gRNAs
The following Brsk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brsk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu15777 | |
| Clone ID Related Accession (Same CDS sequence) | NM_029426.2 | |
| Accession Version | NM_029426.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-25 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase BRSK2 isoform alpha | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772165.15 and AL603836.13. On Nov 17, 2006 this sequence version replaced NM_029426.1. Transcript Variant: This variant (1) lacks an alternate in-frame 3' exon and uses an alternate splice junction at the 5' end of the last exon compared to variant 3. The resulting isoform (alpha) has a shorter and distinct C-terminus compared to isoform gamma. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY660739.1, AY533672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_083702.1 |
| CDS | 336..2297 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_029426.2 |
1 | ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu15811 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001009930.3 | |
| Accession Version | NM_001009930.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-13 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase BRSK2 isoform gamma | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772165.15, AK158574.1, AL603836.13 and BE986320.1. On Oct 13, 2012 this sequence version replaced NM_001009930.2. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (gamma). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY533674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001009930.1 |
| CDS | 336..2495 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001009930.3 |
1 | ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu15973 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001009929.3 | |
| Accession Version | NM_001009929.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-13 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase BRSK2 isoform beta | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772165.15, AK158574.1 and AL603836.13. On Oct 13, 2012 this sequence version replaced NM_001009929.2. Transcript Variant: This variant (2) lacks a 3' coding exon and contains an additional 3' UTR exon and uses an alternate splice junction at the 5' end of the last exon compared to isoform 3. The resulting isoform (beta) has a shorter and distinct C-terminus compared to isoform gamma. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY533673.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001009929.1 |
| CDS | 336..2363 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001009929.3 |
1 | ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu16581 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001276763.1 | |
| Accession Version | NM_001276763.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-13 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase BRSK2 isoform delta | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772165.15 and AL603836.13. Transcript Variant: This variant (4) has multiple differences in the coding region and 3' UTR, compared to variant 3. The resulting protein (isoform delta) is shorter than isoform gamma. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JF707772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001263692.1 |
| CDS | 336..2363 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001276763.1 |
1 | ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Structural insight into the mechanism of synergistic autoinhibition of SAD kinases. |
LKB1 and AMPK regulate synaptic remodeling in old age. |
