Asb5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asb5
Entrez Gene ID	76294
Full Name	ankyrin repeat and SOCs box-containing 5
Synonyms	1110018D09Rik
General protein information	Preferred Names ankyrin repeat and SOCs box-containing 5 Names ankyrin repeat and SOCS box protein 5 ASB-5 ankyrin repeat and SOCs box-containing protein 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006509519.3	XP_006509582.1	ankyrin repeat and SOCS box protein 5 isoform X1
XM_006509520.3	XP_006509583.1	ankyrin repeat and SOCS box protein 5 isoform X3
NM_001374795.1	NP_001361724.1	ankyrin repeat and SOCS box protein 5 isoform 3
XM_017313000.1	XP_017168489.1	ankyrin repeat and SOCS box protein 5 isoform X2
NM_001374794.1	NP_001361723.1	ankyrin repeat and SOCS box protein 5 isoform 2
XM_006509520.4	XP_006509583.1	ankyrin repeat and SOCS box protein 5 isoform X3
NM_029569.3	NP_083845.1	ankyrin repeat and SOCS box protein 5 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ASB5	XP_849120.2
Rattus norvegicus (Norway rat)	Asb5	NP_001037712.1
Gallus gallus (chicken)	ASB5	XP_004941139.1
Bos taurus (cattle)	ASB5	NP_001069212.1
Pan troglodytes (chimpanzee)	ASB5	XP_526739.2
Danio rerio (zebrafish)	asb5b	NP_001017753.1
Homo sapiens (human)	ASB5	NP_543150.1
	ASB5	XP_001090118.1
Mus musculus (house mouse)	Asb5	NP_083845.1

Gene Ontology
Bibliography

Related articles in PubMed

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Gineste C, De Winter JM, Kohl C, Witt CC, Giannesini B, Brohm K, Le Fur Y, Gretz N, Vilmen C, Pecchi E, Jubeau M, Cozzone PJ, Stienen GJ, Granzier H, Labeit S, Ottenheijm CA, Bendahan D, Gondin J
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Functional analysis of Asb-1 using genetic modification in mice.
Kile BT, Metcalf D, Mifsud S, DiRago L, Nicola NA, Hilton DJ, Alexander WS
Molecular and cellular biology21(18)6189-97(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Asb5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asb5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07139	NM_029569.3 Latest version!	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu40901	XM_006509519.3 Latest version!	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu40901	NM_001374794.1 Latest version!	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu82591	NM_001374795.1 Latest version!	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu40902	XM_006509520.4 Latest version!	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu82591	XM_017313000.1 Latest version!	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu40902	XM_006509520.3	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asb5 ( NM_029569.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083845.1 Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07139
Clone ID Related Accession (Same CDS sequence)	NM_029569.3
Accession Version	NM_029569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-15
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and SOCS box protein 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102933.6. On Apr 30, 2007 this sequence version replaced NM_029569.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK003770.1, BC108405.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGTGT TAGAAGAAAG CCGGCCATTT GCTCAACAGC TATCTAATGT CTATTTTACG 
ATACTCTCCC TCTTCTGTTT TAAACTTTTT GTGAAGATCA GCCTTGCCAT CCTTAGTCAT 
TTCTACATCG TGAAAGGCAA CCGAAAGGAA GCCGCAAGGA TAGCAGCTGA GTTTTATGGA 
GTAAGCCAAG GACAAGGATC CTGGGCAGAT CGATCACCAC TGCATGAAGC TGCAAGTCAA 
GGTCGTCTTC TTGCTCTGAG GACATTGCTA TCACAAGGAT ATAATGTAAA TGCAGTAACC 
ATAGACCATG TCACTCCACT CCATGAGGCC TGCCTAGGTG ACCACGTGGC CTGTGCAAGG 
ACTCTCCTGG AAGCTGGGGC TAATGCAAAC GCAATCACCA TAGATGGCGT GACTCCCTTG 
TTCAACGCAT GCTCACAAGG CAGCGCAAGT TGTGCAGAAC TCCTTTTGGA ATATGGTGCC 
AAAGCCCAAC TGGAGTCCTG TTTTCCATCT CCAACACATG AGGCTGCCAG TAAAGGTCAC 
CATGAATGTC TCGACATTTT GATAGCCTGG GGCATAGATG TTGACCAAGA CATACCTCAC 
TTGGGAACTC CTCTTTATGT AGCTTGTATG TCACAGCAGT TCCATTGCAT CTGGAAGCTT 
CTTTATGCTG GTGCTGATGT GCACAAAGGC AAGTACTGGG ACACGCCACT GCATGCTGCC 
GCTCAACAAC CCAGTACCGA AATCGTGAAC TTACTGCTAG AATTTGGAGC AGACATCAAT 
GCAAAGAACA CAGATCTCTT GCGGCCTGTC GACTTGGCTA CATCTAACAG TGCTGTAGAA 
AGAATACTTC TTCAACATGA AGCTACCCCA AGCTCTCTTT GCCAACTGTG CCGACTCTGC 
ATCCGAAACT ACATAGGAAG ACAAAGGTTC CATCTTATCC CTCAGCTCCA GTTACCAACA 
TTGCTGCAGA ATTTCTTACA GTATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083845.1
CDS	115..1104
Translation	MSVLEESRPFAQQLSNVYFTILSLFCFKLFVKISLAILSHFYIVKGNRKEAARIAAEFYGVSQGQGSWADRSPLHEAASQGRLLALRTLLSQGYNVNAVTIDHVTPLHEACLGDHVACARTLLEAGANANAITIDGVTPLFNACSQGSASCAELLLEYGAKAQLESCFPSPTHEAASKGHHECLDILIAWGIDVDQDIPHLGTPLYVACMSQQFHCIWKLLYAGADVHKGKYWDTPLHAAAQQPSTEIVNLLLEFGADINAKNTDLLRPVDLATSNSAVERILLQHEATPSSLCQLCRLCIRNYIGRQRFHLIPQLQLPTLLQNFLQYR

Target ORF information:

RefSeq Version	NM_029569.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029569.3

ORF Insert Sequence:

ATGTCGGTGT TAGAAGAAAG CCGGCCATTT GCTCAACAGC TATCTAATGT CTATTTTACG 
ATACTCTCCC TCTTCTGTTT TAAACTTTTT GTGAAGATCA GCCTTGCCAT CCTTAGTCAT 
TTCTACATCG TGAAAGGCAA CCGAAAGGAA GCCGCAAGGA TAGCAGCTGA GTTTTATGGA 
GTAAGCCAAG GACAAGGATC CTGGGCAGAT CGATCACCAC TGCATGAAGC TGCAAGTCAA 
GGTCGTCTTC TTGCTCTGAG GACATTGCTA TCACAAGGAT ATAATGTAAA TGCAGTAACC 
ATAGACCATG TCACTCCACT CCATGAGGCC TGCCTAGGTG ACCACGTGGC CTGTGCAAGG 
ACTCTCCTGG AAGCTGGGGC TAATGCAAAC GCAATCACCA TAGATGGCGT GACTCCCTTG 
TTCAACGCAT GCTCACAAGG CAGCGCAAGT TGTGCAGAAC TCCTTTTGGA ATATGGTGCC 
AAAGCCCAAC TGGAGTCCTG TTTTCCATCT CCAACACATG AGGCTGCCAG TAAAGGTCAC 
CATGAATGTC TCGACATTTT GATAGCCTGG GGCATAGATG TTGACCAAGA CATACCTCAC 
TTGGGAACTC CTCTTTATGT AGCTTGTATG TCACAGCAGT TCCATTGCAT CTGGAAGCTT 
CTTTATGCTG GTGCTGATGT GCACAAAGGC AAGTACTGGG ACACGCCACT GCATGCTGCC 
GCTCAACAAC CCAGTACCGA AATCGTGAAC TTACTGCTAG AATTTGGAGC AGACATCAAT 
GCAAAGAACA CAGATCTCTT GCGGCCTGTC GACTTGGCTA CATCTAACAG TGCTGTAGAA 
AGAATACTTC TTCAACATGA AGCTACCCCA AGCTCTCTTT GCCAACTGTG CCGACTCTGC 
ATCCGAAACT ACATAGGAAG ACAAAGGTTC CATCTTATCC CTCAGCTCCA GTTACCAACA 
TTGCTGCAGA ATTTCTTACA GTATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asb5 ( XM_006509519.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509582.1 Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40901
Clone ID Related Accession (Same CDS sequence)	XM_006509519.3 , NM_001374794.1
Accession Version	XM_006509519.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and SOCS box protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509519.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCAG ACATTGCTGT TGAAGGGAAC GGCATTGAGC AAATGCTCAT GAAAAGAAAG 
CTAGCAAATG TAGAGGAATG CTCTTCTAAG CGAAAAGCTA GCTGGGGTAT TCTGACGAGT 
CAAGGATCCT GGGCAGATCG ATCACCACTG CATGAAGCTG CAAGTCAAGG TCGTCTTCTT 
GCTCTGAGGA CATTGCTATC ACAAGGATAT AATGTAAATG CAGTAACCAT AGACCATGTC 
ACTCCACTCC ATGAGGCCTG CCTAGGTGAC CACGTGGCCT GTGCAAGGAC TCTCCTGGAA 
GCTGGGGCTA ATGCAAACGC AATCACCATA GATGGCGTGA CTCCCTTGTT CAACGCATGC 
TCACAAGGCA GCGCAAGTTG TGCAGAACTC CTTTTGGAAT ATGGTGCCAA AGCCCAACTG 
GAGTCCTGTT TTCCATCTCC AACACATGAG GCTGCCAGTA AAGGTCACCA TGAATGTCTC 
GACATTTTGA TAGCCTGGGG CATAGATGTT GACCAAGACA TACCTCACTT GGGAACTCCT 
CTTTATGTAG CTTGTATGTC ACAGCAGTTC CATTGCATCT GGAAGCTTCT TTATGCTGGT 
GCTGATGTGC ACAAAGGCAA GTACTGGGAC ACGCCACTGC ATGCTGCCGC TCAACAACCC 
AGTACCGAAA TCGTGAACTT ACTGCTAGAA TTTGGAGCAG ACATCAATGC AAAGAACACA 
GATCTCTTGC GGCCTGTCGA CTTGGCTACA TCTAACAGTG CTGTAGAAAG AATACTTCTT 
CAACATGAAG CTACCCCAAG CTCTCTTTGC CAACTGTGCC GACTCTGCAT CCGAAACTAC 
ATAGGAAGAC AAAGGTTCCA TCTTATCCCT CAGCTCCAGT TACCAACATT GCTGCAGAAT 
TTCTTACAGT ATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509582.1
CDS	67..984
Translation	MSADIAVEGNGIEQMLMKRKLANVEECSSKRKASWGILTSQGSWADRSPLHEAASQGRLLALRTLLSQGYNVNAVTIDHVTPLHEACLGDHVACARTLLEAGANANAITIDGVTPLFNACSQGSASCAELLLEYGAKAQLESCFPSPTHEAASKGHHECLDILIAWGIDVDQDIPHLGTPLYVACMSQQFHCIWKLLYAGADVHKGKYWDTPLHAAAQQPSTEIVNLLLEFGADINAKNTDLLRPVDLATSNSAVERILLQHEATPSSLCQLCRLCIRNYIGRQRFHLIPQLQLPTLLQNFLQYR

Target ORF information:

RefSeq Version	XM_006509519.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509519.3

ORF Insert Sequence:

ATGTCTGCAG ACATTGCTGT TGAAGGGAAC GGCATTGAGC AAATGCTCAT GAAAAGAAAG 
CTAGCAAATG TAGAGGAATG CTCTTCTAAG CGAAAAGCTA GCTGGGGTAT TCTGACGAGT 
CAAGGATCCT GGGCAGATCG ATCACCACTG CATGAAGCTG CAAGTCAAGG TCGTCTTCTT 
GCTCTGAGGA CATTGCTATC ACAAGGATAT AATGTAAATG CAGTAACCAT AGACCATGTC 
ACTCCACTCC ATGAGGCCTG CCTAGGTGAC CACGTGGCCT GTGCAAGGAC TCTCCTGGAA 
GCTGGGGCTA ATGCAAACGC AATCACCATA GATGGCGTGA CTCCCTTGTT CAACGCATGC 
TCACAAGGCA GCGCAAGTTG TGCAGAACTC CTTTTGGAAT ATGGTGCCAA AGCCCAACTG 
GAGTCCTGTT TTCCATCTCC AACACATGAG GCTGCCAGTA AAGGTCACCA TGAATGTCTC 
GACATTTTGA TAGCCTGGGG CATAGATGTT GACCAAGACA TACCTCACTT GGGAACTCCT 
CTTTATGTAG CTTGTATGTC ACAGCAGTTC CATTGCATCT GGAAGCTTCT TTATGCTGGT 
GCTGATGTGC ACAAAGGCAA GTACTGGGAC ACGCCACTGC ATGCTGCCGC TCAACAACCC 
AGTACCGAAA TCGTGAACTT ACTGCTAGAA TTTGGAGCAG ACATCAATGC AAAGAACACA 
GATCTCTTGC GGCCTGTCGA CTTGGCTACA TCTAACAGTG CTGTAGAAAG AATACTTCTT 
CAACATGAAG CTACCCCAAG CTCTCTTTGC CAACTGTGCC GACTCTGCAT CCGAAACTAC 
ATAGGAAGAC AAAGGTTCCA TCTTATCCCT CAGCTCCAGT TACCAACATT GCTGCAGAAT 
TTCTTACAGT ATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asb5 ( XM_006509520.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509583.1 Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu40902
Clone ID Related Accession (Same CDS sequence)	XM_006509520.3 , XM_006509520.4
Accession Version	XM_006509520.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and SOCS box protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509520.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTCT GTAAGGATGA AAACTTTCTG CTGAGCGGAT CCTGGGCAGA TCGATCACCA 
CTGCATGAAG CTGCAAGTCA AGGTCGTCTT CTTGCTCTGA GGACATTGCT ATCACAAGGA 
TATAATGTAA ATGCAGTAAC CATAGACCAT GTCACTCCAC TCCATGAGGC CTGCCTAGGT 
GACCACGTGG CCTGTGCAAG GACTCTCCTG GAAGCTGGGG CTAATGCAAA CGCAATCACC 
ATAGATGGCG TGACTCCCTT GTTCAACGCA TGCTCACAAG GCAGCGCAAG TTGTGCAGAA 
CTCCTTTTGG AATATGGTGC CAAAGCCCAA CTGGAGTCCT GTTTTCCATC TCCAACACAT 
GAGGCTGCCA GTAAAGGTCA CCATGAATGT CTCGACATTT TGATAGCCTG GGGCATAGAT 
GTTGACCAAG ACATACCTCA CTTGGGAACT CCTCTTTATG TAGCTTGTAT GTCACAGCAG 
TTCCATTGCA TCTGGAAGCT TCTTTATGCT GGTGCTGATG TGCACAAAGG CAAGTACTGG 
GACACGCCAC TGCATGCTGC CGCTCAACAA CCCAGTACCG AAATCGTGAA CTTACTGCTA 
GAATTTGGAG CAGACATCAA TGCAAAGAAC ACAGATCTCT TGCGGCCTGT CGACTTGGCT 
ACATCTAACA GTGCTGTAGA AAGAATACTT CTTCAACATG AAGCTACCCC AAGCTCTCTT 
TGCCAACTGT GCCGACTCTG CATCCGAAAC TACATAGGAA GACAAAGGTT CCATCTTATC 
CCTCAGCTCC AGTTACCAAC ATTGCTGCAG AATTTCTTAC AGTATCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509583.1
CDS	158..988
Translation	MRLCKDENFLLSGSWADRSPLHEAASQGRLLALRTLLSQGYNVNAVTIDHVTPLHEACLGDHVACARTLLEAGANANAITIDGVTPLFNACSQGSASCAELLLEYGAKAQLESCFPSPTHEAASKGHHECLDILIAWGIDVDQDIPHLGTPLYVACMSQQFHCIWKLLYAGADVHKGKYWDTPLHAAAQQPSTEIVNLLLEFGADINAKNTDLLRPVDLATSNSAVERILLQHEATPSSLCQLCRLCIRNYIGRQRFHLIPQLQLPTLLQNFLQYR

Target ORF information:

RefSeq Version	XM_006509520.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509520.3

ORF Insert Sequence:

ATGCGGCTCT GTAAGGATGA AAACTTTCTG CTGAGCGGAT CCTGGGCAGA TCGATCACCA 
CTGCATGAAG CTGCAAGTCA AGGTCGTCTT CTTGCTCTGA GGACATTGCT ATCACAAGGA 
TATAATGTAA ATGCAGTAAC CATAGACCAT GTCACTCCAC TCCATGAGGC CTGCCTAGGT 
GACCACGTGG CCTGTGCAAG GACTCTCCTG GAAGCTGGGG CTAATGCAAA CGCAATCACC 
ATAGATGGCG TGACTCCCTT GTTCAACGCA TGCTCACAAG GCAGCGCAAG TTGTGCAGAA 
CTCCTTTTGG AATATGGTGC CAAAGCCCAA CTGGAGTCCT GTTTTCCATC TCCAACACAT 
GAGGCTGCCA GTAAAGGTCA CCATGAATGT CTCGACATTT TGATAGCCTG GGGCATAGAT 
GTTGACCAAG ACATACCTCA CTTGGGAACT CCTCTTTATG TAGCTTGTAT GTCACAGCAG 
TTCCATTGCA TCTGGAAGCT TCTTTATGCT GGTGCTGATG TGCACAAAGG CAAGTACTGG 
GACACGCCAC TGCATGCTGC CGCTCAACAA CCCAGTACCG AAATCGTGAA CTTACTGCTA 
GAATTTGGAG CAGACATCAA TGCAAAGAAC ACAGATCTCT TGCGGCCTGT CGACTTGGCT 
ACATCTAACA GTGCTGTAGA AAGAATACTT CTTCAACATG AAGCTACCCC AAGCTCTCTT 
TGCCAACTGT GCCGACTCTG CATCCGAAAC TACATAGGAA GACAAAGGTT CCATCTTATC 
CCTCAGCTCC AGTTACCAAC ATTGCTGCAG AATTTCTTAC AGTATCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asb5 ( NM_001374794.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001361723.1 Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu40901
Clone ID Related Accession (Same CDS sequence)	XM_006509519.3 , NM_001374794.1
Accession Version	NM_001374794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and SOCS box protein 5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102933.6. On Oct 16, 2019 this sequence version replaced XM_006509519.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5189679.184606.1, SRR7345562.2947165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCAG ACATTGCTGT TGAAGGGAAC GGCATTGAGC AAATGCTCAT GAAAAGAAAG 
CTAGCAAATG TAGAGGAATG CTCTTCTAAG CGAAAAGCTA GCTGGGGTAT TCTGACGAGT 
CAAGGATCCT GGGCAGATCG ATCACCACTG CATGAAGCTG CAAGTCAAGG TCGTCTTCTT 
GCTCTGAGGA CATTGCTATC ACAAGGATAT AATGTAAATG CAGTAACCAT AGACCATGTC 
ACTCCACTCC ATGAGGCCTG CCTAGGTGAC CACGTGGCCT GTGCAAGGAC TCTCCTGGAA 
GCTGGGGCTA ATGCAAACGC AATCACCATA GATGGCGTGA CTCCCTTGTT CAACGCATGC 
TCACAAGGCA GCGCAAGTTG TGCAGAACTC CTTTTGGAAT ATGGTGCCAA AGCCCAACTG 
GAGTCCTGTT TTCCATCTCC AACACATGAG GCTGCCAGTA AAGGTCACCA TGAATGTCTC 
GACATTTTGA TAGCCTGGGG CATAGATGTT GACCAAGACA TACCTCACTT GGGAACTCCT 
CTTTATGTAG CTTGTATGTC ACAGCAGTTC CATTGCATCT GGAAGCTTCT TTATGCTGGT 
GCTGATGTGC ACAAAGGCAA GTACTGGGAC ACGCCACTGC ATGCTGCCGC TCAACAACCC 
AGTACCGAAA TCGTGAACTT ACTGCTAGAA TTTGGAGCAG ACATCAATGC AAAGAACACA 
GATCTCTTGC GGCCTGTCGA CTTGGCTACA TCTAACAGTG CTGTAGAAAG AATACTTCTT 
CAACATGAAG CTACCCCAAG CTCTCTTTGC CAACTGTGCC GACTCTGCAT CCGAAACTAC 
ATAGGAAGAC AAAGGTTCCA TCTTATCCCT CAGCTCCAGT TACCAACATT GCTGCAGAAT 
TTCTTACAGT ATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361723.1
CDS	174..1091
Translation	MSADIAVEGNGIEQMLMKRKLANVEECSSKRKASWGILTSQGSWADRSPLHEAASQGRLLALRTLLSQGYNVNAVTIDHVTPLHEACLGDHVACARTLLEAGANANAITIDGVTPLFNACSQGSASCAELLLEYGAKAQLESCFPSPTHEAASKGHHECLDILIAWGIDVDQDIPHLGTPLYVACMSQQFHCIWKLLYAGADVHKGKYWDTPLHAAAQQPSTEIVNLLLEFGADINAKNTDLLRPVDLATSNSAVERILLQHEATPSSLCQLCRLCIRNYIGRQRFHLIPQLQLPTLLQNFLQYR

Target ORF information:

RefSeq Version	NM_001374794.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374794.1

ORF Insert Sequence:

ATGTCTGCAG ACATTGCTGT TGAAGGGAAC GGCATTGAGC AAATGCTCAT GAAAAGAAAG 
CTAGCAAATG TAGAGGAATG CTCTTCTAAG CGAAAAGCTA GCTGGGGTAT TCTGACGAGT 
CAAGGATCCT GGGCAGATCG ATCACCACTG CATGAAGCTG CAAGTCAAGG TCGTCTTCTT 
GCTCTGAGGA CATTGCTATC ACAAGGATAT AATGTAAATG CAGTAACCAT AGACCATGTC 
ACTCCACTCC ATGAGGCCTG CCTAGGTGAC CACGTGGCCT GTGCAAGGAC TCTCCTGGAA 
GCTGGGGCTA ATGCAAACGC AATCACCATA GATGGCGTGA CTCCCTTGTT CAACGCATGC 
TCACAAGGCA GCGCAAGTTG TGCAGAACTC CTTTTGGAAT ATGGTGCCAA AGCCCAACTG 
GAGTCCTGTT TTCCATCTCC AACACATGAG GCTGCCAGTA AAGGTCACCA TGAATGTCTC 
GACATTTTGA TAGCCTGGGG CATAGATGTT GACCAAGACA TACCTCACTT GGGAACTCCT 
CTTTATGTAG CTTGTATGTC ACAGCAGTTC CATTGCATCT GGAAGCTTCT TTATGCTGGT 
GCTGATGTGC ACAAAGGCAA GTACTGGGAC ACGCCACTGC ATGCTGCCGC TCAACAACCC 
AGTACCGAAA TCGTGAACTT ACTGCTAGAA TTTGGAGCAG ACATCAATGC AAAGAACACA 
GATCTCTTGC GGCCTGTCGA CTTGGCTACA TCTAACAGTG CTGTAGAAAG AATACTTCTT 
CAACATGAAG CTACCCCAAG CTCTCTTTGC CAACTGTGCC GACTCTGCAT CCGAAACTAC 
ATAGGAAGAC AAAGGTTCCA TCTTATCCCT CAGCTCCAGT TACCAACATT GCTGCAGAAT 
TTCTTACAGT ATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asb5 ( NM_001374795.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001361724.1 Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant 3, mRNA.

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CloneID	OMu82591
Clone ID Related Accession (Same CDS sequence)	NM_001374795.1 , XM_017313000.1
Accession Version	NM_001374795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and SOCS box protein 5 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102933.6. On Oct 17, 2019 this sequence version replaced XM_017313000.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: ERR2680379.3535.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGAGTCGGG TTTACCTCAG CAGTGGCTGG CTTGAAGTGC CATGGGGAGA TGGTGACTTA 
GGATCCTGGG CAGATCGATC ACCACTGCAT GAAGCTGCAA GTCAAGGTCG TCTTCTTGCT 
CTGAGGACAT TGCTATCACA AGGATATAAT GTAAATGCAG TAACCATAGA CCATGTCACT 
CCACTCCATG AGGCCTGCCT AGGTGACCAC GTGGCCTGTG CAAGGACTCT CCTGGAAGCT 
GGGGCTAATG CAAACGCAAT CACCATAGAT GGCGTGACTC CCTTGTTCAA CGCATGCTCA 
CAAGGCAGCG CAAGTTGTGC AGAACTCCTT TTGGAATATG GTGCCAAAGC CCAACTGGAG 
TCCTGTTTTC CATCTCCAAC ACATGAGGCT GCCAGTAAAG GTCACCATGA ATGTCTCGAC 
ATTTTGATAG CCTGGGGCAT AGATGTTGAC CAAGACATAC CTCACTTGGG AACTCCTCTT 
TATGTAGCTT GTATGTCACA GCAGTTCCAT TGCATCTGGA AGCTTCTTTA TGCTGGTGCT 
GATGTGCACA AAGGCAAGTA CTGGGACACG CCACTGCATG CTGCCGCTCA ACAACCCAGT 
ACCGAAATCG TGAACTTACT GCTAGAATTT GGAGCAGACA TCAATGCAAA GAACACAGAT 
CTCTTGCGGC CTGTCGACTT GGCTACATCT AACAGTGCTG TAGAAAGAAT ACTTCTTCAA 
CATGAAGCTA CCCCAAGCTC TCTTTGCCAA CTGTGCCGAC TCTGCATCCG AAACTACATA 
GGAAGACAAA GGTTCCATCT TATCCCTCAG CTCCAGTTAC CAACATTGCT GCAGAATTTC 
TTACAGTATC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361724.1
CDS	16..870
Translation	MSRVYLSSGWLEVPWGDGDLGSWADRSPLHEAASQGRLLALRTLLSQGYNVNAVTIDHVTPLHEACLGDHVACARTLLEAGANANAITIDGVTPLFNACSQGSASCAELLLEYGAKAQLESCFPSPTHEAASKGHHECLDILIAWGIDVDQDIPHLGTPLYVACMSQQFHCIWKLLYAGADVHKGKYWDTPLHAAAQQPSTEIVNLLLEFGADINAKNTDLLRPVDLATSNSAVERILLQHEATPSSLCQLCRLCIRNYIGRQRFHLIPQLQLPTLLQNFLQYR

Target ORF information:

RefSeq Version	NM_001374795.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374795.1

ORF Insert Sequence:

ATGAGTCGGG TTTACCTCAG CAGTGGCTGG CTTGAAGTGC CATGGGGAGA TGGTGACTTA 
GGATCCTGGG CAGATCGATC ACCACTGCAT GAAGCTGCAA GTCAAGGTCG TCTTCTTGCT 
CTGAGGACAT TGCTATCACA AGGATATAAT GTAAATGCAG TAACCATAGA CCATGTCACT 
CCACTCCATG AGGCCTGCCT AGGTGACCAC GTGGCCTGTG CAAGGACTCT CCTGGAAGCT 
GGGGCTAATG CAAACGCAAT CACCATAGAT GGCGTGACTC CCTTGTTCAA CGCATGCTCA 
CAAGGCAGCG CAAGTTGTGC AGAACTCCTT TTGGAATATG GTGCCAAAGC CCAACTGGAG 
TCCTGTTTTC CATCTCCAAC ACATGAGGCT GCCAGTAAAG GTCACCATGA ATGTCTCGAC 
ATTTTGATAG CCTGGGGCAT AGATGTTGAC CAAGACATAC CTCACTTGGG AACTCCTCTT 
TATGTAGCTT GTATGTCACA GCAGTTCCAT TGCATCTGGA AGCTTCTTTA TGCTGGTGCT 
GATGTGCACA AAGGCAAGTA CTGGGACACG CCACTGCATG CTGCCGCTCA ACAACCCAGT 
ACCGAAATCG TGAACTTACT GCTAGAATTT GGAGCAGACA TCAATGCAAA GAACACAGAT 
CTCTTGCGGC CTGTCGACTT GGCTACATCT AACAGTGCTG TAGAAAGAAT ACTTCTTCAA 
CATGAAGCTA CCCCAAGCTC TCTTTGCCAA CTGTGCCGAC TCTGCATCCG AAACTACATA 
GGAAGACAAA GGTTCCATCT TATCCCTCAG CTCCAGTTAC CAACATTGCT GCAGAATTTC 
TTACAGTATC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asb5 ( XM_006509520.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509583.1 Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X3, mRNA.

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CloneID	OMu40902
Clone ID Related Accession (Same CDS sequence)	XM_006509520.3 , XM_006509520.4
Accession Version	XM_006509520.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and SOCS box protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509520.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTCT GTAAGGATGA AAACTTTCTG CTGAGCGGAT CCTGGGCAGA TCGATCACCA 
CTGCATGAAG CTGCAAGTCA AGGTCGTCTT CTTGCTCTGA GGACATTGCT ATCACAAGGA 
TATAATGTAA ATGCAGTAAC CATAGACCAT GTCACTCCAC TCCATGAGGC CTGCCTAGGT 
GACCACGTGG CCTGTGCAAG GACTCTCCTG GAAGCTGGGG CTAATGCAAA CGCAATCACC 
ATAGATGGCG TGACTCCCTT GTTCAACGCA TGCTCACAAG GCAGCGCAAG TTGTGCAGAA 
CTCCTTTTGG AATATGGTGC CAAAGCCCAA CTGGAGTCCT GTTTTCCATC TCCAACACAT 
GAGGCTGCCA GTAAAGGTCA CCATGAATGT CTCGACATTT TGATAGCCTG GGGCATAGAT 
GTTGACCAAG ACATACCTCA CTTGGGAACT CCTCTTTATG TAGCTTGTAT GTCACAGCAG 
TTCCATTGCA TCTGGAAGCT TCTTTATGCT GGTGCTGATG TGCACAAAGG CAAGTACTGG 
GACACGCCAC TGCATGCTGC CGCTCAACAA CCCAGTACCG AAATCGTGAA CTTACTGCTA 
GAATTTGGAG CAGACATCAA TGCAAAGAAC ACAGATCTCT TGCGGCCTGT CGACTTGGCT 
ACATCTAACA GTGCTGTAGA AAGAATACTT CTTCAACATG AAGCTACCCC AAGCTCTCTT 
TGCCAACTGT GCCGACTCTG CATCCGAAAC TACATAGGAA GACAAAGGTT CCATCTTATC 
CCTCAGCTCC AGTTACCAAC ATTGCTGCAG AATTTCTTAC AGTATCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509583.1
CDS	197..1027
Translation	MRLCKDENFLLSGSWADRSPLHEAASQGRLLALRTLLSQGYNVNAVTIDHVTPLHEACLGDHVACARTLLEAGANANAITIDGVTPLFNACSQGSASCAELLLEYGAKAQLESCFPSPTHEAASKGHHECLDILIAWGIDVDQDIPHLGTPLYVACMSQQFHCIWKLLYAGADVHKGKYWDTPLHAAAQQPSTEIVNLLLEFGADINAKNTDLLRPVDLATSNSAVERILLQHEATPSSLCQLCRLCIRNYIGRQRFHLIPQLQLPTLLQNFLQYR

Target ORF information:

RefSeq Version	XM_006509520.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509520.4

ORF Insert Sequence:

ATGCGGCTCT GTAAGGATGA AAACTTTCTG CTGAGCGGAT CCTGGGCAGA TCGATCACCA 
CTGCATGAAG CTGCAAGTCA AGGTCGTCTT CTTGCTCTGA GGACATTGCT ATCACAAGGA 
TATAATGTAA ATGCAGTAAC CATAGACCAT GTCACTCCAC TCCATGAGGC CTGCCTAGGT 
GACCACGTGG CCTGTGCAAG GACTCTCCTG GAAGCTGGGG CTAATGCAAA CGCAATCACC 
ATAGATGGCG TGACTCCCTT GTTCAACGCA TGCTCACAAG GCAGCGCAAG TTGTGCAGAA 
CTCCTTTTGG AATATGGTGC CAAAGCCCAA CTGGAGTCCT GTTTTCCATC TCCAACACAT 
GAGGCTGCCA GTAAAGGTCA CCATGAATGT CTCGACATTT TGATAGCCTG GGGCATAGAT 
GTTGACCAAG ACATACCTCA CTTGGGAACT CCTCTTTATG TAGCTTGTAT GTCACAGCAG 
TTCCATTGCA TCTGGAAGCT TCTTTATGCT GGTGCTGATG TGCACAAAGG CAAGTACTGG 
GACACGCCAC TGCATGCTGC CGCTCAACAA CCCAGTACCG AAATCGTGAA CTTACTGCTA 
GAATTTGGAG CAGACATCAA TGCAAAGAAC ACAGATCTCT TGCGGCCTGT CGACTTGGCT 
ACATCTAACA GTGCTGTAGA AAGAATACTT CTTCAACATG AAGCTACCCC AAGCTCTCTT 
TGCCAACTGT GCCGACTCTG CATCCGAAAC TACATAGGAA GACAAAGGTT CCATCTTATC 
CCTCAGCTCC AGTTACCAAC ATTGCTGCAG AATTTCTTAC AGTATCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asb5 ( XM_017313000.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168489.1 Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu82591
Clone ID Related Accession (Same CDS sequence)	NM_001374795.1 , XM_017313000.1
Accession Version	XM_017313000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and SOCS box protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGGG TTTACCTCAG CAGTGGCTGG CTTGAAGTGC CATGGGGAGA TGGTGACTTA 
GGATCCTGGG CAGATCGATC ACCACTGCAT GAAGCTGCAA GTCAAGGTCG TCTTCTTGCT 
CTGAGGACAT TGCTATCACA AGGATATAAT GTAAATGCAG TAACCATAGA CCATGTCACT 
CCACTCCATG AGGCCTGCCT AGGTGACCAC GTGGCCTGTG CAAGGACTCT CCTGGAAGCT 
GGGGCTAATG CAAACGCAAT CACCATAGAT GGCGTGACTC CCTTGTTCAA CGCATGCTCA 
CAAGGCAGCG CAAGTTGTGC AGAACTCCTT TTGGAATATG GTGCCAAAGC CCAACTGGAG 
TCCTGTTTTC CATCTCCAAC ACATGAGGCT GCCAGTAAAG GTCACCATGA ATGTCTCGAC 
ATTTTGATAG CCTGGGGCAT AGATGTTGAC CAAGACATAC CTCACTTGGG AACTCCTCTT 
TATGTAGCTT GTATGTCACA GCAGTTCCAT TGCATCTGGA AGCTTCTTTA TGCTGGTGCT 
GATGTGCACA AAGGCAAGTA CTGGGACACG CCACTGCATG CTGCCGCTCA ACAACCCAGT 
ACCGAAATCG TGAACTTACT GCTAGAATTT GGAGCAGACA TCAATGCAAA GAACACAGAT 
CTCTTGCGGC CTGTCGACTT GGCTACATCT AACAGTGCTG TAGAAAGAAT ACTTCTTCAA 
CATGAAGCTA CCCCAAGCTC TCTTTGCCAA CTGTGCCGAC TCTGCATCCG AAACTACATA 
GGAAGACAAA GGTTCCATCT TATCCCTCAG CTCCAGTTAC CAACATTGCT GCAGAATTTC 
TTACAGTATC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168489.1
CDS	16..870
Translation	MSRVYLSSGWLEVPWGDGDLGSWADRSPLHEAASQGRLLALRTLLSQGYNVNAVTIDHVTPLHEACLGDHVACARTLLEAGANANAITIDGVTPLFNACSQGSASCAELLLEYGAKAQLESCFPSPTHEAASKGHHECLDILIAWGIDVDQDIPHLGTPLYVACMSQQFHCIWKLLYAGADVHKGKYWDTPLHAAAQQPSTEIVNLLLEFGADINAKNTDLLRPVDLATSNSAVERILLQHEATPSSLCQLCRLCIRNYIGRQRFHLIPQLQLPTLLQNFLQYR

Target ORF information:

RefSeq Version	XM_017313000.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin repeat and SOCs box-containing 5 (Asb5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313000.1

ORF Insert Sequence:

ATGAGTCGGG TTTACCTCAG CAGTGGCTGG CTTGAAGTGC CATGGGGAGA TGGTGACTTA 
GGATCCTGGG CAGATCGATC ACCACTGCAT GAAGCTGCAA GTCAAGGTCG TCTTCTTGCT 
CTGAGGACAT TGCTATCACA AGGATATAAT GTAAATGCAG TAACCATAGA CCATGTCACT 
CCACTCCATG AGGCCTGCCT AGGTGACCAC GTGGCCTGTG CAAGGACTCT CCTGGAAGCT 
GGGGCTAATG CAAACGCAAT CACCATAGAT GGCGTGACTC CCTTGTTCAA CGCATGCTCA 
CAAGGCAGCG CAAGTTGTGC AGAACTCCTT TTGGAATATG GTGCCAAAGC CCAACTGGAG 
TCCTGTTTTC CATCTCCAAC ACATGAGGCT GCCAGTAAAG GTCACCATGA ATGTCTCGAC 
ATTTTGATAG CCTGGGGCAT AGATGTTGAC CAAGACATAC CTCACTTGGG AACTCCTCTT 
TATGTAGCTT GTATGTCACA GCAGTTCCAT TGCATCTGGA AGCTTCTTTA TGCTGGTGCT 
GATGTGCACA AAGGCAAGTA CTGGGACACG CCACTGCATG CTGCCGCTCA ACAACCCAGT 
ACCGAAATCG TGAACTTACT GCTAGAATTT GGAGCAGACA TCAATGCAAA GAACACAGAT 
CTCTTGCGGC CTGTCGACTT GGCTACATCT AACAGTGCTG TAGAAAGAAT ACTTCTTCAA 
CATGAAGCTA CCCCAAGCTC TCTTTGCCAA CTGTGCCGAC TCTGCATCCG AAACTACATA 
GGAAGACAAA GGTTCCATCT TATCCCTCAG CTCCAGTTAC CAACATTGCT GCAGAATTTC 
TTACAGTATC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)
Gineste C,De Winter JM,Kohl C,Witt CC,Giannesini B,Brohm K,Le Fur Y,Gretz N,Vilmen C,Pecchi E,Jubeau M,Cozzone PJ,Stienen GJ,Granzier H,Labeit S,Ottenheijm CA,Bendahan D,Gondin J

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Functional analysis of Asb-1 using genetic modification in mice.
Molecular and cellular biology21(18)6189-97(2001 Sep)
Kile BT,Metcalf D,Mifsud S,DiRago L,Nicola NA,Hilton DJ,Alexander WS

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Asb5 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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