACAGT ATAACCCATC TTTCATCAGC GACGAGAGCA GGAGGAGAGA CTACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heart of glass anchors Rasip1 at endothelial cell-cell junctions to support vascular integrity.
eLife5e11394(2016 Jan)
de Kreuk BJ,Gingras AR,Knight JD,Liu JJ,Gingras AC,Ginsberg MH

Dynamic regulation of the cerebral cavernous malformation pathway controls vascular stability and growth.
Developmental cell23(2)342-55(2012 Aug)
Zheng X,Xu C,Smith AO,Stratman AN,Zou Z,Kleaveland B,Yuan L,Didiku C,Sen A,Liu X,Skuli N,Zaslavsky A,Chen M,Cheng L,Davis GE,Kahn ML

Gene expression analysis of the embryonic subplate.
Cerebral cortex (New York, N.Y. : 1991)22(6)1343-59(2012 Jun)
Oeschger FM,Wang WZ,Lee S,Garc?a-Moreno F,Goffinet AM,Arbon?s ML,Rakic S,Moln?r Z

Regulation of cardiovascular development and integrity by the heart of glass-cerebral cavernous malformation protein pathway.
Nature medicine15(2)169-76(2009 Feb)
Kleaveland B,Zheng X,Liu JJ,Blum Y,Tung JJ,Zou Z,Sweeney SM,Chen M,Guo L,Lu MM,Zhou D,Kitajewski J,Affolter M,Ginsberg MH,Kahn ML

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Heg1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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