Eif4b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif4b
Entrez Gene ID	75705
Full Name	eukaryotic translation initiation factor 4B
Synonyms	2310046H11Rik,AL024095,C85189,Eif4a2
General protein information	Preferred Names eukaryotic translation initiation factor 4B Names eukaryotic translation initiation factor 4B eIF-4B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_145625.3	NP_663600.2	eukaryotic translation initiation factor 4B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF4B	XP_001142097.3
Canis lupus familiaris (dog)	EIF4B	XP_005636844.1
Danio rerio (zebrafish)	eif4bb	NP_999883.1
Homo sapiens (human)	EIF4B	NP_001408.2
Rattus norvegicus (Norway rat)	Eif4b	NP_001008325.1
Xenopus tropicalis (tropical clawed frog)	eif4b	NP_001005064.1
Danio rerio (zebrafish)	eif4ba	NP_001092707.2
Bos taurus (cattle)	EIF4B	NP_001030200.1
Mus musculus (house mouse)	Eif4b	NP_663600.2
	EIF4B	XP_001101380.2
Gallus gallus (chicken)	EIF4B	XP_003643456.2

Gene Ontology
Bibliography

Related articles in PubMed

C/EBP?-LAP*/LAP Expression Is Mediated by RSK/eIF4B-Dependent Signalling and Boosted by Increased Protein Stability in Models of Monocytic Differentiation.
Huber R, Panterodt T, Welz B, Christmann M, Friesenhagen J, Westphal A, Pietsch D, Brand K
PloS one10(12)e0144338(2015)

Role of p70S6K1-mediated phosphorylation of eIF4B and PDCD4 proteins in the regulation of protein synthesis.
Dennis MD, Jefferson LS, Kimball SR
The Journal of biological chemistry287(51)42890-9(2012 Dec)

Interferon-dependent engagement of eukaryotic initiation factor 4B via S6 kinase (S6K)- and ribosomal protein S6K-mediated signals.
Kroczynska B, Kaur S, Katsoulidis E, Majchrzak-Kita B, Sassano A, Kozma SC, Fish EN, Platanias LC
Molecular and cellular biology29(10)2865-75(2009 May)

AGC kinases regulate phosphorylation and activation of eukaryotic translation initiation factor 4B.
van Gorp AG, van der Vos KE, Brenkman AB, Bremer A, van den Broek N, Zwartkruis F, Hershey JW, Burgering BM, Calkhoven CF, Coffer PJ
Oncogene28(1)95-106(2009 Jan)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PKR inhibition reduced the expression of eIF4B and C/EBPbeta in an eIF2alpha-independent manner
Title: C/EBP?-LAP*/LAP Expression Is Mediated by RSK/eIF4B-Dependent Signalling and Boosted by Increased Protein Stability in Models of Monocytic Differentiation.

Maintenance of eIF4E interaction with eIF4G was not by itself sufficient to sustain global rates of protein synthesis in the absence of mTORC1 signaling to p70S6K1; phosphorylation of both eIF4B and PDCD4 was additionally required.
Title: Role of p70S6K1-mediated phosphorylation of eIF4B and PDCD4 proteins in the regulation of protein synthesis.

C-loops bind to eIF4B and prevent the factor's interaction with 18S rRNA of the small ribosomal subunit.
Title: Dual nature of translational control by regulatory BC RNAs.

Results establish critical roles for S6K and RSK in the induction of IFN-dependent biological effects and define a key regulatory role for eIF4B as a common mediator and integrator of IFN-generated signals from these kinases.
Title: Interferon-dependent engagement of eukaryotic initiation factor 4B via S6 kinase (S6K)- and ribosomal protein S6K-mediated signals.

These data provide novel insights into complex multikinase regulation of eIF4B phosphorylation and reveal an important mechanism by which PKB can regulate translation, potentially critical for the transforming capacity of this AGC kinase family member.
Title: AGC kinases regulate phosphorylation and activation of eukaryotic translation initiation factor 4B.

The following Eif4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15433	NM_145625.3 Latest version!	Mus musculus eukaryotic translation initiation factor 4B (Eif4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$356.30 $509.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif4b ( NM_145625.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663600.2 Mus musculus eukaryotic translation initiation factor 4B (Eif4b), mRNA.

Price & Availability↑

CloneID	OMu15433
Clone ID Related Accession (Same CDS sequence)	NM_145625.3
Accession Version	NM_145625.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CD563487.1, AK045250.1 and CO043713.1. On Feb 7, 2008 this sequence version replaced NM_145625.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036293.1, AK148012.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCT CAGCAAAAAA GAAGAATAAG AAGGGGAAGA CCATCTCCCT AACGGACTTT 
CTAGCTGAGG ATGGAGGAAC TGGTGGAGGA AGCACCTATG TCCCCAAACC AGTCAGCTGG 
GCTGATGAAA CAGACGATCT GGAAGGAGAT GTGTCAACAA CTTGGCATAG TAACGATGAT 
GACGTGTACA GGGCGCCTCC AATTGACCGT TCCATCCTTC CCACTGCTCC ACGGGCTGCT 
CGGGAACCCA ATATTGACCG GAGCCGTCTT CCCAAGTCGC CACCCTACAC TGCTTTCCTA 
GGGAATCTGC CCTATGATGT GACAGAAGAC TCCATTAAGG ATTTCTTTAG AGGATTAAAT 
ATCAGCGCTG TACGCTTACC ACGGGAACCC AGCAATCCAG ACAGGTTGAA AGGTTTCGGC 
TACGCAGAAT TTGAGGACCT GGATTCTCTG CTCAGTGCTC TGAGTCTCAA TGAAGAGTCT 
CTAGGTAACA GGAGAATTCG TGTGGATGTT GCTGATCAAG CACAGGATAA AGACAGGGAT 
GACCGTTCTT TTGGTCGAGA TAGAAATCGG GATTCTGACA AAACAGACAC AGACTGGAGG 
GCCCGTCCCA CCACAGACAG TTTTGATGAC TACCCACCTA GAAGAGGCGA TGATAGCTTT 
GGAGACAAGT ATCGAGATCG TTACGATTCA GACCGGTATC GGGATGGGTA TAGGGACGGA 
TATCGGGACG GCCCACGCAG AGACATGGAC CGCTATGGGG GCCGGGATCG CTATGATGAC 
CGAGGCAGCA GAGACTATGA CCGAGGCTAT GACTCCAGGA TAGGCAGTGG CAGAAGGGCA 
TTTGGAAGTG GGTACCGGAG AGATGATGAC TACAGAGGAG GTGGGGACCG CTATGAAGAC 
CGCTATGACA GACGGGATGA TCGGTCGTGG AGCTCCAGGG ATGACTACTC TCGGGATGAT 
TATAGGCGTG ATGACAGAGG TCCCCCCCAG AGACCCAGAC TGAACCTCAA GCCTCGAAGC 
GCTCCTAAGG AGGATGACGC CTCCGCCAGC ACCTCCCAGT CCAGCCGGGC AGCCTCCATC 
TTTGGAGGGG CGAAGCCTGT TGACACAGCT GCTAGGGAAA GAGAAGTAGA GGAGCGGCTA 
CAGAAGGAGC AGGAGAAGCT GCAGCGTCAG CTGGATGAGC CAAAACTAGA CCGCCGGCCC 
CGGGAGAGAC ACCCAAGCTG GCGAAGTGAA GAAACTCAGG AAAGAGAACG GTCAAGGACA 
GGAAGTGAGT CATCGCAGAC TGGGGCCTCA GCCACATCTG GCAGAAATAC ACGAAGGAGA 
GAGAGTGAGA AGTCTCTAGA AAATGAAACC CTCAATAAAG AAGAAGACTG TCACTCTCCA 
ACCTCTAAGC CTCCTAAACC TGACCAGCCT CTAAAGGTAA TGCCAGCCCC TCCACCAAAG 
GAGAATGCGT GGGTGAAGCG AAGCTCTAAC CCTCCTGCCC GATCTCAGAG CTCAGACACA 
GAGCAGCCGT CCCCTACAAG TGGTGGAGGG AAAGTAGCTG CAGTCCAGCC CCCTGAGGAA 
GGACCATCAA GAAAAGATGG AAATAAAGTG GATGTGGTGG GTGCCACACA AGGCCAAGCT 
GGAAGCTGCA GCCGTGGTCC CGGGGATGGA GGGAGCAGAG ACCACTGGAA GGACTTGGAT 
AGGAAGGATG GCAAAAAAGA TCAAGACTCC AGATCTGCGC CTGAGCCAAA GAAACCTGAG 
GAGAACCCAG CCTCTAAGTT CAGCTCTGCA AGCAAGTACG CTGCTCTGTC TGTGGATGGC 
GAGGATGAGG ATGAGGGCGA CGACTGCACT GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663600.2
CDS	179..2014
Translation	MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVPKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEDSIKDFFRGLNISAVRLPREPSNPDRLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPTTDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPRRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPRLNLKPRSAPKEDDASASTSQSSRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLDRRPRERHPSWRSEETQERERSRTGSESSQTGASATSGRNTRRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQPSPTSGGGKVAAVQPPEEGPSRKDGNKVDVVGATQGQAGSCSRGPGDGGSRDHWKDLDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDEDEGDDCTE

Target ORF information:

RefSeq Version	NM_145625.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4B (Eif4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145625.3

ORF Insert Sequence:

ATGGCGGCCT CAGCAAAAAA GAAGAATAAG AAGGGGAAGA CCATCTCCCT AACGGACTTT 
CTAGCTGAGG ATGGAGGAAC TGGTGGAGGA AGCACCTATG TCCCCAAACC AGTCAGCTGG 
GCTGATGAAA CAGACGATCT GGAAGGAGAT GTGTCAACAA CTTGGCATAG TAACGATGAT 
GACGTGTACA GGGCGCCTCC AATTGACCGT TCCATCCTTC CCACTGCTCC ACGGGCTGCT 
CGGGAACCCA ATATTGACCG GAGCCGTCTT CCCAAGTCGC CACCCTACAC TGCTTTCCTA 
GGGAATCTGC CCTATGATGT GACAGAAGAC TCCATTAAGG ATTTCTTTAG AGGATTAAAT 
ATCAGCGCTG TACGCTTACC ACGGGAACCC AGCAATCCAG ACAGGTTGAA AGGTTTCGGC 
TACGCAGAAT TTGAGGACCT GGATTCTCTG CTCAGTGCTC TGAGTCTCAA TGAAGAGTCT 
CTAGGTAACA GGAGAATTCG TGTGGATGTT GCTGATCAAG CACAGGATAA AGACAGGGAT 
GACCGTTCTT TTGGTCGAGA TAGAAATCGG GATTCTGACA AAACAGACAC AGACTGGAGG 
GCCCGTCCCA CCACAGACAG TTTTGATGAC TACCCACCTA GAAGAGGCGA TGATAGCTTT 
GGAGACAAGT ATCGAGATCG TTACGATTCA GACCGGTATC GGGATGGGTA TAGGGACGGA 
TATCGGGACG GCCCACGCAG AGACATGGAC CGCTATGGGG GCCGGGATCG CTATGATGAC 
CGAGGCAGCA GAGACTATGA CCGAGGCTAT GACTCCAGGA TAGGCAGTGG CAGAAGGGCA 
TTTGGAAGTG GGTACCGGAG AGATGATGAC TACAGAGGAG GTGGGGACCG CTATGAAGAC 
CGCTATGACA GACGGGATGA TCGGTCGTGG AGCTCCAGGG ATGACTACTC TCGGGATGAT 
TATAGGCGTG ATGACAGAGG TCCCCCCCAG AGACCCAGAC TGAACCTCAA GCCTCGAAGC 
GCTCCTAAGG AGGATGACGC CTCCGCCAGC ACCTCCCAGT CCAGCCGGGC AGCCTCCATC 
TTTGGAGGGG CGAAGCCTGT TGACACAGCT GCTAGGGAAA GAGAAGTAGA GGAGCGGCTA 
CAGAAGGAGC AGGAGAAGCT GCAGCGTCAG CTGGATGAGC CAAAACTAGA CCGCCGGCCC 
CGGGAGAGAC ACCCAAGCTG GCGAAGTGAA GAAACTCAGG AAAGAGAACG GTCAAGGACA 
GGAAGTGAGT CATCGCAGAC TGGGGCCTCA GCCACATCTG GCAGAAATAC ACGAAGGAGA 
GAGAGTGAGA AGTCTCTAGA AAATGAAACC CTCAATAAAG AAGAAGACTG TCACTCTCCA 
ACCTCTAAGC CTCCTAAACC TGACCAGCCT CTAAAGGTAA TGCCAGCCCC TCCACCAAAG 
GAGAATGCGT GGGTGAAGCG AAGCTCTAAC CCTCCTGCCC GATCTCAGAG CTCAGACACA 
GAGCAGCCGT CCCCTACAAG TGGTGGAGGG AAAGTAGCTG CAGTCCAGCC CCCTGAGGAA 
GGACCATCAA GAAAAGATGG AAATAAAGTG GATGTGGTGG GTGCCACACA AGGCCAAGCT 
GGAAGCTGCA GCCGTGGTCC CGGGGATGGA GGGAGCAGAG ACCACTGGAA GGACTTGGAT 
AGGAAGGATG GCAAAAAAGA TCAAGACTCC AGATCTGCGC CTGAGCCAAA GAAACCTGAG 
GAGAACCCAG CCTCTAAGTT CAGCTCTGCA AGCAAGTACG CTGCTCTGTC TGTGGATGGC 
GAGGATGAGG ATGAGGGCGA CGACTGCACT GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

C/EBP?-LAP*/LAP Expression Is Mediated by RSK/eIF4B-Dependent Signalling and Boosted by Increased Protein Stability in Models of Monocytic Differentiation.
PloS one10(12)e0144338(2015)
Huber R,Panterodt T,Welz B,Christmann M,Friesenhagen J,Westphal A,Pietsch D,Brand K

Role of p70S6K1-mediated phosphorylation of eIF4B and PDCD4 proteins in the regulation of protein synthesis.
The Journal of biological chemistry287(51)42890-9(2012 Dec)
Dennis MD,Jefferson LS,Kimball SR

Interferon-dependent engagement of eukaryotic initiation factor 4B via S6 kinase (S6K)- and ribosomal protein S6K-mediated signals.
Molecular and cellular biology29(10)2865-75(2009 May)
Kroczynska B,Kaur S,Katsoulidis E,Majchrzak-Kita B,Sassano A,Kozma SC,Fish EN,Platanias LC

AGC kinases regulate phosphorylation and activation of eukaryotic translation initiation factor 4B.
Oncogene28(1)95-106(2009 Jan)
van Gorp AG,van der Vos KE,Brenkman AB,Bremer A,van den Broek N,Zwartkruis F,Hershey JW,Burgering BM,Calkhoven CF,Coffer PJ

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

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Eif4b cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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