Atg2b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg2b
Entrez Gene ID	76559
Full Name	autophagy related 2B
Synonyms	2410024A21Rik,AI047755,AI503411,AW558123,C030004M05Rik,C630028L02Rik,mKIAA4067
General protein information	Preferred Names autophagy related 2B Names autophagy-related protein 2 homolog B ATG2 autophagy related 2 homolog B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 E

mRNA and Protein(s)

mRNA	Protein	Name
XM_006516360.1	XP_006516423.1	autophagy-related protein 2 homolog B isoform X1
NM_029654.4	NP_083930.5	autophagy-related protein 2 homolog B
XM_006516361.2	XP_006516424.1	autophagy-related protein 2 homolog B isoform X2
XM_006516361.3	XP_006516424.1	autophagy-related protein 2 homolog B isoform X2
XM_006516360.2	XP_006516423.1	autophagy-related protein 2 homolog B isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Atg2b	NP_083930.5
Canis lupus familiaris (dog)	ATG2B	XP_537549.3
Gallus gallus (chicken)	ATG2B	XP_421350.3
Drosophila melanogaster (fruit fly)	Atg2	NP_001261341.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004092	XP_312964.5
Xenopus tropicalis (tropical clawed frog)	atg2b	NP_001072626.1
Homo sapiens (human)	ATG2B	NP_060506.5
Bos taurus (cattle)	ATG2B	NP_001193682.1
Pan troglodytes (chimpanzee)	ATG2B	XP_510152.3
	ATG2B	XP_001101467.2

Gene Ontology
Bibliography

Related articles in PubMed

Macrophage autophagy in immunity to Cryptococcus neoformans and Candida albicans.
Nicola AM, Albuquerque P, Martinez LR, Dal-Rosso RA, Saylor C, De Jesus M, Nosanchuk JD, Casadevall A
Infection and immunity80(9)3065-76(2012 Sep)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Mouse satellite DNA is transcribed in senescent cardiac muscle.
Gaubatz JW, Cutler RG
The Journal of biological chemistry265(29)17753-8(1990 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Atg2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu48185	XM_006516361.3 Latest version!	Mus musculus autophagy related 2B (Atg2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu48184	XM_006516360.2 Latest version!	Mus musculus autophagy related 2B (Atg2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu20752	NM_029654.4 Latest version!	Mus musculus autophagy related 2B (Atg2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu48185	XM_006516361.2	Mus musculus autophagy related 2B (Atg2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu48184	XM_006516360.1	Mus musculus autophagy related 2B (Atg2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg2b ( XM_006516361.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516424.1 Mus musculus autophagy related 2B (Atg2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu48185
Clone ID Related Accession (Same CDS sequence)	XM_006516361.2 , XM_006516361.3
Accession Version	XM_006516361.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGT TCACTGATGT GAAGCAAGAG AATGGAGATC CGATGGAAAG TAAGCATGGT 
GAATTCTGGC TAGAATTCAA TAGCGGCTCG TTCTTCTGCG TGACAAAGTA TGAGGGCTTT 
GAGGACAAGC ACTACATCTG CTTGCATTCC AGCAGCCTCA GGCTGTACCA CAAAGGCATA 
GTAGATGGAG CCATTCTCCC TACAGAGACG CGCTTGCCCT GTTCCACCCG CCCACACTGG 
CTGGAACCTA CAATTTATTC CTCTGAAGAA GATGGCCTCA GCCGCACTGC CTCAGATGGG 
GTTGGGGGAG ACAATTTGAA TATGCTCTCA GTCGCAGTGA AGATACTGTC TGATAAGTCC 
GAGTCCAACA CGAAGGAGTT CCTCGTGGCT GTGGGGCTGA AAGGAGCCAC TCTACAGCAC 
AGGGTGCTGC CCGCTGGACT CAGTTGGCAC GAGCAGATTT TAAACTTCTT GAATATTGCT 
GATGAACCTG TTTTGGGATA TAACCCTCCA ACTTCATTTA CAACTTTCCA TGTCCATCTT 
TGGAGCTGTG CACTTGATTA CAGGCCCCTT CACCTGCCAC TCCGATCCCT TCTTACTGTT 
GAGACGTTCA GCATTTCTAG CAGTGTTGCT TTGGATAAAT CTTCCTCCAC TCTCAGAATC 
ATCATGGACG AGGCTGCTTT GCACCTGTCA GACAAGTGCA ACACGGTCAC TGTAAACCTG 
AATAGAGATT ATGTTCGTGT GATGGACATG GGACTTTTAG AGCTGACCAT AACCGCAGTA 
AAATCTGATT CTGATGGAGA ACAGACTGCG CCCCGCTTTG AGTTACACTG TTCCAGTGAC 
GTCGTGCACA TCAGAACATG CTCAGACTCA TGTGCTGCGC TGATGAACCT CATTCAGTAT 
GTCGCAAGTT ACGGGGACCT ACATGGACCT CACAAAGCAG AGATGAAGCC TGGAGTCCCA 
CAGAGAAAGC CCAAGGTAGA TTCCAGCGCT CGGTCATCTT CACATGGTCC AGTGCTTCCT 
GAAGCAGATC AGCAGATTTT ACGGGACCTG ATGAGTGATG CCATGGAGGA GATTGATCTG 
CAGCAGGCCT CTGCCCCTGT CGGACCACAG GCTAATGGTG TCCTGGATGA AAAGTCTCAC 
ACTCAGGAGC CGTGCTGTTC AGACCTCTTC CTGTTCCCAG ATGAGAGTGG AAATGTGTCC 
CAGGAGTCCA GCCCTGCCTA TCCGTCTCTC ACTCACCACT TGATCAGTGA CGCGGTGACC 
GGTGTGACCG CTGAAAATGA TGACTTCTGT ATTCTTTTTG CACCAAAAAC AGTCGTTCAG 
GAGAAGGAAG AAGAGCCAGT GATAAAGATC ATGGTTGACG ACGCCATTGT GATAAAAGAT 
GACTATTTCA GTCTGCCCAT CACGAGGACA GACAGCAGCA AAGCGCCGCT CCACTTTCCC 
ATCCCTGCCG TCCGATATGT TGTTAAAGAG GTCTCTCTGG TGTGGCATCT TTATGGAGGC 
AAGGACTTTG CAACAGCTCC TCCAACTTCT CCAGCTAAGA GTTACATTAG TCCCCACAGC 
TCACCGTCTC AAACACCTAC AAGGCACGGG CGCCACACAG TCTGTGGGGG CAAAGGAAGG 
AACCATGACT TCTTGATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCTCCGTGTA AGCCTGAGTG TGAGTCCAGC CTCTTAGAAC ACCCAGTCTC CAGGCAGGTC 
TTCATCGTGC AGGACCTTGA AATTCGAGAT CGGCTGGCAA CATCACAGAT GAATAAGTTT 
TTATACCTGT ATTGCAGCAA AGACATGCCT CGTAAAGCCC ATTCCAACAT GCTGACAATT 
AAAGCGTTGC ATGTACGTCC GGAGTCTGGC AGGTCACCAC AGGAGTGCTG CTTGAGAGTG 
TCCCTGATGC CTCTTCGCCT CAATATTGAC CAGGACGCCT TGTTCTTCCT CAAGGATTTC 
TTCACAAGTC TTTCTACAGA AGTGGAGCTT CTACTGACTC CAGATCCAGA AGTTACAAAG 
TCTCCTGGAG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TGAGTACCTC AAAGGAGCCA 
AATCTAGTCG TTTCTTTTCC TGGGCCAAAA CAAGCTTCCC CGAACCATCG TGCCAATTCA 
GCGGAGGGTG GTAATGGACT GGAGGAGGAC GTCTCTGCTG AGGAAACTTC ATTTAGTGAC 
CAGCCTGTGT TTTTTAGAGA ATTTAGATTC ACGGCAGAAG TTCCCATCCG GCTTGACTAC 
CACGGCAAGC ACGTGTCGAT GGATCAGGGT ACATTGGCTG GGATTTTGAT TGGCCTGGCC 
CAGTTGAACT GTTCGGAGCT AAAGCTGAAA AGGCTTTTCT ATCGGCATGG TTTGCTAGGT 
ATTGACAAAT TGTTCTCATA TGCAATCTCG GAATGGCTCA GTGACATTAA GAAGAACCAG 
CTACCAGGAA TCCTGGGAGG TGTTGGCCCT ATGCATTCAC TAGTTCAATT AGTGCAAGGC 
CTGAAGGACT TGGTATGGCT GCCCATTGAG CAATACCGGA AGGATGGCCG TATCGTCAGA 
GGCTTCCAGA GGGGCGCTGC TTCCTTTGGT ACCTCAACTG CAATGGCTGC CCTGGAACTC 
ACAAACAGAA TGGTCCAAAC CATACAGGCA GCCGCAGAGA CTGCATATGA CATGGTGTCT 
CCGAGTACCC TTTCCATTGA GCCCAAGAAG GCAAAGAGAT TTCCTCATCA CCGGTTAGCC 
CACCAGCCAG TAGACCTGAG GGAAGGCGTG GCCAAGGCCT ACAGCGTTGT AAAAGAGGGG 
ATCACAGACA CAGCTCAGAC CATCTATGAA ACGGCGGCTC GGGAACACGA GAGCAGAGGG 
GTCACTGGTG CTGTGGGTGA AGTCCTGCGG CAGATCCCAC CGGCAGTGGT CAGACCCCTG 
ATTGTAGCCA CCGAGGCGAC ATCAAATGTG TTAGGTGGCA TGAGAAACCA AATTAGACCA 
GACGTTCGGC AGGACGAGTC GCAGAAATGG CGCCATGGGG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516424.1
CDS	1387..4434
Translation	MAVFTDVKQENGDPMESKHGEFWLEFNSGSFFCVTKYEGFEDKHYICLHSSSLRLYHKGIVDGAILPTETRLPCSTRPHWLEPTIYSSEEDGLSRTASDGVGGDNLNMLSVAVKILSDKSESNTKEFLVAVGLKGATLQHRVLPAGLSWHEQILNFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLHLPLRSLLTVETFSISSSVALDKSSSTLRIIMDEAALHLSDKCNTVTVNLNRDYVRVMDMGLLELTITAVKSDSDGEQTAPRFELHCSSDVVHIRTCSDSCAALMNLIQYVASYGDLHGPHKAEMKPGVPQRKPKVDSSARSSSHGPVLPEADQQILRDLMSDAMEEIDLQQASAPVGPQANGVLDEKSHTQEPCCSDLFLFPDESGNVSQESSPAYPSLTHHLISDAVTGVTAENDDFCILFAPKTVVQEKEEEPVIKIMVDDAIVIKDDYFSLPITRTDSSKAPLHFPIPAVRYVVKEVSLVWHLYGGKDFATAPPTSPAKSYISPHSSPSQTPTRHGRHTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLLEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKDMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLLTPDPEVTKSPGADVTCSLPRHLSTSKEPNLVVSFPGPKQASPNHRANSAEGGNGLEEDVSAEETSFSDQPVFFREFRFTAEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGIDKLFSYAISEWLSDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPSTLSIEPKKAKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVRPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_006516361.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy related 2B (Atg2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516361.3

ORF Insert Sequence:

ATGGCAGTGT TCACTGATGT GAAGCAAGAG AATGGAGATC CGATGGAAAG TAAGCATGGT 
GAATTCTGGC TAGAATTCAA TAGCGGCTCG TTCTTCTGCG TGACAAAGTA TGAGGGCTTT 
GAGGACAAGC ACTACATCTG CTTGCATTCC AGCAGCCTCA GGCTGTACCA CAAAGGCATA 
GTAGATGGAG CCATTCTCCC TACAGAGACG CGCTTGCCCT GTTCCACCCG CCCACACTGG 
CTGGAACCTA CAATTTATTC CTCTGAAGAA GATGGCCTCA GCCGCACTGC CTCAGATGGG 
GTTGGGGGAG ACAATTTGAA TATGCTCTCA GTCGCAGTGA AGATACTGTC TGATAAGTCC 
GAGTCCAACA CGAAGGAGTT CCTCGTGGCT GTGGGGCTGA AAGGAGCCAC TCTACAGCAC 
AGGGTGCTGC CCGCTGGACT CAGTTGGCAC GAGCAGATTT TAAACTTCTT GAATATTGCT 
GATGAACCTG TTTTGGGATA TAACCCTCCA ACTTCATTTA CAACTTTCCA TGTCCATCTT 
TGGAGCTGTG CACTTGATTA CAGGCCCCTT CACCTGCCAC TCCGATCCCT TCTTACTGTT 
GAGACGTTCA GCATTTCTAG CAGTGTTGCT TTGGATAAAT CTTCCTCCAC TCTCAGAATC 
ATCATGGACG AGGCTGCTTT GCACCTGTCA GACAAGTGCA ACACGGTCAC TGTAAACCTG 
AATAGAGATT ATGTTCGTGT GATGGACATG GGACTTTTAG AGCTGACCAT AACCGCAGTA 
AAATCTGATT CTGATGGAGA ACAGACTGCG CCCCGCTTTG AGTTACACTG TTCCAGTGAC 
GTCGTGCACA TCAGAACATG CTCAGACTCA TGTGCTGCGC TGATGAACCT CATTCAGTAT 
GTCGCAAGTT ACGGGGACCT ACATGGACCT CACAAAGCAG AGATGAAGCC TGGAGTCCCA 
CAGAGAAAGC CCAAGGTAGA TTCCAGCGCT CGGTCATCTT CACATGGTCC AGTGCTTCCT 
GAAGCAGATC AGCAGATTTT ACGGGACCTG ATGAGTGATG CCATGGAGGA GATTGATCTG 
CAGCAGGCCT CTGCCCCTGT CGGACCACAG GCTAATGGTG TCCTGGATGA AAAGTCTCAC 
ACTCAGGAGC CGTGCTGTTC AGACCTCTTC CTGTTCCCAG ATGAGAGTGG AAATGTGTCC 
CAGGAGTCCA GCCCTGCCTA TCCGTCTCTC ACTCACCACT TGATCAGTGA CGCGGTGACC 
GGTGTGACCG CTGAAAATGA TGACTTCTGT ATTCTTTTTG CACCAAAAAC AGTCGTTCAG 
GAGAAGGAAG AAGAGCCAGT GATAAAGATC ATGGTTGACG ACGCCATTGT GATAAAAGAT 
GACTATTTCA GTCTGCCCAT CACGAGGACA GACAGCAGCA AAGCGCCGCT CCACTTTCCC 
ATCCCTGCCG TCCGATATGT TGTTAAAGAG GTCTCTCTGG TGTGGCATCT TTATGGAGGC 
AAGGACTTTG CAACAGCTCC TCCAACTTCT CCAGCTAAGA GTTACATTAG TCCCCACAGC 
TCACCGTCTC AAACACCTAC AAGGCACGGG CGCCACACAG TCTGTGGGGG CAAAGGAAGG 
AACCATGACT TCTTGATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCTCCGTGTA AGCCTGAGTG TGAGTCCAGC CTCTTAGAAC ACCCAGTCTC CAGGCAGGTC 
TTCATCGTGC AGGACCTTGA AATTCGAGAT CGGCTGGCAA CATCACAGAT GAATAAGTTT 
TTATACCTGT ATTGCAGCAA AGACATGCCT CGTAAAGCCC ATTCCAACAT GCTGACAATT 
AAAGCGTTGC ATGTACGTCC GGAGTCTGGC AGGTCACCAC AGGAGTGCTG CTTGAGAGTG 
TCCCTGATGC CTCTTCGCCT CAATATTGAC CAGGACGCCT TGTTCTTCCT CAAGGATTTC 
TTCACAAGTC TTTCTACAGA AGTGGAGCTT CTACTGACTC CAGATCCAGA AGTTACAAAG 
TCTCCTGGAG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TGAGTACCTC AAAGGAGCCA 
AATCTAGTCG TTTCTTTTCC TGGGCCAAAA CAAGCTTCCC CGAACCATCG TGCCAATTCA 
GCGGAGGGTG GTAATGGACT GGAGGAGGAC GTCTCTGCTG AGGAAACTTC ATTTAGTGAC 
CAGCCTGTGT TTTTTAGAGA ATTTAGATTC ACGGCAGAAG TTCCCATCCG GCTTGACTAC 
CACGGCAAGC ACGTGTCGAT GGATCAGGGT ACATTGGCTG GGATTTTGAT TGGCCTGGCC 
CAGTTGAACT GTTCGGAGCT AAAGCTGAAA AGGCTTTTCT ATCGGCATGG TTTGCTAGGT 
ATTGACAAAT TGTTCTCATA TGCAATCTCG GAATGGCTCA GTGACATTAA GAAGAACCAG 
CTACCAGGAA TCCTGGGAGG TGTTGGCCCT ATGCATTCAC TAGTTCAATT AGTGCAAGGC 
CTGAAGGACT TGGTATGGCT GCCCATTGAG CAATACCGGA AGGATGGCCG TATCGTCAGA 
GGCTTCCAGA GGGGCGCTGC TTCCTTTGGT ACCTCAACTG CAATGGCTGC CCTGGAACTC 
ACAAACAGAA TGGTCCAAAC CATACAGGCA GCCGCAGAGA CTGCATATGA CATGGTGTCT 
CCGAGTACCC TTTCCATTGA GCCCAAGAAG GCAAAGAGAT TTCCTCATCA CCGGTTAGCC 
CACCAGCCAG TAGACCTGAG GGAAGGCGTG GCCAAGGCCT ACAGCGTTGT AAAAGAGGGG 
ATCACAGACA CAGCTCAGAC CATCTATGAA ACGGCGGCTC GGGAACACGA GAGCAGAGGG 
GTCACTGGTG CTGTGGGTGA AGTCCTGCGG CAGATCCCAC CGGCAGTGGT CAGACCCCTG 
ATTGTAGCCA CCGAGGCGAC ATCAAATGTG TTAGGTGGCA TGAGAAACCA AATTAGACCA 
GACGTTCGGC AGGACGAGTC GCAGAAATGG CGCCATGGGG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg2b ( XM_006516361.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516424.1 Mus musculus autophagy related 2B (Atg2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu48185
Clone ID Related Accession (Same CDS sequence)	XM_006516361.2 , XM_006516361.3
Accession Version	XM_006516361.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGT TCACTGATGT GAAGCAAGAG AATGGAGATC CGATGGAAAG TAAGCATGGT 
GAATTCTGGC TAGAATTCAA TAGCGGCTCG TTCTTCTGCG TGACAAAGTA TGAGGGCTTT 
GAGGACAAGC ACTACATCTG CTTGCATTCC AGCAGCCTCA GGCTGTACCA CAAAGGCATA 
GTAGATGGAG CCATTCTCCC TACAGAGACG CGCTTGCCCT GTTCCACCCG CCCACACTGG 
CTGGAACCTA CAATTTATTC CTCTGAAGAA GATGGCCTCA GCCGCACTGC CTCAGATGGG 
GTTGGGGGAG ACAATTTGAA TATGCTCTCA GTCGCAGTGA AGATACTGTC TGATAAGTCC 
GAGTCCAACA CGAAGGAGTT CCTCGTGGCT GTGGGGCTGA AAGGAGCCAC TCTACAGCAC 
AGGGTGCTGC CCGCTGGACT CAGTTGGCAC GAGCAGATTT TAAACTTCTT GAATATTGCT 
GATGAACCTG TTTTGGGATA TAACCCTCCA ACTTCATTTA CAACTTTCCA TGTCCATCTT 
TGGAGCTGTG CACTTGATTA CAGGCCCCTT CACCTGCCAC TCCGATCCCT TCTTACTGTT 
GAGACGTTCA GCATTTCTAG CAGTGTTGCT TTGGATAAAT CTTCCTCCAC TCTCAGAATC 
ATCATGGACG AGGCTGCTTT GCACCTGTCA GACAAGTGCA ACACGGTCAC TGTAAACCTG 
AATAGAGATT ATGTTCGTGT GATGGACATG GGACTTTTAG AGCTGACCAT AACCGCAGTA 
AAATCTGATT CTGATGGAGA ACAGACTGCG CCCCGCTTTG AGTTACACTG TTCCAGTGAC 
GTCGTGCACA TCAGAACATG CTCAGACTCA TGTGCTGCGC TGATGAACCT CATTCAGTAT 
GTCGCAAGTT ACGGGGACCT ACATGGACCT CACAAAGCAG AGATGAAGCC TGGAGTCCCA 
CAGAGAAAGC CCAAGGTAGA TTCCAGCGCT CGGTCATCTT CACATGGTCC AGTGCTTCCT 
GAAGCAGATC AGCAGATTTT ACGGGACCTG ATGAGTGATG CCATGGAGGA GATTGATCTG 
CAGCAGGCCT CTGCCCCTGT CGGACCACAG GCTAATGGTG TCCTGGATGA AAAGTCTCAC 
ACTCAGGAGC CGTGCTGTTC AGACCTCTTC CTGTTCCCAG ATGAGAGTGG AAATGTGTCC 
CAGGAGTCCA GCCCTGCCTA TCCGTCTCTC ACTCACCACT TGATCAGTGA CGCGGTGACC 
GGTGTGACCG CTGAAAATGA TGACTTCTGT ATTCTTTTTG CACCAAAAAC AGTCGTTCAG 
GAGAAGGAAG AAGAGCCAGT GATAAAGATC ATGGTTGACG ACGCCATTGT GATAAAAGAT 
GACTATTTCA GTCTGCCCAT CACGAGGACA GACAGCAGCA AAGCGCCGCT CCACTTTCCC 
ATCCCTGCCG TCCGATATGT TGTTAAAGAG GTCTCTCTGG TGTGGCATCT TTATGGAGGC 
AAGGACTTTG CAACAGCTCC TCCAACTTCT CCAGCTAAGA GTTACATTAG TCCCCACAGC 
TCACCGTCTC AAACACCTAC AAGGCACGGG CGCCACACAG TCTGTGGGGG CAAAGGAAGG 
AACCATGACT TCTTGATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCTCCGTGTA AGCCTGAGTG TGAGTCCAGC CTCTTAGAAC ACCCAGTCTC CAGGCAGGTC 
TTCATCGTGC AGGACCTTGA AATTCGAGAT CGGCTGGCAA CATCACAGAT GAATAAGTTT 
TTATACCTGT ATTGCAGCAA AGACATGCCT CGTAAAGCCC ATTCCAACAT GCTGACAATT 
AAAGCGTTGC ATGTACGTCC GGAGTCTGGC AGGTCACCAC AGGAGTGCTG CTTGAGAGTG 
TCCCTGATGC CTCTTCGCCT CAATATTGAC CAGGACGCCT TGTTCTTCCT CAAGGATTTC 
TTCACAAGTC TTTCTACAGA AGTGGAGCTT CTACTGACTC CAGATCCAGA AGTTACAAAG 
TCTCCTGGAG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TGAGTACCTC AAAGGAGCCA 
AATCTAGTCG TTTCTTTTCC TGGGCCAAAA CAAGCTTCCC CGAACCATCG TGCCAATTCA 
GCGGAGGGTG GTAATGGACT GGAGGAGGAC GTCTCTGCTG AGGAAACTTC ATTTAGTGAC 
CAGCCTGTGT TTTTTAGAGA ATTTAGATTC ACGGCAGAAG TTCCCATCCG GCTTGACTAC 
CACGGCAAGC ACGTGTCGAT GGATCAGGGT ACATTGGCTG GGATTTTGAT TGGCCTGGCC 
CAGTTGAACT GTTCGGAGCT AAAGCTGAAA AGGCTTTTCT ATCGGCATGG TTTGCTAGGT 
ATTGACAAAT TGTTCTCATA TGCAATCTCG GAATGGCTCA GTGACATTAA GAAGAACCAG 
CTACCAGGAA TCCTGGGAGG TGTTGGCCCT ATGCATTCAC TAGTTCAATT AGTGCAAGGC 
CTGAAGGACT TGGTATGGCT GCCCATTGAG CAATACCGGA AGGATGGCCG TATCGTCAGA 
GGCTTCCAGA GGGGCGCTGC TTCCTTTGGT ACCTCAACTG CAATGGCTGC CCTGGAACTC 
ACAAACAGAA TGGTCCAAAC CATACAGGCA GCCGCAGAGA CTGCATATGA CATGGTGTCT 
CCGAGTACCC TTTCCATTGA GCCCAAGAAG GCAAAGAGAT TTCCTCATCA CCGGTTAGCC 
CACCAGCCAG TAGACCTGAG GGAAGGCGTG GCCAAGGCCT ACAGCGTTGT AAAAGAGGGG 
ATCACAGACA CAGCTCAGAC CATCTATGAA ACGGCGGCTC GGGAACACGA GAGCAGAGGG 
GTCACTGGTG CTGTGGGTGA AGTCCTGCGG CAGATCCCAC CGGCAGTGGT CAGACCCCTG 
ATTGTAGCCA CCGAGGCGAC ATCAAATGTG TTAGGTGGCA TGAGAAACCA AATTAGACCA 
GACGTTCGGC AGGACGAGTC GCAGAAATGG CGCCATGGGG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516424.1
CDS	663..3710
Translation	MAVFTDVKQENGDPMESKHGEFWLEFNSGSFFCVTKYEGFEDKHYICLHSSSLRLYHKGIVDGAILPTETRLPCSTRPHWLEPTIYSSEEDGLSRTASDGVGGDNLNMLSVAVKILSDKSESNTKEFLVAVGLKGATLQHRVLPAGLSWHEQILNFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLHLPLRSLLTVETFSISSSVALDKSSSTLRIIMDEAALHLSDKCNTVTVNLNRDYVRVMDMGLLELTITAVKSDSDGEQTAPRFELHCSSDVVHIRTCSDSCAALMNLIQYVASYGDLHGPHKAEMKPGVPQRKPKVDSSARSSSHGPVLPEADQQILRDLMSDAMEEIDLQQASAPVGPQANGVLDEKSHTQEPCCSDLFLFPDESGNVSQESSPAYPSLTHHLISDAVTGVTAENDDFCILFAPKTVVQEKEEEPVIKIMVDDAIVIKDDYFSLPITRTDSSKAPLHFPIPAVRYVVKEVSLVWHLYGGKDFATAPPTSPAKSYISPHSSPSQTPTRHGRHTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLLEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKDMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLLTPDPEVTKSPGADVTCSLPRHLSTSKEPNLVVSFPGPKQASPNHRANSAEGGNGLEEDVSAEETSFSDQPVFFREFRFTAEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGIDKLFSYAISEWLSDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPSTLSIEPKKAKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVRPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_006516361.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy related 2B (Atg2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516361.2

ORF Insert Sequence:

ATGGCAGTGT TCACTGATGT GAAGCAAGAG AATGGAGATC CGATGGAAAG TAAGCATGGT 
GAATTCTGGC TAGAATTCAA TAGCGGCTCG TTCTTCTGCG TGACAAAGTA TGAGGGCTTT 
GAGGACAAGC ACTACATCTG CTTGCATTCC AGCAGCCTCA GGCTGTACCA CAAAGGCATA 
GTAGATGGAG CCATTCTCCC TACAGAGACG CGCTTGCCCT GTTCCACCCG CCCACACTGG 
CTGGAACCTA CAATTTATTC CTCTGAAGAA GATGGCCTCA GCCGCACTGC CTCAGATGGG 
GTTGGGGGAG ACAATTTGAA TATGCTCTCA GTCGCAGTGA AGATACTGTC TGATAAGTCC 
GAGTCCAACA CGAAGGAGTT CCTCGTGGCT GTGGGGCTGA AAGGAGCCAC TCTACAGCAC 
AGGGTGCTGC CCGCTGGACT CAGTTGGCAC GAGCAGATTT TAAACTTCTT GAATATTGCT 
GATGAACCTG TTTTGGGATA TAACCCTCCA ACTTCATTTA CAACTTTCCA TGTCCATCTT 
TGGAGCTGTG CACTTGATTA CAGGCCCCTT CACCTGCCAC TCCGATCCCT TCTTACTGTT 
GAGACGTTCA GCATTTCTAG CAGTGTTGCT TTGGATAAAT CTTCCTCCAC TCTCAGAATC 
ATCATGGACG AGGCTGCTTT GCACCTGTCA GACAAGTGCA ACACGGTCAC TGTAAACCTG 
AATAGAGATT ATGTTCGTGT GATGGACATG GGACTTTTAG AGCTGACCAT AACCGCAGTA 
AAATCTGATT CTGATGGAGA ACAGACTGCG CCCCGCTTTG AGTTACACTG TTCCAGTGAC 
GTCGTGCACA TCAGAACATG CTCAGACTCA TGTGCTGCGC TGATGAACCT CATTCAGTAT 
GTCGCAAGTT ACGGGGACCT ACATGGACCT CACAAAGCAG AGATGAAGCC TGGAGTCCCA 
CAGAGAAAGC CCAAGGTAGA TTCCAGCGCT CGGTCATCTT CACATGGTCC AGTGCTTCCT 
GAAGCAGATC AGCAGATTTT ACGGGACCTG ATGAGTGATG CCATGGAGGA GATTGATCTG 
CAGCAGGCCT CTGCCCCTGT CGGACCACAG GCTAATGGTG TCCTGGATGA AAAGTCTCAC 
ACTCAGGAGC CGTGCTGTTC AGACCTCTTC CTGTTCCCAG ATGAGAGTGG AAATGTGTCC 
CAGGAGTCCA GCCCTGCCTA TCCGTCTCTC ACTCACCACT TGATCAGTGA CGCGGTGACC 
GGTGTGACCG CTGAAAATGA TGACTTCTGT ATTCTTTTTG CACCAAAAAC AGTCGTTCAG 
GAGAAGGAAG AAGAGCCAGT GATAAAGATC ATGGTTGACG ACGCCATTGT GATAAAAGAT 
GACTATTTCA GTCTGCCCAT CACGAGGACA GACAGCAGCA AAGCGCCGCT CCACTTTCCC 
ATCCCTGCCG TCCGATATGT TGTTAAAGAG GTCTCTCTGG TGTGGCATCT TTATGGAGGC 
AAGGACTTTG CAACAGCTCC TCCAACTTCT CCAGCTAAGA GTTACATTAG TCCCCACAGC 
TCACCGTCTC AAACACCTAC AAGGCACGGG CGCCACACAG TCTGTGGGGG CAAAGGAAGG 
AACCATGACT TCTTGATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCTCCGTGTA AGCCTGAGTG TGAGTCCAGC CTCTTAGAAC ACCCAGTCTC CAGGCAGGTC 
TTCATCGTGC AGGACCTTGA AATTCGAGAT CGGCTGGCAA CATCACAGAT GAATAAGTTT 
TTATACCTGT ATTGCAGCAA AGACATGCCT CGTAAAGCCC ATTCCAACAT GCTGACAATT 
AAAGCGTTGC ATGTACGTCC GGAGTCTGGC AGGTCACCAC AGGAGTGCTG CTTGAGAGTG 
TCCCTGATGC CTCTTCGCCT CAATATTGAC CAGGACGCCT TGTTCTTCCT CAAGGATTTC 
TTCACAAGTC TTTCTACAGA AGTGGAGCTT CTACTGACTC CAGATCCAGA AGTTACAAAG 
TCTCCTGGAG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TGAGTACCTC AAAGGAGCCA 
AATCTAGTCG TTTCTTTTCC TGGGCCAAAA CAAGCTTCCC CGAACCATCG TGCCAATTCA 
GCGGAGGGTG GTAATGGACT GGAGGAGGAC GTCTCTGCTG AGGAAACTTC ATTTAGTGAC 
CAGCCTGTGT TTTTTAGAGA ATTTAGATTC ACGGCAGAAG TTCCCATCCG GCTTGACTAC 
CACGGCAAGC ACGTGTCGAT GGATCAGGGT ACATTGGCTG GGATTTTGAT TGGCCTGGCC 
CAGTTGAACT GTTCGGAGCT AAAGCTGAAA AGGCTTTTCT ATCGGCATGG TTTGCTAGGT 
ATTGACAAAT TGTTCTCATA TGCAATCTCG GAATGGCTCA GTGACATTAA GAAGAACCAG 
CTACCAGGAA TCCTGGGAGG TGTTGGCCCT ATGCATTCAC TAGTTCAATT AGTGCAAGGC 
CTGAAGGACT TGGTATGGCT GCCCATTGAG CAATACCGGA AGGATGGCCG TATCGTCAGA 
GGCTTCCAGA GGGGCGCTGC TTCCTTTGGT ACCTCAACTG CAATGGCTGC CCTGGAACTC 
ACAAACAGAA TGGTCCAAAC CATACAGGCA GCCGCAGAGA CTGCATATGA CATGGTGTCT 
CCGAGTACCC TTTCCATTGA GCCCAAGAAG GCAAAGAGAT TTCCTCATCA CCGGTTAGCC 
CACCAGCCAG TAGACCTGAG GGAAGGCGTG GCCAAGGCCT ACAGCGTTGT AAAAGAGGGG 
ATCACAGACA CAGCTCAGAC CATCTATGAA ACGGCGGCTC GGGAACACGA GAGCAGAGGG 
GTCACTGGTG CTGTGGGTGA AGTCCTGCGG CAGATCCCAC CGGCAGTGGT CAGACCCCTG 
ATTGTAGCCA CCGAGGCGAC ATCAAATGTG TTAGGTGGCA TGAGAAACCA AATTAGACCA 
GACGTTCGGC AGGACGAGTC GCAGAAATGG CGCCATGGGG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg2b ( XM_006516360.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516423.1 Mus musculus autophagy related 2B (Atg2b), transcript variant X1, mRNA.

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CloneID	OMu48184
Clone ID Related Accession (Same CDS sequence)	XM_006516360.1 , XM_006516360.2
Accession Version	XM_006516360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CATTTTCAGA ATCCATCAAG AAGAGGGCCT GTCGCTACCT CCTACAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTGAGCCT GGATCTGTAC 
CAGGGCACCG GGTCCCTTGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAAATCCTG 
GAGTCAGCAG ATGCACCATT AGAAGTCACT GAAGGATTTA TTCAGTCCAT TTCCCTGTCC 
GTCCCATGGG GCTCCTTGCT GCAGGACAAC TGTGCCCTGG AAGTGAGAGG ACTGGAGATG 
GTCTTCCGGC CTCGACCTCG AGTAGCAACT GGGTCCGAGC CTATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAGCT GGCAAAGGAG TGCCTTAGCC AGAAACTGAC AGATGAGCAA 
GGAGAGGCAT CCCAACCTTT CGAGGGACTG GAAAAGTTTG CAGAAACCAT CGAAACAGTA 
CTAAGAAGAG TGAAAGTCAC TTTTATAGAT ACTGTTTTAA GAATTGAACA TGTGCCAGAA 
AATTCTAAAA CTGGAGCTGC ACTTGAAATC CGAGTAGACA GGACCGTGTA CTGTGACGAA 
ACAGCCGACG AGTCCTCAGG AGTCAATGTG CATCAGCCCA CAGCTTTTGC TCATAAATTG 
CTGCAGCTCT CTGGGGTGTC TCTGTTCTGG GATGAGTTTT CTGCTTCAGC AAAATCTTCC 
CCCGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAACCCC 
AAAATTGTTT ATGAGCCACA CCCACAACTA ACTAGAACGT TACCGGAGAT CGCACCCTCG 
GATCCAGTGC AGATTGGAAG GCTACTGGGC AGGCTGGAGT TGAGTCTCAC TTTAAAACAG 
AATGAAGTGC TTCCAGGAGC CAAGTTGGAT GTCGATGGAC AGATAGACTC CTTTCATCTA 
TTCCTGTCAC CGAGGCAGGT ACACCTCCTT CTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GCTGGCTAAT AAAGACAGGA AAAATCGACC CATGCAGCAG 
GAAGATGAGT ACAGAATTCA GATGGAATTA AACCGGTATT ATCTGAGGAA AGATTCCCTC 
TCTATGGGTG TGTCTTCTGA GAAGAGCTTC TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAGG AAGAAGTTTT CTTCTCCATG GCCGACATGG ACATGTCTCA CAGTCTGTCC 
TCTCTTCCAC CCCTTGGGGA CCCACCACAC ATGGACCTGG AGCTGTCGCT GACGAGTACA 
TACACCAACA CACCCGCAGG GTCCCCGCTA AGCGCCACTG TGCTTCAGCC AACTTGGGGA 
GAGTTTGCCG ACCACAAAGA ACAGCCAGTC AGAGGGCCAG CATTCCAGTC TGACGTGGTG 
CACCCGGCAT CTTTACAAAA AGCTGCTCTC TCCTCTAGGT CTGCTTCAGT GGATGAATCC 
AGACCTGAGT TTATCTGCAG GCTGGCTCTC GGCATATTCT CCGTCTCCGT GCTTCACATC 
GATCCTTTGT CTCCAGCGGA AACGTCTCTG AACGTCAACC CATTGACACG CATGGCCACC 
GATTTCTTCT CCTGTGTAGA AAAGATGGAC CCAGCAATAT TTTCGACGGG AGACTTTAAG 
TCTTTCCGAG CAGTCTTTGC CGAAGCTTGT TCACACGATC ACCTTAGGTT TATAGGTACT 
GGCATCAAAG TGTCCTATGA GCAGAGACAA AGGTCAGCTT CCCGGCACTT TAGCACCGAC 
ATGTCCGTTG GGCAAATGGA GTTTTTGGAG TGTCTGTTTC CAACAGATTG TCATTCTGTC 
CCTTCTCACT ACACCGAGCT TCTAACGTTC CATTCCAAAG AAGGGACGGA TGCCCACCTT 
CCCGTTTGTC TTCAGCTTCA CTATAAACAC TCAGAGACTA GAGGACCCCA GGGTAATCAA 
GCAAGACTTA GTTCAGTTCC TCAAAAGGCA GAATTACAAA TTAAATTAAA CCCCGTGTGT 
TGCGAGCTGG ATATCAGTAT TGTGGATAGG TTAAATTCCT TGCTTCAACC TCAGAAACTC 
ACTACCGTGG AGATGATGGC CTCGCACATG TATGCGTCCT ATAACAAGCA TATTAGTTTG 
CACAAGGCTT TCACGGAAGT GTTCCTAGAT GACTCACACA GTCCTGCAAA CCGTCGGGTG 
TCCGTACAAG TTGCCACCCC AGCCCTGCAC CTGTCTGTTC GCTTCCCTAT TCCTGATCTA 
AGATCAGACC AGGAAAGAGG ACCCTGGTTT AAGAAGTCAC TTCAGAAGGA GACCCTTCAT 
TTAGAATTTA CGGATCTGGA GTCCAAGACA GAGTTCGTGG GAGGATCCAC CCCAGAACAA 
ACTAAACTGG AGCTGACTTT TCGAGAGCTA TCTGGGTCGT TCCAGGAGGA GAAGGGAGGG 
CCATCTGTCA AGTTCTTCCA TGTGTCTGGT GGAGTAGATG GAGACACCGC ATCGTCAGAT 
GACTTTGACT GGCCACGAAT GGTACTGAAA ATAAACCCAC CAGCCATGCA TTCCATTTTG 
GAGAGAATAG CAGCCGAGGA AGAGGAAGAG AATGATGGTC ACTACCAGGA GGAGGAGGAC 
GGAGGGGCTC ATTCCCTGAA AGATGTCTGT GACCTGAGGC GGCCAGCCCC GTCTCCCTTT 
TCCTCTCGTC GAGTGATGTT TGAGAATGAG CAGATGGTGA TGCCGGGAGA CCCTGTGGAG 
ATGACGGAGT TTCAGGATAA AGCCATCAGC AACTCGCACT ACGTGCTGGA GCTCCTGTTG 
CCCAACATCC ACCTGACGCT GCCCAACAAA GGCTTCTACG AGAAGCTCTA TAACAGGATC 
TTTAACGACT TGCTTCTCTG GGAGCCAACA GCGCCTTCAC CAGTGGAGAC ACTTGAAAAC 
GTTTCCTATG GCATCGGACT CTCCGTGGCC AGTCAGCTGA TCAATACCTT CAGCAAAGAT 
AGCTTCAGTC CCTTTAAATC TGCAGTTCAC TATGATGAAG ACAGTGGATC TGAAGAAGAG 
ACTTTGCAGT ATTTTTCTGC TGTTGATCCC AACTATCGTT CCCGTAGGAA AAAAAAGTTG 
GACTCTCAGA ACAAGAACTC TCAGAGCTTC CTCTCAGTGC TTCTGAGTAT CAATCATGGA 
CTAATGGCAG TGTTCACTGA TGTGAAGCAA GAGAATGGAG ATCCGATGGA AAGTAAGCAT 
GGTGAATTCT GGCTAGAATT CAATAGCGGC TCGTTCTTCT GCGTGACAAA GTATGAGGGC 
TTTGAGGACA AGCACTACAT CTGCTTGCAT TCCAGCAGCC TCAGGCTGTA CCACAAAGGC 
ATAGTAGATG GAGCCATTCT CCCTACAGAG ACGCGCTTGC CCTGTTCCAC CCGCCCACAC 
TGGCTGGAAC CTACAATTTA TTCCTCTGAA GAAGATGGCC TCAGCCGCAC TGCCTCAGAT 
GGGGTTGGGG GAGACAATTT GAATATGCTC TCAGTCGCAG TGAAGATACT GTCTGATAAG 
TCCGAGTCCA ACACGAAGGA GTTCCTCGTG GCTGTGGGGC TGAAAGGAGC CACTCTACAG 
CACAGGGTGC TGCCCGCTGG ACTCAGTTGG CACGAGCAGA TTTTAAACTT CTTGAATATT 
GCTGATGAAC CTGTTTTGGG ATATAACCCT CCAACTTCAT TTACAACTTT CCATGTCCAT 
CTTTGGAGCT GTGCACTTGA TTACAGGCCC CTTCACCTGC CACTCCGATC CCTTCTTACT 
GTTGAGACGT TCAGCATTTC TAGCAGTGTT GCTTTGGATA AATCTTCCTC CACTCTCAGA 
ATCATCATGG ACGAGGCTGC TTTGCACCTG TCAGACAAGT GCAACACGGT CACTGTAAAC 
CTGAATAGAG ATTATGTTCG TGTGATGGAC ATGGGACTTT TAGAGCTGAC CATAACCGCA 
GTAAAATCTG ATTCTGATGG AGAACAGACT GCGCCCCGCT TTGAGTTACA CTGTTCCAGT 
GACGTCGTGC ACATCAGAAC ATGCTCAGAC TCATGTGCTG CGCTGATGAA CCTCATTCAG 
TATGTCGCAA GTTACGGGGA CCTACATGGA CCTCACAAAG CAGAGATGAA GCCTGGAGTC 
CCACAGAGAA AGCCCAAGGT AGATTCCAGC GCTCGGTCAT CTTCACATGG TCCAGTGCTT 
CCTGAAGCAG ATCAGCAGAT TTTACGGGAC CTGATGAGTG ATGCCATGGA GGAGATTGAT 
CTGCAGCAGG CCTCTGCCCC TGTCGGACCA CAGGCTAATG GTGTCCTGGA TGAAAAGTCT 
CACACTCAGG AGCCGTGCTG TTCAGACCTC TTCCTGTTCC CAGATGAGAG TGGAAATGTG 
TCCCAGGAGT CCAGCCCTGC CTATCCGTCT CTCACTCACC ACTTGATCAG TGACGCGGTG 
ACCGGTGTGA CCGCTGAAAA TGATGACTTC TGTATTCTTT TTGCACCAAA AACAGTCGTT 
CAGGAGAAGG AAGAAGAGCC AGTGATAAAG ATCATGGTTG ACGACGCCAT TGTGATAAAA 
GATGACTATT TCAGTCTGCC CATCACGAGG ACAGACAGCA GCAAAGCGCC GCTCCACTTT 
CCCATCCCTG CCGTCCGATA TGTTGTTAAA GAGGTCTCTC TGGTGTGGCA TCTTTATGGA 
GGCAAGGACT TTGCAACAGC TCCTCCAACT TCTCCAGCTA AGAGTTACAT TAGTCCCCAC 
AGCTCACCGT CTCAAACACC TACAAGGCAC GGGCGCCACA CAGTCTGTGG GGGCAAAGGA 
AGGAACCATG ACTTCTTGAT GGAAATACAG TTAAGCAAGG TGAAGTTTCA GCATGAGGTC 
TACCCTCCGT GTAAGCCTGA GTGTGAGTCC AGCCTCTTAG AACACCCAGT CTCCAGGCAG 
GTCTTCATCG TGCAGGACCT TGAAATTCGA GATCGGCTGG CAACATCACA GATGAATAAG 
TTTTTATACC TGTATTGCAG CAAAGACATG CCTCGTAAAG CCCATTCCAA CATGCTGACA 
ATTAAAGCGT TGCATGTACG TCCGGAGTCT GGCAGGTCAC CACAGGAGTG CTGCTTGAGA 
GTGTCCCTGA TGCCTCTTCG CCTCAATATT GACCAGGACG CCTTGTTCTT CCTCAAGGAT 
TTCTTCACAA GTCTTTCTAC AGAAGTGGAG CTTCTACTGA CTCCAGATCC AGAAGTTACA 
AAGTCTCCTG GAGCTGATGT CACCTGCAGT TTGCCAAGGC ATTTGAGTAC CTCAAAGGAG 
CCAAATCTAG TCGTTTCTTT TCCTGGGCCA AAACAAGCTT CCCCGAACCA TCGTGCCAAT 
TCAGCGGAGG GTGGTAATGG ACTGGAGGAG GACGTCTCTG CTGAGGAAAC TTCATTTAGT 
GACCAGCCTG TGTTTTTTAG AGAATTTAGA TTCACGGCAG AAGTTCCCAT CCGGCTTGAC 
TACCACGGCA AGCACGTGTC GATGGATCAG GGTACATTGG CTGGGATTTT GATTGGCCTG 
GCCCAGTTGA ACTGTTCGGA GCTAAAGCTG AAAAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTATTGACA AATTGTTCTC ATATGCAATC TCGGAATGGC TCAGTGACAT TAAGAAGAAC 
CAGCTACCAG GAATCCTGGG AGGTGTTGGC CCTATGCATT CACTAGTTCA ATTAGTGCAA 
GGCCTGAAGG ACTTGGTATG GCTGCCCATT GAGCAATACC GGAAGGATGG CCGTATCGTC 
AGAGGCTTCC AGAGGGGCGC TGCTTCCTTT GGTACCTCAA CTGCAATGGC TGCCCTGGAA 
CTCACAAACA GAATGGTCCA AACCATACAG GCAGCCGCAG AGACTGCATA TGACATGGTG 
TCTCCGAGTA CCCTTTCCAT TGAGCCCAAG AAGGCAAAGA GATTTCCTCA TCACCGGTTA 
GCCCACCAGC CAGTAGACCT GAGGGAAGGC GTGGCCAAGG CCTACAGCGT TGTAAAAGAG 
GGGATCACAG ACACAGCTCA GACCATCTAT GAAACGGCGG CTCGGGAACA CGAGAGCAGA 
GGGGTCACTG GTGCTGTGGG TGAAGTCCTG CGGCAGATCC CACCGGCAGT GGTCAGACCC 
CTGATTGTAG CCACCGAGGC GACATCAAAT GTGTTAGGTG GCATGAGAAA CCAAATTAGA 
CCAGACGTTC GGCAGGACGA GTCGCAGAAA TGGCGCCATG GGGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516423.1
CDS	468..6698
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRVATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEASQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGAALEIRVDRTVYCDETADESSGVNVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRTLPEIAPSDPVQIGRLLGRLELSLTLKQNEVLPGAKLDVDGQIDSFHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSMGVSSEKSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPHMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFADHKEQPVRGPAFQSDVVHPASLQKAALSSRSASVDESRPEFICRLALGIFSVSVLHIDPLSPAETSLNVNPLTRMATDFFSCVEKMDPAIFSTGDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRHFSTDMSVGQMEFLECLFPTDCHSVPSHYTELLTFHSKEGTDAHLPVCLQLHYKHSETRGPQGNQARLSSVPQKAELQIKLNPVCCELDISIVDRLNSLLQPQKLTTVEMMASHMYASYNKHISLHKAFTEVFLDDSHSPANRRVSVQVATPALHLSVRFPIPDLRSDQERGPWFKKSLQKETLHLEFTDLESKTEFVGGSTPEQTKLELTFRELSGSFQEEKGGPSVKFFHVSGGVDGDTASSDDFDWPRMVLKINPPAMHSILERIAAEEEEENDGHYQEEEDGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELLLPNIHLTLPNKGFYEKLYNRIFNDLLLWEPTAPSPVETLENVSYGIGLSVASQLINTFSKDSFSPFKSAVHYDEDSGSEEETLQYFSAVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVKQENGDPMESKHGEFWLEFNSGSFFCVTKYEGFEDKHYICLHSSSLRLYHKGIVDGAILPTETRLPCSTRPHWLEPTIYSSEEDGLSRTASDGVGGDNLNMLSVAVKILSDKSESNTKEFLVAVGLKGATLQHRVLPAGLSWHEQILNFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLHLPLRSLLTVETFSISSSVALDKSSSTLRIIMDEAALHLSDKCNTVTVNLNRDYVRVMDMGLLELTITAVKSDSDGEQTAPRFELHCSSDVVHIRTCSDSCAALMNLIQYVASYGDLHGPHKAEMKPGVPQRKPKVDSSARSSSHGPVLPEADQQILRDLMSDAMEEIDLQQASAPVGPQANGVLDEKSHTQEPCCSDLFLFPDESGNVSQESSPAYPSLTHHLISDAVTGVTAENDDFCILFAPKTVVQEKEEEPVIKIMVDDAIVIKDDYFSLPITRTDSSKAPLHFPIPAVRYVVKEVSLVWHLYGGKDFATAPPTSPAKSYISPHSSPSQTPTRHGRHTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLLEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKDMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLLTPDPEVTKSPGADVTCSLPRHLSTSKEPNLVVSFPGPKQASPNHRANSAEGGNGLEEDVSAEETSFSDQPVFFREFRFTAEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGIDKLFSYAISEWLSDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPSTLSIEPKKAKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVRPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_006516360.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy related 2B (Atg2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516360.2

ORF Insert Sequence:

ATGCCTTGGC CATTTTCAGA ATCCATCAAG AAGAGGGCCT GTCGCTACCT CCTACAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTGAGCCT GGATCTGTAC 
CAGGGCACCG GGTCCCTTGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAAATCCTG 
GAGTCAGCAG ATGCACCATT AGAAGTCACT GAAGGATTTA TTCAGTCCAT TTCCCTGTCC 
GTCCCATGGG GCTCCTTGCT GCAGGACAAC TGTGCCCTGG AAGTGAGAGG ACTGGAGATG 
GTCTTCCGGC CTCGACCTCG AGTAGCAACT GGGTCCGAGC CTATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAGCT GGCAAAGGAG TGCCTTAGCC AGAAACTGAC AGATGAGCAA 
GGAGAGGCAT CCCAACCTTT CGAGGGACTG GAAAAGTTTG CAGAAACCAT CGAAACAGTA 
CTAAGAAGAG TGAAAGTCAC TTTTATAGAT ACTGTTTTAA GAATTGAACA TGTGCCAGAA 
AATTCTAAAA CTGGAGCTGC ACTTGAAATC CGAGTAGACA GGACCGTGTA CTGTGACGAA 
ACAGCCGACG AGTCCTCAGG AGTCAATGTG CATCAGCCCA CAGCTTTTGC TCATAAATTG 
CTGCAGCTCT CTGGGGTGTC TCTGTTCTGG GATGAGTTTT CTGCTTCAGC AAAATCTTCC 
CCCGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAACCCC 
AAAATTGTTT ATGAGCCACA CCCACAACTA ACTAGAACGT TACCGGAGAT CGCACCCTCG 
GATCCAGTGC AGATTGGAAG GCTACTGGGC AGGCTGGAGT TGAGTCTCAC TTTAAAACAG 
AATGAAGTGC TTCCAGGAGC CAAGTTGGAT GTCGATGGAC AGATAGACTC CTTTCATCTA 
TTCCTGTCAC CGAGGCAGGT ACACCTCCTT CTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GCTGGCTAAT AAAGACAGGA AAAATCGACC CATGCAGCAG 
GAAGATGAGT ACAGAATTCA GATGGAATTA AACCGGTATT ATCTGAGGAA AGATTCCCTC 
TCTATGGGTG TGTCTTCTGA GAAGAGCTTC TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAGG AAGAAGTTTT CTTCTCCATG GCCGACATGG ACATGTCTCA CAGTCTGTCC 
TCTCTTCCAC CCCTTGGGGA CCCACCACAC ATGGACCTGG AGCTGTCGCT GACGAGTACA 
TACACCAACA CACCCGCAGG GTCCCCGCTA AGCGCCACTG TGCTTCAGCC AACTTGGGGA 
GAGTTTGCCG ACCACAAAGA ACAGCCAGTC AGAGGGCCAG CATTCCAGTC TGACGTGGTG 
CACCCGGCAT CTTTACAAAA AGCTGCTCTC TCCTCTAGGT CTGCTTCAGT GGATGAATCC 
AGACCTGAGT TTATCTGCAG GCTGGCTCTC GGCATATTCT CCGTCTCCGT GCTTCACATC 
GATCCTTTGT CTCCAGCGGA AACGTCTCTG AACGTCAACC CATTGACACG CATGGCCACC 
GATTTCTTCT CCTGTGTAGA AAAGATGGAC CCAGCAATAT TTTCGACGGG AGACTTTAAG 
TCTTTCCGAG CAGTCTTTGC CGAAGCTTGT TCACACGATC ACCTTAGGTT TATAGGTACT 
GGCATCAAAG TGTCCTATGA GCAGAGACAA AGGTCAGCTT CCCGGCACTT TAGCACCGAC 
ATGTCCGTTG GGCAAATGGA GTTTTTGGAG TGTCTGTTTC CAACAGATTG TCATTCTGTC 
CCTTCTCACT ACACCGAGCT TCTAACGTTC CATTCCAAAG AAGGGACGGA TGCCCACCTT 
CCCGTTTGTC TTCAGCTTCA CTATAAACAC TCAGAGACTA GAGGACCCCA GGGTAATCAA 
GCAAGACTTA GTTCAGTTCC TCAAAAGGCA GAATTACAAA TTAAATTAAA CCCCGTGTGT 
TGCGAGCTGG ATATCAGTAT TGTGGATAGG TTAAATTCCT TGCTTCAACC TCAGAAACTC 
ACTACCGTGG AGATGATGGC CTCGCACATG TATGCGTCCT ATAACAAGCA TATTAGTTTG 
CACAAGGCTT TCACGGAAGT GTTCCTAGAT GACTCACACA GTCCTGCAAA CCGTCGGGTG 
TCCGTACAAG TTGCCACCCC AGCCCTGCAC CTGTCTGTTC GCTTCCCTAT TCCTGATCTA 
AGATCAGACC AGGAAAGAGG ACCCTGGTTT AAGAAGTCAC TTCAGAAGGA GACCCTTCAT 
TTAGAATTTA CGGATCTGGA GTCCAAGACA GAGTTCGTGG GAGGATCCAC CCCAGAACAA 
ACTAAACTGG AGCTGACTTT TCGAGAGCTA TCTGGGTCGT TCCAGGAGGA GAAGGGAGGG 
CCATCTGTCA AGTTCTTCCA TGTGTCTGGT GGAGTAGATG GAGACACCGC ATCGTCAGAT 
GACTTTGACT GGCCACGAAT GGTACTGAAA ATAAACCCAC CAGCCATGCA TTCCATTTTG 
GAGAGAATAG CAGCCGAGGA AGAGGAAGAG AATGATGGTC ACTACCAGGA GGAGGAGGAC 
GGAGGGGCTC ATTCCCTGAA AGATGTCTGT GACCTGAGGC GGCCAGCCCC GTCTCCCTTT 
TCCTCTCGTC GAGTGATGTT TGAGAATGAG CAGATGGTGA TGCCGGGAGA CCCTGTGGAG 
ATGACGGAGT TTCAGGATAA AGCCATCAGC AACTCGCACT ACGTGCTGGA GCTCCTGTTG 
CCCAACATCC ACCTGACGCT GCCCAACAAA GGCTTCTACG AGAAGCTCTA TAACAGGATC 
TTTAACGACT TGCTTCTCTG GGAGCCAACA GCGCCTTCAC CAGTGGAGAC ACTTGAAAAC 
GTTTCCTATG GCATCGGACT CTCCGTGGCC AGTCAGCTGA TCAATACCTT CAGCAAAGAT 
AGCTTCAGTC CCTTTAAATC TGCAGTTCAC TATGATGAAG ACAGTGGATC TGAAGAAGAG 
ACTTTGCAGT ATTTTTCTGC TGTTGATCCC AACTATCGTT CCCGTAGGAA AAAAAAGTTG 
GACTCTCAGA ACAAGAACTC TCAGAGCTTC CTCTCAGTGC TTCTGAGTAT CAATCATGGA 
CTAATGGCAG TGTTCACTGA TGTGAAGCAA GAGAATGGAG ATCCGATGGA AAGTAAGCAT 
GGTGAATTCT GGCTAGAATT CAATAGCGGC TCGTTCTTCT GCGTGACAAA GTATGAGGGC 
TTTGAGGACA AGCACTACAT CTGCTTGCAT TCCAGCAGCC TCAGGCTGTA CCACAAAGGC 
ATAGTAGATG GAGCCATTCT CCCTACAGAG ACGCGCTTGC CCTGTTCCAC CCGCCCACAC 
TGGCTGGAAC CTACAATTTA TTCCTCTGAA GAAGATGGCC TCAGCCGCAC TGCCTCAGAT 
GGGGTTGGGG GAGACAATTT GAATATGCTC TCAGTCGCAG TGAAGATACT GTCTGATAAG 
TCCGAGTCCA ACACGAAGGA GTTCCTCGTG GCTGTGGGGC TGAAAGGAGC CACTCTACAG 
CACAGGGTGC TGCCCGCTGG ACTCAGTTGG CACGAGCAGA TTTTAAACTT CTTGAATATT 
GCTGATGAAC CTGTTTTGGG ATATAACCCT CCAACTTCAT TTACAACTTT CCATGTCCAT 
CTTTGGAGCT GTGCACTTGA TTACAGGCCC CTTCACCTGC CACTCCGATC CCTTCTTACT 
GTTGAGACGT TCAGCATTTC TAGCAGTGTT GCTTTGGATA AATCTTCCTC CACTCTCAGA 
ATCATCATGG ACGAGGCTGC TTTGCACCTG TCAGACAAGT GCAACACGGT CACTGTAAAC 
CTGAATAGAG ATTATGTTCG TGTGATGGAC ATGGGACTTT TAGAGCTGAC CATAACCGCA 
GTAAAATCTG ATTCTGATGG AGAACAGACT GCGCCCCGCT TTGAGTTACA CTGTTCCAGT 
GACGTCGTGC ACATCAGAAC ATGCTCAGAC TCATGTGCTG CGCTGATGAA CCTCATTCAG 
TATGTCGCAA GTTACGGGGA CCTACATGGA CCTCACAAAG CAGAGATGAA GCCTGGAGTC 
CCACAGAGAA AGCCCAAGGT AGATTCCAGC GCTCGGTCAT CTTCACATGG TCCAGTGCTT 
CCTGAAGCAG ATCAGCAGAT TTTACGGGAC CTGATGAGTG ATGCCATGGA GGAGATTGAT 
CTGCAGCAGG CCTCTGCCCC TGTCGGACCA CAGGCTAATG GTGTCCTGGA TGAAAAGTCT 
CACACTCAGG AGCCGTGCTG TTCAGACCTC TTCCTGTTCC CAGATGAGAG TGGAAATGTG 
TCCCAGGAGT CCAGCCCTGC CTATCCGTCT CTCACTCACC ACTTGATCAG TGACGCGGTG 
ACCGGTGTGA CCGCTGAAAA TGATGACTTC TGTATTCTTT TTGCACCAAA AACAGTCGTT 
CAGGAGAAGG AAGAAGAGCC AGTGATAAAG ATCATGGTTG ACGACGCCAT TGTGATAAAA 
GATGACTATT TCAGTCTGCC CATCACGAGG ACAGACAGCA GCAAAGCGCC GCTCCACTTT 
CCCATCCCTG CCGTCCGATA TGTTGTTAAA GAGGTCTCTC TGGTGTGGCA TCTTTATGGA 
GGCAAGGACT TTGCAACAGC TCCTCCAACT TCTCCAGCTA AGAGTTACAT TAGTCCCCAC 
AGCTCACCGT CTCAAACACC TACAAGGCAC GGGCGCCACA CAGTCTGTGG GGGCAAAGGA 
AGGAACCATG ACTTCTTGAT GGAAATACAG TTAAGCAAGG TGAAGTTTCA GCATGAGGTC 
TACCCTCCGT GTAAGCCTGA GTGTGAGTCC AGCCTCTTAG AACACCCAGT CTCCAGGCAG 
GTCTTCATCG TGCAGGACCT TGAAATTCGA GATCGGCTGG CAACATCACA GATGAATAAG 
TTTTTATACC TGTATTGCAG CAAAGACATG CCTCGTAAAG CCCATTCCAA CATGCTGACA 
ATTAAAGCGT TGCATGTACG TCCGGAGTCT GGCAGGTCAC CACAGGAGTG CTGCTTGAGA 
GTGTCCCTGA TGCCTCTTCG CCTCAATATT GACCAGGACG CCTTGTTCTT CCTCAAGGAT 
TTCTTCACAA GTCTTTCTAC AGAAGTGGAG CTTCTACTGA CTCCAGATCC AGAAGTTACA 
AAGTCTCCTG GAGCTGATGT CACCTGCAGT TTGCCAAGGC ATTTGAGTAC CTCAAAGGAG 
CCAAATCTAG TCGTTTCTTT TCCTGGGCCA AAACAAGCTT CCCCGAACCA TCGTGCCAAT 
TCAGCGGAGG GTGGTAATGG ACTGGAGGAG GACGTCTCTG CTGAGGAAAC TTCATTTAGT 
GACCAGCCTG TGTTTTTTAG AGAATTTAGA TTCACGGCAG AAGTTCCCAT CCGGCTTGAC 
TACCACGGCA AGCACGTGTC GATGGATCAG GGTACATTGG CTGGGATTTT GATTGGCCTG 
GCCCAGTTGA ACTGTTCGGA GCTAAAGCTG AAAAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTATTGACA AATTGTTCTC ATATGCAATC TCGGAATGGC TCAGTGACAT TAAGAAGAAC 
CAGCTACCAG GAATCCTGGG AGGTGTTGGC CCTATGCATT CACTAGTTCA ATTAGTGCAA 
GGCCTGAAGG ACTTGGTATG GCTGCCCATT GAGCAATACC GGAAGGATGG CCGTATCGTC 
AGAGGCTTCC AGAGGGGCGC TGCTTCCTTT GGTACCTCAA CTGCAATGGC TGCCCTGGAA 
CTCACAAACA GAATGGTCCA AACCATACAG GCAGCCGCAG AGACTGCATA TGACATGGTG 
TCTCCGAGTA CCCTTTCCAT TGAGCCCAAG AAGGCAAAGA GATTTCCTCA TCACCGGTTA 
GCCCACCAGC CAGTAGACCT GAGGGAAGGC GTGGCCAAGG CCTACAGCGT TGTAAAAGAG 
GGGATCACAG ACACAGCTCA GACCATCTAT GAAACGGCGG CTCGGGAACA CGAGAGCAGA 
GGGGTCACTG GTGCTGTGGG TGAAGTCCTG CGGCAGATCC CACCGGCAGT GGTCAGACCC 
CTGATTGTAG CCACCGAGGC GACATCAAAT GTGTTAGGTG GCATGAGAAA CCAAATTAGA 
CCAGACGTTC GGCAGGACGA GTCGCAGAAA TGGCGCCATG GGGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg2b ( NM_029654.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083930.5 Mus musculus autophagy related 2B (Atg2b), mRNA.

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CloneID	OMu20752
Clone ID Related Accession (Same CDS sequence)	NM_029654.4
Accession Version	NM_029654.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	autophagy-related protein 2 homolog B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK086802.1, BC062182.1, CA751117.1 and AC068459.9. On or before Nov 21, 2006 this sequence version replaced NM_176899.2, NM_029654.3. ##Evidence-Data-START## Transcript exon combination :: BC062182.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTTGGC CATTTTCAGA ATCCATCAAG AAGAGGGCCT GTCGCTACCT CCTACAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTGAGCCT GGATCTGTAC 
CAGGGCACCG GGTCCCTTGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAAATCCTG 
GAGTCAGCAG ATGCACCATT AGAAGTCACT GAAGGATTTA TTCAGTCCAT TTCCCTGTCC 
GTCCCATGGG GCTCCTTGCT GCAGGACAAC TGTGCCCTGG AAGTGAGAGG ACTGGAGATG 
GTCTTCCGGC CTCGACCTCG AGTAGCAACT GGGTCCGAGC CTATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAGCT GGCAAAGGAG TGCCTTAGCC AGAAACTGAC AGATGAGCAA 
GGAGAGGCAT CCCAACCTTT CGAGGGACTG GAAAAGTTTG CAGAAACCAT CGAAACAGTA 
CTAAGAAGAG TGAAAGTCAC TTTTATAGAT ACTGTTTTAA GAATTGAACA TGTGCCAGAA 
AATTCTAAAA CTGGAGCTGC ACTTGAAATC CGAGTAGACA GGACCGTGTA CTGTGACGAA 
ACAGCCGACG AGTCCTCAGG AGTCAATGTG CATCAGCCCA CAGCTTTTGC TCATAAATTG 
CTGCAGCTCT CTGGGGTGTC TCTGTTCTGG GATGAGTTTT CTGCTTCAGC AAAATCTTCC 
CCCGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAACCCC 
AAAATTGTTT ATGAGCCACA CCCACAACTA ACTAGAACGT TACCGGAGAT CGCACCCTCG 
GATCCAGTGC AGATTGGAAG GCTACTGGGC AGGCTGGAGT TGAGTCTCAC TTTAAAACAG 
AATGAAGTGC TTCCAGGAGC CAAGTTGGAT GTCGATGGAC AGATAGACTC CTTTCATCTA 
TTCCTGTCAC CGAGGCAGGT ACACCTCCTT CTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GCTGGCTAAT AAAGACAGGA AAAATCGACC CATGCAGCAG 
GAAGATGAGT ACAGAATTCA GATGGAATTA AACCGGTATT ATCTGAGGAA AGATTCCCTC 
TCTATGGGTG TGTCTTCTGA GAAGAGCTTC TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAGG AAGAAGTTTT CTTCTCCATG GCCGACATGG ACATGTCTCA CAGTCTGTCC 
TCTCTTCCAC CCCTTGGGGA CCCACCACAC ATGGACCTGG AGCTGTCGCT GACGAGTACA 
TACACCAACA CACCCGCAGG GTCCCCGCTA AGCGCCACTG TGCTTCAGCC AACTTGGGGA 
GAGTTTGCCG ACCACAAAGA ACAGCCAGTC AGAGGGCCAG CATTCCAGTC TGACGTGGTG 
CACCCGGCAT CTTTACAAAA AGCTGCTCTC TCCTCTAGGT CTGCTTCAGT GGATGAATCC 
AGACCTGAGT TTATCTGCAG GCTGGCTCTC GGCATATTCT CCGTCTCCGT GCTTCACATC 
GATCCTTTGT CTCCAGCGGA AACGTCTCTG AACGTCAACC CATTGACACG CATGGCCACC 
GATTTCTTCT CCTGTGTAGA AAAGATGGAC CCAGCAATAT TTTCGACGGG AGACTTTAAG 
TCTTTCCGAG CAGTCTTTGC CGAAGCTTGT TCACACGATC ACCTTAGGTT TATAGGTACT 
GGCATCAAAG TGTCCTATGA GCAGAGACAA AGGTCAGCTT CCCGGCACTT TAGCACCGAC 
ATGTCCGTTG GGCAAATGGA GTTTTTGGAG TGTCTGTTTC CAACAGATTG TCATTCTGTC 
CCTTCTCACT ACACCGAGCT TCTAACGTTC CATTCCAAAG AAGGGACGGA TGCCCACCTT 
CCCGTTTGTC TTCAGCTTCA CTATAAACAC TCAGAGACTA GAGGACCCCA GGGTAATCAA 
GCAAGACTTA GTTCAGTTCC TCAAAAGGCA GAATTACAAA TTAAATTAAA CCCCGTGTGT 
TGCGAGCTGG ATATCAGTAT TGTGGATAGG TTAAATTCCT TGCTTCAACC TCAGAAACTC 
ACTACCGTGG AGATGATGGC CTCGCACATG TATGCGTCCT ATAACAAGCA TATTAGTTTG 
CACAAGGCTT TCACGGAAGT GTTCCTAGAT GACTCACACA GTCCTGCAAA CCGTCGGGTG 
TCCGTACAAG TTGCCACCCC AGCCCTGCAC CTGTCTGTTC GCTTCCCTAT TCCTGATCTA 
AGATCAGACC AGGAAAGAGG ACCCTGGTTT AAGAAGTCAC TTCAGAAGGA GACCCTTCAT 
TTAGAATTTA CGGATCTGGA GTCCAAGACA GAGTTCGTGG GAGGATCCAC CCCAGAACAA 
ACTAAACTGG AGCTGACTTT TCGAGAGCTA TCTGGGTCGT TCCAGGAGGA GAAGGGAGGG 
CCATCTGTCA AGTTCTTCCA TGTGTCTGGT GGAGTAGATG GAGACACCGC ATCGTCAGAT 
GACTTTGACT GGCCACGAAT GGTACTGAAA ATAAACCCAC CAGCCATGCA TTCCATTTTG 
GAGAGAATAG CAGCCGAGGA AGAGGAAGAG AATGATGGTC ACTACCAGGA GGAGGAGGAC 
GGAGGGGCTC ATTCCCTGAA AGATGTCTGT GACCTGAGGC GGCCAGCCCC GTCTCCCTTT 
TCCTCTCGTC GAGTGATGTT TGAGAATGAG CAGATGGTGA TGCCGGGAGA CCCTGTGGAG 
ATGACGGAGT TTCAGGATAA AGCCATCAGC AACTCGCACT ACGTGCTGGA GCTCCTGTTG 
CCCAACATCC ACCTGACGCT GCCCAACAAA GGCTTCTACG AGAAGCTCTA TAACAGGATC 
TTTAACGACT TGCTTCTCTG GGAGCCAACA GCGCCTTCAC CAGTGGAGAC ACTTGAAAAC 
GTTTCCTATG GCATCGGACT CTCCGTGGCC AGTCAGCTGA TCAATACCTT CAGCAAAGAT 
AGCTTCAGTC CCTTTAAATC TGCAGTTCAC TATGATGAAG ACAGTGGATC TGAAGAAGAG 
ACTTTGCAGT ATTTTTCTGC TGTTGATCCC AACTATCGTT CCCGTAGGAA AAAAAAGTTG 
GACTCTCAGA ACAAGAACTC TCAGAGCTTC CTCTCAGTGC TTCTGAGTAT CAATCATGGA 
CTAATGGCAG TGTTCACTGA TGTGAAGCAA GAGAATGGAG ATCCGATGGA AAGTAAGCAT 
GGTGAATTCT GGCTAGAATT CAATAGCGGC TCGTTCTTCT GCGTGACAAA GTATGAGGGC 
TTTGAGGACA AGCACTACAT CTGCTTGCAT TCCAGCAGCC TCAGGCTGTA CCACAAAGGC 
ATAGTAGATG GAGCCATTCT CCCTACAGAG ACGCGCTTGC CCTGTTCCAC CCGCCCACAC 
TGGCTGGAAC CTACAATTTA TTCCTCTGAA GAAGATGGCC TCAGCCGCAC TGCCTCAGAT 
GGGGTTGGGG GAGACAATTT GAATATGCTC TCAGTCGCAG TGAAGATACT GTCTGATAAG 
TCCGAGTCCA ACACGAAGGA GTTCCTCGTG GCTGTGGGGC TGAAAGGAGC CACTCTACAG 
CACAGGGTGC TGCCCGCTGG ACTCAGTTGG CACGAGCAGA TTTTAAACTT CTTGAATATT 
GCTGATGAAC CTGTTTTGGG ATATAACCCT CCAACTTCAT TTACAACTTT CCATGTCCAT 
CTTTGGAGCT GTGCACTTGA TTACAGGCCC CTTCACCTGC CACTCCGATC CCTTCTTACT 
GTTGAGACGT TCAGCATTTC TAGCAGTGTT GCTTTGGATA AATCTTCCTC CACTCTCAGA 
ATCATCATGG ACGAGGCTGC TTTGCACCTG TCAGACAAGT GCAACACGGT CACTGTAAAC 
CTGAATAGAG ATTATGTTCG TGTGATGGAC ATGGGACTTT TAGAGCTGAC CATAACCGCA 
GTAAAATCTG ATTCTGATGG AGAACAGACT GCGCCCCGCT TTGAGTTACA CTGTTCCAGT 
GACGTCGTGC ACATCAGAAC ATGCTCAGAC TCATGTGCTG CGCTGATGAA CCTCATTCAG 
TATGTCGCAA GTTACGGGGA CCTACATGGA CCTCACAAAG CAGAGATGAA GCCTGGAGTC 
CCACAGAGAA AGCCCAAGGT AGATTCCAGC GCTCGGTCAT CTTCACATGG TCCAGTGCTT 
CCTGAAGCAG ATCAGCAGAT TTTACGGGAC CTGATGAGTG ATGCCATGGA GGAGATTGAT 
CTGCAGCAGG CCTCTGCCCC TGTCGGACCA CAGGCTAATG GTGTCCTGGA TGAAAAGTCT 
CACACTCAGG AGCCGTGCTG TTCAGACCTC TTCCTGTTCC CAGATGAGAG TGGAAATGTG 
TCCCAGGAGT CCAGCCCTGC CTATCCGTCT CTCACTCACC ACTTGATCAG TGACGCGGTG 
ACCGGTGTGA CCGCTGAAAA TGATGACTTC TGTATTCTTT TTGCACCAAA AACAGTCGTT 
CAGGAGAAGG AAGAAGAGCC AGTGATAAAG ATCATGGTTG ACGACGCCAT TGTGATAAAA 
GATGACTATT TCAGTCTGCC CATCACGAGG ACAGACAGCA GCAAAGCGCC GCTCCACTTT 
CCCATCCCTG CCGTCCGATA TGTTGTTAAA GAGGTCTCTC TGGTGTGGCA TCTTTATGGA 
GGCAAGGACT TTGCAACAGC TCCTCCAACT TCTCCAGCTA AGAGTTACAT TCCCCACAGC 
TCACCGTCTC AAACACCTAC AAGGCACGGG CGCCACACAG TCTGTGGGGG CAAAGGAAGG 
AACCATGACT TCTTGATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCTCCGTGTA AGCCTGAGTG TGAGTCCAGC CTCTTAGAAC ACCCAGTCTC CAGGCAGGTC 
TTCATCGTGC AGGACCTTGA AATTCGAGAT CGGCTGGCAA CATCACAGAT GAATAAGTTT 
TTATACCTGT ATTGCAGCAA AGACATGCCT CGTAAAGCCC ATTCCAACAT GCTGACAATT 
AAAGCGTTGC ATGTACGTCC GGAGTCTGGC AGGTCACCAC AGGAGTGCTG CTTGAGAGTG 
TCCCTGATGC CTCTTCGCCT CAATATTGAC CAGGACGCCT TGTTCTTCCT CAAGGATTTC 
TTCACAAGTC TTTCTACAGA AGTGGAGCTT CTACTGACTC CAGATCCAGA AGTTACAAAG 
TCTCCTGGAG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TGAGTACCTC AAAGGAGCCA 
AATCTAGTCG TTTCTTTTCC TGGGCCAAAA CAAGCTTCCC CGAACCATCG TGCCAATTCA 
GCGGAGGGTG GTAATGGACT GGAGGAGGAC GTCTCTGCTG AGGAAACTTC ATTTAGTGAC 
CAGCCTGTGT TTTTTAGAGA ATTTAGATTC ACGGCAGAAG TTCCCATCCG GCTTGACTAC 
CACGGCAAGC ACGTGTCGAT GGATCAGGGT ACATTGGCTG GGATTTTGAT TGGCCTGGCC 
CAGTTGAACT GTTCGGAGCT AAAGCTGAAA AGGCTTTTCT ATCGGCATGG TTTGCTAGGT 
ATTGACAAAT TGTTCTCATA TGCAATCTCG GAATGGCTCA GTGACATTAA GAAGAACCAG 
CTACCAGGAA TCCTGGGAGG TGTTGGCCCT ATGCATTCAC TAGTTCAATT AGTGCAAGGC 
CTGAAGGACT TGGTATGGCT GCCCATTGAG CAATACCGGA AGGATGGCCG TATCGTCAGA 
GGCTTCCAGA GGGGCGCTGC TTCCTTTGGT ACCTCAACTG CAATGGCTGC CCTGGAACTC 
ACAAACAGAA TGGTCCAAAC CATACAGGCA GCCGCAGAGA CTGCATATGA CATGGTGTCT 
CCGAGTACCC TTTCCATTGA GCCCAAGAAG GCAAAGAGAT TTCCTCATCA CCGGTTAGCC 
CACCAGCCAG TAGACCTGAG GGAAGGCGTG GCCAAGGCCT ACAGCGTTGT AAAAGAGGGG 
ATCACAGACA CAGCTCAGAC CATCTATGAA ACGGCGGCTC GGGAACACGA GAGCAGAGGG 
GTCACTGGTG CTGTGGGTGA AGTCCTGCGG CAGATCCCAC CGGCAGTGGT CAGACCCCTG 
ATTGTAGCCA CCGAGGCGAC ATCAAATGTG TTAGGTGGCA TGAGAAACCA AATTAGACCA 
GACGTTCGGC AGGACGAGTC GCAGAAATGG CGCCATGGGG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083930.5
CDS	412..6639
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRVATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEASQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGAALEIRVDRTVYCDETADESSGVNVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRTLPEIAPSDPVQIGRLLGRLELSLTLKQNEVLPGAKLDVDGQIDSFHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSMGVSSEKSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPHMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFADHKEQPVRGPAFQSDVVHPASLQKAALSSRSASVDESRPEFICRLALGIFSVSVLHIDPLSPAETSLNVNPLTRMATDFFSCVEKMDPAIFSTGDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRHFSTDMSVGQMEFLECLFPTDCHSVPSHYTELLTFHSKEGTDAHLPVCLQLHYKHSETRGPQGNQARLSSVPQKAELQIKLNPVCCELDISIVDRLNSLLQPQKLTTVEMMASHMYASYNKHISLHKAFTEVFLDDSHSPANRRVSVQVATPALHLSVRFPIPDLRSDQERGPWFKKSLQKETLHLEFTDLESKTEFVGGSTPEQTKLELTFRELSGSFQEEKGGPSVKFFHVSGGVDGDTASSDDFDWPRMVLKINPPAMHSILERIAAEEEEENDGHYQEEEDGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELLLPNIHLTLPNKGFYEKLYNRIFNDLLLWEPTAPSPVETLENVSYGIGLSVASQLINTFSKDSFSPFKSAVHYDEDSGSEEETLQYFSAVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVKQENGDPMESKHGEFWLEFNSGSFFCVTKYEGFEDKHYICLHSSSLRLYHKGIVDGAILPTETRLPCSTRPHWLEPTIYSSEEDGLSRTASDGVGGDNLNMLSVAVKILSDKSESNTKEFLVAVGLKGATLQHRVLPAGLSWHEQILNFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLHLPLRSLLTVETFSISSSVALDKSSSTLRIIMDEAALHLSDKCNTVTVNLNRDYVRVMDMGLLELTITAVKSDSDGEQTAPRFELHCSSDVVHIRTCSDSCAALMNLIQYVASYGDLHGPHKAEMKPGVPQRKPKVDSSARSSSHGPVLPEADQQILRDLMSDAMEEIDLQQASAPVGPQANGVLDEKSHTQEPCCSDLFLFPDESGNVSQESSPAYPSLTHHLISDAVTGVTAENDDFCILFAPKTVVQEKEEEPVIKIMVDDAIVIKDDYFSLPITRTDSSKAPLHFPIPAVRYVVKEVSLVWHLYGGKDFATAPPTSPAKSYIPHSSPSQTPTRHGRHTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLLEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKDMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLLTPDPEVTKSPGADVTCSLPRHLSTSKEPNLVVSFPGPKQASPNHRANSAEGGNGLEEDVSAEETSFSDQPVFFREFRFTAEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGIDKLFSYAISEWLSDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPSTLSIEPKKAKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVRPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	NM_029654.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy related 2B (Atg2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029654.4

ORF Insert Sequence:

ATGCCTTGGC CATTTTCAGA ATCCATCAAG AAGAGGGCCT GTCGCTACCT CCTACAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTGAGCCT GGATCTGTAC 
CAGGGCACCG GGTCCCTTGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAAATCCTG 
GAGTCAGCAG ATGCACCATT AGAAGTCACT GAAGGATTTA TTCAGTCCAT TTCCCTGTCC 
GTCCCATGGG GCTCCTTGCT GCAGGACAAC TGTGCCCTGG AAGTGAGAGG ACTGGAGATG 
GTCTTCCGGC CTCGACCTCG AGTAGCAACT GGGTCCGAGC CTATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAGCT GGCAAAGGAG TGCCTTAGCC AGAAACTGAC AGATGAGCAA 
GGAGAGGCAT CCCAACCTTT CGAGGGACTG GAAAAGTTTG CAGAAACCAT CGAAACAGTA 
CTAAGAAGAG TGAAAGTCAC TTTTATAGAT ACTGTTTTAA GAATTGAACA TGTGCCAGAA 
AATTCTAAAA CTGGAGCTGC ACTTGAAATC CGAGTAGACA GGACCGTGTA CTGTGACGAA 
ACAGCCGACG AGTCCTCAGG AGTCAATGTG CATCAGCCCA CAGCTTTTGC TCATAAATTG 
CTGCAGCTCT CTGGGGTGTC TCTGTTCTGG GATGAGTTTT CTGCTTCAGC AAAATCTTCC 
CCCGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAACCCC 
AAAATTGTTT ATGAGCCACA CCCACAACTA ACTAGAACGT TACCGGAGAT CGCACCCTCG 
GATCCAGTGC AGATTGGAAG GCTACTGGGC AGGCTGGAGT TGAGTCTCAC TTTAAAACAG 
AATGAAGTGC TTCCAGGAGC CAAGTTGGAT GTCGATGGAC AGATAGACTC CTTTCATCTA 
TTCCTGTCAC CGAGGCAGGT ACACCTCCTT CTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GCTGGCTAAT AAAGACAGGA AAAATCGACC CATGCAGCAG 
GAAGATGAGT ACAGAATTCA GATGGAATTA AACCGGTATT ATCTGAGGAA AGATTCCCTC 
TCTATGGGTG TGTCTTCTGA GAAGAGCTTC TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAGG AAGAAGTTTT CTTCTCCATG GCCGACATGG ACATGTCTCA CAGTCTGTCC 
TCTCTTCCAC CCCTTGGGGA CCCACCACAC ATGGACCTGG AGCTGTCGCT GACGAGTACA 
TACACCAACA CACCCGCAGG GTCCCCGCTA AGCGCCACTG TGCTTCAGCC AACTTGGGGA 
GAGTTTGCCG ACCACAAAGA ACAGCCAGTC AGAGGGCCAG CATTCCAGTC TGACGTGGTG 
CACCCGGCAT CTTTACAAAA AGCTGCTCTC TCCTCTAGGT CTGCTTCAGT GGATGAATCC 
AGACCTGAGT TTATCTGCAG GCTGGCTCTC GGCATATTCT CCGTCTCCGT GCTTCACATC 
GATCCTTTGT CTCCAGCGGA AACGTCTCTG AACGTCAACC CATTGACACG CATGGCCACC 
GATTTCTTCT CCTGTGTAGA AAAGATGGAC CCAGCAATAT TTTCGACGGG AGACTTTAAG 
TCTTTCCGAG CAGTCTTTGC CGAAGCTTGT TCACACGATC ACCTTAGGTT TATAGGTACT 
GGCATCAAAG TGTCCTATGA GCAGAGACAA AGGTCAGCTT CCCGGCACTT TAGCACCGAC 
ATGTCCGTTG GGCAAATGGA GTTTTTGGAG TGTCTGTTTC CAACAGATTG TCATTCTGTC 
CCTTCTCACT ACACCGAGCT TCTAACGTTC CATTCCAAAG AAGGGACGGA TGCCCACCTT 
CCCGTTTGTC TTCAGCTTCA CTATAAACAC TCAGAGACTA GAGGACCCCA GGGTAATCAA 
GCAAGACTTA GTTCAGTTCC TCAAAAGGCA GAATTACAAA TTAAATTAAA CCCCGTGTGT 
TGCGAGCTGG ATATCAGTAT TGTGGATAGG TTAAATTCCT TGCTTCAACC TCAGAAACTC 
ACTACCGTGG AGATGATGGC CTCGCACATG TATGCGTCCT ATAACAAGCA TATTAGTTTG 
CACAAGGCTT TCACGGAAGT GTTCCTAGAT GACTCACACA GTCCTGCAAA CCGTCGGGTG 
TCCGTACAAG TTGCCACCCC AGCCCTGCAC CTGTCTGTTC GCTTCCCTAT TCCTGATCTA 
AGATCAGACC AGGAAAGAGG ACCCTGGTTT AAGAAGTCAC TTCAGAAGGA GACCCTTCAT 
TTAGAATTTA CGGATCTGGA GTCCAAGACA GAGTTCGTGG GAGGATCCAC CCCAGAACAA 
ACTAAACTGG AGCTGACTTT TCGAGAGCTA TCTGGGTCGT TCCAGGAGGA GAAGGGAGGG 
CCATCTGTCA AGTTCTTCCA TGTGTCTGGT GGAGTAGATG GAGACACCGC ATCGTCAGAT 
GACTTTGACT GGCCACGAAT GGTACTGAAA ATAAACCCAC CAGCCATGCA TTCCATTTTG 
GAGAGAATAG CAGCCGAGGA AGAGGAAGAG AATGATGGTC ACTACCAGGA GGAGGAGGAC 
GGAGGGGCTC ATTCCCTGAA AGATGTCTGT GACCTGAGGC GGCCAGCCCC GTCTCCCTTT 
TCCTCTCGTC GAGTGATGTT TGAGAATGAG CAGATGGTGA TGCCGGGAGA CCCTGTGGAG 
ATGACGGAGT TTCAGGATAA AGCCATCAGC AACTCGCACT ACGTGCTGGA GCTCCTGTTG 
CCCAACATCC ACCTGACGCT GCCCAACAAA GGCTTCTACG AGAAGCTCTA TAACAGGATC 
TTTAACGACT TGCTTCTCTG GGAGCCAACA GCGCCTTCAC CAGTGGAGAC ACTTGAAAAC 
GTTTCCTATG GCATCGGACT CTCCGTGGCC AGTCAGCTGA TCAATACCTT CAGCAAAGAT 
AGCTTCAGTC CCTTTAAATC TGCAGTTCAC TATGATGAAG ACAGTGGATC TGAAGAAGAG 
ACTTTGCAGT ATTTTTCTGC TGTTGATCCC AACTATCGTT CCCGTAGGAA AAAAAAGTTG 
GACTCTCAGA ACAAGAACTC TCAGAGCTTC CTCTCAGTGC TTCTGAGTAT CAATCATGGA 
CTAATGGCAG TGTTCACTGA TGTGAAGCAA GAGAATGGAG ATCCGATGGA AAGTAAGCAT 
GGTGAATTCT GGCTAGAATT CAATAGCGGC TCGTTCTTCT GCGTGACAAA GTATGAGGGC 
TTTGAGGACA AGCACTACAT CTGCTTGCAT TCCAGCAGCC TCAGGCTGTA CCACAAAGGC 
ATAGTAGATG GAGCCATTCT CCCTACAGAG ACGCGCTTGC CCTGTTCCAC CCGCCCACAC 
TGGCTGGAAC CTACAATTTA TTCCTCTGAA GAAGATGGCC TCAGCCGCAC TGCCTCAGAT 
GGGGTTGGGG GAGACAATTT GAATATGCTC TCAGTCGCAG TGAAGATACT GTCTGATAAG 
TCCGAGTCCA ACACGAAGGA GTTCCTCGTG GCTGTGGGGC TGAAAGGAGC CACTCTACAG 
CACAGGGTGC TGCCCGCTGG ACTCAGTTGG CACGAGCAGA TTTTAAACTT CTTGAATATT 
GCTGATGAAC CTGTTTTGGG ATATAACCCT CCAACTTCAT TTACAACTTT CCATGTCCAT 
CTTTGGAGCT GTGCACTTGA TTACAGGCCC CTTCACCTGC CACTCCGATC CCTTCTTACT 
GTTGAGACGT TCAGCATTTC TAGCAGTGTT GCTTTGGATA AATCTTCCTC CACTCTCAGA 
ATCATCATGG ACGAGGCTGC TTTGCACCTG TCAGACAAGT GCAACACGGT CACTGTAAAC 
CTGAATAGAG ATTATGTTCG TGTGATGGAC ATGGGACTTT TAGAGCTGAC CATAACCGCA 
GTAAAATCTG ATTCTGATGG AGAACAGACT GCGCCCCGCT TTGAGTTACA CTGTTCCAGT 
GACGTCGTGC ACATCAGAAC ATGCTCAGAC TCATGTGCTG CGCTGATGAA CCTCATTCAG 
TATGTCGCAA GTTACGGGGA CCTACATGGA CCTCACAAAG CAGAGATGAA GCCTGGAGTC 
CCACAGAGAA AGCCCAAGGT AGATTCCAGC GCTCGGTCAT CTTCACATGG TCCAGTGCTT 
CCTGAAGCAG ATCAGCAGAT TTTACGGGAC CTGATGAGTG ATGCCATGGA GGAGATTGAT 
CTGCAGCAGG CCTCTGCCCC TGTCGGACCA CAGGCTAATG GTGTCCTGGA TGAAAAGTCT 
CACACTCAGG AGCCGTGCTG TTCAGACCTC TTCCTGTTCC CAGATGAGAG TGGAAATGTG 
TCCCAGGAGT CCAGCCCTGC CTATCCGTCT CTCACTCACC ACTTGATCAG TGACGCGGTG 
ACCGGTGTGA CCGCTGAAAA TGATGACTTC TGTATTCTTT TTGCACCAAA AACAGTCGTT 
CAGGAGAAGG AAGAAGAGCC AGTGATAAAG ATCATGGTTG ACGACGCCAT TGTGATAAAA 
GATGACTATT TCAGTCTGCC CATCACGAGG ACAGACAGCA GCAAAGCGCC GCTCCACTTT 
CCCATCCCTG CCGTCCGATA TGTTGTTAAA GAGGTCTCTC TGGTGTGGCA TCTTTATGGA 
GGCAAGGACT TTGCAACAGC TCCTCCAACT TCTCCAGCTA AGAGTTACAT TCCCCACAGC 
TCACCGTCTC AAACACCTAC AAGGCACGGG CGCCACACAG TCTGTGGGGG CAAAGGAAGG 
AACCATGACT TCTTGATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCTCCGTGTA AGCCTGAGTG TGAGTCCAGC CTCTTAGAAC ACCCAGTCTC CAGGCAGGTC 
TTCATCGTGC AGGACCTTGA AATTCGAGAT CGGCTGGCAA CATCACAGAT GAATAAGTTT 
TTATACCTGT ATTGCAGCAA AGACATGCCT CGTAAAGCCC ATTCCAACAT GCTGACAATT 
AAAGCGTTGC ATGTACGTCC GGAGTCTGGC AGGTCACCAC AGGAGTGCTG CTTGAGAGTG 
TCCCTGATGC CTCTTCGCCT CAATATTGAC CAGGACGCCT TGTTCTTCCT CAAGGATTTC 
TTCACAAGTC TTTCTACAGA AGTGGAGCTT CTACTGACTC CAGATCCAGA AGTTACAAAG 
TCTCCTGGAG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TGAGTACCTC AAAGGAGCCA 
AATCTAGTCG TTTCTTTTCC TGGGCCAAAA CAAGCTTCCC CGAACCATCG TGCCAATTCA 
GCGGAGGGTG GTAATGGACT GGAGGAGGAC GTCTCTGCTG AGGAAACTTC ATTTAGTGAC 
CAGCCTGTGT TTTTTAGAGA ATTTAGATTC ACGGCAGAAG TTCCCATCCG GCTTGACTAC 
CACGGCAAGC ACGTGTCGAT GGATCAGGGT ACATTGGCTG GGATTTTGAT TGGCCTGGCC 
CAGTTGAACT GTTCGGAGCT AAAGCTGAAA AGGCTTTTCT ATCGGCATGG TTTGCTAGGT 
ATTGACAAAT TGTTCTCATA TGCAATCTCG GAATGGCTCA GTGACATTAA GAAGAACCAG 
CTACCAGGAA TCCTGGGAGG TGTTGGCCCT ATGCATTCAC TAGTTCAATT AGTGCAAGGC 
CTGAAGGACT TGGTATGGCT GCCCATTGAG CAATACCGGA AGGATGGCCG TATCGTCAGA 
GGCTTCCAGA GGGGCGCTGC TTCCTTTGGT ACCTCAACTG CAATGGCTGC CCTGGAACTC 
ACAAACAGAA TGGTCCAAAC CATACAGGCA GCCGCAGAGA CTGCATATGA CATGGTGTCT 
CCGAGTACCC TTTCCATTGA GCCCAAGAAG GCAAAGAGAT TTCCTCATCA CCGGTTAGCC 
CACCAGCCAG TAGACCTGAG GGAAGGCGTG GCCAAGGCCT ACAGCGTTGT AAAAGAGGGG 
ATCACAGACA CAGCTCAGAC CATCTATGAA ACGGCGGCTC GGGAACACGA GAGCAGAGGG 
GTCACTGGTG CTGTGGGTGA AGTCCTGCGG CAGATCCCAC CGGCAGTGGT CAGACCCCTG 
ATTGTAGCCA CCGAGGCGAC ATCAAATGTG TTAGGTGGCA TGAGAAACCA AATTAGACCA 
GACGTTCGGC AGGACGAGTC GCAGAAATGG CGCCATGGGG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg2b ( XM_006516360.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516423.1 Mus musculus autophagy related 2B (Atg2b), transcript variant X1, mRNA.

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CloneID	OMu48184
Clone ID Related Accession (Same CDS sequence)	XM_006516360.1 , XM_006516360.2
Accession Version	XM_006516360.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CATTTTCAGA ATCCATCAAG AAGAGGGCCT GTCGCTACCT CCTACAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTGAGCCT GGATCTGTAC 
CAGGGCACCG GGTCCCTTGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAAATCCTG 
GAGTCAGCAG ATGCACCATT AGAAGTCACT GAAGGATTTA TTCAGTCCAT TTCCCTGTCC 
GTCCCATGGG GCTCCTTGCT GCAGGACAAC TGTGCCCTGG AAGTGAGAGG ACTGGAGATG 
GTCTTCCGGC CTCGACCTCG AGTAGCAACT GGGTCCGAGC CTATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAGCT GGCAAAGGAG TGCCTTAGCC AGAAACTGAC AGATGAGCAA 
GGAGAGGCAT CCCAACCTTT CGAGGGACTG GAAAAGTTTG CAGAAACCAT CGAAACAGTA 
CTAAGAAGAG TGAAAGTCAC TTTTATAGAT ACTGTTTTAA GAATTGAACA TGTGCCAGAA 
AATTCTAAAA CTGGAGCTGC ACTTGAAATC CGAGTAGACA GGACCGTGTA CTGTGACGAA 
ACAGCCGACG AGTCCTCAGG AGTCAATGTG CATCAGCCCA CAGCTTTTGC TCATAAATTG 
CTGCAGCTCT CTGGGGTGTC TCTGTTCTGG GATGAGTTTT CTGCTTCAGC AAAATCTTCC 
CCCGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAACCCC 
AAAATTGTTT ATGAGCCACA CCCACAACTA ACTAGAACGT TACCGGAGAT CGCACCCTCG 
GATCCAGTGC AGATTGGAAG GCTACTGGGC AGGCTGGAGT TGAGTCTCAC TTTAAAACAG 
AATGAAGTGC TTCCAGGAGC CAAGTTGGAT GTCGATGGAC AGATAGACTC CTTTCATCTA 
TTCCTGTCAC CGAGGCAGGT ACACCTCCTT CTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GCTGGCTAAT AAAGACAGGA AAAATCGACC CATGCAGCAG 
GAAGATGAGT ACAGAATTCA GATGGAATTA AACCGGTATT ATCTGAGGAA AGATTCCCTC 
TCTATGGGTG TGTCTTCTGA GAAGAGCTTC TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAGG AAGAAGTTTT CTTCTCCATG GCCGACATGG ACATGTCTCA CAGTCTGTCC 
TCTCTTCCAC CCCTTGGGGA CCCACCACAC ATGGACCTGG AGCTGTCGCT GACGAGTACA 
TACACCAACA CACCCGCAGG GTCCCCGCTA AGCGCCACTG TGCTTCAGCC AACTTGGGGA 
GAGTTTGCCG ACCACAAAGA ACAGCCAGTC AGAGGGCCAG CATTCCAGTC TGACGTGGTG 
CACCCGGCAT CTTTACAAAA AGCTGCTCTC TCCTCTAGGT CTGCTTCAGT GGATGAATCC 
AGACCTGAGT TTATCTGCAG GCTGGCTCTC GGCATATTCT CCGTCTCCGT GCTTCACATC 
GATCCTTTGT CTCCAGCGGA AACGTCTCTG AACGTCAACC CATTGACACG CATGGCCACC 
GATTTCTTCT CCTGTGTAGA AAAGATGGAC CCAGCAATAT TTTCGACGGG AGACTTTAAG 
TCTTTCCGAG CAGTCTTTGC CGAAGCTTGT TCACACGATC ACCTTAGGTT TATAGGTACT 
GGCATCAAAG TGTCCTATGA GCAGAGACAA AGGTCAGCTT CCCGGCACTT TAGCACCGAC 
ATGTCCGTTG GGCAAATGGA GTTTTTGGAG TGTCTGTTTC CAACAGATTG TCATTCTGTC 
CCTTCTCACT ACACCGAGCT TCTAACGTTC CATTCCAAAG AAGGGACGGA TGCCCACCTT 
CCCGTTTGTC TTCAGCTTCA CTATAAACAC TCAGAGACTA GAGGACCCCA GGGTAATCAA 
GCAAGACTTA GTTCAGTTCC TCAAAAGGCA GAATTACAAA TTAAATTAAA CCCCGTGTGT 
TGCGAGCTGG ATATCAGTAT TGTGGATAGG TTAAATTCCT TGCTTCAACC TCAGAAACTC 
ACTACCGTGG AGATGATGGC CTCGCACATG TATGCGTCCT ATAACAAGCA TATTAGTTTG 
CACAAGGCTT TCACGGAAGT GTTCCTAGAT GACTCACACA GTCCTGCAAA CCGTCGGGTG 
TCCGTACAAG TTGCCACCCC AGCCCTGCAC CTGTCTGTTC GCTTCCCTAT TCCTGATCTA 
AGATCAGACC AGGAAAGAGG ACCCTGGTTT AAGAAGTCAC TTCAGAAGGA GACCCTTCAT 
TTAGAATTTA CGGATCTGGA GTCCAAGACA GAGTTCGTGG GAGGATCCAC CCCAGAACAA 
ACTAAACTGG AGCTGACTTT TCGAGAGCTA TCTGGGTCGT TCCAGGAGGA GAAGGGAGGG 
CCATCTGTCA AGTTCTTCCA TGTGTCTGGT GGAGTAGATG GAGACACCGC ATCGTCAGAT 
GACTTTGACT GGCCACGAAT GGTACTGAAA ATAAACCCAC CAGCCATGCA TTCCATTTTG 
GAGAGAATAG CAGCCGAGGA AGAGGAAGAG AATGATGGTC ACTACCAGGA GGAGGAGGAC 
GGAGGGGCTC ATTCCCTGAA AGATGTCTGT GACCTGAGGC GGCCAGCCCC GTCTCCCTTT 
TCCTCTCGTC GAGTGATGTT TGAGAATGAG CAGATGGTGA TGCCGGGAGA CCCTGTGGAG 
ATGACGGAGT TTCAGGATAA AGCCATCAGC AACTCGCACT ACGTGCTGGA GCTCCTGTTG 
CCCAACATCC ACCTGACGCT GCCCAACAAA GGCTTCTACG AGAAGCTCTA TAACAGGATC 
TTTAACGACT TGCTTCTCTG GGAGCCAACA GCGCCTTCAC CAGTGGAGAC ACTTGAAAAC 
GTTTCCTATG GCATCGGACT CTCCGTGGCC AGTCAGCTGA TCAATACCTT CAGCAAAGAT 
AGCTTCAGTC CCTTTAAATC TGCAGTTCAC TATGATGAAG ACAGTGGATC TGAAGAAGAG 
ACTTTGCAGT ATTTTTCTGC TGTTGATCCC AACTATCGTT CCCGTAGGAA AAAAAAGTTG 
GACTCTCAGA ACAAGAACTC TCAGAGCTTC CTCTCAGTGC TTCTGAGTAT CAATCATGGA 
CTAATGGCAG TGTTCACTGA TGTGAAGCAA GAGAATGGAG ATCCGATGGA AAGTAAGCAT 
GGTGAATTCT GGCTAGAATT CAATAGCGGC TCGTTCTTCT GCGTGACAAA GTATGAGGGC 
TTTGAGGACA AGCACTACAT CTGCTTGCAT TCCAGCAGCC TCAGGCTGTA CCACAAAGGC 
ATAGTAGATG GAGCCATTCT CCCTACAGAG ACGCGCTTGC CCTGTTCCAC CCGCCCACAC 
TGGCTGGAAC CTACAATTTA TTCCTCTGAA GAAGATGGCC TCAGCCGCAC TGCCTCAGAT 
GGGGTTGGGG GAGACAATTT GAATATGCTC TCAGTCGCAG TGAAGATACT GTCTGATAAG 
TCCGAGTCCA ACACGAAGGA GTTCCTCGTG GCTGTGGGGC TGAAAGGAGC CACTCTACAG 
CACAGGGTGC TGCCCGCTGG ACTCAGTTGG CACGAGCAGA TTTTAAACTT CTTGAATATT 
GCTGATGAAC CTGTTTTGGG ATATAACCCT CCAACTTCAT TTACAACTTT CCATGTCCAT 
CTTTGGAGCT GTGCACTTGA TTACAGGCCC CTTCACCTGC CACTCCGATC CCTTCTTACT 
GTTGAGACGT TCAGCATTTC TAGCAGTGTT GCTTTGGATA AATCTTCCTC CACTCTCAGA 
ATCATCATGG ACGAGGCTGC TTTGCACCTG TCAGACAAGT GCAACACGGT CACTGTAAAC 
CTGAATAGAG ATTATGTTCG TGTGATGGAC ATGGGACTTT TAGAGCTGAC CATAACCGCA 
GTAAAATCTG ATTCTGATGG AGAACAGACT GCGCCCCGCT TTGAGTTACA CTGTTCCAGT 
GACGTCGTGC ACATCAGAAC ATGCTCAGAC TCATGTGCTG CGCTGATGAA CCTCATTCAG 
TATGTCGCAA GTTACGGGGA CCTACATGGA CCTCACAAAG CAGAGATGAA GCCTGGAGTC 
CCACAGAGAA AGCCCAAGGT AGATTCCAGC GCTCGGTCAT CTTCACATGG TCCAGTGCTT 
CCTGAAGCAG ATCAGCAGAT TTTACGGGAC CTGATGAGTG ATGCCATGGA GGAGATTGAT 
CTGCAGCAGG CCTCTGCCCC TGTCGGACCA CAGGCTAATG GTGTCCTGGA TGAAAAGTCT 
CACACTCAGG AGCCGTGCTG TTCAGACCTC TTCCTGTTCC CAGATGAGAG TGGAAATGTG 
TCCCAGGAGT CCAGCCCTGC CTATCCGTCT CTCACTCACC ACTTGATCAG TGACGCGGTG 
ACCGGTGTGA CCGCTGAAAA TGATGACTTC TGTATTCTTT TTGCACCAAA AACAGTCGTT 
CAGGAGAAGG AAGAAGAGCC AGTGATAAAG ATCATGGTTG ACGACGCCAT TGTGATAAAA 
GATGACTATT TCAGTCTGCC CATCACGAGG ACAGACAGCA GCAAAGCGCC GCTCCACTTT 
CCCATCCCTG CCGTCCGATA TGTTGTTAAA GAGGTCTCTC TGGTGTGGCA TCTTTATGGA 
GGCAAGGACT TTGCAACAGC TCCTCCAACT TCTCCAGCTA AGAGTTACAT TAGTCCCCAC 
AGCTCACCGT CTCAAACACC TACAAGGCAC GGGCGCCACA CAGTCTGTGG GGGCAAAGGA 
AGGAACCATG ACTTCTTGAT GGAAATACAG TTAAGCAAGG TGAAGTTTCA GCATGAGGTC 
TACCCTCCGT GTAAGCCTGA GTGTGAGTCC AGCCTCTTAG AACACCCAGT CTCCAGGCAG 
GTCTTCATCG TGCAGGACCT TGAAATTCGA GATCGGCTGG CAACATCACA GATGAATAAG 
TTTTTATACC TGTATTGCAG CAAAGACATG CCTCGTAAAG CCCATTCCAA CATGCTGACA 
ATTAAAGCGT TGCATGTACG TCCGGAGTCT GGCAGGTCAC CACAGGAGTG CTGCTTGAGA 
GTGTCCCTGA TGCCTCTTCG CCTCAATATT GACCAGGACG CCTTGTTCTT CCTCAAGGAT 
TTCTTCACAA GTCTTTCTAC AGAAGTGGAG CTTCTACTGA CTCCAGATCC AGAAGTTACA 
AAGTCTCCTG GAGCTGATGT CACCTGCAGT TTGCCAAGGC ATTTGAGTAC CTCAAAGGAG 
CCAAATCTAG TCGTTTCTTT TCCTGGGCCA AAACAAGCTT CCCCGAACCA TCGTGCCAAT 
TCAGCGGAGG GTGGTAATGG ACTGGAGGAG GACGTCTCTG CTGAGGAAAC TTCATTTAGT 
GACCAGCCTG TGTTTTTTAG AGAATTTAGA TTCACGGCAG AAGTTCCCAT CCGGCTTGAC 
TACCACGGCA AGCACGTGTC GATGGATCAG GGTACATTGG CTGGGATTTT GATTGGCCTG 
GCCCAGTTGA ACTGTTCGGA GCTAAAGCTG AAAAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTATTGACA AATTGTTCTC ATATGCAATC TCGGAATGGC TCAGTGACAT TAAGAAGAAC 
CAGCTACCAG GAATCCTGGG AGGTGTTGGC CCTATGCATT CACTAGTTCA ATTAGTGCAA 
GGCCTGAAGG ACTTGGTATG GCTGCCCATT GAGCAATACC GGAAGGATGG CCGTATCGTC 
AGAGGCTTCC AGAGGGGCGC TGCTTCCTTT GGTACCTCAA CTGCAATGGC TGCCCTGGAA 
CTCACAAACA GAATGGTCCA AACCATACAG GCAGCCGCAG AGACTGCATA TGACATGGTG 
TCTCCGAGTA CCCTTTCCAT TGAGCCCAAG AAGGCAAAGA GATTTCCTCA TCACCGGTTA 
GCCCACCAGC CAGTAGACCT GAGGGAAGGC GTGGCCAAGG CCTACAGCGT TGTAAAAGAG 
GGGATCACAG ACACAGCTCA GACCATCTAT GAAACGGCGG CTCGGGAACA CGAGAGCAGA 
GGGGTCACTG GTGCTGTGGG TGAAGTCCTG CGGCAGATCC CACCGGCAGT GGTCAGACCC 
CTGATTGTAG CCACCGAGGC GACATCAAAT GTGTTAGGTG GCATGAGAAA CCAAATTAGA 
CCAGACGTTC GGCAGGACGA GTCGCAGAAA TGGCGCCATG GGGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516423.1
CDS	412..6642
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRVATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEASQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGAALEIRVDRTVYCDETADESSGVNVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRTLPEIAPSDPVQIGRLLGRLELSLTLKQNEVLPGAKLDVDGQIDSFHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSMGVSSEKSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPHMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFADHKEQPVRGPAFQSDVVHPASLQKAALSSRSASVDESRPEFICRLALGIFSVSVLHIDPLSPAETSLNVNPLTRMATDFFSCVEKMDPAIFSTGDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRHFSTDMSVGQMEFLECLFPTDCHSVPSHYTELLTFHSKEGTDAHLPVCLQLHYKHSETRGPQGNQARLSSVPQKAELQIKLNPVCCELDISIVDRLNSLLQPQKLTTVEMMASHMYASYNKHISLHKAFTEVFLDDSHSPANRRVSVQVATPALHLSVRFPIPDLRSDQERGPWFKKSLQKETLHLEFTDLESKTEFVGGSTPEQTKLELTFRELSGSFQEEKGGPSVKFFHVSGGVDGDTASSDDFDWPRMVLKINPPAMHSILERIAAEEEEENDGHYQEEEDGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELLLPNIHLTLPNKGFYEKLYNRIFNDLLLWEPTAPSPVETLENVSYGIGLSVASQLINTFSKDSFSPFKSAVHYDEDSGSEEETLQYFSAVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVKQENGDPMESKHGEFWLEFNSGSFFCVTKYEGFEDKHYICLHSSSLRLYHKGIVDGAILPTETRLPCSTRPHWLEPTIYSSEEDGLSRTASDGVGGDNLNMLSVAVKILSDKSESNTKEFLVAVGLKGATLQHRVLPAGLSWHEQILNFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLHLPLRSLLTVETFSISSSVALDKSSSTLRIIMDEAALHLSDKCNTVTVNLNRDYVRVMDMGLLELTITAVKSDSDGEQTAPRFELHCSSDVVHIRTCSDSCAALMNLIQYVASYGDLHGPHKAEMKPGVPQRKPKVDSSARSSSHGPVLPEADQQILRDLMSDAMEEIDLQQASAPVGPQANGVLDEKSHTQEPCCSDLFLFPDESGNVSQESSPAYPSLTHHLISDAVTGVTAENDDFCILFAPKTVVQEKEEEPVIKIMVDDAIVIKDDYFSLPITRTDSSKAPLHFPIPAVRYVVKEVSLVWHLYGGKDFATAPPTSPAKSYISPHSSPSQTPTRHGRHTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLLEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKDMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLLTPDPEVTKSPGADVTCSLPRHLSTSKEPNLVVSFPGPKQASPNHRANSAEGGNGLEEDVSAEETSFSDQPVFFREFRFTAEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGIDKLFSYAISEWLSDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPSTLSIEPKKAKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVRPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_006516360.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy related 2B (Atg2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516360.1

ORF Insert Sequence:

ATGCCTTGGC CATTTTCAGA ATCCATCAAG AAGAGGGCCT GTCGCTACCT CCTACAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTGAGCCT GGATCTGTAC 
CAGGGCACCG GGTCCCTTGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAAATCCTG 
GAGTCAGCAG ATGCACCATT AGAAGTCACT GAAGGATTTA TTCAGTCCAT TTCCCTGTCC 
GTCCCATGGG GCTCCTTGCT GCAGGACAAC TGTGCCCTGG AAGTGAGAGG ACTGGAGATG 
GTCTTCCGGC CTCGACCTCG AGTAGCAACT GGGTCCGAGC CTATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAGCT GGCAAAGGAG TGCCTTAGCC AGAAACTGAC AGATGAGCAA 
GGAGAGGCAT CCCAACCTTT CGAGGGACTG GAAAAGTTTG CAGAAACCAT CGAAACAGTA 
CTAAGAAGAG TGAAAGTCAC TTTTATAGAT ACTGTTTTAA GAATTGAACA TGTGCCAGAA 
AATTCTAAAA CTGGAGCTGC ACTTGAAATC CGAGTAGACA GGACCGTGTA CTGTGACGAA 
ACAGCCGACG AGTCCTCAGG AGTCAATGTG CATCAGCCCA CAGCTTTTGC TCATAAATTG 
CTGCAGCTCT CTGGGGTGTC TCTGTTCTGG GATGAGTTTT CTGCTTCAGC AAAATCTTCC 
CCCGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAACCCC 
AAAATTGTTT ATGAGCCACA CCCACAACTA ACTAGAACGT TACCGGAGAT CGCACCCTCG 
GATCCAGTGC AGATTGGAAG GCTACTGGGC AGGCTGGAGT TGAGTCTCAC TTTAAAACAG 
AATGAAGTGC TTCCAGGAGC CAAGTTGGAT GTCGATGGAC AGATAGACTC CTTTCATCTA 
TTCCTGTCAC CGAGGCAGGT ACACCTCCTT CTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GCTGGCTAAT AAAGACAGGA AAAATCGACC CATGCAGCAG 
GAAGATGAGT ACAGAATTCA GATGGAATTA AACCGGTATT ATCTGAGGAA AGATTCCCTC 
TCTATGGGTG TGTCTTCTGA GAAGAGCTTC TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAGG AAGAAGTTTT CTTCTCCATG GCCGACATGG ACATGTCTCA CAGTCTGTCC 
TCTCTTCCAC CCCTTGGGGA CCCACCACAC ATGGACCTGG AGCTGTCGCT GACGAGTACA 
TACACCAACA CACCCGCAGG GTCCCCGCTA AGCGCCACTG TGCTTCAGCC AACTTGGGGA 
GAGTTTGCCG ACCACAAAGA ACAGCCAGTC AGAGGGCCAG CATTCCAGTC TGACGTGGTG 
CACCCGGCAT CTTTACAAAA AGCTGCTCTC TCCTCTAGGT CTGCTTCAGT GGATGAATCC 
AGACCTGAGT TTATCTGCAG GCTGGCTCTC GGCATATTCT CCGTCTCCGT GCTTCACATC 
GATCCTTTGT CTCCAGCGGA AACGTCTCTG AACGTCAACC CATTGACACG CATGGCCACC 
GATTTCTTCT CCTGTGTAGA AAAGATGGAC CCAGCAATAT TTTCGACGGG AGACTTTAAG 
TCTTTCCGAG CAGTCTTTGC CGAAGCTTGT TCACACGATC ACCTTAGGTT TATAGGTACT 
GGCATCAAAG TGTCCTATGA GCAGAGACAA AGGTCAGCTT CCCGGCACTT TAGCACCGAC 
ATGTCCGTTG GGCAAATGGA GTTTTTGGAG TGTCTGTTTC CAACAGATTG TCATTCTGTC 
CCTTCTCACT ACACCGAGCT TCTAACGTTC CATTCCAAAG AAGGGACGGA TGCCCACCTT 
CCCGTTTGTC TTCAGCTTCA CTATAAACAC TCAGAGACTA GAGGACCCCA GGGTAATCAA 
GCAAGACTTA GTTCAGTTCC TCAAAAGGCA GAATTACAAA TTAAATTAAA CCCCGTGTGT 
TGCGAGCTGG ATATCAGTAT TGTGGATAGG TTAAATTCCT TGCTTCAACC TCAGAAACTC 
ACTACCGTGG AGATGATGGC CTCGCACATG TATGCGTCCT ATAACAAGCA TATTAGTTTG 
CACAAGGCTT TCACGGAAGT GTTCCTAGAT GACTCACACA GTCCTGCAAA CCGTCGGGTG 
TCCGTACAAG TTGCCACCCC AGCCCTGCAC CTGTCTGTTC GCTTCCCTAT TCCTGATCTA 
AGATCAGACC AGGAAAGAGG ACCCTGGTTT AAGAAGTCAC TTCAGAAGGA GACCCTTCAT 
TTAGAATTTA CGGATCTGGA GTCCAAGACA GAGTTCGTGG GAGGATCCAC CCCAGAACAA 
ACTAAACTGG AGCTGACTTT TCGAGAGCTA TCTGGGTCGT TCCAGGAGGA GAAGGGAGGG 
CCATCTGTCA AGTTCTTCCA TGTGTCTGGT GGAGTAGATG GAGACACCGC ATCGTCAGAT 
GACTTTGACT GGCCACGAAT GGTACTGAAA ATAAACCCAC CAGCCATGCA TTCCATTTTG 
GAGAGAATAG CAGCCGAGGA AGAGGAAGAG AATGATGGTC ACTACCAGGA GGAGGAGGAC 
GGAGGGGCTC ATTCCCTGAA AGATGTCTGT GACCTGAGGC GGCCAGCCCC GTCTCCCTTT 
TCCTCTCGTC GAGTGATGTT TGAGAATGAG CAGATGGTGA TGCCGGGAGA CCCTGTGGAG 
ATGACGGAGT TTCAGGATAA AGCCATCAGC AACTCGCACT ACGTGCTGGA GCTCCTGTTG 
CCCAACATCC ACCTGACGCT GCCCAACAAA GGCTTCTACG AGAAGCTCTA TAACAGGATC 
TTTAACGACT TGCTTCTCTG GGAGCCAACA GCGCCTTCAC CAGTGGAGAC ACTTGAAAAC 
GTTTCCTATG GCATCGGACT CTCCGTGGCC AGTCAGCTGA TCAATACCTT CAGCAAAGAT 
AGCTTCAGTC CCTTTAAATC TGCAGTTCAC TATGATGAAG ACAGTGGATC TGAAGAAGAG 
ACTTTGCAGT ATTTTTCTGC TGTTGATCCC AACTATCGTT CCCGTAGGAA AAAAAAGTTG 
GACTCTCAGA ACAAGAACTC TCAGAGCTTC CTCTCAGTGC TTCTGAGTAT CAATCATGGA 
CTAATGGCAG TGTTCACTGA TGTGAAGCAA GAGAATGGAG ATCCGATGGA AAGTAAGCAT 
GGTGAATTCT GGCTAGAATT CAATAGCGGC TCGTTCTTCT GCGTGACAAA GTATGAGGGC 
TTTGAGGACA AGCACTACAT CTGCTTGCAT TCCAGCAGCC TCAGGCTGTA CCACAAAGGC 
ATAGTAGATG GAGCCATTCT CCCTACAGAG ACGCGCTTGC CCTGTTCCAC CCGCCCACAC 
TGGCTGGAAC CTACAATTTA TTCCTCTGAA GAAGATGGCC TCAGCCGCAC TGCCTCAGAT 
GGGGTTGGGG GAGACAATTT GAATATGCTC TCAGTCGCAG TGAAGATACT GTCTGATAAG 
TCCGAGTCCA ACACGAAGGA GTTCCTCGTG GCTGTGGGGC TGAAAGGAGC CACTCTACAG 
CACAGGGTGC TGCCCGCTGG ACTCAGTTGG CACGAGCAGA TTTTAAACTT CTTGAATATT 
GCTGATGAAC CTGTTTTGGG ATATAACCCT CCAACTTCAT TTACAACTTT CCATGTCCAT 
CTTTGGAGCT GTGCACTTGA TTACAGGCCC CTTCACCTGC CACTCCGATC CCTTCTTACT 
GTTGAGACGT TCAGCATTTC TAGCAGTGTT GCTTTGGATA AATCTTCCTC CACTCTCAGA 
ATCATCATGG ACGAGGCTGC TTTGCACCTG TCAGACAAGT GCAACACGGT CACTGTAAAC 
CTGAATAGAG ATTATGTTCG TGTGATGGAC ATGGGACTTT TAGAGCTGAC CATAACCGCA 
GTAAAATCTG ATTCTGATGG AGAACAGACT GCGCCCCGCT TTGAGTTACA CTGTTCCAGT 
GACGTCGTGC ACATCAGAAC ATGCTCAGAC TCATGTGCTG CGCTGATGAA CCTCATTCAG 
TATGTCGCAA GTTACGGGGA CCTACATGGA CCTCACAAAG CAGAGATGAA GCCTGGAGTC 
CCACAGAGAA AGCCCAAGGT AGATTCCAGC GCTCGGTCAT CTTCACATGG TCCAGTGCTT 
CCTGAAGCAG ATCAGCAGAT TTTACGGGAC CTGATGAGTG ATGCCATGGA GGAGATTGAT 
CTGCAGCAGG CCTCTGCCCC TGTCGGACCA CAGGCTAATG GTGTCCTGGA TGAAAAGTCT 
CACACTCAGG AGCCGTGCTG TTCAGACCTC TTCCTGTTCC CAGATGAGAG TGGAAATGTG 
TCCCAGGAGT CCAGCCCTGC CTATCCGTCT CTCACTCACC ACTTGATCAG TGACGCGGTG 
ACCGGTGTGA CCGCTGAAAA TGATGACTTC TGTATTCTTT TTGCACCAAA AACAGTCGTT 
CAGGAGAAGG AAGAAGAGCC AGTGATAAAG ATCATGGTTG ACGACGCCAT TGTGATAAAA 
GATGACTATT TCAGTCTGCC CATCACGAGG ACAGACAGCA GCAAAGCGCC GCTCCACTTT 
CCCATCCCTG CCGTCCGATA TGTTGTTAAA GAGGTCTCTC TGGTGTGGCA TCTTTATGGA 
GGCAAGGACT TTGCAACAGC TCCTCCAACT TCTCCAGCTA AGAGTTACAT TAGTCCCCAC 
AGCTCACCGT CTCAAACACC TACAAGGCAC GGGCGCCACA CAGTCTGTGG GGGCAAAGGA 
AGGAACCATG ACTTCTTGAT GGAAATACAG TTAAGCAAGG TGAAGTTTCA GCATGAGGTC 
TACCCTCCGT GTAAGCCTGA GTGTGAGTCC AGCCTCTTAG AACACCCAGT CTCCAGGCAG 
GTCTTCATCG TGCAGGACCT TGAAATTCGA GATCGGCTGG CAACATCACA GATGAATAAG 
TTTTTATACC TGTATTGCAG CAAAGACATG CCTCGTAAAG CCCATTCCAA CATGCTGACA 
ATTAAAGCGT TGCATGTACG TCCGGAGTCT GGCAGGTCAC CACAGGAGTG CTGCTTGAGA 
GTGTCCCTGA TGCCTCTTCG CCTCAATATT GACCAGGACG CCTTGTTCTT CCTCAAGGAT 
TTCTTCACAA GTCTTTCTAC AGAAGTGGAG CTTCTACTGA CTCCAGATCC AGAAGTTACA 
AAGTCTCCTG GAGCTGATGT CACCTGCAGT TTGCCAAGGC ATTTGAGTAC CTCAAAGGAG 
CCAAATCTAG TCGTTTCTTT TCCTGGGCCA AAACAAGCTT CCCCGAACCA TCGTGCCAAT 
TCAGCGGAGG GTGGTAATGG ACTGGAGGAG GACGTCTCTG CTGAGGAAAC TTCATTTAGT 
GACCAGCCTG TGTTTTTTAG AGAATTTAGA TTCACGGCAG AAGTTCCCAT CCGGCTTGAC 
TACCACGGCA AGCACGTGTC GATGGATCAG GGTACATTGG CTGGGATTTT GATTGGCCTG 
GCCCAGTTGA ACTGTTCGGA GCTAAAGCTG AAAAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTATTGACA AATTGTTCTC ATATGCAATC TCGGAATGGC TCAGTGACAT TAAGAAGAAC 
CAGCTACCAG GAATCCTGGG AGGTGTTGGC CCTATGCATT CACTAGTTCA ATTAGTGCAA 
GGCCTGAAGG ACTTGGTATG GCTGCCCATT GAGCAATACC GGAAGGATGG CCGTATCGTC 
AGAGGCTTCC AGAGGGGCGC TGCTTCCTTT GGTACCTCAA CTGCAATGGC TGCCCTGGAA 
CTCACAAACA GAATGGTCCA AACCATACAG GCAGCCGCAG AGACTGCATA TGACATGGTG 
TCTCCGAGTA CCCTTTCCAT TGAGCCCAAG AAGGCAAAGA GATTTCCTCA TCACCGGTTA 
GCCCACCAGC CAGTAGACCT GAGGGAAGGC GTGGCCAAGG CCTACAGCGT TGTAAAAGAG 
GGGATCACAG ACACAGCTCA GACCATCTAT GAAACGGCGG CTCGGGAACA CGAGAGCAGA 
GGGGTCACTG GTGCTGTGGG TGAAGTCCTG CGGCAGATCC CACCGGCAGT GGTCAGACCC 
CTGATTGTAG CCACCGAGGC GACATCAAAT GTGTTAGGTG GCATGAGAAA CCAAATTAGA 
CCAGACGTTC GGCAGGACGA GTCGCAGAAA TGGCGCCATG GGGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Macrophage autophagy in immunity to Cryptococcus neoformans and Candida albicans.
Infection and immunity80(9)3065-76(2012 Sep)
Nicola AM,Albuquerque P,Martinez LR,Dal-Rosso RA,Saylor C,De Jesus M,Nosanchuk JD,Casadevall A

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Mouse satellite DNA is transcribed in senescent cardiac muscle.
The Journal of biological chemistry265(29)17753-8(1990 Oct)
Gaubatz JW,Cutler RG

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Atg2b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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