Gstk1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gstk1
Entrez Gene ID	76263
Full Name	glutathione S-transferase kappa 1
Synonyms	0610025I19Rik,AW260476,DsbA-L
General protein information	Preferred Names glutathione S-transferase kappa 1 Names glutathione S-transferase kappa 1 GST 13-13 GST class-kappa GSTK1-1 glutathione S-transferase class kappa glutathione S-transferase subunit 13 mGSTK1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 B2.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_029555.2	NP_083831.1	glutathione S-transferase kappa 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GSTK1	XP_005629585.1
Danio rerio (zebrafish)	si:ch211-198d13.2	XP_001922199.1
Rattus norvegicus (Norway rat)	Gstk1	NP_852036.1
Danio rerio (zebrafish)	gstk1	NP_001002560.1
Danio rerio (zebrafish)	LOC100537097	XP_003200260.2
Danio rerio (zebrafish)	LOC101885925	XP_005158439.1
Pan troglodytes (chimpanzee)	GSTK1	XP_519446.2
	GSTK1	XP_001087577.1
Bos taurus (cattle)	GSTK1	NP_001073084.1
Caenorhabditis elegans (roundworm)	gstk-1	NP_496082.1
Xenopus tropicalis (tropical clawed frog)	LOC101730645	XP_004915860.1
Homo sapiens (human)	GSTK1	NP_001137151.1
Gallus gallus (chicken)	GSTK1	NP_001185578.1
Danio rerio (zebrafish)	zgc:136861	NP_001035390.1
Xenopus tropicalis (tropical clawed frog)	gstk1	NP_001011244.1
Mus musculus (house mouse)	Gstk1	NP_083831.1

Gene Ontology
Bibliography

Related articles in PubMed

DsbA-L prevents obesity-induced inflammation and insulin resistance by suppressing the mtDNA release-activated cGAS-cGAMP-STING pathway.
Bai J, Cervantes C, Liu J, He S, Zhou H, Zhang B, Cai H, Yin D, Hu D, Li Z, Chen H, Gao X, Wang F, O'Connor JC, Xu Y, Liu M, Dong LQ, Liu F
Proceedings of the National Academy of Sciences of the United States of America114(46)12196-12201(2017 Nov)

Hepatic DsbA-L protects mice from diet-induced hepatosteatosis and insulin resistance.
Chen H, Bai J, Dong F, Fang H, Zhang Y, Meng W, Liu B, Luo Y, Liu M, Bai Y, Abdul-Ghani MA, Li R, Wu J, Zeng R, Zhou Z, Dong LQ, Liu F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(6)2314-2326(2017 06)

Endoplasmic reticulum (ER) localization is critical for DsbA-L protein to suppress ER stress and adiponectin down-regulation in adipocytes.
Liu M, Chen H, Wei L, Hu D, Dong K, Jia W, Dong LQ, Liu F
The Journal of biological chemistry290(16)10143-8(2015 Apr)

Negative regulation of DsbA-L gene expression by the transcription factor Sp1.
Fang Q, Yang W, Li H, Hu W, Chen L, Jiang S, Dong K, Song Q, Wang C, Chen S, Liu F, Jia W
Diabetes63(12)4165-71(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results identify DsbA-L as a critical regulator of mitochondrial function, and its down-regulation in the liver may contribute to obesity-induced hepatosteatosis and whole body insulin resistance.
Title: Hepatic DsbA-L protects mice from diet-induced hepatosteatosis and insulin resistance.

DsbA-L is localized in both the mitochondria and the endoplasmic reticulum (ER) in adipocytes; its ER localization plays a critical role in suppressing ER stress and promoting adiponectin biosynthesis and secretion.
Title: Endoplasmic reticulum (ER) localization is critical for DsbA-L protein to suppress ER stress and adiponectin down-regulation in adipocytes.

Results identify Sp1 as an inhibitor of DsbA-L gene transcription, and the Sp1-mediated inhibition of DsbA-L gene expression may provide a mechanism underlying obesity-induced adiponectin downregulation and insulin resistance.
Title: Negative regulation of DsbA-L gene expression by the transcription factor Sp1.

Data suggest DsbA-L plays a role in promoting adiponectin multimerization and function (sufficient to suppress obesity-induced insulin resistance and liver damage).
Title: Fat-specific DsbA-L overexpression promotes adiponectin multimerization and protects mice from diet-induced obesity and insulin resistance.

significant increases in the levels of total glutathione in Protandim (an antioxidant that consists of ashwagandha, bacopa extract, green tea extract, silymarin, and curcumin; Chinese medicine)-treated cells
Title: Synergistic induction of heme oxygenase-1 by the components of the antioxidant supplement Protandim.

The following Gstk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gstk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15808	NM_029555.2 Latest version!	Mus musculus glutathione S-transferase kappa 1 (Gstk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gstk1 ( NM_029555.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083831.1 Mus musculus glutathione S-transferase kappa 1 (Gstk1), mRNA.

Price & Availability↑

CloneID	OMu15808
Clone ID Related Accession (Same CDS sequence)	NM_029555.2
Accession Version	NM_029555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	glutathione S-transferase kappa 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC155654.16. On Nov 17, 2006 this sequence version replaced NM_029555.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK002661.1, DV051990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGGGCCGG CGCCGCGCAT CCTGGAACTC TTCTACGACG TGCTGTCCCC GTACTCCTGG 
CTGGGCTTTG AGGTCCTATG CAGATACCAA CACCTCTGGA ATATAAAGCT GCAGTTGCGG 
CCCACTTTAA TCGCTGGGAT CATGAAAGAC AGCGGAAACC AACCACCTGC TATGGTTCCC 
CGAAAAGGCC AGTACATCTT CAAAGAGATT CCTCTCCTGA AGCAGTTCTT CCAGGTTCCC 
CTCAACATAC CCAAGGATTT CTTTGGTGAG ACTGTGAAGA AAGGAAGTAT AAATGCCATG 
CGCTTCCTCA CCACTGTAAG CATGGAGCAA CCAGAGATGC TGGAGAAGGT GTCCAGAGAG 
ATATGGATGC GTGTATGGTC TCGAGATGAA GATATCACGG AGTATCAGAG CATTCTGGCT 
GCAGCAGTGA AGGCTGGAAT GTCCACAGCG CAAGCCCAAC ACTTTCTGGA GAAGATCTCC 
ACACAACAGG TGAAGAACAA GCTCATTGAG AACACGGATG CAGCCTGCAA ATATGGGGCC 
TTTGGGCTAC CCACCACTGT TGCCCATGTG GATGGTAAAA CCTACATGTT ATTTGGGTCT 
GACCGCTTGG AGTTGCTAGC TTACCTGCTA GGAGAGAAGT GGATGGGCCC TGTACCCCCA 
ACTGCGAATG CCAGACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083831.1
CDS	88..768
Translation	MGPAPRILELFYDVLSPYSWLGFEVLCRYQHLWNIKLQLRPTLIAGIMKDSGNQPPAMVPRKGQYIFKEIPLLKQFFQVPLNIPKDFFGETVKKGSINAMRFLTTVSMEQPEMLEKVSREIWMRVWSRDEDITEYQSILAAAVKAGMSTAQAQHFLEKISTQQVKNKLIENTDAACKYGAFGLPTTVAHVDGKTYMLFGSDRLELLAYLLGEKWMGPVPPTANARL

Target ORF information:

RefSeq Version	NM_029555.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutathione S-transferase kappa 1 (Gstk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029555.2

ORF Insert Sequence:

ATGGGGCCGG CGCCGCGCAT CCTGGAACTC TTCTACGACG TGCTGTCCCC GTACTCCTGG 
CTGGGCTTTG AGGTCCTATG CAGATACCAA CACCTCTGGA ATATAAAGCT GCAGTTGCGG 
CCCACTTTAA TCGCTGGGAT CATGAAAGAC AGCGGAAACC AACCACCTGC TATGGTTCCC 
CGAAAAGGCC AGTACATCTT CAAAGAGATT CCTCTCCTGA AGCAGTTCTT CCAGGTTCCC 
CTCAACATAC CCAAGGATTT CTTTGGTGAG ACTGTGAAGA AAGGAAGTAT AAATGCCATG 
CGCTTCCTCA CCACTGTAAG CATGGAGCAA CCAGAGATGC TGGAGAAGGT GTCCAGAGAG 
ATATGGATGC GTGTATGGTC TCGAGATGAA GATATCACGG AGTATCAGAG CATTCTGGCT 
GCAGCAGTGA AGGCTGGAAT GTCCACAGCG CAAGCCCAAC ACTTTCTGGA GAAGATCTCC 
ACACAACAGG TGAAGAACAA GCTCATTGAG AACACGGATG CAGCCTGCAA ATATGGGGCC 
TTTGGGCTAC CCACCACTGT TGCCCATGTG GATGGTAAAA CCTACATGTT ATTTGGGTCT 
GACCGCTTGG AGTTGCTAGC TTACCTGCTA GGAGAGAAGT GGATGGGCCC TGTACCCCCA 
ACTGCGAATG CCAGACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DsbA-L prevents obesity-induced inflammation and insulin resistance by suppressing the mtDNA release-activated cGAS-cGAMP-STING pathway.
Proceedings of the National Academy of Sciences of the United States of America114(46)12196-12201(2017 Nov)
Bai J,Cervantes C,Liu J,He S,Zhou H,Zhang B,Cai H,Yin D,Hu D,Li Z,Chen H,Gao X,Wang F,O'Connor JC,Xu Y,Liu M,Dong LQ,Liu F

Hepatic DsbA-L protects mice from diet-induced hepatosteatosis and insulin resistance.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(6)2314-2326(2017 06)
Chen H,Bai J,Dong F,Fang H,Zhang Y,Meng W,Liu B,Luo Y,Liu M,Bai Y,Abdul-Ghani MA,Li R,Wu J,Zeng R,Zhou Z,Dong LQ,Liu F

Endoplasmic reticulum (ER) localization is critical for DsbA-L protein to suppress ER stress and adiponectin down-regulation in adipocytes.
The Journal of biological chemistry290(16)10143-8(2015 Apr)
Liu M,Chen H,Wei L,Hu D,Dong K,Jia W,Dong LQ,Liu F

Negative regulation of DsbA-L gene expression by the transcription factor Sp1.
Diabetes63(12)4165-71(2014 Dec)
Fang Q,Yang W,Li H,Hu W,Chen L,Jiang S,Dong K,Song Q,Wang C,Chen S,Liu F,Jia W

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Gstk1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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