Msrb2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Msrb2
Entrez Gene ID	76467
Full Name	methionine sulfoxide reductase B2
Synonyms	2310050L06Rik,Mrsb,Msrb,Pilb
General protein information	Preferred Names methionine sulfoxide reductase B2 Names methionine-R-sulfoxide reductase B2, mitochondrial pilin-like transcription factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006497563.1	XP_006497626.1	methionine-R-sulfoxide reductase B2, mitochondrial isoform X3
XM_017319320.2	XP_017174809.1	methionine-R-sulfoxide reductase B2, mitochondrial isoform X1
NM_029619.2	NP_083895.1	methionine-R-sulfoxide reductase B2, mitochondrial precursor
XM_017319321.1	XP_017174810.1	methionine-R-sulfoxide reductase B2, mitochondrial isoform X2
XM_017319320.1	XP_017174809.1	methionine-R-sulfoxide reductase B2, mitochondrial isoform X1
XM_006497563.2	XP_006497626.1	methionine-R-sulfoxide reductase B2, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Msrb2	NP_083895.1
Homo sapiens (human)	MSRB2	NP_036360.3
Bos taurus (cattle)	MSRB2	NP_001193059.1
Rattus norvegicus (Norway rat)	Msrb2	NP_001026830.1
Danio rerio (zebrafish)	msrb2	NP_998086.1
Xenopus tropicalis (tropical clawed frog)	msrb2	NP_001015905.1
Pan troglodytes (chimpanzee)	MSRB2	XP_001155268.2
	LOC711305	XP_001100084.2
Canis lupus familiaris (dog)	MSRB2	XP_850458.2
Caenorhabditis elegans (roundworm)	F44E2.6	NP_001254966.1

Gene Ontology
Bibliography

Related articles in PubMed

Methionine sulfoxide reductase B3 deficiency causes hearing loss due to stereocilia degeneration and apoptotic cell death in cochlear hair cells.
Kwon TJ, Cho HJ, Kim UK, Lee E, Oh SK, Bok J, Bae YC, Yi JK, Lee JW, Ryoo ZY, Lee SH, Lee KY, Kim HY
Human molecular genetics23(6)1591-601(2014 Mar)

MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation.
Lee BC, P?terfi Z, Hoffmann FW, Moore RE, Kaya A, Avanesov A, Tarrago L, Zhou Y, Weerapana E, Fomenko DE, Hoffmann PR, Gladyshev VN
Molecular cell51(3)397-404(2013 Aug)

Next-generation sequencing identifies the Danforth's short tail mouse mutation as a retrotransposon insertion affecting Ptf1a expression.
Vlangos CN, Siuniak AN, Robinson D, Chinnaiyan AM, Lyons RH Jr, Cavalcoli JD, Keegan CE
PLoS genetics9(2)e1003205(2013)

A retrotransposon insertion in the 5' regulatory domain of Ptf1a results in ectopic gene expression and multiple congenital defects in Danforth's short tail mouse.
Lugani F, Arora R, Papeta N, Patel A, Zheng Z, Sterken R, Singer RA, Caridi G, Mendelsohn C, Sussel L, Papaioannou VE, Gharavi AG
PLoS genetics9(2)e1003206(2013)

Analyses of methionine sulfoxide reductase activities towards free and peptidyl methionine sulfoxides.
Kwak GH, Hwang KY, Kim HY
Archives of biochemistry and biophysics527(1)1-5(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that glutaredoxin acts as a reductant for methionine sulfoxide reductases A and B (MsrA and MsrB) with or without resolving cysteine.
Title: Glutaredoxin serves as a reductant for methionine sulfoxide reductases with or without resolving cysteine.

overall structure is composed of two beta-sheets consisting of eight antiparallel beta-strands and three N-terminal alpha-helices and is more similar to those of bacterial MsrBs than to that of mammalian MsrB1
Title: Structural and biochemical analysis of mammalian methionine sulfoxide reductase B2.

MsrA and MsrB2, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies.
Title: Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging.

Results show that mammalian MsrB and MsrA proteins perform crucial functions in protection against oxidative stress in lower eukaryotic yeast cells.
Title: Expression, subcellular localization, and antioxidant role of mammalian methionine sulfoxide reductases in Saccharomyces cerevisiae.

Studies show all Met sulfoxide residues in an MRP can be reduced by MsrA and MsrB.
Title: Analysis of methionine/selenomethionine oxidation and methionine sulfoxide reductase function using methionine-rich proteins and antibodies against their oxidized forms.

The following Msrb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Msrb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07222	NM_029619.2 Latest version!	Mus musculus methionine sulfoxide reductase B2 (Msrb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu29210	XM_006497563.2 Latest version!	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu80453	XM_017319320.2 Latest version!	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu80454	XM_017319321.1 Latest version!	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu80453	XM_017319320.1	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu29210	XM_006497563.1	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Msrb2 ( NM_029619.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083895.1 Mus musculus methionine sulfoxide reductase B2 (Msrb2), mRNA.

Price & Availability↑

CloneID	OMu07222
Clone ID Related Accession (Same CDS sequence)	NM_029619.2
Accession Version	NM_029619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	methionine-R-sulfoxide reductase B2, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC021619.1. On Jan 15, 2003 this sequence version replaced NM_029619.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC021619.1, AK009912.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCGAC TGCTGCGAGC GCTGCGGGGT CTGCCTTTGC TGCAGGCGCC TGGACGGCTG 
GCTCGGGGTT GCGCGGGATC GGGAAGCAAG GACACAGGGT CTCTTACAAA GTCCAAGCGC 
TCTCTAAGCG AAGCAGACTG GCAGAAGAAA CTGACCCCGG AACAGTTCTA TGTCACCAGA 
GAGAAAGGAA CTGAAGCGCC TTTCAGTGGA ATGTATTTGA ACAACAAGGA GACAGGGATG 
TACCATTGTG TGTGCTGTGA TAGCCCACTC TTCAGTTCTG AGAAGAAGTA CTGTTCTGGC 
ACCGGCTGGC CTTCATTTTC TGAGGCTTAC GGCTCAAAAG GCTCAGATGA AAGTCACACA 
GGGATCCTGA GACGACTGGA CACTTCCTTA GGATGTCCTC GCATGGAGGT GGTCTGCAAG 
CAGTGTGAAG CTCACCTTGG CCATGTGTTT CCTGATGGAC CCAAGCCCAC TGGCCAAAGG 
TTTTGCATCA ACAGTGTGGC CCTGAAGTTC AAACCCAGCA AGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083895.1
CDS	48..575
Translation	MARLLRALRGLPLLQAPGRLARGCAGSGSKDTGSLTKSKRSLSEADWQKKLTPEQFYVTREKGTEAPFSGMYLNNKETGMYHCVCCDSPLFSSEKKYCSGTGWPSFSEAYGSKGSDESHTGILRRLDTSLGCPRMEVVCKQCEAHLGHVFPDGPKPTGQRFCINSVALKFKPSKP

Target ORF information:

RefSeq Version	NM_029619.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine sulfoxide reductase B2 (Msrb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029619.2

ORF Insert Sequence:

ATGGCGCGAC TGCTGCGAGC GCTGCGGGGT CTGCCTTTGC TGCAGGCGCC TGGACGGCTG 
GCTCGGGGTT GCGCGGGATC GGGAAGCAAG GACACAGGGT CTCTTACAAA GTCCAAGCGC 
TCTCTAAGCG AAGCAGACTG GCAGAAGAAA CTGACCCCGG AACAGTTCTA TGTCACCAGA 
GAGAAAGGAA CTGAAGCGCC TTTCAGTGGA ATGTATTTGA ACAACAAGGA GACAGGGATG 
TACCATTGTG TGTGCTGTGA TAGCCCACTC TTCAGTTCTG AGAAGAAGTA CTGTTCTGGC 
ACCGGCTGGC CTTCATTTTC TGAGGCTTAC GGCTCAAAAG GCTCAGATGA AAGTCACACA 
GGGATCCTGA GACGACTGGA CACTTCCTTA GGATGTCCTC GCATGGAGGT GGTCTGCAAG 
CAGTGTGAAG CTCACCTTGG CCATGTGTTT CCTGATGGAC CCAAGCCCAC TGGCCAAAGG 
TTTTGCATCA ACAGTGTGGC CCTGAAGTTC AAACCCAGCA AGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msrb2 ( XM_006497563.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497626.1 Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu29210
Clone ID Related Accession (Same CDS sequence)	XM_006497563.1 , XM_006497563.2
Accession Version	XM_006497563.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methionine-R-sulfoxide reductase B2, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGA ATGGGTCTCT TACAAAGTCC AAGCGCTCTC TAAGCGAAGC AGACTGGCAG 
AAGAAACTGA CCCCGGAACA GTTCTATGTC ACCAGAGAGA AAGGAACTGA AGCGCCTTTC 
AGTGGAATGT ATTTGAACAA CAAGGAGACA GGGATGTACC ATTGTGTGTG CTGTGATAGC 
CCACTCTTCA GTTCTGAGAA GAAGTACTGT TCTGGCACCG GCTGGCCTTC ATTTTCTGAG 
GCTTACGGCT CAAAAGGCTC AGATGAAAGT CACACAGGGA TCCTGAGACG ACTGGACACT 
TCCTTAGGAT GTCCTCGCAT GGAGGTGGTC TGCAAGCAGT GTGAAGCTCA CCTTGGCCAT 
GTGTTTCCTG ATGGACCCAA GCCCACTGGC CAAAGGTTTT GCATCAACAG TGTGGCCCTG 
AAGTTCAAAC CCAGCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497626.1
CDS	506..949
Translation	MGWNGSLTKSKRSLSEADWQKKLTPEQFYVTREKGTEAPFSGMYLNNKETGMYHCVCCDSPLFSSEKKYCSGTGWPSFSEAYGSKGSDESHTGILRRLDTSLGCPRMEVVCKQCEAHLGHVFPDGPKPTGQRFCINSVALKFKPSKP

Target ORF information:

RefSeq Version	XM_006497563.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497563.1

ORF Insert Sequence:

ATGGGGTGGA ATGGGTCTCT TACAAAGTCC AAGCGCTCTC TAAGCGAAGC AGACTGGCAG 
AAGAAACTGA CCCCGGAACA GTTCTATGTC ACCAGAGAGA AAGGAACTGA AGCGCCTTTC 
AGTGGAATGT ATTTGAACAA CAAGGAGACA GGGATGTACC ATTGTGTGTG CTGTGATAGC 
CCACTCTTCA GTTCTGAGAA GAAGTACTGT TCTGGCACCG GCTGGCCTTC ATTTTCTGAG 
GCTTACGGCT CAAAAGGCTC AGATGAAAGT CACACAGGGA TCCTGAGACG ACTGGACACT 
TCCTTAGGAT GTCCTCGCAT GGAGGTGGTC TGCAAGCAGT GTGAAGCTCA CCTTGGCCAT 
GTGTTTCCTG ATGGACCCAA GCCCACTGGC CAAAGGTTTT GCATCAACAG TGTGGCCCTG 
AAGTTCAAAC CCAGCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msrb2 ( XM_006497563.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497626.1 Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu29210
Clone ID Related Accession (Same CDS sequence)	XM_006497563.1 , XM_006497563.2
Accession Version	XM_006497563.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	methionine-R-sulfoxide reductase B2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGA ATGGGTCTCT TACAAAGTCC AAGCGCTCTC TAAGCGAAGC AGACTGGCAG 
AAGAAACTGA CCCCGGAACA GTTCTATGTC ACCAGAGAGA AAGGAACTGA AGCGCCTTTC 
AGTGGAATGT ATTTGAACAA CAAGGAGACA GGGATGTACC ATTGTGTGTG CTGTGATAGC 
CCACTCTTCA GTTCTGAGAA GAAGTACTGT TCTGGCACCG GCTGGCCTTC ATTTTCTGAG 
GCTTACGGCT CAAAAGGCTC AGATGAAAGT CACACAGGGA TCCTGAGACG ACTGGACACT 
TCCTTAGGAT GTCCTCGCAT GGAGGTGGTC TGCAAGCAGT GTGAAGCTCA CCTTGGCCAT 
GTGTTTCCTG ATGGACCCAA GCCCACTGGC CAAAGGTTTT GCATCAACAG TGTGGCCCTG 
AAGTTCAAAC CCAGCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497626.1
CDS	503..946
Translation	MGWNGSLTKSKRSLSEADWQKKLTPEQFYVTREKGTEAPFSGMYLNNKETGMYHCVCCDSPLFSSEKKYCSGTGWPSFSEAYGSKGSDESHTGILRRLDTSLGCPRMEVVCKQCEAHLGHVFPDGPKPTGQRFCINSVALKFKPSKP

Target ORF information:

RefSeq Version	XM_006497563.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497563.2

ORF Insert Sequence:

ATGGGGTGGA ATGGGTCTCT TACAAAGTCC AAGCGCTCTC TAAGCGAAGC AGACTGGCAG 
AAGAAACTGA CCCCGGAACA GTTCTATGTC ACCAGAGAGA AAGGAACTGA AGCGCCTTTC 
AGTGGAATGT ATTTGAACAA CAAGGAGACA GGGATGTACC ATTGTGTGTG CTGTGATAGC 
CCACTCTTCA GTTCTGAGAA GAAGTACTGT TCTGGCACCG GCTGGCCTTC ATTTTCTGAG 
GCTTACGGCT CAAAAGGCTC AGATGAAAGT CACACAGGGA TCCTGAGACG ACTGGACACT 
TCCTTAGGAT GTCCTCGCAT GGAGGTGGTC TGCAAGCAGT GTGAAGCTCA CCTTGGCCAT 
GTGTTTCCTG ATGGACCCAA GCCCACTGGC CAAAGGTTTT GCATCAACAG TGTGGCCCTG 
AAGTTCAAAC CCAGCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msrb2 ( XM_017319320.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174809.1 Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80453
Clone ID Related Accession (Same CDS sequence)	XM_017319320.2 , XM_017319320.1
Accession Version	XM_017319320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	methionine-R-sulfoxide reductase B2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAA TCGGAGTAAG GTACTGCGGG AGGAGGAGGC AGTGGAGTCC CGGGATATTC 
CACACACTCC GCGAAGATCA AGGTCAAGAG GAGCAAGAGG ATAAGTTAAG TGGTGGGGGT 
AGGGTGGAGC ACCAGAAGAA AAGGAGAGCC TGGAGGAGGG GAAGAGACCT GGGCAGAGGG 
GAGGGATCCG GCGGAGGGTG GGGGTTGGGT AGGCTTGGGA GGAAACCCGT TGGTGGCAGC 
GTCATGGCGC GACTGCTGCG AGCGCTGCGG GGTCTGCCTT TGCTGCAGGC GCCTGGACGG 
CTGGCTCGGG GTTGCGCGGG ATCGGGAAGC AAGGACACAG GGTCTCTTAC AAAGTCCAAG 
CGCTCTCTAA GCGAAGCAGA CTGGCAGAAG AAACTGACCC CGGAACAGTT CTATGTCACC 
AGAGAGAAAG GAACTGAAGC GCACACGGGG ACCACAGAGA AAGCTCTGAG CAGTTGGATG 
TGTTTAATGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174809.1
CDS	5..499
Translation	MIPIGVRYCGRRRQWSPGIFHTLREDQGQEEQEDKLSGGGRVEHQKKRRAWRRGRDLGRGEGSGGGWGLGRLGRKPVGGSVMARLLRALRGLPLLQAPGRLARGCAGSGSKDTGSLTKSKRSLSEADWQKKLTPEQFYVTREKGTEAHTGTTEKALSSWMCLMS

Target ORF information:

RefSeq Version	XM_017319320.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319320.2

ORF Insert Sequence:

ATGATTCCAA TCGGAGTAAG GTACTGCGGG AGGAGGAGGC AGTGGAGTCC CGGGATATTC 
CACACACTCC GCGAAGATCA AGGTCAAGAG GAGCAAGAGG ATAAGTTAAG TGGTGGGGGT 
AGGGTGGAGC ACCAGAAGAA AAGGAGAGCC TGGAGGAGGG GAAGAGACCT GGGCAGAGGG 
GAGGGATCCG GCGGAGGGTG GGGGTTGGGT AGGCTTGGGA GGAAACCCGT TGGTGGCAGC 
GTCATGGCGC GACTGCTGCG AGCGCTGCGG GGTCTGCCTT TGCTGCAGGC GCCTGGACGG 
CTGGCTCGGG GTTGCGCGGG ATCGGGAAGC AAGGACACAG GGTCTCTTAC AAAGTCCAAG 
CGCTCTCTAA GCGAAGCAGA CTGGCAGAAG AAACTGACCC CGGAACAGTT CTATGTCACC 
AGAGAGAAAG GAACTGAAGC GCACACGGGG ACCACAGAGA AAGCTCTGAG CAGTTGGATG 
TGTTTAATGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msrb2 ( XM_017319320.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174809.1 Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80453
Clone ID Related Accession (Same CDS sequence)	XM_017319320.2 , XM_017319320.1
Accession Version	XM_017319320.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methionine-R-sulfoxide reductase B2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAA TCGGAGTAAG GTACTGCGGG AGGAGGAGGC AGTGGAGTCC CGGGATATTC 
CACACACTCC GCGAAGATCA AGGTCAAGAG GAGCAAGAGG ATAAGTTAAG TGGTGGGGGT 
AGGGTGGAGC ACCAGAAGAA AAGGAGAGCC TGGAGGAGGG GAAGAGACCT GGGCAGAGGG 
GAGGGATCCG GCGGAGGGTG GGGGTTGGGT AGGCTTGGGA GGAAACCCGT TGGTGGCAGC 
GTCATGGCGC GACTGCTGCG AGCGCTGCGG GGTCTGCCTT TGCTGCAGGC GCCTGGACGG 
CTGGCTCGGG GTTGCGCGGG ATCGGGAAGC AAGGACACAG GGTCTCTTAC AAAGTCCAAG 
CGCTCTCTAA GCGAAGCAGA CTGGCAGAAG AAACTGACCC CGGAACAGTT CTATGTCACC 
AGAGAGAAAG GAACTGAAGC GCACACGGGG ACCACAGAGA AAGCTCTGAG CAGTTGGATG 
TGTTTAATGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174809.1
CDS	30..524
Translation	MIPIGVRYCGRRRQWSPGIFHTLREDQGQEEQEDKLSGGGRVEHQKKRRAWRRGRDLGRGEGSGGGWGLGRLGRKPVGGSVMARLLRALRGLPLLQAPGRLARGCAGSGSKDTGSLTKSKRSLSEADWQKKLTPEQFYVTREKGTEAHTGTTEKALSSWMCLMS

Target ORF information:

RefSeq Version	XM_017319320.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319320.1

ORF Insert Sequence:

ATGATTCCAA TCGGAGTAAG GTACTGCGGG AGGAGGAGGC AGTGGAGTCC CGGGATATTC 
CACACACTCC GCGAAGATCA AGGTCAAGAG GAGCAAGAGG ATAAGTTAAG TGGTGGGGGT 
AGGGTGGAGC ACCAGAAGAA AAGGAGAGCC TGGAGGAGGG GAAGAGACCT GGGCAGAGGG 
GAGGGATCCG GCGGAGGGTG GGGGTTGGGT AGGCTTGGGA GGAAACCCGT TGGTGGCAGC 
GTCATGGCGC GACTGCTGCG AGCGCTGCGG GGTCTGCCTT TGCTGCAGGC GCCTGGACGG 
CTGGCTCGGG GTTGCGCGGG ATCGGGAAGC AAGGACACAG GGTCTCTTAC AAAGTCCAAG 
CGCTCTCTAA GCGAAGCAGA CTGGCAGAAG AAACTGACCC CGGAACAGTT CTATGTCACC 
AGAGAGAAAG GAACTGAAGC GCACACGGGG ACCACAGAGA AAGCTCTGAG CAGTTGGATG 
TGTTTAATGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msrb2 ( XM_017319321.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174810.1 Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu80454
Clone ID Related Accession (Same CDS sequence)	XM_017319321.1
Accession Version	XM_017319321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methionine-R-sulfoxide reductase B2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAA TCGGAGTAAG GTACTGCGGG AGGAGGAGGC AGTGGAGTCC CGGGATATTC 
CACACACTCC GCGAAGATCA AGGTCAAGAG GAGCAAGAGG ATAAGTTAAG TGGTGGGGGT 
AGGGTGGAGC ACCAGAAGAA AAGGAGAGCC TGGAGGAGGG GAAGAGACCT GGGCAGAGGG 
GAGGGATCCG GCGGAGGGTG GGGGTTGGGT AGGCTTGGGA GGAAACCCGT TGGTGGCAGC 
GTCATGGCGC GACTGCTGCG AGCGCTGCGG GGTCTGCCTT TGCTGCAGGC GCCTGGACGG 
CTGGCTCGGG GTTGCGCGGG ATCGGGAAGC AAGGACACAG GGTCTCTTAC AAAGTCCAAG 
CGCTCTCTAA GCGAAGCAGA CTGGCAGAAG AAACTGACCC CGGAACAGTT CTATGTCACC 
AGAGAGAAAG GAACTGAAGC GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174810.1
CDS	17..463
Translation	MIPIGVRYCGRRRQWSPGIFHTLREDQGQEEQEDKLSGGGRVEHQKKRRAWRRGRDLGRGEGSGGGWGLGRLGRKPVGGSVMARLLRALRGLPLLQAPGRLARGCAGSGSKDTGSLTKSKRSLSEADWQKKLTPEQFYVTREKGTEAF

Target ORF information:

RefSeq Version	XM_017319321.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine sulfoxide reductase B2 (Msrb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319321.1

ORF Insert Sequence:

ATGATTCCAA TCGGAGTAAG GTACTGCGGG AGGAGGAGGC AGTGGAGTCC CGGGATATTC 
CACACACTCC GCGAAGATCA AGGTCAAGAG GAGCAAGAGG ATAAGTTAAG TGGTGGGGGT 
AGGGTGGAGC ACCAGAAGAA AAGGAGAGCC TGGAGGAGGG GAAGAGACCT GGGCAGAGGG 
GAGGGATCCG GCGGAGGGTG GGGGTTGGGT AGGCTTGGGA GGAAACCCGT TGGTGGCAGC 
GTCATGGCGC GACTGCTGCG AGCGCTGCGG GGTCTGCCTT TGCTGCAGGC GCCTGGACGG 
CTGGCTCGGG GTTGCGCGGG ATCGGGAAGC AAGGACACAG GGTCTCTTAC AAAGTCCAAG 
CGCTCTCTAA GCGAAGCAGA CTGGCAGAAG AAACTGACCC CGGAACAGTT CTATGTCACC 
AGAGAGAAAG GAACTGAAGC GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methionine sulfoxide reductase B3 deficiency causes hearing loss due to stereocilia degeneration and apoptotic cell death in cochlear hair cells.
Human molecular genetics23(6)1591-601(2014 Mar)
Kwon TJ,Cho HJ,Kim UK,Lee E,Oh SK,Bok J,Bae YC,Yi JK,Lee JW,Ryoo ZY,Lee SH,Lee KY,Kim HY

MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation.
Molecular cell51(3)397-404(2013 Aug)
Lee BC,P?terfi Z,Hoffmann FW,Moore RE,Kaya A,Avanesov A,Tarrago L,Zhou Y,Weerapana E,Fomenko DE,Hoffmann PR,Gladyshev VN

Next-generation sequencing identifies the Danforth's short tail mouse mutation as a retrotransposon insertion affecting Ptf1a expression.
PLoS genetics9(2)e1003205(2013)
Vlangos CN,Siuniak AN,Robinson D,Chinnaiyan AM,Lyons RH Jr,Cavalcoli JD,Keegan CE

A retrotransposon insertion in the 5' regulatory domain of Ptf1a results in ectopic gene expression and multiple congenital defects in Danforth's short tail mouse.
PLoS genetics9(2)e1003206(2013)
Lugani F,Arora R,Papeta N,Patel A,Zheng Z,Sterken R,Singer RA,Caridi G,Mendelsohn C,Sussel L,Papaioannou VE,Gharavi AG

Analyses of methionine sulfoxide reductase activities towards free and peptidyl methionine sulfoxides.
Archives of biochemistry and biophysics527(1)1-5(2012 Nov)
Kwak GH,Hwang KY,Kim HY