Secisbp2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Secisbp2
Entrez Gene ID	75420
Full Name	SECIS binding protein 2
Synonyms	2210413N07Rik,2810012K13Rik,SBP2
General protein information	Preferred Names SECIS binding protein 2 Names selenocysteine insertion sequence-binding protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A5
Summary	The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in a similar gene in human have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006516964.3	XP_006517027.1	selenocysteine insertion sequence-binding protein 2 isoform X1
XM_006516966.1	XP_006517029.1	selenocysteine insertion sequence-binding protein 2 isoform X3
XM_006516966.2	XP_006517029.1	selenocysteine insertion sequence-binding protein 2 isoform X1
NM_001308448.1	NP_001295377.1	selenocysteine insertion sequence-binding protein 2 isoform 2
XM_006516965.3	XP_006517028.1	selenocysteine insertion sequence-binding protein 2 isoform X2
NM_029279.2	NP_083555.1	selenocysteine insertion sequence-binding protein 2 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SECISBP2	XP_520120.3
Rattus norvegicus (Norway rat)	Secisbp2	NP_076492.1
Homo sapiens (human)	SECISBP2	NP_076982.3
Canis lupus familiaris (dog)	SECISBP2	XP_005616013.1
Mus musculus (house mouse)	Secisbp2	NP_083555.1
Gallus gallus (chicken)	SECISBP2	XP_424425.4
Bos taurus (cattle)	SECISBP2	XP_002689905.2
Xenopus tropicalis (tropical clawed frog)	secisbp2	NP_001090731.1
	SECISBP2	XP_001090657.1
Danio rerio (zebrafish)	LOC101883890	XP_005165670.1

Gene Ontology
Bibliography

Related articles in PubMed

Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.
Fu J, Fujisawa H, Follman B, Liao XH, Dumitrescu AM
Endocrinology158(12)4317-4330(2017 Dec)

The RNA-binding protein Secisbp2 differentially modulates UGA codon reassignment and RNA decay.
Fradejas-Villar N, Seeher S, Anderson CB, Doengi M, Carlson BA, Hatfield DL, Schweizer U, Howard MT
Nucleic acids research45(7)4094-4107(2017 Apr)

Secisbp2 is essential for embryonic development and enhances selenoprotein expression.
Seeher S, Atassi T, Mahdi Y, Carlson BA, Braun D, Wirth EK, Klein MO, Reix N, Miniard AC, Schomburg L, Hatfield DL, Driscoll DM, Schweizer U
Antioxidants & redox signaling21(6)835-49(2014 Aug)

Impaired selenoprotein expression in brain triggers striatal neuronal loss leading to co-ordination defects in mice.
Seeher S, Carlson BA, Miniard AC, Wirth EK, Mahdi Y, Hatfield DL, Driscoll DM, Schweizer U
The Biochemical journal462(1)67-75(2014 Aug)

A novel insight into the mechanism of mammalian selenoprotein synthesis.
Kossinova O, Malygin A, Krol A, Karpova G
RNA (New York, N.Y.)19(8)1147-58(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Selenocysteine insertion sequence binding protein 2 (SBP2)conditional knockout (iCKO) mice replicate the thyroid phenotype of SBP2 deficiency for investigating selenoprotein biology relevant to human disease.
Title: Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.

The results demonstrate that Secisbp2 is not strictly required for Sec incorporation and has a distinct role in stabilizing mRNAs that can be separated from its effects on UGA-redefinition
Title: The RNA-binding protein Secisbp2 differentially modulates UGA codon reassignment and RNA decay.

Secisbp2 is essential for embryonic development and enhances selenoprotein expression.
Title: Secisbp2 is essential for embryonic development and enhances selenoprotein expression.

SBP2/SECIS complex activates eEFSec by directing functional interactions between Domain IV and the ribosome to promote Sec-tRNA(Sec) binding and accommodation into the ribosomal A-site
Title: The selenocysteine-specific elongation factor contains a novel and multi-functional domain.

Selenocysteine insertion sequence (SECIS)-binding protein 2 alters conformational dynamics of residues involved in tRNA accommodation in 80 S ribosomes.
Title: Selenocysteine insertion sequence (SECIS)-binding protein 2 alters conformational dynamics of residues involved in tRNA accommodation in 80 S ribosomes.

The following Secisbp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Secisbp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06713	NM_029279.2 Latest version!	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu71309	NM_001308448.1 Latest version!	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu48571	XM_006516964.3 Latest version!	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu48572	XM_006516965.3 Latest version!	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu48573	XM_006516966.2 Latest version!	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu48573	XM_006516966.1	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Secisbp2 ( NM_029279.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083555.1 Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06713
Clone ID Related Accession (Same CDS sequence)	NM_029279.2
Accession Version	NM_029279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Mus musculus(house mouse)
Product	selenocysteine insertion sequence-binding protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC126547.4, AK168634.1, BP760396.1 and CA329104.1. On May 5, 2015 this sequence version replaced NM_029279.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK156076.1, AK168634.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG AGCGGCCGCG GGAGCCGGAC GGCGAGGATA GCATCAAGTT GTCAGCTGAT 
GTCAAACCAT TCGTCCCTAA GTTTGCTGGG CTCAACGTGG CGTGGTCAGA GTCCTCAGAG 
ACACGTGTCT TCCCAGGCTG TGCGGCCACC TACTATCCAT TTGTACAGGA ACCACCAGCA 
GCTGAACAGA AAATGTATCC CGAAGACATG GCTTTCGGAG CCCCCACCTT TCCAGCACAG 
TACGTGTCTT CTGAGATAGC GCTGCATCCT TTTGCCTATC CCACTTACAC CCTAGAGTCC 
GCACAGAGTG TTTGCTCAGT GCCAACCCTG CAGTACGACT ACAGCCAAGC ACGGTGTCAC 
CCAGGCTTTC GGACAGCAAA GCCCCGGCAT GAGCACGTGT GCCCTCCACC TCAGGAAGCA 
AAAGGTGTAT TTAAGAAAAA ACCCTCTGAT GAGAGAAGAG CATGTGAAGA GCAAAAGTCA 
AGCAGCAGAA GGGCTGACAA TGCGGTGCCC TGTGAGGCGA GACCAGCCAG GGGGTCCAGT 
CACCTGTCCT CTCGAACTGA GAGCAGTTTG AAATCTGATG GTTACCACAA GCGACCCGAC 
CGCAAGTCCA GAATCCTTGC GAAGAGTGCA TCTACCTCTA AACCTGAATT TGAGTTTAGT 
AGGTTAGACT TTCCTGAACT GCAGAGTCCA AAGAACAGTA ACATGCCAGA GACACAGAAG 
CCGCCCAGGT GGGGGCCTCT TGGCCCTGCT GCCAGTAACA TGCCTCTCCT AGGAGACGTC 
GGCAAGCCCG TCGCAGATAT GGTAGAGGGC AAAATGGTGA AGAGCGATCA CACTGATGGA 
GCTGTGACCA GTAATGCCAC TACCAGTTCC CCTTCATGTA CCCAAGAGTT GTCTTGGACA 
CCAATGGGTT ATATTGTTCG GCAGACAGTG TCTTCAGATT CAGCAGCAGC CACTGAAAAT 
GTGACTTCCA TGATAAACCT AAAGAAGACT ACTTCATCAG CTGATGCTAA AAATGTTAGT 
GTGACATCTG AGGCTTTATC TTCAAATCCT TCCTACAACA GAGAAAAGCG TGTTTATCCT 
GCTCCAAAGG CCAAAGCATC ACAAGGAGGT GAACTTGAAC AAAACGAAAG CTCCAAAAAG 
AATAAGAAAA AGAAAGAGAA GTCTAAACCG AGTTATGAAG TCCTGACGGT TCAGGAGCCG 
CCAAGGATTG AAGATGCAGA GGAATTCCCC AACCTGTCAG TTGCGTCGGA AAGAAGACAC 
AGAGGGCAAT CACCGAAGCT TCACAGTAAA CAGCAGACGC AGAATGAATT TAAAACAAGT 
GGGAAGAAGA GCCAGGTCCC AGTGCAGCTG GACCTGGGGG GCATGCTGGC CGCGCTGGAG 
AAGCAGCAGC AGCAGCAGCA CGCCTCGCAC GCCAAGCCAT CCTCCAGACC CGTCGTGTTC 
TCAGTTGGAG CAGTGCCAGT CCTGTCCAAG GATGCCTCCT CCAGTGAGAG GGGACGCCGC 
TCCAGTCAGA TGAAGACCCC ACACAACCCC CTGGACTCCA GTGCCCCCCT GATGAAGAAG 
GGGAAGCAGA GGGAGATACC TAAGGCCAAG AAGCCCACCT CACTGAAGAA GATAATTTTG 
AAAGAACGGC AAGAGAGGAT GCAGCAGCGA CTCCAAGAAA GTGCTGTGAG CCTGACGGTG 
GCCAGTGATG ACTCACAGGA TGTGGAGAGT GGCGCCAGTA ACCAAACCCC CAGTCAGGAC 
AACCCCACAG GTCCAGAGAA GACAGAAGAA TCAGTGTCTT CTACACCTGT GGTTGAGGGT 
GAGTCAGAGG AGCCAGCTGG CACAGAGTTC CAGAGGGACC CAGAGGCTTG CCAGCCTGCC 
CCTGACAGTG CCACCTTCCC CAAGATCCAC AGCCGGAGGT TCCGGGACTA CTGCAGCCAG 
ATGCTTAGTA AAGAAGTAGA TGCTTGTGTC ACGGGTCTGC TCAAGGAGCT GGTGCGCTTC 
CAAGACCGCA TGTACCAGAA GGATCCCGTC AAGGCCAAGA CAAAACGGCG GCTCGTGCTG 
GGGCTGAGGG AGGTCCTGAA ACACCTGAAG CTCAGGAAGC TGAAGTGCAT CATCATCTCT 
CCCAACTGTG AGAAGACCCA GTCTAAAGGT GGGCTGGACG ACACGCTGCA CACCATCATC 
GACTGCGCCT GCGAGCAGAA CATCCCCTTC GTGTTTGCAC TCAATCGCAA GGCTCTGGGG 
CGGAGCCTGA ATAAAGCTGT TCCTGTCAGC ATTGTAGGGA TCTTCAGCTA CGATGGGGCC 
CAGGACCAGT TCCACAAGAT GGTTGAGCTG ACCATGGCAG CCCGTCAGGC ATACAAGACC 
ATGTTGGAGA CAATGCGGCA GGAACAGGCA GGAGAACCTG GACCTCAGTC CCCTCCCAGC 
CCACCCATGC AAGACCCCAT CCCATCCACG GAAGAAGGCA CCCTCCCTTC CACTGGAGAA 
GAGCCACACT ACATTGAGAT TTGGAAAAAG CACCTGGAAG CGTACAGTCA GCGTGCCCTG 
GAGCTGGAAG ACTCACTGGA GGCGTCAACC TCTCAGATGA TGAACTTGAA TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083555.1
CDS	78..2654
Translation	MASERPREPDGEDSIKLSADVKPFVPKFAGLNVAWSESSETRVFPGCAATYYPFVQEPPAAEQKMYPEDMAFGAPTFPAQYVSSEIALHPFAYPTYTLESAQSVCSVPTLQYDYSQARCHPGFRTAKPRHEHVCPPPQEAKGVFKKKPSDERRACEEQKSSSRRADNAVPCEARPARGSSHLSSRTESSLKSDGYHKRPDRKSRILAKSASTSKPEFEFSRLDFPELQSPKNSNMPETQKPPRWGPLGPAASNMPLLGDVGKPVADMVEGKMVKSDHTDGAVTSNATTSSPSCTQELSWTPMGYIVRQTVSSDSAAATENVTSMINLKKTTSSADAKNVSVTSEALSSNPSYNREKRVYPAPKAKASQGGELEQNESSKKNKKKKEKSKPSYEVLTVQEPPRIEDAEEFPNLSVASERRHRGQSPKLHSKQQTQNEFKTSGKKSQVPVQLDLGGMLAALEKQQQQQHASHAKPSSRPVVFSVGAVPVLSKDASSSERGRRSSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERMQQRLQESAVSLTVASDDSQDVESGASNQTPSQDNPTGPEKTEESVSSTPVVEGESEEPAGTEFQRDPEACQPAPDSATFPKIHSRRFRDYCSQMLSKEVDACVTGLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKTQSKGGLDDTLHTIIDCACEQNIPFVFALNRKALGRSLNKAVPVSIVGIFSYDGAQDQFHKMVELTMAARQAYKTMLETMRQEQAGEPGPQSPPSPPMQDPIPSTEEGTLPSTGEEPHYIEIWKKHLEAYSQRALELEDSLEASTSQMMNLNL

Target ORF information:

RefSeq Version	NM_029279.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029279.2

ORF Insert Sequence:

ATGGCGTCGG AGCGGCCGCG GGAGCCGGAC GGCGAGGATA GCATCAAGTT GTCAGCTGAT 
GTCAAACCAT TCGTCCCTAA GTTTGCTGGG CTCAACGTGG CGTGGTCAGA GTCCTCAGAG 
ACACGTGTCT TCCCAGGCTG TGCGGCCACC TACTATCCAT TTGTACAGGA ACCACCAGCA 
GCTGAACAGA AAATGTATCC CGAAGACATG GCTTTCGGAG CCCCCACCTT TCCAGCACAG 
TACGTGTCTT CTGAGATAGC GCTGCATCCT TTTGCCTATC CCACTTACAC CCTAGAGTCC 
GCACAGAGTG TTTGCTCAGT GCCAACCCTG CAGTACGACT ACAGCCAAGC ACGGTGTCAC 
CCAGGCTTTC GGACAGCAAA GCCCCGGCAT GAGCACGTGT GCCCTCCACC TCAGGAAGCA 
AAAGGTGTAT TTAAGAAAAA ACCCTCTGAT GAGAGAAGAG CATGTGAAGA GCAAAAGTCA 
AGCAGCAGAA GGGCTGACAA TGCGGTGCCC TGTGAGGCGA GACCAGCCAG GGGGTCCAGT 
CACCTGTCCT CTCGAACTGA GAGCAGTTTG AAATCTGATG GTTACCACAA GCGACCCGAC 
CGCAAGTCCA GAATCCTTGC GAAGAGTGCA TCTACCTCTA AACCTGAATT TGAGTTTAGT 
AGGTTAGACT TTCCTGAACT GCAGAGTCCA AAGAACAGTA ACATGCCAGA GACACAGAAG 
CCGCCCAGGT GGGGGCCTCT TGGCCCTGCT GCCAGTAACA TGCCTCTCCT AGGAGACGTC 
GGCAAGCCCG TCGCAGATAT GGTAGAGGGC AAAATGGTGA AGAGCGATCA CACTGATGGA 
GCTGTGACCA GTAATGCCAC TACCAGTTCC CCTTCATGTA CCCAAGAGTT GTCTTGGACA 
CCAATGGGTT ATATTGTTCG GCAGACAGTG TCTTCAGATT CAGCAGCAGC CACTGAAAAT 
GTGACTTCCA TGATAAACCT AAAGAAGACT ACTTCATCAG CTGATGCTAA AAATGTTAGT 
GTGACATCTG AGGCTTTATC TTCAAATCCT TCCTACAACA GAGAAAAGCG TGTTTATCCT 
GCTCCAAAGG CCAAAGCATC ACAAGGAGGT GAACTTGAAC AAAACGAAAG CTCCAAAAAG 
AATAAGAAAA AGAAAGAGAA GTCTAAACCG AGTTATGAAG TCCTGACGGT TCAGGAGCCG 
CCAAGGATTG AAGATGCAGA GGAATTCCCC AACCTGTCAG TTGCGTCGGA AAGAAGACAC 
AGAGGGCAAT CACCGAAGCT TCACAGTAAA CAGCAGACGC AGAATGAATT TAAAACAAGT 
GGGAAGAAGA GCCAGGTCCC AGTGCAGCTG GACCTGGGGG GCATGCTGGC CGCGCTGGAG 
AAGCAGCAGC AGCAGCAGCA CGCCTCGCAC GCCAAGCCAT CCTCCAGACC CGTCGTGTTC 
TCAGTTGGAG CAGTGCCAGT CCTGTCCAAG GATGCCTCCT CCAGTGAGAG GGGACGCCGC 
TCCAGTCAGA TGAAGACCCC ACACAACCCC CTGGACTCCA GTGCCCCCCT GATGAAGAAG 
GGGAAGCAGA GGGAGATACC TAAGGCCAAG AAGCCCACCT CACTGAAGAA GATAATTTTG 
AAAGAACGGC AAGAGAGGAT GCAGCAGCGA CTCCAAGAAA GTGCTGTGAG CCTGACGGTG 
GCCAGTGATG ACTCACAGGA TGTGGAGAGT GGCGCCAGTA ACCAAACCCC CAGTCAGGAC 
AACCCCACAG GTCCAGAGAA GACAGAAGAA TCAGTGTCTT CTACACCTGT GGTTGAGGGT 
GAGTCAGAGG AGCCAGCTGG CACAGAGTTC CAGAGGGACC CAGAGGCTTG CCAGCCTGCC 
CCTGACAGTG CCACCTTCCC CAAGATCCAC AGCCGGAGGT TCCGGGACTA CTGCAGCCAG 
ATGCTTAGTA AAGAAGTAGA TGCTTGTGTC ACGGGTCTGC TCAAGGAGCT GGTGCGCTTC 
CAAGACCGCA TGTACCAGAA GGATCCCGTC AAGGCCAAGA CAAAACGGCG GCTCGTGCTG 
GGGCTGAGGG AGGTCCTGAA ACACCTGAAG CTCAGGAAGC TGAAGTGCAT CATCATCTCT 
CCCAACTGTG AGAAGACCCA GTCTAAAGGT GGGCTGGACG ACACGCTGCA CACCATCATC 
GACTGCGCCT GCGAGCAGAA CATCCCCTTC GTGTTTGCAC TCAATCGCAA GGCTCTGGGG 
CGGAGCCTGA ATAAAGCTGT TCCTGTCAGC ATTGTAGGGA TCTTCAGCTA CGATGGGGCC 
CAGGACCAGT TCCACAAGAT GGTTGAGCTG ACCATGGCAG CCCGTCAGGC ATACAAGACC 
ATGTTGGAGA CAATGCGGCA GGAACAGGCA GGAGAACCTG GACCTCAGTC CCCTCCCAGC 
CCACCCATGC AAGACCCCAT CCCATCCACG GAAGAAGGCA CCCTCCCTTC CACTGGAGAA 
GAGCCACACT ACATTGAGAT TTGGAAAAAG CACCTGGAAG CGTACAGTCA GCGTGCCCTG 
GAGCTGGAAG ACTCACTGGA GGCGTCAACC TCTCAGATGA TGAACTTGAA TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Secisbp2 ( NM_001308448.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001295377.1 Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu71309
Clone ID Related Accession (Same CDS sequence)	NM_001308448.1
Accession Version	NM_001308448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Mus musculus(house mouse)
Product	selenocysteine insertion sequence-binding protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC126547.4, AK012732.1, AK168634.1, BP760396.1 and CA329104.1. On May 5, 2015 this sequence version replaced XM_011244414.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (2) that has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC144795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATCCCG AAGACATGGC TTTCGGAGCC CCCACCTTTC CAGCACAGTA CGTGTCTTCT 
GAGATAGCGC TGCATCCTTT TGCCTATCCC ACTTACACCC TAGAGTCCGC ACAGAGTGTT 
TGCTCAGTGC CAACCCTGCA GTACGACTAC AGCCAAGCAC GGTGTCACCC AGGCTTTCGG 
ACAGCAAAGC CCCGGCATGA GCACGTGTGC CCTCCACCTC AGGAAGCAAA AGGTGTATTT 
AAGAAAAAAC CCTCTGATGA GAGAAGAGCA TGTGAAGAGC AAAAGTCAAG CAGCAGAAGG 
GCTGACAATG CGGTGCCCTG TGAGGCGAGA CCAGCCAGGG GGTCCAGTCA CCTGTCCTCT 
CGAACTGAGA GCAGTTTGAA ATCTGATGGT TACCACAAGC GACCCGACCG CAAGTCCAGA 
ATCCTTGCGA AGAGTGCATC TACCTCTAAA CCTGAATTTG AGTTTAGTAG GTTAGACTTT 
CCTGAACTGC AGAGTCCAAA GAACAGTAAC ATGCCAGAGA CACAGAAGCC GCCCAGGTGG 
GGGCCTCTTG GCCCTGCTGC CAGTAACATG CCTCTCCTAG GAGACGTCGG CAAGCCCGTC 
GCAGATATGG TAGAGGGCAA AATGGTGAAG AGCGATCACA CTGATGGAGC TGTGACCAGT 
AATGCCACTA CCAGTTCCCC TTCATGTACC CAAGAGTTGT CTTGGACACC AATGGGTTAT 
ATTGTTCGGC AGACAGTGTC TTCAGATTCA GCAGCAGCCA CTGAAAATGT GACTTCCATG 
ATAAACCTAA AGAAGACTAC TTCATCAGCT GATGCTAAAA ATGTTAGTGT GACATCTGAG 
GCTTTATCTT CAAATCCTTC CTACAACAGA GAAAAGCGTG TTTATCCTGC TCCAAAGGCC 
AAAGCATCAC AAGGAGGTGA ACTTGAACAA AACGAAAGCT CCAAAAAGAA TAAGAAAAAG 
AAAGAGAAGT CTAAACCGAG TTATGAAGTC CTGACGGTTC AGGAGCCGCC AAGGATTGAA 
GATGCAGAGG AATTCCCCAA CCTGTCAGTT GCGTCGGAAA GAAGACACAG AGGGCAATCA 
CCGAAGCTTC ACAGTAAACA GCAGACGCAG AATGAATTTA AAACAAGTGG GAAGAAGAGC 
CAGGTCCCAG TGCAGCTGGA CCTGGGGGGC ATGCTGGCCG CGCTGGAGAA GCAGCAGCAG 
CAGCAGCACG CCTCGCACGC CAAGCCATCC TCCAGACCCG TCGTGTTCTC AGTTGGAGCA 
GTGCCAGTCC TGTCCAAGGA TGCCTCCTCC AGTGAGAGGG GACGCCGCTC CAGTCAGATG 
AAGACCCCAC ACAACCCCCT GGACTCCAGT GCCCCCCTGA TGAAGAAGGG GAAGCAGAGG 
GAGATACCTA AGGCCAAGAA GCCCACCTCA CTGAAGAAGA TAATTTTGAA AGAACGGCAA 
GAGAGGATGC AGCAGCGACT CCAAGAAAGT GCTGTGAGCC TGACGGTGGC CAGTGATGAC 
TCACAGGATG TGGAGAGTGG CGCCAGTAAC CAAACCCCCA GTCAGGACAA CCCCACAGGT 
CCAGAGAAGA CAGAAGAATC AGTGTCTTCT ACACCTGTGG TTGAGGGTGA GTCAGAGGAG 
CCAGCTGGCA CAGAGTTCCA GAGGGACCCA GAGGCTTGCC AGCCTGCCCC TGACAGTGCC 
ACCTTCCCCA AGATCCACAG CCGGAGGTTC CGGGACTACT GCAGCCAGAT GCTTAGTAAA 
GAAGTAGATG CTTGTGTCAC GGGTCTGCTC AAGGAGCTGG TGCGCTTCCA AGACCGCATG 
TACCAGAAGG ATCCCGTCAA GGCCAAGACA AAACGGCGGC TCGTGCTGGG GCTGAGGGAG 
GTCCTGAAAC ACCTGAAGCT CAGGAAGCTG AAGTGCATCA TCATCTCTCC CAACTGTGAG 
AAGACCCAGT CTAAAGGTGG GCTGGACGAC ACGCTGCACA CCATCATCGA CTGCGCCTGC 
GAGCAGAACA TCCCCTTCGT GTTTGCACTC AATCGCAAGG CTCTGGGGCG GAGCCTGAAT 
AAAGCTGTTC CTGTCAGCAT TGTAGGGATC TTCAGCTACG ATGGGGCCCA GGACCAGTTC 
CACAAGATGG TTGAGCTGAC CATGGCAGCC CGTCAGGCAT ACAAGACCAT GTTGGAGACA 
ATGCGGCAGG AACAGGCAGG AGAACCTGGA CCTCAGTCCC CTCCCAGCCC ACCCATGCAA 
GACCCCATCC CATCCACGGA AGAAGGCACC CTCCCTTCCA CTGGAGAAGA GCCACACTAC 
ATTGAGATTT GGAAAAAGCA CCTGGAAGCG TACAGTCAGC GTGCCCTGGA GCTGGAAGAC 
TCACTGGAGG CGTCAACCTC TCAGATGATG AACTTGAATT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295377.1
CDS	265..2649
Translation	MYPEDMAFGAPTFPAQYVSSEIALHPFAYPTYTLESAQSVCSVPTLQYDYSQARCHPGFRTAKPRHEHVCPPPQEAKGVFKKKPSDERRACEEQKSSSRRADNAVPCEARPARGSSHLSSRTESSLKSDGYHKRPDRKSRILAKSASTSKPEFEFSRLDFPELQSPKNSNMPETQKPPRWGPLGPAASNMPLLGDVGKPVADMVEGKMVKSDHTDGAVTSNATTSSPSCTQELSWTPMGYIVRQTVSSDSAAATENVTSMINLKKTTSSADAKNVSVTSEALSSNPSYNREKRVYPAPKAKASQGGELEQNESSKKNKKKKEKSKPSYEVLTVQEPPRIEDAEEFPNLSVASERRHRGQSPKLHSKQQTQNEFKTSGKKSQVPVQLDLGGMLAALEKQQQQQHASHAKPSSRPVVFSVGAVPVLSKDASSSERGRRSSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERMQQRLQESAVSLTVASDDSQDVESGASNQTPSQDNPTGPEKTEESVSSTPVVEGESEEPAGTEFQRDPEACQPAPDSATFPKIHSRRFRDYCSQMLSKEVDACVTGLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKTQSKGGLDDTLHTIIDCACEQNIPFVFALNRKALGRSLNKAVPVSIVGIFSYDGAQDQFHKMVELTMAARQAYKTMLETMRQEQAGEPGPQSPPSPPMQDPIPSTEEGTLPSTGEEPHYIEIWKKHLEAYSQRALELEDSLEASTSQMMNLNL

Target ORF information:

RefSeq Version	NM_001308448.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308448.1

ORF Insert Sequence:

ATGTATCCCG AAGACATGGC TTTCGGAGCC CCCACCTTTC CAGCACAGTA CGTGTCTTCT 
GAGATAGCGC TGCATCCTTT TGCCTATCCC ACTTACACCC TAGAGTCCGC ACAGAGTGTT 
TGCTCAGTGC CAACCCTGCA GTACGACTAC AGCCAAGCAC GGTGTCACCC AGGCTTTCGG 
ACAGCAAAGC CCCGGCATGA GCACGTGTGC CCTCCACCTC AGGAAGCAAA AGGTGTATTT 
AAGAAAAAAC CCTCTGATGA GAGAAGAGCA TGTGAAGAGC AAAAGTCAAG CAGCAGAAGG 
GCTGACAATG CGGTGCCCTG TGAGGCGAGA CCAGCCAGGG GGTCCAGTCA CCTGTCCTCT 
CGAACTGAGA GCAGTTTGAA ATCTGATGGT TACCACAAGC GACCCGACCG CAAGTCCAGA 
ATCCTTGCGA AGAGTGCATC TACCTCTAAA CCTGAATTTG AGTTTAGTAG GTTAGACTTT 
CCTGAACTGC AGAGTCCAAA GAACAGTAAC ATGCCAGAGA CACAGAAGCC GCCCAGGTGG 
GGGCCTCTTG GCCCTGCTGC CAGTAACATG CCTCTCCTAG GAGACGTCGG CAAGCCCGTC 
GCAGATATGG TAGAGGGCAA AATGGTGAAG AGCGATCACA CTGATGGAGC TGTGACCAGT 
AATGCCACTA CCAGTTCCCC TTCATGTACC CAAGAGTTGT CTTGGACACC AATGGGTTAT 
ATTGTTCGGC AGACAGTGTC TTCAGATTCA GCAGCAGCCA CTGAAAATGT GACTTCCATG 
ATAAACCTAA AGAAGACTAC TTCATCAGCT GATGCTAAAA ATGTTAGTGT GACATCTGAG 
GCTTTATCTT CAAATCCTTC CTACAACAGA GAAAAGCGTG TTTATCCTGC TCCAAAGGCC 
AAAGCATCAC AAGGAGGTGA ACTTGAACAA AACGAAAGCT CCAAAAAGAA TAAGAAAAAG 
AAAGAGAAGT CTAAACCGAG TTATGAAGTC CTGACGGTTC AGGAGCCGCC AAGGATTGAA 
GATGCAGAGG AATTCCCCAA CCTGTCAGTT GCGTCGGAAA GAAGACACAG AGGGCAATCA 
CCGAAGCTTC ACAGTAAACA GCAGACGCAG AATGAATTTA AAACAAGTGG GAAGAAGAGC 
CAGGTCCCAG TGCAGCTGGA CCTGGGGGGC ATGCTGGCCG CGCTGGAGAA GCAGCAGCAG 
CAGCAGCACG CCTCGCACGC CAAGCCATCC TCCAGACCCG TCGTGTTCTC AGTTGGAGCA 
GTGCCAGTCC TGTCCAAGGA TGCCTCCTCC AGTGAGAGGG GACGCCGCTC CAGTCAGATG 
AAGACCCCAC ACAACCCCCT GGACTCCAGT GCCCCCCTGA TGAAGAAGGG GAAGCAGAGG 
GAGATACCTA AGGCCAAGAA GCCCACCTCA CTGAAGAAGA TAATTTTGAA AGAACGGCAA 
GAGAGGATGC AGCAGCGACT CCAAGAAAGT GCTGTGAGCC TGACGGTGGC CAGTGATGAC 
TCACAGGATG TGGAGAGTGG CGCCAGTAAC CAAACCCCCA GTCAGGACAA CCCCACAGGT 
CCAGAGAAGA CAGAAGAATC AGTGTCTTCT ACACCTGTGG TTGAGGGTGA GTCAGAGGAG 
CCAGCTGGCA CAGAGTTCCA GAGGGACCCA GAGGCTTGCC AGCCTGCCCC TGACAGTGCC 
ACCTTCCCCA AGATCCACAG CCGGAGGTTC CGGGACTACT GCAGCCAGAT GCTTAGTAAA 
GAAGTAGATG CTTGTGTCAC GGGTCTGCTC AAGGAGCTGG TGCGCTTCCA AGACCGCATG 
TACCAGAAGG ATCCCGTCAA GGCCAAGACA AAACGGCGGC TCGTGCTGGG GCTGAGGGAG 
GTCCTGAAAC ACCTGAAGCT CAGGAAGCTG AAGTGCATCA TCATCTCTCC CAACTGTGAG 
AAGACCCAGT CTAAAGGTGG GCTGGACGAC ACGCTGCACA CCATCATCGA CTGCGCCTGC 
GAGCAGAACA TCCCCTTCGT GTTTGCACTC AATCGCAAGG CTCTGGGGCG GAGCCTGAAT 
AAAGCTGTTC CTGTCAGCAT TGTAGGGATC TTCAGCTACG ATGGGGCCCA GGACCAGTTC 
CACAAGATGG TTGAGCTGAC CATGGCAGCC CGTCAGGCAT ACAAGACCAT GTTGGAGACA 
ATGCGGCAGG AACAGGCAGG AGAACCTGGA CCTCAGTCCC CTCCCAGCCC ACCCATGCAA 
GACCCCATCC CATCCACGGA AGAAGGCACC CTCCCTTCCA CTGGAGAAGA GCCACACTAC 
ATTGAGATTT GGAAAAAGCA CCTGGAAGCG TACAGTCAGC GTGCCCTGGA GCTGGAAGAC 
TCACTGGAGG CGTCAACCTC TCAGATGATG AACTTGAATT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Secisbp2 ( XM_006516964.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517027.1 Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X5, mRNA.

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CloneID	OMu48571
Clone ID Related Accession (Same CDS sequence)	XM_006516964.3
Accession Version	XM_006516964.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	selenocysteine insertion sequence-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516964.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCGGCCGCG GGAGCCGGAC GGCGAGGATA GCATCAAGTT GTCAGCTGAT 
GTCAAACCAT TCGTCCCTAA GTTTGCTGGG CTCAACGTGG CGTGGTCAGA GTCCTCAGAG 
ACACGTGTCT TCCCAGGCTG TGCGGCCACC TACTATCCAT TTGTACAGGA ACCACCAGCA 
GCTGAACAGA AAATGTATCC CGAAGACATG GCTTTCGGAG CCCCCACCTT TCCAGCACAG 
TACGTGTCTT CTGAGATAGC GCTGCATCCT TTTGCCTATC CCACTTACAC CCTAGAGTCC 
GCACAGAGTG TTTGCTCAGT GCCAACCCTG CAGTACGACT ACAGCCAAGC ACGGTGTCAC 
CCAGGCTTTC GGACAGCAAA GCCCCGGCAT GAGCACGTGT GCCCTCCACC TCAGGAAGCA 
AAAGGTGTAT TTAAGAAAAA ACCCTCTGAT GAGAGAAGAG CATGTGAAGA GCAAAAGTCA 
AGCAGCAGAA GGGCTGACAA TGCGGTGCCC TGTGAGGCGA GACCAGCCAG GGGGTCCAGT 
CACCTGTCCT CTCGAACTGA GAGCAGTTTG AAATCTGATG GTTACCACAA GCGACCCGAC 
CGCAAGTCCA GAATCCTTGC GAAGAGTGCA TCTACCTCTA AACCTGAATT TGAGTTTAGT 
AGGTTAGACT TTCCTGAACT GCAGAGTCCA AAGAACAGTA ACATGCCAGA GACACAGAAG 
CCGCCCAGGT GGGGGCCTCT TGGCCCTGCT GCCAGTAACA TGCCTCTCCT AGGAGACGTC 
GGCAAGCCCG TCGCAGATAT GGTAGAGGGC AAAATGGTGA AGAGCGATCA CACTGATGGA 
GCTGTGACCA GTAATGCCAC TACCAGTTCC CCTTCATGTA CCCAAGAGTT GTCTTGGACA 
CCAATGGGTT ATATTGTTCG GCAGACAGTG TCTTCAGATT CAGCAGCAGC CACTGAAAAT 
GTGACTTCCA TGATAAACCT AAAGAAGACT ACTTCATCAG CTGATGCTAA AAATGTTAGT 
GTGACATCTG AGGCTTTATC TTCAAATCCT TCCTACAACA GAGAAAAGCG TGTTTATCCT 
GCTCCAAAGG CCAAAGCATC ACAAGGAGGT GAACTTGAAC AAAACGAAAG CTCCAAAAAG 
AATAAGAAAA AGAAAGAGAA GTCTAAACCG AGTTATGAAG TCCTGACGGT TCAGGAGCCG 
CCAAGGATTG AAGATGCAGA GGAATTCCCC AACCTGTCAG TTGCGTCGGA AAGAAGACAC 
AGAGGGCAAT CACCGAAGCT TCACAGTAAA CAGCAGACGC AGAATGAATT TAAAACAAGT 
GGGAAGAAGA GCCAGGTCCC AGTGCAGCTG GACCTGGGGG GCATGCTGGC CGCGCTGGAG 
AAGCAGCAGC AGCAGCAGCA CGCCTCGCAC GCCAAGCCAT CCTCCAGACC CGTCGTGTTC 
TCAGTTGGAG CAGTGCCAGT CCTGTCCAAG GATGCCTCCT CCAGTGAGAG GGGACGCCGC 
TCCAGTCAGA TGAAGACCCC ACACAACCCC CTGGACTCCA GTGCCCCCCT GATGAAGAAG 
GGGAAGCAGA GGGAGATACC TAAGGCCAAG AAGCCCACCT CACTGAAGAA GATAATTTTG 
AAAGAACGGC AAGAGAGGAT GCAGCAGCGA CTCCAAGAAA GTGCTGTGAG CCTGACGGTG 
GCCAGTGATG ACTCACAGGA TGTGGAGAGT GGCGCCAGTA ACCAAACCCC CAGTCAGGAC 
AACCCCACAG GTCCAGAGAA GACAGAAGAA TCAGTGTCTT CTACACCTGT GGTTGAGGCT 
ACTGCAGCCA GATGCTTAGT AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517027.1
CDS	104..1933
Translation	MASERPREPDGEDSIKLSADVKPFVPKFAGLNVAWSESSETRVFPGCAATYYPFVQEPPAAEQKMYPEDMAFGAPTFPAQYVSSEIALHPFAYPTYTLESAQSVCSVPTLQYDYSQARCHPGFRTAKPRHEHVCPPPQEAKGVFKKKPSDERRACEEQKSSSRRADNAVPCEARPARGSSHLSSRTESSLKSDGYHKRPDRKSRILAKSASTSKPEFEFSRLDFPELQSPKNSNMPETQKPPRWGPLGPAASNMPLLGDVGKPVADMVEGKMVKSDHTDGAVTSNATTSSPSCTQELSWTPMGYIVRQTVSSDSAAATENVTSMINLKKTTSSADAKNVSVTSEALSSNPSYNREKRVYPAPKAKASQGGELEQNESSKKNKKKKEKSKPSYEVLTVQEPPRIEDAEEFPNLSVASERRHRGQSPKLHSKQQTQNEFKTSGKKSQVPVQLDLGGMLAALEKQQQQQHASHAKPSSRPVVFSVGAVPVLSKDASSSERGRRSSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERMQQRLQESAVSLTVASDDSQDVESGASNQTPSQDNPTGPEKTEESVSSTPVVEATAARCLVKK

Target ORF information:

RefSeq Version	XM_006516964.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516964.3

ORF Insert Sequence:

ATGGCGTCGG AGCGGCCGCG GGAGCCGGAC GGCGAGGATA GCATCAAGTT GTCAGCTGAT 
GTCAAACCAT TCGTCCCTAA GTTTGCTGGG CTCAACGTGG CGTGGTCAGA GTCCTCAGAG 
ACACGTGTCT TCCCAGGCTG TGCGGCCACC TACTATCCAT TTGTACAGGA ACCACCAGCA 
GCTGAACAGA AAATGTATCC CGAAGACATG GCTTTCGGAG CCCCCACCTT TCCAGCACAG 
TACGTGTCTT CTGAGATAGC GCTGCATCCT TTTGCCTATC CCACTTACAC CCTAGAGTCC 
GCACAGAGTG TTTGCTCAGT GCCAACCCTG CAGTACGACT ACAGCCAAGC ACGGTGTCAC 
CCAGGCTTTC GGACAGCAAA GCCCCGGCAT GAGCACGTGT GCCCTCCACC TCAGGAAGCA 
AAAGGTGTAT TTAAGAAAAA ACCCTCTGAT GAGAGAAGAG CATGTGAAGA GCAAAAGTCA 
AGCAGCAGAA GGGCTGACAA TGCGGTGCCC TGTGAGGCGA GACCAGCCAG GGGGTCCAGT 
CACCTGTCCT CTCGAACTGA GAGCAGTTTG AAATCTGATG GTTACCACAA GCGACCCGAC 
CGCAAGTCCA GAATCCTTGC GAAGAGTGCA TCTACCTCTA AACCTGAATT TGAGTTTAGT 
AGGTTAGACT TTCCTGAACT GCAGAGTCCA AAGAACAGTA ACATGCCAGA GACACAGAAG 
CCGCCCAGGT GGGGGCCTCT TGGCCCTGCT GCCAGTAACA TGCCTCTCCT AGGAGACGTC 
GGCAAGCCCG TCGCAGATAT GGTAGAGGGC AAAATGGTGA AGAGCGATCA CACTGATGGA 
GCTGTGACCA GTAATGCCAC TACCAGTTCC CCTTCATGTA CCCAAGAGTT GTCTTGGACA 
CCAATGGGTT ATATTGTTCG GCAGACAGTG TCTTCAGATT CAGCAGCAGC CACTGAAAAT 
GTGACTTCCA TGATAAACCT AAAGAAGACT ACTTCATCAG CTGATGCTAA AAATGTTAGT 
GTGACATCTG AGGCTTTATC TTCAAATCCT TCCTACAACA GAGAAAAGCG TGTTTATCCT 
GCTCCAAAGG CCAAAGCATC ACAAGGAGGT GAACTTGAAC AAAACGAAAG CTCCAAAAAG 
AATAAGAAAA AGAAAGAGAA GTCTAAACCG AGTTATGAAG TCCTGACGGT TCAGGAGCCG 
CCAAGGATTG AAGATGCAGA GGAATTCCCC AACCTGTCAG TTGCGTCGGA AAGAAGACAC 
AGAGGGCAAT CACCGAAGCT TCACAGTAAA CAGCAGACGC AGAATGAATT TAAAACAAGT 
GGGAAGAAGA GCCAGGTCCC AGTGCAGCTG GACCTGGGGG GCATGCTGGC CGCGCTGGAG 
AAGCAGCAGC AGCAGCAGCA CGCCTCGCAC GCCAAGCCAT CCTCCAGACC CGTCGTGTTC 
TCAGTTGGAG CAGTGCCAGT CCTGTCCAAG GATGCCTCCT CCAGTGAGAG GGGACGCCGC 
TCCAGTCAGA TGAAGACCCC ACACAACCCC CTGGACTCCA GTGCCCCCCT GATGAAGAAG 
GGGAAGCAGA GGGAGATACC TAAGGCCAAG AAGCCCACCT CACTGAAGAA GATAATTTTG 
AAAGAACGGC AAGAGAGGAT GCAGCAGCGA CTCCAAGAAA GTGCTGTGAG CCTGACGGTG 
GCCAGTGATG ACTCACAGGA TGTGGAGAGT GGCGCCAGTA ACCAAACCCC CAGTCAGGAC 
AACCCCACAG GTCCAGAGAA GACAGAAGAA TCAGTGTCTT CTACACCTGT GGTTGAGGCT 
ACTGCAGCCA GATGCTTAGT AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Secisbp2 ( XM_006516965.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517028.1 Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu48572
Clone ID Related Accession (Same CDS sequence)	XM_006516965.3
Accession Version	XM_006516965.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	selenocysteine insertion sequence-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG AGCGGCCGCG GGAGCCGGAC GGCGAGGATA GCATCAAGTT GTCAGCTGAT 
GTCAAACCAT TCGTCCCTAA GTTTGCTGGG CTCAACGTGG CGTGGTCAGA GTCCTCAGAG 
ACACGTGTCT TCCCAGGCTG TGCGGCCACC TACTATCCAT TTGTACAGGA ACCACCAGCA 
GCTGAACAGA AAATGTATCC CGAAGACATG GCTTTCGGAG CCCCCACCTT TCCAGCACAG 
TACGTGTCTT CTGAGATAGC GCTGCATCCT TTTGCCTATC CCACTTACAC CCTAGAGTCC 
GCACAGAGTG TTTGCTCAGT GCCAACCCTG CAGTACGACT ACAGCCAAGC ACGGTGTCAC 
CCAGGCTTTC GGACAGCAAA GCCCCGGCAT GAGCACGTGT GCCCTCCACC TCAGGAAGCA 
AAAGGTGTAT TTAAGAAAAA ACCCTCTGAT GAGAGAAGAG CATGTGAAGA GCAAAAGTCA 
AGCAGCAGAA GGGCTGACAA TGCGGTGCCC TGTGAGGCGA GACCAGCCAG GGGGTCCAGT 
CACCTGTCCT CTCGAACTGA GAGCAGTTTG AAATCTGATG GTTACCACAA GCGACCCGAC 
CGCAAGTCCA GAATCCTTGC GAAGAGTGCA TCTACCTCTA AACCTGAATT TGAGTTTAGT 
AGGTTAGACT TTCCTGAACT GCAGAGTCCA AAGAACAGTA ACATGCCAGA GACACAGAAG 
CCGCCCAGGT GGGGGCCTCT TGGCCCTGCT GCCAGTAACA TGCCTCTCCT AGGAGACGTC 
GGCAAGCCCG TCGCAGATAT GGTAGAGGGC AAAATGGTGA AGAGCGATCA CACTGATGGA 
GCTGTGACCA GTAATGCCAC TACCAGTTCC CCTTCATGTA CCCAAGAGTT GTCTTGGACA 
CCAATGGGTT ATATTGTTCG GCAGACAGTG TCTTCAGATT CAGCAGCAGC CACTGAAAAT 
GTGACTTCCA TGATAAACCT AAAGAAGACT ACTTCATCAG CTGATGCTAA AAATGTTAGT 
GTGACATCTG AGGCTTTATC TTCAAATCCT TCCTACAACA GAGAAAAGCG TGTTTATCCT 
GCTCCAAAGG CCAAAGCATC ACAAGGAGGT GAACTTGAAC AAAACGAAAG CTCCAAAAAG 
AATAAGAAAA AGAAAGAGAA GTCTAAACCG AGTTATGAAG TCCTGACGGT TCAGGAGCCG 
CCAAGGATTG AAGATGCAGA GGAATTCCCC AACCTGTCAG TTGCGTCGGA AAGAAGACAC 
AGAGGGCAAT CACCGAAGCT TCACAGTAAA CAGCAGACGC AGAATGAATT TAAAACAAGT 
GGGAAGAAGA GCCAGGTCCC AGTGCAGCTG GACCTGGGGG GCATGCTGGC CGCGCTGGAG 
AAGCAGCAGC AGCAGCAGCA CGCCTCGCAC GCCAAGCCAT CCTCCAGACC CGTCGTGTTC 
TCAGTTGGAG CAGTGCCAGT CCTGTCCAAG GATGCCTCCT CCAGTGAGAG GGGACGCCGC 
TCCAGTCAGA TGAAGACCCC ACACAACCCC CTGGACTCCA GTGCCCCCCT GATGAAGAAG 
GGGAAGCAGA GGGAGATACC TAAGGCCAAG AAGCCCACCT CACTGAAGAA GATAATTTTG 
AAAGAACGGC AAGAGAGGAT GCAGCAGCGA CTCCAAGAAA GTGCTGTGAG CCTGACGGTG 
GCCAGTGATG ACTCACAGGA TGTGGAGAGT GGCGCCAGTA ACCAAACCCC CAGTCAGGAC 
AACCCCACAG CTACTGCAGC CAGATGCTTA GTAAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517028.1
CDS	103..1884
Translation	MASERPREPDGEDSIKLSADVKPFVPKFAGLNVAWSESSETRVFPGCAATYYPFVQEPPAAEQKMYPEDMAFGAPTFPAQYVSSEIALHPFAYPTYTLESAQSVCSVPTLQYDYSQARCHPGFRTAKPRHEHVCPPPQEAKGVFKKKPSDERRACEEQKSSSRRADNAVPCEARPARGSSHLSSRTESSLKSDGYHKRPDRKSRILAKSASTSKPEFEFSRLDFPELQSPKNSNMPETQKPPRWGPLGPAASNMPLLGDVGKPVADMVEGKMVKSDHTDGAVTSNATTSSPSCTQELSWTPMGYIVRQTVSSDSAAATENVTSMINLKKTTSSADAKNVSVTSEALSSNPSYNREKRVYPAPKAKASQGGELEQNESSKKNKKKKEKSKPSYEVLTVQEPPRIEDAEEFPNLSVASERRHRGQSPKLHSKQQTQNEFKTSGKKSQVPVQLDLGGMLAALEKQQQQQHASHAKPSSRPVVFSVGAVPVLSKDASSSERGRRSSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERMQQRLQESAVSLTVASDDSQDVESGASNQTPSQDNPTATAARCLVKK

Target ORF information:

RefSeq Version	XM_006516965.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516965.3

ORF Insert Sequence:

ATGGCGTCGG AGCGGCCGCG GGAGCCGGAC GGCGAGGATA GCATCAAGTT GTCAGCTGAT 
GTCAAACCAT TCGTCCCTAA GTTTGCTGGG CTCAACGTGG CGTGGTCAGA GTCCTCAGAG 
ACACGTGTCT TCCCAGGCTG TGCGGCCACC TACTATCCAT TTGTACAGGA ACCACCAGCA 
GCTGAACAGA AAATGTATCC CGAAGACATG GCTTTCGGAG CCCCCACCTT TCCAGCACAG 
TACGTGTCTT CTGAGATAGC GCTGCATCCT TTTGCCTATC CCACTTACAC CCTAGAGTCC 
GCACAGAGTG TTTGCTCAGT GCCAACCCTG CAGTACGACT ACAGCCAAGC ACGGTGTCAC 
CCAGGCTTTC GGACAGCAAA GCCCCGGCAT GAGCACGTGT GCCCTCCACC TCAGGAAGCA 
AAAGGTGTAT TTAAGAAAAA ACCCTCTGAT GAGAGAAGAG CATGTGAAGA GCAAAAGTCA 
AGCAGCAGAA GGGCTGACAA TGCGGTGCCC TGTGAGGCGA GACCAGCCAG GGGGTCCAGT 
CACCTGTCCT CTCGAACTGA GAGCAGTTTG AAATCTGATG GTTACCACAA GCGACCCGAC 
CGCAAGTCCA GAATCCTTGC GAAGAGTGCA TCTACCTCTA AACCTGAATT TGAGTTTAGT 
AGGTTAGACT TTCCTGAACT GCAGAGTCCA AAGAACAGTA ACATGCCAGA GACACAGAAG 
CCGCCCAGGT GGGGGCCTCT TGGCCCTGCT GCCAGTAACA TGCCTCTCCT AGGAGACGTC 
GGCAAGCCCG TCGCAGATAT GGTAGAGGGC AAAATGGTGA AGAGCGATCA CACTGATGGA 
GCTGTGACCA GTAATGCCAC TACCAGTTCC CCTTCATGTA CCCAAGAGTT GTCTTGGACA 
CCAATGGGTT ATATTGTTCG GCAGACAGTG TCTTCAGATT CAGCAGCAGC CACTGAAAAT 
GTGACTTCCA TGATAAACCT AAAGAAGACT ACTTCATCAG CTGATGCTAA AAATGTTAGT 
GTGACATCTG AGGCTTTATC TTCAAATCCT TCCTACAACA GAGAAAAGCG TGTTTATCCT 
GCTCCAAAGG CCAAAGCATC ACAAGGAGGT GAACTTGAAC AAAACGAAAG CTCCAAAAAG 
AATAAGAAAA AGAAAGAGAA GTCTAAACCG AGTTATGAAG TCCTGACGGT TCAGGAGCCG 
CCAAGGATTG AAGATGCAGA GGAATTCCCC AACCTGTCAG TTGCGTCGGA AAGAAGACAC 
AGAGGGCAAT CACCGAAGCT TCACAGTAAA CAGCAGACGC AGAATGAATT TAAAACAAGT 
GGGAAGAAGA GCCAGGTCCC AGTGCAGCTG GACCTGGGGG GCATGCTGGC CGCGCTGGAG 
AAGCAGCAGC AGCAGCAGCA CGCCTCGCAC GCCAAGCCAT CCTCCAGACC CGTCGTGTTC 
TCAGTTGGAG CAGTGCCAGT CCTGTCCAAG GATGCCTCCT CCAGTGAGAG GGGACGCCGC 
TCCAGTCAGA TGAAGACCCC ACACAACCCC CTGGACTCCA GTGCCCCCCT GATGAAGAAG 
GGGAAGCAGA GGGAGATACC TAAGGCCAAG AAGCCCACCT CACTGAAGAA GATAATTTTG 
AAAGAACGGC AAGAGAGGAT GCAGCAGCGA CTCCAAGAAA GTGCTGTGAG CCTGACGGTG 
GCCAGTGATG ACTCACAGGA TGTGGAGAGT GGCGCCAGTA ACCAAACCCC CAGTCAGGAC 
AACCCCACAG CTACTGCAGC CAGATGCTTA GTAAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Secisbp2 ( XM_006516966.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517029.1 Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu48573
Clone ID Related Accession (Same CDS sequence)	XM_006516966.1 , XM_006516966.2
Accession Version	XM_006516966.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	selenocysteine insertion sequence-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTAC CAGTTCCCCT TCATGTACCC AAGACAGTGT CTTCAGATTC AGCAGCAGCC 
ACTGAAAATG TGACTTCCAT GATAAACCTA AAGAAGACTA CTTCATCAGC TGATGCTAAA 
AATGTTAGTG TGACATCTGA GGCTTTATCT TCAAATCCTT CCTACAACAG AGAAAAGCGT 
GTTTATCCTG CTCCAAAGGC CAAAGCATCA CAAGGAGGTG AACTTGAACA AAACGAAAGC 
TCCAAAAAGA ATAAGAAAAA GAAAGAGAAG TCTAAACCGA GTTATGAAGT CCTGACGGTT 
CAGGAGCCGC CAAGGATTGA AGATGCAGAG GAATTCCCCA ACCTGTCAGT TGCGTCGGAA 
AGAAGACACA GAGGGCAATC ACCGAAGCTT CACAGTAAAC AGCAGACGCA GAATGAATTT 
AAAACAAGTG GGAAGAAGAG CCAGGTCCCA GTGCAGCTGG ACCTGGGGGG CATGCTGGCC 
GCGCTGGAGA AGCAGCAGCA GCAGCAGCAC GCCTCGCACG CCAAGCCATC CTCCAGACCC 
GTCGTGTTCT CAGTTGGAGC AGTGCCAGTC CTGTCCAAGG ATGCCTCCTC CAGTGAGAGG 
GGACGCCGCT CCAGTCAGAT GAAGACCCCA CACAACCCCC TGGACTCCAG TGCCCCCCTG 
ATGAAGAAGG GGAAGCAGAG GGAGATACCT AAGGCCAAGA AGCCCACCTC ACTGAAGAAG 
ATAATTTTGA AAGAACGGCA AGAGAGGATG CAGCAGCGAC TCCAAGAAAG TGCTGTGAGC 
CTGACGGTGG CCAGTGATGA CTCACAGGAT GTGGAGAGTG GCGCCAGTAA CCAAACCCCC 
AGTCAGGACA ACCCCACAGG TCCAGAGAAG ACAGAAGAAT CAGTGTCTTC TACACCTGTG 
GTTGAGGGTG AGTCAGAGGA GCCAGCTGGC ACAGAGTTCC AGAGGGACCC AGAGGCTTGC 
CAGCCTGCCC CTGACAGTGC CACCTTCCCC AAGATCCACA GCCGGAGGTT CCGGGACTAC 
TGCAGCCAGA TGCTTAGTAA AGAAGTAGAT GCTTGTGTCA CGGGTCTGCT CAAGGAGCTG 
GTGCGCTTCC AAGACCGCAT GTACCAGAAG GATCCCGTCA AGGCCAAGAC AAAACGGCGG 
CTCGTGCTGG GGCTGAGGGA GGTCCTGAAA CACCTGAAGC TCAGGAAGCT GAAGTGCATC 
ATCATCTCTC CCAACTGTGA GAAGACCCAG TCTAAAGGTG GGCTGGACGA CACGCTGCAC 
ACCATCATCG ACTGCGCCTG CGAGCAGAAC ATCCCCTTCG TGTTTGCACT CAATCGCAAG 
GCTCTGGGGC GGAGCCTGAA TAAAGCTGTT CCTGTCAGCA TTGTAGGGAT CTTCAGCTAC 
GATGGGGCCC AGGACCAGTT CCACAAGATG GTTGAGCTGA CCATGGCAGC CCGTCAGGCA 
TACAAGACCA TGTTGGAGAC AATGCGGCAG GAACAGGCAG GAGAACCTGG ACCTCAGTCC 
CCTCCCAGCC CACCCATGCA AGACCCCATC CCATCCACGG AAGAAGGCAC CCTCCCTTCC 
ACTGGAGAAG AGCCACACTA CATTGAGATT TGGAAAAAGC ACCTGGAAGC GTACAGTCAG 
CGTGCCCTGG AGCTGGAAGA CTCACTGGAG GCGTCAACCT CTCAGATGAT GAACTTGAAT 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517029.1
CDS	800..2485
Translation	MPLPVPLHVPKTVSSDSAAATENVTSMINLKKTTSSADAKNVSVTSEALSSNPSYNREKRVYPAPKAKASQGGELEQNESSKKNKKKKEKSKPSYEVLTVQEPPRIEDAEEFPNLSVASERRHRGQSPKLHSKQQTQNEFKTSGKKSQVPVQLDLGGMLAALEKQQQQQHASHAKPSSRPVVFSVGAVPVLSKDASSSERGRRSSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERMQQRLQESAVSLTVASDDSQDVESGASNQTPSQDNPTGPEKTEESVSSTPVVEGESEEPAGTEFQRDPEACQPAPDSATFPKIHSRRFRDYCSQMLSKEVDACVTGLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKTQSKGGLDDTLHTIIDCACEQNIPFVFALNRKALGRSLNKAVPVSIVGIFSYDGAQDQFHKMVELTMAARQAYKTMLETMRQEQAGEPGPQSPPSPPMQDPIPSTEEGTLPSTGEEPHYIEIWKKHLEAYSQRALELEDSLEASTSQMMNLNL

Target ORF information:

RefSeq Version	XM_006516966.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516966.1

ORF Insert Sequence:

ATGCCACTAC CAGTTCCCCT TCATGTACCC AAGACAGTGT CTTCAGATTC AGCAGCAGCC 
ACTGAAAATG TGACTTCCAT GATAAACCTA AAGAAGACTA CTTCATCAGC TGATGCTAAA 
AATGTTAGTG TGACATCTGA GGCTTTATCT TCAAATCCTT CCTACAACAG AGAAAAGCGT 
GTTTATCCTG CTCCAAAGGC CAAAGCATCA CAAGGAGGTG AACTTGAACA AAACGAAAGC 
TCCAAAAAGA ATAAGAAAAA GAAAGAGAAG TCTAAACCGA GTTATGAAGT CCTGACGGTT 
CAGGAGCCGC CAAGGATTGA AGATGCAGAG GAATTCCCCA ACCTGTCAGT TGCGTCGGAA 
AGAAGACACA GAGGGCAATC ACCGAAGCTT CACAGTAAAC AGCAGACGCA GAATGAATTT 
AAAACAAGTG GGAAGAAGAG CCAGGTCCCA GTGCAGCTGG ACCTGGGGGG CATGCTGGCC 
GCGCTGGAGA AGCAGCAGCA GCAGCAGCAC GCCTCGCACG CCAAGCCATC CTCCAGACCC 
GTCGTGTTCT CAGTTGGAGC AGTGCCAGTC CTGTCCAAGG ATGCCTCCTC CAGTGAGAGG 
GGACGCCGCT CCAGTCAGAT GAAGACCCCA CACAACCCCC TGGACTCCAG TGCCCCCCTG 
ATGAAGAAGG GGAAGCAGAG GGAGATACCT AAGGCCAAGA AGCCCACCTC ACTGAAGAAG 
ATAATTTTGA AAGAACGGCA AGAGAGGATG CAGCAGCGAC TCCAAGAAAG TGCTGTGAGC 
CTGACGGTGG CCAGTGATGA CTCACAGGAT GTGGAGAGTG GCGCCAGTAA CCAAACCCCC 
AGTCAGGACA ACCCCACAGG TCCAGAGAAG ACAGAAGAAT CAGTGTCTTC TACACCTGTG 
GTTGAGGGTG AGTCAGAGGA GCCAGCTGGC ACAGAGTTCC AGAGGGACCC AGAGGCTTGC 
CAGCCTGCCC CTGACAGTGC CACCTTCCCC AAGATCCACA GCCGGAGGTT CCGGGACTAC 
TGCAGCCAGA TGCTTAGTAA AGAAGTAGAT GCTTGTGTCA CGGGTCTGCT CAAGGAGCTG 
GTGCGCTTCC AAGACCGCAT GTACCAGAAG GATCCCGTCA AGGCCAAGAC AAAACGGCGG 
CTCGTGCTGG GGCTGAGGGA GGTCCTGAAA CACCTGAAGC TCAGGAAGCT GAAGTGCATC 
ATCATCTCTC CCAACTGTGA GAAGACCCAG TCTAAAGGTG GGCTGGACGA CACGCTGCAC 
ACCATCATCG ACTGCGCCTG CGAGCAGAAC ATCCCCTTCG TGTTTGCACT CAATCGCAAG 
GCTCTGGGGC GGAGCCTGAA TAAAGCTGTT CCTGTCAGCA TTGTAGGGAT CTTCAGCTAC 
GATGGGGCCC AGGACCAGTT CCACAAGATG GTTGAGCTGA CCATGGCAGC CCGTCAGGCA 
TACAAGACCA TGTTGGAGAC AATGCGGCAG GAACAGGCAG GAGAACCTGG ACCTCAGTCC 
CCTCCCAGCC CACCCATGCA AGACCCCATC CCATCCACGG AAGAAGGCAC CCTCCCTTCC 
ACTGGAGAAG AGCCACACTA CATTGAGATT TGGAAAAAGC ACCTGGAAGC GTACAGTCAG 
CGTGCCCTGG AGCTGGAAGA CTCACTGGAG GCGTCAACCT CTCAGATGAT GAACTTGAAT 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Secisbp2 ( XM_006516966.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517029.1 Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X7, mRNA.

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CloneID	OMu48573
Clone ID Related Accession (Same CDS sequence)	XM_006516966.1 , XM_006516966.2
Accession Version	XM_006516966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	selenocysteine insertion sequence-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTAC CAGTTCCCCT TCATGTACCC AAGACAGTGT CTTCAGATTC AGCAGCAGCC 
ACTGAAAATG TGACTTCCAT GATAAACCTA AAGAAGACTA CTTCATCAGC TGATGCTAAA 
AATGTTAGTG TGACATCTGA GGCTTTATCT TCAAATCCTT CCTACAACAG AGAAAAGCGT 
GTTTATCCTG CTCCAAAGGC CAAAGCATCA CAAGGAGGTG AACTTGAACA AAACGAAAGC 
TCCAAAAAGA ATAAGAAAAA GAAAGAGAAG TCTAAACCGA GTTATGAAGT CCTGACGGTT 
CAGGAGCCGC CAAGGATTGA AGATGCAGAG GAATTCCCCA ACCTGTCAGT TGCGTCGGAA 
AGAAGACACA GAGGGCAATC ACCGAAGCTT CACAGTAAAC AGCAGACGCA GAATGAATTT 
AAAACAAGTG GGAAGAAGAG CCAGGTCCCA GTGCAGCTGG ACCTGGGGGG CATGCTGGCC 
GCGCTGGAGA AGCAGCAGCA GCAGCAGCAC GCCTCGCACG CCAAGCCATC CTCCAGACCC 
GTCGTGTTCT CAGTTGGAGC AGTGCCAGTC CTGTCCAAGG ATGCCTCCTC CAGTGAGAGG 
GGACGCCGCT CCAGTCAGAT GAAGACCCCA CACAACCCCC TGGACTCCAG TGCCCCCCTG 
ATGAAGAAGG GGAAGCAGAG GGAGATACCT AAGGCCAAGA AGCCCACCTC ACTGAAGAAG 
ATAATTTTGA AAGAACGGCA AGAGAGGATG CAGCAGCGAC TCCAAGAAAG TGCTGTGAGC 
CTGACGGTGG CCAGTGATGA CTCACAGGAT GTGGAGAGTG GCGCCAGTAA CCAAACCCCC 
AGTCAGGACA ACCCCACAGG TCCAGAGAAG ACAGAAGAAT CAGTGTCTTC TACACCTGTG 
GTTGAGGGTG AGTCAGAGGA GCCAGCTGGC ACAGAGTTCC AGAGGGACCC AGAGGCTTGC 
CAGCCTGCCC CTGACAGTGC CACCTTCCCC AAGATCCACA GCCGGAGGTT CCGGGACTAC 
TGCAGCCAGA TGCTTAGTAA AGAAGTAGAT GCTTGTGTCA CGGGTCTGCT CAAGGAGCTG 
GTGCGCTTCC AAGACCGCAT GTACCAGAAG GATCCCGTCA AGGCCAAGAC AAAACGGCGG 
CTCGTGCTGG GGCTGAGGGA GGTCCTGAAA CACCTGAAGC TCAGGAAGCT GAAGTGCATC 
ATCATCTCTC CCAACTGTGA GAAGACCCAG TCTAAAGGTG GGCTGGACGA CACGCTGCAC 
ACCATCATCG ACTGCGCCTG CGAGCAGAAC ATCCCCTTCG TGTTTGCACT CAATCGCAAG 
GCTCTGGGGC GGAGCCTGAA TAAAGCTGTT CCTGTCAGCA TTGTAGGGAT CTTCAGCTAC 
GATGGGGCCC AGGACCAGTT CCACAAGATG GTTGAGCTGA CCATGGCAGC CCGTCAGGCA 
TACAAGACCA TGTTGGAGAC AATGCGGCAG GAACAGGCAG GAGAACCTGG ACCTCAGTCC 
CCTCCCAGCC CACCCATGCA AGACCCCATC CCATCCACGG AAGAAGGCAC CCTCCCTTCC 
ACTGGAGAAG AGCCACACTA CATTGAGATT TGGAAAAAGC ACCTGGAAGC GTACAGTCAG 
CGTGCCCTGG AGCTGGAAGA CTCACTGGAG GCGTCAACCT CTCAGATGAT GAACTTGAAT 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517029.1
CDS	934..2619
Translation	MPLPVPLHVPKTVSSDSAAATENVTSMINLKKTTSSADAKNVSVTSEALSSNPSYNREKRVYPAPKAKASQGGELEQNESSKKNKKKKEKSKPSYEVLTVQEPPRIEDAEEFPNLSVASERRHRGQSPKLHSKQQTQNEFKTSGKKSQVPVQLDLGGMLAALEKQQQQQHASHAKPSSRPVVFSVGAVPVLSKDASSSERGRRSSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERMQQRLQESAVSLTVASDDSQDVESGASNQTPSQDNPTGPEKTEESVSSTPVVEGESEEPAGTEFQRDPEACQPAPDSATFPKIHSRRFRDYCSQMLSKEVDACVTGLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKTQSKGGLDDTLHTIIDCACEQNIPFVFALNRKALGRSLNKAVPVSIVGIFSYDGAQDQFHKMVELTMAARQAYKTMLETMRQEQAGEPGPQSPPSPPMQDPIPSTEEGTLPSTGEEPHYIEIWKKHLEAYSQRALELEDSLEASTSQMMNLNL

Target ORF information:

RefSeq Version	XM_006516966.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SECIS binding protein 2 (Secisbp2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516966.2

ORF Insert Sequence:

ATGCCACTAC CAGTTCCCCT TCATGTACCC AAGACAGTGT CTTCAGATTC AGCAGCAGCC 
ACTGAAAATG TGACTTCCAT GATAAACCTA AAGAAGACTA CTTCATCAGC TGATGCTAAA 
AATGTTAGTG TGACATCTGA GGCTTTATCT TCAAATCCTT CCTACAACAG AGAAAAGCGT 
GTTTATCCTG CTCCAAAGGC CAAAGCATCA CAAGGAGGTG AACTTGAACA AAACGAAAGC 
TCCAAAAAGA ATAAGAAAAA GAAAGAGAAG TCTAAACCGA GTTATGAAGT CCTGACGGTT 
CAGGAGCCGC CAAGGATTGA AGATGCAGAG GAATTCCCCA ACCTGTCAGT TGCGTCGGAA 
AGAAGACACA GAGGGCAATC ACCGAAGCTT CACAGTAAAC AGCAGACGCA GAATGAATTT 
AAAACAAGTG GGAAGAAGAG CCAGGTCCCA GTGCAGCTGG ACCTGGGGGG CATGCTGGCC 
GCGCTGGAGA AGCAGCAGCA GCAGCAGCAC GCCTCGCACG CCAAGCCATC CTCCAGACCC 
GTCGTGTTCT CAGTTGGAGC AGTGCCAGTC CTGTCCAAGG ATGCCTCCTC CAGTGAGAGG 
GGACGCCGCT CCAGTCAGAT GAAGACCCCA CACAACCCCC TGGACTCCAG TGCCCCCCTG 
ATGAAGAAGG GGAAGCAGAG GGAGATACCT AAGGCCAAGA AGCCCACCTC ACTGAAGAAG 
ATAATTTTGA AAGAACGGCA AGAGAGGATG CAGCAGCGAC TCCAAGAAAG TGCTGTGAGC 
CTGACGGTGG CCAGTGATGA CTCACAGGAT GTGGAGAGTG GCGCCAGTAA CCAAACCCCC 
AGTCAGGACA ACCCCACAGG TCCAGAGAAG ACAGAAGAAT CAGTGTCTTC TACACCTGTG 
GTTGAGGGTG AGTCAGAGGA GCCAGCTGGC ACAGAGTTCC AGAGGGACCC AGAGGCTTGC 
CAGCCTGCCC CTGACAGTGC CACCTTCCCC AAGATCCACA GCCGGAGGTT CCGGGACTAC 
TGCAGCCAGA TGCTTAGTAA AGAAGTAGAT GCTTGTGTCA CGGGTCTGCT CAAGGAGCTG 
GTGCGCTTCC AAGACCGCAT GTACCAGAAG GATCCCGTCA AGGCCAAGAC AAAACGGCGG 
CTCGTGCTGG GGCTGAGGGA GGTCCTGAAA CACCTGAAGC TCAGGAAGCT GAAGTGCATC 
ATCATCTCTC CCAACTGTGA GAAGACCCAG TCTAAAGGTG GGCTGGACGA CACGCTGCAC 
ACCATCATCG ACTGCGCCTG CGAGCAGAAC ATCCCCTTCG TGTTTGCACT CAATCGCAAG 
GCTCTGGGGC GGAGCCTGAA TAAAGCTGTT CCTGTCAGCA TTGTAGGGAT CTTCAGCTAC 
GATGGGGCCC AGGACCAGTT CCACAAGATG GTTGAGCTGA CCATGGCAGC CCGTCAGGCA 
TACAAGACCA TGTTGGAGAC AATGCGGCAG GAACAGGCAG GAGAACCTGG ACCTCAGTCC 
CCTCCCAGCC CACCCATGCA AGACCCCATC CCATCCACGG AAGAAGGCAC CCTCCCTTCC 
ACTGGAGAAG AGCCACACTA CATTGAGATT TGGAAAAAGC ACCTGGAAGC GTACAGTCAG 
CGTGCCCTGG AGCTGGAAGA CTCACTGGAG GCGTCAACCT CTCAGATGAT GAACTTGAAT 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.
Endocrinology158(12)4317-4330(2017 Dec)
Fu J,Fujisawa H,Follman B,Liao XH,Dumitrescu AM

The RNA-binding protein Secisbp2 differentially modulates UGA codon reassignment and RNA decay.
Nucleic acids research45(7)4094-4107(2017 Apr)
Fradejas-Villar N,Seeher S,Anderson CB,Doengi M,Carlson BA,Hatfield DL,Schweizer U,Howard MT

Secisbp2 is essential for embryonic development and enhances selenoprotein expression.
Antioxidants & redox signaling21(6)835-49(2014 Aug)
Seeher S,Atassi T,Mahdi Y,Carlson BA,Braun D,Wirth EK,Klein MO,Reix N,Miniard AC,Schomburg L,Hatfield DL,Driscoll DM,Schweizer U

Impaired selenoprotein expression in brain triggers striatal neuronal loss leading to co-ordination defects in mice.
The Biochemical journal462(1)67-75(2014 Aug)
Seeher S,Carlson BA,Miniard AC,Wirth EK,Mahdi Y,Hatfield DL,Driscoll DM,Schweizer U

A novel insight into the mechanism of mammalian selenoprotein synthesis.
RNA (New York, N.Y.)19(8)1147-58(2013 Aug)
Kossinova O,Malygin A,Krol A,Karpova G

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Secisbp2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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