Bicd2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bicd2
Entrez Gene ID	76895
Full Name	BICD cargo adaptor 2
General protein information	Preferred Names BICD cargo adaptor 2 Names protein bicaudal D homolog 2 bic-D 2 bicaudal D homolog 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A5

mRNA and Protein(s)

mRNA	Protein	Name
XM_011244429.2	XP_011242731.1	protein bicaudal D homolog 2 isoform X1
XM_011244429.3	XP_011242731.1	protein bicaudal D homolog 2 isoform X1
NM_029791.4	NP_084067.1	protein bicaudal D homolog 2 isoform 2
NM_001039180.2	NP_001034269.1	protein bicaudal D homolog 2 isoform 3
NM_001039179.2	NP_001034268.1	protein bicaudal D homolog 2 isoform 1

Pathways from BioSystems

Homologies

	BICD2	XP_001103763.1
Canis lupus familiaris (dog)	BICD2	XP_541330.2
Mus musculus (house mouse)	Bicd2	NP_001034268.1
Caenorhabditis elegans (roundworm)	bicd-1	NP_501001.1
Bos taurus (cattle)	BICD2	NP_001193404.1
Rattus norvegicus (Norway rat)	Bicd2	NP_001028846.1
Gallus gallus (chicken)	BICD2	XP_004944615.1
Danio rerio (zebrafish)	LOC557383	XP_005155947.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010206	XP_554113.3
Homo sapiens (human)	BICD2	NP_001003800.1
Pan troglodytes (chimpanzee)	BICD2	XP_520695.2
Drosophila melanogaster (fruit fly)	BicD	NP_001260531.1
Xenopus tropicalis (tropical clawed frog)	bicd2	XP_004914244.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential effects of the dynein-regulatory factor Lissencephaly-1 on processive dynein-dynactin motility.
Gutierrez PA, Ackermann BE, Vershinin M, McKenney RJ
The Journal of biological chemistry292(29)12245-12255(2017 07)

Reconstitution of the targeting of Rab6A to the Golgi apparatus in semi-intact HeLa cells: A role of BICD2 in stabilizing Rab6A on Golgi membranes and a concerted role of Rab6A/BICD2 interactions in Golgi-to-ER retrograde transport.
Matsuto M, Kano F, Murata M
Biochimica et biophysica acta1853(10 Pt A)2592-609(2015 Oct)

A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region.
Schroeder CM, Ostrem JM, Hertz NT, Vale RD
eLife3e03351(2014 Oct)

A role for Bicaudal-D2 in radial cerebellar granule cell migration.
Jaarsma D, van den Berg R, Wulf PS, van Erp S, Keijzer N, Schlager MA, de Graaff E, De Zeeuw CI, Pasterkamp RJ, Akhmanova A, Hoogenraad CC
Nature communications53411(2014 Mar)

BICD2, dynactin, and LIS1 cooperate in regulating dynein recruitment to cellular structures.
Splinter D, Razafsky DS, Schlager MA, Serra-Marques A, Grigoriev I, Demmers J, Keijzer N, Jiang K, Poser I, Hyman AA, Hoogenraad CC, King SJ, Akhmanova A
Molecular biology of the cell23(21)4226-41(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that binding of Lis1 to DDB complex does not strongly disrupt processive motility along microtubules; motile DDB complex binds up to two Lis1 dimers; mutational analysis suggested that Lis1 binds directly to the dynein motor domains during DDB complex movement. (Lis1 = lissencephaly 1 protein; DDB complex = dynein-dynactin-BicD2 complex; BicD2 = bicaudal D homolog 2 protein)
Title: Differential effects of the dynein-regulatory factor Lissencephaly-1 on processive dynein-dynactin motility.

several analyses of vesicular transport demonstrated that Rab6A and BICD2 play crucial roles in Golgi tubule fusion with the endoplasmic reticulum (ER) in brefeldin A (BFA)-treated cells
Title: Reconstitution of the targeting of Rab6A to the Golgi apparatus in semi-intact HeLa cells: A role of BICD2 in stabilizing Rab6A on Golgi membranes and a concerted role of Rab6A/BICD2 interactions in Golgi-to-ER retrograde transport.

Data indicate that Bicaudal-D2 (BICD2)-deficient mice develop disrupted laminar organization of cerebral cortex and the cerebellum, pointing to impaired radial neuronal migration.
Title: A role for Bicaudal-D2 in radial cerebellar granule cell migration.

LIS1 is required for dynein-mediated transport induced by membrane tethering of BICD2-N and LIS1 contributes to dynein accumulation at microtubule plus ends and BICD2-positive cellular structures.
Title: BICD2, dynactin, and LIS1 cooperate in regulating dynein recruitment to cellular structures.

BICD2-N transgene increased lifespan in 'low copy' SOD1-G93A ALS transgenic mice.
Title: A novel mouse model with impaired dynein/dynactin function develops amyotrophic lateral sclerosis (ALS)-like features in motor neurons and improves lifespan in SOD1-ALS mice.

The following Bicd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bicd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00504	NM_001039179.2 Latest version!	Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$496.30 $709.00
OMu00506	NM_001039180.2 Latest version!	Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$440.30 $629.00
OMu07477	NM_029791.4 Latest version!	Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$482.30 $689.00
OMu71587	XM_011244429.3 Latest version!	Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu71587	XM_011244429.2	Mus musculus bicaudal D homolog 2 (Drosophila) (Bicd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bicd2 ( NM_001039179.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034268.1 Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00504
Clone ID Related Accession (Same CDS sequence)	NM_001039179.2
Accession Version	NM_001039179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein bicaudal D homolog 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF538535.1, AK122348.1 and BP775855.1. On Oct 22, 2013 this sequence version replaced NM_001039179.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122348.1, SRR1660813.226594.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCGC CGTCGGAGGA GGAGGAGTAT GCGCGTCTGG TGATGGAGGC GCAGCCGGAG 
TGGCTGCGCG CCGAGGTGAA GCGGCTGTCC CACGAGCTGG CCGAGACCAC GCGTGAGAAG 
ATCCAGGCGG CCGAGTATGG ACTGGCGGTG CTGGAGGAGA AGCACCAGCT CAAGCTGCAG 
TTCGAGGAGC TCGAGGTGGA CTATGAGGCC ATCCGCAGCG AGATGGAGCA GCTCAAAGAG 
GCATTTGGCC AGGCACATAC AAACCACAAG AAGGTGGCTG CTGATGGTGA GAGCCGGGAG 
GAGAGCCTGA TCCAGGAGTC GGCCTCCAAG GAGCAGTACT ACGTGCGGAA GGTGCTGGAG 
CTGCAGACAG AGCTGAAACA GCTGCGCAAC GTCCTCACCA ACACCCAGTC TGAGAATGAG 
CGCCTCACGT CTGTGGCCCA GGAGCTAAAA GAGATCAACC AGAATGTGGA GATCCAGCGT 
GGTCGCCTGC GGGATGACAT CAAGGAGTAC AAGTTCCGGG AGGCCCGTCT ACTTCAGGAC 
TACTCAGAGC TGGAGGAGGA GAACATCAGC CTGCAGAAAC AAGTGTCTGT GCTCAGGCAG 
AACCAGGTGG AGTTTGAGGG CCTCAAGCAT GAAATCAAGC GCCTGGAGGA GGAGACAGAG 
TACCTCAACA GCCAGCTAGA GGATGCCATC CGGCTTAAGG AGATCTCTGA ACGGCAGTTG 
GAGGAGGCGC TGGAGACACT GAAGACAGAG CGGGAGCAGA AGAACAACCT GCGCAAGGAG 
TTGTCGCACT ACATGAGCAT CAACGATTCC TTCTATACCA GCCACCTGCA GGTCTCCTTA 
GATGGCCTCA AGTTCAGTGA TGATACTGTC ACCGCAGAGC CCAACAATGA CGCCGAAGCC 
CTGGTCAATG GCTTTGAGCA CAGTGGCTTG GTCAAATCAT CCTTGGACAA CAAGACATCC 
ACACCCAGGA AGGATGGCCT AGCTCCACCC TCCCCCAGCC TCGTCTCTGA CCTGCTCAGT 
GAGCTCCACA TATCTGAGAT CCAGAAGCTG AAACAGCAGC TGGTGCAGAT GGAGCGGGAG 
AAGGTGGGCC TGCTGGCAAC ACTGCAGGAC ACACAGAAGC AGCTGGAGCA GGCTCGGGGA 
ACCCTCTCTG AGCAGCATGA GAAGGTGAAT CGTCTCACAG AGAACCTTAG TGCCCTCCGG 
CGCCTGCAGG CTGGCAAGGA GCGACAGACC TCGTTGGATA ATGAGAAGGA CCGTGACAGC 
CACGAAGACG GTGACTACTA TGAGGTGGAC ATCAATGGGC CTGAGATCCT GGCCTGCAAG 
TACCACGTGG CTGTGGCTGA GGCTGGCGAG CTCCGGGAGC AGCTCAAGGC GTTGCGCAGC 
ACACACGAAG CTCGCGAAGC CCAGCACGCA GAAGAAAAGG GCCGGTATGA GGCTGAGGGC 
CAGGCACTCA CTGAGAAGAT CTCTCTGCTG GAGAAGGCTA GCCACCAGGA CCGAGAGCTG 
CTGGCCCATC TGGAAAAGGA GCTGAAGAAG GTGAGCGATG TGGCTGGTGA GACCCAGGGC 
AGCCTGAATG TGGCTCAGGA TGAGCTGGTG ACCTTCAGCG AGGAGCTGGC CAACCTCTAC 
CACCATGTGT GCATGTGCAA CAACGAGACG CCCAACCGTG TCATGCTCGA CTATTATCGT 
GAGGGCCAGG GCAAGGCTGG CCGCACCAGC CCAGAGGGCC GCGGGCGCCG GTCACCTGTC 
CTCTTGCCCA AGGGGCTGTT GGCCACAGAG GTGGGCCGAG CAGATGGTGG GACTGGGGAC 
AACAGTCCTT CACCCAGTTC TTCACTGCCG TCACCTCTGA GTGACCCCCG CCGGGAACCT 
ATGAACATCT ACAACCTGAT CGCCATCATC CGAGACCAGA TCAAGCACCT GCAGGCAGCC 
GTGGACCGTA CGACCGAGCT GTCCCGGCAG CGGATTGCCT CGCAGGAGCT GGGCCCTGCT 
GTGGACAAGG ACAAGGAAGC ACTCATGGAG GAGATCCTCA AGCTGAAGTC CCTGCTGAGT 
ACCAAGCGGG AGCAGATCAC CACACTGCGC ACCGTGCTCA AGGCCAACAA GCAGACAGCT 
GAGGTGGCCC TGGCCAACCT GAAGAGCAAG TATGAGAACG AGAAGGCCAT GGTGACCGAG 
ACCATGATGA AGCTACGGAA CGAGCTCAAG GCCCTCAAGG AAGACGCAGC CACCTTCTCC 
TCCCTGCGTG CCATGTTTGC CACCAGGTGT GACGAATACA TCACACAGCT GGATGAGATG 
CAGCGGCAGC TGGCAGCTGC CGAGGACGAG AAGAAGACCC TTAACTCTCT GTTGCGCATG 
GCCATCCAGC AGAAGCTGGC GCTCACCCAG CGGCTGGAGC TGCTGGAGCT GGACCATGAA 
CAGACCCGCA GGGGCCGCAG CAAGGCCGCC TCCAAGGCCA AGCCAGCCTC ACCGAGCGTA 
AGTCACACCT GCGCCTGCGC CAGCGAGAGG GCGGAGGGCG CCGGGCTGGC CAACCAGGTG 
TTCTGCAGCG AGAAGCACAG CATTTACTGT GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034268.1
CDS	123..2678
Translation	MSAPSEEEEYARLVMEAQPEWLRAEVKRLSHELAETTREKIQAAEYGLAVLEEKHQLKLQFEELEVDYEAIRSEMEQLKEAFGQAHTNHKKVAADGESREESLIQESASKEQYYVRKVLELQTELKQLRNVLTNTQSENERLTSVAQELKEINQNVEIQRGRLRDDIKEYKFREARLLQDYSELEEENISLQKQVSVLRQNQVEFEGLKHEIKRLEEETEYLNSQLEDAIRLKEISERQLEEALETLKTEREQKNNLRKELSHYMSINDSFYTSHLQVSLDGLKFSDDTVTAEPNNDAEALVNGFEHSGLVKSSLDNKTSTPRKDGLAPPSPSLVSDLLSELHISEIQKLKQQLVQMEREKVGLLATLQDTQKQLEQARGTLSEQHEKVNRLTENLSALRRLQAGKERQTSLDNEKDRDSHEDGDYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKISLLEKASHQDRELLAHLEKELKKVSDVAGETQGSLNVAQDELVTFSEELANLYHHVCMCNNETPNRVMLDYYREGQGKAGRTSPEGRGRRSPVLLPKGLLATEVGRADGGTGDNSPSPSSSLPSPLSDPRREPMNIYNLIAIIRDQIKHLQAAVDRTTELSRQRIASQELGPAVDKDKEALMEEILKLKSLLSTKREQITTLRTVLKANKQTAEVALANLKSKYENEKAMVTETMMKLRNELKALKEDAATFSSLRAMFATRCDEYITQLDEMQRQLAAAEDEKKTLNSLLRMAIQQKLALTQRLELLELDHEQTRRGRSKAASKAKPASPSVSHTCACASERAEGAGLANQVFCSEKHSIYCD

Target ORF information:

RefSeq Version	NM_001039179.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039179.2

ORF Insert Sequence:

ATGTCCGCGC CGTCGGAGGA GGAGGAGTAT GCGCGTCTGG TGATGGAGGC GCAGCCGGAG 
TGGCTGCGCG CCGAGGTGAA GCGGCTGTCC CACGAGCTGG CCGAGACCAC GCGTGAGAAG 
ATCCAGGCGG CCGAGTATGG ACTGGCGGTG CTGGAGGAGA AGCACCAGCT CAAGCTGCAG 
TTCGAGGAGC TCGAGGTGGA CTATGAGGCC ATCCGCAGCG AGATGGAGCA GCTCAAAGAG 
GCATTTGGCC AGGCACATAC AAACCACAAG AAGGTGGCTG CTGATGGTGA GAGCCGGGAG 
GAGAGCCTGA TCCAGGAGTC GGCCTCCAAG GAGCAGTACT ACGTGCGGAA GGTGCTGGAG 
CTGCAGACAG AGCTGAAACA GCTGCGCAAC GTCCTCACCA ACACCCAGTC TGAGAATGAG 
CGCCTCACGT CTGTGGCCCA GGAGCTAAAA GAGATCAACC AGAATGTGGA GATCCAGCGT 
GGTCGCCTGC GGGATGACAT CAAGGAGTAC AAGTTCCGGG AGGCCCGTCT ACTTCAGGAC 
TACTCAGAGC TGGAGGAGGA GAACATCAGC CTGCAGAAAC AAGTGTCTGT GCTCAGGCAG 
AACCAGGTGG AGTTTGAGGG CCTCAAGCAT GAAATCAAGC GCCTGGAGGA GGAGACAGAG 
TACCTCAACA GCCAGCTAGA GGATGCCATC CGGCTTAAGG AGATCTCTGA ACGGCAGTTG 
GAGGAGGCGC TGGAGACACT GAAGACAGAG CGGGAGCAGA AGAACAACCT GCGCAAGGAG 
TTGTCGCACT ACATGAGCAT CAACGATTCC TTCTATACCA GCCACCTGCA GGTCTCCTTA 
GATGGCCTCA AGTTCAGTGA TGATACTGTC ACCGCAGAGC CCAACAATGA CGCCGAAGCC 
CTGGTCAATG GCTTTGAGCA CAGTGGCTTG GTCAAATCAT CCTTGGACAA CAAGACATCC 
ACACCCAGGA AGGATGGCCT AGCTCCACCC TCCCCCAGCC TCGTCTCTGA CCTGCTCAGT 
GAGCTCCACA TATCTGAGAT CCAGAAGCTG AAACAGCAGC TGGTGCAGAT GGAGCGGGAG 
AAGGTGGGCC TGCTGGCAAC ACTGCAGGAC ACACAGAAGC AGCTGGAGCA GGCTCGGGGA 
ACCCTCTCTG AGCAGCATGA GAAGGTGAAT CGTCTCACAG AGAACCTTAG TGCCCTCCGG 
CGCCTGCAGG CTGGCAAGGA GCGACAGACC TCGTTGGATA ATGAGAAGGA CCGTGACAGC 
CACGAAGACG GTGACTACTA TGAGGTGGAC ATCAATGGGC CTGAGATCCT GGCCTGCAAG 
TACCACGTGG CTGTGGCTGA GGCTGGCGAG CTCCGGGAGC AGCTCAAGGC GTTGCGCAGC 
ACACACGAAG CTCGCGAAGC CCAGCACGCA GAAGAAAAGG GCCGGTATGA GGCTGAGGGC 
CAGGCACTCA CTGAGAAGAT CTCTCTGCTG GAGAAGGCTA GCCACCAGGA CCGAGAGCTG 
CTGGCCCATC TGGAAAAGGA GCTGAAGAAG GTGAGCGATG TGGCTGGTGA GACCCAGGGC 
AGCCTGAATG TGGCTCAGGA TGAGCTGGTG ACCTTCAGCG AGGAGCTGGC CAACCTCTAC 
CACCATGTGT GCATGTGCAA CAACGAGACG CCCAACCGTG TCATGCTCGA CTATTATCGT 
GAGGGCCAGG GCAAGGCTGG CCGCACCAGC CCAGAGGGCC GCGGGCGCCG GTCACCTGTC 
CTCTTGCCCA AGGGGCTGTT GGCCACAGAG GTGGGCCGAG CAGATGGTGG GACTGGGGAC 
AACAGTCCTT CACCCAGTTC TTCACTGCCG TCACCTCTGA GTGACCCCCG CCGGGAACCT 
ATGAACATCT ACAACCTGAT CGCCATCATC CGAGACCAGA TCAAGCACCT GCAGGCAGCC 
GTGGACCGTA CGACCGAGCT GTCCCGGCAG CGGATTGCCT CGCAGGAGCT GGGCCCTGCT 
GTGGACAAGG ACAAGGAAGC ACTCATGGAG GAGATCCTCA AGCTGAAGTC CCTGCTGAGT 
ACCAAGCGGG AGCAGATCAC CACACTGCGC ACCGTGCTCA AGGCCAACAA GCAGACAGCT 
GAGGTGGCCC TGGCCAACCT GAAGAGCAAG TATGAGAACG AGAAGGCCAT GGTGACCGAG 
ACCATGATGA AGCTACGGAA CGAGCTCAAG GCCCTCAAGG AAGACGCAGC CACCTTCTCC 
TCCCTGCGTG CCATGTTTGC CACCAGGTGT GACGAATACA TCACACAGCT GGATGAGATG 
CAGCGGCAGC TGGCAGCTGC CGAGGACGAG AAGAAGACCC TTAACTCTCT GTTGCGCATG 
GCCATCCAGC AGAAGCTGGC GCTCACCCAG CGGCTGGAGC TGCTGGAGCT GGACCATGAA 
CAGACCCGCA GGGGCCGCAG CAAGGCCGCC TCCAAGGCCA AGCCAGCCTC ACCGAGCGTA 
AGTCACACCT GCGCCTGCGC CAGCGAGAGG GCGGAGGGCG CCGGGCTGGC CAACCAGGTG 
TTCTGCAGCG AGAAGCACAG CATTTACTGT GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicd2 ( XM_011244429.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242731.1 Mus musculus bicaudal D homolog 2 (Drosophila) (Bicd2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu71587
Clone ID Related Accession (Same CDS sequence)	XM_011244429.2 , XM_011244429.3
Accession Version	XM_011244429.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein bicaudal D homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGATTTCGAG ACAGCAAGGG CCATGTCATG CCTGGAGGAC AGTGTTCCAC 
ATCACCAAGG TGGAGTTTGA GGGCCTCAAG CATGAAATCA AGCGCCTGGA GGAGGAGACA 
GAGTACCTCA ACAGCCAGCT AGAGGATGCC ATCCGGCTTA AGGAGATCTC TGAACGGCAG 
TTGGAGGAGG CGCTGGAGAC ACTGAAGACA GAGCGGGAGC AGAAGAACAA CCTGCGCAAG 
GAGTTGTCGC ACTACATGAG CATCAACGAT TCCTTCTATA CCAGCCACCT GCAGGTCTCC 
TTAGATGGCC TCAAGTTCAG TGATGATACT GTCACCGCAG AGCCCAACAA TGACGCCGAA 
GCCCTGGTCA ATGGCTTTGA GCACAGTGGC TTGGTCAAAT CATCCTTGGA CAACAAGACA 
TCCACACCCA GGAAGGATGG CCTAGCTCCA CCCTCCCCCA GCCTCGTCTC TGACCTGCTC 
AGTGAGCTCC ACATATCTGA GATCCAGAAG CTGAAACAGC AGCTGGTGCA GATGGAGCGG 
GAGAAGGTGG GCCTGCTGGC AACACTGCAG GACACACAGA AGCAGCTGGA GCAGGCTCGG 
GGAACCCTCT CTGAGCAGCA TGAGAAGGTG AATCGTCTCA CAGAGAACCT TAGTGCCCTC 
CGGCGCCTGC AGGCTGGCAA GGAGCGACAG ACCTCGTTGG ATAATGAGAA GGACCGTGAC 
AGCCACGAAG ACGGTGACTA CTATGAGGTG GACATCAATG GGCCTGAGAT CCTGGCCTGC 
AAGTACCACG TGGCTGTGGC TGAGGCTGGC GAGCTCCGGG AGCAGCTCAA GGCGTTGCGC 
AGCACACACG AAGCTCGCGA AGCCCAGCAC GCAGAAGAAA AGGGCCGGTA TGAGGCTGAG 
GGCCAGGCAC TCACTGAGAA GATCTCTCTG CTGGAGAAGG CTAGCCACCA GGACCGAGAG 
CTGCTGGCCC ATCTGGAAAA GGAGCTGAAG AAGGTGAGCG ATGTGGCTGG TGAGACCCAG 
GGCAGCCTGA ATGTGGCTCA GGATGAGCTG GTGACCTTCA GCGAGGAGCT GGCCAACCTC 
TACCACCATG TGTGCATGTG CAACAACGAG ACGCCCAACC GTGTCATGCT CGACTATTAT 
CGTGAGGGCC AGGGCAAGGC TGGCCGCACC AGCCCAGAGG GCCGCGGGCG CCGGTCACCT 
GTCCTCTTGC CCAAGGGGCT GTTGGCCACA GAGGTGGGCC GAGCAGATGG TGGGACTGGG 
GACAACAGTC CTTCACCCAG TTCTTCACTG CCGTCACCTC TGAGTGACCC CCGCCGGGAA 
CCTATGAACA TCTACAACCT GATCGCCATC ATCCGAGACC AGATCAAGCA CCTGCAGGCA 
GCCGTGGACC GTACGACCGA GCTGTCCCGG CAGCGGATTG CCTCGCAGGA GCTGGGCCCT 
GCTGTGGACA AGGACAAGGA AGCACTCATG GAGGAGATCC TCAAGCTGAA GTCCCTGCTG 
AGTACCAAGC GGGAGCAGAT CACCACACTG CGCACCGTGC TCAAGGCCAA CAAGCAGACA 
GCTGAGGTGG CCCTGGCCAA CCTGAAGAGC AAGTATGAGA ACGAGAAGGC CATGGTGACC 
GAGACCATGA TGAAGCTACG GAACGAGCTC AAGGCCCTCA AGGAAGACGC AGCCACCTTC 
TCCTCCCTGC GTGCCATGTT TGCCACCAGG TGTGACGAAT ACATCACACA GCTGGATGAG 
ATGCAGCGGC AGCTGGCAGC TGCCGAGGAC GAGAAGAAGA CCCTTAACTC TCTGTTGCGC 
ATGGCCATCC AGCAGAAGCT GGCGCTCACC CAGCGGCTGG AGCTGCTGGA GCTGGACCAT 
GAACAGACCC GCAGGGGCCG CAGCAAGGCC GCCTCCAAGG CCAAGCCAGC CTCACCGAGC 
GTAAGTCACA CCTGCGCCTG CGCCAGCGAG AGGGCGGAGG GCGCCGGGCT GGCCAACCAG 
GTGTTCTGCA GCGAGAAGCA CAGCATTTAC TGTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242731.1
CDS	306..2324
Translation	MAALISRQQGPCHAWRTVFHITKVEFEGLKHEIKRLEEETEYLNSQLEDAIRLKEISERQLEEALETLKTEREQKNNLRKELSHYMSINDSFYTSHLQVSLDGLKFSDDTVTAEPNNDAEALVNGFEHSGLVKSSLDNKTSTPRKDGLAPPSPSLVSDLLSELHISEIQKLKQQLVQMEREKVGLLATLQDTQKQLEQARGTLSEQHEKVNRLTENLSALRRLQAGKERQTSLDNEKDRDSHEDGDYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKISLLEKASHQDRELLAHLEKELKKVSDVAGETQGSLNVAQDELVTFSEELANLYHHVCMCNNETPNRVMLDYYREGQGKAGRTSPEGRGRRSPVLLPKGLLATEVGRADGGTGDNSPSPSSSLPSPLSDPRREPMNIYNLIAIIRDQIKHLQAAVDRTTELSRQRIASQELGPAVDKDKEALMEEILKLKSLLSTKREQITTLRTVLKANKQTAEVALANLKSKYENEKAMVTETMMKLRNELKALKEDAATFSSLRAMFATRCDEYITQLDEMQRQLAAAEDEKKTLNSLLRMAIQQKLALTQRLELLELDHEQTRRGRSKAASKAKPASPSVSHTCACASERAEGAGLANQVFCSEKHSIYCD

Target ORF information:

RefSeq Version	XM_011244429.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bicaudal D homolog 2 (Drosophila) (Bicd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244429.2

ORF Insert Sequence:

ATGGCTGCTC TGATTTCGAG ACAGCAAGGG CCATGTCATG CCTGGAGGAC AGTGTTCCAC 
ATCACCAAGG TGGAGTTTGA GGGCCTCAAG CATGAAATCA AGCGCCTGGA GGAGGAGACA 
GAGTACCTCA ACAGCCAGCT AGAGGATGCC ATCCGGCTTA AGGAGATCTC TGAACGGCAG 
TTGGAGGAGG CGCTGGAGAC ACTGAAGACA GAGCGGGAGC AGAAGAACAA CCTGCGCAAG 
GAGTTGTCGC ACTACATGAG CATCAACGAT TCCTTCTATA CCAGCCACCT GCAGGTCTCC 
TTAGATGGCC TCAAGTTCAG TGATGATACT GTCACCGCAG AGCCCAACAA TGACGCCGAA 
GCCCTGGTCA ATGGCTTTGA GCACAGTGGC TTGGTCAAAT CATCCTTGGA CAACAAGACA 
TCCACACCCA GGAAGGATGG CCTAGCTCCA CCCTCCCCCA GCCTCGTCTC TGACCTGCTC 
AGTGAGCTCC ACATATCTGA GATCCAGAAG CTGAAACAGC AGCTGGTGCA GATGGAGCGG 
GAGAAGGTGG GCCTGCTGGC AACACTGCAG GACACACAGA AGCAGCTGGA GCAGGCTCGG 
GGAACCCTCT CTGAGCAGCA TGAGAAGGTG AATCGTCTCA CAGAGAACCT TAGTGCCCTC 
CGGCGCCTGC AGGCTGGCAA GGAGCGACAG ACCTCGTTGG ATAATGAGAA GGACCGTGAC 
AGCCACGAAG ACGGTGACTA CTATGAGGTG GACATCAATG GGCCTGAGAT CCTGGCCTGC 
AAGTACCACG TGGCTGTGGC TGAGGCTGGC GAGCTCCGGG AGCAGCTCAA GGCGTTGCGC 
AGCACACACG AAGCTCGCGA AGCCCAGCAC GCAGAAGAAA AGGGCCGGTA TGAGGCTGAG 
GGCCAGGCAC TCACTGAGAA GATCTCTCTG CTGGAGAAGG CTAGCCACCA GGACCGAGAG 
CTGCTGGCCC ATCTGGAAAA GGAGCTGAAG AAGGTGAGCG ATGTGGCTGG TGAGACCCAG 
GGCAGCCTGA ATGTGGCTCA GGATGAGCTG GTGACCTTCA GCGAGGAGCT GGCCAACCTC 
TACCACCATG TGTGCATGTG CAACAACGAG ACGCCCAACC GTGTCATGCT CGACTATTAT 
CGTGAGGGCC AGGGCAAGGC TGGCCGCACC AGCCCAGAGG GCCGCGGGCG CCGGTCACCT 
GTCCTCTTGC CCAAGGGGCT GTTGGCCACA GAGGTGGGCC GAGCAGATGG TGGGACTGGG 
GACAACAGTC CTTCACCCAG TTCTTCACTG CCGTCACCTC TGAGTGACCC CCGCCGGGAA 
CCTATGAACA TCTACAACCT GATCGCCATC ATCCGAGACC AGATCAAGCA CCTGCAGGCA 
GCCGTGGACC GTACGACCGA GCTGTCCCGG CAGCGGATTG CCTCGCAGGA GCTGGGCCCT 
GCTGTGGACA AGGACAAGGA AGCACTCATG GAGGAGATCC TCAAGCTGAA GTCCCTGCTG 
AGTACCAAGC GGGAGCAGAT CACCACACTG CGCACCGTGC TCAAGGCCAA CAAGCAGACA 
GCTGAGGTGG CCCTGGCCAA CCTGAAGAGC AAGTATGAGA ACGAGAAGGC CATGGTGACC 
GAGACCATGA TGAAGCTACG GAACGAGCTC AAGGCCCTCA AGGAAGACGC AGCCACCTTC 
TCCTCCCTGC GTGCCATGTT TGCCACCAGG TGTGACGAAT ACATCACACA GCTGGATGAG 
ATGCAGCGGC AGCTGGCAGC TGCCGAGGAC GAGAAGAAGA CCCTTAACTC TCTGTTGCGC 
ATGGCCATCC AGCAGAAGCT GGCGCTCACC CAGCGGCTGG AGCTGCTGGA GCTGGACCAT 
GAACAGACCC GCAGGGGCCG CAGCAAGGCC GCCTCCAAGG CCAAGCCAGC CTCACCGAGC 
GTAAGTCACA CCTGCGCCTG CGCCAGCGAG AGGGCGGAGG GCGCCGGGCT GGCCAACCAG 
GTGTTCTGCA GCGAGAAGCA CAGCATTTAC TGTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicd2 ( NM_001039180.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034269.1 Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant 3, mRNA.

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CloneID	OMu00506
Clone ID Related Accession (Same CDS sequence)	NM_001039180.2
Accession Version	NM_001039180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	protein bicaudal D homolog 2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF538535.1, AK122348.1 and BP775855.1. On Oct 22, 2013 this sequence version replaced NM_001039180.1. Transcript Variant: This variant (3) differs in both UTRs and the 3' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK171300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGC TCAAAGAGGC ATTTGGCCAG GCACATACAA ACCACAAGAA GGTGGCTGCT 
GATGGTGAGA GCCGGGAGGA GAGCCTGATC CAGGAGTCGG CCTCCAAGGA GCAGTACTAC 
GTGCGGAAGG TGCTGGAGCT GCAGACAGAG CTGAAACAGC TGCGCAACGT CCTCACCAAC 
ACCCAGTCTG AGAATGAGCG CCTCACGTCT GTGGCCCAGG AGCTAAAAGA GATCAACCAG 
AATGTGGAGA TCCAGCGTGG TCGCCTGCGG GATGACATCA AGGAGTACAA GTTCCGGGAG 
GCCCGTCTAC TTCAGGACTA CTCAGAGCTG GAGGAGGAGA ACATCAGCCT GCAGAAACAA 
GTGTCTGTGC TCAGGCAGAA CCAGGTGGAG TTTGAGGGCC TCAAGCATGA AATCAAGCGC 
CTGGAGGAGG AGACAGAGTA CCTCAACAGC CAGCTAGAGG ATGCCATCCG GCTTAAGGAG 
ATCTCTGAAC GGCAGTTGGA GGAGGCGCTG GAGACACTGA AGACAGAGCG GGAGCAGAAG 
AACAACCTGC GCAAGGAGTT GTCGCACTAC ATGAGCATCA ACGATTCCTT CTATACCAGC 
CACCTGCAGG TCTCCTTAGA TGGCCTCAAG TTCAGTGATG ATACTGTCAC CGCAGAGCCC 
AACAATGACG CCGAAGCCCT GGTCAATGGC TTTGAGCACA GTGGCTTGGT CAAATCATCC 
TTGGACAACA AGACATCCAC ACCCAGGAAG GATGGCCTAG CTCCACCCTC CCCCAGCCTC 
GTCTCTGACC TGCTCAGTGA GCTCCACATA TCTGAGATCC AGAAGCTGAA ACAGCAGCTG 
GTGCAGATGG AGCGGGAGAA GGTGGGCCTG CTGGCAACAC TGCAGGACAC ACAGAAGCAG 
CTGGAGCAGG CTCGGGGAAC CCTCTCTGAG CAGCATGAGA AGGTGAATCG TCTCACAGAG 
AACCTTAGTG CCCTCCGGCG CCTGCAGGCT GGCAAGGAGC GACAGACCTC GTTGGATAAT 
GAGAAGGACC GTGACAGCCA CGAAGACGGT GACTACTATG AGGTGGACAT CAATGGGCCT 
GAGATCCTGG CCTGCAAGTA CCACGTGGCT GTGGCTGAGG CTGGCGAGCT CCGGGAGCAG 
CTCAAGGCGT TGCGCAGCAC ACACGAAGCT CGCGAAGCCC AGCACGCAGA AGAAAAGGGC 
CGGTATGAGG CTGAGGGCCA GGCACTCACT GAGAAGATCT CTCTGCTGGA GAAGGCTAGC 
CACCAGGACC GAGAGCTGCT GGCCCATCTG GAAAAGGAGC TGAAGAAGGT GAGCGATGTG 
GCTGGTGAGA CCCAGGGCAG CCTGAATGTG GCTCAGGATG AGCTGGTGAC CTTCAGCGAG 
GAGCTGGCCA ACCTCTACCA CCATGTGTGC ATGTGCAACA ACGAGACGCC CAACCGTGTC 
ATGCTCGACT ATTATCGTGA GGGCCAGGGC AAGGCTGGCC GCACCAGCCC AGAGGGCCGC 
GGGCGCCGGT CACCTGTCCT CTTGCCCAAG GGGCTGTTGG CCACAGAGGT GGGCCGAGCA 
GATGGTGGGA CTGGGGACAA CAGTCCTTCA CCCAGTTCTT CACTGCCGTC ACCTCTGAGT 
GACCCCCGCC GGGAACCTAT GAACATCTAC AACCTGATCG CCATCATCCG AGACCAGATC 
AAGCACCTGC AGGCAGCCGT GGACCGTACG ACCGAGCTGT CCCGGCAGCG GATTGCCTCG 
CAGGAGCTGG GCCCTGCTGT GGACAAGGAC AAGGAAGCAC TCATGGAGGA GATCCTCAAG 
CTGAAGTCCC TGCTGAGTAC CAAGCGGGAG CAGATCACCA CACTGCGCAC CGTGCTCAAG 
GCCAACAAGC AGACAGCTGA GGTGGCCCTG GCCAACCTGA AGAGCAAGTA TGAGAACGAG 
AAGGCCATGG TGACCGAGAC CATGATGAAG CTACGGAACG AGCTCAAGGC CCTCAAGGAA 
GACGCAGCCA CCTTCTCCTC CCTGCGTGCC ATGTTTGCCA CCAGGTGTGA CGAATACATC 
ACACAGCTGG ATGAGATGCA GCGGCAGCTG GCAGCTGCCG AGGACGAGAA GAAGACCCTT 
AACTCTCTGT TGCGCATGGC CATCCAGCAG AAGCTGGCGC TCACCCAGCG GCTGGAGCTG 
CTGGAGCTGG ACCATGAACA GACCCGCAGG GGCCGCAGCA AGGCCGCCTC CAAGGCCAAG 
CCAGCCTCAC CGAGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034269.1
CDS	241..2481
Translation	MEQLKEAFGQAHTNHKKVAADGESREESLIQESASKEQYYVRKVLELQTELKQLRNVLTNTQSENERLTSVAQELKEINQNVEIQRGRLRDDIKEYKFREARLLQDYSELEEENISLQKQVSVLRQNQVEFEGLKHEIKRLEEETEYLNSQLEDAIRLKEISERQLEEALETLKTEREQKNNLRKELSHYMSINDSFYTSHLQVSLDGLKFSDDTVTAEPNNDAEALVNGFEHSGLVKSSLDNKTSTPRKDGLAPPSPSLVSDLLSELHISEIQKLKQQLVQMEREKVGLLATLQDTQKQLEQARGTLSEQHEKVNRLTENLSALRRLQAGKERQTSLDNEKDRDSHEDGDYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKISLLEKASHQDRELLAHLEKELKKVSDVAGETQGSLNVAQDELVTFSEELANLYHHVCMCNNETPNRVMLDYYREGQGKAGRTSPEGRGRRSPVLLPKGLLATEVGRADGGTGDNSPSPSSSLPSPLSDPRREPMNIYNLIAIIRDQIKHLQAAVDRTTELSRQRIASQELGPAVDKDKEALMEEILKLKSLLSTKREQITTLRTVLKANKQTAEVALANLKSKYENEKAMVTETMMKLRNELKALKEDAATFSSLRAMFATRCDEYITQLDEMQRQLAAAEDEKKTLNSLLRMAIQQKLALTQRLELLELDHEQTRRGRSKAASKAKPASPSL

Target ORF information:

RefSeq Version	NM_001039180.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039180.2

ORF Insert Sequence:

ATGGAGCAGC TCAAAGAGGC ATTTGGCCAG GCACATACAA ACCACAAGAA GGTGGCTGCT 
GATGGTGAGA GCCGGGAGGA GAGCCTGATC CAGGAGTCGG CCTCCAAGGA GCAGTACTAC 
GTGCGGAAGG TGCTGGAGCT GCAGACAGAG CTGAAACAGC TGCGCAACGT CCTCACCAAC 
ACCCAGTCTG AGAATGAGCG CCTCACGTCT GTGGCCCAGG AGCTAAAAGA GATCAACCAG 
AATGTGGAGA TCCAGCGTGG TCGCCTGCGG GATGACATCA AGGAGTACAA GTTCCGGGAG 
GCCCGTCTAC TTCAGGACTA CTCAGAGCTG GAGGAGGAGA ACATCAGCCT GCAGAAACAA 
GTGTCTGTGC TCAGGCAGAA CCAGGTGGAG TTTGAGGGCC TCAAGCATGA AATCAAGCGC 
CTGGAGGAGG AGACAGAGTA CCTCAACAGC CAGCTAGAGG ATGCCATCCG GCTTAAGGAG 
ATCTCTGAAC GGCAGTTGGA GGAGGCGCTG GAGACACTGA AGACAGAGCG GGAGCAGAAG 
AACAACCTGC GCAAGGAGTT GTCGCACTAC ATGAGCATCA ACGATTCCTT CTATACCAGC 
CACCTGCAGG TCTCCTTAGA TGGCCTCAAG TTCAGTGATG ATACTGTCAC CGCAGAGCCC 
AACAATGACG CCGAAGCCCT GGTCAATGGC TTTGAGCACA GTGGCTTGGT CAAATCATCC 
TTGGACAACA AGACATCCAC ACCCAGGAAG GATGGCCTAG CTCCACCCTC CCCCAGCCTC 
GTCTCTGACC TGCTCAGTGA GCTCCACATA TCTGAGATCC AGAAGCTGAA ACAGCAGCTG 
GTGCAGATGG AGCGGGAGAA GGTGGGCCTG CTGGCAACAC TGCAGGACAC ACAGAAGCAG 
CTGGAGCAGG CTCGGGGAAC CCTCTCTGAG CAGCATGAGA AGGTGAATCG TCTCACAGAG 
AACCTTAGTG CCCTCCGGCG CCTGCAGGCT GGCAAGGAGC GACAGACCTC GTTGGATAAT 
GAGAAGGACC GTGACAGCCA CGAAGACGGT GACTACTATG AGGTGGACAT CAATGGGCCT 
GAGATCCTGG CCTGCAAGTA CCACGTGGCT GTGGCTGAGG CTGGCGAGCT CCGGGAGCAG 
CTCAAGGCGT TGCGCAGCAC ACACGAAGCT CGCGAAGCCC AGCACGCAGA AGAAAAGGGC 
CGGTATGAGG CTGAGGGCCA GGCACTCACT GAGAAGATCT CTCTGCTGGA GAAGGCTAGC 
CACCAGGACC GAGAGCTGCT GGCCCATCTG GAAAAGGAGC TGAAGAAGGT GAGCGATGTG 
GCTGGTGAGA CCCAGGGCAG CCTGAATGTG GCTCAGGATG AGCTGGTGAC CTTCAGCGAG 
GAGCTGGCCA ACCTCTACCA CCATGTGTGC ATGTGCAACA ACGAGACGCC CAACCGTGTC 
ATGCTCGACT ATTATCGTGA GGGCCAGGGC AAGGCTGGCC GCACCAGCCC AGAGGGCCGC 
GGGCGCCGGT CACCTGTCCT CTTGCCCAAG GGGCTGTTGG CCACAGAGGT GGGCCGAGCA 
GATGGTGGGA CTGGGGACAA CAGTCCTTCA CCCAGTTCTT CACTGCCGTC ACCTCTGAGT 
GACCCCCGCC GGGAACCTAT GAACATCTAC AACCTGATCG CCATCATCCG AGACCAGATC 
AAGCACCTGC AGGCAGCCGT GGACCGTACG ACCGAGCTGT CCCGGCAGCG GATTGCCTCG 
CAGGAGCTGG GCCCTGCTGT GGACAAGGAC AAGGAAGCAC TCATGGAGGA GATCCTCAAG 
CTGAAGTCCC TGCTGAGTAC CAAGCGGGAG CAGATCACCA CACTGCGCAC CGTGCTCAAG 
GCCAACAAGC AGACAGCTGA GGTGGCCCTG GCCAACCTGA AGAGCAAGTA TGAGAACGAG 
AAGGCCATGG TGACCGAGAC CATGATGAAG CTACGGAACG AGCTCAAGGC CCTCAAGGAA 
GACGCAGCCA CCTTCTCCTC CCTGCGTGCC ATGTTTGCCA CCAGGTGTGA CGAATACATC 
ACACAGCTGG ATGAGATGCA GCGGCAGCTG GCAGCTGCCG AGGACGAGAA GAAGACCCTT 
AACTCTCTGT TGCGCATGGC CATCCAGCAG AAGCTGGCGC TCACCCAGCG GCTGGAGCTG 
CTGGAGCTGG ACCATGAACA GACCCGCAGG GGCCGCAGCA AGGCCGCCTC CAAGGCCAAG 
CCAGCCTCAC CGAGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicd2 ( NM_029791.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084067.1 Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant 2, mRNA.

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CloneID	OMu07477
Clone ID Related Accession (Same CDS sequence)	NM_029791.4
Accession Version	NM_029791.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein bicaudal D homolog 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF538535.1, AK122348.1 and BP775855.1. On Oct 22, 2013 this sequence version replaced NM_029791.3. Transcript Variant: This variant (2) differs in the 3' coding sequence and 3' UTR, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032198.1, AJ250106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCGC CGTCGGAGGA GGAGGAGTAT GCGCGTCTGG TGATGGAGGC GCAGCCGGAG 
TGGCTGCGCG CCGAGGTGAA GCGGCTGTCC CACGAGCTGG CCGAGACCAC GCGTGAGAAG 
ATCCAGGCGG CCGAGTATGG ACTGGCGGTG CTGGAGGAGA AGCACCAGCT CAAGCTGCAG 
TTCGAGGAGC TCGAGGTGGA CTATGAGGCC ATCCGCAGCG AGATGGAGCA GCTCAAAGAG 
GCATTTGGCC AGGCACATAC AAACCACAAG AAGGTGGCTG CTGATGGTGA GAGCCGGGAG 
GAGAGCCTGA TCCAGGAGTC GGCCTCCAAG GAGCAGTACT ACGTGCGGAA GGTGCTGGAG 
CTGCAGACAG AGCTGAAACA GCTGCGCAAC GTCCTCACCA ACACCCAGTC TGAGAATGAG 
CGCCTCACGT CTGTGGCCCA GGAGCTAAAA GAGATCAACC AGAATGTGGA GATCCAGCGT 
GGTCGCCTGC GGGATGACAT CAAGGAGTAC AAGTTCCGGG AGGCCCGTCT ACTTCAGGAC 
TACTCAGAGC TGGAGGAGGA GAACATCAGC CTGCAGAAAC AAGTGTCTGT GCTCAGGCAG 
AACCAGGTGG AGTTTGAGGG CCTCAAGCAT GAAATCAAGC GCCTGGAGGA GGAGACAGAG 
TACCTCAACA GCCAGCTAGA GGATGCCATC CGGCTTAAGG AGATCTCTGA ACGGCAGTTG 
GAGGAGGCGC TGGAGACACT GAAGACAGAG CGGGAGCAGA AGAACAACCT GCGCAAGGAG 
TTGTCGCACT ACATGAGCAT CAACGATTCC TTCTATACCA GCCACCTGCA GGTCTCCTTA 
GATGGCCTCA AGTTCAGTGA TGATACTGTC ACCGCAGAGC CCAACAATGA CGCCGAAGCC 
CTGGTCAATG GCTTTGAGCA CAGTGGCTTG GTCAAATCAT CCTTGGACAA CAAGACATCC 
ACACCCAGGA AGGATGGCCT AGCTCCACCC TCCCCCAGCC TCGTCTCTGA CCTGCTCAGT 
GAGCTCCACA TATCTGAGAT CCAGAAGCTG AAACAGCAGC TGGTGCAGAT GGAGCGGGAG 
AAGGTGGGCC TGCTGGCAAC ACTGCAGGAC ACACAGAAGC AGCTGGAGCA GGCTCGGGGA 
ACCCTCTCTG AGCAGCATGA GAAGGTGAAT CGTCTCACAG AGAACCTTAG TGCCCTCCGG 
CGCCTGCAGG CTGGCAAGGA GCGACAGACC TCGTTGGATA ATGAGAAGGA CCGTGACAGC 
CACGAAGACG GTGACTACTA TGAGGTGGAC ATCAATGGGC CTGAGATCCT GGCCTGCAAG 
TACCACGTGG CTGTGGCTGA GGCTGGCGAG CTCCGGGAGC AGCTCAAGGC GTTGCGCAGC 
ACACACGAAG CTCGCGAAGC CCAGCACGCA GAAGAAAAGG GCCGGTATGA GGCTGAGGGC 
CAGGCACTCA CTGAGAAGAT CTCTCTGCTG GAGAAGGCTA GCCACCAGGA CCGAGAGCTG 
CTGGCCCATC TGGAAAAGGA GCTGAAGAAG GTGAGCGATG TGGCTGGTGA GACCCAGGGC 
AGCCTGAATG TGGCTCAGGA TGAGCTGGTG ACCTTCAGCG AGGAGCTGGC CAACCTCTAC 
CACCATGTGT GCATGTGCAA CAACGAGACG CCCAACCGTG TCATGCTCGA CTATTATCGT 
GAGGGCCAGG GCAAGGCTGG CCGCACCAGC CCAGAGGGCC GCGGGCGCCG GTCACCTGTC 
CTCTTGCCCA AGGGGCTGTT GGCCACAGAG GTGGGCCGAG CAGATGGTGG GACTGGGGAC 
AACAGTCCTT CACCCAGTTC TTCACTGCCG TCACCTCTGA GTGACCCCCG CCGGGAACCT 
ATGAACATCT ACAACCTGAT CGCCATCATC CGAGACCAGA TCAAGCACCT GCAGGCAGCC 
GTGGACCGTA CGACCGAGCT GTCCCGGCAG CGGATTGCCT CGCAGGAGCT GGGCCCTGCT 
GTGGACAAGG ACAAGGAAGC ACTCATGGAG GAGATCCTCA AGCTGAAGTC CCTGCTGAGT 
ACCAAGCGGG AGCAGATCAC CACACTGCGC ACCGTGCTCA AGGCCAACAA GCAGACAGCT 
GAGGTGGCCC TGGCCAACCT GAAGAGCAAG TATGAGAACG AGAAGGCCAT GGTGACCGAG 
ACCATGATGA AGCTACGGAA CGAGCTCAAG GCCCTCAAGG AAGACGCAGC CACCTTCTCC 
TCCCTGCGTG CCATGTTTGC CACCAGGTGT GACGAATACA TCACACAGCT GGATGAGATG 
CAGCGGCAGC TGGCAGCTGC CGAGGACGAG AAGAAGACCC TTAACTCTCT GTTGCGCATG 
GCCATCCAGC AGAAGCTGGC GCTCACCCAG CGGCTGGAGC TGCTGGAGCT GGACCATGAA 
CAGACCCGCA GGGGCCGCAG CAAGGCCGCC TCCAAGGCCA AGCCAGCCTC ACCGAGCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084067.1
CDS	123..2585
Translation	MSAPSEEEEYARLVMEAQPEWLRAEVKRLSHELAETTREKIQAAEYGLAVLEEKHQLKLQFEELEVDYEAIRSEMEQLKEAFGQAHTNHKKVAADGESREESLIQESASKEQYYVRKVLELQTELKQLRNVLTNTQSENERLTSVAQELKEINQNVEIQRGRLRDDIKEYKFREARLLQDYSELEEENISLQKQVSVLRQNQVEFEGLKHEIKRLEEETEYLNSQLEDAIRLKEISERQLEEALETLKTEREQKNNLRKELSHYMSINDSFYTSHLQVSLDGLKFSDDTVTAEPNNDAEALVNGFEHSGLVKSSLDNKTSTPRKDGLAPPSPSLVSDLLSELHISEIQKLKQQLVQMEREKVGLLATLQDTQKQLEQARGTLSEQHEKVNRLTENLSALRRLQAGKERQTSLDNEKDRDSHEDGDYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKISLLEKASHQDRELLAHLEKELKKVSDVAGETQGSLNVAQDELVTFSEELANLYHHVCMCNNETPNRVMLDYYREGQGKAGRTSPEGRGRRSPVLLPKGLLATEVGRADGGTGDNSPSPSSSLPSPLSDPRREPMNIYNLIAIIRDQIKHLQAAVDRTTELSRQRIASQELGPAVDKDKEALMEEILKLKSLLSTKREQITTLRTVLKANKQTAEVALANLKSKYENEKAMVTETMMKLRNELKALKEDAATFSSLRAMFATRCDEYITQLDEMQRQLAAAEDEKKTLNSLLRMAIQQKLALTQRLELLELDHEQTRRGRSKAASKAKPASPSL

Target ORF information:

RefSeq Version	NM_029791.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029791.4

ORF Insert Sequence:

ATGTCCGCGC CGTCGGAGGA GGAGGAGTAT GCGCGTCTGG TGATGGAGGC GCAGCCGGAG 
TGGCTGCGCG CCGAGGTGAA GCGGCTGTCC CACGAGCTGG CCGAGACCAC GCGTGAGAAG 
ATCCAGGCGG CCGAGTATGG ACTGGCGGTG CTGGAGGAGA AGCACCAGCT CAAGCTGCAG 
TTCGAGGAGC TCGAGGTGGA CTATGAGGCC ATCCGCAGCG AGATGGAGCA GCTCAAAGAG 
GCATTTGGCC AGGCACATAC AAACCACAAG AAGGTGGCTG CTGATGGTGA GAGCCGGGAG 
GAGAGCCTGA TCCAGGAGTC GGCCTCCAAG GAGCAGTACT ACGTGCGGAA GGTGCTGGAG 
CTGCAGACAG AGCTGAAACA GCTGCGCAAC GTCCTCACCA ACACCCAGTC TGAGAATGAG 
CGCCTCACGT CTGTGGCCCA GGAGCTAAAA GAGATCAACC AGAATGTGGA GATCCAGCGT 
GGTCGCCTGC GGGATGACAT CAAGGAGTAC AAGTTCCGGG AGGCCCGTCT ACTTCAGGAC 
TACTCAGAGC TGGAGGAGGA GAACATCAGC CTGCAGAAAC AAGTGTCTGT GCTCAGGCAG 
AACCAGGTGG AGTTTGAGGG CCTCAAGCAT GAAATCAAGC GCCTGGAGGA GGAGACAGAG 
TACCTCAACA GCCAGCTAGA GGATGCCATC CGGCTTAAGG AGATCTCTGA ACGGCAGTTG 
GAGGAGGCGC TGGAGACACT GAAGACAGAG CGGGAGCAGA AGAACAACCT GCGCAAGGAG 
TTGTCGCACT ACATGAGCAT CAACGATTCC TTCTATACCA GCCACCTGCA GGTCTCCTTA 
GATGGCCTCA AGTTCAGTGA TGATACTGTC ACCGCAGAGC CCAACAATGA CGCCGAAGCC 
CTGGTCAATG GCTTTGAGCA CAGTGGCTTG GTCAAATCAT CCTTGGACAA CAAGACATCC 
ACACCCAGGA AGGATGGCCT AGCTCCACCC TCCCCCAGCC TCGTCTCTGA CCTGCTCAGT 
GAGCTCCACA TATCTGAGAT CCAGAAGCTG AAACAGCAGC TGGTGCAGAT GGAGCGGGAG 
AAGGTGGGCC TGCTGGCAAC ACTGCAGGAC ACACAGAAGC AGCTGGAGCA GGCTCGGGGA 
ACCCTCTCTG AGCAGCATGA GAAGGTGAAT CGTCTCACAG AGAACCTTAG TGCCCTCCGG 
CGCCTGCAGG CTGGCAAGGA GCGACAGACC TCGTTGGATA ATGAGAAGGA CCGTGACAGC 
CACGAAGACG GTGACTACTA TGAGGTGGAC ATCAATGGGC CTGAGATCCT GGCCTGCAAG 
TACCACGTGG CTGTGGCTGA GGCTGGCGAG CTCCGGGAGC AGCTCAAGGC GTTGCGCAGC 
ACACACGAAG CTCGCGAAGC CCAGCACGCA GAAGAAAAGG GCCGGTATGA GGCTGAGGGC 
CAGGCACTCA CTGAGAAGAT CTCTCTGCTG GAGAAGGCTA GCCACCAGGA CCGAGAGCTG 
CTGGCCCATC TGGAAAAGGA GCTGAAGAAG GTGAGCGATG TGGCTGGTGA GACCCAGGGC 
AGCCTGAATG TGGCTCAGGA TGAGCTGGTG ACCTTCAGCG AGGAGCTGGC CAACCTCTAC 
CACCATGTGT GCATGTGCAA CAACGAGACG CCCAACCGTG TCATGCTCGA CTATTATCGT 
GAGGGCCAGG GCAAGGCTGG CCGCACCAGC CCAGAGGGCC GCGGGCGCCG GTCACCTGTC 
CTCTTGCCCA AGGGGCTGTT GGCCACAGAG GTGGGCCGAG CAGATGGTGG GACTGGGGAC 
AACAGTCCTT CACCCAGTTC TTCACTGCCG TCACCTCTGA GTGACCCCCG CCGGGAACCT 
ATGAACATCT ACAACCTGAT CGCCATCATC CGAGACCAGA TCAAGCACCT GCAGGCAGCC 
GTGGACCGTA CGACCGAGCT GTCCCGGCAG CGGATTGCCT CGCAGGAGCT GGGCCCTGCT 
GTGGACAAGG ACAAGGAAGC ACTCATGGAG GAGATCCTCA AGCTGAAGTC CCTGCTGAGT 
ACCAAGCGGG AGCAGATCAC CACACTGCGC ACCGTGCTCA AGGCCAACAA GCAGACAGCT 
GAGGTGGCCC TGGCCAACCT GAAGAGCAAG TATGAGAACG AGAAGGCCAT GGTGACCGAG 
ACCATGATGA AGCTACGGAA CGAGCTCAAG GCCCTCAAGG AAGACGCAGC CACCTTCTCC 
TCCCTGCGTG CCATGTTTGC CACCAGGTGT GACGAATACA TCACACAGCT GGATGAGATG 
CAGCGGCAGC TGGCAGCTGC CGAGGACGAG AAGAAGACCC TTAACTCTCT GTTGCGCATG 
GCCATCCAGC AGAAGCTGGC GCTCACCCAG CGGCTGGAGC TGCTGGAGCT GGACCATGAA 
CAGACCCGCA GGGGCCGCAG CAAGGCCGCC TCCAAGGCCA AGCCAGCCTC ACCGAGCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicd2 ( XM_011244429.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242731.1 Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant X1, mRNA.

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CloneID	OMu71587
Clone ID Related Accession (Same CDS sequence)	XM_011244429.2 , XM_011244429.3
Accession Version	XM_011244429.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein bicaudal D homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGATTTCGAG ACAGCAAGGG CCATGTCATG CCTGGAGGAC AGTGTTCCAC 
ATCACCAAGG TGGAGTTTGA GGGCCTCAAG CATGAAATCA AGCGCCTGGA GGAGGAGACA 
GAGTACCTCA ACAGCCAGCT AGAGGATGCC ATCCGGCTTA AGGAGATCTC TGAACGGCAG 
TTGGAGGAGG CGCTGGAGAC ACTGAAGACA GAGCGGGAGC AGAAGAACAA CCTGCGCAAG 
GAGTTGTCGC ACTACATGAG CATCAACGAT TCCTTCTATA CCAGCCACCT GCAGGTCTCC 
TTAGATGGCC TCAAGTTCAG TGATGATACT GTCACCGCAG AGCCCAACAA TGACGCCGAA 
GCCCTGGTCA ATGGCTTTGA GCACAGTGGC TTGGTCAAAT CATCCTTGGA CAACAAGACA 
TCCACACCCA GGAAGGATGG CCTAGCTCCA CCCTCCCCCA GCCTCGTCTC TGACCTGCTC 
AGTGAGCTCC ACATATCTGA GATCCAGAAG CTGAAACAGC AGCTGGTGCA GATGGAGCGG 
GAGAAGGTGG GCCTGCTGGC AACACTGCAG GACACACAGA AGCAGCTGGA GCAGGCTCGG 
GGAACCCTCT CTGAGCAGCA TGAGAAGGTG AATCGTCTCA CAGAGAACCT TAGTGCCCTC 
CGGCGCCTGC AGGCTGGCAA GGAGCGACAG ACCTCGTTGG ATAATGAGAA GGACCGTGAC 
AGCCACGAAG ACGGTGACTA CTATGAGGTG GACATCAATG GGCCTGAGAT CCTGGCCTGC 
AAGTACCACG TGGCTGTGGC TGAGGCTGGC GAGCTCCGGG AGCAGCTCAA GGCGTTGCGC 
AGCACACACG AAGCTCGCGA AGCCCAGCAC GCAGAAGAAA AGGGCCGGTA TGAGGCTGAG 
GGCCAGGCAC TCACTGAGAA GATCTCTCTG CTGGAGAAGG CTAGCCACCA GGACCGAGAG 
CTGCTGGCCC ATCTGGAAAA GGAGCTGAAG AAGGTGAGCG ATGTGGCTGG TGAGACCCAG 
GGCAGCCTGA ATGTGGCTCA GGATGAGCTG GTGACCTTCA GCGAGGAGCT GGCCAACCTC 
TACCACCATG TGTGCATGTG CAACAACGAG ACGCCCAACC GTGTCATGCT CGACTATTAT 
CGTGAGGGCC AGGGCAAGGC TGGCCGCACC AGCCCAGAGG GCCGCGGGCG CCGGTCACCT 
GTCCTCTTGC CCAAGGGGCT GTTGGCCACA GAGGTGGGCC GAGCAGATGG TGGGACTGGG 
GACAACAGTC CTTCACCCAG TTCTTCACTG CCGTCACCTC TGAGTGACCC CCGCCGGGAA 
CCTATGAACA TCTACAACCT GATCGCCATC ATCCGAGACC AGATCAAGCA CCTGCAGGCA 
GCCGTGGACC GTACGACCGA GCTGTCCCGG CAGCGGATTG CCTCGCAGGA GCTGGGCCCT 
GCTGTGGACA AGGACAAGGA AGCACTCATG GAGGAGATCC TCAAGCTGAA GTCCCTGCTG 
AGTACCAAGC GGGAGCAGAT CACCACACTG CGCACCGTGC TCAAGGCCAA CAAGCAGACA 
GCTGAGGTGG CCCTGGCCAA CCTGAAGAGC AAGTATGAGA ACGAGAAGGC CATGGTGACC 
GAGACCATGA TGAAGCTACG GAACGAGCTC AAGGCCCTCA AGGAAGACGC AGCCACCTTC 
TCCTCCCTGC GTGCCATGTT TGCCACCAGG TGTGACGAAT ACATCACACA GCTGGATGAG 
ATGCAGCGGC AGCTGGCAGC TGCCGAGGAC GAGAAGAAGA CCCTTAACTC TCTGTTGCGC 
ATGGCCATCC AGCAGAAGCT GGCGCTCACC CAGCGGCTGG AGCTGCTGGA GCTGGACCAT 
GAACAGACCC GCAGGGGCCG CAGCAAGGCC GCCTCCAAGG CCAAGCCAGC CTCACCGAGC 
GTAAGTCACA CCTGCGCCTG CGCCAGCGAG AGGGCGGAGG GCGCCGGGCT GGCCAACCAG 
GTGTTCTGCA GCGAGAAGCA CAGCATTTAC TGTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242731.1
CDS	305..2323
Translation	MAALISRQQGPCHAWRTVFHITKVEFEGLKHEIKRLEEETEYLNSQLEDAIRLKEISERQLEEALETLKTEREQKNNLRKELSHYMSINDSFYTSHLQVSLDGLKFSDDTVTAEPNNDAEALVNGFEHSGLVKSSLDNKTSTPRKDGLAPPSPSLVSDLLSELHISEIQKLKQQLVQMEREKVGLLATLQDTQKQLEQARGTLSEQHEKVNRLTENLSALRRLQAGKERQTSLDNEKDRDSHEDGDYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKISLLEKASHQDRELLAHLEKELKKVSDVAGETQGSLNVAQDELVTFSEELANLYHHVCMCNNETPNRVMLDYYREGQGKAGRTSPEGRGRRSPVLLPKGLLATEVGRADGGTGDNSPSPSSSLPSPLSDPRREPMNIYNLIAIIRDQIKHLQAAVDRTTELSRQRIASQELGPAVDKDKEALMEEILKLKSLLSTKREQITTLRTVLKANKQTAEVALANLKSKYENEKAMVTETMMKLRNELKALKEDAATFSSLRAMFATRCDEYITQLDEMQRQLAAAEDEKKTLNSLLRMAIQQKLALTQRLELLELDHEQTRRGRSKAASKAKPASPSVSHTCACASERAEGAGLANQVFCSEKHSIYCD

Target ORF information:

RefSeq Version	XM_011244429.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BICD cargo adaptor 2 (Bicd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244429.3

ORF Insert Sequence:

ATGGCTGCTC TGATTTCGAG ACAGCAAGGG CCATGTCATG CCTGGAGGAC AGTGTTCCAC 
ATCACCAAGG TGGAGTTTGA GGGCCTCAAG CATGAAATCA AGCGCCTGGA GGAGGAGACA 
GAGTACCTCA ACAGCCAGCT AGAGGATGCC ATCCGGCTTA AGGAGATCTC TGAACGGCAG 
TTGGAGGAGG CGCTGGAGAC ACTGAAGACA GAGCGGGAGC AGAAGAACAA CCTGCGCAAG 
GAGTTGTCGC ACTACATGAG CATCAACGAT TCCTTCTATA CCAGCCACCT GCAGGTCTCC 
TTAGATGGCC TCAAGTTCAG TGATGATACT GTCACCGCAG AGCCCAACAA TGACGCCGAA 
GCCCTGGTCA ATGGCTTTGA GCACAGTGGC TTGGTCAAAT CATCCTTGGA CAACAAGACA 
TCCACACCCA GGAAGGATGG CCTAGCTCCA CCCTCCCCCA GCCTCGTCTC TGACCTGCTC 
AGTGAGCTCC ACATATCTGA GATCCAGAAG CTGAAACAGC AGCTGGTGCA GATGGAGCGG 
GAGAAGGTGG GCCTGCTGGC AACACTGCAG GACACACAGA AGCAGCTGGA GCAGGCTCGG 
GGAACCCTCT CTGAGCAGCA TGAGAAGGTG AATCGTCTCA CAGAGAACCT TAGTGCCCTC 
CGGCGCCTGC AGGCTGGCAA GGAGCGACAG ACCTCGTTGG ATAATGAGAA GGACCGTGAC 
AGCCACGAAG ACGGTGACTA CTATGAGGTG GACATCAATG GGCCTGAGAT CCTGGCCTGC 
AAGTACCACG TGGCTGTGGC TGAGGCTGGC GAGCTCCGGG AGCAGCTCAA GGCGTTGCGC 
AGCACACACG AAGCTCGCGA AGCCCAGCAC GCAGAAGAAA AGGGCCGGTA TGAGGCTGAG 
GGCCAGGCAC TCACTGAGAA GATCTCTCTG CTGGAGAAGG CTAGCCACCA GGACCGAGAG 
CTGCTGGCCC ATCTGGAAAA GGAGCTGAAG AAGGTGAGCG ATGTGGCTGG TGAGACCCAG 
GGCAGCCTGA ATGTGGCTCA GGATGAGCTG GTGACCTTCA GCGAGGAGCT GGCCAACCTC 
TACCACCATG TGTGCATGTG CAACAACGAG ACGCCCAACC GTGTCATGCT CGACTATTAT 
CGTGAGGGCC AGGGCAAGGC TGGCCGCACC AGCCCAGAGG GCCGCGGGCG CCGGTCACCT 
GTCCTCTTGC CCAAGGGGCT GTTGGCCACA GAGGTGGGCC GAGCAGATGG TGGGACTGGG 
GACAACAGTC CTTCACCCAG TTCTTCACTG CCGTCACCTC TGAGTGACCC CCGCCGGGAA 
CCTATGAACA TCTACAACCT GATCGCCATC ATCCGAGACC AGATCAAGCA CCTGCAGGCA 
GCCGTGGACC GTACGACCGA GCTGTCCCGG CAGCGGATTG CCTCGCAGGA GCTGGGCCCT 
GCTGTGGACA AGGACAAGGA AGCACTCATG GAGGAGATCC TCAAGCTGAA GTCCCTGCTG 
AGTACCAAGC GGGAGCAGAT CACCACACTG CGCACCGTGC TCAAGGCCAA CAAGCAGACA 
GCTGAGGTGG CCCTGGCCAA CCTGAAGAGC AAGTATGAGA ACGAGAAGGC CATGGTGACC 
GAGACCATGA TGAAGCTACG GAACGAGCTC AAGGCCCTCA AGGAAGACGC AGCCACCTTC 
TCCTCCCTGC GTGCCATGTT TGCCACCAGG TGTGACGAAT ACATCACACA GCTGGATGAG 
ATGCAGCGGC AGCTGGCAGC TGCCGAGGAC GAGAAGAAGA CCCTTAACTC TCTGTTGCGC 
ATGGCCATCC AGCAGAAGCT GGCGCTCACC CAGCGGCTGG AGCTGCTGGA GCTGGACCAT 
GAACAGACCC GCAGGGGCCG CAGCAAGGCC GCCTCCAAGG CCAAGCCAGC CTCACCGAGC 
GTAAGTCACA CCTGCGCCTG CGCCAGCGAG AGGGCGGAGG GCGCCGGGCT GGCCAACCAG 
GTGTTCTGCA GCGAGAAGCA CAGCATTTAC TGTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential effects of the dynein-regulatory factor Lissencephaly-1 on processive dynein-dynactin motility.
The Journal of biological chemistry292(29)12245-12255(2017 07)
Gutierrez PA,Ackermann BE,Vershinin M,McKenney RJ

Reconstitution of the targeting of Rab6A to the Golgi apparatus in semi-intact HeLa cells: A role of BICD2 in stabilizing Rab6A on Golgi membranes and a concerted role of Rab6A/BICD2 interactions in Golgi-to-ER retrograde transport.
Biochimica et biophysica acta1853(10 Pt A)2592-609(2015 Oct)
Matsuto M,Kano F,Murata M

A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region.
eLife3e03351(2014 Oct)
Schroeder CM,Ostrem JM,Hertz NT,Vale RD

A role for Bicaudal-D2 in radial cerebellar granule cell migration.
Nature communications53411(2014 Mar)
Jaarsma D,van den Berg R,Wulf PS,van Erp S,Keijzer N,Schlager MA,de Graaff E,De Zeeuw CI,Pasterkamp RJ,Akhmanova A,Hoogenraad CC

BICD2, dynactin, and LIS1 cooperate in regulating dynein recruitment to cellular structures.
Molecular biology of the cell23(21)4226-41(2012 Nov)
Splinter D,Razafsky DS,Schlager MA,Serra-Marques A,Grigoriev I,Demmers J,Keijzer N,Jiang K,Poser I,Hyman AA,Hoogenraad CC,King SJ,Akhmanova A

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Bicd2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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