gRNAs
The following Depdc1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Depdc1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu11885 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001172093.1 | |
| Accession Version | NM_001172093.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | DEP domain-containing protein 1A isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB857645.1, AK050456.1, AK146458.1 and CK340717.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK050456.1, AK088719.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGCGTC AGGGTGCTCC TCTTGGACCT TACCGGGCCA CCAAGCTGTG GAATGAAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001165564.1 |
| CDS | 87..1676 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001172093.1 |
1 | ATGGAGCGTC AGGGTGCTCC TCTTGGACCT TACCGGGCCA CCAAGCTGTG GAATGAAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu11883 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001172092.1 | |
| Accession Version | NM_001172092.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | DEP domain-containing protein 1A isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB857645.1, AK146458.1, AK050456.1 and CK340717.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK146458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGCGTC AGGGTGCTCC TCTTGGACCT TACCGGGCCA CCAAGCTGTG GAATGAAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001165563.1 |
| CDS | 87..2507 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001172092.1 |
1 | ATGGAGCGTC AGGGTGCTCC TCTTGGACCT TACCGGGCCA CCAAGCTGTG GAATGAAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu07090 | |
| Clone ID Related Accession (Same CDS sequence) | NM_029523.3 | |
| Accession Version | NM_029523.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | DEP domain-containing protein 1A isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB857645.1, AK146458.1, AK050456.1 and CK340717.1. On Feb 3, 2010 this sequence version replaced NM_029523.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks a two amino acid segment near the C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC023934.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGCGTC AGGGTGCTCC TCTTGGACCT TACCGGGCCA CCAAGCTGTG GAATGAAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_083799.2 |
| CDS | 87..2501 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_029523.3 |
1 | ATGGAGCGTC AGGGTGCTCC TCTTGGACCT TACCGGGCCA CCAAGCTGTG GAATGAAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells. |
Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method. |
