Kdm5b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kdm5b
Entrez Gene ID	75605
Full Name	lysine (K)-specific demethylase 5B
Synonyms	2010009J12Rik,2210016I17Rik,AW556288,D1Ertd202e,Jarid1b,PLU-1,PUT1,Plu1,RBBP2H1A,Rb-Bp2,mKIAA4034
General protein information	Preferred Names lysine (K)-specific demethylase 5B Names lysine-specific demethylase 5B histone demethylase JARID1B jumonji, AT rich interactive domain 1B (Rbp2 like) jumonji/ARID domain-containing protein 1B putative DNA/chromatin binding motif 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 E4\|1 58.24 cM
Summary	This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Homozygous mutant mice display decreased body weight, decreased female fertility, lower uterine weight, and a delay in mammary development. Knockout of this gene has also been associated with embryonic lethality. [provided by RefSeq, Dec 2016].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017312616.1	XP_017168105.1	lysine-specific demethylase 5B isoform X1
XM_011248090.2	XP_011246392.1	lysine-specific demethylase 5B isoform X1
XM_011248090.3	XP_011246392.1	lysine-specific demethylase 5B isoform X1
XM_017312617.2	XP_017168106.1	lysine-specific demethylase 5B isoform X2
XM_017312617.1	XP_017168106.1	lysine-specific demethylase 5B isoform X2
NM_152895.2	NP_690855.2	lysine-specific demethylase 5B
XM_017312616.2	XP_017168105.1	lysine-specific demethylase 5B isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KDM5B	XP_002694248.2
Rattus norvegicus (Norway rat)	Kdm5b	NP_001100647.1
Canis lupus familiaris (dog)	KDM5B	XP_005622266.1
Gallus gallus (chicken)	KDM5B	NP_001026200.1
Danio rerio (zebrafish)	kdm5bb	NP_001002166.1
Xenopus tropicalis (tropical clawed frog)	kdm5b	XP_004910700.1
Mus musculus (house mouse)	Kdm5b	NP_690855.2
Caenorhabditis elegans (roundworm)	rbr-2	NP_502032.2
Pan troglodytes (chimpanzee)	KDM5B	XP_514104.3
Homo sapiens (human)	KDM5B	NP_006609.3
	LOC706437	XP_002808303.1

Gene Ontology
Bibliography

Related articles in PubMed

Histone demethylase jumonji AT-rich interactive domain 1B (JARID1B) controls mammary gland development by regulating key developmental and lineage specification genes.
Zou MR, Cao J, Liu Z, Huh SJ, Polyak K, Yan Q
The Journal of biological chemistry289(25)17620-33(2014 Jun)

The histone demethylase Jarid1b ensures faithful mouse development by protecting developmental genes from aberrant H3K4me3.
Albert M, Schmitz SU, Kooistra SM, Malatesta M, Morales Torres C, Rekling JC, Johansen JV, Abarrategui I, Helin K
PLoS genetics9(4)e1003461(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KDM5B, which demethylates H3K4me3, plays an integral role in regulating RNAPII occupancy, transcriptional initiation and elongation, and alternative splicing events in embryonic stem cells.
Title: H3K4 demethylase KDM5B regulates global dynamics of transcription elongation and alternative splicing in embryonic stem cells.

data suggest Kdm5b negatively regulates neurogenesis and represses reelin in neural stem cells from the adult subventricular zone.
Title: Inhibition of the histone demethylase Kdm5b promotes neurogenesis and derepresses Reln (reelin) in neural stem cells from the adult subventricular zone of mice.

findings demonstrate that dissociation of the GAPDH/Siah1 pro-apoptotic complex can block high glucose-induced pericyte apoptosis, widely considered a hallmark feature of DR
Title: Epigenetic Control of the Bone-master Runx2 Gene during Osteoblast-lineage Commitment by the Histone Demethylase JARID1B/KDM5B.

These results suggest that KDM5B acts to repress type I IFN and other innate cytokines to promote an altered immune response following Respiratory syncytial virus infection that contributes to development of chronic disease.
Title: RSV-Induced H3K4 Demethylase KDM5B Leads to Regulation of Dendritic Cell-Derived Innate Cytokines and Exacerbates Pathogenesis In Vivo.

Deletion of Jarid1b compromises hematopoietic stem cell (HSC) self-renewal capacity; Jarid1b is a positive regulator of HSC potential.
Title: The histone demethylase Jarid1b is required for hematopoietic stem cell self-renewal in mice.

The following Kdm5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kdm5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu71360	XM_011248090.3 Latest version!	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu71360	XM_017312616.2 Latest version!	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu77308	XM_017312617.2 Latest version!	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu21127	NM_152895.2 Latest version!	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$909.30 $1299.00
View Old Accession Versions >>
OMu71360	XM_011248090.2	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OMu77308	XM_017312617.1	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu71360	XM_017312616.1	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kdm5b ( XM_017312616.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168105.1 Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X1, mRNA.

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CloneID	OMu71360
Clone ID Related Accession (Same CDS sequence)	XM_017312616.1 , XM_011248090.2 , XM_011248090.3 , XM_017312616.2
Accession Version	XM_017312616.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCACCA GGTCTGTGTT 
GGTGTCTCTC CAGAGATGGC AGAGAAGGAA GACTACATCT GTGTGCGCTG TACTGGGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168105.1
CDS	1131..5291
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKPHDIPQRQSVQPAETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCTTPKWENEKEMKSTIKQEPTEKKDCELESEKEKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLVPPLHDVPKGDWRCPKCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQQSQKLLSEEMPSAAELQELLDVSFEFDVELPQLTEMRIRLEQARWLEEVQQACLDSSSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLRARPRHSLSSLATAVKEMEEIPAYLPNGTVLKDSVQRARDWVQDVDALQAGGRVPVLETLIELVARGRSIPVHLNSLPRLEMLVAEVHAWKECAAKTFLPENSTYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPSGKKRSTKLESLSDLERALMESKETAAAMATLGEARLREMEALQSLRFANEEKLLSPVQDLEMKVCLCQKTPATPMIQCELCRDAFHTSCVAAPSISQSSRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKLVQDQVGSGLLSSRWPASAGQASATDKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGQSCLPLHGLSPEVNELLMEAQLLQVSLPEIQELYQTLLTKPSSVQQADRSSPVRSSSEKNDCLRGKRDAINSPERKLKRRPEREGLPSERWDRVKHMRTPQKKKIKLSHPKDMDSFKLERERSYDLVRNAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTGKDAPSRK

Target ORF information:

RefSeq Version	XM_017312616.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312616.1

ORF Insert Sequence:

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCACCA GGTCTGTGTT 
GGTGTCTCTC CAGAGATGGC AGAGAAGGAA GACTACATCT GTGTGCGCTG TACTGGGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm5b ( XM_011248090.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246392.1 Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X2, mRNA.

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CloneID	OMu71360
Clone ID Related Accession (Same CDS sequence)	XM_017312616.1 , XM_011248090.2 , XM_011248090.3 , XM_017312616.2
Accession Version	XM_011248090.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCACCA GGTCTGTGTT 
GGTGTCTCTC CAGAGATGGC AGAGAAGGAA GACTACATCT GTGTGCGCTG TACTGGGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246392.1
CDS	1376..5536
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKPHDIPQRQSVQPAETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCTTPKWENEKEMKSTIKQEPTEKKDCELESEKEKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLVPPLHDVPKGDWRCPKCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQQSQKLLSEEMPSAAELQELLDVSFEFDVELPQLTEMRIRLEQARWLEEVQQACLDSSSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLRARPRHSLSSLATAVKEMEEIPAYLPNGTVLKDSVQRARDWVQDVDALQAGGRVPVLETLIELVARGRSIPVHLNSLPRLEMLVAEVHAWKECAAKTFLPENSTYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPSGKKRSTKLESLSDLERALMESKETAAAMATLGEARLREMEALQSLRFANEEKLLSPVQDLEMKVCLCQKTPATPMIQCELCRDAFHTSCVAAPSISQSSRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKLVQDQVGSGLLSSRWPASAGQASATDKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGQSCLPLHGLSPEVNELLMEAQLLQVSLPEIQELYQTLLTKPSSVQQADRSSPVRSSSEKNDCLRGKRDAINSPERKLKRRPEREGLPSERWDRVKHMRTPQKKKIKLSHPKDMDSFKLERERSYDLVRNAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTGKDAPSRK

Target ORF information:

RefSeq Version	XM_011248090.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248090.2

ORF Insert Sequence:

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCACCA GGTCTGTGTT 
GGTGTCTCTC CAGAGATGGC AGAGAAGGAA GACTACATCT GTGTGCGCTG TACTGGGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm5b ( XM_011248090.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246392.1 Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu71360
Clone ID Related Accession (Same CDS sequence)	XM_017312616.1 , XM_011248090.2 , XM_011248090.3 , XM_017312616.2
Accession Version	XM_011248090.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCACCA GGTCTGTGTT 
GGTGTCTCTC CAGAGATGGC AGAGAAGGAA GACTACATCT GTGTGCGCTG TACTGGGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246392.1
CDS	455..4615
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKPHDIPQRQSVQPAETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCTTPKWENEKEMKSTIKQEPTEKKDCELESEKEKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLVPPLHDVPKGDWRCPKCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQQSQKLLSEEMPSAAELQELLDVSFEFDVELPQLTEMRIRLEQARWLEEVQQACLDSSSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLRARPRHSLSSLATAVKEMEEIPAYLPNGTVLKDSVQRARDWVQDVDALQAGGRVPVLETLIELVARGRSIPVHLNSLPRLEMLVAEVHAWKECAAKTFLPENSTYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPSGKKRSTKLESLSDLERALMESKETAAAMATLGEARLREMEALQSLRFANEEKLLSPVQDLEMKVCLCQKTPATPMIQCELCRDAFHTSCVAAPSISQSSRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKLVQDQVGSGLLSSRWPASAGQASATDKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGQSCLPLHGLSPEVNELLMEAQLLQVSLPEIQELYQTLLTKPSSVQQADRSSPVRSSSEKNDCLRGKRDAINSPERKLKRRPEREGLPSERWDRVKHMRTPQKKKIKLSHPKDMDSFKLERERSYDLVRNAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTGKDAPSRK

Target ORF information:

RefSeq Version	XM_011248090.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248090.3

ORF Insert Sequence:

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCACCA GGTCTGTGTT 
GGTGTCTCTC CAGAGATGGC AGAGAAGGAA GACTACATCT GTGTGCGCTG TACTGGGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm5b ( XM_017312616.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168105.1 Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X2, mRNA.

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CloneID	OMu71360
Clone ID Related Accession (Same CDS sequence)	XM_017312616.1 , XM_011248090.2 , XM_011248090.3 , XM_017312616.2
Accession Version	XM_017312616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312616.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCACCA GGTCTGTGTT 
GGTGTCTCTC CAGAGATGGC AGAGAAGGAA GACTACATCT GTGTGCGCTG TACTGGGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168105.1
CDS	1131..5291
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKPHDIPQRQSVQPAETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCTTPKWENEKEMKSTIKQEPTEKKDCELESEKEKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLVPPLHDVPKGDWRCPKCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQQSQKLLSEEMPSAAELQELLDVSFEFDVELPQLTEMRIRLEQARWLEEVQQACLDSSSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLRARPRHSLSSLATAVKEMEEIPAYLPNGTVLKDSVQRARDWVQDVDALQAGGRVPVLETLIELVARGRSIPVHLNSLPRLEMLVAEVHAWKECAAKTFLPENSTYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPSGKKRSTKLESLSDLERALMESKETAAAMATLGEARLREMEALQSLRFANEEKLLSPVQDLEMKVCLCQKTPATPMIQCELCRDAFHTSCVAAPSISQSSRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKLVQDQVGSGLLSSRWPASAGQASATDKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGQSCLPLHGLSPEVNELLMEAQLLQVSLPEIQELYQTLLTKPSSVQQADRSSPVRSSSEKNDCLRGKRDAINSPERKLKRRPEREGLPSERWDRVKHMRTPQKKKIKLSHPKDMDSFKLERERSYDLVRNAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTGKDAPSRK

Target ORF information:

RefSeq Version	XM_017312616.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312616.2

ORF Insert Sequence:

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCACCA GGTCTGTGTT 
GGTGTCTCTC CAGAGATGGC AGAGAAGGAA GACTACATCT GTGTGCGCTG TACTGGGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm5b ( XM_017312617.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168106.1 Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X3, mRNA.

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CloneID	OMu77308
Clone ID Related Accession (Same CDS sequence)	XM_017312617.2 , XM_017312617.1
Accession Version	XM_017312617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTCAGTG AGGTTTGGGC CATAGAGGAC GCGCTTTCCC CTAACTCAGA AACCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168106.1
CDS	319..4398
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKPHDIPQRQSVQPAETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCTTPKWENEKEMKSTIKQEPTEKKDCELESEKEKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLVPPLHDVPKGDWRCPKCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQQSQKLLSEEMPSAAELQELLDVSFEFDVELPQLTEMRIRLEQARWLEEVQQACLDSSSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLRARPRHSLSSLATAVKEMEEIPAYLPNGTVLKDSVQRARDWVQDVDALQAGGRVPVLETLIELVARGRSIPVHLNSLPRLEMLVAEVHAWKECAAKTFLPENSTYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPSGKKRSTKLESLSDLERALMESKETAAAMATLGEARLREMEALQSLRFANEEKLLSPVQDLEMKVCLCQKTPATPMIQCELCRDAFHTSCVAAPSISQSSRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKLVQDQVGSGLLSSRWPASAGQASATDKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGQSCLPLHGLSPEVNELLMEAQLLQVSLPEIQELYQTLLTKPSSVQQADRSSPVRSSSEKNDCLRGKRDAINSPERKLKRRPEREGLPSERWDRVKHMRTPQKKKIKLSHPKDMDSFKLERERSYDLVRNAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVSEVWAIEDALSPNSETL

Target ORF information:

RefSeq Version	XM_017312617.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312617.2

ORF Insert Sequence:

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTCAGTG AGGTTTGGGC CATAGAGGAC GCGCTTTCCC CTAACTCAGA AACCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm5b ( XM_017312617.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168106.1 Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu77308
Clone ID Related Accession (Same CDS sequence)	XM_017312617.2 , XM_017312617.1
Accession Version	XM_017312617.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTCAGTG AGGTTTGGGC CATAGAGGAC GCGCTTTCCC CTAACTCAGA AACCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168106.1
CDS	319..4398
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKPHDIPQRQSVQPAETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCTTPKWENEKEMKSTIKQEPTEKKDCELESEKEKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLVPPLHDVPKGDWRCPKCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQQSQKLLSEEMPSAAELQELLDVSFEFDVELPQLTEMRIRLEQARWLEEVQQACLDSSSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLRARPRHSLSSLATAVKEMEEIPAYLPNGTVLKDSVQRARDWVQDVDALQAGGRVPVLETLIELVARGRSIPVHLNSLPRLEMLVAEVHAWKECAAKTFLPENSTYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPSGKKRSTKLESLSDLERALMESKETAAAMATLGEARLREMEALQSLRFANEEKLLSPVQDLEMKVCLCQKTPATPMIQCELCRDAFHTSCVAAPSISQSSRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKLVQDQVGSGLLSSRWPASAGQASATDKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGQSCLPLHGLSPEVNELLMEAQLLQVSLPEIQELYQTLLTKPSSVQQADRSSPVRSSSEKNDCLRGKRDAINSPERKLKRRPEREGLPSERWDRVKHMRTPQKKKIKLSHPKDMDSFKLERERSYDLVRNAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVSEVWAIEDALSPNSETL

Target ORF information:

RefSeq Version	XM_017312617.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312617.1

ORF Insert Sequence:

ATGGGTTTTG CTCCTGGTAA AGCTGTGGGC TCACATATCA GGGGACATTA TGAGCGAATA 
CTCAATCCAT ACAATTTGTT CCTGTCTGGA GACAGTCTGA GGTGTCTTCA GAAGCCTAAC 
CTGACCTCCG ACACAAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCCG CAGAAACCTG TCCTCCGGCC CGCCGAGCAA AGCGCATGAG AGCAGAGGCT 
ATGAATATTA AAATTGAGCC AGAAGAGGCA ACAGAAGCCA GGACTCATAA TCTGAGACGC 
CGAATGGGTT GCACAACTCC AAAATGGGAA AATGAGAAAG AAATGAAAAG CACCATCAAG 
CAAGAGCCCA CTGAGAAGAA AGACTGCGAG TTAGAAAGTG AGAAGGAGAA GCCCAAGAGC 
CGGGCCAAGA AAACGGCCAC TGCGGTGGAC CTGTATGTGT GCCTCTTATG TGGAAGTGGC 
AATGACGAGG ACCGACTCCT ACTGTGTGAC GGCTGTGATG ACAGTTACCA CACCTTCTGC 
CTGGTTCCCC CTCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAAGAATGTA ATAAGCCACA AGAAGCTTTT GGTTTTGAAC AAGCAGCAAG GGACTATACA 
CTTCGTACAT TTGGAGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTTC CCACAGAGCT GGTGGAGAAG GAGTTTTGGC GGCTCGTAAG CACTATTGAA 
GAAGACGTCA CGGTGGAGTA TGGAGCTGAT ATTGCTTCAA AAGAGTTTGG CAGTGGCTTT 
CCTGTTCGAG ATGGGAAAAT CAAGATTTCT CCTGAGGAAG AGGAATACCT GGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCTCATAT TACTGCTGAT 
ATATGTGGCA TGAAACTCCC CTGGTTGTAT GTGGGCATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATC AACTACCTAC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TTCCAGGCTA CGCTGCTGAG CAGCTAGAAA ACGTGATGAA GAAGCTCGCT 
CCTGAGCTCT TTGTGTCCCA GCCGGACCTG CTCCACCAGC TGGTGACCAT CATGAACCCC 
AACACTCTGA TGACTCATGA AGTGCCTGTT TATCGAACGA ATCAGTGTGC TGGGGAGTTT 
GTAATTACGT TTCCAAGAGC CTACCACAGT GGCTTCAACC AAGGTTTTAA TTTTGCCGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTGGGCC GCCAGTGTGT GGAGCATTAT 
CGCTTGCTGC ACCGTTATTG TGTCTTTTCC CACGATGAAA TGATATGCAA AATGGCTTCT 
AAGGCTGATG TATTAGACGT TGTGGTAGCG TCAACTGTTC AGAAGGACAT GGCCATCATG 
ATCGAGGACG AGAAAGCTTT AAGGGAAACT GTTCGGAAAT TGGGAGTAAT TGATTCTGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAGCGGCAGT GTATAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGCTCTTGT AAACCTGGCC TACTTGTTTG CCTACATCAT 
GTGAAAGAAT TGTGTTCTTG CCCCCCGTAT AAGTATAACC TGCGGTATAG ATACACTCTG 
GATGATCTCT ATCCAATGAT GAATGCATTA AAGCTTCGAG CAGAGTCTTA CAATGAATGG 
GCCTTAAATG TGAATGAAGC CTTGGAAGCA AAGATCAATA AGAAGAAAAG TCTTGTCAGC 
TTTAAAGCTT TGATTGAAGA ATCAGAAATG AAAAAATTCC CAGACAATGA TCTTTTGCGT 
CACCTTCGAC TAGTCACGCA GGATGCAGAA AAGTGTGCCT CTGTTGCCCA GCAGTTGCTT 
AATGGCAAAA GGCAAACAAG GTATCGATCT GGTGGAGGAA AGTCTCAGAA TCAGCTGACG 
GTGAATGAAC TTAGACAGTT TGTGACTCAG CTGTATGCTC TGCCGTGCGT CCTTAGTCAG 
ACACCGTTGC TAAAGGATCT CTTGAATCGT GTGGAAGATT TCCAACAGCA AAGCCAAAAA 
CTACTGTCTG AGGAAATGCC TAGCGCTGCC GAGCTACAGG AGTTGCTGGA TGTCAGCTTT 
GAATTTGATG TTGAGCTTCC ACAGCTCACT GAGATGCGCA TTCGCTTAGA GCAGGCCCGC 
TGGCTGGAAG AGGTTCAGCA GGCTTGCCTG GACTCCAGCT CCCTTTCTTT GGATGATATG 
AGACGACTCA TAGACTTAGG GGTGGGGCTG GCTCCATATT CTGCTGTGGA GAAAGCTATG 
GCCCGCCTGC AGGAGCTGCT AACAGTGTCA GAACACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAGAGCAA GACCACGGCA TTCTTTGAGT AGCCTTGCTA CAGCAGTCAA AGAGATGGAG 
GAGATCCCCG CCTACCTGCC CAATGGTACA GTCCTGAAGG ATTCTGTGCA AAGAGCCAGA 
GACTGGGTTC AGGATGTGGA TGCATTGCAG GCAGGAGGAC GTGTGCCAGT GTTAGAGACA 
CTCATTGAAC TTGTTGCAAG AGGCCGATCT ATCCCAGTAC ATCTCAATTC TTTACCAAGA 
TTGGAAATGT TAGTAGCTGA AGTTCATGCA TGGAAAGAAT GTGCTGCTAA AACATTCTTG 
CCTGAGAATT CTACATATTC TCTGTTAGAG GTTCTTTGCC CTCGGTGTGA CATTGGCCTT 
TTGGGGCTGA AAAGGAAACA GAGAAAGTTG AAGGAGCCCT TGCCGAGTGG AAAGAAGAGA 
AGCACCAAGT TAGAGAGCCT GAGCGATCTG GAGAGGGCTC TGATGGAGAG CAAAGAGACT 
GCGGCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTACGAG AGATGGAAGC TTTGCAGTCT 
CTCAGATTTG CCAATGAAGA GAAATTGCTG TCACCTGTCC AAGACTTAGA GATGAAAGTC 
TGCTTGTGTC AGAAGACTCC AGCCACTCCC ATGATTCAGT GTGAACTCTG CAGGGATGCT 
TTCCATACTA GTTGTGTGGC AGCACCCAGT ATTTCACAAA GCTCCCGAAT CTGGCTTTGT 
CCTCACTGTC GGAGGTCAGA GAAGCCTCCA TTAGAGAAAA TTCTGCCTCT GCTGGCCTCC 
CTACAGCGTA TACGAGTTCG TCTTCCTGAA GGAGATGCAC TCCGATACAT GATTGAAAGA 
ACCGTGAACT GGCAACATAG AGCCCAGCAG CTGCTTTCTT CAGGGAATCT AAAACTTGTG 
CAAGACCAAG TGGGTTCTGG ACTGTTGTCT AGCAGATGGC CAGCCTCAGC AGGACAGGCA 
TCAGCAACAG ACAAGGTGTC TCAGCCTCCT GGCACAACAT CCTTTTCATT GCCCGATGAC 
TGGGACAACA GAACCTCATA TTTACACTCT CCCTTCTCTA CTGGACAGAG TTGTCTCCCC 
CTCCATGGTT TGAGTCCAGA AGTGAATGAA TTGCTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA GCTTTATCAG ACTCTACTTA CTAAGCCAAG CTCTGTTCAG 
CAAGCTGACC GAAGTTCACC AGTTAGATCC AGCAGTGAGA AGAACGATTG CCTTCGAGGG 
AAACGAGATG CAATCAACAG TCCTGAGAGG AAACTGAAAA GGCGCCCAGA AAGAGAAGGC 
CTTCCTAGCG AGCGGTGGGA TCGAGTTAAA CACATGCGGA CCCCCCAAAA GAAGAAAATC 
AAACTGAGTC ACCCCAAGGA CATGGACAGT TTCAAGTTAG AAAGAGAGCG TAGCTATGAC 
TTAGTGCGTA ATGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTACTC CGAACAGGAG 
GACTCTGAGG ATGAAGATGC CATCTGTCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTCAGTG AGGTTTGGGC CATAGAGGAC GCGCTTTCCC CTAACTCAGA AACCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm5b ( NM_152895.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_690855.2 Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), mRNA.

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CloneID	OMu21127
Clone ID Related Accession (Same CDS sequence)	NM_152895.2
Accession Version	NM_152895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 5B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from W35764.1, BC048180.1, CX567343.1, CX206086.1 and AK076888.1. On Nov 15, 2007 this sequence version replaced NM_152895.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY082429.1, AY082430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCGG CCACCACGCT GCCCCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GAGAGTTCCT GCCTCCCCCC GAGTGTCCGG TCTTCGAGCC GAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGG 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TCGCTTGTGA TGTCGATAAA 
CTTCATTTCA CCCCGCGGAT CCAGAGGCTG AATGAGCTGG AGGCCCAAAC ACGTGTAAAA 
TTGAATTTCC TGGACCAGAT TGCAAAGTAT TGGGAGCTAC AAGGAAGTAC TCTGAAGATT 
CCCCACGTGG AGAGAAAGAT CTTAGACTTG TTCCAGCTAA ATAAGTTAGT CGCAGAAGAA 
GGTGGGTTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCCAC CAAGATGGGT 
TTTGCTCCTG GTAAAGCTGT GGGCTCACAT ATCAGGGGAC ATTATGAGCG AATACTCAAT 
CCATACAATT TGTTCCTGTC TGGAGACAGT CTGAGGTGTC TTCAGAAGCC TAACCTGACC 
TCCGACACAA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCCGCAGAAA CCTGTCCTCC GGCCCGCCGA GCAAAGCGCA TGAGAGCAGA GGCTATGAAT 
ATTAAAATTG AGCCAGAAGA GGCAACAGAA GCCAGGACTC ATAATCTGAG ACGCCGAATG 
GGTTGCACAA CTCCAAAATG GGAAAATGAG AAAGAAATGA AAAGCACCAT CAAGCAAGAG 
CCCACTGAGA AGAAAGACTG CGAGTTAGAA AGTGAGAAGG AGAAGCCCAA GAGCCGGGCC 
AAGAAAACGG CCACTGCGGT GGACCTGTAT GTGTGCCTCT TATGTGGAAG TGGCAATGAC 
GAGGACCGAC TCCTACTGTG TGACGGCTGT GATGACAGTT ACCACACCTT CTGCCTGGTT 
CCCCCTCTCC ATGACGTTCC CAAGGGAGAC TGGAGGTGTC CTAAGTGTTT GGCTCAAGAA 
TGTAATAAGC CACAAGAAGC TTTTGGTTTT GAACAAGCAG CAAGGGACTA TACACTTCGT 
ACATTTGGAG AAATGGCAGA TGCATTCAAA TCTGATTACT TCAACATGCC AGTCCATATG 
GTTCCCACAG AGCTGGTGGA GAAGGAGTTT TGGCGGCTCG TAAGCACTAT TGAAGAAGAC 
GTCACGGTGG AGTATGGAGC TGATATTGCT TCAAAAGAGT TTGGCAGTGG CTTTCCTGTT 
CGAGATGGGA AAATCAAGAT TTCTCCTGAG GAAGAGGAAT ACCTGGATAG TGGCTGGAAT 
TTGAACAACA TGCCAGTGAT GGAGCAGTCT GTCCTTGCTC ATATTACTGC TGATATATGT 
GGCATGAAAC TCCCCTGGTT GTATGTGGGC ATGTGCTTTT CTTCATTCTG TTGGCACATT 
GAAGACCACT GGAGCTATTC AATCAACTAC CTACACTGGG GTGAGCCAAA AACCTGGTAT 
GGAGTTCCAG GCTACGCTGC TGAGCAGCTA GAAAACGTGA TGAAGAAGCT CGCTCCTGAG 
CTCTTTGTGT CCCAGCCGGA CCTGCTCCAC CAGCTGGTGA CCATCATGAA CCCCAACACT 
CTGATGACTC ATGAAGTGCC TGTTTATCGA ACGAATCAGT GTGCTGGGGA GTTTGTAATT 
ACGTTTCCAA GAGCCTACCA CAGTGGCTTC AACCAAGGTT TTAATTTTGC CGAGGCTGTT 
AACTTCTGCA CTGTTGATTG GCTGCCATTG GGCCGCCAGT GTGTGGAGCA TTATCGCTTG 
CTGCACCGTT ATTGTGTCTT TTCCCACGAT GAAATGATAT GCAAAATGGC TTCTAAGGCT 
GATGTATTAG ACGTTGTGGT AGCGTCAACT GTTCAGAAGG ACATGGCCAT CATGATCGAG 
GACGAGAAAG CTTTAAGGGA AACTGTTCGG AAATTGGGAG TAATTGATTC TGAAAGAATG 
GATTTTGAGC TGTTGCCAGA TGATGAGCGG CAGTGTATAA AATGCAAAAC TACATGCTTC 
ATGTCTGCCA TCTCCTGCTC TTGTAAACCT GGCCTACTTG TTTGCCTACA TCATGTGAAA 
GAATTGTGTT CTTGCCCCCC GTATAAGTAT AACCTGCGGT ATAGATACAC TCTGGATGAT 
CTCTATCCAA TGATGAATGC ATTAAAGCTT CGAGCAGAGT CTTACAATGA ATGGGCCTTA 
AATGTGAATG AAGCCTTGGA AGCAAAGATC AATAAGAAGA AAAGTCTTGT CAGCTTTAAA 
GCTTTGATTG AAGAATCAGA AATGAAAAAA TTCCCAGACA ATGATCTTTT GCGTCACCTT 
CGACTAGTCA CGCAGGATGC AGAAAAGTGT GCCTCTGTTG CCCAGCAGTT GCTTAATGGC 
AAAAGGCAAA CAAGGTATCG ATCTGGTGGA GGAAAGTCTC AGAATCAGCT GACGGTGAAT 
GAACTTAGAC AGTTTGTGAC TCAGCTGTAT GCTCTGCCGT GCGTCCTTAG TCAGACACCG 
TTGCTAAAGG ATCTCTTGAA TCGTGTGGAA GATTTCCAAC AGCAAAGCCA AAAACTACTG 
TCTGAGGAAA TGCCTAGCGC TGCCGAGCTA CAGGAGTTGC TGGATGTCAG CTTTGAATTT 
GATGTTGAGC TTCCACAGCT CACTGAGATG CGCATTCGCT TAGAGCAGGC CCGCTGGCTG 
GAAGAGGTTC AGCAGGCTTG CCTGGACTCC AGCTCCCTTT CTTTGGATGA TATGAGACGA 
CTCATAGACT TAGGGGTGGG GCTGGCTCCA TATTCTGCTG TGGAGAAAGC TATGGCCCGC 
CTGCAGGAGC TGCTAACAGT GTCAGAACAC TGGGACGACA AAGCCAAGAG TCTCCTCAGA 
GCAAGACCAC GGCATTCTTT GAGTAGCCTT GCTACAGCAG TCAAAGAGAT GGAGGAGATC 
CCCGCCTACC TGCCCAATGG TACAGTCCTG AAGGATTCTG TGCAAAGAGC CAGAGACTGG 
GTTCAGGATG TGGATGCATT GCAGGCAGGA GGACGTGTGC CAGTGTTAGA GACACTCATT 
GAACTTGTTG CAAGAGGCCG ATCTATCCCA GTACATCTCA ATTCTTTACC AAGATTGGAA 
ATGTTAGTAG CTGAAGTTCA TGCATGGAAA GAATGTGCTG CTAAAACATT CTTGCCTGAG 
AATTCTACAT ATTCTCTGTT AGAGGTTCTT TGCCCTCGGT GTGACATTGG CCTTTTGGGG 
CTGAAAAGGA AACAGAGAAA GTTGAAGGAG CCCTTGCCGA GTGGAAAGAA GAGAAGCACC 
AAGTTAGAGA GCCTGAGCGA TCTGGAGAGG GCTCTGATGG AGAGCAAAGA GACTGCGGCA 
GCTATGGCAA CTCTTGGGGA AGCTCGCCTA CGAGAGATGG AAGCTTTGCA GTCTCTCAGA 
TTTGCCAATG AAGAGAAATT GCTGTCACCT GTCCAAGACT TAGAGATGAA AGTCTGCTTG 
TGTCAGAAGA CTCCAGCCAC TCCCATGATT CAGTGTGAAC TCTGCAGGGA TGCTTTCCAT 
ACTAGTTGTG TGGCAGCACC CAGTATTTCA CAAAGCTCCC GAATCTGGCT TTGTCCTCAC 
TGTCGGAGGT CAGAGAAGCC TCCATTAGAG AAAATTCTGC CTCTGCTGGC CTCCCTACAG 
CGTATACGAG TTCGTCTTCC TGAAGGAGAT GCACTCCGAT ACATGATTGA AAGAACCGTG 
AACTGGCAAC ATAGAGCCCA GCAGCTGCTT TCTTCAGGGA ATCTAAAACT TGTGCAAGAC 
CAAGTGGGTT CTGGACTGTT GTCTAGCAGA TGGCCAGCCT CAGCAGGACA GGCATCAGCA 
ACAGACAAGG TGTCTCAGCC TCCTGGCACA ACATCCTTTT CATTGCCCGA TGACTGGGAC 
AACAGAACCT CATATTTACA CTCTCCCTTC TCTACTGGAC AGAGTTGTCT CCCCCTCCAT 
GGTTTGAGTC CAGAAGTGAA TGAATTGCTG ATGGAAGCCC AGCTGCTCCA GGTATCCCTT 
CCTGAAATTC AGGAGCTTTA TCAGACTCTA CTTACTAAGC CAAGCTCTGT TCAGCAAGCT 
GACCGAAGTT CACCAGTTAG ATCCAGCAGT GAGAAGAACG ATTGCCTTCG AGGGAAACGA 
GATGCAATCA ACAGTCCTGA GAGGAAACTG AAAAGGCGCC CAGAAAGAGA AGGCCTTCCT 
AGCGAGCGGT GGGATCGAGT TAAACACATG CGGACCCCCC AAAAGAAGAA AATCAAACTG 
AGTCACCCCA AGGACATGGA CAGTTTCAAG TTAGAAAGAG AGCGTAGCTA TGACTTAGTG 
CGTAATGCTG AAACTCATTC CCTGCCCTCA GACACATCCT ACTCCGAACA GGAGGACTCT 
GAGGATGAAG ATGCCATCTG TCCAGCTGTG AGCTGCCTGC AGCCAGAAGG AGATGAGGTG 
GACTGGGTCC AGTGTGATGG CAGCTGCAAT CAGTGGTTTC ACCAGGTCTG TGTTGGTGTC 
TCTCCAGAGA TGGCAGAGAA GGAAGACTAC ATCTGTGTGC GCTGTACTGG GAAGGACGCA 
CCAAGCCGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690855.2
CDS	162..4796
Translation	MEPATTLPPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKPHDIPQRQSVQPAETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCTTPKWENEKEMKSTIKQEPTEKKDCELESEKEKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLVPPLHDVPKGDWRCPKCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQQSQKLLSEEMPSAAELQELLDVSFEFDVELPQLTEMRIRLEQARWLEEVQQACLDSSSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLRARPRHSLSSLATAVKEMEEIPAYLPNGTVLKDSVQRARDWVQDVDALQAGGRVPVLETLIELVARGRSIPVHLNSLPRLEMLVAEVHAWKECAAKTFLPENSTYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPSGKKRSTKLESLSDLERALMESKETAAAMATLGEARLREMEALQSLRFANEEKLLSPVQDLEMKVCLCQKTPATPMIQCELCRDAFHTSCVAAPSISQSSRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKLVQDQVGSGLLSSRWPASAGQASATDKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGQSCLPLHGLSPEVNELLMEAQLLQVSLPEIQELYQTLLTKPSSVQQADRSSPVRSSSEKNDCLRGKRDAINSPERKLKRRPEREGLPSERWDRVKHMRTPQKKKIKLSHPKDMDSFKLERERSYDLVRNAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTGKDAPSRK

Target ORF information:

RefSeq Version	NM_152895.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 5B (Kdm5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152895.2

ORF Insert Sequence:

ATGGAGCCGG CCACCACGCT GCCCCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GAGAGTTCCT GCCTCCCCCC GAGTGTCCGG TCTTCGAGCC GAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGG 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TCGCTTGTGA TGTCGATAAA 
CTTCATTTCA CCCCGCGGAT CCAGAGGCTG AATGAGCTGG AGGCCCAAAC ACGTGTAAAA 
TTGAATTTCC TGGACCAGAT TGCAAAGTAT TGGGAGCTAC AAGGAAGTAC TCTGAAGATT 
CCCCACGTGG AGAGAAAGAT CTTAGACTTG TTCCAGCTAA ATAAGTTAGT CGCAGAAGAA 
GGTGGGTTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCCAC CAAGATGGGT 
TTTGCTCCTG GTAAAGCTGT GGGCTCACAT ATCAGGGGAC ATTATGAGCG AATACTCAAT 
CCATACAATT TGTTCCTGTC TGGAGACAGT CTGAGGTGTC TTCAGAAGCC TAACCTGACC 
TCCGACACAA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCCGCAGAAA CCTGTCCTCC GGCCCGCCGA GCAAAGCGCA TGAGAGCAGA GGCTATGAAT 
ATTAAAATTG AGCCAGAAGA GGCAACAGAA GCCAGGACTC ATAATCTGAG ACGCCGAATG 
GGTTGCACAA CTCCAAAATG GGAAAATGAG AAAGAAATGA AAAGCACCAT CAAGCAAGAG 
CCCACTGAGA AGAAAGACTG CGAGTTAGAA AGTGAGAAGG AGAAGCCCAA GAGCCGGGCC 
AAGAAAACGG CCACTGCGGT GGACCTGTAT GTGTGCCTCT TATGTGGAAG TGGCAATGAC 
GAGGACCGAC TCCTACTGTG TGACGGCTGT GATGACAGTT ACCACACCTT CTGCCTGGTT 
CCCCCTCTCC ATGACGTTCC CAAGGGAGAC TGGAGGTGTC CTAAGTGTTT GGCTCAAGAA 
TGTAATAAGC CACAAGAAGC TTTTGGTTTT GAACAAGCAG CAAGGGACTA TACACTTCGT 
ACATTTGGAG AAATGGCAGA TGCATTCAAA TCTGATTACT TCAACATGCC AGTCCATATG 
GTTCCCACAG AGCTGGTGGA GAAGGAGTTT TGGCGGCTCG TAAGCACTAT TGAAGAAGAC 
GTCACGGTGG AGTATGGAGC TGATATTGCT TCAAAAGAGT TTGGCAGTGG CTTTCCTGTT 
CGAGATGGGA AAATCAAGAT TTCTCCTGAG GAAGAGGAAT ACCTGGATAG TGGCTGGAAT 
TTGAACAACA TGCCAGTGAT GGAGCAGTCT GTCCTTGCTC ATATTACTGC TGATATATGT 
GGCATGAAAC TCCCCTGGTT GTATGTGGGC ATGTGCTTTT CTTCATTCTG TTGGCACATT 
GAAGACCACT GGAGCTATTC AATCAACTAC CTACACTGGG GTGAGCCAAA AACCTGGTAT 
GGAGTTCCAG GCTACGCTGC TGAGCAGCTA GAAAACGTGA TGAAGAAGCT CGCTCCTGAG 
CTCTTTGTGT CCCAGCCGGA CCTGCTCCAC CAGCTGGTGA CCATCATGAA CCCCAACACT 
CTGATGACTC ATGAAGTGCC TGTTTATCGA ACGAATCAGT GTGCTGGGGA GTTTGTAATT 
ACGTTTCCAA GAGCCTACCA CAGTGGCTTC AACCAAGGTT TTAATTTTGC CGAGGCTGTT 
AACTTCTGCA CTGTTGATTG GCTGCCATTG GGCCGCCAGT GTGTGGAGCA TTATCGCTTG 
CTGCACCGTT ATTGTGTCTT TTCCCACGAT GAAATGATAT GCAAAATGGC TTCTAAGGCT 
GATGTATTAG ACGTTGTGGT AGCGTCAACT GTTCAGAAGG ACATGGCCAT CATGATCGAG 
GACGAGAAAG CTTTAAGGGA AACTGTTCGG AAATTGGGAG TAATTGATTC TGAAAGAATG 
GATTTTGAGC TGTTGCCAGA TGATGAGCGG CAGTGTATAA AATGCAAAAC TACATGCTTC 
ATGTCTGCCA TCTCCTGCTC TTGTAAACCT GGCCTACTTG TTTGCCTACA TCATGTGAAA 
GAATTGTGTT CTTGCCCCCC GTATAAGTAT AACCTGCGGT ATAGATACAC TCTGGATGAT 
CTCTATCCAA TGATGAATGC ATTAAAGCTT CGAGCAGAGT CTTACAATGA ATGGGCCTTA 
AATGTGAATG AAGCCTTGGA AGCAAAGATC AATAAGAAGA AAAGTCTTGT CAGCTTTAAA 
GCTTTGATTG AAGAATCAGA AATGAAAAAA TTCCCAGACA ATGATCTTTT GCGTCACCTT 
CGACTAGTCA CGCAGGATGC AGAAAAGTGT GCCTCTGTTG CCCAGCAGTT GCTTAATGGC 
AAAAGGCAAA CAAGGTATCG ATCTGGTGGA GGAAAGTCTC AGAATCAGCT GACGGTGAAT 
GAACTTAGAC AGTTTGTGAC TCAGCTGTAT GCTCTGCCGT GCGTCCTTAG TCAGACACCG 
TTGCTAAAGG ATCTCTTGAA TCGTGTGGAA GATTTCCAAC AGCAAAGCCA AAAACTACTG 
TCTGAGGAAA TGCCTAGCGC TGCCGAGCTA CAGGAGTTGC TGGATGTCAG CTTTGAATTT 
GATGTTGAGC TTCCACAGCT CACTGAGATG CGCATTCGCT TAGAGCAGGC CCGCTGGCTG 
GAAGAGGTTC AGCAGGCTTG CCTGGACTCC AGCTCCCTTT CTTTGGATGA TATGAGACGA 
CTCATAGACT TAGGGGTGGG GCTGGCTCCA TATTCTGCTG TGGAGAAAGC TATGGCCCGC 
CTGCAGGAGC TGCTAACAGT GTCAGAACAC TGGGACGACA AAGCCAAGAG TCTCCTCAGA 
GCAAGACCAC GGCATTCTTT GAGTAGCCTT GCTACAGCAG TCAAAGAGAT GGAGGAGATC 
CCCGCCTACC TGCCCAATGG TACAGTCCTG AAGGATTCTG TGCAAAGAGC CAGAGACTGG 
GTTCAGGATG TGGATGCATT GCAGGCAGGA GGACGTGTGC CAGTGTTAGA GACACTCATT 
GAACTTGTTG CAAGAGGCCG ATCTATCCCA GTACATCTCA ATTCTTTACC AAGATTGGAA 
ATGTTAGTAG CTGAAGTTCA TGCATGGAAA GAATGTGCTG CTAAAACATT CTTGCCTGAG 
AATTCTACAT ATTCTCTGTT AGAGGTTCTT TGCCCTCGGT GTGACATTGG CCTTTTGGGG 
CTGAAAAGGA AACAGAGAAA GTTGAAGGAG CCCTTGCCGA GTGGAAAGAA GAGAAGCACC 
AAGTTAGAGA GCCTGAGCGA TCTGGAGAGG GCTCTGATGG AGAGCAAAGA GACTGCGGCA 
GCTATGGCAA CTCTTGGGGA AGCTCGCCTA CGAGAGATGG AAGCTTTGCA GTCTCTCAGA 
TTTGCCAATG AAGAGAAATT GCTGTCACCT GTCCAAGACT TAGAGATGAA AGTCTGCTTG 
TGTCAGAAGA CTCCAGCCAC TCCCATGATT CAGTGTGAAC TCTGCAGGGA TGCTTTCCAT 
ACTAGTTGTG TGGCAGCACC CAGTATTTCA CAAAGCTCCC GAATCTGGCT TTGTCCTCAC 
TGTCGGAGGT CAGAGAAGCC TCCATTAGAG AAAATTCTGC CTCTGCTGGC CTCCCTACAG 
CGTATACGAG TTCGTCTTCC TGAAGGAGAT GCACTCCGAT ACATGATTGA AAGAACCGTG 
AACTGGCAAC ATAGAGCCCA GCAGCTGCTT TCTTCAGGGA ATCTAAAACT TGTGCAAGAC 
CAAGTGGGTT CTGGACTGTT GTCTAGCAGA TGGCCAGCCT CAGCAGGACA GGCATCAGCA 
ACAGACAAGG TGTCTCAGCC TCCTGGCACA ACATCCTTTT CATTGCCCGA TGACTGGGAC 
AACAGAACCT CATATTTACA CTCTCCCTTC TCTACTGGAC AGAGTTGTCT CCCCCTCCAT 
GGTTTGAGTC CAGAAGTGAA TGAATTGCTG ATGGAAGCCC AGCTGCTCCA GGTATCCCTT 
CCTGAAATTC AGGAGCTTTA TCAGACTCTA CTTACTAAGC CAAGCTCTGT TCAGCAAGCT 
GACCGAAGTT CACCAGTTAG ATCCAGCAGT GAGAAGAACG ATTGCCTTCG AGGGAAACGA 
GATGCAATCA ACAGTCCTGA GAGGAAACTG AAAAGGCGCC CAGAAAGAGA AGGCCTTCCT 
AGCGAGCGGT GGGATCGAGT TAAACACATG CGGACCCCCC AAAAGAAGAA AATCAAACTG 
AGTCACCCCA AGGACATGGA CAGTTTCAAG TTAGAAAGAG AGCGTAGCTA TGACTTAGTG 
CGTAATGCTG AAACTCATTC CCTGCCCTCA GACACATCCT ACTCCGAACA GGAGGACTCT 
GAGGATGAAG ATGCCATCTG TCCAGCTGTG AGCTGCCTGC AGCCAGAAGG AGATGAGGTG 
GACTGGGTCC AGTGTGATGG CAGCTGCAAT CAGTGGTTTC ACCAGGTCTG TGTTGGTGTC 
TCTCCAGAGA TGGCAGAGAA GGAAGACTAC ATCTGTGTGC GCTGTACTGG GAAGGACGCA 
CCAAGCCGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone demethylase jumonji AT-rich interactive domain 1B (JARID1B) controls mammary gland development by regulating key developmental and lineage specification genes.
The Journal of biological chemistry289(25)17620-33(2014 Jun)
Zou MR,Cao J,Liu Z,Huh SJ,Polyak K,Yan Q

The histone demethylase Jarid1b ensures faithful mouse development by protecting developmental genes from aberrant H3K4me3.
PLoS genetics9(4)e1003461(2013 Apr)
Albert M,Schmitz SU,Kooistra SM,Malatesta M,Morales Torres C,Rekling JC,Johansen JV,Abarrategui I,Helin K

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Kdm5b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

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