Knl1 cDNA ORF clone, Mus musculus(house mouse)

The following Knl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Knl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu84153 NM_001362732.1
Latest version!
Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu07220 XM_006500376.3
Latest version!
Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu07220 XM_006500377.4
Latest version!
Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu88036 XM_030252192.1
Latest version!
Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu07220 NM_029617.3
Latest version!
Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu07220 XM_006500377.3 Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu07220 NM_029617.2 Mus musculus kinetochore scaffold 1 (Knl1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu07220 XM_006500376.2 Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu84153
    Clone ID Related Accession (Same CDS sequence) NM_001362732.1
    Accession Version NM_001362732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Mus musculus(house mouse)
    Product kinetochore scaffold 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK009772.1, BC080815.1 and AL772264.8. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC080815.1, AK033132.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    GTAAGCTGTC TTGAGCCAAA AAATGTTCTT GAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349661.1
    CDS459..5297
    Translation

    Target ORF information:

    RefSeq Version NM_001362732.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    GTAAGCTGTC TTGAGCCAAA AAATGTTCTT GAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07220
    Clone ID Related Accession (Same CDS sequence) XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
    Accession Version XM_006500377.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product kinetochore scaffold 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500377.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006500440.1
    CDS101..6460
    Translation

    Target ORF information:

    RefSeq Version XM_006500377.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006500377.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07220
    Clone ID Related Accession (Same CDS sequence) XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
    Accession Version XM_006500376.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product kinetochore scaffold 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500376.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006500439.1
    CDS443..6802
    Translation

    Target ORF information:

    RefSeq Version XM_006500376.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006500376.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88036
    Clone ID Related Accession (Same CDS sequence) XM_030252192.1
    Accession Version XM_030252192.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product kinetochore scaffold 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAAAACAG AGAGAAATTC AGAAATTTCA GGTATGAACA CTTTGCTTTG TGCTCCAATT 
    CAGACTCAGA TGCAACAAAA GGAGTTCTCA ATTACAGACT GCAACCATGA AAGGAAACAT
    GCAAATGATC AGACAGTCAT CTTTTCAGAT GAAAACCAGA TGGATCTGAC AGCAAGTCAC
    ACTGTAATGA TTACCAAAGG TCTTTCAGAC TGTACCAAAA ATGAAAATTC TACAAAAATA
    GATACCACGT CCTTTCTGGA GAATTTAAAG CATCATGCTG CAAATTCAAG AATTAAAAAG
    GATTTAGCAT GTTCCACAGT TTCCTTAAGT CAAAACATTT TCTCAGAAAA GATCAATTCT
    GACAACTTCA TAAAAAGATT GAAGACAGGA AAACATATTT CGTCTTCTAC AGAGCTTGAT
    AAGGAAAATG CTGAGATACC TGTTTATTCC AAAGACTCAA ACAGTGCCTC TTCAACATAT
    CAAATGCATG CATCTCTTGG TGTAGATGAA AATAGTAGTA ATAGGACTAG AATCTTTAGA
    GAACAAGATG ATGGTATGAA TTTGACCCAA TGTCATACAG CCTGTATTAA GACTTGGATT
    CCTCCATCCA CGGAGGCCAA AATAGGAGAA TTCAAAGGTG ATAAAACAAT TTATGGCAAT
    GAGTGTATGG AGTTGACAAC TAACTATACG ATACAGGTTT TGTCATCAGA AAATAATTTA
    TCTGAAAGAG AAACTCAAAC TCAAAATGGT ATGAATGTTA CAACAGTTGA TGGAGCTACA
    GCTCCAGCTC CAGAGAAGAA AACAGCCCTT AAGGATAAAC TAAATGCTGC TTTTCAAGGC
    TCTTTCCCAA ACCCTGAAAA TAAAATTCAT ATTATCAAAT GTCATCCTAT AGAGTCAGAA
    ACTCATACAG TCACACAGAT TTCTAGTCAA AGTGCCAGCA CATTGGCTGT AACTTCAAAA
    TCTATATGTT CTAGTCCAGC TATTGAGGGG TATAAGACAA TTTTTCATTC TAGTAGCAAT
    GATGCAATGG AACTGACTAA GTGTCTATCA GCTATGGAAG AGGAAAAAAA ATTGCTGAAG
    GCTGATGATA AGTATTCTAA AATATGTACC AATCCAGATG CTGGCCCACT CAGAGAGAAA
    ACTATTTATT TAGAAGAGGA TAGCATGGAC ATTACCAAAA GTCACACCGT TGCAATAGAT
    AATAAAATAT TTAAACATGA TCAAGAAAAC ATTAAAAAAG AAATAATAGC CATACCAATA
    TTTGAAAAGG AAATGGTGCT CCGAAATCTT ATGCCTATGT CAAAAGATGA GAAAAGGGAT
    GTAAATTATA TTTCAGTTCC TCAAGTATCT AAGGAAAGCT TACAACGGAG TCAGACAAAC
    ACTTTATCTG TTTCATTGAC TGACAAAAAG ATGGAATTCT TGGCAGATGA AGATATGGAT
    CTGACTAAAA GTCACACAAC TAAGTTAAGT CAAGTTATTC CTACAACTTT TGACTTAGCA
    TCAAAGAATG TCACCAAATC ATACTCTCAC AGCAAAAGCC CTTTAAATGA GTGGGAAAGC
    CTGGATAAAC AAGTAGTGCT AGGCCAGCAT TCTAAACTTC CTTTACCACA GAGGAAAGAT
    AGAGATGATC CTGACTGTTC TCATCATAAG ATAATGTATT CAGAGGAGTT ACAAACTATG
    GATCTAACCA AGAGCCACAC TATTGTAATT GGCTTTGGTC CTTCTGAAGT ACAAGAACAT
    AGTAAAATTA ATTTAGAACA CAAAAACAGC CAACTTACAG CAGAAAGCAT ACAAACAGCT
    GTGAACGTTC CTGCAGCAAA CTCTAGAGTA GTAACAACTA ATGATATGGA CATGTTAAAG
    GACAGAAGTA CACATAAGCC AGAATTGCTG AAGGAAAAGC AAAACATCAA AATCTATGGA
    AGAAAAAGTA TTGGTAGACT GAAAATTGAT AAAACTATAT TATTTTCAGA GGGTAATGAG
    GGTGATATGG ATATCACCAA GAGTTGCACA GTCAAAATAA ATCATAGATC TTTATTAGAT
    AAGCATGATT CTCATTTAGT ATCTTTGGCA GGAACTTCTA AAACCATTTT GCATGCACGT
    GGTCAGGTTG AAATGGAAAT CAATAGAAGT CACACAACTC CTTTAGAATG TAAAATTATC
    TCGCCCAGTG ATATAACTCC TGGTGATCTG GATAAGACTA TGATGTCTAT AGATGATCAT
    GAGGAACTAG ACATGACCAA GTCCCATACT GTTTTCATTG ACTACCAAGC AGAGGCAAAA
    GGTGTACTTC CAGACAGACT TGACTTTCAA TTATCCAAAA AGGAAAGCCT ACAGAAGCCG
    AAAGTGACAT CTCTGGCTGA AGAGATTTAC ATTTCAAAAA ATAGTGAAAG CAACCATCTA
    CCAGCAAAAG GCAGCCAGCT AACAATATTA GAGGAAGGGT CTAATAGCGG GCTTGGAGAG
    GAAACTAATG ATGCACAAAA GCCTGGATTT CTGAATGAAC TTCTGTCAGG CAAAACTCAG
    AGAAGAAAAA GCCTTAGCCT CAAAAACAAG AGTATTACAT TTCCAGAGAA TGATAAAAGT
    TATAGAGAGA TCCCTCAGAG TTCTGCAGTA GAAATAAATA ATGAAACTAG GCTGGAAGAC
    AGAAAGGGCT TCAGTTTTGT GCCATTGGCA GGAACTTCCA AACCAGTTTT GTCTGCATAT
    GGGCCCGAGG ACATGGAGAT TTCTATAGGT CAAACCACTG CTTCAGAATA TAAAACTGTC
    CCACCAGAAG AAATAACTAC TATACCTATG GATAAAACTG TAATGTTTGT AGATAATTTC
    GGTGATCTGG ATGTCACCAG ATCCCATACG GTTTTCATCG ATTGTCAAGC CAAAGAAAAG
    GTACTTGATG AATATACTAA CTTGGGAATA CAGAAAACAA AAACTTTGAG TGGTTCTGAA
    GGTGATACCC ATATTCAAGA AATCACAAAA AACCCAGCAG CTCAACACAA ACACCATATG
    ACAACTGTTA TTCCTTCTAG TACAGTAGTT AGTGATCAAA GTTCAATGAA AATCAAGTTT
    CACAAAGCTG ATAGAGATGA GGAGGTCAAA GGAAAGGAAG TGGAAGCCAA TATGCTGAAA
    CAAACTAAAC CTGAAAGCTG TCTGTTAAAT ATAACAGATG GAAAAAATGT GGATTTTACA
    AGCAGTTATA CAGCTGATGT TTGTAGATCC AGTGACAAGT ATTCCTCTTT ACCAAATATT
    TCTTCTTCTG ATAATTCGGG TGGTAATACT ATGTCCTTGT GTGATAAAAA TAAGGAGAAA
    GCTTATAATT GCCAAGTGCC AAATGAATTT ACTTATGCAG CTATCTTACC AAGTACATAT
    CACATGGATT CTAAAAAACT GTCTGTCTTT CCACCTTGTC CTTCAAAAGA AGTCACTCAA
    ACTGAAAGTG CCATAGCGCT GCTTAAAGAT GAAGATCCGG TAGAAGAGCC TCTGGGGGAG
    ATGGCTACAT TTAACTCAAA GCATGTATCT CTTAACCTGG CAAAGGATCA AACAGAGGCC
    TTTGTTGATG TTAGTGTTGC TTCTCAGCCT CATCTCTCAG CCCAACAAAG TCCATCAACT
    CAAAAAGGGC AGGATGTTGC CCGTAGAGAT GAAGGAATAT TAGCTAAAGC TGGGAAGAAG
    GCTTTACCTT TTCTTCTAGA AAATGTCGCT GCATCCACAT GGGAAAATGA GTCCAAAATA
    CCCACTAATG TGGAACACTT TGCTGTAACC TATGAAAAAG AACTGAGTAT AAGTATTCAA
    ACAGATAAAT GTAATACAAA TGTACAGAGC CCTAGTAACT CGGCCCTGAC AACACAAGTC
    ATTCAGACGC ATGCAAATGC TGAAGGAGCA CTAGACTTTT TAGTTCCATC TACTGTTTCA
    TGTTTTAGTA GTACCAAACC AAGTCTGAGT AATTTGAATA GAAAAACTGA GGAGGTCTTA
    GACTTTCAGA CTGTTAATTT ATTACCTCCT GCCGAACAGT TACTTGAAGA AGGAAGTCAG
    GCACATAGTA TGAGTATAGT ACAAGCTACA GAAATATATA GACTAGGTTC CAGGAATGAT
    AGAGATGAAG AAAGTAAAAC TTTCTGTAAT GAGGCTGAAA CTACATCTGT ACCACTAAAA
    ACAGCTGTTA AAGACAAAAC AAGAAGATGT TCATTGGGGA TATTTTTACC CAAATTACCA
    AGCAAGAGAA GTTGTAGTAT CACTGGTGTT GATGACCTGG AACAGATTCT AGCAGATGCA
    GCTGACTTAA CTCAACTAGA AACTCAGCCA GTCTGTAGTA AAGATCCAGG CATTGGATCT
    GTTGCAGCCA AACTGAATTT GAGTCCATCT CAGTTTATAA ATGAGGAAAA TCTCCCTGTA
    TATCCTGGTG AGATACTTTC ATCAGATTCT GTTAGCCTGG ACATTGAGGA AAGTGTTCTG
    ATTGACACAT CTCAGAGAGA GAGTTTACCG TCTGAAAACA AAACCGAAAA CTGCAGGGCC
    CAAAAAAGAA CCCGGGTTGA AGAAAATGAT GTTACAAATG AGAAAAAAAT CAGAACACAT
    GACAGTGCAC AAGATCAAGA GATTTTTGAT AATCATATGG AAGAGGATAT CAATAAAAAT
    GTCAACAGTG TATTGCTGAA AAGCCTGAGC AGGACACCGT CTAGTTGCAG CAGCTCTCTG
    GATTCAATCA AGTCTGATGG ACTCTCTCTG GATGTCAGCA CACAGCGTAA TAGCCAAATG
    GAATCACAGT TTCTTGGTGA CACTATCTCT GAAGAGAGTT TGAAGGAGAA ACTCAAGGAT
    GGACAAATTA CAATAAAGGA GTTCTTCATA CTTCTTCAGG TCCACATCTT AATACAGAAA
    CCTAGGCAGA GCACTCTCCC AGCCAAATTT ACTATAAACA CATTACCTAC CACAGAAGAT
    CTGATGTTGA GACAATATGT TTATGGACCC AGGATACAGA TTTATAAAGA AGACTGTGAG
    CTTCTTCGAC AAAAGATTGA CGAATTAAAG ATATCTGCAT TGAACCAAAA TAAACTATTG
    GCGGATGTAA ATAGGAACTT GTGGGAAAAA GTGAAAGACT ACTCTGATGA AGAGCTGAAG
    AACTATGGGA TTTACCTTAA CAAAATCAAA TCACGTTATA CCAAGATGAC AAAAGTCTTC
    AATCACCAAG GAAAAGTGGC TCTGTATAAC AAATTGGTGC ATTCAGCTGA GAATGAGAAG
    AACAAACTTC AGATAAAAAT AAATGAGATG GACACTATCC TCAAGAAGAT CAATAACTGT
    CTCGCTGAAG TGGAAACAGA AACTAAGAAT TTGGAGAATG AAGAGAAAAA TGATGCCATG
    GAAGAATGGG ATTCTGAAAT GAGAGATGCA GAGAAAGAAC TGGAACAGCT GAAAACTGAA
    GAAGAGGAGC TTCAGAGAAA GTTCTTAGAA GTAGAGACAC AGAAAACGCA GACCCTTGCT
    CAGATAGAAT TTATAAAAGA ACAAACAACT AAAACTGAAG AATTACTGGA TCAATTGAGT
    TTGTCTGAGT GGGATGTCAT TGAGTGGAGT GATGATCAAG CTGTATTTAC CTTTGTCTAT
    GATTCAATAG AACTCATCAT CACCTTTGGA GAGCCACTAG TTGGTCTCCC TTTTTTGGAC
    AAGGCTTGCA GGAAGATTAA TGCACTCAGT TTTCAGTCTC TGTTAGATGA AGATAAAGCT
    CCTCCCTCCT CCCTTTTAGT TCACAAGCTT ATTTTTCAGT ACATTGAGGA ACAGGAATCC
    TGGAAGAAGA AATGCACAGC ACAGCATCAG GTGCCCCAGA TGCTTCAAGA ACTCTCACTG
    GTAGTGAACC ATTGTAGGCT TCTTGGAGAG GAGATCGAAT TTTTAAAGCG ATGGGGGCCA
    AATTATAGCC TAATGCATAT AAATGTGAAT AATACTGAAT TGAGGCTTTT ATTCTCCAGT
    TGTGCAGCAT TTGCAAAGTT TGAGATAACT TTGTCTCCCT CGGCCCATTA TCCCTTGGTC
    CCGTTACCGT TCACCATTCA CAATCACATT GGGAAGACTG GCCATGATGA AATTGCTGCA
    ATTATCTCTA AAGTGCCCTT AGAGGAAAAC TACCTGAAGA ATGTAGTCAA GCAAATTTAC
    CAAGATCTAC TTAAGGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030108052.1
    CDS599..6739
    Translation

    Target ORF information:

    RefSeq Version XM_030252192.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore scaffold 1 (Knl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030252192.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAAAACAG AGAGAAATTC AGAAATTTCA GGTATGAACA CTTTGCTTTG TGCTCCAATT 
    CAGACTCAGA TGCAACAAAA GGAGTTCTCA ATTACAGACT GCAACCATGA AAGGAAACAT
    GCAAATGATC AGACAGTCAT CTTTTCAGAT GAAAACCAGA TGGATCTGAC AGCAAGTCAC
    ACTGTAATGA TTACCAAAGG TCTTTCAGAC TGTACCAAAA ATGAAAATTC TACAAAAATA
    GATACCACGT CCTTTCTGGA GAATTTAAAG CATCATGCTG CAAATTCAAG AATTAAAAAG
    GATTTAGCAT GTTCCACAGT TTCCTTAAGT CAAAACATTT TCTCAGAAAA GATCAATTCT
    GACAACTTCA TAAAAAGATT GAAGACAGGA AAACATATTT CGTCTTCTAC AGAGCTTGAT
    AAGGAAAATG CTGAGATACC TGTTTATTCC AAAGACTCAA ACAGTGCCTC TTCAACATAT
    CAAATGCATG CATCTCTTGG TGTAGATGAA AATAGTAGTA ATAGGACTAG AATCTTTAGA
    GAACAAGATG ATGGTATGAA TTTGACCCAA TGTCATACAG CCTGTATTAA GACTTGGATT
    CCTCCATCCA CGGAGGCCAA AATAGGAGAA TTCAAAGGTG ATAAAACAAT TTATGGCAAT
    GAGTGTATGG AGTTGACAAC TAACTATACG ATACAGGTTT TGTCATCAGA AAATAATTTA
    TCTGAAAGAG AAACTCAAAC TCAAAATGGT ATGAATGTTA CAACAGTTGA TGGAGCTACA
    GCTCCAGCTC CAGAGAAGAA AACAGCCCTT AAGGATAAAC TAAATGCTGC TTTTCAAGGC
    TCTTTCCCAA ACCCTGAAAA TAAAATTCAT ATTATCAAAT GTCATCCTAT AGAGTCAGAA
    ACTCATACAG TCACACAGAT TTCTAGTCAA AGTGCCAGCA CATTGGCTGT AACTTCAAAA
    TCTATATGTT CTAGTCCAGC TATTGAGGGG TATAAGACAA TTTTTCATTC TAGTAGCAAT
    GATGCAATGG AACTGACTAA GTGTCTATCA GCTATGGAAG AGGAAAAAAA ATTGCTGAAG
    GCTGATGATA AGTATTCTAA AATATGTACC AATCCAGATG CTGGCCCACT CAGAGAGAAA
    ACTATTTATT TAGAAGAGGA TAGCATGGAC ATTACCAAAA GTCACACCGT TGCAATAGAT
    AATAAAATAT TTAAACATGA TCAAGAAAAC ATTAAAAAAG AAATAATAGC CATACCAATA
    TTTGAAAAGG AAATGGTGCT CCGAAATCTT ATGCCTATGT CAAAAGATGA GAAAAGGGAT
    GTAAATTATA TTTCAGTTCC TCAAGTATCT AAGGAAAGCT TACAACGGAG TCAGACAAAC
    ACTTTATCTG TTTCATTGAC TGACAAAAAG ATGGAATTCT TGGCAGATGA AGATATGGAT
    CTGACTAAAA GTCACACAAC TAAGTTAAGT CAAGTTATTC CTACAACTTT TGACTTAGCA
    TCAAAGAATG TCACCAAATC ATACTCTCAC AGCAAAAGCC CTTTAAATGA GTGGGAAAGC
    CTGGATAAAC AAGTAGTGCT AGGCCAGCAT TCTAAACTTC CTTTACCACA GAGGAAAGAT
    AGAGATGATC CTGACTGTTC TCATCATAAG ATAATGTATT CAGAGGAGTT ACAAACTATG
    GATCTAACCA AGAGCCACAC TATTGTAATT GGCTTTGGTC CTTCTGAAGT ACAAGAACAT
    AGTAAAATTA ATTTAGAACA CAAAAACAGC CAACTTACAG CAGAAAGCAT ACAAACAGCT
    GTGAACGTTC CTGCAGCAAA CTCTAGAGTA GTAACAACTA ATGATATGGA CATGTTAAAG
    GACAGAAGTA CACATAAGCC AGAATTGCTG AAGGAAAAGC AAAACATCAA AATCTATGGA
    AGAAAAAGTA TTGGTAGACT GAAAATTGAT AAAACTATAT TATTTTCAGA GGGTAATGAG
    GGTGATATGG ATATCACCAA GAGTTGCACA GTCAAAATAA ATCATAGATC TTTATTAGAT
    AAGCATGATT CTCATTTAGT ATCTTTGGCA GGAACTTCTA AAACCATTTT GCATGCACGT
    GGTCAGGTTG AAATGGAAAT CAATAGAAGT CACACAACTC CTTTAGAATG TAAAATTATC
    TCGCCCAGTG ATATAACTCC TGGTGATCTG GATAAGACTA TGATGTCTAT AGATGATCAT
    GAGGAACTAG ACATGACCAA GTCCCATACT GTTTTCATTG ACTACCAAGC AGAGGCAAAA
    GGTGTACTTC CAGACAGACT TGACTTTCAA TTATCCAAAA AGGAAAGCCT ACAGAAGCCG
    AAAGTGACAT CTCTGGCTGA AGAGATTTAC ATTTCAAAAA ATAGTGAAAG CAACCATCTA
    CCAGCAAAAG GCAGCCAGCT AACAATATTA GAGGAAGGGT CTAATAGCGG GCTTGGAGAG
    GAAACTAATG ATGCACAAAA GCCTGGATTT CTGAATGAAC TTCTGTCAGG CAAAACTCAG
    AGAAGAAAAA GCCTTAGCCT CAAAAACAAG AGTATTACAT TTCCAGAGAA TGATAAAAGT
    TATAGAGAGA TCCCTCAGAG TTCTGCAGTA GAAATAAATA ATGAAACTAG GCTGGAAGAC
    AGAAAGGGCT TCAGTTTTGT GCCATTGGCA GGAACTTCCA AACCAGTTTT GTCTGCATAT
    GGGCCCGAGG ACATGGAGAT TTCTATAGGT CAAACCACTG CTTCAGAATA TAAAACTGTC
    CCACCAGAAG AAATAACTAC TATACCTATG GATAAAACTG TAATGTTTGT AGATAATTTC
    GGTGATCTGG ATGTCACCAG ATCCCATACG GTTTTCATCG ATTGTCAAGC CAAAGAAAAG
    GTACTTGATG AATATACTAA CTTGGGAATA CAGAAAACAA AAACTTTGAG TGGTTCTGAA
    GGTGATACCC ATATTCAAGA AATCACAAAA AACCCAGCAG CTCAACACAA ACACCATATG
    ACAACTGTTA TTCCTTCTAG TACAGTAGTT AGTGATCAAA GTTCAATGAA AATCAAGTTT
    CACAAAGCTG ATAGAGATGA GGAGGTCAAA GGAAAGGAAG TGGAAGCCAA TATGCTGAAA
    CAAACTAAAC CTGAAAGCTG TCTGTTAAAT ATAACAGATG GAAAAAATGT GGATTTTACA
    AGCAGTTATA CAGCTGATGT TTGTAGATCC AGTGACAAGT ATTCCTCTTT ACCAAATATT
    TCTTCTTCTG ATAATTCGGG TGGTAATACT ATGTCCTTGT GTGATAAAAA TAAGGAGAAA
    GCTTATAATT GCCAAGTGCC AAATGAATTT ACTTATGCAG CTATCTTACC AAGTACATAT
    CACATGGATT CTAAAAAACT GTCTGTCTTT CCACCTTGTC CTTCAAAAGA AGTCACTCAA
    ACTGAAAGTG CCATAGCGCT GCTTAAAGAT GAAGATCCGG TAGAAGAGCC TCTGGGGGAG
    ATGGCTACAT TTAACTCAAA GCATGTATCT CTTAACCTGG CAAAGGATCA AACAGAGGCC
    TTTGTTGATG TTAGTGTTGC TTCTCAGCCT CATCTCTCAG CCCAACAAAG TCCATCAACT
    CAAAAAGGGC AGGATGTTGC CCGTAGAGAT GAAGGAATAT TAGCTAAAGC TGGGAAGAAG
    GCTTTACCTT TTCTTCTAGA AAATGTCGCT GCATCCACAT GGGAAAATGA GTCCAAAATA
    CCCACTAATG TGGAACACTT TGCTGTAACC TATGAAAAAG AACTGAGTAT AAGTATTCAA
    ACAGATAAAT GTAATACAAA TGTACAGAGC CCTAGTAACT CGGCCCTGAC AACACAAGTC
    ATTCAGACGC ATGCAAATGC TGAAGGAGCA CTAGACTTTT TAGTTCCATC TACTGTTTCA
    TGTTTTAGTA GTACCAAACC AAGTCTGAGT AATTTGAATA GAAAAACTGA GGAGGTCTTA
    GACTTTCAGA CTGTTAATTT ATTACCTCCT GCCGAACAGT TACTTGAAGA AGGAAGTCAG
    GCACATAGTA TGAGTATAGT ACAAGCTACA GAAATATATA GACTAGGTTC CAGGAATGAT
    AGAGATGAAG AAAGTAAAAC TTTCTGTAAT GAGGCTGAAA CTACATCTGT ACCACTAAAA
    ACAGCTGTTA AAGACAAAAC AAGAAGATGT TCATTGGGGA TATTTTTACC CAAATTACCA
    AGCAAGAGAA GTTGTAGTAT CACTGGTGTT GATGACCTGG AACAGATTCT AGCAGATGCA
    GCTGACTTAA CTCAACTAGA AACTCAGCCA GTCTGTAGTA AAGATCCAGG CATTGGATCT
    GTTGCAGCCA AACTGAATTT GAGTCCATCT CAGTTTATAA ATGAGGAAAA TCTCCCTGTA
    TATCCTGGTG AGATACTTTC ATCAGATTCT GTTAGCCTGG ACATTGAGGA AAGTGTTCTG
    ATTGACACAT CTCAGAGAGA GAGTTTACCG TCTGAAAACA AAACCGAAAA CTGCAGGGCC
    CAAAAAAGAA CCCGGGTTGA AGAAAATGAT GTTACAAATG AGAAAAAAAT CAGAACACAT
    GACAGTGCAC AAGATCAAGA GATTTTTGAT AATCATATGG AAGAGGATAT CAATAAAAAT
    GTCAACAGTG TATTGCTGAA AAGCCTGAGC AGGACACCGT CTAGTTGCAG CAGCTCTCTG
    GATTCAATCA AGTCTGATGG ACTCTCTCTG GATGTCAGCA CACAGCGTAA TAGCCAAATG
    GAATCACAGT TTCTTGGTGA CACTATCTCT GAAGAGAGTT TGAAGGAGAA ACTCAAGGAT
    GGACAAATTA CAATAAAGGA GTTCTTCATA CTTCTTCAGG TCCACATCTT AATACAGAAA
    CCTAGGCAGA GCACTCTCCC AGCCAAATTT ACTATAAACA CATTACCTAC CACAGAAGAT
    CTGATGTTGA GACAATATGT TTATGGACCC AGGATACAGA TTTATAAAGA AGACTGTGAG
    CTTCTTCGAC AAAAGATTGA CGAATTAAAG ATATCTGCAT TGAACCAAAA TAAACTATTG
    GCGGATGTAA ATAGGAACTT GTGGGAAAAA GTGAAAGACT ACTCTGATGA AGAGCTGAAG
    AACTATGGGA TTTACCTTAA CAAAATCAAA TCACGTTATA CCAAGATGAC AAAAGTCTTC
    AATCACCAAG GAAAAGTGGC TCTGTATAAC AAATTGGTGC ATTCAGCTGA GAATGAGAAG
    AACAAACTTC AGATAAAAAT AAATGAGATG GACACTATCC TCAAGAAGAT CAATAACTGT
    CTCGCTGAAG TGGAAACAGA AACTAAGAAT TTGGAGAATG AAGAGAAAAA TGATGCCATG
    GAAGAATGGG ATTCTGAAAT GAGAGATGCA GAGAAAGAAC TGGAACAGCT GAAAACTGAA
    GAAGAGGAGC TTCAGAGAAA GTTCTTAGAA GTAGAGACAC AGAAAACGCA GACCCTTGCT
    CAGATAGAAT TTATAAAAGA ACAAACAACT AAAACTGAAG AATTACTGGA TCAATTGAGT
    TTGTCTGAGT GGGATGTCAT TGAGTGGAGT GATGATCAAG CTGTATTTAC CTTTGTCTAT
    GATTCAATAG AACTCATCAT CACCTTTGGA GAGCCACTAG TTGGTCTCCC TTTTTTGGAC
    AAGGCTTGCA GGAAGATTAA TGCACTCAGT TTTCAGTCTC TGTTAGATGA AGATAAAGCT
    CCTCCCTCCT CCCTTTTAGT TCACAAGCTT ATTTTTCAGT ACATTGAGGA ACAGGAATCC
    TGGAAGAAGA AATGCACAGC ACAGCATCAG GTGCCCCAGA TGCTTCAAGA ACTCTCACTG
    GTAGTGAACC ATTGTAGGCT TCTTGGAGAG GAGATCGAAT TTTTAAAGCG ATGGGGGCCA
    AATTATAGCC TAATGCATAT AAATGTGAAT AATACTGAAT TGAGGCTTTT ATTCTCCAGT
    TGTGCAGCAT TTGCAAAGTT TGAGATAACT TTGTCTCCCT CGGCCCATTA TCCCTTGGTC
    CCGTTACCGT TCACCATTCA CAATCACATT GGGAAGACTG GCCATGATGA AATTGCTGCA
    ATTATCTCTA AAGTGCCCTT AGAGGAAAAC TACCTGAAGA ATGTAGTCAA GCAAATTTAC
    CAAGATCTAC TTAAGGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07220
    Clone ID Related Accession (Same CDS sequence) XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
    Accession Version NM_029617.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Mus musculus(house mouse)
    Product kinetochore scaffold 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772264.8. On Apr 26, 2018 this sequence version replaced NM_029617.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_083893.2
    CDS122..6481
    Translation

    Target ORF information:

    RefSeq Version NM_029617.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_029617.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07220
    Clone ID Related Accession (Same CDS sequence) XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
    Accession Version NM_029617.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Mus musculus(house mouse)
    Product kinetochore scaffold 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL772264.8. On or before Feb 10, 2007 this sequence version replaced NM_001081042.1, NM_029617.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_083893.2
    CDS122..6481
    Translation

    Target ORF information:

    RefSeq Version NM_029617.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore scaffold 1 (Knl1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_029617.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07220
    Clone ID Related Accession (Same CDS sequence) XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
    Accession Version XM_006500376.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein CASC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006500376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006500439.1
    CDS540..6899
    Translation

    Target ORF information:

    RefSeq Version XM_006500376.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006500376.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07220
    Clone ID Related Accession (Same CDS sequence) XM_006500376.2 , XM_006500377.4 , NM_029617.3 , XM_006500377.3 , NM_029617.2 , XM_006500376.3
    Accession Version XM_006500377.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein CASC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006500440.1
    CDS144..6503
    Translation

    Target ORF information:

    RefSeq Version XM_006500377.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus cancer susceptibility candidate 5 (Casc5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006500377.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGATGGGG TATATTCAGA AGCTAATGAA GAGAATGACA ATACACAGAG ACCTGTTAGA 
    AGACAGCATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT CAAATGTGGA
    AATCAAACAA ACCAGGAACC TAATCCTCCA AGAAAAAGGA AAAGCTCTCG TCGAGTCAGC
    TTTGCAGATA CTATAAAAGT ATTCCAGACA GAGTCTCACA TGAAAACAGA GAGAAATTCA
    GAAATTTCAG GTATGAACAC TTTGCTTTGT GCTCCAATTC AGACTCAGAT GCAACAAAAG
    GAGTTCTCAA TTACAGACTG CAACCATGAA AGGAAACATG CAAATGATCA GACAGTCATC
    TTTTCAGATG AAAACCAGAT GGATCTGACA GCAAGTCACA CTGTAATGAT TACCAAAGGT
    CTTTCAGACT GTACCAAAAA TGAAAATTCT ACAAAAATAG ATACCACGTC CTTTCTGGAG
    AATTTAAAGC ATCATGCTGC AAATTCAAGA ATTAAAAAGG ATTTAGCATG TTCCACAGTT
    TCCTTAAGTC AAAACATTTT CTCAGAAAAG ATCAATTCTG ACAACTTCAT AAAAAGATTG
    AAGACAGGAA AACATATTTC GTCTTCTACA GAGCTTGATA AGGAAAATGC TGAGATACCT
    GTTTATTCCA AAGACTCAAA CAGTGCCTCT TCAACATATC AAATGCATGC ATCTCTTGGT
    GTAGATGAAA ATAGTAGTAA TAGGACTAGA ATCTTTAGAG AACAAGATGA TGGTATGAAT
    TTGACCCAAT GTCATACAGC CTGTATTAAG ACTTGGATTC CTCCATCCAC GGAGGCCAAA
    ATAGGAGAAT TCAAAGGTGA TAAAACAATT TATGGCAATG AGTGTATGGA GTTGACAACT
    AACTATACGA TACAGGTTTT GTCATCAGAA AATAATTTAT CTGAAAGAGA AACTCAAACT
    CAAAATGGTA TGAATGTTAC AACAGTTGAT GGAGCTACAG CTCCAGCTCC AGAGAAGAAA
    ACAGCCCTTA AGGATAAACT AAATGCTGCT TTTCAAGGCT CTTTCCCAAA CCCTGAAAAT
    AAAATTCATA TTATCAAATG TCATCCTATA GAGTCAGAAA CTCATACAGT CACACAGATT
    TCTAGTCAAA GTGCCAGCAC ATTGGCTGTA ACTTCAAAAT CTATATGTTC TAGTCCAGCT
    ATTGAGGGGT ATAAGACAAT TTTTCATTCT AGTAGCAATG ATGCAATGGA ACTGACTAAG
    TGTCTATCAG CTATGGAAGA GGAAAAAAAA TTGCTGAAGG CTGATGATAA GTATTCTAAA
    ATATGTACCA ATCCAGATGC TGGCCCACTC AGAGAGAAAA CTATTTATTT AGAAGAGGAT
    AGCATGGACA TTACCAAAAG TCACACCGTT GCAATAGATA ATAAAATATT TAAACATGAT
    CAAGAAAACA TTAAAAAAGA AATAATAGCC ATACCAATAT TTGAAAAGGA AATGGTGCTC
    CGAAATCTTA TGCCTATGTC AAAAGATGAG AAAAGGGATG TAAATTATAT TTCAGTTCCT
    CAAGTATCTA AGGAAAGCTT ACAACGGAGT CAGACAAACA CTTTATCTGT TTCATTGACT
    GACAAAAAGA TGGAATTCTT GGCAGATGAA GATATGGATC TGACTAAAAG TCACACAACT
    AAGTTAAGTC AAGTTATTCC TACAACTTTT GACTTAGCAT CAAAGAATGT CACCAAATCA
    TACTCTCACA GCAAAAGCCC TTTAAATGAG TGGGAAAGCC TGGATAAACA AGTAGTGCTA
    GGCCAGCATT CTAAACTTCC TTTACCACAG AGGAAAGATA GAGATGATCC TGACTGTTCT
    CATCATAAGA TAATGTATTC AGAGGAGTTA CAAACTATGG ATCTAACCAA GAGCCACACT
    ATTGTAATTG GCTTTGGTCC TTCTGAAGTA CAAGAACATA GTAAAATTAA TTTAGAACAC
    AAAAACAGCC AACTTACAGC AGAAAGCATA CAAACAGCTG TGAACGTTCC TGCAGCAAAC
    TCTAGAGTAG TAACAACTAA TGATATGGAC ATGTTAAAGG ACAGAAGTAC ACATAAGCCA
    GAATTGCTGA AGGAAAAGCA AAACATCAAA ATCTATGGAA GAAAAAGTAT TGGTAGACTG
    AAAATTGATA AAACTATATT ATTTTCAGAG GGTAATGAGG GTGATATGGA TATCACCAAG
    AGTTGCACAG TCAAAATAAA TCATAGATCT TTATTAGATA AGCATGATTC TCATTTAGTA
    TCTTTGGCAG GAACTTCTAA AACCATTTTG CATGCACGTG GTCAGGTTGA AATGGAAATC
    AATAGAAGTC ACACAACTCC TTTAGAATGT AAAATTATCT CGCCCAGTGA TATAACTCCT
    GGTGATCTGG ATAAGACTAT GATGTCTATA GATGATCATG AGGAACTAGA CATGACCAAG
    TCCCATACTG TTTTCATTGA CTACCAAGCA GAGGCAAAAG GTGTACTTCC AGACAGACTT
    GACTTTCAAT TATCCAAAAA GGAAAGCCTA CAGAAGCCGA AAGTGACATC TCTGGCTGAA
    GAGATTTACA TTTCAAAAAA TAGTGAAAGC AACCATCTAC CAGCAAAAGG CAGCCAGCTA
    ACAATATTAG AGGAAGGGTC TAATAGCGGG CTTGGAGAGG AAACTAATGA TGCACAAAAG
    CCTGGATTTC TGAATGAACT TCTGTCAGGC AAAACTCAGA GAAGAAAAAG CCTTAGCCTC
    AAAAACAAGA GTATTACATT TCCAGAGAAT GATAAAAGTT ATAGAGAGAT CCCTCAGAGT
    TCTGCAGTAG AAATAAATAA TGAAACTAGG CTGGAAGACA GAAAGGGCTT CAGTTTTGTG
    CCATTGGCAG GAACTTCCAA ACCAGTTTTG TCTGCATATG GGCCCGAGGA CATGGAGATT
    TCTATAGGTC AAACCACTGC TTCAGAATAT AAAACTGTCC CACCAGAAGA AATAACTACT
    ATACCTATGG ATAAAACTGT AATGTTTGTA GATAATTTCG GTGATCTGGA TGTCACCAGA
    TCCCATACGG TTTTCATCGA TTGTCAAGCC AAAGAAAAGG TACTTGATGA ATATACTAAC
    TTGGGAATAC AGAAAACAAA AACTTTGAGT GGTTCTGAAG GTGATACCCA TATTCAAGAA
    ATCACAAAAA ACCCAGCAGC TCAACACAAA CACCATATGA CAACTGTTAT TCCTTCTAGT
    ACAGTAGTTA GTGATCAAAG TTCAATGAAA ATCAAGTTTC ACAAAGCTGA TAGAGATGAG
    GAGGTCAAAG GAAAGGAAGT GGAAGCCAAT ATGCTGAAAC AAACTAAACC TGAAAGCTGT
    CTGTTAAATA TAACAGATGG AAAAAATGTG GATTTTACAA GCAGTTATAC AGCTGATGTT
    TGTAGATCCA GTGACAAGTA TTCCTCTTTA CCAAATATTT CTTCTTCTGA TAATTCGGGT
    GGTAATACTA TGTCCTTGTG TGATAAAAAT AAGGAGAAAG CTTATAATTG CCAAGTGCCA
    AATGAATTTA CTTATGCAGC TATCTTACCA AGTACATATC ACATGGATTC TAAAAAACTG
    TCTGTCTTTC CACCTTGTCC TTCAAAAGAA GTCACTCAAA CTGAAAGTGC CATAGCGCTG
    CTTAAAGATG AAGATCCGGT AGAAGAGCCT CTGGGGGAGA TGGCTACATT TAACTCAAAG
    CATGTATCTC TTAACCTGGC AAAGGATCAA ACAGAGGCCT TTGTTGATGT TAGTGTTGCT
    TCTCAGCCTC ATCTCTCAGC CCAACAAAGT CCATCAACTC AAAAAGGGCA GGATGTTGCC
    CGTAGAGATG AAGGAATATT AGCTAAAGCT GGGAAGAAGG CTTTACCTTT TCTTCTAGAA
    AATGTCGCTG CATCCACATG GGAAAATGAG TCCAAAATAC CCACTAATGT GGAACACTTT
    GCTGTAACCT ATGAAAAAGA ACTGAGTATA AGTATTCAAA CAGATAAATG TAATACAAAT
    GTACAGAGCC CTAGTAACTC GGCCCTGACA ACACAAGTCA TTCAGACGCA TGCAAATGCT
    GAAGGAGCAC TAGACTTTTT AGTTCCATCT ACTGTTTCAT GTTTTAGTAG TACCAAACCA
    AGTCTGAGTA ATTTGAATAG AAAAACTGAG GAGGTCTTAG ACTTTCAGAC TGTTAATTTA
    TTACCTCCTG CCGAACAGTT ACTTGAAGAA GGAAGTCAGG CACATAGTAT GAGTATAGTA
    CAAGCTACAG AAATATATAG ACTAGGTTCC AGGAATGATA GAGATGAAGA AAGTAAAACT
    TTCTGTAATG AGGCTGAAAC TACATCTGTA CCACTAAAAA CAGCTGTTAA AGACAAAACA
    AGAAGATGTT CATTGGGGAT ATTTTTACCC AAATTACCAA GCAAGAGAAG TTGTAGTATC
    ACTGGTGTTG ATGACCTGGA ACAGATTCTA GCAGATGCAG CTGACTTAAC TCAACTAGAA
    ACTCAGCCAG TCTGTAGTAA AGATCCAGGC ATTGGATCTG TTGCAGCCAA ACTGAATTTG
    AGTCCATCTC AGTTTATAAA TGAGGAAAAT CTCCCTGTAT ATCCTGGTGA GATACTTTCA
    TCAGATTCTG TTAGCCTGGA CATTGAGGAA AGTGTTCTGA TTGACACATC TCAGAGAGAG
    AGTTTACCGT CTGAAAACAA AACCGAAAAC TGCAGGGCCC AAAAAAGAAC CCGGGTTGAA
    GAAAATGATG TTACAAATGA GAAAAAAATC AGAACACATG ACAGTGCACA AGATCAAGAG
    ATTTTTGATA ATCATATGGA AGAGGATATC AATAAAAATG TCAACAGTGT ATTGCTGAAA
    AGCCTGAGCA GGACACCGTC TAGTTGCAGC AGCTCTCTGG ATTCAATCAA GTCTGATGGA
    CTCTCTCTGG ATGTCAGCAC ACAGCGTAAT AGCCAAATGG AATCACAGTT TCTTGGTGAC
    ACTATCTCTG AAGAGAGTTT GAAGGAGAAA CTCAAGGATG GACAAATTAC AATAAAGGAG
    TTCTTCATAC TTCTTCAGGT CCACATCTTA ATACAGAAAC CTAGGCAGAG CACTCTCCCA
    GCCAAATTTA CTATAAACAC ATTACCTACC ACAGAAGATC TGATGTTGAG ACAATATGTT
    TATGGACCCA GGATACAGAT TTATAAAGAA GACTGTGAGC TTCTTCGACA AAAGATTGAC
    GAATTAAAGA TATCTGCATT GAACCAAAAT AAACTATTGG CGGATGTAAA TAGGAACTTG
    TGGGAAAAAG TGAAAGACTA CTCTGATGAA GAGCTGAAGA ACTATGGGAT TTACCTTAAC
    AAAATCAAAT CACGTTATAC CAAGATGACA AAAGTCTTCA ATCACCAAGG AAAAGTGGCT
    CTGTATAACA AATTGGTGCA TTCAGCTGAG AATGAGAAGA ACAAACTTCA GATAAAAATA
    AATGAGATGG ACACTATCCT CAAGAAGATC AATAACTGTC TCGCTGAAGT GGAAACAGAA
    ACTAAGAATT TGGAGAATGA AGAGAAAAAT GATGCCATGG AAGAATGGGA TTCTGAAATG
    AGAGATGCAG AGAAAGAACT GGAACAGCTG AAAACTGAAG AAGAGGAGCT TCAGAGAAAG
    TTCTTAGAAG TAGAGACACA GAAAACGCAG ACCCTTGCTC AGATAGAATT TATAAAAGAA
    CAAACAACTA AAACTGAAGA ATTACTGGAT CAATTGAGTT TGTCTGAGTG GGATGTCATT
    GAGTGGAGTG ATGATCAAGC TGTATTTACC TTTGTCTATG ATTCAATAGA ACTCATCATC
    ACCTTTGGAG AGCCACTAGT TGGTCTCCCT TTTTTGGACA AGGCTTGCAG GAAGATTAAT
    GCACTCAGTT TTCAGTCTCT GTTAGATGAA GATAAAGCTC CTCCCTCCTC CCTTTTAGTT
    CACAAGCTTA TTTTTCAGTA CATTGAGGAA CAGGAATCCT GGAAGAAGAA ATGCACAGCA
    CAGCATCAGG TGCCCCAGAT GCTTCAAGAA CTCTCACTGG TAGTGAACCA TTGTAGGCTT
    CTTGGAGAGG AGATCGAATT TTTAAAGCGA TGGGGGCCAA ATTATAGCCT AATGCATATA
    AATGTGAATA ATACTGAATT GAGGCTTTTA TTCTCCAGTT GTGCAGCATT TGCAAAGTTT
    GAGATAACTT TGTCTCCCTC GGCCCATTAT CCCTTGGTCC CGTTACCGTT CACCATTCAC
    AATCACATTG GGAAGACTGG CCATGATGAA ATTGCTGCAA TTATCTCTAA AGTGCCCTTA
    GAGGAAAACT ACCTGAAGAA TGTAGTCAAG CAAATTTACC AAGATCTACT TAAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.