Pik3r4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pik3r4
Entrez Gene ID	75669
Full Name	phosphoinositide-3-kinase regulatory subunit 4
Synonyms	2210010O15Rik,C730038E05Rik,C85833,D9Ertd418e,Vps15,p150
General protein information	Preferred Names phosphoinositide-3-kinase regulatory subunit 4 Names phosphoinositide 3-kinase regulatory subunit 4 PI3-kinase regulatory subunit 4 phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 4, p150
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F1\|9 56.79 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081309.1	NP_001074778.1	phosphoinositide 3-kinase regulatory subunit 4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PIK3R4	XP_004939498.1
Danio rerio (zebrafish)	pik3r4	XP_001922676.1
Oryza sativa (rice)	Os02g0796700	NP_001048391.1
Canis lupus familiaris (dog)	PIK3R4	XP_534260.1
Bos taurus (cattle)	PIK3R4	NP_001093784.1
Eremothecium gossypii	AGOS_ADL316C	NP_983780.2
Magnaporthe oryzae (rice blast fungus)	MGG_06100	XP_003711966.1
Neurospora crassa	NCU06626	XP_962368.2
	PIK3R4	XP_002802990.1
Mus musculus (house mouse)	Pik3r4	NP_001074778.1
Rattus norvegicus (Norway rat)	Pik3r4	NP_001102247.1
Saccharomyces cerevisiae (baker's yeast)	VPS15	NP_009655.2
Schizosaccharomyces pombe (fission yeast)	ppk19	NP_595288.1
Xenopus tropicalis (tropical clawed frog)	pik3r4	NP_001107701.1
Pan troglodytes (chimpanzee)	PIK3R4	XP_516746.2
Drosophila melanogaster (fruit fly)	ird1	NP_649868.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000602	XP_310476.5
Arabidopsis thaliana (thale cress)	AT4G29380	NP_194667.1
Homo sapiens (human)	PIK3R4	NP_055417.1
Kluyveromyces lactis	KLLA0B11924g	XP_452060.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.
Gstrein T, Edwards A, P?istoupilov? A, Leca I, Breuss M, Pilat-Carotta S, Hansen AH, Tripathy R, Traunbauer AK, Hochstoeger T, Rosoklija G, Repic M, Landler L, Str?neck? V, D?rnberger G, Keane TM, Zuber J, Adams DJ, Flint J, Honzik T, Gut M, Beltran S, Mechtler K, Sherr E, Kmoch S, Gut I, Keays DA
Nature neuroscience(2018 Jan)

Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling.
Nemazanyy I, Montagnac G, Russell RC, Morzyglod L, Burnol AF, Guan KL, Pende M, Panasyuk G
Nature communications68283(2015 Sep)

Connexins modulate autophagosome biogenesis.
Bejarano E, Yuste A, Patel B, Stout RF Jr, Spray DC, Cuervo AM
Nature cell biology16(5)401-14(2014 May)

Functional interaction between autophagy and ciliogenesis.
Pampliega O, Orhon I, Patel B, Sridhar S, D?az-Carretero A, Beau I, Codogno P, Satir BH, Satir P, Cuervo AM
Nature502(7470)194-200(2013 Oct)

Defects of Vps15 in skeletal muscles lead to autophagic vacuolar myopathy and lysosomal disease.
Nemazanyy I, Blaauw B, Paolini C, Caillaud C, Protasi F, Mueller A, Proikas-Cezanne T, Russell RC, Guan KL, Nishino I, Sandri M, Pende M, Panasyuk G
EMBO molecular medicine5(6)870-90(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vps15-deficient mouse tissues are competent for LC3-positive autophagosome formation and maintain mTOR activation.
Title: Defects of Vps15 in skeletal muscles lead to autophagic vacuolar myopathy and lysosomal disease.

The following Pik3r4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pik3r4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18669	NM_001081309.1 Latest version!	Mus musculus phosphoinositide-3-kinase regulatory subunit 4 (Pik3r4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pik3r4 ( NM_001081309.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074778.1 Mus musculus phosphoinositide-3-kinase regulatory subunit 4 (Pik3r4), mRNA.

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CloneID	OMu18669
Clone ID Related Accession (Same CDS sequence)	NM_001081309.1
Accession Version	NM_001081309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-18
Organism	Mus musculus(house mouse)
Product	phosphoinositide 3-kinase regulatory subunit 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC157514.2. On Feb 6, 2007 this sequence version replaced XM_889806.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC157947.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAATC AGCTGGCTGG CATCGCTCCT TCCCAGATCC TGTCTGTAGA GAGTTATTTC 
TCAGACATCC ATGACTTTGA GTATGATAAG AGCCTGGGAA GCACTCGGTT TTTTAAAGTC 
GCTCGAGCCA AGCACCGGGA AGGCCTGGTG GTTGTGAAGG TCTTTGCAAT TCAGGATCCC 
ACGTTACCCT TAACTAGTTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTCCACTCC 
GCCCAGAACT GCCTCCCCTT CCAGAAAGCA GCAGAAAAGG CGTCTGAGAA AGCAGCCATG 
CTGTTCCGGC AGTATGTGAG AGACAACCTC TACGATCGCA TCAGTACCCG CCCGTTCCTA 
AACAACATCG AGAAGCGCTG GATCGCCTTC CAGATCCTGA CGGCCGTGGA CCAAGCGCAC 
AAATCTGGAG TCCGCCACGG AGACATCAAG ACAGAGAATG TGATGGTCAC CAGTTGGAAC 
TGGGTCCTCC TAACCGACTT CGCCAGTTTT AAGCCCACGT ATCTTCCCGA GGACAACCCA 
GCGGATTTCA ACTATTTCTT TGACACCTCG AGAAGGAGGA CATGCTACAT AGCTCCCGAG 
CGCTTCGTTG ACGGTGGGAT GTTCGCCACC GAGTTAGAGT ACATGAGAGA CCCCTCAACC 
CCGCTTGTCG ACCTGAATAG CAATCAGAGA GCGCGGGGAG AGCTGAAGAG AGCCATGGAC 
ATCTTTTCGG CAGGTTGCGT GATAGCTGAG CTCTTTACGG AAGGCGTACC TTTGTTTGAT 
CTCTCTCAGC TGCTGGCGTA TAGAAACGGA CATTTTTTCC CTGAACAAGT GCTAAACAAA 
ATTGAAGATC GCAGTATCAG AGACTTGGTA ACTCAGATGA TTAACCGTGA GCCCGAGAAG 
CGTTTGGAAG CTGAGGACTA CCTGAAGCAG CAGCGAGGCA ACGCCTTCCC TGAGATATTT 
TATACTTTCC TTCAGCCTTA CATGGCCCAG TTCGCCAAGG AAACCTTTCT CTCTGCAGAT 
GAGCGGATTT TGGTTATACG GAAAGATTTG GGCAACATTA TTCACAACCT CTGTGGACAT 
GATTTGCCAG AAAAAGCAGA AGGGGAGTCC AGGGCCAGTG GTCTGGTTGT CCTGGTGTCC 
GTGATAACGT CCTGCCTGCA GACACTGAAG TCCTGCGACT CCAAACTGGC CGCCTTGGAG 
CTCATCCTGC ATCTGGCCCC CAGACTGAGC GTCGAGATCC TTCTGGATCG CATCACTCCC 
TACCTGTTGC ATTTCAGCAA TGACTCTGTC CCAAGAGTGC GGGCTGAAGC CCTGAGGACA 
CTCACCAAAG TCCTTGCCCT TGTCCAAGAG GTTCCTCGCA ACGATGTCAA TATCTATCCA 
GAGTATATTC TCCCCGGCAT AGCCCACCTG GCCCAGGATG ACGCTACCAT CGTCAGACTG 
GCCTACGCTG AAAACATAGC TCTGTTGGCC GAGACGGCTC TGCGATTCCT GGAGCTTGTG 
CAGCTGAAAA CTCTCAACAT GGAGAACGAG CCCGATAACG AGGAGGTAGA TGAAGCCACG 
CGTCCTAACG GAGACTATGA CACAGAGCTC CAGGCCTTGC ATGAGATGGT CCAGCAGAAG 
GTGGTCACGT TGCTAAGCGA CCCTGAGAAT ATCGTGAAAC AGACGCTGAT GGAGAGTGGG 
ATCACACGCT TGTGCGTCTT CTTCGGACGC CAGAAGGCCA ACGACGTTCT CTTGTCCCAC 
ATGATCACTT TCCTCAATGA TAAGAATGAT TGGCACCTGC GTGGAGCTTT CTTTGATAGC 
ATAGTCGGTG TGGCTGCCTA CGTTGGCTGG CAGAGCTCCT CTATCCTCAA GCCCCTGCTC 
CAGCAAGGCC TCAGCGATGC TGAGGAGTTT GTCATCGTGA AAGCTCTCAA TGCCCTGACC 
TGCATGTGCC AGCTAGGGCT GCTGCAGAAA CCCCATGTCT ACGAGTTCGC CAGTGATATT 
GCTCCCTTCC TGTGTCACCC TAACCTGTGG ATACGCTATG GCGCTGTGGG ATTTATCACG 
GTGGTAGCTC ATCAGATCAG TACTGCTGAT GTCTACTGCA AGCTCATGCC GTATCTTGAC 
CCGTATATTA CACAGCCAGT GATACAGATT GAGAGGAAGC TGGTCCTGCT CAGTGTGCTA 
AAGGAGCCGG TGAGCCGCTC CATATTTGAC TATGCTCTGA GGTCGAAAGA CATCGCCAGC 
TTGTTCAGAC ATCTCCACAT GCGCCAGAAG AAGCGAAATG GCTCTCTTCT CGACTGCCCT 
CCACCTGAGG ACCCCACCAT AGCGCAACTT CTGAAGAAGT TGCTCTCACA GGGGATGACT 
GAAGAAGAGG AAGATAAACT TCTGGCACTG AAGGACTTCA TGATGAAATC AAATCGAGCC 
AAAGCTAATG CGGTAGACCA GAGCCACCTC CACGACAGCA GCCAGAAAGG TGTAATCGAC 
TTGGCCGCCT TAGGCATCAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAAGAACCA 
GATGAGAAAC GTGCTAGGAA ACATGTGAAA CAAGACTCCA ATGTAAATGA AGAGTGGAAA 
AGCATGTTTG GGTCATTGGA GCCACCGAAT ATCCCACAGG CCCTGCCTAA AACCAGTGAC 
CATGAGGTTG TTCAGCCTGG GAAGCCACCT CGCTCTGAGT CCTCTGCTGG TATCTGTGTC 
CCTTTGTCAA CGTCTCCACA GGTCTCAGAA GCAGCTCACA TCCCAAGTAA GAAGCCTGTG 
ATCCCGGTTG TGAGTAGCAC GGTCCTGCCG TCCACTTACC AGATCCGCAT CACCACGTGC 
AAGACTGAAC TTCAGCAGCT CATACAGCAG AAGAGGGAGC AGTGCAACGC GGAGCGCATA 
GCCAAGCAGA TGATGGAGAA TGCCGAGTGG GAGAGCAAGC CACCTCCACC TGGGTGGCGT 
CCTAAAGGGC TGCTAGTTGC ACATCTTCAT GAGCACAAAT CTGCTGTGAA TCGCATCAGA 
GTCTCTGATG AACACTTACT TTTTGCAACA TGTTCAAATG ATGGCACAGT GAAGATCTGG 
AACAGCCAGA AGATGGAGGG GAAGACCACC ACAACGCGGT CTATTCTCAC GTACAGCCGA 
ATTGGAGGAC GAGTCAAGAC GCTAACGTTT TGCCAAGGCT CCCACTACTT GGCCATAGCA 
TCTGATAATG GTGCTGTCCA GCTTCTTGGA ATTGAGGCGT CTAAGTTACC CAAGTCTCCT 
AAAATTCACC CTCTACAAAG CAGGATTCTG GATCAGAAGG AAGATGGCTG TGTGGTGGAC 
ATGCATCACT TCAACTCCGG GGCACAGTCC GTTCTTGCCT ATGCCACGGT GAATGGCTCT 
CTGGTTGGAT GGGATCTCAG GTCTTCAAGC AACGCGTGGA CATTAAAGCA TGACCTAAAG 
TCAGGCCTCA TCACTTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTGTG CATAGGCACG 
AGCAGCGGTG CCATGGCGTG TTGGGACATG AGGTTCCAGT TGCCCATCTC CAGTCACTGT 
CATCCCTCCA GAGCTCGCAT CCGGCGCCTC TCCATGCACC CCCTGTACCA GTCCTGGGTA 
ATCGCAGCTG TTCAGGGCAA CAATGAAGTC TCCATGTGGG ACATGGAAAC TGGCGACAGG 
AGACTGACTC TCTGGGCCAG CAGCGCACCG CCACTGTCTG AATTACAGCC TTCACCGCAC 
AGCGTCCATG GCATCTACTG CAGCCCTGCA GATGGAAACC CTATCCTGCT GACCGCTGGC 
TCAGACATGA AAATAAGGTT CTGGGACTTG GTTTCCCCAG AGAGGTCCTA TGTTGTTGCG 
GGAAGTACAG GTTCCCCGTC TGTCTCCTAC TACAAGAAGA TAATAGAAGG CACCGAGGTC 
GTCCAGGAAA TTCAGAATAA GCAGAAGGTT GGACCGAGTG ATGATACCCC TCGGAGGGGC 
CCGGAGTCTC TGCCTGTGGG ACATCATGAC ATCATCACAG ACATTGCCAC CTTCCAGACC 
ACTCAGGGCT TCATTGTGAC TGCTTCTAGA GACGGAATTG TGAAGGTGTG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074778.1
CDS	438..4514
Translation	MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQELEELKIRLHSAQNCLPFQKAAEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNSNQRARGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNKIEDRSIRDLVTQMINREPEKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLGNIIHNLCGHDLPEKAEGESRASGLVVLVSVITSCLQTLKSCDSKLAALELILHLAPRLSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVQEVPRNDVNIYPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKTLNMENEPDNEEVDEATRPNGDYDTELQALHEMVQQKVVTLLSDPENIVKQTLMESGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDAEEFVIVKALNALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVGFITVVAHQISTADVYCKLMPYLDPYITQPVIQIERKLVLLSVLKEPVSRSIFDYALRSKDIASLFRHLHMRQKKRNGSLLDCPPPEDPTIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSNRAKANAVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEPDEKRARKHVKQDSNVNEEWKSMFGSLEPPNIPQALPKTSDHEVVQPGKPPRSESSAGICVPLSTSPQVSEAAHIPSKKPVIPVVSSTVLPSTYQIRITTCKTELQQLIQQKREQCNAERIAKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHLLFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTFCQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITSFAVDIHQCWLCIGTSSGAMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWVIAAVQGNNEVSMWDMETGDRRLTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTAGSDMKIRFWDLVSPERSYVVAGSTGSPSVSYYKKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDIATFQTTQGFIVTASRDGIVKVWK

Target ORF information:

RefSeq Version	NM_001081309.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide-3-kinase regulatory subunit 4 (Pik3r4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081309.1

ORF Insert Sequence:

ATGGGAAATC AGCTGGCTGG CATCGCTCCT TCCCAGATCC TGTCTGTAGA GAGTTATTTC 
TCAGACATCC ATGACTTTGA GTATGATAAG AGCCTGGGAA GCACTCGGTT TTTTAAAGTC 
GCTCGAGCCA AGCACCGGGA AGGCCTGGTG GTTGTGAAGG TCTTTGCAAT TCAGGATCCC 
ACGTTACCCT TAACTAGTTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTCCACTCC 
GCCCAGAACT GCCTCCCCTT CCAGAAAGCA GCAGAAAAGG CGTCTGAGAA AGCAGCCATG 
CTGTTCCGGC AGTATGTGAG AGACAACCTC TACGATCGCA TCAGTACCCG CCCGTTCCTA 
AACAACATCG AGAAGCGCTG GATCGCCTTC CAGATCCTGA CGGCCGTGGA CCAAGCGCAC 
AAATCTGGAG TCCGCCACGG AGACATCAAG ACAGAGAATG TGATGGTCAC CAGTTGGAAC 
TGGGTCCTCC TAACCGACTT CGCCAGTTTT AAGCCCACGT ATCTTCCCGA GGACAACCCA 
GCGGATTTCA ACTATTTCTT TGACACCTCG AGAAGGAGGA CATGCTACAT AGCTCCCGAG 
CGCTTCGTTG ACGGTGGGAT GTTCGCCACC GAGTTAGAGT ACATGAGAGA CCCCTCAACC 
CCGCTTGTCG ACCTGAATAG CAATCAGAGA GCGCGGGGAG AGCTGAAGAG AGCCATGGAC 
ATCTTTTCGG CAGGTTGCGT GATAGCTGAG CTCTTTACGG AAGGCGTACC TTTGTTTGAT 
CTCTCTCAGC TGCTGGCGTA TAGAAACGGA CATTTTTTCC CTGAACAAGT GCTAAACAAA 
ATTGAAGATC GCAGTATCAG AGACTTGGTA ACTCAGATGA TTAACCGTGA GCCCGAGAAG 
CGTTTGGAAG CTGAGGACTA CCTGAAGCAG CAGCGAGGCA ACGCCTTCCC TGAGATATTT 
TATACTTTCC TTCAGCCTTA CATGGCCCAG TTCGCCAAGG AAACCTTTCT CTCTGCAGAT 
GAGCGGATTT TGGTTATACG GAAAGATTTG GGCAACATTA TTCACAACCT CTGTGGACAT 
GATTTGCCAG AAAAAGCAGA AGGGGAGTCC AGGGCCAGTG GTCTGGTTGT CCTGGTGTCC 
GTGATAACGT CCTGCCTGCA GACACTGAAG TCCTGCGACT CCAAACTGGC CGCCTTGGAG 
CTCATCCTGC ATCTGGCCCC CAGACTGAGC GTCGAGATCC TTCTGGATCG CATCACTCCC 
TACCTGTTGC ATTTCAGCAA TGACTCTGTC CCAAGAGTGC GGGCTGAAGC CCTGAGGACA 
CTCACCAAAG TCCTTGCCCT TGTCCAAGAG GTTCCTCGCA ACGATGTCAA TATCTATCCA 
GAGTATATTC TCCCCGGCAT AGCCCACCTG GCCCAGGATG ACGCTACCAT CGTCAGACTG 
GCCTACGCTG AAAACATAGC TCTGTTGGCC GAGACGGCTC TGCGATTCCT GGAGCTTGTG 
CAGCTGAAAA CTCTCAACAT GGAGAACGAG CCCGATAACG AGGAGGTAGA TGAAGCCACG 
CGTCCTAACG GAGACTATGA CACAGAGCTC CAGGCCTTGC ATGAGATGGT CCAGCAGAAG 
GTGGTCACGT TGCTAAGCGA CCCTGAGAAT ATCGTGAAAC AGACGCTGAT GGAGAGTGGG 
ATCACACGCT TGTGCGTCTT CTTCGGACGC CAGAAGGCCA ACGACGTTCT CTTGTCCCAC 
ATGATCACTT TCCTCAATGA TAAGAATGAT TGGCACCTGC GTGGAGCTTT CTTTGATAGC 
ATAGTCGGTG TGGCTGCCTA CGTTGGCTGG CAGAGCTCCT CTATCCTCAA GCCCCTGCTC 
CAGCAAGGCC TCAGCGATGC TGAGGAGTTT GTCATCGTGA AAGCTCTCAA TGCCCTGACC 
TGCATGTGCC AGCTAGGGCT GCTGCAGAAA CCCCATGTCT ACGAGTTCGC CAGTGATATT 
GCTCCCTTCC TGTGTCACCC TAACCTGTGG ATACGCTATG GCGCTGTGGG ATTTATCACG 
GTGGTAGCTC ATCAGATCAG TACTGCTGAT GTCTACTGCA AGCTCATGCC GTATCTTGAC 
CCGTATATTA CACAGCCAGT GATACAGATT GAGAGGAAGC TGGTCCTGCT CAGTGTGCTA 
AAGGAGCCGG TGAGCCGCTC CATATTTGAC TATGCTCTGA GGTCGAAAGA CATCGCCAGC 
TTGTTCAGAC ATCTCCACAT GCGCCAGAAG AAGCGAAATG GCTCTCTTCT CGACTGCCCT 
CCACCTGAGG ACCCCACCAT AGCGCAACTT CTGAAGAAGT TGCTCTCACA GGGGATGACT 
GAAGAAGAGG AAGATAAACT TCTGGCACTG AAGGACTTCA TGATGAAATC AAATCGAGCC 
AAAGCTAATG CGGTAGACCA GAGCCACCTC CACGACAGCA GCCAGAAAGG TGTAATCGAC 
TTGGCCGCCT TAGGCATCAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAAGAACCA 
GATGAGAAAC GTGCTAGGAA ACATGTGAAA CAAGACTCCA ATGTAAATGA AGAGTGGAAA 
AGCATGTTTG GGTCATTGGA GCCACCGAAT ATCCCACAGG CCCTGCCTAA AACCAGTGAC 
CATGAGGTTG TTCAGCCTGG GAAGCCACCT CGCTCTGAGT CCTCTGCTGG TATCTGTGTC 
CCTTTGTCAA CGTCTCCACA GGTCTCAGAA GCAGCTCACA TCCCAAGTAA GAAGCCTGTG 
ATCCCGGTTG TGAGTAGCAC GGTCCTGCCG TCCACTTACC AGATCCGCAT CACCACGTGC 
AAGACTGAAC TTCAGCAGCT CATACAGCAG AAGAGGGAGC AGTGCAACGC GGAGCGCATA 
GCCAAGCAGA TGATGGAGAA TGCCGAGTGG GAGAGCAAGC CACCTCCACC TGGGTGGCGT 
CCTAAAGGGC TGCTAGTTGC ACATCTTCAT GAGCACAAAT CTGCTGTGAA TCGCATCAGA 
GTCTCTGATG AACACTTACT TTTTGCAACA TGTTCAAATG ATGGCACAGT GAAGATCTGG 
AACAGCCAGA AGATGGAGGG GAAGACCACC ACAACGCGGT CTATTCTCAC GTACAGCCGA 
ATTGGAGGAC GAGTCAAGAC GCTAACGTTT TGCCAAGGCT CCCACTACTT GGCCATAGCA 
TCTGATAATG GTGCTGTCCA GCTTCTTGGA ATTGAGGCGT CTAAGTTACC CAAGTCTCCT 
AAAATTCACC CTCTACAAAG CAGGATTCTG GATCAGAAGG AAGATGGCTG TGTGGTGGAC 
ATGCATCACT TCAACTCCGG GGCACAGTCC GTTCTTGCCT ATGCCACGGT GAATGGCTCT 
CTGGTTGGAT GGGATCTCAG GTCTTCAAGC AACGCGTGGA CATTAAAGCA TGACCTAAAG 
TCAGGCCTCA TCACTTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTGTG CATAGGCACG 
AGCAGCGGTG CCATGGCGTG TTGGGACATG AGGTTCCAGT TGCCCATCTC CAGTCACTGT 
CATCCCTCCA GAGCTCGCAT CCGGCGCCTC TCCATGCACC CCCTGTACCA GTCCTGGGTA 
ATCGCAGCTG TTCAGGGCAA CAATGAAGTC TCCATGTGGG ACATGGAAAC TGGCGACAGG 
AGACTGACTC TCTGGGCCAG CAGCGCACCG CCACTGTCTG AATTACAGCC TTCACCGCAC 
AGCGTCCATG GCATCTACTG CAGCCCTGCA GATGGAAACC CTATCCTGCT GACCGCTGGC 
TCAGACATGA AAATAAGGTT CTGGGACTTG GTTTCCCCAG AGAGGTCCTA TGTTGTTGCG 
GGAAGTACAG GTTCCCCGTC TGTCTCCTAC TACAAGAAGA TAATAGAAGG CACCGAGGTC 
GTCCAGGAAA TTCAGAATAA GCAGAAGGTT GGACCGAGTG ATGATACCCC TCGGAGGGGC 
CCGGAGTCTC TGCCTGTGGG ACATCATGAC ATCATCACAG ACATTGCCAC CTTCCAGACC 
ACTCAGGGCT TCATTGTGAC TGCTTCTAGA GACGGAATTG TGAAGGTGTG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.
Nature neuroscience(2018 Jan)
Gstrein T,Edwards A,P?istoupilov? A,Leca I,Breuss M,Pilat-Carotta S,Hansen AH,Tripathy R,Traunbauer AK,Hochstoeger T,Rosoklija G,Repic M,Landler L,Str?neck? V,D?rnberger G,Keane TM,Zuber J,Adams DJ,Flint J,Honzik T,Gut M,Beltran S,Mechtler K,Sherr E,Kmoch S,Gut I,Keays DA

Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling.
Nature communications68283(2015 Sep)
Nemazanyy I,Montagnac G,Russell RC,Morzyglod L,Burnol AF,Guan KL,Pende M,Panasyuk G

Connexins modulate autophagosome biogenesis.
Nature cell biology16(5)401-14(2014 May)
Bejarano E,Yuste A,Patel B,Stout RF Jr,Spray DC,Cuervo AM

Functional interaction between autophagy and ciliogenesis.
Nature502(7470)194-200(2013 Oct)
Pampliega O,Orhon I,Patel B,Sridhar S,D?az-Carretero A,Beau I,Codogno P,Satir BH,Satir P,Cuervo AM

Defects of Vps15 in skeletal muscles lead to autophagic vacuolar myopathy and lysosomal disease.
EMBO molecular medicine5(6)870-90(2013 Jun)
Nemazanyy I,Blaauw B,Paolini C,Caillaud C,Protasi F,Mueller A,Proikas-Cezanne T,Russell RC,Guan KL,Nishino I,Sandri M,Pende M,Panasyuk G

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Pik3r4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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