Ppp1r14c cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp1r14c
Entrez Gene ID	76142
Full Name	protein phosphatase 1, regulatory (inhibitor) subunit 14c
Synonyms	6330514J04Rik,KEPI
General protein information	Preferred Names protein phosphatase 1, regulatory (inhibitor) subunit 14c Names protein phosphatase 1 regulatory subunit 14C PKC-potentiated PP1 inhibitory protein kinase-enhanced PP1 inhibitor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512490.4	XP_006512553.1	protein phosphatase 1 regulatory subunit 14C isoform X2
XM_030245354.1	XP_030101214.1	protein phosphatase 1 regulatory subunit 14C isoform X3
XM_006512489.2	XP_006512552.1	protein phosphatase 1 regulatory subunit 14C isoform X1
XM_006512490.3	XP_006512553.1	protein phosphatase 1 regulatory subunit 14C isoform X2
NM_133485.2	NP_597844.1	protein phosphatase 1 regulatory subunit 14C
XM_006512489.3	XP_006512552.1	protein phosphatase 1 regulatory subunit 14C isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP1R14C	XP_005615562.1
Bos taurus (cattle)	LOC783497	XP_002692993.1
Mus musculus (house mouse)	Ppp1r14c	NP_597844.1
Rattus norvegicus (Norway rat)	Ppp1r14c	NP_596916.1
Homo sapiens (human)	PPP1R14C	NP_112211.1
Bos taurus (cattle)	PPP1R14C	NP_001071536.1
Pan troglodytes (chimpanzee)	PPP1R14C	XP_001135266.2
Danio rerio (zebrafish)	ppp1r14c	NP_001025134.1
Danio rerio (zebrafish)	LOC100330870	XP_002666467.1
Xenopus tropicalis (tropical clawed frog)	ppp1r14c	XP_002931538.1

Gene Ontology
Bibliography

Related articles in PubMed

Retromer in Osteoblasts Interacts With Protein Phosphatase 1 Regulator Subunit 14C, Terminates Parathyroid Hormone's Signaling, and Promotes Its Catabolic Response.
Xiong L, Xia WF, Tang FL, Pan JX, Mei L, Xiong WC
EBioMedicine945-60(2016 Jul)

Effect of KEPI (Ppp1r14c) deletion on morphine analgesia and tolerance in mice of different genetic backgrounds: when a knockout is near a relevant quantitative trait locus.
Drgonova J, Zimonjic DB, Hall FS, Uhl GR
Neuroscience165(3)882-95(2010 Feb)

Efficient capture of cardiogenesis-associated genes expressed in ES cells.
Terami H, Hidaka K, Shirai M, Narumiya H, Kuroyanagi T, Arai Y, Aburatani H, Morisaki T
Biochemical and biophysical research communications355(1)47-53(2007 Mar)

Mouse brain localization of the protein kinase C-enhanced phosphatase 1 inhibitor KEPI (kinase C-enhanced PP1 inhibitor).
Gong JP, Liu QR, Zhang PW, Wang Y, Uhl GR
Neuroscience132(3)713-27(2005)

KEPI, a PKC-dependent protein phosphatase 1 inhibitor regulated by morphine.
Liu QR, Zhang PW, Zhen Q, Walther D, Wang XB, Uhl GR
The Journal of biological chemistry277(15)13312-20(2002 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the data presented in this manuscript demonstrate a critical function of VPS35 in regulating PTH1R trafficking. This event and VPS35-interaction with PPP1R14C appear to be essential for turning off PTH1R's endosomal signaling, and promoting PTH1R-mediated catabolic response and bone remodeling.
Title: Retromer in Osteoblasts Interacts With Protein Phosphatase 1 Regulator Subunit 14C, Terminates Parathyroid Hormone's Signaling, and Promotes Its Catabolic Response.

The expression patterns point to possible roles for KEPI in regulating protein dephosphorylation by inhibiting PP1 activities in a number of brain pathways.
Title: Mouse brain localization of the protein kinase C-enhanced phosphatase 1 inhibitor KEPI (kinase C-enhanced PP1 inhibitor).

PPP1R14C gene encodes a PKC-potentiated inhibitory protein, KEPI, for type-1 Ser/Thr protein phosphatase (PP1). KEPI mRNA and protein are enriched in brain, heart and skeletal muscle.
Title: KEPI, a PKC-dependent protein phosphatase 1 inhibitor regulated by morphine.

The following Ppp1r14c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp1r14c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10027	NM_133485.2 Latest version!	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu43686	XM_006512489.3 Latest version!	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu43687	XM_006512490.4 Latest version!	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu85547	XM_030245354.1 Latest version!	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu43687	XM_006512490.3	Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 14c (Ppp1r14c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu43686	XM_006512489.2	Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 14c (Ppp1r14c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp1r14c ( XM_006512489.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512552.1 Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 14c (Ppp1r14c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43686
Clone ID Related Accession (Same CDS sequence)	XM_006512489.2 , XM_006512489.3
Accession Version	XM_006512489.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1 regulatory subunit 14C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512489.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGCTTGGAT ATTGCTCTAT CAAACATCTC AATGAAGAGG GAAGGCTACA TGTTGGAGAA 
GAAGAAATGC CAGATGTAGA AATTGACATT GATGATCTTC TTGATGCCAA CAGCGAGGAG 
GAAAGAGCTT CGAAATTACA GGAAGCTCTC GTAGACTGCT ATAAACCAAC AGAGGAATTC 
ATCAGAGAGC TGCTCTCCCG GATAAGAGGC ATGAGGAAGC TGAGCCCTCC GCAGAAGAAG 
AGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512552.1
CDS	597..1145
Translation	MSVVTGGGEAAGGGGGGGARVFFQSPRGGTGGSPGSSSSSGSSREDSAPVTTVAAAGQVQQQQRRHQQGKVTVKYDRKELRKRLVLEEWIVEQLGQLYGCELGYCSIKHLNEEGRLHVGEEEMPDVEIDIDDLLDANSEEERASKLQEALVDCYKPTEEFIRELLSRIRGMRKLSPPQKKSV

Target ORF information:

RefSeq Version	XM_006512489.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 14c (Ppp1r14c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512489.2

ORF Insert Sequence:

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGCTTGGAT ATTGCTCTAT CAAACATCTC AATGAAGAGG GAAGGCTACA TGTTGGAGAA 
GAAGAAATGC CAGATGTAGA AATTGACATT GATGATCTTC TTGATGCCAA CAGCGAGGAG 
GAAAGAGCTT CGAAATTACA GGAAGCTCTC GTAGACTGCT ATAAACCAAC AGAGGAATTC 
ATCAGAGAGC TGCTCTCCCG GATAAGAGGC ATGAGGAAGC TGAGCCCTCC GCAGAAGAAG 
AGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r14c ( XM_006512490.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512553.1 Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 14c (Ppp1r14c), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43687
Clone ID Related Accession (Same CDS sequence)	XM_006512490.4 , XM_006512490.3
Accession Version	XM_006512490.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1 regulatory subunit 14C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGCTTGGAT ATTGCTCTAT CAAACATCTC AATGAAGAGG GAAGGCTACA TGTTGGAGAA 
GAAGAAATGC CAGATGTAGA AATTGACATT GATGATCTTC TTGATGCCAA CAGCGAGGAG 
GAAAGAGCTT CGAAATTACA GGAAGCTCTC GTAGACTGCT ATAAACCAAC AGAGGGTGAA 
GAGATCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512553.1
CDS	597..1088
Translation	MSVVTGGGEAAGGGGGGGARVFFQSPRGGTGGSPGSSSSSGSSREDSAPVTTVAAAGQVQQQQRRHQQGKVTVKYDRKELRKRLVLEEWIVEQLGQLYGCELGYCSIKHLNEEGRLHVGEEEMPDVEIDIDDLLDANSEEERASKLQEALVDCYKPTEGEEIT

Target ORF information:

RefSeq Version	XM_006512490.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 14c (Ppp1r14c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512490.3

ORF Insert Sequence:

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGCTTGGAT ATTGCTCTAT CAAACATCTC AATGAAGAGG GAAGGCTACA TGTTGGAGAA 
GAAGAAATGC CAGATGTAGA AATTGACATT GATGATCTTC TTGATGCCAA CAGCGAGGAG 
GAAAGAGCTT CGAAATTACA GGAAGCTCTC GTAGACTGCT ATAAACCAAC AGAGGGTGAA 
GAGATCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r14c ( NM_133485.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_597844.1 Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), mRNA.

Price & Availability↑

CloneID	OMu10027
Clone ID Related Accession (Same CDS sequence)	NM_133485.2
Accession Version	NM_133485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1 regulatory subunit 14C
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC162384.6 and AC155833.10. On or before Aug 8, 2008 this sequence version replaced XM_001471789.1, NM_133485.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF407167.2, AK085997.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGGAAGAAG AAATGCCAGA TGTAGAAATT GACATTGATG ATCTTCTTGA TGCCAACAGC 
GAGGAGGAAA GAGCTTCGAA ATTACAGGAA GCTCTCGTAG ACTGCTATAA ACCAACAGAG 
GAATTCATCA GAGAGCTGCT CTCCCGGATA AGAGGCATGA GGAAGCTGAG CCCTCCGCAG 
AAGAAGAGCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_597844.1
CDS	518..1012
Translation	MSVVTGGGEAAGGGGGGGARVFFQSPRGGTGGSPGSSSSSGSSREDSAPVTTVAAAGQVQQQQRRHQQGKVTVKYDRKELRKRLVLEEWIVEQLGQLYGCEEEEMPDVEIDIDDLLDANSEEERASKLQEALVDCYKPTEEFIRELLSRIRGMRKLSPPQKKSV

Target ORF information:

RefSeq Version	NM_133485.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133485.2

ORF Insert Sequence:

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGGAAGAAG AAATGCCAGA TGTAGAAATT GACATTGATG ATCTTCTTGA TGCCAACAGC 
GAGGAGGAAA GAGCTTCGAA ATTACAGGAA GCTCTCGTAG ACTGCTATAA ACCAACAGAG 
GAATTCATCA GAGAGCTGCT CTCCCGGATA AGAGGCATGA GGAAGCTGAG CCCTCCGCAG 
AAGAAGAGCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r14c ( XM_006512489.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512552.1 Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43686
Clone ID Related Accession (Same CDS sequence)	XM_006512489.2 , XM_006512489.3
Accession Version	XM_006512489.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1 regulatory subunit 14C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512489.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGCTTGGAT ATTGCTCTAT CAAACATCTC AATGAAGAGG GAAGGCTACA TGTTGGAGAA 
GAAGAAATGC CAGATGTAGA AATTGACATT GATGATCTTC TTGATGCCAA CAGCGAGGAG 
GAAAGAGCTT CGAAATTACA GGAAGCTCTC GTAGACTGCT ATAAACCAAC AGAGGAATTC 
ATCAGAGAGC TGCTCTCCCG GATAAGAGGC ATGAGGAAGC TGAGCCCTCC GCAGAAGAAG 
AGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512552.1
CDS	639..1187
Translation	MSVVTGGGEAAGGGGGGGARVFFQSPRGGTGGSPGSSSSSGSSREDSAPVTTVAAAGQVQQQQRRHQQGKVTVKYDRKELRKRLVLEEWIVEQLGQLYGCELGYCSIKHLNEEGRLHVGEEEMPDVEIDIDDLLDANSEEERASKLQEALVDCYKPTEEFIRELLSRIRGMRKLSPPQKKSV

Target ORF information:

RefSeq Version	XM_006512489.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512489.3

ORF Insert Sequence:

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGCTTGGAT ATTGCTCTAT CAAACATCTC AATGAAGAGG GAAGGCTACA TGTTGGAGAA 
GAAGAAATGC CAGATGTAGA AATTGACATT GATGATCTTC TTGATGCCAA CAGCGAGGAG 
GAAAGAGCTT CGAAATTACA GGAAGCTCTC GTAGACTGCT ATAAACCAAC AGAGGAATTC 
ATCAGAGAGC TGCTCTCCCG GATAAGAGGC ATGAGGAAGC TGAGCCCTCC GCAGAAGAAG 
AGCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r14c ( XM_006512490.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512553.1 Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43687
Clone ID Related Accession (Same CDS sequence)	XM_006512490.4 , XM_006512490.3
Accession Version	XM_006512490.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1 regulatory subunit 14C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512490.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGCTTGGAT ATTGCTCTAT CAAACATCTC AATGAAGAGG GAAGGCTACA TGTTGGAGAA 
GAAGAAATGC CAGATGTAGA AATTGACATT GATGATCTTC TTGATGCCAA CAGCGAGGAG 
GAAAGAGCTT CGAAATTACA GGAAGCTCTC GTAGACTGCT ATAAACCAAC AGAGGGTGAA 
GAGATCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512553.1
CDS	649..1140
Translation	MSVVTGGGEAAGGGGGGGARVFFQSPRGGTGGSPGSSSSSGSSREDSAPVTTVAAAGQVQQQQRRHQQGKVTVKYDRKELRKRLVLEEWIVEQLGQLYGCELGYCSIKHLNEEGRLHVGEEEMPDVEIDIDDLLDANSEEERASKLQEALVDCYKPTEGEEIT

Target ORF information:

RefSeq Version	XM_006512490.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512490.4

ORF Insert Sequence:

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGCTTGGAT ATTGCTCTAT CAAACATCTC AATGAAGAGG GAAGGCTACA TGTTGGAGAA 
GAAGAAATGC CAGATGTAGA AATTGACATT GATGATCTTC TTGATGCCAA CAGCGAGGAG 
GAAAGAGCTT CGAAATTACA GGAAGCTCTC GTAGACTGCT ATAAACCAAC AGAGGGTGAA 
GAGATCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r14c ( XM_030245354.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101214.1 Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), transcript variant X3, mRNA.

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CloneID	OMu85547
Clone ID Related Accession (Same CDS sequence)	XM_030245354.1
Accession Version	XM_030245354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1 regulatory subunit 14C isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGGAAGAAG AAATGCCAGA TGTAGAAATT GACATTGATG ATCTTCTTGA TGCCAACAGC 
GAGGAGGAAA GAGCTTCGAA ATTACAGGAA GCTCTCGTAG ACTGCTATAA ACCAACAGAG 
GGTGAAGAGA TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101214.1
CDS	694..1131
Translation	MSVVTGGGEAAGGGGGGGARVFFQSPRGGTGGSPGSSSSSGSSREDSAPVTTVAAAGQVQQQQRRHQQGKVTVKYDRKELRKRLVLEEWIVEQLGQLYGCEEEEMPDVEIDIDDLLDANSEEERASKLQEALVDCYKPTEGEEIT

Target ORF information:

RefSeq Version	XM_030245354.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14C (Ppp1r14c), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245354.1

ORF Insert Sequence:

ATGTCGGTGG TGACTGGCGG CGGTGAGGCG GCCGGTGGCG GCGGCGGCGG CGGTGCGCGT 
GTCTTCTTCC AGAGTCCCCG CGGTGGAACC GGCGGCAGCC CAGGATCCAG CAGCAGCTCC 
GGCTCCTCAA GGGAGGACTC GGCGCCGGTG ACTACGGTGG CCGCCGCCGG GCAAGTGCAA 
CAGCAACAAC GACGCCACCA GCAGGGAAAA GTGACAGTGA AATACGACCG TAAGGAGCTT 
CGTAAGCGGT TGGTGCTGGA AGAATGGATC GTGGAGCAGC TGGGTCAGCT GTACGGCTGC 
GAGGAAGAAG AAATGCCAGA TGTAGAAATT GACATTGATG ATCTTCTTGA TGCCAACAGC 
GAGGAGGAAA GAGCTTCGAA ATTACAGGAA GCTCTCGTAG ACTGCTATAA ACCAACAGAG 
GGTGAAGAGA TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retromer in Osteoblasts Interacts With Protein Phosphatase 1 Regulator Subunit 14C, Terminates Parathyroid Hormone's Signaling, and Promotes Its Catabolic Response.
EBioMedicine945-60(2016 Jul)
Xiong L,Xia WF,Tang FL,Pan JX,Mei L,Xiong WC

Effect of KEPI (Ppp1r14c) deletion on morphine analgesia and tolerance in mice of different genetic backgrounds: when a knockout is near a relevant quantitative trait locus.
Neuroscience165(3)882-95(2010 Feb)
Drgonova J,Zimonjic DB,Hall FS,Uhl GR

Efficient capture of cardiogenesis-associated genes expressed in ES cells.
Biochemical and biophysical research communications355(1)47-53(2007 Mar)
Terami H,Hidaka K,Shirai M,Narumiya H,Kuroyanagi T,Arai Y,Aburatani H,Morisaki T

Mouse brain localization of the protein kinase C-enhanced phosphatase 1 inhibitor KEPI (kinase C-enhanced PP1 inhibitor).
Neuroscience132(3)713-27(2005)
Gong JP,Liu QR,Zhang PW,Wang Y,Uhl GR

KEPI, a PKC-dependent protein phosphatase 1 inhibitor regulated by morphine.
The Journal of biological chemistry277(15)13312-20(2002 Apr)
Liu QR,Zhang PW,Zhen Q,Walther D,Wang XB,Uhl GR

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Ppp1r14c cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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