Asxl2 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017315229.1	XP_017170718.1	putative Polycomb group protein ASXL2 isoform X1
XM_017315230.1	XP_017170719.1	putative Polycomb group protein ASXL2 isoform X3
XM_017315232.1	XP_017170721.1	putative Polycomb group protein ASXL2 isoform X4
XM_030246971.1	XP_030102831.1	putative Polycomb group protein ASXL2 isoform X5
NM_001270988.1	NP_001257917.1	putative Polycomb group protein ASXL2 isoform 1
XM_017315231.1	XP_017170720.1	putative Polycomb group protein ASXL2 isoform X4
XM_006515239.4	XP_006515302.1	putative Polycomb group protein ASXL2 isoform X2
XM_006515241.2	XP_006515304.1	putative Polycomb group protein ASXL2 isoform X6
XM_030246972.1	XP_030102832.1	putative Polycomb group protein ASXL2 isoform X7
XM_006515239.3	XP_006515302.1	putative Polycomb group protein ASXL2 isoform X2
XM_006515242.2	XP_006515305.1	putative Polycomb group protein ASXL2 isoform X6
XM_030246967.1	XP_030102827.1	putative Polycomb group protein ASXL2 isoform X1
XM_030246969.1	XP_030102829.1	putative Polycomb group protein ASXL2 isoform X4
XM_030246970.1	XP_030102830.1	putative Polycomb group protein ASXL2 isoform X5
NM_172421.5	NP_766009.2	putative Polycomb group protein ASXL2 isoform 2
XM_030246968.1	XP_030102828.1	putative Polycomb group protein ASXL2 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu47430	XM_006515239.4 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu47432	XM_030246972.1 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu71297	XM_030246970.1 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu71297	XM_030246971.1 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu78329	XM_017315229.1 Latest version!	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu78329	XM_030246967.1 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu78330	XM_017315230.1 Latest version!	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu78330	XM_030246968.1 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu78331	XM_017315232.1 Latest version!	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu78331	XM_017315231.1 Latest version!	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu86156	XM_030246969.1 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu47431	XM_006515241.2 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu47432	XM_006515242.2 Latest version!	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu03419	NM_172421.5 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu06891	NM_001270988.1 Latest version!	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu47430	XM_006515239.3	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

Asxl2 ( XM_006515239.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515302.1 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu47430
Clone ID Related Accession (Same CDS sequence)	XM_006515239.4 , XM_006515239.3
Accession Version	XM_006515239.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515239.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TGGGACTTCC CCTCTTGCAT GCTTGAATGC AATGCTGCAC 
ACAAACTCCC GAGGAGAAGA AGGCATCTTC TATAAAGTTC CAGGTAGAAT GGGCGTGTAT 
ACTTTGAAGA AAGATGTGCC AGATGGAGTG AAAGAGCTGT CAGAAGGTTC AGAAGAAAGC 
AGTGATGGTC AGTCAGATTC TCAGAGCTCT GAGAACAGCA GCAGCAGTGA TGGTGGCAGC 
AACAAAGAAG GGAGAAAGAG CAGGTGGAAA AGGAAAGTAT CATCTAGACT GTCACATCCA 
CCCTCTCCCC CGTCAGGCTG CCCATCACCC ACCATTCCAG CAAGTAAAGT CATTTCTCCA 
TCACAGAAGC ACAGCAAAAA GGCATTAAAA CAGGCCTTGA AGCAGCAACA ACAGAAGAAG 
AAGCAGCAGC AATGCAGACC AAGCATGTCC ATCTCCAATC AACACCTCTC TCTCAAGACT 
GTCAAAGCAG CCAGTGACTC TGTACCTGCC AAACCTGCCC TATGGGAAGG AAAGCAATCT 
GATGGACAGT CAAGCAGTCC CCAGAACTCA AATTCCAGCT TTTCTTCCTC AGTGAAAGTG 
GAAAGTTCTC TGCTAGGCTT GGGGAAGAAG TCTTTCCAGA GGTCTGACAG ACTCCACACA 
AGACAGATGA AAAGGACTAA ATGTGCTGAC ATTGATGTTG AGACTCCTGA TTCCATTCTG 
GTTAATACTA ATCTTCGAGC ACTGATCAAC AAGCACACCT TTTCAGTTCT TCCTGGAGAC 
TGCCAGCAGC GCCTGCTTTT GTTGCTTCCA GAGGTGGATC GACAGGTTGG TCCAGATGGC 
CTGATGAAGT TGAATGGCTC AGCCCTTAAC AATGAGTTCT TCACTTCTGC AGCCCAGGGC 
TGGAAGGAAA GGCTCTCAGA AGGTGAATTT ACTCCTGAAA TGCAGGTGAG AATTCGACAA 
GAGATTGAGA AGGAGAAGAA AGTAGAGCTG TGGAAGGAAC AATTCTTTGA AAATTACTAT 
GGTCAGAGTT CTGGCCTAAG CCTTGAAGAT TCACAGAAAC TGACAGCTTC CTCAAGTGAT 
CCCAAAGCAA AGAAAACCCC AGCTGAGCAA CCAAAATCCA TACTTCCTTC AGAGGCCTCT 
CCTGTCAGAA TTGTCCCAGT AGTTCCTCAG TCCGAGTGTA AAGAAGAAGC AGTGCAGATA 
CCATCACCAT CCCAAAAGGA AGAAAATCAA GACGAGGCGA GGCCAGACTC CAAATCCCCA 
GAACCTGTTC TTGCATCAGC TAGCAATACA AATGAGCTTA TCACCATGAA ACCCATCAAG 
AGCCCTAAGG ATGAGGGTCT CTTAGAGCAG AAGCCTGTTG CCTGTGCTGA ACAAGAATCT 
GAGAAAGAAA ATCATGTAAC CACAACTTCT CGTAATAACA AAAGTGAGAA CCAAGAAGCG 
TTAGCTATAT CCCCAAGCAA ATCTAAAAAT GCTGGGTTAC AAAAACCCAT AATAAAGCCT 
GTTGCAGAAG CCAGTCCACT GAACCCTGAT ATGAAGATGC CACCAGCAAC TGTTACTGAT 
CAGATCCAAG AAAGTCTCAA GAGGAAATCT TCTCTTACTG ATGAAGAGGC CACCAGTAGC 
TGGGAGAAAA GACCACGTAT CACTGAGAAT CGCCAGCACC AGCAGCCATT TCAGGTCTCC 
CCACAGCCTT TTCTTAATAG AGGGGACAGG GTCCAAGTGC GAAAAGTCCC ACCTCTCAAG 
ATCCCTGTCT CCAGAATCTC CCCGATGCTG TTTTCTACAT CACAGGTCTC TCCCAGGGCT 
CGTTTTCCAA TCTCCATCAC TAGTCCTTAC AGAACAGGAG CCAGAACTCT GGCAGACATC 
AAAGCAAAAG CCCAGTTGGT CAAAGCACAA AAGGCAGCAG CCGCAGCAGC TGCTGCAGCT 
GCTGCAGCCG CCTCAGTTGG AGGGACCATT CCGGGACCTG GCCCTGGGGG TGGACAAAGT 
CCAAGAGAGG GTGGTGAAAG GAAAATTGCT GGAGGAGGAA GTGCAGGCTC AGACCCAGTC 
AGTACAAATG GAAAAGGTCC CACACTGGAA CTGGCCGGAA CTGGAAGCAG GGGAGGTACG 
AGAGAACTTT TACCCTGTGG TCCTCAACCT GAGACCAATA TGCCAGGCCA AGCACAGCCT 
CCTGGTATCT CTGGAGCACA ACTACAGCAA ACCTCCTCAG TGCCTACGGG ACTTGCCAGC 
AGTGGAGCAT GCACAAGTGT CCCATTACCA GCCCACATAG AAATATCAAA CAGTGAAAAA 
CCAAACCTCC ACAAGGCAAC AGCCACAGCA GCCTCTCCTT GCCACTTGCA AGACCCTAGG 
AGTTGCAGAC TTGAAAAAGC TCTGTCTCCA ACAGGGCCTC CTCTGATCTC AGGAGCCTCA 
ACTGTTTATT TTGTAGCTGA TGGCACAGTT GAGCCCAAAG CAGGTTCTAA TAAGAATGCA 
CCAAAGCCTT CAGCCTTAGC AAAGACAACT GCTCCTGCTC CACTAGATAT GACTTCCTCT 
CCTGTGACAA CAGCCAGTTT AGAAAAACTC CCTGTACCCC AGATCAGTGG AACTGCAACA 
TCTACTGGAT CAGCTCCATC CTCAAGCACT TTGCCAGCAG CTTCTAGCCT TAAAACCCCA 
GGAACTTCTG CAAATATGAA TGGACCCATT TCAAGAACAA GTTCTAGTAT CCCTGCTAAT 
AATCCTTTAG TTACTCAGCT GCTCCAGGGC AAAGATGTTC CCTTGGAGCA AATTCTGCCT 
AAGCCTCTCA CCAAAATTGA AATGAAAACA GTTCCACTGA CTACAAAAGA GGAAAAAGGC 
ATAGGGATAT TCCCTGGTAT CAGTGTAATG GAAAGTAGCA GCAGAGAGGA AGTTAATGGC 
AGGCAGGCCC ATCTGGCTAT CCCGCAGTTG GGGAAACCCT TGCAAAGTAA ACAGCTTTCC 
CAGGTTCCAA GGCCAGTTTT CACTGCTAAG GACCGGAAGG ACCCCTGCAT TGACACTCAC 
CAGTACCGAG AAGGTCTAAG TAAAACAACG CAAGATCAGC TCTTTCAGAC TCTTATCCAG 
AGGGCTCAGA GACAGAGTGT TCTTTCATTT GTACCGCCCT CTCAGTTCAA CTTTGCTCAC 
TCAGGTTTCC ACTTGGAAGA CATCTCCACA AGTCAGAAGT TCATGCTGGG TTTTGCTGGC 
AGAAGGACAT CCAAGCCTGC AATGGCAGGT CACTATTTAC TTAATATTTC CACCTATGGC 
AGGGGTACAG AGAACATTAA AAGGACCCAT TCTGTAAATC CTGATGATCG ATTTTGTCTA 
AGTAGCCCCA CTGAGGCGTT GAGAATGGGG CATGCAGATT ATAAAAACAC AACTGGAGAA 
ATCAGCAGCA AAGAAGATGA AAGTGATGAA GATCGCGTAG GTGATGAACA GGAGCCTATT 
TCAGTGAAGG AGGAGCCATG GGCTTCCCAG AGTTCTGGCA GGCATCCGCA CCATGGGGAA 
GCTTCATCTA CCAATGATTG TTTAGCTAGC AAGAATGGTA AGACTGAGGC ACCAGTGAGT 
GAGCAAACCA CTTTAGGCCA GGAGAATTAC ATATTCTCTA GAGGCCAAGC ATCTGATGAA 
AAGAGCCTGC CTAGAGATTT TATTCCGGCA GCACATAAAC AGATGACTCA TGCTGTGAGA 
GGTAAGACAG TGTGTAGCAG CCCTGAGCTT TTCAATTCTA CTGCTCTTTC TCTTCCCGCA 
GACAGTCCCA CACATCAGCC TCTACTCCTT CCACCACTGC AAACCCCCAA ATTGTATGGA 
AGCCCCACAC AGATAGGGCC AAGCTATAGA GGCATGATCA ATGTCTCCAC CTCATCAGAC 
ATGGACCATA ACTCTGCTAT ACCAGGCAGC CAGGTATCTA GCAATGTAGG GGACGTCATG 
TCGTTTTCAG TGACTGTCAC TACCATCCCT GCCAGTCAAG CAATGAATCC CAGCAGCCAT 
GGCCAGACAA TTCCTGTTCA GACTTTTCCT GATGACAATA GTATCGAGGA CACACCATCA 
AAATGTTACT GCCGGTTGAA AGCCATGATC ATGTGCAAAG GATGTGGAGC TTTCTGCCAT 
GATGATTGCA TTGGCCCCTC CAAACTCTGT GTCTCCTGCC TTGTTGTTCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515302.1
CDS	240..4493
Translation	MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEISGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_006515239.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515239.4

ORF Insert Sequence:

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TGGGACTTCC CCTCTTGCAT GCTTGAATGC AATGCTGCAC 
ACAAACTCCC GAGGAGAAGA AGGCATCTTC TATAAAGTTC CAGGTAGAAT GGGCGTGTAT 
ACTTTGAAGA AAGATGTGCC AGATGGAGTG AAAGAGCTGT CAGAAGGTTC AGAAGAAAGC 
AGTGATGGTC AGTCAGATTC TCAGAGCTCT GAGAACAGCA GCAGCAGTGA TGGTGGCAGC 
AACAAAGAAG GGAGAAAGAG CAGGTGGAAA AGGAAAGTAT CATCTAGACT GTCACATCCA 
CCCTCTCCCC CGTCAGGCTG CCCATCACCC ACCATTCCAG CAAGTAAAGT CATTTCTCCA 
TCACAGAAGC ACAGCAAAAA GGCATTAAAA CAGGCCTTGA AGCAGCAACA ACAGAAGAAG 
AAGCAGCAGC AATGCAGACC AAGCATGTCC ATCTCCAATC AACACCTCTC TCTCAAGACT 
GTCAAAGCAG CCAGTGACTC TGTACCTGCC AAACCTGCCC TATGGGAAGG AAAGCAATCT 
GATGGACAGT CAAGCAGTCC CCAGAACTCA AATTCCAGCT TTTCTTCCTC AGTGAAAGTG 
GAAAGTTCTC TGCTAGGCTT GGGGAAGAAG TCTTTCCAGA GGTCTGACAG ACTCCACACA 
AGACAGATGA AAAGGACTAA ATGTGCTGAC ATTGATGTTG AGACTCCTGA TTCCATTCTG 
GTTAATACTA ATCTTCGAGC ACTGATCAAC AAGCACACCT TTTCAGTTCT TCCTGGAGAC 
TGCCAGCAGC GCCTGCTTTT GTTGCTTCCA GAGGTGGATC GACAGGTTGG TCCAGATGGC 
CTGATGAAGT TGAATGGCTC AGCCCTTAAC AATGAGTTCT TCACTTCTGC AGCCCAGGGC 
TGGAAGGAAA GGCTCTCAGA AGGTGAATTT ACTCCTGAAA TGCAGGTGAG AATTCGACAA 
GAGATTGAGA AGGAGAAGAA AGTAGAGCTG TGGAAGGAAC AATTCTTTGA AAATTACTAT 
GGTCAGAGTT CTGGCCTAAG CCTTGAAGAT TCACAGAAAC TGACAGCTTC CTCAAGTGAT 
CCCAAAGCAA AGAAAACCCC AGCTGAGCAA CCAAAATCCA TACTTCCTTC AGAGGCCTCT 
CCTGTCAGAA TTGTCCCAGT AGTTCCTCAG TCCGAGTGTA AAGAAGAAGC AGTGCAGATA 
CCATCACCAT CCCAAAAGGA AGAAAATCAA GACGAGGCGA GGCCAGACTC CAAATCCCCA 
GAACCTGTTC TTGCATCAGC TAGCAATACA AATGAGCTTA TCACCATGAA ACCCATCAAG 
AGCCCTAAGG ATGAGGGTCT CTTAGAGCAG AAGCCTGTTG CCTGTGCTGA ACAAGAATCT 
GAGAAAGAAA ATCATGTAAC CACAACTTCT CGTAATAACA AAAGTGAGAA CCAAGAAGCG 
TTAGCTATAT CCCCAAGCAA ATCTAAAAAT GCTGGGTTAC AAAAACCCAT AATAAAGCCT 
GTTGCAGAAG CCAGTCCACT GAACCCTGAT ATGAAGATGC CACCAGCAAC TGTTACTGAT 
CAGATCCAAG AAAGTCTCAA GAGGAAATCT TCTCTTACTG ATGAAGAGGC CACCAGTAGC 
TGGGAGAAAA GACCACGTAT CACTGAGAAT CGCCAGCACC AGCAGCCATT TCAGGTCTCC 
CCACAGCCTT TTCTTAATAG AGGGGACAGG GTCCAAGTGC GAAAAGTCCC ACCTCTCAAG 
ATCCCTGTCT CCAGAATCTC CCCGATGCTG TTTTCTACAT CACAGGTCTC TCCCAGGGCT 
CGTTTTCCAA TCTCCATCAC TAGTCCTTAC AGAACAGGAG CCAGAACTCT GGCAGACATC 
AAAGCAAAAG CCCAGTTGGT CAAAGCACAA AAGGCAGCAG CCGCAGCAGC TGCTGCAGCT 
GCTGCAGCCG CCTCAGTTGG AGGGACCATT CCGGGACCTG GCCCTGGGGG TGGACAAAGT 
CCAAGAGAGG GTGGTGAAAG GAAAATTGCT GGAGGAGGAA GTGCAGGCTC AGACCCAGTC 
AGTACAAATG GAAAAGGTCC CACACTGGAA CTGGCCGGAA CTGGAAGCAG GGGAGGTACG 
AGAGAACTTT TACCCTGTGG TCCTCAACCT GAGACCAATA TGCCAGGCCA AGCACAGCCT 
CCTGGTATCT CTGGAGCACA ACTACAGCAA ACCTCCTCAG TGCCTACGGG ACTTGCCAGC 
AGTGGAGCAT GCACAAGTGT CCCATTACCA GCCCACATAG AAATATCAAA CAGTGAAAAA 
CCAAACCTCC ACAAGGCAAC AGCCACAGCA GCCTCTCCTT GCCACTTGCA AGACCCTAGG 
AGTTGCAGAC TTGAAAAAGC TCTGTCTCCA ACAGGGCCTC CTCTGATCTC AGGAGCCTCA 
ACTGTTTATT TTGTAGCTGA TGGCACAGTT GAGCCCAAAG CAGGTTCTAA TAAGAATGCA 
CCAAAGCCTT CAGCCTTAGC AAAGACAACT GCTCCTGCTC CACTAGATAT GACTTCCTCT 
CCTGTGACAA CAGCCAGTTT AGAAAAACTC CCTGTACCCC AGATCAGTGG AACTGCAACA 
TCTACTGGAT CAGCTCCATC CTCAAGCACT TTGCCAGCAG CTTCTAGCCT TAAAACCCCA 
GGAACTTCTG CAAATATGAA TGGACCCATT TCAAGAACAA GTTCTAGTAT CCCTGCTAAT 
AATCCTTTAG TTACTCAGCT GCTCCAGGGC AAAGATGTTC CCTTGGAGCA AATTCTGCCT 
AAGCCTCTCA CCAAAATTGA AATGAAAACA GTTCCACTGA CTACAAAAGA GGAAAAAGGC 
ATAGGGATAT TCCCTGGTAT CAGTGTAATG GAAAGTAGCA GCAGAGAGGA AGTTAATGGC 
AGGCAGGCCC ATCTGGCTAT CCCGCAGTTG GGGAAACCCT TGCAAAGTAA ACAGCTTTCC 
CAGGTTCCAA GGCCAGTTTT CACTGCTAAG GACCGGAAGG ACCCCTGCAT TGACACTCAC 
CAGTACCGAG AAGGTCTAAG TAAAACAACG CAAGATCAGC TCTTTCAGAC TCTTATCCAG 
AGGGCTCAGA GACAGAGTGT TCTTTCATTT GTACCGCCCT CTCAGTTCAA CTTTGCTCAC 
TCAGGTTTCC ACTTGGAAGA CATCTCCACA AGTCAGAAGT TCATGCTGGG TTTTGCTGGC 
AGAAGGACAT CCAAGCCTGC AATGGCAGGT CACTATTTAC TTAATATTTC CACCTATGGC 
AGGGGTACAG AGAACATTAA AAGGACCCAT TCTGTAAATC CTGATGATCG ATTTTGTCTA 
AGTAGCCCCA CTGAGGCGTT GAGAATGGGG CATGCAGATT ATAAAAACAC AACTGGAGAA 
ATCAGCAGCA AAGAAGATGA AAGTGATGAA GATCGCGTAG GTGATGAACA GGAGCCTATT 
TCAGTGAAGG AGGAGCCATG GGCTTCCCAG AGTTCTGGCA GGCATCCGCA CCATGGGGAA 
GCTTCATCTA CCAATGATTG TTTAGCTAGC AAGAATGGTA AGACTGAGGC ACCAGTGAGT 
GAGCAAACCA CTTTAGGCCA GGAGAATTAC ATATTCTCTA GAGGCCAAGC ATCTGATGAA 
AAGAGCCTGC CTAGAGATTT TATTCCGGCA GCACATAAAC AGATGACTCA TGCTGTGAGA 
GGTAAGACAG TGTGTAGCAG CCCTGAGCTT TTCAATTCTA CTGCTCTTTC TCTTCCCGCA 
GACAGTCCCA CACATCAGCC TCTACTCCTT CCACCACTGC AAACCCCCAA ATTGTATGGA 
AGCCCCACAC AGATAGGGCC AAGCTATAGA GGCATGATCA ATGTCTCCAC CTCATCAGAC 
ATGGACCATA ACTCTGCTAT ACCAGGCAGC CAGGTATCTA GCAATGTAGG GGACGTCATG 
TCGTTTTCAG TGACTGTCAC TACCATCCCT GCCAGTCAAG CAATGAATCC CAGCAGCCAT 
GGCCAGACAA TTCCTGTTCA GACTTTTCCT GATGACAATA GTATCGAGGA CACACCATCA 
AAATGTTACT GCCGGTTGAA AGCCATGATC ATGTGCAAAG GATGTGGAGC TTTCTGCCAT 
GATGATTGCA TTGGCCCCTC CAAACTCTGT GTCTCCTGCC TTGTTGTTCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_030246972.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102832.1 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu47432
Clone ID Related Accession (Same CDS sequence)	XM_030246972.1 , XM_006515242.2
Accession Version	XM_030246972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAAA AGAAAGGACA GATGAAAAGG ACTAAATGTG CTGACATTGA TGTTGAGACT 
CCTGATTCCA TTCTGGTTAA TACTAATCTT CGAGCACTGA TCAACAAGCA CACCTTTTCA 
GTTCTTCCTG GAGACTGCCA GCAGCGCCTG CTTTTGTTGC TTCCAGAGGT GGATCGACAG 
GTTGGTCCAG ATGGCCTGAT GAAGTTGAAT GGCTCAGCCC TTAACAATGA GTTCTTCACT 
TCTGCAGCCC AGGGCTGGAA GGAAAGGCTC TCAGAAGGTG AATTTACTCC TGAAATGCAG 
GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCTGTGGAA GGAACAATTC 
TTTGAAAATT ACTATGGTCA GAGTTCTGGC CTAAGCCTTG AAGATTCACA GAAACTGACA 
GCTTCCTCAA GTGATCCCAA AGCAAAGAAA ACCCCAGCTG AGCAACCAAA ATCCATACTT 
CCTTCAGAGG CCTCTCCTGT CAGAATTGTC CCAGTAGTTC CTCAGTCCGA GTGTAAAGAA 
GAAGCAGTGC AGATACCATC ACCATCCCAA AAGGAAGAAA ATCAAGACGA GGCGAGGCCA 
GACTCCAAAT CCCCAGAACC TGTTCTTGCA TCAGCTAGCA ATACAAATGA GCTTATCACC 
ATGAAACCCA TCAAGAGCCC TAAGGATGAG GGTCTCTTAG AGCAGAAGCC TGTTGCCTGT 
GCTGAACAAG AATCTGAGAA AGAAAATCAT GTAACCACAA CTTCTCGTAA TAACAAAAGT 
GAGAACCAAG AAGCGTTAGC TATATCCCCA AGCAAATCTA AAAATGCTGG GTTACAAAAA 
CCCATAATAA AGCCTGTTGC AGAAGCCAGT CCACTGAACC CTGATATGAA GATGCCACCA 
GCAACTGTTA CTGATCAGAT CCAAGAAAGT CTCAAGAGGA AATCTTCTCT TACTGATGAA 
GAGGCCACCA GTAGCTGGGA GAAAAGACCA CGTATCACTG AGAATCGCCA GCACCAGCAG 
CCATTTCAGG TCTCCCCACA GCCTTTTCTT AATAGAGGGG ACAGGGTCCA AGTGCGAAAA 
GTCCCACCTC TCAAGATCCC TGTCTCCAGA ATCTCCCCGA TGCTGTTTTC TACATCACAG 
GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTTACAGAAC AGGAGCCAGA 
ACTCTGGCAG ACATCAAAGC AAAAGCCCAG TTGGTCAAAG CACAAAAGGC AGCAGCCGCA 
GCAGCTGCTG CAGCTGCTGC AGCCGCCTCA GTTGGAGGGA CCATTCCGGG ACCTGGCCCT 
GGGGGTGGAC AAAGTCCAAG AGAGGGTGGT GAAAGGAAAA TTGCTGGAGG AGGAAGTGCA 
GGCTCAGACC CAGTCAGTAC AAATGGAAAA GGTCCCACAC TGGAACTGGC CGGAACTGGA 
AGCAGGGGAG GTACGAGAGA ACTTTTACCC TGTGGTCCTC AACCTGAGAC CAATATGCCA 
GGCCAAGCAC AGCCTCCTGG TATCTCTGGA GCACAACTAC AGCAAACCTC CTCAGTGCCT 
ACGGGACTTG CCAGCAGTGG AGCATGCACA AGTGTCCCAT TACCAGCCCA CATAGAAATA 
TCAAACAGTG AAAAACCAAA CCTCCACAAG GCAACAGCCA CAGCAGCCTC TCCTTGCCAC 
TTGCAAGACC CTAGGAGTTG CAGACTTGAA AAAGCTCTGT CTCCAACAGG GCCTCCTCTG 
ATCTCAGGAG CCTCAACTGT TTATTTTGTA GCTGATGGCA CAGTTGAGCC CAAAGCAGGT 
TCTAATAAGA ATGCACCAAA GCCTTCAGCC TTAGCAAAGA CAACTGCTCC TGCTCCACTA 
GATATGACTT CCTCTCCTGT GACAACAGCC AGTTTAGAAA AACTCCCTGT ACCCCAGATC 
AGTGGAACTG CAACATCTAC TGGATCAGCT CCATCCTCAA GCACTTTGCC AGCAGCTTCT 
AGCCTTAAAA CCCCAGGAAC TTCTGCAAAT ATGAATGGAC CCATTTCAAG AACAAGTTCT 
AGTATCCCTG CTAATAATCC TTTAGTTACT CAGCTGCTCC AGGGCAAAGA TGTTCCCTTG 
GAGCAAATTC TGCCTAAGCC TCTCACCAAA ATTGAAATGA AAACAGTTCC ACTGACTACA 
AAAGAGGAAA AAGGCATAGG GATATTCCCT GGTATCAGTG TAATGGAAAG TAGCAGCAGA 
GAGGAAGTTA ATGGCAGGCA GGCCCATCTG GCTATCCCGC AGTTGGGGAA ACCCTTGCAA 
AGTAAACAGC TTTCCCAGGT TCCAAGGCCA GTTTTCACTG CTAAGGACCG GAAGGACCCC 
TGCATTGACA CTCACCAGTA CCGAGAAGGT CTAAGTAAAA CAACGCAAGA TCAGCTCTTT 
CAGACTCTTA TCCAGAGGGC TCAGAGACAG AGTGTTCTTT CATTTGTACC GCCCTCTCAG 
TTCAACTTTG CTCACTCAGG TTTCCACTTG GAAGACATCT CCACAAGTCA GAAGTTCATG 
CTGGGTTTTG CTGGCAGAAG GACATCCAAG CCTGCAATGG CAGGTCACTA TTTACTTAAT 
ATTTCCACCT ATGGCAGGGG TACAGAGAAC ATTAAAAGGA CCCATTCTGT AAATCCTGAT 
GATCGATTTT GTCTAAGTAG CCCCACTGAG GCGTTGAGAA TGGGGCATGC AGATTATAAA 
AACACAACTG GAGAAATCAG CAGCAAAGAA GATGAAAGTG ATGAAGATCG CGTAGGTGAT 
GAACAGGAGC CTATTTCAGT GAAGGAGGAG CCATGGGCTT CCCAGAGTTC TGGCAGGCAT 
CCGCACCATG GGGAAGCTTC ATCTACCAAT GATTGTTTAG CTAGCAAGAA TGGTAAGACT 
GAGGCACCAG TGAGTGAGCA AACCACTTTA GGCCAGGAGA ATTACATATT CTCTAGAGGC 
CAAGCATCTG ATGAAAAGAG CCTGCCTAGA GATTTTATTC CGGCAGCACA TAAACAGATG 
ACTCATGCTG TGAGAGGTAA GACAGTGTGT AGCAGCCCTG AGCTTTTCAA TTCTACTGCT 
CTTTCTCTTC CCGCAGACAG TCCCACACAT CAGCCTCTAC TCCTTCCACC ACTGCAAACC 
CCCAAATTGT ATGGAAGCCC CACACAGATA GGGCCAAGCT ATAGAGGCAT GATCAATGTC 
TCCACCTCAT CAGACATGGA CCATAACTCT GCTATACCAG GCAGCCAGGT ATCTAGCAAT 
GTAGGGGACG TCATGTCGTT TTCAGTGACT GTCACTACCA TCCCTGCCAG TCAAGCAATG 
AATCCCAGCA GCCATGGCCA GACAATTCCT GTTCAGACTT TTCCTGATGA CAATAGTATC 
GAGGACACAC CATCAAAATG TTACTGCCGG TTGAAAGCCA TGATCATGTG CAAAGGATGT 
GGAGCTTTCT GCCATGATGA TTGCATTGGC CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT 
GTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102832.1
CDS	980..4468
Translation	MTEKKGQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_030246972.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246972.1

ORF Insert Sequence:

ATGACAGAAA AGAAAGGACA GATGAAAAGG ACTAAATGTG CTGACATTGA TGTTGAGACT 
CCTGATTCCA TTCTGGTTAA TACTAATCTT CGAGCACTGA TCAACAAGCA CACCTTTTCA 
GTTCTTCCTG GAGACTGCCA GCAGCGCCTG CTTTTGTTGC TTCCAGAGGT GGATCGACAG 
GTTGGTCCAG ATGGCCTGAT GAAGTTGAAT GGCTCAGCCC TTAACAATGA GTTCTTCACT 
TCTGCAGCCC AGGGCTGGAA GGAAAGGCTC TCAGAAGGTG AATTTACTCC TGAAATGCAG 
GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCTGTGGAA GGAACAATTC 
TTTGAAAATT ACTATGGTCA GAGTTCTGGC CTAAGCCTTG AAGATTCACA GAAACTGACA 
GCTTCCTCAA GTGATCCCAA AGCAAAGAAA ACCCCAGCTG AGCAACCAAA ATCCATACTT 
CCTTCAGAGG CCTCTCCTGT CAGAATTGTC CCAGTAGTTC CTCAGTCCGA GTGTAAAGAA 
GAAGCAGTGC AGATACCATC ACCATCCCAA AAGGAAGAAA ATCAAGACGA GGCGAGGCCA 
GACTCCAAAT CCCCAGAACC TGTTCTTGCA TCAGCTAGCA ATACAAATGA GCTTATCACC 
ATGAAACCCA TCAAGAGCCC TAAGGATGAG GGTCTCTTAG AGCAGAAGCC TGTTGCCTGT 
GCTGAACAAG AATCTGAGAA AGAAAATCAT GTAACCACAA CTTCTCGTAA TAACAAAAGT 
GAGAACCAAG AAGCGTTAGC TATATCCCCA AGCAAATCTA AAAATGCTGG GTTACAAAAA 
CCCATAATAA AGCCTGTTGC AGAAGCCAGT CCACTGAACC CTGATATGAA GATGCCACCA 
GCAACTGTTA CTGATCAGAT CCAAGAAAGT CTCAAGAGGA AATCTTCTCT TACTGATGAA 
GAGGCCACCA GTAGCTGGGA GAAAAGACCA CGTATCACTG AGAATCGCCA GCACCAGCAG 
CCATTTCAGG TCTCCCCACA GCCTTTTCTT AATAGAGGGG ACAGGGTCCA AGTGCGAAAA 
GTCCCACCTC TCAAGATCCC TGTCTCCAGA ATCTCCCCGA TGCTGTTTTC TACATCACAG 
GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTTACAGAAC AGGAGCCAGA 
ACTCTGGCAG ACATCAAAGC AAAAGCCCAG TTGGTCAAAG CACAAAAGGC AGCAGCCGCA 
GCAGCTGCTG CAGCTGCTGC AGCCGCCTCA GTTGGAGGGA CCATTCCGGG ACCTGGCCCT 
GGGGGTGGAC AAAGTCCAAG AGAGGGTGGT GAAAGGAAAA TTGCTGGAGG AGGAAGTGCA 
GGCTCAGACC CAGTCAGTAC AAATGGAAAA GGTCCCACAC TGGAACTGGC CGGAACTGGA 
AGCAGGGGAG GTACGAGAGA ACTTTTACCC TGTGGTCCTC AACCTGAGAC CAATATGCCA 
GGCCAAGCAC AGCCTCCTGG TATCTCTGGA GCACAACTAC AGCAAACCTC CTCAGTGCCT 
ACGGGACTTG CCAGCAGTGG AGCATGCACA AGTGTCCCAT TACCAGCCCA CATAGAAATA 
TCAAACAGTG AAAAACCAAA CCTCCACAAG GCAACAGCCA CAGCAGCCTC TCCTTGCCAC 
TTGCAAGACC CTAGGAGTTG CAGACTTGAA AAAGCTCTGT CTCCAACAGG GCCTCCTCTG 
ATCTCAGGAG CCTCAACTGT TTATTTTGTA GCTGATGGCA CAGTTGAGCC CAAAGCAGGT 
TCTAATAAGA ATGCACCAAA GCCTTCAGCC TTAGCAAAGA CAACTGCTCC TGCTCCACTA 
GATATGACTT CCTCTCCTGT GACAACAGCC AGTTTAGAAA AACTCCCTGT ACCCCAGATC 
AGTGGAACTG CAACATCTAC TGGATCAGCT CCATCCTCAA GCACTTTGCC AGCAGCTTCT 
AGCCTTAAAA CCCCAGGAAC TTCTGCAAAT ATGAATGGAC CCATTTCAAG AACAAGTTCT 
AGTATCCCTG CTAATAATCC TTTAGTTACT CAGCTGCTCC AGGGCAAAGA TGTTCCCTTG 
GAGCAAATTC TGCCTAAGCC TCTCACCAAA ATTGAAATGA AAACAGTTCC ACTGACTACA 
AAAGAGGAAA AAGGCATAGG GATATTCCCT GGTATCAGTG TAATGGAAAG TAGCAGCAGA 
GAGGAAGTTA ATGGCAGGCA GGCCCATCTG GCTATCCCGC AGTTGGGGAA ACCCTTGCAA 
AGTAAACAGC TTTCCCAGGT TCCAAGGCCA GTTTTCACTG CTAAGGACCG GAAGGACCCC 
TGCATTGACA CTCACCAGTA CCGAGAAGGT CTAAGTAAAA CAACGCAAGA TCAGCTCTTT 
CAGACTCTTA TCCAGAGGGC TCAGAGACAG AGTGTTCTTT CATTTGTACC GCCCTCTCAG 
TTCAACTTTG CTCACTCAGG TTTCCACTTG GAAGACATCT CCACAAGTCA GAAGTTCATG 
CTGGGTTTTG CTGGCAGAAG GACATCCAAG CCTGCAATGG CAGGTCACTA TTTACTTAAT 
ATTTCCACCT ATGGCAGGGG TACAGAGAAC ATTAAAAGGA CCCATTCTGT AAATCCTGAT 
GATCGATTTT GTCTAAGTAG CCCCACTGAG GCGTTGAGAA TGGGGCATGC AGATTATAAA 
AACACAACTG GAGAAATCAG CAGCAAAGAA GATGAAAGTG ATGAAGATCG CGTAGGTGAT 
GAACAGGAGC CTATTTCAGT GAAGGAGGAG CCATGGGCTT CCCAGAGTTC TGGCAGGCAT 
CCGCACCATG GGGAAGCTTC ATCTACCAAT GATTGTTTAG CTAGCAAGAA TGGTAAGACT 
GAGGCACCAG TGAGTGAGCA AACCACTTTA GGCCAGGAGA ATTACATATT CTCTAGAGGC 
CAAGCATCTG ATGAAAAGAG CCTGCCTAGA GATTTTATTC CGGCAGCACA TAAACAGATG 
ACTCATGCTG TGAGAGGTAA GACAGTGTGT AGCAGCCCTG AGCTTTTCAA TTCTACTGCT 
CTTTCTCTTC CCGCAGACAG TCCCACACAT CAGCCTCTAC TCCTTCCACC ACTGCAAACC 
CCCAAATTGT ATGGAAGCCC CACACAGATA GGGCCAAGCT ATAGAGGCAT GATCAATGTC 
TCCACCTCAT CAGACATGGA CCATAACTCT GCTATACCAG GCAGCCAGGT ATCTAGCAAT 
GTAGGGGACG TCATGTCGTT TTCAGTGACT GTCACTACCA TCCCTGCCAG TCAAGCAATG 
AATCCCAGCA GCCATGGCCA GACAATTCCT GTTCAGACTT TTCCTGATGA CAATAGTATC 
GAGGACACAC CATCAAAATG TTACTGCCGG TTGAAAGCCA TGATCATGTG CAAAGGATGT 
GGAGCTTTCT GCCATGATGA TTGCATTGGC CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT 
GTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_030246971.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102831.1 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X6, mRNA.

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CloneID	OMu71297
Clone ID Related Accession (Same CDS sequence)	XM_030246971.1 , XM_030246970.1
Accession Version	XM_030246971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCT CCAATCAACA CCTCTCTCTC AAGACTGTCA AAGCAGCCAG TGACTCTGTA 
CCTGCCAAAC CTGCCCTATG GGAAGGAAAG CAATCTGATG GACAGTCAAG CAGTCCCCAG 
AACTCAAATT CCAGCTTTTC TTCCTCAGTG AAAGTGGAAA GTTCTCTGCT AGGCTTGGGG 
AAGAAGTCTT TCCAGAGGTC TGACAGACTC CACACAAGAC AGATGAAAAG GACTAAATGT 
GCTGACATTG ATGTTGAGAC TCCTGATTCC ATTCTGGTTA ATACTAATCT TCGAGCACTG 
ATCAACAAGC ACACCTTTTC AGTTCTTCCT GGAGACTGCC AGCAGCGCCT GCTTTTGTTG 
CTTCCAGAGG TGGATCGACA GGTTGGTCCA GATGGCCTGA TGAAGTTGAA TGGCTCAGCC 
CTTAACAATG AGTTCTTCAC TTCTGCAGCC CAGGGCTGGA AGGAAAGGCT CTCAGAAGGT 
GAATTTACTC CTGAAATGCA GGTGAGAATT CGACAAGAGA TTGAGAAGGA GAAGAAAGTA 
GAGCTGTGGA AGGAACAATT CTTTGAAAAT TACTATGGTC AGAGTTCTGG CCTAAGCCTT 
GAAGATTCAC AGAAACTGAC AGCTTCCTCA AGTGATCCCA AAGCAAAGAA AACCCCAGCT 
GAGCAACCAA AATCCATACT TCCTTCAGAG GCCTCTCCTG TCAGAATTGT CCCAGTAGTT 
CCTCAGTCCG AGTGTAAAGA AGAAGCAGTG CAGATACCAT CACCATCCCA AAAGGAAGAA 
AATCAAGACG AGGCGAGGCC AGACTCCAAA TCCCCAGAAC CTGTTCTTGC ATCAGCTAGC 
AATACAAATG AGCTTATCAC CATGAAACCC ATCAAGAGCC CTAAGGATGA GGGTCTCTTA 
GAGCAGAAGC CTGTTGCCTG TGCTGAACAA GAATCTGAGA AAGAAAATCA TGTAACCACA 
ACTTCTCGTA ATAACAAAAG TGAGAACCAA GAAGCGTTAG CTATATCCCC AAGCAAATCT 
AAAAATGCTG GGTTACAAAA ACCCATAATA AAGCCTGTTG CAGAAGCCAG TCCACTGAAC 
CCTGATATGA AGATGCCACC AGCAACTGTT ACTGATCAGA TCCAAGAAAG TCTCAAGAGG 
AAATCTTCTC TTACTGATGA AGAGGCCACC AGTAGCTGGG AGAAAAGACC ACGTATCACT 
GAGAATCGCC AGCACCAGCA GCCATTTCAG GTCTCCCCAC AGCCTTTTCT TAATAGAGGG 
GACAGGGTCC AAGTGCGAAA AGTCCCACCT CTCAAGATCC CTGTCTCCAG AATCTCCCCG 
ATGCTGTTTT CTACATCACA GGTCTCTCCC AGGGCTCGTT TTCCAATCTC CATCACTAGT 
CCTTACAGAA CAGGAGCCAG AACTCTGGCA GACATCAAAG CAAAAGCCCA GTTGGTCAAA 
GCACAAAAGG CAGCAGCCGC AGCAGCTGCT GCAGCTGCTG CAGCCGCCTC AGTTGGAGGG 
ACCATTCCGG GACCTGGCCC TGGGGGTGGA CAAAGTCCAA GAGAGGGTGG TGAAAGGAAA 
ATTGCTGGAG GAGGAAGTGC AGGCTCAGAC CCAGTCAGTA CAAATGGAAA AGGTCCCACA 
CTGGAACTGG CCGGAACTGG AAGCAGGGGA GGTACGAGAG AACTTTTACC CTGTGGTCCT 
CAACCTGAGA CCAATATGCC AGGCCAAGCA CAGCCTCCTG GTATCTCTGG AGCACAACTA 
CAGCAAACCT CCTCAGTGCC TACGGGACTT GCCAGCAGTG GAGCATGCAC AAGTGTCCCA 
TTACCAGCCC ACATAGAAAT ATCAAACAGT GAAAAACCAA ACCTCCACAA GGCAACAGCC 
ACAGCAGCCT CTCCTTGCCA CTTGCAAGAC CCTAGGAGTT GCAGACTTGA AAAAGCTCTG 
TCTCCAACAG GGCCTCCTCT GATCTCAGGA GCCTCAACTG TTTATTTTGT AGCTGATGGC 
ACAGTTGAGC CCAAAGCAGG TTCTAATAAG AATGCACCAA AGCCTTCAGC CTTAGCAAAG 
ACAACTGCTC CTGCTCCACT AGATATGACT TCCTCTCCTG TGACAACAGC CAGTTTAGAA 
AAACTCCCTG TACCCCAGAT CAGTGGAACT GCAACATCTA CTGGATCAGC TCCATCCTCA 
AGCACTTTGC CAGCAGCTTC TAGCCTTAAA ACCCCAGGAA CTTCTGCAAA TATGAATGGA 
CCCATTTCAA GAACAAGTTC TAGTATCCCT GCTAATAATC CTTTAGTTAC TCAGCTGCTC 
CAGGGCAAAG ATGTTCCCTT GGAGCAAATT CTGCCTAAGC CTCTCACCAA AATTGAAATG 
AAAACAGTTC CACTGACTAC AAAAGAGGAA AAAGGCATAG GGATATTCCC TGGTATCAGT 
GTAATGGAAA GTAGCAGCAG AGAGGAAGTT AATGGCAGGC AGGCCCATCT GGCTATCCCG 
CAGTTGGGGA AACCCTTGCA AAGTAAACAG CTTTCCCAGG TTCCAAGGCC AGTTTTCACT 
GCTAAGGACC GGAAGGACCC CTGCATTGAC ACTCACCAGT ACCGAGAAGG TCTAAGTAAA 
ACAACGCAAG ATCAGCTCTT TCAGACTCTT ATCCAGAGGG CTCAGAGACA GAGTGTTCTT 
TCATTTGTAC CGCCCTCTCA GTTCAACTTT GCTCACTCAG GTTTCCACTT GGAAGACATC 
TCCACAAGTC AGAAGTTCAT GCTGGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCAATG 
GCAGGTCACT ATTTACTTAA TATTTCCACC TATGGCAGGG GTACAGAGAA CATTAAAAGG 
ACCCATTCTG TAAATCCTGA TGATCGATTT TGTCTAAGTA GCCCCACTGA GGCGTTGAGA 
ATGGGGCATG CAGATTATAA AAACACAACT GGAGAAATCA GCAGCAAAGA AGATGAAAGT 
GATGAAGATC GCGTAGGTGA TGAACAGGAG CCTATTTCAG TGAAGGAGGA GCCATGGGCT 
TCCCAGAGTT CTGGCAGGCA TCCGCACCAT GGGGAAGCTT CATCTACCAA TGATTGTTTA 
GCTAGCAAGA ATGGTAAGAC TGAGGCACCA GTGAGTGAGC AAACCACTTT AGGCCAGGAG 
AATTACATAT TCTCTAGAGG CCAAGCATCT GATGAAAAGA GCCTGCCTAG AGATTTTATT 
CCGGCAGCAC ATAAACAGAT GACTCATGCT GTGAGAGGTA AGACAGTGTG TAGCAGCCCT 
GAGCTTTTCA ATTCTACTGC TCTTTCTCTT CCCGCAGACA GTCCCACACA TCAGCCTCTA 
CTCCTTCCAC CACTGCAAAC CCCCAAATTG TATGGAAGCC CCACACAGAT AGGGCCAAGC 
TATAGAGGCA TGATCAATGT CTCCACCTCA TCAGACATGG ACCATAACTC TGCTATACCA 
GGCAGCCAGG TATCTAGCAA TGTAGGGGAC GTCATGTCGT TTTCAGTGAC TGTCACTACC 
ATCCCTGCCA GTCAAGCAAT GAATCCCAGC AGCCATGGCC AGACAATTCC TGTTCAGACT 
TTTCCTGATG ACAATAGTAT CGAGGACACA CCATCAAAAT GTTACTGCCG GTTGAAAGCC 
ATGATCATGT GCAAAGGATG TGGAGCTTTC TGCCATGATG ATTGCATTGG CCCCTCCAAA 
CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102831.1
CDS	258..3947
Translation	MSISNQHLSLKTVKAASDSVPAKPALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_030246971.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246971.1

ORF Insert Sequence:

ATGTCCATCT CCAATCAACA CCTCTCTCTC AAGACTGTCA AAGCAGCCAG TGACTCTGTA 
CCTGCCAAAC CTGCCCTATG GGAAGGAAAG CAATCTGATG GACAGTCAAG CAGTCCCCAG 
AACTCAAATT CCAGCTTTTC TTCCTCAGTG AAAGTGGAAA GTTCTCTGCT AGGCTTGGGG 
AAGAAGTCTT TCCAGAGGTC TGACAGACTC CACACAAGAC AGATGAAAAG GACTAAATGT 
GCTGACATTG ATGTTGAGAC TCCTGATTCC ATTCTGGTTA ATACTAATCT TCGAGCACTG 
ATCAACAAGC ACACCTTTTC AGTTCTTCCT GGAGACTGCC AGCAGCGCCT GCTTTTGTTG 
CTTCCAGAGG TGGATCGACA GGTTGGTCCA GATGGCCTGA TGAAGTTGAA TGGCTCAGCC 
CTTAACAATG AGTTCTTCAC TTCTGCAGCC CAGGGCTGGA AGGAAAGGCT CTCAGAAGGT 
GAATTTACTC CTGAAATGCA GGTGAGAATT CGACAAGAGA TTGAGAAGGA GAAGAAAGTA 
GAGCTGTGGA AGGAACAATT CTTTGAAAAT TACTATGGTC AGAGTTCTGG CCTAAGCCTT 
GAAGATTCAC AGAAACTGAC AGCTTCCTCA AGTGATCCCA AAGCAAAGAA AACCCCAGCT 
GAGCAACCAA AATCCATACT TCCTTCAGAG GCCTCTCCTG TCAGAATTGT CCCAGTAGTT 
CCTCAGTCCG AGTGTAAAGA AGAAGCAGTG CAGATACCAT CACCATCCCA AAAGGAAGAA 
AATCAAGACG AGGCGAGGCC AGACTCCAAA TCCCCAGAAC CTGTTCTTGC ATCAGCTAGC 
AATACAAATG AGCTTATCAC CATGAAACCC ATCAAGAGCC CTAAGGATGA GGGTCTCTTA 
GAGCAGAAGC CTGTTGCCTG TGCTGAACAA GAATCTGAGA AAGAAAATCA TGTAACCACA 
ACTTCTCGTA ATAACAAAAG TGAGAACCAA GAAGCGTTAG CTATATCCCC AAGCAAATCT 
AAAAATGCTG GGTTACAAAA ACCCATAATA AAGCCTGTTG CAGAAGCCAG TCCACTGAAC 
CCTGATATGA AGATGCCACC AGCAACTGTT ACTGATCAGA TCCAAGAAAG TCTCAAGAGG 
AAATCTTCTC TTACTGATGA AGAGGCCACC AGTAGCTGGG AGAAAAGACC ACGTATCACT 
GAGAATCGCC AGCACCAGCA GCCATTTCAG GTCTCCCCAC AGCCTTTTCT TAATAGAGGG 
GACAGGGTCC AAGTGCGAAA AGTCCCACCT CTCAAGATCC CTGTCTCCAG AATCTCCCCG 
ATGCTGTTTT CTACATCACA GGTCTCTCCC AGGGCTCGTT TTCCAATCTC CATCACTAGT 
CCTTACAGAA CAGGAGCCAG AACTCTGGCA GACATCAAAG CAAAAGCCCA GTTGGTCAAA 
GCACAAAAGG CAGCAGCCGC AGCAGCTGCT GCAGCTGCTG CAGCCGCCTC AGTTGGAGGG 
ACCATTCCGG GACCTGGCCC TGGGGGTGGA CAAAGTCCAA GAGAGGGTGG TGAAAGGAAA 
ATTGCTGGAG GAGGAAGTGC AGGCTCAGAC CCAGTCAGTA CAAATGGAAA AGGTCCCACA 
CTGGAACTGG CCGGAACTGG AAGCAGGGGA GGTACGAGAG AACTTTTACC CTGTGGTCCT 
CAACCTGAGA CCAATATGCC AGGCCAAGCA CAGCCTCCTG GTATCTCTGG AGCACAACTA 
CAGCAAACCT CCTCAGTGCC TACGGGACTT GCCAGCAGTG GAGCATGCAC AAGTGTCCCA 
TTACCAGCCC ACATAGAAAT ATCAAACAGT GAAAAACCAA ACCTCCACAA GGCAACAGCC 
ACAGCAGCCT CTCCTTGCCA CTTGCAAGAC CCTAGGAGTT GCAGACTTGA AAAAGCTCTG 
TCTCCAACAG GGCCTCCTCT GATCTCAGGA GCCTCAACTG TTTATTTTGT AGCTGATGGC 
ACAGTTGAGC CCAAAGCAGG TTCTAATAAG AATGCACCAA AGCCTTCAGC CTTAGCAAAG 
ACAACTGCTC CTGCTCCACT AGATATGACT TCCTCTCCTG TGACAACAGC CAGTTTAGAA 
AAACTCCCTG TACCCCAGAT CAGTGGAACT GCAACATCTA CTGGATCAGC TCCATCCTCA 
AGCACTTTGC CAGCAGCTTC TAGCCTTAAA ACCCCAGGAA CTTCTGCAAA TATGAATGGA 
CCCATTTCAA GAACAAGTTC TAGTATCCCT GCTAATAATC CTTTAGTTAC TCAGCTGCTC 
CAGGGCAAAG ATGTTCCCTT GGAGCAAATT CTGCCTAAGC CTCTCACCAA AATTGAAATG 
AAAACAGTTC CACTGACTAC AAAAGAGGAA AAAGGCATAG GGATATTCCC TGGTATCAGT 
GTAATGGAAA GTAGCAGCAG AGAGGAAGTT AATGGCAGGC AGGCCCATCT GGCTATCCCG 
CAGTTGGGGA AACCCTTGCA AAGTAAACAG CTTTCCCAGG TTCCAAGGCC AGTTTTCACT 
GCTAAGGACC GGAAGGACCC CTGCATTGAC ACTCACCAGT ACCGAGAAGG TCTAAGTAAA 
ACAACGCAAG ATCAGCTCTT TCAGACTCTT ATCCAGAGGG CTCAGAGACA GAGTGTTCTT 
TCATTTGTAC CGCCCTCTCA GTTCAACTTT GCTCACTCAG GTTTCCACTT GGAAGACATC 
TCCACAAGTC AGAAGTTCAT GCTGGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCAATG 
GCAGGTCACT ATTTACTTAA TATTTCCACC TATGGCAGGG GTACAGAGAA CATTAAAAGG 
ACCCATTCTG TAAATCCTGA TGATCGATTT TGTCTAAGTA GCCCCACTGA GGCGTTGAGA 
ATGGGGCATG CAGATTATAA AAACACAACT GGAGAAATCA GCAGCAAAGA AGATGAAAGT 
GATGAAGATC GCGTAGGTGA TGAACAGGAG CCTATTTCAG TGAAGGAGGA GCCATGGGCT 
TCCCAGAGTT CTGGCAGGCA TCCGCACCAT GGGGAAGCTT CATCTACCAA TGATTGTTTA 
GCTAGCAAGA ATGGTAAGAC TGAGGCACCA GTGAGTGAGC AAACCACTTT AGGCCAGGAG 
AATTACATAT TCTCTAGAGG CCAAGCATCT GATGAAAAGA GCCTGCCTAG AGATTTTATT 
CCGGCAGCAC ATAAACAGAT GACTCATGCT GTGAGAGGTA AGACAGTGTG TAGCAGCCCT 
GAGCTTTTCA ATTCTACTGC TCTTTCTCTT CCCGCAGACA GTCCCACACA TCAGCCTCTA 
CTCCTTCCAC CACTGCAAAC CCCCAAATTG TATGGAAGCC CCACACAGAT AGGGCCAAGC 
TATAGAGGCA TGATCAATGT CTCCACCTCA TCAGACATGG ACCATAACTC TGCTATACCA 
GGCAGCCAGG TATCTAGCAA TGTAGGGGAC GTCATGTCGT TTTCAGTGAC TGTCACTACC 
ATCCCTGCCA GTCAAGCAAT GAATCCCAGC AGCCATGGCC AGACAATTCC TGTTCAGACT 
TTTCCTGATG ACAATAGTAT CGAGGACACA CCATCAAAAT GTTACTGCCG GTTGAAAGCC 
ATGATCATGT GCAAAGGATG TGGAGCTTTC TGCCATGATG ATTGCATTGG CCCCTCCAAA 
CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_030246970.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102830.1 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X5, mRNA.

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CloneID	OMu71297
Clone ID Related Accession (Same CDS sequence)	XM_030246971.1 , XM_030246970.1
Accession Version	XM_030246970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCT CCAATCAACA CCTCTCTCTC AAGACTGTCA AAGCAGCCAG TGACTCTGTA 
CCTGCCAAAC CTGCCCTATG GGAAGGAAAG CAATCTGATG GACAGTCAAG CAGTCCCCAG 
AACTCAAATT CCAGCTTTTC TTCCTCAGTG AAAGTGGAAA GTTCTCTGCT AGGCTTGGGG 
AAGAAGTCTT TCCAGAGGTC TGACAGACTC CACACAAGAC AGATGAAAAG GACTAAATGT 
GCTGACATTG ATGTTGAGAC TCCTGATTCC ATTCTGGTTA ATACTAATCT TCGAGCACTG 
ATCAACAAGC ACACCTTTTC AGTTCTTCCT GGAGACTGCC AGCAGCGCCT GCTTTTGTTG 
CTTCCAGAGG TGGATCGACA GGTTGGTCCA GATGGCCTGA TGAAGTTGAA TGGCTCAGCC 
CTTAACAATG AGTTCTTCAC TTCTGCAGCC CAGGGCTGGA AGGAAAGGCT CTCAGAAGGT 
GAATTTACTC CTGAAATGCA GGTGAGAATT CGACAAGAGA TTGAGAAGGA GAAGAAAGTA 
GAGCTGTGGA AGGAACAATT CTTTGAAAAT TACTATGGTC AGAGTTCTGG CCTAAGCCTT 
GAAGATTCAC AGAAACTGAC AGCTTCCTCA AGTGATCCCA AAGCAAAGAA AACCCCAGCT 
GAGCAACCAA AATCCATACT TCCTTCAGAG GCCTCTCCTG TCAGAATTGT CCCAGTAGTT 
CCTCAGTCCG AGTGTAAAGA AGAAGCAGTG CAGATACCAT CACCATCCCA AAAGGAAGAA 
AATCAAGACG AGGCGAGGCC AGACTCCAAA TCCCCAGAAC CTGTTCTTGC ATCAGCTAGC 
AATACAAATG AGCTTATCAC CATGAAACCC ATCAAGAGCC CTAAGGATGA GGGTCTCTTA 
GAGCAGAAGC CTGTTGCCTG TGCTGAACAA GAATCTGAGA AAGAAAATCA TGTAACCACA 
ACTTCTCGTA ATAACAAAAG TGAGAACCAA GAAGCGTTAG CTATATCCCC AAGCAAATCT 
AAAAATGCTG GGTTACAAAA ACCCATAATA AAGCCTGTTG CAGAAGCCAG TCCACTGAAC 
CCTGATATGA AGATGCCACC AGCAACTGTT ACTGATCAGA TCCAAGAAAG TCTCAAGAGG 
AAATCTTCTC TTACTGATGA AGAGGCCACC AGTAGCTGGG AGAAAAGACC ACGTATCACT 
GAGAATCGCC AGCACCAGCA GCCATTTCAG GTCTCCCCAC AGCCTTTTCT TAATAGAGGG 
GACAGGGTCC AAGTGCGAAA AGTCCCACCT CTCAAGATCC CTGTCTCCAG AATCTCCCCG 
ATGCTGTTTT CTACATCACA GGTCTCTCCC AGGGCTCGTT TTCCAATCTC CATCACTAGT 
CCTTACAGAA CAGGAGCCAG AACTCTGGCA GACATCAAAG CAAAAGCCCA GTTGGTCAAA 
GCACAAAAGG CAGCAGCCGC AGCAGCTGCT GCAGCTGCTG CAGCCGCCTC AGTTGGAGGG 
ACCATTCCGG GACCTGGCCC TGGGGGTGGA CAAAGTCCAA GAGAGGGTGG TGAAAGGAAA 
ATTGCTGGAG GAGGAAGTGC AGGCTCAGAC CCAGTCAGTA CAAATGGAAA AGGTCCCACA 
CTGGAACTGG CCGGAACTGG AAGCAGGGGA GGTACGAGAG AACTTTTACC CTGTGGTCCT 
CAACCTGAGA CCAATATGCC AGGCCAAGCA CAGCCTCCTG GTATCTCTGG AGCACAACTA 
CAGCAAACCT CCTCAGTGCC TACGGGACTT GCCAGCAGTG GAGCATGCAC AAGTGTCCCA 
TTACCAGCCC ACATAGAAAT ATCAAACAGT GAAAAACCAA ACCTCCACAA GGCAACAGCC 
ACAGCAGCCT CTCCTTGCCA CTTGCAAGAC CCTAGGAGTT GCAGACTTGA AAAAGCTCTG 
TCTCCAACAG GGCCTCCTCT GATCTCAGGA GCCTCAACTG TTTATTTTGT AGCTGATGGC 
ACAGTTGAGC CCAAAGCAGG TTCTAATAAG AATGCACCAA AGCCTTCAGC CTTAGCAAAG 
ACAACTGCTC CTGCTCCACT AGATATGACT TCCTCTCCTG TGACAACAGC CAGTTTAGAA 
AAACTCCCTG TACCCCAGAT CAGTGGAACT GCAACATCTA CTGGATCAGC TCCATCCTCA 
AGCACTTTGC CAGCAGCTTC TAGCCTTAAA ACCCCAGGAA CTTCTGCAAA TATGAATGGA 
CCCATTTCAA GAACAAGTTC TAGTATCCCT GCTAATAATC CTTTAGTTAC TCAGCTGCTC 
CAGGGCAAAG ATGTTCCCTT GGAGCAAATT CTGCCTAAGC CTCTCACCAA AATTGAAATG 
AAAACAGTTC CACTGACTAC AAAAGAGGAA AAAGGCATAG GGATATTCCC TGGTATCAGT 
GTAATGGAAA GTAGCAGCAG AGAGGAAGTT AATGGCAGGC AGGCCCATCT GGCTATCCCG 
CAGTTGGGGA AACCCTTGCA AAGTAAACAG CTTTCCCAGG TTCCAAGGCC AGTTTTCACT 
GCTAAGGACC GGAAGGACCC CTGCATTGAC ACTCACCAGT ACCGAGAAGG TCTAAGTAAA 
ACAACGCAAG ATCAGCTCTT TCAGACTCTT ATCCAGAGGG CTCAGAGACA GAGTGTTCTT 
TCATTTGTAC CGCCCTCTCA GTTCAACTTT GCTCACTCAG GTTTCCACTT GGAAGACATC 
TCCACAAGTC AGAAGTTCAT GCTGGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCAATG 
GCAGGTCACT ATTTACTTAA TATTTCCACC TATGGCAGGG GTACAGAGAA CATTAAAAGG 
ACCCATTCTG TAAATCCTGA TGATCGATTT TGTCTAAGTA GCCCCACTGA GGCGTTGAGA 
ATGGGGCATG CAGATTATAA AAACACAACT GGAGAAATCA GCAGCAAAGA AGATGAAAGT 
GATGAAGATC GCGTAGGTGA TGAACAGGAG CCTATTTCAG TGAAGGAGGA GCCATGGGCT 
TCCCAGAGTT CTGGCAGGCA TCCGCACCAT GGGGAAGCTT CATCTACCAA TGATTGTTTA 
GCTAGCAAGA ATGGTAAGAC TGAGGCACCA GTGAGTGAGC AAACCACTTT AGGCCAGGAG 
AATTACATAT TCTCTAGAGG CCAAGCATCT GATGAAAAGA GCCTGCCTAG AGATTTTATT 
CCGGCAGCAC ATAAACAGAT GACTCATGCT GTGAGAGGTA AGACAGTGTG TAGCAGCCCT 
GAGCTTTTCA ATTCTACTGC TCTTTCTCTT CCCGCAGACA GTCCCACACA TCAGCCTCTA 
CTCCTTCCAC CACTGCAAAC CCCCAAATTG TATGGAAGCC CCACACAGAT AGGGCCAAGC 
TATAGAGGCA TGATCAATGT CTCCACCTCA TCAGACATGG ACCATAACTC TGCTATACCA 
GGCAGCCAGG TATCTAGCAA TGTAGGGGAC GTCATGTCGT TTTCAGTGAC TGTCACTACC 
ATCCCTGCCA GTCAAGCAAT GAATCCCAGC AGCCATGGCC AGACAATTCC TGTTCAGACT 
TTTCCTGATG ACAATAGTAT CGAGGACACA CCATCAAAAT GTTACTGCCG GTTGAAAGCC 
ATGATCATGT GCAAAGGATG TGGAGCTTTC TGCCATGATG ATTGCATTGG CCCCTCCAAA 
CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102830.1
CDS	264..3953
Translation	MSISNQHLSLKTVKAASDSVPAKPALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_030246970.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246970.1

ORF Insert Sequence:

ATGTCCATCT CCAATCAACA CCTCTCTCTC AAGACTGTCA AAGCAGCCAG TGACTCTGTA 
CCTGCCAAAC CTGCCCTATG GGAAGGAAAG CAATCTGATG GACAGTCAAG CAGTCCCCAG 
AACTCAAATT CCAGCTTTTC TTCCTCAGTG AAAGTGGAAA GTTCTCTGCT AGGCTTGGGG 
AAGAAGTCTT TCCAGAGGTC TGACAGACTC CACACAAGAC AGATGAAAAG GACTAAATGT 
GCTGACATTG ATGTTGAGAC TCCTGATTCC ATTCTGGTTA ATACTAATCT TCGAGCACTG 
ATCAACAAGC ACACCTTTTC AGTTCTTCCT GGAGACTGCC AGCAGCGCCT GCTTTTGTTG 
CTTCCAGAGG TGGATCGACA GGTTGGTCCA GATGGCCTGA TGAAGTTGAA TGGCTCAGCC 
CTTAACAATG AGTTCTTCAC TTCTGCAGCC CAGGGCTGGA AGGAAAGGCT CTCAGAAGGT 
GAATTTACTC CTGAAATGCA GGTGAGAATT CGACAAGAGA TTGAGAAGGA GAAGAAAGTA 
GAGCTGTGGA AGGAACAATT CTTTGAAAAT TACTATGGTC AGAGTTCTGG CCTAAGCCTT 
GAAGATTCAC AGAAACTGAC AGCTTCCTCA AGTGATCCCA AAGCAAAGAA AACCCCAGCT 
GAGCAACCAA AATCCATACT TCCTTCAGAG GCCTCTCCTG TCAGAATTGT CCCAGTAGTT 
CCTCAGTCCG AGTGTAAAGA AGAAGCAGTG CAGATACCAT CACCATCCCA AAAGGAAGAA 
AATCAAGACG AGGCGAGGCC AGACTCCAAA TCCCCAGAAC CTGTTCTTGC ATCAGCTAGC 
AATACAAATG AGCTTATCAC CATGAAACCC ATCAAGAGCC CTAAGGATGA GGGTCTCTTA 
GAGCAGAAGC CTGTTGCCTG TGCTGAACAA GAATCTGAGA AAGAAAATCA TGTAACCACA 
ACTTCTCGTA ATAACAAAAG TGAGAACCAA GAAGCGTTAG CTATATCCCC AAGCAAATCT 
AAAAATGCTG GGTTACAAAA ACCCATAATA AAGCCTGTTG CAGAAGCCAG TCCACTGAAC 
CCTGATATGA AGATGCCACC AGCAACTGTT ACTGATCAGA TCCAAGAAAG TCTCAAGAGG 
AAATCTTCTC TTACTGATGA AGAGGCCACC AGTAGCTGGG AGAAAAGACC ACGTATCACT 
GAGAATCGCC AGCACCAGCA GCCATTTCAG GTCTCCCCAC AGCCTTTTCT TAATAGAGGG 
GACAGGGTCC AAGTGCGAAA AGTCCCACCT CTCAAGATCC CTGTCTCCAG AATCTCCCCG 
ATGCTGTTTT CTACATCACA GGTCTCTCCC AGGGCTCGTT TTCCAATCTC CATCACTAGT 
CCTTACAGAA CAGGAGCCAG AACTCTGGCA GACATCAAAG CAAAAGCCCA GTTGGTCAAA 
GCACAAAAGG CAGCAGCCGC AGCAGCTGCT GCAGCTGCTG CAGCCGCCTC AGTTGGAGGG 
ACCATTCCGG GACCTGGCCC TGGGGGTGGA CAAAGTCCAA GAGAGGGTGG TGAAAGGAAA 
ATTGCTGGAG GAGGAAGTGC AGGCTCAGAC CCAGTCAGTA CAAATGGAAA AGGTCCCACA 
CTGGAACTGG CCGGAACTGG AAGCAGGGGA GGTACGAGAG AACTTTTACC CTGTGGTCCT 
CAACCTGAGA CCAATATGCC AGGCCAAGCA CAGCCTCCTG GTATCTCTGG AGCACAACTA 
CAGCAAACCT CCTCAGTGCC TACGGGACTT GCCAGCAGTG GAGCATGCAC AAGTGTCCCA 
TTACCAGCCC ACATAGAAAT ATCAAACAGT GAAAAACCAA ACCTCCACAA GGCAACAGCC 
ACAGCAGCCT CTCCTTGCCA CTTGCAAGAC CCTAGGAGTT GCAGACTTGA AAAAGCTCTG 
TCTCCAACAG GGCCTCCTCT GATCTCAGGA GCCTCAACTG TTTATTTTGT AGCTGATGGC 
ACAGTTGAGC CCAAAGCAGG TTCTAATAAG AATGCACCAA AGCCTTCAGC CTTAGCAAAG 
ACAACTGCTC CTGCTCCACT AGATATGACT TCCTCTCCTG TGACAACAGC CAGTTTAGAA 
AAACTCCCTG TACCCCAGAT CAGTGGAACT GCAACATCTA CTGGATCAGC TCCATCCTCA 
AGCACTTTGC CAGCAGCTTC TAGCCTTAAA ACCCCAGGAA CTTCTGCAAA TATGAATGGA 
CCCATTTCAA GAACAAGTTC TAGTATCCCT GCTAATAATC CTTTAGTTAC TCAGCTGCTC 
CAGGGCAAAG ATGTTCCCTT GGAGCAAATT CTGCCTAAGC CTCTCACCAA AATTGAAATG 
AAAACAGTTC CACTGACTAC AAAAGAGGAA AAAGGCATAG GGATATTCCC TGGTATCAGT 
GTAATGGAAA GTAGCAGCAG AGAGGAAGTT AATGGCAGGC AGGCCCATCT GGCTATCCCG 
CAGTTGGGGA AACCCTTGCA AAGTAAACAG CTTTCCCAGG TTCCAAGGCC AGTTTTCACT 
GCTAAGGACC GGAAGGACCC CTGCATTGAC ACTCACCAGT ACCGAGAAGG TCTAAGTAAA 
ACAACGCAAG ATCAGCTCTT TCAGACTCTT ATCCAGAGGG CTCAGAGACA GAGTGTTCTT 
TCATTTGTAC CGCCCTCTCA GTTCAACTTT GCTCACTCAG GTTTCCACTT GGAAGACATC 
TCCACAAGTC AGAAGTTCAT GCTGGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCAATG 
GCAGGTCACT ATTTACTTAA TATTTCCACC TATGGCAGGG GTACAGAGAA CATTAAAAGG 
ACCCATTCTG TAAATCCTGA TGATCGATTT TGTCTAAGTA GCCCCACTGA GGCGTTGAGA 
ATGGGGCATG CAGATTATAA AAACACAACT GGAGAAATCA GCAGCAAAGA AGATGAAAGT 
GATGAAGATC GCGTAGGTGA TGAACAGGAG CCTATTTCAG TGAAGGAGGA GCCATGGGCT 
TCCCAGAGTT CTGGCAGGCA TCCGCACCAT GGGGAAGCTT CATCTACCAA TGATTGTTTA 
GCTAGCAAGA ATGGTAAGAC TGAGGCACCA GTGAGTGAGC AAACCACTTT AGGCCAGGAG 
AATTACATAT TCTCTAGAGG CCAAGCATCT GATGAAAAGA GCCTGCCTAG AGATTTTATT 
CCGGCAGCAC ATAAACAGAT GACTCATGCT GTGAGAGGTA AGACAGTGTG TAGCAGCCCT 
GAGCTTTTCA ATTCTACTGC TCTTTCTCTT CCCGCAGACA GTCCCACACA TCAGCCTCTA 
CTCCTTCCAC CACTGCAAAC CCCCAAATTG TATGGAAGCC CCACACAGAT AGGGCCAAGC 
TATAGAGGCA TGATCAATGT CTCCACCTCA TCAGACATGG ACCATAACTC TGCTATACCA 
GGCAGCCAGG TATCTAGCAA TGTAGGGGAC GTCATGTCGT TTTCAGTGAC TGTCACTACC 
ATCCCTGCCA GTCAAGCAAT GAATCCCAGC AGCCATGGCC AGACAATTCC TGTTCAGACT 
TTTCCTGATG ACAATAGTAT CGAGGACACA CCATCAAAAT GTTACTGCCG GTTGAAAGCC 
ATGATCATGT GCAAAGGATG TGGAGCTTTC TGCCATGATG ATTGCATTGG CCCCTCCAAA 
CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_017315229.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170718.1 Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X1, mRNA.

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CloneID	OMu78329
Clone ID Related Accession (Same CDS sequence)	XM_017315229.1 , XM_030246967.1
Accession Version	XM_017315229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	putative Polycomb group protein ASXL2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG CCCTATGGGA AGGAAAGCAA 
TCTGATGGAC AGTCAAGCAG TCCCCAGAAC TCAAATTCCA GCTTTTCTTC CTCAGTGAAA 
GTGGAAAGTT CTCTGCTAGG CTTGGGGAAG AAGTCTTTCC AGAGGTCTGA CAGACTCCAC 
ACAAGACAGA TGAAAAGGAC TAAATGTGCT GACATTGATG TTGAGACTCC TGATTCCATT 
CTGGTTAATA CTAATCTTCG AGCACTGATC AACAAGCACA CCTTTTCAGT TCTTCCTGGA 
GACTGCCAGC AGCGCCTGCT TTTGTTGCTT CCAGAGGTGG ATCGACAGGT TGGTCCAGAT 
GGCCTGATGA AGTTGAATGG CTCAGCCCTT AACAATGAGT TCTTCACTTC TGCAGCCCAG 
GGCTGGAAGG AAAGGCTCTC AGAAGGTGAA TTTACTCCTG AAATGCAGGT GAGAATTCGA 
CAAGAGATTG AGAAGGAGAA GAAAGTAGAG CTGTGGAAGG AACAATTCTT TGAAAATTAC 
TATGGTCAGA GTTCTGGCCT AAGCCTTGAA GATTCACAGA AACTGACAGC TTCCTCAAGT 
GATCCCAAAG CAAAGAAAAC CCCAGCTGAG CAACCAAAAT CCATACTTCC TTCAGAGGCC 
TCTCCTGTCA GAATTGTCCC AGTAGTTCCT CAGTCCGAGT GTAAAGAAGA AGCAGTGCAG 
ATACCATCAC CATCCCAAAA GGAAGAAAAT CAAGACGAGG CGAGGCCAGA CTCCAAATCC 
CCAGAACCTG TTCTTGCATC AGCTAGCAAT ACAAATGAGC TTATCACCAT GAAACCCATC 
AAGAGCCCTA AGGATGAGGG TCTCTTAGAG CAGAAGCCTG TTGCCTGTGC TGAACAAGAA 
TCTGAGAAAG AAAATCATGT AACCACAACT TCTCGTAATA ACAAAAGTGA GAACCAAGAA 
GCGTTAGCTA TATCCCCAAG CAAATCTAAA AATGCTGGGT TACAAAAACC CATAATAAAG 
CCTGTTGCAG AAGCCAGTCC ACTGAACCCT GATATGAAGA TGCCACCAGC AACTGTTACT 
GATCAGATCC AAGAAAGTCT CAAGAGGAAA TCTTCTCTTA CTGATGAAGA GGCCACCAGT 
AGCTGGGAGA AAAGACCACG TATCACTGAG AATCGCCAGC ACCAGCAGCC ATTTCAGGTC 
TCCCCACAGC CTTTTCTTAA TAGAGGGGAC AGGGTCCAAG TGCGAAAAGT CCCACCTCTC 
AAGATCCCTG TCTCCAGAAT CTCCCCGATG CTGTTTTCTA CATCACAGGT CTCTCCCAGG 
GCTCGTTTTC CAATCTCCAT CACTAGTCCT TACAGAACAG GAGCCAGAAC TCTGGCAGAC 
ATCAAAGCAA AAGCCCAGTT GGTCAAAGCA CAAAAGGCAG CAGCCGCAGC AGCTGCTGCA 
GCTGCTGCAG CCGCCTCAGT TGGAGGGACC ATTCCGGGAC CTGGCCCTGG GGGTGGACAA 
AGTCCAAGAG AGGGTGGTGA AAGGAAAATT GCTGGAGGAG GAAGTGCAGG CTCAGACCCA 
GTCAGTACAA ATGGAAAAGG TCCCACACTG GAACTGGCCG GAACTGGAAG CAGGGGAGGT 
ACGAGAGAAC TTTTACCCTG TGGTCCTCAA CCTGAGACCA ATATGCCAGG CCAAGCACAG 
CCTCCTGGTA TCTCTGGAGC ACAACTACAG CAAACCTCCT CAGTGCCTAC GGGACTTGCC 
AGCAGTGGAG CATGCACAAG TGTCCCATTA CCAGCCCACA TAGAAATATC AAACAGTGAA 
AAACCAAACC TCCACAAGGC AACAGCCACA GCAGCCTCTC CTTGCCACTT GCAAGACCCT 
AGGAGTTGCA GACTTGAAAA AGCTCTGTCT CCAACAGGGC CTCCTCTGAT CTCAGGAGCC 
TCAACTGTTT ATTTTGTAGC TGATGGCACA GTTGAGCCCA AAGCAGGTTC TAATAAGAAT 
GCACCAAAGC CTTCAGCCTT AGCAAAGACA ACTGCTCCTG CTCCACTAGA TATGACTTCC 
TCTCCTGTGA CAACAGCCAG TTTAGAAAAA CTCCCTGTAC CCCAGATCAG TGGAACTGCA 
ACATCTACTG GATCAGCTCC ATCCTCAAGC ACTTTGCCAG CAGCTTCTAG CCTTAAAACC 
CCAGGAACTT CTGCAAATAT GAATGGACCC ATTTCAAGAA CAAGTTCTAG TATCCCTGCT 
AATAATCCTT TAGTTACTCA GCTGCTCCAG GGCAAAGATG TTCCCTTGGA GCAAATTCTG 
CCTAAGCCTC TCACCAAAAT TGAAATGAAA ACAGTTCCAC TGACTACAAA AGAGGAAAAA 
GGCATAGGGA TATTCCCTGG TATCAGTGTA ATGGAAAGTA GCAGCAGAGA GGAAGTTAAT 
GGCAGGCAGG CCCATCTGGC TATCCCGCAG TTGGGGAAAC CCTTGCAAAG TAAACAGCTT 
TCCCAGGTTC CAAGGCCAGT TTTCACTGCT AAGGACCGGA AGGACCCCTG CATTGACACT 
CACCAGTACC GAGAAGGTCT AAGTAAAACA ACGCAAGATC AGCTCTTTCA GACTCTTATC 
CAGAGGGCTC AGAGACAGAG TGTTCTTTCA TTTGTACCGC CCTCTCAGTT CAACTTTGCT 
CACTCAGGTT TCCACTTGGA AGACATCTCC ACAAGTCAGA AGTTCATGCT GGGTTTTGCT 
GGCAGAAGGA CATCCAAGCC TGCAATGGCA GGTCACTATT TACTTAATAT TTCCACCTAT 
GGCAGGGGTA CAGAGAACAT TAAAAGGACC CATTCTGTAA ATCCTGATGA TCGATTTTGT 
CTAAGTAGCC CCACTGAGGC GTTGAGAATG GGGCATGCAG ATTATAAAAA CACAACTGGA 
GAAATCAGCA GCAAAGAAGA TGAAAGTGAT GAAGATCGCG TAGGTGATGA ACAGGAGCCT 
ATTTCAGTGA AGGAGGAGCC ATGGGCTTCC CAGAGTTCTG GCAGGCATCC GCACCATGGG 
GAAGCTTCAT CTACCAATGA TTGTTTAGCT AGCAAGAATG GTAAGACTGA GGCACCAGTG 
AGTGAGCAAA CCACTTTAGG CCAGGAGAAT TACATATTCT CTAGAGGCCA AGCATCTGAT 
GAAAAGAGCC TGCCTAGAGA TTTTATTCCG GCAGCACATA AACAGATGAC TCATGCTGTG 
AGAGGTAAGA CAGTGTGTAG CAGCCCTGAG CTTTTCAATT CTACTGCTCT TTCTCTTCCC 
GCAGACAGTC CCACACATCA GCCTCTACTC CTTCCACCAC TGCAAACCCC CAAATTGTAT 
GGAAGCCCCA CACAGATAGG GCCAAGCTAT AGAGGCATGA TCAATGTCTC CACCTCATCA 
GACATGGACC ATAACTCTGC TATACCAGGC AGCCAGGTAT CTAGCAATGT AGGGGACGTC 
ATGTCGTTTT CAGTGACTGT CACTACCATC CCTGCCAGTC AAGCAATGAA TCCCAGCAGC 
CATGGCCAGA CAATTCCTGT TCAGACTTTT CCTGATGACA ATAGTATCGA GGACACACCA 
TCAAAATGTT ACTGCCGGTT GAAAGCCATG ATCATGTGCA AAGGATGTGG AGCTTTCTGC 
CATGATGATT GCATTGGCCC CTCCAAACTC TGTGTCTCCT GCCTTGTTGT TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170718.1
CDS	300..4556
Translation	MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEISSGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_017315229.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315229.1

ORF Insert Sequence:

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG CCCTATGGGA AGGAAAGCAA 
TCTGATGGAC AGTCAAGCAG TCCCCAGAAC TCAAATTCCA GCTTTTCTTC CTCAGTGAAA 
GTGGAAAGTT CTCTGCTAGG CTTGGGGAAG AAGTCTTTCC AGAGGTCTGA CAGACTCCAC 
ACAAGACAGA TGAAAAGGAC TAAATGTGCT GACATTGATG TTGAGACTCC TGATTCCATT 
CTGGTTAATA CTAATCTTCG AGCACTGATC AACAAGCACA CCTTTTCAGT TCTTCCTGGA 
GACTGCCAGC AGCGCCTGCT TTTGTTGCTT CCAGAGGTGG ATCGACAGGT TGGTCCAGAT 
GGCCTGATGA AGTTGAATGG CTCAGCCCTT AACAATGAGT TCTTCACTTC TGCAGCCCAG 
GGCTGGAAGG AAAGGCTCTC AGAAGGTGAA TTTACTCCTG AAATGCAGGT GAGAATTCGA 
CAAGAGATTG AGAAGGAGAA GAAAGTAGAG CTGTGGAAGG AACAATTCTT TGAAAATTAC 
TATGGTCAGA GTTCTGGCCT AAGCCTTGAA GATTCACAGA AACTGACAGC TTCCTCAAGT 
GATCCCAAAG CAAAGAAAAC CCCAGCTGAG CAACCAAAAT CCATACTTCC TTCAGAGGCC 
TCTCCTGTCA GAATTGTCCC AGTAGTTCCT CAGTCCGAGT GTAAAGAAGA AGCAGTGCAG 
ATACCATCAC CATCCCAAAA GGAAGAAAAT CAAGACGAGG CGAGGCCAGA CTCCAAATCC 
CCAGAACCTG TTCTTGCATC AGCTAGCAAT ACAAATGAGC TTATCACCAT GAAACCCATC 
AAGAGCCCTA AGGATGAGGG TCTCTTAGAG CAGAAGCCTG TTGCCTGTGC TGAACAAGAA 
TCTGAGAAAG AAAATCATGT AACCACAACT TCTCGTAATA ACAAAAGTGA GAACCAAGAA 
GCGTTAGCTA TATCCCCAAG CAAATCTAAA AATGCTGGGT TACAAAAACC CATAATAAAG 
CCTGTTGCAG AAGCCAGTCC ACTGAACCCT GATATGAAGA TGCCACCAGC AACTGTTACT 
GATCAGATCC AAGAAAGTCT CAAGAGGAAA TCTTCTCTTA CTGATGAAGA GGCCACCAGT 
AGCTGGGAGA AAAGACCACG TATCACTGAG AATCGCCAGC ACCAGCAGCC ATTTCAGGTC 
TCCCCACAGC CTTTTCTTAA TAGAGGGGAC AGGGTCCAAG TGCGAAAAGT CCCACCTCTC 
AAGATCCCTG TCTCCAGAAT CTCCCCGATG CTGTTTTCTA CATCACAGGT CTCTCCCAGG 
GCTCGTTTTC CAATCTCCAT CACTAGTCCT TACAGAACAG GAGCCAGAAC TCTGGCAGAC 
ATCAAAGCAA AAGCCCAGTT GGTCAAAGCA CAAAAGGCAG CAGCCGCAGC AGCTGCTGCA 
GCTGCTGCAG CCGCCTCAGT TGGAGGGACC ATTCCGGGAC CTGGCCCTGG GGGTGGACAA 
AGTCCAAGAG AGGGTGGTGA AAGGAAAATT GCTGGAGGAG GAAGTGCAGG CTCAGACCCA 
GTCAGTACAA ATGGAAAAGG TCCCACACTG GAACTGGCCG GAACTGGAAG CAGGGGAGGT 
ACGAGAGAAC TTTTACCCTG TGGTCCTCAA CCTGAGACCA ATATGCCAGG CCAAGCACAG 
CCTCCTGGTA TCTCTGGAGC ACAACTACAG CAAACCTCCT CAGTGCCTAC GGGACTTGCC 
AGCAGTGGAG CATGCACAAG TGTCCCATTA CCAGCCCACA TAGAAATATC AAACAGTGAA 
AAACCAAACC TCCACAAGGC AACAGCCACA GCAGCCTCTC CTTGCCACTT GCAAGACCCT 
AGGAGTTGCA GACTTGAAAA AGCTCTGTCT CCAACAGGGC CTCCTCTGAT CTCAGGAGCC 
TCAACTGTTT ATTTTGTAGC TGATGGCACA GTTGAGCCCA AAGCAGGTTC TAATAAGAAT 
GCACCAAAGC CTTCAGCCTT AGCAAAGACA ACTGCTCCTG CTCCACTAGA TATGACTTCC 
TCTCCTGTGA CAACAGCCAG TTTAGAAAAA CTCCCTGTAC CCCAGATCAG TGGAACTGCA 
ACATCTACTG GATCAGCTCC ATCCTCAAGC ACTTTGCCAG CAGCTTCTAG CCTTAAAACC 
CCAGGAACTT CTGCAAATAT GAATGGACCC ATTTCAAGAA CAAGTTCTAG TATCCCTGCT 
AATAATCCTT TAGTTACTCA GCTGCTCCAG GGCAAAGATG TTCCCTTGGA GCAAATTCTG 
CCTAAGCCTC TCACCAAAAT TGAAATGAAA ACAGTTCCAC TGACTACAAA AGAGGAAAAA 
GGCATAGGGA TATTCCCTGG TATCAGTGTA ATGGAAAGTA GCAGCAGAGA GGAAGTTAAT 
GGCAGGCAGG CCCATCTGGC TATCCCGCAG TTGGGGAAAC CCTTGCAAAG TAAACAGCTT 
TCCCAGGTTC CAAGGCCAGT TTTCACTGCT AAGGACCGGA AGGACCCCTG CATTGACACT 
CACCAGTACC GAGAAGGTCT AAGTAAAACA ACGCAAGATC AGCTCTTTCA GACTCTTATC 
CAGAGGGCTC AGAGACAGAG TGTTCTTTCA TTTGTACCGC CCTCTCAGTT CAACTTTGCT 
CACTCAGGTT TCCACTTGGA AGACATCTCC ACAAGTCAGA AGTTCATGCT GGGTTTTGCT 
GGCAGAAGGA CATCCAAGCC TGCAATGGCA GGTCACTATT TACTTAATAT TTCCACCTAT 
GGCAGGGGTA CAGAGAACAT TAAAAGGACC CATTCTGTAA ATCCTGATGA TCGATTTTGT 
CTAAGTAGCC CCACTGAGGC GTTGAGAATG GGGCATGCAG ATTATAAAAA CACAACTGGA 
GAAATCAGCA GCAAAGAAGA TGAAAGTGAT GAAGATCGCG TAGGTGATGA ACAGGAGCCT 
ATTTCAGTGA AGGAGGAGCC ATGGGCTTCC CAGAGTTCTG GCAGGCATCC GCACCATGGG 
GAAGCTTCAT CTACCAATGA TTGTTTAGCT AGCAAGAATG GTAAGACTGA GGCACCAGTG 
AGTGAGCAAA CCACTTTAGG CCAGGAGAAT TACATATTCT CTAGAGGCCA AGCATCTGAT 
GAAAAGAGCC TGCCTAGAGA TTTTATTCCG GCAGCACATA AACAGATGAC TCATGCTGTG 
AGAGGTAAGA CAGTGTGTAG CAGCCCTGAG CTTTTCAATT CTACTGCTCT TTCTCTTCCC 
GCAGACAGTC CCACACATCA GCCTCTACTC CTTCCACCAC TGCAAACCCC CAAATTGTAT 
GGAAGCCCCA CACAGATAGG GCCAAGCTAT AGAGGCATGA TCAATGTCTC CACCTCATCA 
GACATGGACC ATAACTCTGC TATACCAGGC AGCCAGGTAT CTAGCAATGT AGGGGACGTC 
ATGTCGTTTT CAGTGACTGT CACTACCATC CCTGCCAGTC AAGCAATGAA TCCCAGCAGC 
CATGGCCAGA CAATTCCTGT TCAGACTTTT CCTGATGACA ATAGTATCGA GGACACACCA 
TCAAAATGTT ACTGCCGGTT GAAAGCCATG ATCATGTGCA AAGGATGTGG AGCTTTCTGC 
CATGATGATT GCATTGGCCC CTCCAAACTC TGTGTCTCCT GCCTTGTTGT TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_030246967.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102827.1 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X1, mRNA.

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CloneID	OMu78329
Clone ID Related Accession (Same CDS sequence)	XM_017315229.1 , XM_030246967.1
Accession Version	XM_030246967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG CCCTATGGGA AGGAAAGCAA 
TCTGATGGAC AGTCAAGCAG TCCCCAGAAC TCAAATTCCA GCTTTTCTTC CTCAGTGAAA 
GTGGAAAGTT CTCTGCTAGG CTTGGGGAAG AAGTCTTTCC AGAGGTCTGA CAGACTCCAC 
ACAAGACAGA TGAAAAGGAC TAAATGTGCT GACATTGATG TTGAGACTCC TGATTCCATT 
CTGGTTAATA CTAATCTTCG AGCACTGATC AACAAGCACA CCTTTTCAGT TCTTCCTGGA 
GACTGCCAGC AGCGCCTGCT TTTGTTGCTT CCAGAGGTGG ATCGACAGGT TGGTCCAGAT 
GGCCTGATGA AGTTGAATGG CTCAGCCCTT AACAATGAGT TCTTCACTTC TGCAGCCCAG 
GGCTGGAAGG AAAGGCTCTC AGAAGGTGAA TTTACTCCTG AAATGCAGGT GAGAATTCGA 
CAAGAGATTG AGAAGGAGAA GAAAGTAGAG CTGTGGAAGG AACAATTCTT TGAAAATTAC 
TATGGTCAGA GTTCTGGCCT AAGCCTTGAA GATTCACAGA AACTGACAGC TTCCTCAAGT 
GATCCCAAAG CAAAGAAAAC CCCAGCTGAG CAACCAAAAT CCATACTTCC TTCAGAGGCC 
TCTCCTGTCA GAATTGTCCC AGTAGTTCCT CAGTCCGAGT GTAAAGAAGA AGCAGTGCAG 
ATACCATCAC CATCCCAAAA GGAAGAAAAT CAAGACGAGG CGAGGCCAGA CTCCAAATCC 
CCAGAACCTG TTCTTGCATC AGCTAGCAAT ACAAATGAGC TTATCACCAT GAAACCCATC 
AAGAGCCCTA AGGATGAGGG TCTCTTAGAG CAGAAGCCTG TTGCCTGTGC TGAACAAGAA 
TCTGAGAAAG AAAATCATGT AACCACAACT TCTCGTAATA ACAAAAGTGA GAACCAAGAA 
GCGTTAGCTA TATCCCCAAG CAAATCTAAA AATGCTGGGT TACAAAAACC CATAATAAAG 
CCTGTTGCAG AAGCCAGTCC ACTGAACCCT GATATGAAGA TGCCACCAGC AACTGTTACT 
GATCAGATCC AAGAAAGTCT CAAGAGGAAA TCTTCTCTTA CTGATGAAGA GGCCACCAGT 
AGCTGGGAGA AAAGACCACG TATCACTGAG AATCGCCAGC ACCAGCAGCC ATTTCAGGTC 
TCCCCACAGC CTTTTCTTAA TAGAGGGGAC AGGGTCCAAG TGCGAAAAGT CCCACCTCTC 
AAGATCCCTG TCTCCAGAAT CTCCCCGATG CTGTTTTCTA CATCACAGGT CTCTCCCAGG 
GCTCGTTTTC CAATCTCCAT CACTAGTCCT TACAGAACAG GAGCCAGAAC TCTGGCAGAC 
ATCAAAGCAA AAGCCCAGTT GGTCAAAGCA CAAAAGGCAG CAGCCGCAGC AGCTGCTGCA 
GCTGCTGCAG CCGCCTCAGT TGGAGGGACC ATTCCGGGAC CTGGCCCTGG GGGTGGACAA 
AGTCCAAGAG AGGGTGGTGA AAGGAAAATT GCTGGAGGAG GAAGTGCAGG CTCAGACCCA 
GTCAGTACAA ATGGAAAAGG TCCCACACTG GAACTGGCCG GAACTGGAAG CAGGGGAGGT 
ACGAGAGAAC TTTTACCCTG TGGTCCTCAA CCTGAGACCA ATATGCCAGG CCAAGCACAG 
CCTCCTGGTA TCTCTGGAGC ACAACTACAG CAAACCTCCT CAGTGCCTAC GGGACTTGCC 
AGCAGTGGAG CATGCACAAG TGTCCCATTA CCAGCCCACA TAGAAATATC AAACAGTGAA 
AAACCAAACC TCCACAAGGC AACAGCCACA GCAGCCTCTC CTTGCCACTT GCAAGACCCT 
AGGAGTTGCA GACTTGAAAA AGCTCTGTCT CCAACAGGGC CTCCTCTGAT CTCAGGAGCC 
TCAACTGTTT ATTTTGTAGC TGATGGCACA GTTGAGCCCA AAGCAGGTTC TAATAAGAAT 
GCACCAAAGC CTTCAGCCTT AGCAAAGACA ACTGCTCCTG CTCCACTAGA TATGACTTCC 
TCTCCTGTGA CAACAGCCAG TTTAGAAAAA CTCCCTGTAC CCCAGATCAG TGGAACTGCA 
ACATCTACTG GATCAGCTCC ATCCTCAAGC ACTTTGCCAG CAGCTTCTAG CCTTAAAACC 
CCAGGAACTT CTGCAAATAT GAATGGACCC ATTTCAAGAA CAAGTTCTAG TATCCCTGCT 
AATAATCCTT TAGTTACTCA GCTGCTCCAG GGCAAAGATG TTCCCTTGGA GCAAATTCTG 
CCTAAGCCTC TCACCAAAAT TGAAATGAAA ACAGTTCCAC TGACTACAAA AGAGGAAAAA 
GGCATAGGGA TATTCCCTGG TATCAGTGTA ATGGAAAGTA GCAGCAGAGA GGAAGTTAAT 
GGCAGGCAGG CCCATCTGGC TATCCCGCAG TTGGGGAAAC CCTTGCAAAG TAAACAGCTT 
TCCCAGGTTC CAAGGCCAGT TTTCACTGCT AAGGACCGGA AGGACCCCTG CATTGACACT 
CACCAGTACC GAGAAGGTCT AAGTAAAACA ACGCAAGATC AGCTCTTTCA GACTCTTATC 
CAGAGGGCTC AGAGACAGAG TGTTCTTTCA TTTGTACCGC CCTCTCAGTT CAACTTTGCT 
CACTCAGGTT TCCACTTGGA AGACATCTCC ACAAGTCAGA AGTTCATGCT GGGTTTTGCT 
GGCAGAAGGA CATCCAAGCC TGCAATGGCA GGTCACTATT TACTTAATAT TTCCACCTAT 
GGCAGGGGTA CAGAGAACAT TAAAAGGACC CATTCTGTAA ATCCTGATGA TCGATTTTGT 
CTAAGTAGCC CCACTGAGGC GTTGAGAATG GGGCATGCAG ATTATAAAAA CACAACTGGA 
GAAATCAGCA GCAAAGAAGA TGAAAGTGAT GAAGATCGCG TAGGTGATGA ACAGGAGCCT 
ATTTCAGTGA AGGAGGAGCC ATGGGCTTCC CAGAGTTCTG GCAGGCATCC GCACCATGGG 
GAAGCTTCAT CTACCAATGA TTGTTTAGCT AGCAAGAATG GTAAGACTGA GGCACCAGTG 
AGTGAGCAAA CCACTTTAGG CCAGGAGAAT TACATATTCT CTAGAGGCCA AGCATCTGAT 
GAAAAGAGCC TGCCTAGAGA TTTTATTCCG GCAGCACATA AACAGATGAC TCATGCTGTG 
AGAGGTAAGA CAGTGTGTAG CAGCCCTGAG CTTTTCAATT CTACTGCTCT TTCTCTTCCC 
GCAGACAGTC CCACACATCA GCCTCTACTC CTTCCACCAC TGCAAACCCC CAAATTGTAT 
GGAAGCCCCA CACAGATAGG GCCAAGCTAT AGAGGCATGA TCAATGTCTC CACCTCATCA 
GACATGGACC ATAACTCTGC TATACCAGGC AGCCAGGTAT CTAGCAATGT AGGGGACGTC 
ATGTCGTTTT CAGTGACTGT CACTACCATC CCTGCCAGTC AAGCAATGAA TCCCAGCAGC 
CATGGCCAGA CAATTCCTGT TCAGACTTTT CCTGATGACA ATAGTATCGA GGACACACCA 
TCAAAATGTT ACTGCCGGTT GAAAGCCATG ATCATGTGCA AAGGATGTGG AGCTTTCTGC 
CATGATGATT GCATTGGCCC CTCCAAACTC TGTGTCTCCT GCCTTGTTGT TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102827.1
CDS	267..4523
Translation	MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEISSGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_030246967.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246967.1

ORF Insert Sequence:

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG CCCTATGGGA AGGAAAGCAA 
TCTGATGGAC AGTCAAGCAG TCCCCAGAAC TCAAATTCCA GCTTTTCTTC CTCAGTGAAA 
GTGGAAAGTT CTCTGCTAGG CTTGGGGAAG AAGTCTTTCC AGAGGTCTGA CAGACTCCAC 
ACAAGACAGA TGAAAAGGAC TAAATGTGCT GACATTGATG TTGAGACTCC TGATTCCATT 
CTGGTTAATA CTAATCTTCG AGCACTGATC AACAAGCACA CCTTTTCAGT TCTTCCTGGA 
GACTGCCAGC AGCGCCTGCT TTTGTTGCTT CCAGAGGTGG ATCGACAGGT TGGTCCAGAT 
GGCCTGATGA AGTTGAATGG CTCAGCCCTT AACAATGAGT TCTTCACTTC TGCAGCCCAG 
GGCTGGAAGG AAAGGCTCTC AGAAGGTGAA TTTACTCCTG AAATGCAGGT GAGAATTCGA 
CAAGAGATTG AGAAGGAGAA GAAAGTAGAG CTGTGGAAGG AACAATTCTT TGAAAATTAC 
TATGGTCAGA GTTCTGGCCT AAGCCTTGAA GATTCACAGA AACTGACAGC TTCCTCAAGT 
GATCCCAAAG CAAAGAAAAC CCCAGCTGAG CAACCAAAAT CCATACTTCC TTCAGAGGCC 
TCTCCTGTCA GAATTGTCCC AGTAGTTCCT CAGTCCGAGT GTAAAGAAGA AGCAGTGCAG 
ATACCATCAC CATCCCAAAA GGAAGAAAAT CAAGACGAGG CGAGGCCAGA CTCCAAATCC 
CCAGAACCTG TTCTTGCATC AGCTAGCAAT ACAAATGAGC TTATCACCAT GAAACCCATC 
AAGAGCCCTA AGGATGAGGG TCTCTTAGAG CAGAAGCCTG TTGCCTGTGC TGAACAAGAA 
TCTGAGAAAG AAAATCATGT AACCACAACT TCTCGTAATA ACAAAAGTGA GAACCAAGAA 
GCGTTAGCTA TATCCCCAAG CAAATCTAAA AATGCTGGGT TACAAAAACC CATAATAAAG 
CCTGTTGCAG AAGCCAGTCC ACTGAACCCT GATATGAAGA TGCCACCAGC AACTGTTACT 
GATCAGATCC AAGAAAGTCT CAAGAGGAAA TCTTCTCTTA CTGATGAAGA GGCCACCAGT 
AGCTGGGAGA AAAGACCACG TATCACTGAG AATCGCCAGC ACCAGCAGCC ATTTCAGGTC 
TCCCCACAGC CTTTTCTTAA TAGAGGGGAC AGGGTCCAAG TGCGAAAAGT CCCACCTCTC 
AAGATCCCTG TCTCCAGAAT CTCCCCGATG CTGTTTTCTA CATCACAGGT CTCTCCCAGG 
GCTCGTTTTC CAATCTCCAT CACTAGTCCT TACAGAACAG GAGCCAGAAC TCTGGCAGAC 
ATCAAAGCAA AAGCCCAGTT GGTCAAAGCA CAAAAGGCAG CAGCCGCAGC AGCTGCTGCA 
GCTGCTGCAG CCGCCTCAGT TGGAGGGACC ATTCCGGGAC CTGGCCCTGG GGGTGGACAA 
AGTCCAAGAG AGGGTGGTGA AAGGAAAATT GCTGGAGGAG GAAGTGCAGG CTCAGACCCA 
GTCAGTACAA ATGGAAAAGG TCCCACACTG GAACTGGCCG GAACTGGAAG CAGGGGAGGT 
ACGAGAGAAC TTTTACCCTG TGGTCCTCAA CCTGAGACCA ATATGCCAGG CCAAGCACAG 
CCTCCTGGTA TCTCTGGAGC ACAACTACAG CAAACCTCCT CAGTGCCTAC GGGACTTGCC 
AGCAGTGGAG CATGCACAAG TGTCCCATTA CCAGCCCACA TAGAAATATC AAACAGTGAA 
AAACCAAACC TCCACAAGGC AACAGCCACA GCAGCCTCTC CTTGCCACTT GCAAGACCCT 
AGGAGTTGCA GACTTGAAAA AGCTCTGTCT CCAACAGGGC CTCCTCTGAT CTCAGGAGCC 
TCAACTGTTT ATTTTGTAGC TGATGGCACA GTTGAGCCCA AAGCAGGTTC TAATAAGAAT 
GCACCAAAGC CTTCAGCCTT AGCAAAGACA ACTGCTCCTG CTCCACTAGA TATGACTTCC 
TCTCCTGTGA CAACAGCCAG TTTAGAAAAA CTCCCTGTAC CCCAGATCAG TGGAACTGCA 
ACATCTACTG GATCAGCTCC ATCCTCAAGC ACTTTGCCAG CAGCTTCTAG CCTTAAAACC 
CCAGGAACTT CTGCAAATAT GAATGGACCC ATTTCAAGAA CAAGTTCTAG TATCCCTGCT 
AATAATCCTT TAGTTACTCA GCTGCTCCAG GGCAAAGATG TTCCCTTGGA GCAAATTCTG 
CCTAAGCCTC TCACCAAAAT TGAAATGAAA ACAGTTCCAC TGACTACAAA AGAGGAAAAA 
GGCATAGGGA TATTCCCTGG TATCAGTGTA ATGGAAAGTA GCAGCAGAGA GGAAGTTAAT 
GGCAGGCAGG CCCATCTGGC TATCCCGCAG TTGGGGAAAC CCTTGCAAAG TAAACAGCTT 
TCCCAGGTTC CAAGGCCAGT TTTCACTGCT AAGGACCGGA AGGACCCCTG CATTGACACT 
CACCAGTACC GAGAAGGTCT AAGTAAAACA ACGCAAGATC AGCTCTTTCA GACTCTTATC 
CAGAGGGCTC AGAGACAGAG TGTTCTTTCA TTTGTACCGC CCTCTCAGTT CAACTTTGCT 
CACTCAGGTT TCCACTTGGA AGACATCTCC ACAAGTCAGA AGTTCATGCT GGGTTTTGCT 
GGCAGAAGGA CATCCAAGCC TGCAATGGCA GGTCACTATT TACTTAATAT TTCCACCTAT 
GGCAGGGGTA CAGAGAACAT TAAAAGGACC CATTCTGTAA ATCCTGATGA TCGATTTTGT 
CTAAGTAGCC CCACTGAGGC GTTGAGAATG GGGCATGCAG ATTATAAAAA CACAACTGGA 
GAAATCAGCA GCAAAGAAGA TGAAAGTGAT GAAGATCGCG TAGGTGATGA ACAGGAGCCT 
ATTTCAGTGA AGGAGGAGCC ATGGGCTTCC CAGAGTTCTG GCAGGCATCC GCACCATGGG 
GAAGCTTCAT CTACCAATGA TTGTTTAGCT AGCAAGAATG GTAAGACTGA GGCACCAGTG 
AGTGAGCAAA CCACTTTAGG CCAGGAGAAT TACATATTCT CTAGAGGCCA AGCATCTGAT 
GAAAAGAGCC TGCCTAGAGA TTTTATTCCG GCAGCACATA AACAGATGAC TCATGCTGTG 
AGAGGTAAGA CAGTGTGTAG CAGCCCTGAG CTTTTCAATT CTACTGCTCT TTCTCTTCCC 
GCAGACAGTC CCACACATCA GCCTCTACTC CTTCCACCAC TGCAAACCCC CAAATTGTAT 
GGAAGCCCCA CACAGATAGG GCCAAGCTAT AGAGGCATGA TCAATGTCTC CACCTCATCA 
GACATGGACC ATAACTCTGC TATACCAGGC AGCCAGGTAT CTAGCAATGT AGGGGACGTC 
ATGTCGTTTT CAGTGACTGT CACTACCATC CCTGCCAGTC AAGCAATGAA TCCCAGCAGC 
CATGGCCAGA CAATTCCTGT TCAGACTTTT CCTGATGACA ATAGTATCGA GGACACACCA 
TCAAAATGTT ACTGCCGGTT GAAAGCCATG ATCATGTGCA AAGGATGTGG AGCTTTCTGC 
CATGATGATT GCATTGGCCC CTCCAAACTC TGTGTCTCCT GCCTTGTTGT TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_017315230.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170719.1 Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X3, mRNA.

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CloneID	OMu78330
Clone ID Related Accession (Same CDS sequence)	XM_017315230.1 , XM_030246968.1
Accession Version	XM_017315230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	putative Polycomb group protein ASXL2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG GACAGATGAA AAGGACTAAA 
TGTGCTGACA TTGATGTTGA GACTCCTGAT TCCATTCTGG TTAATACTAA TCTTCGAGCA 
CTGATCAACA AGCACACCTT TTCAGTTCTT CCTGGAGACT GCCAGCAGCG CCTGCTTTTG 
TTGCTTCCAG AGGTGGATCG ACAGGTTGGT CCAGATGGCC TGATGAAGTT GAATGGCTCA 
GCCCTTAACA ATGAGTTCTT CACTTCTGCA GCCCAGGGCT GGAAGGAAAG GCTCTCAGAA 
GGTGAATTTA CTCCTGAAAT GCAGGTGAGA ATTCGACAAG AGATTGAGAA GGAGAAGAAA 
GTAGAGCTGT GGAAGGAACA ATTCTTTGAA AATTACTATG GTCAGAGTTC TGGCCTAAGC 
CTTGAAGATT CACAGAAACT GACAGCTTCC TCAAGTGATC CCAAAGCAAA GAAAACCCCA 
GCTGAGCAAC CAAAATCCAT ACTTCCTTCA GAGGCCTCTC CTGTCAGAAT TGTCCCAGTA 
GTTCCTCAGT CCGAGTGTAA AGAAGAAGCA GTGCAGATAC CATCACCATC CCAAAAGGAA 
GAAAATCAAG ACGAGGCGAG GCCAGACTCC AAATCCCCAG AACCTGTTCT TGCATCAGCT 
AGCAATACAA ATGAGCTTAT CACCATGAAA CCCATCAAGA GCCCTAAGGA TGAGGGTCTC 
TTAGAGCAGA AGCCTGTTGC CTGTGCTGAA CAAGAATCTG AGAAAGAAAA TCATGTAACC 
ACAACTTCTC GTAATAACAA AAGTGAGAAC CAAGAAGCGT TAGCTATATC CCCAAGCAAA 
TCTAAAAATG CTGGGTTACA AAAACCCATA ATAAAGCCTG TTGCAGAAGC CAGTCCACTG 
AACCCTGATA TGAAGATGCC ACCAGCAACT GTTACTGATC AGATCCAAGA AAGTCTCAAG 
AGGAAATCTT CTCTTACTGA TGAAGAGGCC ACCAGTAGCT GGGAGAAAAG ACCACGTATC 
ACTGAGAATC GCCAGCACCA GCAGCCATTT CAGGTCTCCC CACAGCCTTT TCTTAATAGA 
GGGGACAGGG TCCAAGTGCG AAAAGTCCCA CCTCTCAAGA TCCCTGTCTC CAGAATCTCC 
CCGATGCTGT TTTCTACATC ACAGGTCTCT CCCAGGGCTC GTTTTCCAAT CTCCATCACT 
AGTCCTTACA GAACAGGAGC CAGAACTCTG GCAGACATCA AAGCAAAAGC CCAGTTGGTC 
AAAGCACAAA AGGCAGCAGC CGCAGCAGCT GCTGCAGCTG CTGCAGCCGC CTCAGTTGGA 
GGGACCATTC CGGGACCTGG CCCTGGGGGT GGACAAAGTC CAAGAGAGGG TGGTGAAAGG 
AAAATTGCTG GAGGAGGAAG TGCAGGCTCA GACCCAGTCA GTACAAATGG AAAAGGTCCC 
ACACTGGAAC TGGCCGGAAC TGGAAGCAGG GGAGGTACGA GAGAACTTTT ACCCTGTGGT 
CCTCAACCTG AGACCAATAT GCCAGGCCAA GCACAGCCTC CTGGTATCTC TGGAGCACAA 
CTACAGCAAA CCTCCTCAGT GCCTACGGGA CTTGCCAGCA GTGGAGCATG CACAAGTGTC 
CCATTACCAG CCCACATAGA AATATCAAAC AGTGAAAAAC CAAACCTCCA CAAGGCAACA 
GCCACAGCAG CCTCTCCTTG CCACTTGCAA GACCCTAGGA GTTGCAGACT TGAAAAAGCT 
CTGTCTCCAA CAGGGCCTCC TCTGATCTCA GGAGCCTCAA CTGTTTATTT TGTAGCTGAT 
GGCACAGTTG AGCCCAAAGC AGGTTCTAAT AAGAATGCAC CAAAGCCTTC AGCCTTAGCA 
AAGACAACTG CTCCTGCTCC ACTAGATATG ACTTCCTCTC CTGTGACAAC AGCCAGTTTA 
GAAAAACTCC CTGTACCCCA GATCAGTGGA ACTGCAACAT CTACTGGATC AGCTCCATCC 
TCAAGCACTT TGCCAGCAGC TTCTAGCCTT AAAACCCCAG GAACTTCTGC AAATATGAAT 
GGACCCATTT CAAGAACAAG TTCTAGTATC CCTGCTAATA ATCCTTTAGT TACTCAGCTG 
CTCCAGGGCA AAGATGTTCC CTTGGAGCAA ATTCTGCCTA AGCCTCTCAC CAAAATTGAA 
ATGAAAACAG TTCCACTGAC TACAAAAGAG GAAAAAGGCA TAGGGATATT CCCTGGTATC 
AGTGTAATGG AAAGTAGCAG CAGAGAGGAA GTTAATGGCA GGCAGGCCCA TCTGGCTATC 
CCGCAGTTGG GGAAACCCTT GCAAAGTAAA CAGCTTTCCC AGGTTCCAAG GCCAGTTTTC 
ACTGCTAAGG ACCGGAAGGA CCCCTGCATT GACACTCACC AGTACCGAGA AGGTCTAAGT 
AAAACAACGC AAGATCAGCT CTTTCAGACT CTTATCCAGA GGGCTCAGAG ACAGAGTGTT 
CTTTCATTTG TACCGCCCTC TCAGTTCAAC TTTGCTCACT CAGGTTTCCA CTTGGAAGAC 
ATCTCCACAA GTCAGAAGTT CATGCTGGGT TTTGCTGGCA GAAGGACATC CAAGCCTGCA 
ATGGCAGGTC ACTATTTACT TAATATTTCC ACCTATGGCA GGGGTACAGA GAACATTAAA 
AGGACCCATT CTGTAAATCC TGATGATCGA TTTTGTCTAA GTAGCCCCAC TGAGGCGTTG 
AGAATGGGGC ATGCAGATTA TAAAAACACA ACTGGAGAAA TCAGCAGCAA AGAAGATGAA 
AGTGATGAAG ATCGCGTAGG TGATGAACAG GAGCCTATTT CAGTGAAGGA GGAGCCATGG 
GCTTCCCAGA GTTCTGGCAG GCATCCGCAC CATGGGGAAG CTTCATCTAC CAATGATTGT 
TTAGCTAGCA AGAATGGTAA GACTGAGGCA CCAGTGAGTG AGCAAACCAC TTTAGGCCAG 
GAGAATTACA TATTCTCTAG AGGCCAAGCA TCTGATGAAA AGAGCCTGCC TAGAGATTTT 
ATTCCGGCAG CACATAAACA GATGACTCAT GCTGTGAGAG GTAAGACAGT GTGTAGCAGC 
CCTGAGCTTT TCAATTCTAC TGCTCTTTCT CTTCCCGCAG ACAGTCCCAC ACATCAGCCT 
CTACTCCTTC CACCACTGCA AACCCCCAAA TTGTATGGAA GCCCCACACA GATAGGGCCA 
AGCTATAGAG GCATGATCAA TGTCTCCACC TCATCAGACA TGGACCATAA CTCTGCTATA 
CCAGGCAGCC AGGTATCTAG CAATGTAGGG GACGTCATGT CGTTTTCAGT GACTGTCACT 
ACCATCCCTG CCAGTCAAGC AATGAATCCC AGCAGCCATG GCCAGACAAT TCCTGTTCAG 
ACTTTTCCTG ATGACAATAG TATCGAGGAC ACACCATCAA AATGTTACTG CCGGTTGAAA 
GCCATGATCA TGTGCAAAGG ATGTGGAGCT TTCTGCCATG ATGATTGCAT TGGCCCCTCC 
AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170719.1
CDS	300..4412
Translation	MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEISSGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPGQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_017315230.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315230.1

ORF Insert Sequence:

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG GACAGATGAA AAGGACTAAA 
TGTGCTGACA TTGATGTTGA GACTCCTGAT TCCATTCTGG TTAATACTAA TCTTCGAGCA 
CTGATCAACA AGCACACCTT TTCAGTTCTT CCTGGAGACT GCCAGCAGCG CCTGCTTTTG 
TTGCTTCCAG AGGTGGATCG ACAGGTTGGT CCAGATGGCC TGATGAAGTT GAATGGCTCA 
GCCCTTAACA ATGAGTTCTT CACTTCTGCA GCCCAGGGCT GGAAGGAAAG GCTCTCAGAA 
GGTGAATTTA CTCCTGAAAT GCAGGTGAGA ATTCGACAAG AGATTGAGAA GGAGAAGAAA 
GTAGAGCTGT GGAAGGAACA ATTCTTTGAA AATTACTATG GTCAGAGTTC TGGCCTAAGC 
CTTGAAGATT CACAGAAACT GACAGCTTCC TCAAGTGATC CCAAAGCAAA GAAAACCCCA 
GCTGAGCAAC CAAAATCCAT ACTTCCTTCA GAGGCCTCTC CTGTCAGAAT TGTCCCAGTA 
GTTCCTCAGT CCGAGTGTAA AGAAGAAGCA GTGCAGATAC CATCACCATC CCAAAAGGAA 
GAAAATCAAG ACGAGGCGAG GCCAGACTCC AAATCCCCAG AACCTGTTCT TGCATCAGCT 
AGCAATACAA ATGAGCTTAT CACCATGAAA CCCATCAAGA GCCCTAAGGA TGAGGGTCTC 
TTAGAGCAGA AGCCTGTTGC CTGTGCTGAA CAAGAATCTG AGAAAGAAAA TCATGTAACC 
ACAACTTCTC GTAATAACAA AAGTGAGAAC CAAGAAGCGT TAGCTATATC CCCAAGCAAA 
TCTAAAAATG CTGGGTTACA AAAACCCATA ATAAAGCCTG TTGCAGAAGC CAGTCCACTG 
AACCCTGATA TGAAGATGCC ACCAGCAACT GTTACTGATC AGATCCAAGA AAGTCTCAAG 
AGGAAATCTT CTCTTACTGA TGAAGAGGCC ACCAGTAGCT GGGAGAAAAG ACCACGTATC 
ACTGAGAATC GCCAGCACCA GCAGCCATTT CAGGTCTCCC CACAGCCTTT TCTTAATAGA 
GGGGACAGGG TCCAAGTGCG AAAAGTCCCA CCTCTCAAGA TCCCTGTCTC CAGAATCTCC 
CCGATGCTGT TTTCTACATC ACAGGTCTCT CCCAGGGCTC GTTTTCCAAT CTCCATCACT 
AGTCCTTACA GAACAGGAGC CAGAACTCTG GCAGACATCA AAGCAAAAGC CCAGTTGGTC 
AAAGCACAAA AGGCAGCAGC CGCAGCAGCT GCTGCAGCTG CTGCAGCCGC CTCAGTTGGA 
GGGACCATTC CGGGACCTGG CCCTGGGGGT GGACAAAGTC CAAGAGAGGG TGGTGAAAGG 
AAAATTGCTG GAGGAGGAAG TGCAGGCTCA GACCCAGTCA GTACAAATGG AAAAGGTCCC 
ACACTGGAAC TGGCCGGAAC TGGAAGCAGG GGAGGTACGA GAGAACTTTT ACCCTGTGGT 
CCTCAACCTG AGACCAATAT GCCAGGCCAA GCACAGCCTC CTGGTATCTC TGGAGCACAA 
CTACAGCAAA CCTCCTCAGT GCCTACGGGA CTTGCCAGCA GTGGAGCATG CACAAGTGTC 
CCATTACCAG CCCACATAGA AATATCAAAC AGTGAAAAAC CAAACCTCCA CAAGGCAACA 
GCCACAGCAG CCTCTCCTTG CCACTTGCAA GACCCTAGGA GTTGCAGACT TGAAAAAGCT 
CTGTCTCCAA CAGGGCCTCC TCTGATCTCA GGAGCCTCAA CTGTTTATTT TGTAGCTGAT 
GGCACAGTTG AGCCCAAAGC AGGTTCTAAT AAGAATGCAC CAAAGCCTTC AGCCTTAGCA 
AAGACAACTG CTCCTGCTCC ACTAGATATG ACTTCCTCTC CTGTGACAAC AGCCAGTTTA 
GAAAAACTCC CTGTACCCCA GATCAGTGGA ACTGCAACAT CTACTGGATC AGCTCCATCC 
TCAAGCACTT TGCCAGCAGC TTCTAGCCTT AAAACCCCAG GAACTTCTGC AAATATGAAT 
GGACCCATTT CAAGAACAAG TTCTAGTATC CCTGCTAATA ATCCTTTAGT TACTCAGCTG 
CTCCAGGGCA AAGATGTTCC CTTGGAGCAA ATTCTGCCTA AGCCTCTCAC CAAAATTGAA 
ATGAAAACAG TTCCACTGAC TACAAAAGAG GAAAAAGGCA TAGGGATATT CCCTGGTATC 
AGTGTAATGG AAAGTAGCAG CAGAGAGGAA GTTAATGGCA GGCAGGCCCA TCTGGCTATC 
CCGCAGTTGG GGAAACCCTT GCAAAGTAAA CAGCTTTCCC AGGTTCCAAG GCCAGTTTTC 
ACTGCTAAGG ACCGGAAGGA CCCCTGCATT GACACTCACC AGTACCGAGA AGGTCTAAGT 
AAAACAACGC AAGATCAGCT CTTTCAGACT CTTATCCAGA GGGCTCAGAG ACAGAGTGTT 
CTTTCATTTG TACCGCCCTC TCAGTTCAAC TTTGCTCACT CAGGTTTCCA CTTGGAAGAC 
ATCTCCACAA GTCAGAAGTT CATGCTGGGT TTTGCTGGCA GAAGGACATC CAAGCCTGCA 
ATGGCAGGTC ACTATTTACT TAATATTTCC ACCTATGGCA GGGGTACAGA GAACATTAAA 
AGGACCCATT CTGTAAATCC TGATGATCGA TTTTGTCTAA GTAGCCCCAC TGAGGCGTTG 
AGAATGGGGC ATGCAGATTA TAAAAACACA ACTGGAGAAA TCAGCAGCAA AGAAGATGAA 
AGTGATGAAG ATCGCGTAGG TGATGAACAG GAGCCTATTT CAGTGAAGGA GGAGCCATGG 
GCTTCCCAGA GTTCTGGCAG GCATCCGCAC CATGGGGAAG CTTCATCTAC CAATGATTGT 
TTAGCTAGCA AGAATGGTAA GACTGAGGCA CCAGTGAGTG AGCAAACCAC TTTAGGCCAG 
GAGAATTACA TATTCTCTAG AGGCCAAGCA TCTGATGAAA AGAGCCTGCC TAGAGATTTT 
ATTCCGGCAG CACATAAACA GATGACTCAT GCTGTGAGAG GTAAGACAGT GTGTAGCAGC 
CCTGAGCTTT TCAATTCTAC TGCTCTTTCT CTTCCCGCAG ACAGTCCCAC ACATCAGCCT 
CTACTCCTTC CACCACTGCA AACCCCCAAA TTGTATGGAA GCCCCACACA GATAGGGCCA 
AGCTATAGAG GCATGATCAA TGTCTCCACC TCATCAGACA TGGACCATAA CTCTGCTATA 
CCAGGCAGCC AGGTATCTAG CAATGTAGGG GACGTCATGT CGTTTTCAGT GACTGTCACT 
ACCATCCCTG CCAGTCAAGC AATGAATCCC AGCAGCCATG GCCAGACAAT TCCTGTTCAG 
ACTTTTCCTG ATGACAATAG TATCGAGGAC ACACCATCAA AATGTTACTG CCGGTTGAAA 
GCCATGATCA TGTGCAAAGG ATGTGGAGCT TTCTGCCATG ATGATTGCAT TGGCCCCTCC 
AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_030246968.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102828.1 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu78330
Clone ID Related Accession (Same CDS sequence)	XM_017315230.1 , XM_030246968.1
Accession Version	XM_030246968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG GACAGATGAA AAGGACTAAA 
TGTGCTGACA TTGATGTTGA GACTCCTGAT TCCATTCTGG TTAATACTAA TCTTCGAGCA 
CTGATCAACA AGCACACCTT TTCAGTTCTT CCTGGAGACT GCCAGCAGCG CCTGCTTTTG 
TTGCTTCCAG AGGTGGATCG ACAGGTTGGT CCAGATGGCC TGATGAAGTT GAATGGCTCA 
GCCCTTAACA ATGAGTTCTT CACTTCTGCA GCCCAGGGCT GGAAGGAAAG GCTCTCAGAA 
GGTGAATTTA CTCCTGAAAT GCAGGTGAGA ATTCGACAAG AGATTGAGAA GGAGAAGAAA 
GTAGAGCTGT GGAAGGAACA ATTCTTTGAA AATTACTATG GTCAGAGTTC TGGCCTAAGC 
CTTGAAGATT CACAGAAACT GACAGCTTCC TCAAGTGATC CCAAAGCAAA GAAAACCCCA 
GCTGAGCAAC CAAAATCCAT ACTTCCTTCA GAGGCCTCTC CTGTCAGAAT TGTCCCAGTA 
GTTCCTCAGT CCGAGTGTAA AGAAGAAGCA GTGCAGATAC CATCACCATC CCAAAAGGAA 
GAAAATCAAG ACGAGGCGAG GCCAGACTCC AAATCCCCAG AACCTGTTCT TGCATCAGCT 
AGCAATACAA ATGAGCTTAT CACCATGAAA CCCATCAAGA GCCCTAAGGA TGAGGGTCTC 
TTAGAGCAGA AGCCTGTTGC CTGTGCTGAA CAAGAATCTG AGAAAGAAAA TCATGTAACC 
ACAACTTCTC GTAATAACAA AAGTGAGAAC CAAGAAGCGT TAGCTATATC CCCAAGCAAA 
TCTAAAAATG CTGGGTTACA AAAACCCATA ATAAAGCCTG TTGCAGAAGC CAGTCCACTG 
AACCCTGATA TGAAGATGCC ACCAGCAACT GTTACTGATC AGATCCAAGA AAGTCTCAAG 
AGGAAATCTT CTCTTACTGA TGAAGAGGCC ACCAGTAGCT GGGAGAAAAG ACCACGTATC 
ACTGAGAATC GCCAGCACCA GCAGCCATTT CAGGTCTCCC CACAGCCTTT TCTTAATAGA 
GGGGACAGGG TCCAAGTGCG AAAAGTCCCA CCTCTCAAGA TCCCTGTCTC CAGAATCTCC 
CCGATGCTGT TTTCTACATC ACAGGTCTCT CCCAGGGCTC GTTTTCCAAT CTCCATCACT 
AGTCCTTACA GAACAGGAGC CAGAACTCTG GCAGACATCA AAGCAAAAGC CCAGTTGGTC 
AAAGCACAAA AGGCAGCAGC CGCAGCAGCT GCTGCAGCTG CTGCAGCCGC CTCAGTTGGA 
GGGACCATTC CGGGACCTGG CCCTGGGGGT GGACAAAGTC CAAGAGAGGG TGGTGAAAGG 
AAAATTGCTG GAGGAGGAAG TGCAGGCTCA GACCCAGTCA GTACAAATGG AAAAGGTCCC 
ACACTGGAAC TGGCCGGAAC TGGAAGCAGG GGAGGTACGA GAGAACTTTT ACCCTGTGGT 
CCTCAACCTG AGACCAATAT GCCAGGCCAA GCACAGCCTC CTGGTATCTC TGGAGCACAA 
CTACAGCAAA CCTCCTCAGT GCCTACGGGA CTTGCCAGCA GTGGAGCATG CACAAGTGTC 
CCATTACCAG CCCACATAGA AATATCAAAC AGTGAAAAAC CAAACCTCCA CAAGGCAACA 
GCCACAGCAG CCTCTCCTTG CCACTTGCAA GACCCTAGGA GTTGCAGACT TGAAAAAGCT 
CTGTCTCCAA CAGGGCCTCC TCTGATCTCA GGAGCCTCAA CTGTTTATTT TGTAGCTGAT 
GGCACAGTTG AGCCCAAAGC AGGTTCTAAT AAGAATGCAC CAAAGCCTTC AGCCTTAGCA 
AAGACAACTG CTCCTGCTCC ACTAGATATG ACTTCCTCTC CTGTGACAAC AGCCAGTTTA 
GAAAAACTCC CTGTACCCCA GATCAGTGGA ACTGCAACAT CTACTGGATC AGCTCCATCC 
TCAAGCACTT TGCCAGCAGC TTCTAGCCTT AAAACCCCAG GAACTTCTGC AAATATGAAT 
GGACCCATTT CAAGAACAAG TTCTAGTATC CCTGCTAATA ATCCTTTAGT TACTCAGCTG 
CTCCAGGGCA AAGATGTTCC CTTGGAGCAA ATTCTGCCTA AGCCTCTCAC CAAAATTGAA 
ATGAAAACAG TTCCACTGAC TACAAAAGAG GAAAAAGGCA TAGGGATATT CCCTGGTATC 
AGTGTAATGG AAAGTAGCAG CAGAGAGGAA GTTAATGGCA GGCAGGCCCA TCTGGCTATC 
CCGCAGTTGG GGAAACCCTT GCAAAGTAAA CAGCTTTCCC AGGTTCCAAG GCCAGTTTTC 
ACTGCTAAGG ACCGGAAGGA CCCCTGCATT GACACTCACC AGTACCGAGA AGGTCTAAGT 
AAAACAACGC AAGATCAGCT CTTTCAGACT CTTATCCAGA GGGCTCAGAG ACAGAGTGTT 
CTTTCATTTG TACCGCCCTC TCAGTTCAAC TTTGCTCACT CAGGTTTCCA CTTGGAAGAC 
ATCTCCACAA GTCAGAAGTT CATGCTGGGT TTTGCTGGCA GAAGGACATC CAAGCCTGCA 
ATGGCAGGTC ACTATTTACT TAATATTTCC ACCTATGGCA GGGGTACAGA GAACATTAAA 
AGGACCCATT CTGTAAATCC TGATGATCGA TTTTGTCTAA GTAGCCCCAC TGAGGCGTTG 
AGAATGGGGC ATGCAGATTA TAAAAACACA ACTGGAGAAA TCAGCAGCAA AGAAGATGAA 
AGTGATGAAG ATCGCGTAGG TGATGAACAG GAGCCTATTT CAGTGAAGGA GGAGCCATGG 
GCTTCCCAGA GTTCTGGCAG GCATCCGCAC CATGGGGAAG CTTCATCTAC CAATGATTGT 
TTAGCTAGCA AGAATGGTAA GACTGAGGCA CCAGTGAGTG AGCAAACCAC TTTAGGCCAG 
GAGAATTACA TATTCTCTAG AGGCCAAGCA TCTGATGAAA AGAGCCTGCC TAGAGATTTT 
ATTCCGGCAG CACATAAACA GATGACTCAT GCTGTGAGAG GTAAGACAGT GTGTAGCAGC 
CCTGAGCTTT TCAATTCTAC TGCTCTTTCT CTTCCCGCAG ACAGTCCCAC ACATCAGCCT 
CTACTCCTTC CACCACTGCA AACCCCCAAA TTGTATGGAA GCCCCACACA GATAGGGCCA 
AGCTATAGAG GCATGATCAA TGTCTCCACC TCATCAGACA TGGACCATAA CTCTGCTATA 
CCAGGCAGCC AGGTATCTAG CAATGTAGGG GACGTCATGT CGTTTTCAGT GACTGTCACT 
ACCATCCCTG CCAGTCAAGC AATGAATCCC AGCAGCCATG GCCAGACAAT TCCTGTTCAG 
ACTTTTCCTG ATGACAATAG TATCGAGGAC ACACCATCAA AATGTTACTG CCGGTTGAAA 
GCCATGATCA TGTGCAAAGG ATGTGGAGCT TTCTGCCATG ATGATTGCAT TGGCCCCTCC 
AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102828.1
CDS	300..4412
Translation	MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEISSGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPGQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_030246968.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246968.1

ORF Insert Sequence:

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG GACAGATGAA AAGGACTAAA 
TGTGCTGACA TTGATGTTGA GACTCCTGAT TCCATTCTGG TTAATACTAA TCTTCGAGCA 
CTGATCAACA AGCACACCTT TTCAGTTCTT CCTGGAGACT GCCAGCAGCG CCTGCTTTTG 
TTGCTTCCAG AGGTGGATCG ACAGGTTGGT CCAGATGGCC TGATGAAGTT GAATGGCTCA 
GCCCTTAACA ATGAGTTCTT CACTTCTGCA GCCCAGGGCT GGAAGGAAAG GCTCTCAGAA 
GGTGAATTTA CTCCTGAAAT GCAGGTGAGA ATTCGACAAG AGATTGAGAA GGAGAAGAAA 
GTAGAGCTGT GGAAGGAACA ATTCTTTGAA AATTACTATG GTCAGAGTTC TGGCCTAAGC 
CTTGAAGATT CACAGAAACT GACAGCTTCC TCAAGTGATC CCAAAGCAAA GAAAACCCCA 
GCTGAGCAAC CAAAATCCAT ACTTCCTTCA GAGGCCTCTC CTGTCAGAAT TGTCCCAGTA 
GTTCCTCAGT CCGAGTGTAA AGAAGAAGCA GTGCAGATAC CATCACCATC CCAAAAGGAA 
GAAAATCAAG ACGAGGCGAG GCCAGACTCC AAATCCCCAG AACCTGTTCT TGCATCAGCT 
AGCAATACAA ATGAGCTTAT CACCATGAAA CCCATCAAGA GCCCTAAGGA TGAGGGTCTC 
TTAGAGCAGA AGCCTGTTGC CTGTGCTGAA CAAGAATCTG AGAAAGAAAA TCATGTAACC 
ACAACTTCTC GTAATAACAA AAGTGAGAAC CAAGAAGCGT TAGCTATATC CCCAAGCAAA 
TCTAAAAATG CTGGGTTACA AAAACCCATA ATAAAGCCTG TTGCAGAAGC CAGTCCACTG 
AACCCTGATA TGAAGATGCC ACCAGCAACT GTTACTGATC AGATCCAAGA AAGTCTCAAG 
AGGAAATCTT CTCTTACTGA TGAAGAGGCC ACCAGTAGCT GGGAGAAAAG ACCACGTATC 
ACTGAGAATC GCCAGCACCA GCAGCCATTT CAGGTCTCCC CACAGCCTTT TCTTAATAGA 
GGGGACAGGG TCCAAGTGCG AAAAGTCCCA CCTCTCAAGA TCCCTGTCTC CAGAATCTCC 
CCGATGCTGT TTTCTACATC ACAGGTCTCT CCCAGGGCTC GTTTTCCAAT CTCCATCACT 
AGTCCTTACA GAACAGGAGC CAGAACTCTG GCAGACATCA AAGCAAAAGC CCAGTTGGTC 
AAAGCACAAA AGGCAGCAGC CGCAGCAGCT GCTGCAGCTG CTGCAGCCGC CTCAGTTGGA 
GGGACCATTC CGGGACCTGG CCCTGGGGGT GGACAAAGTC CAAGAGAGGG TGGTGAAAGG 
AAAATTGCTG GAGGAGGAAG TGCAGGCTCA GACCCAGTCA GTACAAATGG AAAAGGTCCC 
ACACTGGAAC TGGCCGGAAC TGGAAGCAGG GGAGGTACGA GAGAACTTTT ACCCTGTGGT 
CCTCAACCTG AGACCAATAT GCCAGGCCAA GCACAGCCTC CTGGTATCTC TGGAGCACAA 
CTACAGCAAA CCTCCTCAGT GCCTACGGGA CTTGCCAGCA GTGGAGCATG CACAAGTGTC 
CCATTACCAG CCCACATAGA AATATCAAAC AGTGAAAAAC CAAACCTCCA CAAGGCAACA 
GCCACAGCAG CCTCTCCTTG CCACTTGCAA GACCCTAGGA GTTGCAGACT TGAAAAAGCT 
CTGTCTCCAA CAGGGCCTCC TCTGATCTCA GGAGCCTCAA CTGTTTATTT TGTAGCTGAT 
GGCACAGTTG AGCCCAAAGC AGGTTCTAAT AAGAATGCAC CAAAGCCTTC AGCCTTAGCA 
AAGACAACTG CTCCTGCTCC ACTAGATATG ACTTCCTCTC CTGTGACAAC AGCCAGTTTA 
GAAAAACTCC CTGTACCCCA GATCAGTGGA ACTGCAACAT CTACTGGATC AGCTCCATCC 
TCAAGCACTT TGCCAGCAGC TTCTAGCCTT AAAACCCCAG GAACTTCTGC AAATATGAAT 
GGACCCATTT CAAGAACAAG TTCTAGTATC CCTGCTAATA ATCCTTTAGT TACTCAGCTG 
CTCCAGGGCA AAGATGTTCC CTTGGAGCAA ATTCTGCCTA AGCCTCTCAC CAAAATTGAA 
ATGAAAACAG TTCCACTGAC TACAAAAGAG GAAAAAGGCA TAGGGATATT CCCTGGTATC 
AGTGTAATGG AAAGTAGCAG CAGAGAGGAA GTTAATGGCA GGCAGGCCCA TCTGGCTATC 
CCGCAGTTGG GGAAACCCTT GCAAAGTAAA CAGCTTTCCC AGGTTCCAAG GCCAGTTTTC 
ACTGCTAAGG ACCGGAAGGA CCCCTGCATT GACACTCACC AGTACCGAGA AGGTCTAAGT 
AAAACAACGC AAGATCAGCT CTTTCAGACT CTTATCCAGA GGGCTCAGAG ACAGAGTGTT 
CTTTCATTTG TACCGCCCTC TCAGTTCAAC TTTGCTCACT CAGGTTTCCA CTTGGAAGAC 
ATCTCCACAA GTCAGAAGTT CATGCTGGGT TTTGCTGGCA GAAGGACATC CAAGCCTGCA 
ATGGCAGGTC ACTATTTACT TAATATTTCC ACCTATGGCA GGGGTACAGA GAACATTAAA 
AGGACCCATT CTGTAAATCC TGATGATCGA TTTTGTCTAA GTAGCCCCAC TGAGGCGTTG 
AGAATGGGGC ATGCAGATTA TAAAAACACA ACTGGAGAAA TCAGCAGCAA AGAAGATGAA 
AGTGATGAAG ATCGCGTAGG TGATGAACAG GAGCCTATTT CAGTGAAGGA GGAGCCATGG 
GCTTCCCAGA GTTCTGGCAG GCATCCGCAC CATGGGGAAG CTTCATCTAC CAATGATTGT 
TTAGCTAGCA AGAATGGTAA GACTGAGGCA CCAGTGAGTG AGCAAACCAC TTTAGGCCAG 
GAGAATTACA TATTCTCTAG AGGCCAAGCA TCTGATGAAA AGAGCCTGCC TAGAGATTTT 
ATTCCGGCAG CACATAAACA GATGACTCAT GCTGTGAGAG GTAAGACAGT GTGTAGCAGC 
CCTGAGCTTT TCAATTCTAC TGCTCTTTCT CTTCCCGCAG ACAGTCCCAC ACATCAGCCT 
CTACTCCTTC CACCACTGCA AACCCCCAAA TTGTATGGAA GCCCCACACA GATAGGGCCA 
AGCTATAGAG GCATGATCAA TGTCTCCACC TCATCAGACA TGGACCATAA CTCTGCTATA 
CCAGGCAGCC AGGTATCTAG CAATGTAGGG GACGTCATGT CGTTTTCAGT GACTGTCACT 
ACCATCCCTG CCAGTCAAGC AATGAATCCC AGCAGCCATG GCCAGACAAT TCCTGTTCAG 
ACTTTTCCTG ATGACAATAG TATCGAGGAC ACACCATCAA AATGTTACTG CCGGTTGAAA 
GCCATGATCA TGTGCAAAGG ATGTGGAGCT TTCTGCCATG ATGATTGCAT TGGCCCCTCC 
AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_017315232.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170721.1 Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X5, mRNA.

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CloneID	OMu78331
Clone ID Related Accession (Same CDS sequence)	XM_017315232.1 , XM_017315231.1
Accession Version	XM_017315232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCC CACAGGTGAA CTCAGGTTGT TGGGTTGGTA GCCACCTGTC TTGCTCAGCC 
TCAATGGGCT TTTGTGTATG CGCCTCTGTC ACCAGTCTTC ACCTATTTTT CCCCCTAGCC 
CTATGGGAAG GAAAGCAATC TGATGGACAG TCAAGCAGTC CCCAGAACTC AAATTCCAGC 
TTTTCTTCCT CAGTGAAAGT GGAAAGTTCT CTGCTAGGCT TGGGGAAGAA GTCTTTCCAG 
AGGTCTGACA GACTCCACAC AAGACAGATG AAAAGGACTA AATGTGCTGA CATTGATGTT 
GAGACTCCTG ATTCCATTCT GGTTAATACT AATCTTCGAG CACTGATCAA CAAGCACACC 
TTTTCAGTTC TTCCTGGAGA CTGCCAGCAG CGCCTGCTTT TGTTGCTTCC AGAGGTGGAT 
CGACAGGTTG GTCCAGATGG CCTGATGAAG TTGAATGGCT CAGCCCTTAA CAATGAGTTC 
TTCACTTCTG CAGCCCAGGG CTGGAAGGAA AGGCTCTCAG AAGGTGAATT TACTCCTGAA 
ATGCAGGTGA GAATTCGACA AGAGATTGAG AAGGAGAAGA AAGTAGAGCT GTGGAAGGAA 
CAATTCTTTG AAAATTACTA TGGTCAGAGT TCTGGCCTAA GCCTTGAAGA TTCACAGAAA 
CTGACAGCTT CCTCAAGTGA TCCCAAAGCA AAGAAAACCC CAGCTGAGCA ACCAAAATCC 
ATACTTCCTT CAGAGGCCTC TCCTGTCAGA ATTGTCCCAG TAGTTCCTCA GTCCGAGTGT 
AAAGAAGAAG CAGTGCAGAT ACCATCACCA TCCCAAAAGG AAGAAAATCA AGACGAGGCG 
AGGCCAGACT CCAAATCCCC AGAACCTGTT CTTGCATCAG CTAGCAATAC AAATGAGCTT 
ATCACCATGA AACCCATCAA GAGCCCTAAG GATGAGGGTC TCTTAGAGCA GAAGCCTGTT 
GCCTGTGCTG AACAAGAATC TGAGAAAGAA AATCATGTAA CCACAACTTC TCGTAATAAC 
AAAAGTGAGA ACCAAGAAGC GTTAGCTATA TCCCCAAGCA AATCTAAAAA TGCTGGGTTA 
CAAAAACCCA TAATAAAGCC TGTTGCAGAA GCCAGTCCAC TGAACCCTGA TATGAAGATG 
CCACCAGCAA CTGTTACTGA TCAGATCCAA GAAAGTCTCA AGAGGAAATC TTCTCTTACT 
GATGAAGAGG CCACCAGTAG CTGGGAGAAA AGACCACGTA TCACTGAGAA TCGCCAGCAC 
CAGCAGCCAT TTCAGGTCTC CCCACAGCCT TTTCTTAATA GAGGGGACAG GGTCCAAGTG 
CGAAAAGTCC CACCTCTCAA GATCCCTGTC TCCAGAATCT CCCCGATGCT GTTTTCTACA 
TCACAGGTCT CTCCCAGGGC TCGTTTTCCA ATCTCCATCA CTAGTCCTTA CAGAACAGGA 
GCCAGAACTC TGGCAGACAT CAAAGCAAAA GCCCAGTTGG TCAAAGCACA AAAGGCAGCA 
GCCGCAGCAG CTGCTGCAGC TGCTGCAGCC GCCTCAGTTG GAGGGACCAT TCCGGGACCT 
GGCCCTGGGG GTGGACAAAG TCCAAGAGAG GGTGGTGAAA GGAAAATTGC TGGAGGAGGA 
AGTGCAGGCT CAGACCCAGT CAGTACAAAT GGAAAAGGTC CCACACTGGA ACTGGCCGGA 
ACTGGAAGCA GGGGAGGTAC GAGAGAACTT TTACCCTGTG GTCCTCAACC TGAGACCAAT 
ATGCCAGGCC AAGCACAGCC TCCTGGTATC TCTGGAGCAC AACTACAGCA AACCTCCTCA 
GTGCCTACGG GACTTGCCAG CAGTGGAGCA TGCACAAGTG TCCCATTACC AGCCCACATA 
GAAATATCAA ACAGTGAAAA ACCAAACCTC CACAAGGCAA CAGCCACAGC AGCCTCTCCT 
TGCCACTTGC AAGACCCTAG GAGTTGCAGA CTTGAAAAAG CTCTGTCTCC AACAGGGCCT 
CCTCTGATCT CAGGAGCCTC AACTGTTTAT TTTGTAGCTG ATGGCACAGT TGAGCCCAAA 
GCAGGTTCTA ATAAGAATGC ACCAAAGCCT TCAGCCTTAG CAAAGACAAC TGCTCCTGCT 
CCACTAGATA TGACTTCCTC TCCTGTGACA ACAGCCAGTT TAGAAAAACT CCCTGTACCC 
CAGATCAGTG GAACTGCAAC ATCTACTGGA TCAGCTCCAT CCTCAAGCAC TTTGCCAGCA 
GCTTCTAGCC TTAAAACCCC AGGAACTTCT GCAAATATGA ATGGACCCAT TTCAAGAACA 
AGTTCTAGTA TCCCTGCTAA TAATCCTTTA GTTACTCAGC TGCTCCAGGG CAAAGATGTT 
CCCTTGGAGC AAATTCTGCC TAAGCCTCTC ACCAAAATTG AAATGAAAAC AGTTCCACTG 
ACTACAAAAG AGGAAAAAGG CATAGGGATA TTCCCTGGTA TCAGTGTAAT GGAAAGTAGC 
AGCAGAGAGG AAGTTAATGG CAGGCAGGCC CATCTGGCTA TCCCGCAGTT GGGGAAACCC 
TTGCAAAGTA AACAGCTTTC CCAGGTTCCA AGGCCAGTTT TCACTGCTAA GGACCGGAAG 
GACCCCTGCA TTGACACTCA CCAGTACCGA GAAGGTCTAA GTAAAACAAC GCAAGATCAG 
CTCTTTCAGA CTCTTATCCA GAGGGCTCAG AGACAGAGTG TTCTTTCATT TGTACCGCCC 
TCTCAGTTCA ACTTTGCTCA CTCAGGTTTC CACTTGGAAG ACATCTCCAC AAGTCAGAAG 
TTCATGCTGG GTTTTGCTGG CAGAAGGACA TCCAAGCCTG CAATGGCAGG TCACTATTTA 
CTTAATATTT CCACCTATGG CAGGGGTACA GAGAACATTA AAAGGACCCA TTCTGTAAAT 
CCTGATGATC GATTTTGTCT AAGTAGCCCC ACTGAGGCGT TGAGAATGGG GCATGCAGAT 
TATAAAAACA CAACTGGAGA AATCAGCAGC AAAGAAGATG AAAGTGATGA AGATCGCGTA 
GGTGATGAAC AGGAGCCTAT TTCAGTGAAG GAGGAGCCAT GGGCTTCCCA GAGTTCTGGC 
AGGCATCCGC ACCATGGGGA AGCTTCATCT ACCAATGATT GTTTAGCTAG CAAGAATGGT 
AAGACTGAGG CACCAGTGAG TGAGCAAACC ACTTTAGGCC AGGAGAATTA CATATTCTCT 
AGAGGCCAAG CATCTGATGA AAAGAGCCTG CCTAGAGATT TTATTCCGGC AGCACATAAA 
CAGATGACTC ATGCTGTGAG AGGTAAGACA GTGTGTAGCA GCCCTGAGCT TTTCAATTCT 
ACTGCTCTTT CTCTTCCCGC AGACAGTCCC ACACATCAGC CTCTACTCCT TCCACCACTG 
CAAACCCCCA AATTGTATGG AAGCCCCACA CAGATAGGGC CAAGCTATAG AGGCATGATC 
AATGTCTCCA CCTCATCAGA CATGGACCAT AACTCTGCTA TACCAGGCAG CCAGGTATCT 
AGCAATGTAG GGGACGTCAT GTCGTTTTCA GTGACTGTCA CTACCATCCC TGCCAGTCAA 
GCAATGAATC CCAGCAGCCA TGGCCAGACA ATTCCTGTTC AGACTTTTCC TGATGACAAT 
AGTATCGAGG ACACACCATC AAAATGTTAC TGCCGGTTGA AAGCCATGAT CATGTGCAAA 
GGATGTGGAG CTTTCTGCCA TGATGATTGC ATTGGCCCCT CCAAACTCTG TGTCTCCTGC 
CTTGTTGTTC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170721.1
CDS	1625..5359
Translation	MEGPQVNSGCWVGSHLSCSASMGFCVCASVTSLHLFFPLALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_017315232.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315232.1

ORF Insert Sequence:

ATGGAGGGCC CACAGGTGAA CTCAGGTTGT TGGGTTGGTA GCCACCTGTC TTGCTCAGCC 
TCAATGGGCT TTTGTGTATG CGCCTCTGTC ACCAGTCTTC ACCTATTTTT CCCCCTAGCC 
CTATGGGAAG GAAAGCAATC TGATGGACAG TCAAGCAGTC CCCAGAACTC AAATTCCAGC 
TTTTCTTCCT CAGTGAAAGT GGAAAGTTCT CTGCTAGGCT TGGGGAAGAA GTCTTTCCAG 
AGGTCTGACA GACTCCACAC AAGACAGATG AAAAGGACTA AATGTGCTGA CATTGATGTT 
GAGACTCCTG ATTCCATTCT GGTTAATACT AATCTTCGAG CACTGATCAA CAAGCACACC 
TTTTCAGTTC TTCCTGGAGA CTGCCAGCAG CGCCTGCTTT TGTTGCTTCC AGAGGTGGAT 
CGACAGGTTG GTCCAGATGG CCTGATGAAG TTGAATGGCT CAGCCCTTAA CAATGAGTTC 
TTCACTTCTG CAGCCCAGGG CTGGAAGGAA AGGCTCTCAG AAGGTGAATT TACTCCTGAA 
ATGCAGGTGA GAATTCGACA AGAGATTGAG AAGGAGAAGA AAGTAGAGCT GTGGAAGGAA 
CAATTCTTTG AAAATTACTA TGGTCAGAGT TCTGGCCTAA GCCTTGAAGA TTCACAGAAA 
CTGACAGCTT CCTCAAGTGA TCCCAAAGCA AAGAAAACCC CAGCTGAGCA ACCAAAATCC 
ATACTTCCTT CAGAGGCCTC TCCTGTCAGA ATTGTCCCAG TAGTTCCTCA GTCCGAGTGT 
AAAGAAGAAG CAGTGCAGAT ACCATCACCA TCCCAAAAGG AAGAAAATCA AGACGAGGCG 
AGGCCAGACT CCAAATCCCC AGAACCTGTT CTTGCATCAG CTAGCAATAC AAATGAGCTT 
ATCACCATGA AACCCATCAA GAGCCCTAAG GATGAGGGTC TCTTAGAGCA GAAGCCTGTT 
GCCTGTGCTG AACAAGAATC TGAGAAAGAA AATCATGTAA CCACAACTTC TCGTAATAAC 
AAAAGTGAGA ACCAAGAAGC GTTAGCTATA TCCCCAAGCA AATCTAAAAA TGCTGGGTTA 
CAAAAACCCA TAATAAAGCC TGTTGCAGAA GCCAGTCCAC TGAACCCTGA TATGAAGATG 
CCACCAGCAA CTGTTACTGA TCAGATCCAA GAAAGTCTCA AGAGGAAATC TTCTCTTACT 
GATGAAGAGG CCACCAGTAG CTGGGAGAAA AGACCACGTA TCACTGAGAA TCGCCAGCAC 
CAGCAGCCAT TTCAGGTCTC CCCACAGCCT TTTCTTAATA GAGGGGACAG GGTCCAAGTG 
CGAAAAGTCC CACCTCTCAA GATCCCTGTC TCCAGAATCT CCCCGATGCT GTTTTCTACA 
TCACAGGTCT CTCCCAGGGC TCGTTTTCCA ATCTCCATCA CTAGTCCTTA CAGAACAGGA 
GCCAGAACTC TGGCAGACAT CAAAGCAAAA GCCCAGTTGG TCAAAGCACA AAAGGCAGCA 
GCCGCAGCAG CTGCTGCAGC TGCTGCAGCC GCCTCAGTTG GAGGGACCAT TCCGGGACCT 
GGCCCTGGGG GTGGACAAAG TCCAAGAGAG GGTGGTGAAA GGAAAATTGC TGGAGGAGGA 
AGTGCAGGCT CAGACCCAGT CAGTACAAAT GGAAAAGGTC CCACACTGGA ACTGGCCGGA 
ACTGGAAGCA GGGGAGGTAC GAGAGAACTT TTACCCTGTG GTCCTCAACC TGAGACCAAT 
ATGCCAGGCC AAGCACAGCC TCCTGGTATC TCTGGAGCAC AACTACAGCA AACCTCCTCA 
GTGCCTACGG GACTTGCCAG CAGTGGAGCA TGCACAAGTG TCCCATTACC AGCCCACATA 
GAAATATCAA ACAGTGAAAA ACCAAACCTC CACAAGGCAA CAGCCACAGC AGCCTCTCCT 
TGCCACTTGC AAGACCCTAG GAGTTGCAGA CTTGAAAAAG CTCTGTCTCC AACAGGGCCT 
CCTCTGATCT CAGGAGCCTC AACTGTTTAT TTTGTAGCTG ATGGCACAGT TGAGCCCAAA 
GCAGGTTCTA ATAAGAATGC ACCAAAGCCT TCAGCCTTAG CAAAGACAAC TGCTCCTGCT 
CCACTAGATA TGACTTCCTC TCCTGTGACA ACAGCCAGTT TAGAAAAACT CCCTGTACCC 
CAGATCAGTG GAACTGCAAC ATCTACTGGA TCAGCTCCAT CCTCAAGCAC TTTGCCAGCA 
GCTTCTAGCC TTAAAACCCC AGGAACTTCT GCAAATATGA ATGGACCCAT TTCAAGAACA 
AGTTCTAGTA TCCCTGCTAA TAATCCTTTA GTTACTCAGC TGCTCCAGGG CAAAGATGTT 
CCCTTGGAGC AAATTCTGCC TAAGCCTCTC ACCAAAATTG AAATGAAAAC AGTTCCACTG 
ACTACAAAAG AGGAAAAAGG CATAGGGATA TTCCCTGGTA TCAGTGTAAT GGAAAGTAGC 
AGCAGAGAGG AAGTTAATGG CAGGCAGGCC CATCTGGCTA TCCCGCAGTT GGGGAAACCC 
TTGCAAAGTA AACAGCTTTC CCAGGTTCCA AGGCCAGTTT TCACTGCTAA GGACCGGAAG 
GACCCCTGCA TTGACACTCA CCAGTACCGA GAAGGTCTAA GTAAAACAAC GCAAGATCAG 
CTCTTTCAGA CTCTTATCCA GAGGGCTCAG AGACAGAGTG TTCTTTCATT TGTACCGCCC 
TCTCAGTTCA ACTTTGCTCA CTCAGGTTTC CACTTGGAAG ACATCTCCAC AAGTCAGAAG 
TTCATGCTGG GTTTTGCTGG CAGAAGGACA TCCAAGCCTG CAATGGCAGG TCACTATTTA 
CTTAATATTT CCACCTATGG CAGGGGTACA GAGAACATTA AAAGGACCCA TTCTGTAAAT 
CCTGATGATC GATTTTGTCT AAGTAGCCCC ACTGAGGCGT TGAGAATGGG GCATGCAGAT 
TATAAAAACA CAACTGGAGA AATCAGCAGC AAAGAAGATG AAAGTGATGA AGATCGCGTA 
GGTGATGAAC AGGAGCCTAT TTCAGTGAAG GAGGAGCCAT GGGCTTCCCA GAGTTCTGGC 
AGGCATCCGC ACCATGGGGA AGCTTCATCT ACCAATGATT GTTTAGCTAG CAAGAATGGT 
AAGACTGAGG CACCAGTGAG TGAGCAAACC ACTTTAGGCC AGGAGAATTA CATATTCTCT 
AGAGGCCAAG CATCTGATGA AAAGAGCCTG CCTAGAGATT TTATTCCGGC AGCACATAAA 
CAGATGACTC ATGCTGTGAG AGGTAAGACA GTGTGTAGCA GCCCTGAGCT TTTCAATTCT 
ACTGCTCTTT CTCTTCCCGC AGACAGTCCC ACACATCAGC CTCTACTCCT TCCACCACTG 
CAAACCCCCA AATTGTATGG AAGCCCCACA CAGATAGGGC CAAGCTATAG AGGCATGATC 
AATGTCTCCA CCTCATCAGA CATGGACCAT AACTCTGCTA TACCAGGCAG CCAGGTATCT 
AGCAATGTAG GGGACGTCAT GTCGTTTTCA GTGACTGTCA CTACCATCCC TGCCAGTCAA 
GCAATGAATC CCAGCAGCCA TGGCCAGACA ATTCCTGTTC AGACTTTTCC TGATGACAAT 
AGTATCGAGG ACACACCATC AAAATGTTAC TGCCGGTTGA AAGCCATGAT CATGTGCAAA 
GGATGTGGAG CTTTCTGCCA TGATGATTGC ATTGGCCCCT CCAAACTCTG TGTCTCCTGC 
CTTGTTGTTC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_017315231.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170720.1 Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X4, mRNA.

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CloneID	OMu78331
Clone ID Related Accession (Same CDS sequence)	XM_017315232.1 , XM_017315231.1
Accession Version	XM_017315231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCC CACAGGTGAA CTCAGGTTGT TGGGTTGGTA GCCACCTGTC TTGCTCAGCC 
TCAATGGGCT TTTGTGTATG CGCCTCTGTC ACCAGTCTTC ACCTATTTTT CCCCCTAGCC 
CTATGGGAAG GAAAGCAATC TGATGGACAG TCAAGCAGTC CCCAGAACTC AAATTCCAGC 
TTTTCTTCCT CAGTGAAAGT GGAAAGTTCT CTGCTAGGCT TGGGGAAGAA GTCTTTCCAG 
AGGTCTGACA GACTCCACAC AAGACAGATG AAAAGGACTA AATGTGCTGA CATTGATGTT 
GAGACTCCTG ATTCCATTCT GGTTAATACT AATCTTCGAG CACTGATCAA CAAGCACACC 
TTTTCAGTTC TTCCTGGAGA CTGCCAGCAG CGCCTGCTTT TGTTGCTTCC AGAGGTGGAT 
CGACAGGTTG GTCCAGATGG CCTGATGAAG TTGAATGGCT CAGCCCTTAA CAATGAGTTC 
TTCACTTCTG CAGCCCAGGG CTGGAAGGAA AGGCTCTCAG AAGGTGAATT TACTCCTGAA 
ATGCAGGTGA GAATTCGACA AGAGATTGAG AAGGAGAAGA AAGTAGAGCT GTGGAAGGAA 
CAATTCTTTG AAAATTACTA TGGTCAGAGT TCTGGCCTAA GCCTTGAAGA TTCACAGAAA 
CTGACAGCTT CCTCAAGTGA TCCCAAAGCA AAGAAAACCC CAGCTGAGCA ACCAAAATCC 
ATACTTCCTT CAGAGGCCTC TCCTGTCAGA ATTGTCCCAG TAGTTCCTCA GTCCGAGTGT 
AAAGAAGAAG CAGTGCAGAT ACCATCACCA TCCCAAAAGG AAGAAAATCA AGACGAGGCG 
AGGCCAGACT CCAAATCCCC AGAACCTGTT CTTGCATCAG CTAGCAATAC AAATGAGCTT 
ATCACCATGA AACCCATCAA GAGCCCTAAG GATGAGGGTC TCTTAGAGCA GAAGCCTGTT 
GCCTGTGCTG AACAAGAATC TGAGAAAGAA AATCATGTAA CCACAACTTC TCGTAATAAC 
AAAAGTGAGA ACCAAGAAGC GTTAGCTATA TCCCCAAGCA AATCTAAAAA TGCTGGGTTA 
CAAAAACCCA TAATAAAGCC TGTTGCAGAA GCCAGTCCAC TGAACCCTGA TATGAAGATG 
CCACCAGCAA CTGTTACTGA TCAGATCCAA GAAAGTCTCA AGAGGAAATC TTCTCTTACT 
GATGAAGAGG CCACCAGTAG CTGGGAGAAA AGACCACGTA TCACTGAGAA TCGCCAGCAC 
CAGCAGCCAT TTCAGGTCTC CCCACAGCCT TTTCTTAATA GAGGGGACAG GGTCCAAGTG 
CGAAAAGTCC CACCTCTCAA GATCCCTGTC TCCAGAATCT CCCCGATGCT GTTTTCTACA 
TCACAGGTCT CTCCCAGGGC TCGTTTTCCA ATCTCCATCA CTAGTCCTTA CAGAACAGGA 
GCCAGAACTC TGGCAGACAT CAAAGCAAAA GCCCAGTTGG TCAAAGCACA AAAGGCAGCA 
GCCGCAGCAG CTGCTGCAGC TGCTGCAGCC GCCTCAGTTG GAGGGACCAT TCCGGGACCT 
GGCCCTGGGG GTGGACAAAG TCCAAGAGAG GGTGGTGAAA GGAAAATTGC TGGAGGAGGA 
AGTGCAGGCT CAGACCCAGT CAGTACAAAT GGAAAAGGTC CCACACTGGA ACTGGCCGGA 
ACTGGAAGCA GGGGAGGTAC GAGAGAACTT TTACCCTGTG GTCCTCAACC TGAGACCAAT 
ATGCCAGGCC AAGCACAGCC TCCTGGTATC TCTGGAGCAC AACTACAGCA AACCTCCTCA 
GTGCCTACGG GACTTGCCAG CAGTGGAGCA TGCACAAGTG TCCCATTACC AGCCCACATA 
GAAATATCAA ACAGTGAAAA ACCAAACCTC CACAAGGCAA CAGCCACAGC AGCCTCTCCT 
TGCCACTTGC AAGACCCTAG GAGTTGCAGA CTTGAAAAAG CTCTGTCTCC AACAGGGCCT 
CCTCTGATCT CAGGAGCCTC AACTGTTTAT TTTGTAGCTG ATGGCACAGT TGAGCCCAAA 
GCAGGTTCTA ATAAGAATGC ACCAAAGCCT TCAGCCTTAG CAAAGACAAC TGCTCCTGCT 
CCACTAGATA TGACTTCCTC TCCTGTGACA ACAGCCAGTT TAGAAAAACT CCCTGTACCC 
CAGATCAGTG GAACTGCAAC ATCTACTGGA TCAGCTCCAT CCTCAAGCAC TTTGCCAGCA 
GCTTCTAGCC TTAAAACCCC AGGAACTTCT GCAAATATGA ATGGACCCAT TTCAAGAACA 
AGTTCTAGTA TCCCTGCTAA TAATCCTTTA GTTACTCAGC TGCTCCAGGG CAAAGATGTT 
CCCTTGGAGC AAATTCTGCC TAAGCCTCTC ACCAAAATTG AAATGAAAAC AGTTCCACTG 
ACTACAAAAG AGGAAAAAGG CATAGGGATA TTCCCTGGTA TCAGTGTAAT GGAAAGTAGC 
AGCAGAGAGG AAGTTAATGG CAGGCAGGCC CATCTGGCTA TCCCGCAGTT GGGGAAACCC 
TTGCAAAGTA AACAGCTTTC CCAGGTTCCA AGGCCAGTTT TCACTGCTAA GGACCGGAAG 
GACCCCTGCA TTGACACTCA CCAGTACCGA GAAGGTCTAA GTAAAACAAC GCAAGATCAG 
CTCTTTCAGA CTCTTATCCA GAGGGCTCAG AGACAGAGTG TTCTTTCATT TGTACCGCCC 
TCTCAGTTCA ACTTTGCTCA CTCAGGTTTC CACTTGGAAG ACATCTCCAC AAGTCAGAAG 
TTCATGCTGG GTTTTGCTGG CAGAAGGACA TCCAAGCCTG CAATGGCAGG TCACTATTTA 
CTTAATATTT CCACCTATGG CAGGGGTACA GAGAACATTA AAAGGACCCA TTCTGTAAAT 
CCTGATGATC GATTTTGTCT AAGTAGCCCC ACTGAGGCGT TGAGAATGGG GCATGCAGAT 
TATAAAAACA CAACTGGAGA AATCAGCAGC AAAGAAGATG AAAGTGATGA AGATCGCGTA 
GGTGATGAAC AGGAGCCTAT TTCAGTGAAG GAGGAGCCAT GGGCTTCCCA GAGTTCTGGC 
AGGCATCCGC ACCATGGGGA AGCTTCATCT ACCAATGATT GTTTAGCTAG CAAGAATGGT 
AAGACTGAGG CACCAGTGAG TGAGCAAACC ACTTTAGGCC AGGAGAATTA CATATTCTCT 
AGAGGCCAAG CATCTGATGA AAAGAGCCTG CCTAGAGATT TTATTCCGGC AGCACATAAA 
CAGATGACTC ATGCTGTGAG AGGTAAGACA GTGTGTAGCA GCCCTGAGCT TTTCAATTCT 
ACTGCTCTTT CTCTTCCCGC AGACAGTCCC ACACATCAGC CTCTACTCCT TCCACCACTG 
CAAACCCCCA AATTGTATGG AAGCCCCACA CAGATAGGGC CAAGCTATAG AGGCATGATC 
AATGTCTCCA CCTCATCAGA CATGGACCAT AACTCTGCTA TACCAGGCAG CCAGGTATCT 
AGCAATGTAG GGGACGTCAT GTCGTTTTCA GTGACTGTCA CTACCATCCC TGCCAGTCAA 
GCAATGAATC CCAGCAGCCA TGGCCAGACA ATTCCTGTTC AGACTTTTCC TGATGACAAT 
AGTATCGAGG ACACACCATC AAAATGTTAC TGCCGGTTGA AAGCCATGAT CATGTGCAAA 
GGATGTGGAG CTTTCTGCCA TGATGATTGC ATTGGCCCCT CCAAACTCTG TGTCTCCTGC 
CTTGTTGTTC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170720.1
CDS	1631..5365
Translation	MEGPQVNSGCWVGSHLSCSASMGFCVCASVTSLHLFFPLALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_017315231.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315231.1

ORF Insert Sequence:

ATGGAGGGCC CACAGGTGAA CTCAGGTTGT TGGGTTGGTA GCCACCTGTC TTGCTCAGCC 
TCAATGGGCT TTTGTGTATG CGCCTCTGTC ACCAGTCTTC ACCTATTTTT CCCCCTAGCC 
CTATGGGAAG GAAAGCAATC TGATGGACAG TCAAGCAGTC CCCAGAACTC AAATTCCAGC 
TTTTCTTCCT CAGTGAAAGT GGAAAGTTCT CTGCTAGGCT TGGGGAAGAA GTCTTTCCAG 
AGGTCTGACA GACTCCACAC AAGACAGATG AAAAGGACTA AATGTGCTGA CATTGATGTT 
GAGACTCCTG ATTCCATTCT GGTTAATACT AATCTTCGAG CACTGATCAA CAAGCACACC 
TTTTCAGTTC TTCCTGGAGA CTGCCAGCAG CGCCTGCTTT TGTTGCTTCC AGAGGTGGAT 
CGACAGGTTG GTCCAGATGG CCTGATGAAG TTGAATGGCT CAGCCCTTAA CAATGAGTTC 
TTCACTTCTG CAGCCCAGGG CTGGAAGGAA AGGCTCTCAG AAGGTGAATT TACTCCTGAA 
ATGCAGGTGA GAATTCGACA AGAGATTGAG AAGGAGAAGA AAGTAGAGCT GTGGAAGGAA 
CAATTCTTTG AAAATTACTA TGGTCAGAGT TCTGGCCTAA GCCTTGAAGA TTCACAGAAA 
CTGACAGCTT CCTCAAGTGA TCCCAAAGCA AAGAAAACCC CAGCTGAGCA ACCAAAATCC 
ATACTTCCTT CAGAGGCCTC TCCTGTCAGA ATTGTCCCAG TAGTTCCTCA GTCCGAGTGT 
AAAGAAGAAG CAGTGCAGAT ACCATCACCA TCCCAAAAGG AAGAAAATCA AGACGAGGCG 
AGGCCAGACT CCAAATCCCC AGAACCTGTT CTTGCATCAG CTAGCAATAC AAATGAGCTT 
ATCACCATGA AACCCATCAA GAGCCCTAAG GATGAGGGTC TCTTAGAGCA GAAGCCTGTT 
GCCTGTGCTG AACAAGAATC TGAGAAAGAA AATCATGTAA CCACAACTTC TCGTAATAAC 
AAAAGTGAGA ACCAAGAAGC GTTAGCTATA TCCCCAAGCA AATCTAAAAA TGCTGGGTTA 
CAAAAACCCA TAATAAAGCC TGTTGCAGAA GCCAGTCCAC TGAACCCTGA TATGAAGATG 
CCACCAGCAA CTGTTACTGA TCAGATCCAA GAAAGTCTCA AGAGGAAATC TTCTCTTACT 
GATGAAGAGG CCACCAGTAG CTGGGAGAAA AGACCACGTA TCACTGAGAA TCGCCAGCAC 
CAGCAGCCAT TTCAGGTCTC CCCACAGCCT TTTCTTAATA GAGGGGACAG GGTCCAAGTG 
CGAAAAGTCC CACCTCTCAA GATCCCTGTC TCCAGAATCT CCCCGATGCT GTTTTCTACA 
TCACAGGTCT CTCCCAGGGC TCGTTTTCCA ATCTCCATCA CTAGTCCTTA CAGAACAGGA 
GCCAGAACTC TGGCAGACAT CAAAGCAAAA GCCCAGTTGG TCAAAGCACA AAAGGCAGCA 
GCCGCAGCAG CTGCTGCAGC TGCTGCAGCC GCCTCAGTTG GAGGGACCAT TCCGGGACCT 
GGCCCTGGGG GTGGACAAAG TCCAAGAGAG GGTGGTGAAA GGAAAATTGC TGGAGGAGGA 
AGTGCAGGCT CAGACCCAGT CAGTACAAAT GGAAAAGGTC CCACACTGGA ACTGGCCGGA 
ACTGGAAGCA GGGGAGGTAC GAGAGAACTT TTACCCTGTG GTCCTCAACC TGAGACCAAT 
ATGCCAGGCC AAGCACAGCC TCCTGGTATC TCTGGAGCAC AACTACAGCA AACCTCCTCA 
GTGCCTACGG GACTTGCCAG CAGTGGAGCA TGCACAAGTG TCCCATTACC AGCCCACATA 
GAAATATCAA ACAGTGAAAA ACCAAACCTC CACAAGGCAA CAGCCACAGC AGCCTCTCCT 
TGCCACTTGC AAGACCCTAG GAGTTGCAGA CTTGAAAAAG CTCTGTCTCC AACAGGGCCT 
CCTCTGATCT CAGGAGCCTC AACTGTTTAT TTTGTAGCTG ATGGCACAGT TGAGCCCAAA 
GCAGGTTCTA ATAAGAATGC ACCAAAGCCT TCAGCCTTAG CAAAGACAAC TGCTCCTGCT 
CCACTAGATA TGACTTCCTC TCCTGTGACA ACAGCCAGTT TAGAAAAACT CCCTGTACCC 
CAGATCAGTG GAACTGCAAC ATCTACTGGA TCAGCTCCAT CCTCAAGCAC TTTGCCAGCA 
GCTTCTAGCC TTAAAACCCC AGGAACTTCT GCAAATATGA ATGGACCCAT TTCAAGAACA 
AGTTCTAGTA TCCCTGCTAA TAATCCTTTA GTTACTCAGC TGCTCCAGGG CAAAGATGTT 
CCCTTGGAGC AAATTCTGCC TAAGCCTCTC ACCAAAATTG AAATGAAAAC AGTTCCACTG 
ACTACAAAAG AGGAAAAAGG CATAGGGATA TTCCCTGGTA TCAGTGTAAT GGAAAGTAGC 
AGCAGAGAGG AAGTTAATGG CAGGCAGGCC CATCTGGCTA TCCCGCAGTT GGGGAAACCC 
TTGCAAAGTA AACAGCTTTC CCAGGTTCCA AGGCCAGTTT TCACTGCTAA GGACCGGAAG 
GACCCCTGCA TTGACACTCA CCAGTACCGA GAAGGTCTAA GTAAAACAAC GCAAGATCAG 
CTCTTTCAGA CTCTTATCCA GAGGGCTCAG AGACAGAGTG TTCTTTCATT TGTACCGCCC 
TCTCAGTTCA ACTTTGCTCA CTCAGGTTTC CACTTGGAAG ACATCTCCAC AAGTCAGAAG 
TTCATGCTGG GTTTTGCTGG CAGAAGGACA TCCAAGCCTG CAATGGCAGG TCACTATTTA 
CTTAATATTT CCACCTATGG CAGGGGTACA GAGAACATTA AAAGGACCCA TTCTGTAAAT 
CCTGATGATC GATTTTGTCT AAGTAGCCCC ACTGAGGCGT TGAGAATGGG GCATGCAGAT 
TATAAAAACA CAACTGGAGA AATCAGCAGC AAAGAAGATG AAAGTGATGA AGATCGCGTA 
GGTGATGAAC AGGAGCCTAT TTCAGTGAAG GAGGAGCCAT GGGCTTCCCA GAGTTCTGGC 
AGGCATCCGC ACCATGGGGA AGCTTCATCT ACCAATGATT GTTTAGCTAG CAAGAATGGT 
AAGACTGAGG CACCAGTGAG TGAGCAAACC ACTTTAGGCC AGGAGAATTA CATATTCTCT 
AGAGGCCAAG CATCTGATGA AAAGAGCCTG CCTAGAGATT TTATTCCGGC AGCACATAAA 
CAGATGACTC ATGCTGTGAG AGGTAAGACA GTGTGTAGCA GCCCTGAGCT TTTCAATTCT 
ACTGCTCTTT CTCTTCCCGC AGACAGTCCC ACACATCAGC CTCTACTCCT TCCACCACTG 
CAAACCCCCA AATTGTATGG AAGCCCCACA CAGATAGGGC CAAGCTATAG AGGCATGATC 
AATGTCTCCA CCTCATCAGA CATGGACCAT AACTCTGCTA TACCAGGCAG CCAGGTATCT 
AGCAATGTAG GGGACGTCAT GTCGTTTTCA GTGACTGTCA CTACCATCCC TGCCAGTCAA 
GCAATGAATC CCAGCAGCCA TGGCCAGACA ATTCCTGTTC AGACTTTTCC TGATGACAAT 
AGTATCGAGG ACACACCATC AAAATGTTAC TGCCGGTTGA AAGCCATGAT CATGTGCAAA 
GGATGTGGAG CTTTCTGCCA TGATGATTGC ATTGGCCCCT CCAAACTCTG TGTCTCCTGC 
CTTGTTGTTC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_030246969.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102829.1 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu86156
Clone ID Related Accession (Same CDS sequence)	XM_030246969.1
Accession Version	XM_030246969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCACA CAAACTCCCG AGGAGAAGAA GGCATCTTCT ATAAAGTTCC AGGTAGAATG 
GGCGTGTATA CTTTGAAGAA AGATGTGCCA GATGGAGTGA AAGAGCTGTC AGAAGGTTCA 
GAAGAAAGCA GTGATGGTCA GTCAGATTCT CAGAGCTCTG AGAACAGCAG CAGCAGTGAT 
GGTGGCAGCA ACAAAGAAGG GAGAAAGAGC AGGTGGAAAA GGAAAGTATC ATCTAGACTG 
TCACATCCAC CCTCTCCCCC GTCAGGCTGC CCATCACCCA CCATTCCAGC AAGTAAAGTC 
ATTTCTCCAT CACAGAAGCA CAGCAAAAAG GCATTAAAAC AGGCCTTGAA GCAGCAACAA 
CAGAAGAAGA AGCAGCAGCA ATGCAGACCA AGCATGTCCA TCTCCAATCA ACACCTCTCT 
CTCAAGACTG TCAAAGCAGC CAGTGACTCT GTACCTGCCA AACCTGCCCT ATGGGAAGGA 
AAGCAATCTG ATGGACAGTC AAGCAGTCCC CAGAACTCAA ATTCCAGCTT TTCTTCCTCA 
GTGAAAGTGG AAAGTTCTCT GCTAGGCTTG GGGAAGAAGT CTTTCCAGAG GTCTGACAGA 
CTCCACACAA GACAGATGAA AAGGACTAAA TGTGCTGACA TTGATGTTGA GACTCCTGAT 
TCCATTCTGG TTAATACTAA TCTTCGAGCA CTGATCAACA AGCACACCTT TTCAGTTCTT 
CCTGGAGACT GCCAGCAGCG CCTGCTTTTG TTGCTTCCAG AGGTGGATCG ACAGGTTGGT 
CCAGATGGCC TGATGAAGTT GAATGGCTCA GCCCTTAACA ATGAGTTCTT CACTTCTGCA 
GCCCAGGGCT GGAAGGAAAG GCTCTCAGAA GGTGAATTTA CTCCTGAAAT GCAGGTGAGA 
ATTCGACAAG AGATTGAGAA GGAGAAGAAA GTAGAGCTGT GGAAGGAACA ATTCTTTGAA 
AATTACTATG GTCAGAGTTC TGGCCTAAGC CTTGAAGATT CACAGAAACT GACAGCTTCC 
TCAAGTGATC CCAAAGCAAA GAAAACCCCA GCTGAGCAAC CAAAATCCAT ACTTCCTTCA 
GAGGCCTCTC CTGTCAGAAT TGTCCCAGTA GTTCCTCAGT CCGAGTGTAA AGAAGAAGCA 
GTGCAGATAC CATCACCATC CCAAAAGGAA GAAAATCAAG ACGAGGCGAG GCCAGACTCC 
AAATCCCCAG AACCTGTTCT TGCATCAGCT AGCAATACAA ATGAGCTTAT CACCATGAAA 
CCCATCAAGA GCCCTAAGGA TGAGGGTCTC TTAGAGCAGA AGCCTGTTGC CTGTGCTGAA 
CAAGAATCTG AGAAAGAAAA TCATGTAACC ACAACTTCTC GTAATAACAA AAGTGAGAAC 
CAAGAAGCGT TAGCTATATC CCCAAGCAAA TCTAAAAATG CTGGGTTACA AAAACCCATA 
ATAAAGCCTG TTGCAGAAGC CAGTCCACTG AACCCTGATA TGAAGATGCC ACCAGCAACT 
GTTACTGATC AGATCCAAGA AAGTCTCAAG AGGAAATCTT CTCTTACTGA TGAAGAGGCC 
ACCAGTAGCT GGGAGAAAAG ACCACGTATC ACTGAGAATC GCCAGCACCA GCAGCCATTT 
CAGGTCTCCC CACAGCCTTT TCTTAATAGA GGGGACAGGG TCCAAGTGCG AAAAGTCCCA 
CCTCTCAAGA TCCCTGTCTC CAGAATCTCC CCGATGCTGT TTTCTACATC ACAGGTCTCT 
CCCAGGGCTC GTTTTCCAAT CTCCATCACT AGTCCTTACA GAACAGGAGC CAGAACTCTG 
GCAGACATCA AAGCAAAAGC CCAGTTGGTC AAAGCACAAA AGGCAGCAGC CGCAGCAGCT 
GCTGCAGCTG CTGCAGCCGC CTCAGTTGGA GGGACCATTC CGGGACCTGG CCCTGGGGGT 
GGACAAAGTC CAAGAGAGGG TGGTGAAAGG AAAATTGCTG GAGGAGGAAG TGCAGGCTCA 
GACCCAGTCA GTACAAATGG AAAAGGTCCC ACACTGGAAC TGGCCGGAAC TGGAAGCAGG 
GGAGGTACGA GAGAACTTTT ACCCTGTGGT CCTCAACCTG AGACCAATAT GCCAGGCCAA 
GCACAGCCTC CTGGTATCTC TGGAGCACAA CTACAGCAAA CCTCCTCAGT GCCTACGGGA 
CTTGCCAGCA GTGGAGCATG CACAAGTGTC CCATTACCAG CCCACATAGA AATATCAAAC 
AGTGAAAAAC CAAACCTCCA CAAGGCAACA GCCACAGCAG CCTCTCCTTG CCACTTGCAA 
GACCCTAGGA GTTGCAGACT TGAAAAAGCT CTGTCTCCAA CAGGGCCTCC TCTGATCTCA 
GGAGCCTCAA CTGTTTATTT TGTAGCTGAT GGCACAGTTG AGCCCAAAGC AGGTTCTAAT 
AAGAATGCAC CAAAGCCTTC AGCCTTAGCA AAGACAACTG CTCCTGCTCC ACTAGATATG 
ACTTCCTCTC CTGTGACAAC AGCCAGTTTA GAAAAACTCC CTGTACCCCA GATCAGTGGA 
ACTGCAACAT CTACTGGATC AGCTCCATCC TCAAGCACTT TGCCAGCAGC TTCTAGCCTT 
AAAACCCCAG GAACTTCTGC AAATATGAAT GGACCCATTT CAAGAACAAG TTCTAGTATC 
CCTGCTAATA ATCCTTTAGT TACTCAGCTG CTCCAGGGCA AAGATGTTCC CTTGGAGCAA 
ATTCTGCCTA AGCCTCTCAC CAAAATTGAA ATGAAAACAG TTCCACTGAC TACAAAAGAG 
GAAAAAGGCA TAGGGATATT CCCTGGTATC AGTGTAATGG AAAGTAGCAG CAGAGAGGAA 
GTTAATGGCA GGCAGGCCCA TCTGGCTATC CCGCAGTTGG GGAAACCCTT GCAAAGTAAA 
CAGCTTTCCC AGGTTCCAAG GCCAGTTTTC ACTGCTAAGG ACCGGAAGGA CCCCTGCATT 
GACACTCACC AGTACCGAGA AGGTCTAAGT AAAACAACGC AAGATCAGCT CTTTCAGACT 
CTTATCCAGA GGGCTCAGAG ACAGAGTGTT CTTTCATTTG TACCGCCCTC TCAGTTCAAC 
TTTGCTCACT CAGGTTTCCA CTTGGAAGAC ATCTCCACAA GTCAGAAGTT CATGCTGGGT 
TTTGCTGGCA GAAGGACATC CAAGCCTGCA ATGGCAGGTC ACTATTTACT TAATATTTCC 
ACCTATGGCA GGGGTACAGA GAACATTAAA AGGACCCATT CTGTAAATCC TGATGATCGA 
TTTTGTCTAA GTAGCCCCAC TGAGGCGTTG AGAATGGGGC ATGCAGATTA TAAAAACACA 
ACTGGAGAAA TCAGCAGCAA AGAAGATGAA AGTGATGAAG ATCGCGTAGG TGATGAACAG 
GAGCCTATTT CAGTGAAGGA GGAGCCATGG GCTTCCCAGA GTTCTGGCAG GCATCCGCAC 
CATGGGGAAG CTTCATCTAC CAATGATTGT TTAGCTAGCA AGAATGGTAA GACTGAGGCA 
CCAGTGAGTG AGCAAACCAC TTTAGGCCAG GAGAATTACA TATTCTCTAG AGGCCAAGCA 
TCTGATGAAA AGAGCCTGCC TAGAGATTTT ATTCCGGCAG CACATAAACA GATGACTCAT 
GCTGTGAGAG GTAAGACAGT GTGTAGCAGC CCTGAGCTTT TCAATTCTAC TGCTCTTTCT 
CTTCCCGCAG ACAGTCCCAC ACATCAGCCT CTACTCCTTC CACCACTGCA AACCCCCAAA 
TTGTATGGAA GCCCCACACA GATAGGGCCA AGCTATAGAG GCATGATCAA TGTCTCCACC 
TCATCAGACA TGGACCATAA CTCTGCTATA CCAGGCAGCC AGGTATCTAG CAATGTAGGG 
GACGTCATGT CGTTTTCAGT GACTGTCACT ACCATCCCTG CCAGTCAAGC AATGAATCCC 
AGCAGCCATG GCCAGACAAT TCCTGTTCAG ACTTTTCCTG ATGACAATAG TATCGAGGAC 
ACACCATCAA AATGTTACTG CCGGTTGAAA GCCATGATCA TGTGCAAAGG ATGTGGAGCT 
TTCTGCCATG ATGATTGCAT TGGCCCCTCC AAACTCTGTG TCTCCTGCCT TGTTGTTCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102829.1
CDS	1131..5213
Translation	MLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_030246969.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246969.1

ORF Insert Sequence:

ATGCTGCACA CAAACTCCCG AGGAGAAGAA GGCATCTTCT ATAAAGTTCC AGGTAGAATG 
GGCGTGTATA CTTTGAAGAA AGATGTGCCA GATGGAGTGA AAGAGCTGTC AGAAGGTTCA 
GAAGAAAGCA GTGATGGTCA GTCAGATTCT CAGAGCTCTG AGAACAGCAG CAGCAGTGAT 
GGTGGCAGCA ACAAAGAAGG GAGAAAGAGC AGGTGGAAAA GGAAAGTATC ATCTAGACTG 
TCACATCCAC CCTCTCCCCC GTCAGGCTGC CCATCACCCA CCATTCCAGC AAGTAAAGTC 
ATTTCTCCAT CACAGAAGCA CAGCAAAAAG GCATTAAAAC AGGCCTTGAA GCAGCAACAA 
CAGAAGAAGA AGCAGCAGCA ATGCAGACCA AGCATGTCCA TCTCCAATCA ACACCTCTCT 
CTCAAGACTG TCAAAGCAGC CAGTGACTCT GTACCTGCCA AACCTGCCCT ATGGGAAGGA 
AAGCAATCTG ATGGACAGTC AAGCAGTCCC CAGAACTCAA ATTCCAGCTT TTCTTCCTCA 
GTGAAAGTGG AAAGTTCTCT GCTAGGCTTG GGGAAGAAGT CTTTCCAGAG GTCTGACAGA 
CTCCACACAA GACAGATGAA AAGGACTAAA TGTGCTGACA TTGATGTTGA GACTCCTGAT 
TCCATTCTGG TTAATACTAA TCTTCGAGCA CTGATCAACA AGCACACCTT TTCAGTTCTT 
CCTGGAGACT GCCAGCAGCG CCTGCTTTTG TTGCTTCCAG AGGTGGATCG ACAGGTTGGT 
CCAGATGGCC TGATGAAGTT GAATGGCTCA GCCCTTAACA ATGAGTTCTT CACTTCTGCA 
GCCCAGGGCT GGAAGGAAAG GCTCTCAGAA GGTGAATTTA CTCCTGAAAT GCAGGTGAGA 
ATTCGACAAG AGATTGAGAA GGAGAAGAAA GTAGAGCTGT GGAAGGAACA ATTCTTTGAA 
AATTACTATG GTCAGAGTTC TGGCCTAAGC CTTGAAGATT CACAGAAACT GACAGCTTCC 
TCAAGTGATC CCAAAGCAAA GAAAACCCCA GCTGAGCAAC CAAAATCCAT ACTTCCTTCA 
GAGGCCTCTC CTGTCAGAAT TGTCCCAGTA GTTCCTCAGT CCGAGTGTAA AGAAGAAGCA 
GTGCAGATAC CATCACCATC CCAAAAGGAA GAAAATCAAG ACGAGGCGAG GCCAGACTCC 
AAATCCCCAG AACCTGTTCT TGCATCAGCT AGCAATACAA ATGAGCTTAT CACCATGAAA 
CCCATCAAGA GCCCTAAGGA TGAGGGTCTC TTAGAGCAGA AGCCTGTTGC CTGTGCTGAA 
CAAGAATCTG AGAAAGAAAA TCATGTAACC ACAACTTCTC GTAATAACAA AAGTGAGAAC 
CAAGAAGCGT TAGCTATATC CCCAAGCAAA TCTAAAAATG CTGGGTTACA AAAACCCATA 
ATAAAGCCTG TTGCAGAAGC CAGTCCACTG AACCCTGATA TGAAGATGCC ACCAGCAACT 
GTTACTGATC AGATCCAAGA AAGTCTCAAG AGGAAATCTT CTCTTACTGA TGAAGAGGCC 
ACCAGTAGCT GGGAGAAAAG ACCACGTATC ACTGAGAATC GCCAGCACCA GCAGCCATTT 
CAGGTCTCCC CACAGCCTTT TCTTAATAGA GGGGACAGGG TCCAAGTGCG AAAAGTCCCA 
CCTCTCAAGA TCCCTGTCTC CAGAATCTCC CCGATGCTGT TTTCTACATC ACAGGTCTCT 
CCCAGGGCTC GTTTTCCAAT CTCCATCACT AGTCCTTACA GAACAGGAGC CAGAACTCTG 
GCAGACATCA AAGCAAAAGC CCAGTTGGTC AAAGCACAAA AGGCAGCAGC CGCAGCAGCT 
GCTGCAGCTG CTGCAGCCGC CTCAGTTGGA GGGACCATTC CGGGACCTGG CCCTGGGGGT 
GGACAAAGTC CAAGAGAGGG TGGTGAAAGG AAAATTGCTG GAGGAGGAAG TGCAGGCTCA 
GACCCAGTCA GTACAAATGG AAAAGGTCCC ACACTGGAAC TGGCCGGAAC TGGAAGCAGG 
GGAGGTACGA GAGAACTTTT ACCCTGTGGT CCTCAACCTG AGACCAATAT GCCAGGCCAA 
GCACAGCCTC CTGGTATCTC TGGAGCACAA CTACAGCAAA CCTCCTCAGT GCCTACGGGA 
CTTGCCAGCA GTGGAGCATG CACAAGTGTC CCATTACCAG CCCACATAGA AATATCAAAC 
AGTGAAAAAC CAAACCTCCA CAAGGCAACA GCCACAGCAG CCTCTCCTTG CCACTTGCAA 
GACCCTAGGA GTTGCAGACT TGAAAAAGCT CTGTCTCCAA CAGGGCCTCC TCTGATCTCA 
GGAGCCTCAA CTGTTTATTT TGTAGCTGAT GGCACAGTTG AGCCCAAAGC AGGTTCTAAT 
AAGAATGCAC CAAAGCCTTC AGCCTTAGCA AAGACAACTG CTCCTGCTCC ACTAGATATG 
ACTTCCTCTC CTGTGACAAC AGCCAGTTTA GAAAAACTCC CTGTACCCCA GATCAGTGGA 
ACTGCAACAT CTACTGGATC AGCTCCATCC TCAAGCACTT TGCCAGCAGC TTCTAGCCTT 
AAAACCCCAG GAACTTCTGC AAATATGAAT GGACCCATTT CAAGAACAAG TTCTAGTATC 
CCTGCTAATA ATCCTTTAGT TACTCAGCTG CTCCAGGGCA AAGATGTTCC CTTGGAGCAA 
ATTCTGCCTA AGCCTCTCAC CAAAATTGAA ATGAAAACAG TTCCACTGAC TACAAAAGAG 
GAAAAAGGCA TAGGGATATT CCCTGGTATC AGTGTAATGG AAAGTAGCAG CAGAGAGGAA 
GTTAATGGCA GGCAGGCCCA TCTGGCTATC CCGCAGTTGG GGAAACCCTT GCAAAGTAAA 
CAGCTTTCCC AGGTTCCAAG GCCAGTTTTC ACTGCTAAGG ACCGGAAGGA CCCCTGCATT 
GACACTCACC AGTACCGAGA AGGTCTAAGT AAAACAACGC AAGATCAGCT CTTTCAGACT 
CTTATCCAGA GGGCTCAGAG ACAGAGTGTT CTTTCATTTG TACCGCCCTC TCAGTTCAAC 
TTTGCTCACT CAGGTTTCCA CTTGGAAGAC ATCTCCACAA GTCAGAAGTT CATGCTGGGT 
TTTGCTGGCA GAAGGACATC CAAGCCTGCA ATGGCAGGTC ACTATTTACT TAATATTTCC 
ACCTATGGCA GGGGTACAGA GAACATTAAA AGGACCCATT CTGTAAATCC TGATGATCGA 
TTTTGTCTAA GTAGCCCCAC TGAGGCGTTG AGAATGGGGC ATGCAGATTA TAAAAACACA 
ACTGGAGAAA TCAGCAGCAA AGAAGATGAA AGTGATGAAG ATCGCGTAGG TGATGAACAG 
GAGCCTATTT CAGTGAAGGA GGAGCCATGG GCTTCCCAGA GTTCTGGCAG GCATCCGCAC 
CATGGGGAAG CTTCATCTAC CAATGATTGT TTAGCTAGCA AGAATGGTAA GACTGAGGCA 
CCAGTGAGTG AGCAAACCAC TTTAGGCCAG GAGAATTACA TATTCTCTAG AGGCCAAGCA 
TCTGATGAAA AGAGCCTGCC TAGAGATTTT ATTCCGGCAG CACATAAACA GATGACTCAT 
GCTGTGAGAG GTAAGACAGT GTGTAGCAGC CCTGAGCTTT TCAATTCTAC TGCTCTTTCT 
CTTCCCGCAG ACAGTCCCAC ACATCAGCCT CTACTCCTTC CACCACTGCA AACCCCCAAA 
TTGTATGGAA GCCCCACACA GATAGGGCCA AGCTATAGAG GCATGATCAA TGTCTCCACC 
TCATCAGACA TGGACCATAA CTCTGCTATA CCAGGCAGCC AGGTATCTAG CAATGTAGGG 
GACGTCATGT CGTTTTCAGT GACTGTCACT ACCATCCCTG CCAGTCAAGC AATGAATCCC 
AGCAGCCATG GCCAGACAAT TCCTGTTCAG ACTTTTCCTG ATGACAATAG TATCGAGGAC 
ACACCATCAA AATGTTACTG CCGGTTGAAA GCCATGATCA TGTGCAAAGG ATGTGGAGCT 
TTCTGCCATG ATGATTGCAT TGGCCCCTCC AAACTCTGTG TCTCCTGCCT TGTTGTTCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_006515241.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515304.1 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X7, mRNA.

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CloneID	OMu47431
Clone ID Related Accession (Same CDS sequence)	XM_006515241.2
Accession Version	XM_006515241.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCT CCAATCAACA CCTCTCTCTC AAGACTGTCA AAGCAGCCAG TGACTCTGTA 
CCTGCCAAAC CTGGACAGAT GAAAAGGACT AAATGTGCTG ACATTGATGT TGAGACTCCT 
GATTCCATTC TGGTTAATAC TAATCTTCGA GCACTGATCA ACAAGCACAC CTTTTCAGTT 
CTTCCTGGAG ACTGCCAGCA GCGCCTGCTT TTGTTGCTTC CAGAGGTGGA TCGACAGGTT 
GGTCCAGATG GCCTGATGAA GTTGAATGGC TCAGCCCTTA ACAATGAGTT CTTCACTTCT 
GCAGCCCAGG GCTGGAAGGA AAGGCTCTCA GAAGGTGAAT TTACTCCTGA AATGCAGGTG 
AGAATTCGAC AAGAGATTGA GAAGGAGAAG AAAGTAGAGC TGTGGAAGGA ACAATTCTTT 
GAAAATTACT ATGGTCAGAG TTCTGGCCTA AGCCTTGAAG ATTCACAGAA ACTGACAGCT 
TCCTCAAGTG ATCCCAAAGC AAAGAAAACC CCAGCTGAGC AACCAAAATC CATACTTCCT 
TCAGAGGCCT CTCCTGTCAG AATTGTCCCA GTAGTTCCTC AGTCCGAGTG TAAAGAAGAA 
GCAGTGCAGA TACCATCACC ATCCCAAAAG GAAGAAAATC AAGACGAGGC GAGGCCAGAC 
TCCAAATCCC CAGAACCTGT TCTTGCATCA GCTAGCAATA CAAATGAGCT TATCACCATG 
AAACCCATCA AGAGCCCTAA GGATGAGGGT CTCTTAGAGC AGAAGCCTGT TGCCTGTGCT 
GAACAAGAAT CTGAGAAAGA AAATCATGTA ACCACAACTT CTCGTAATAA CAAAAGTGAG 
AACCAAGAAG CGTTAGCTAT ATCCCCAAGC AAATCTAAAA ATGCTGGGTT ACAAAAACCC 
ATAATAAAGC CTGTTGCAGA AGCCAGTCCA CTGAACCCTG ATATGAAGAT GCCACCAGCA 
ACTGTTACTG ATCAGATCCA AGAAAGTCTC AAGAGGAAAT CTTCTCTTAC TGATGAAGAG 
GCCACCAGTA GCTGGGAGAA AAGACCACGT ATCACTGAGA ATCGCCAGCA CCAGCAGCCA 
TTTCAGGTCT CCCCACAGCC TTTTCTTAAT AGAGGGGACA GGGTCCAAGT GCGAAAAGTC 
CCACCTCTCA AGATCCCTGT CTCCAGAATC TCCCCGATGC TGTTTTCTAC ATCACAGGTC 
TCTCCCAGGG CTCGTTTTCC AATCTCCATC ACTAGTCCTT ACAGAACAGG AGCCAGAACT 
CTGGCAGACA TCAAAGCAAA AGCCCAGTTG GTCAAAGCAC AAAAGGCAGC AGCCGCAGCA 
GCTGCTGCAG CTGCTGCAGC CGCCTCAGTT GGAGGGACCA TTCCGGGACC TGGCCCTGGG 
GGTGGACAAA GTCCAAGAGA GGGTGGTGAA AGGAAAATTG CTGGAGGAGG AAGTGCAGGC 
TCAGACCCAG TCAGTACAAA TGGAAAAGGT CCCACACTGG AACTGGCCGG AACTGGAAGC 
AGGGGAGGTA CGAGAGAACT TTTACCCTGT GGTCCTCAAC CTGAGACCAA TATGCCAGGC 
CAAGCACAGC CTCCTGGTAT CTCTGGAGCA CAACTACAGC AAACCTCCTC AGTGCCTACG 
GGACTTGCCA GCAGTGGAGC ATGCACAAGT GTCCCATTAC CAGCCCACAT AGAAATATCA 
AACAGTGAAA AACCAAACCT CCACAAGGCA ACAGCCACAG CAGCCTCTCC TTGCCACTTG 
CAAGACCCTA GGAGTTGCAG ACTTGAAAAA GCTCTGTCTC CAACAGGGCC TCCTCTGATC 
TCAGGAGCCT CAACTGTTTA TTTTGTAGCT GATGGCACAG TTGAGCCCAA AGCAGGTTCT 
AATAAGAATG CACCAAAGCC TTCAGCCTTA GCAAAGACAA CTGCTCCTGC TCCACTAGAT 
ATGACTTCCT CTCCTGTGAC AACAGCCAGT TTAGAAAAAC TCCCTGTACC CCAGATCAGT 
GGAACTGCAA CATCTACTGG ATCAGCTCCA TCCTCAAGCA CTTTGCCAGC AGCTTCTAGC 
CTTAAAACCC CAGGAACTTC TGCAAATATG AATGGACCCA TTTCAAGAAC AAGTTCTAGT 
ATCCCTGCTA ATAATCCTTT AGTTACTCAG CTGCTCCAGG GCAAAGATGT TCCCTTGGAG 
CAAATTCTGC CTAAGCCTCT CACCAAAATT GAAATGAAAA CAGTTCCACT GACTACAAAA 
GAGGAAAAAG GCATAGGGAT ATTCCCTGGT ATCAGTGTAA TGGAAAGTAG CAGCAGAGAG 
GAAGTTAATG GCAGGCAGGC CCATCTGGCT ATCCCGCAGT TGGGGAAACC CTTGCAAAGT 
AAACAGCTTT CCCAGGTTCC AAGGCCAGTT TTCACTGCTA AGGACCGGAA GGACCCCTGC 
ATTGACACTC ACCAGTACCG AGAAGGTCTA AGTAAAACAA CGCAAGATCA GCTCTTTCAG 
ACTCTTATCC AGAGGGCTCA GAGACAGAGT GTTCTTTCAT TTGTACCGCC CTCTCAGTTC 
AACTTTGCTC ACTCAGGTTT CCACTTGGAA GACATCTCCA CAAGTCAGAA GTTCATGCTG 
GGTTTTGCTG GCAGAAGGAC ATCCAAGCCT GCAATGGCAG GTCACTATTT ACTTAATATT 
TCCACCTATG GCAGGGGTAC AGAGAACATT AAAAGGACCC ATTCTGTAAA TCCTGATGAT 
CGATTTTGTC TAAGTAGCCC CACTGAGGCG TTGAGAATGG GGCATGCAGA TTATAAAAAC 
ACAACTGGAG AAATCAGCAG CAAAGAAGAT GAAAGTGATG AAGATCGCGT AGGTGATGAA 
CAGGAGCCTA TTTCAGTGAA GGAGGAGCCA TGGGCTTCCC AGAGTTCTGG CAGGCATCCG 
CACCATGGGG AAGCTTCATC TACCAATGAT TGTTTAGCTA GCAAGAATGG TAAGACTGAG 
GCACCAGTGA GTGAGCAAAC CACTTTAGGC CAGGAGAATT ACATATTCTC TAGAGGCCAA 
GCATCTGATG AAAAGAGCCT GCCTAGAGAT TTTATTCCGG CAGCACATAA ACAGATGACT 
CATGCTGTGA GAGGTAAGAC AGTGTGTAGC AGCCCTGAGC TTTTCAATTC TACTGCTCTT 
TCTCTTCCCG CAGACAGTCC CACACATCAG CCTCTACTCC TTCCACCACT GCAAACCCCC 
AAATTGTATG GAAGCCCCAC ACAGATAGGG CCAAGCTATA GAGGCATGAT CAATGTCTCC 
ACCTCATCAG ACATGGACCA TAACTCTGCT ATACCAGGCA GCCAGGTATC TAGCAATGTA 
GGGGACGTCA TGTCGTTTTC AGTGACTGTC ACTACCATCC CTGCCAGTCA AGCAATGAAT 
CCCAGCAGCC ATGGCCAGAC AATTCCTGTT CAGACTTTTC CTGATGACAA TAGTATCGAG 
GACACACCAT CAAAATGTTA CTGCCGGTTG AAAGCCATGA TCATGTGCAA AGGATGTGGA 
GCTTTCTGCC ATGATGATTG CATTGGCCCC TCCAAACTCT GTGTCTCCTG CCTTGTTGTT 
CGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515304.1
CDS	573..4118
Translation	MSISNQHLSLKTVKAASDSVPAKPGQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_006515241.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515241.2

ORF Insert Sequence:

ATGTCCATCT CCAATCAACA CCTCTCTCTC AAGACTGTCA AAGCAGCCAG TGACTCTGTA 
CCTGCCAAAC CTGGACAGAT GAAAAGGACT AAATGTGCTG ACATTGATGT TGAGACTCCT 
GATTCCATTC TGGTTAATAC TAATCTTCGA GCACTGATCA ACAAGCACAC CTTTTCAGTT 
CTTCCTGGAG ACTGCCAGCA GCGCCTGCTT TTGTTGCTTC CAGAGGTGGA TCGACAGGTT 
GGTCCAGATG GCCTGATGAA GTTGAATGGC TCAGCCCTTA ACAATGAGTT CTTCACTTCT 
GCAGCCCAGG GCTGGAAGGA AAGGCTCTCA GAAGGTGAAT TTACTCCTGA AATGCAGGTG 
AGAATTCGAC AAGAGATTGA GAAGGAGAAG AAAGTAGAGC TGTGGAAGGA ACAATTCTTT 
GAAAATTACT ATGGTCAGAG TTCTGGCCTA AGCCTTGAAG ATTCACAGAA ACTGACAGCT 
TCCTCAAGTG ATCCCAAAGC AAAGAAAACC CCAGCTGAGC AACCAAAATC CATACTTCCT 
TCAGAGGCCT CTCCTGTCAG AATTGTCCCA GTAGTTCCTC AGTCCGAGTG TAAAGAAGAA 
GCAGTGCAGA TACCATCACC ATCCCAAAAG GAAGAAAATC AAGACGAGGC GAGGCCAGAC 
TCCAAATCCC CAGAACCTGT TCTTGCATCA GCTAGCAATA CAAATGAGCT TATCACCATG 
AAACCCATCA AGAGCCCTAA GGATGAGGGT CTCTTAGAGC AGAAGCCTGT TGCCTGTGCT 
GAACAAGAAT CTGAGAAAGA AAATCATGTA ACCACAACTT CTCGTAATAA CAAAAGTGAG 
AACCAAGAAG CGTTAGCTAT ATCCCCAAGC AAATCTAAAA ATGCTGGGTT ACAAAAACCC 
ATAATAAAGC CTGTTGCAGA AGCCAGTCCA CTGAACCCTG ATATGAAGAT GCCACCAGCA 
ACTGTTACTG ATCAGATCCA AGAAAGTCTC AAGAGGAAAT CTTCTCTTAC TGATGAAGAG 
GCCACCAGTA GCTGGGAGAA AAGACCACGT ATCACTGAGA ATCGCCAGCA CCAGCAGCCA 
TTTCAGGTCT CCCCACAGCC TTTTCTTAAT AGAGGGGACA GGGTCCAAGT GCGAAAAGTC 
CCACCTCTCA AGATCCCTGT CTCCAGAATC TCCCCGATGC TGTTTTCTAC ATCACAGGTC 
TCTCCCAGGG CTCGTTTTCC AATCTCCATC ACTAGTCCTT ACAGAACAGG AGCCAGAACT 
CTGGCAGACA TCAAAGCAAA AGCCCAGTTG GTCAAAGCAC AAAAGGCAGC AGCCGCAGCA 
GCTGCTGCAG CTGCTGCAGC CGCCTCAGTT GGAGGGACCA TTCCGGGACC TGGCCCTGGG 
GGTGGACAAA GTCCAAGAGA GGGTGGTGAA AGGAAAATTG CTGGAGGAGG AAGTGCAGGC 
TCAGACCCAG TCAGTACAAA TGGAAAAGGT CCCACACTGG AACTGGCCGG AACTGGAAGC 
AGGGGAGGTA CGAGAGAACT TTTACCCTGT GGTCCTCAAC CTGAGACCAA TATGCCAGGC 
CAAGCACAGC CTCCTGGTAT CTCTGGAGCA CAACTACAGC AAACCTCCTC AGTGCCTACG 
GGACTTGCCA GCAGTGGAGC ATGCACAAGT GTCCCATTAC CAGCCCACAT AGAAATATCA 
AACAGTGAAA AACCAAACCT CCACAAGGCA ACAGCCACAG CAGCCTCTCC TTGCCACTTG 
CAAGACCCTA GGAGTTGCAG ACTTGAAAAA GCTCTGTCTC CAACAGGGCC TCCTCTGATC 
TCAGGAGCCT CAACTGTTTA TTTTGTAGCT GATGGCACAG TTGAGCCCAA AGCAGGTTCT 
AATAAGAATG CACCAAAGCC TTCAGCCTTA GCAAAGACAA CTGCTCCTGC TCCACTAGAT 
ATGACTTCCT CTCCTGTGAC AACAGCCAGT TTAGAAAAAC TCCCTGTACC CCAGATCAGT 
GGAACTGCAA CATCTACTGG ATCAGCTCCA TCCTCAAGCA CTTTGCCAGC AGCTTCTAGC 
CTTAAAACCC CAGGAACTTC TGCAAATATG AATGGACCCA TTTCAAGAAC AAGTTCTAGT 
ATCCCTGCTA ATAATCCTTT AGTTACTCAG CTGCTCCAGG GCAAAGATGT TCCCTTGGAG 
CAAATTCTGC CTAAGCCTCT CACCAAAATT GAAATGAAAA CAGTTCCACT GACTACAAAA 
GAGGAAAAAG GCATAGGGAT ATTCCCTGGT ATCAGTGTAA TGGAAAGTAG CAGCAGAGAG 
GAAGTTAATG GCAGGCAGGC CCATCTGGCT ATCCCGCAGT TGGGGAAACC CTTGCAAAGT 
AAACAGCTTT CCCAGGTTCC AAGGCCAGTT TTCACTGCTA AGGACCGGAA GGACCCCTGC 
ATTGACACTC ACCAGTACCG AGAAGGTCTA AGTAAAACAA CGCAAGATCA GCTCTTTCAG 
ACTCTTATCC AGAGGGCTCA GAGACAGAGT GTTCTTTCAT TTGTACCGCC CTCTCAGTTC 
AACTTTGCTC ACTCAGGTTT CCACTTGGAA GACATCTCCA CAAGTCAGAA GTTCATGCTG 
GGTTTTGCTG GCAGAAGGAC ATCCAAGCCT GCAATGGCAG GTCACTATTT ACTTAATATT 
TCCACCTATG GCAGGGGTAC AGAGAACATT AAAAGGACCC ATTCTGTAAA TCCTGATGAT 
CGATTTTGTC TAAGTAGCCC CACTGAGGCG TTGAGAATGG GGCATGCAGA TTATAAAAAC 
ACAACTGGAG AAATCAGCAG CAAAGAAGAT GAAAGTGATG AAGATCGCGT AGGTGATGAA 
CAGGAGCCTA TTTCAGTGAA GGAGGAGCCA TGGGCTTCCC AGAGTTCTGG CAGGCATCCG 
CACCATGGGG AAGCTTCATC TACCAATGAT TGTTTAGCTA GCAAGAATGG TAAGACTGAG 
GCACCAGTGA GTGAGCAAAC CACTTTAGGC CAGGAGAATT ACATATTCTC TAGAGGCCAA 
GCATCTGATG AAAAGAGCCT GCCTAGAGAT TTTATTCCGG CAGCACATAA ACAGATGACT 
CATGCTGTGA GAGGTAAGAC AGTGTGTAGC AGCCCTGAGC TTTTCAATTC TACTGCTCTT 
TCTCTTCCCG CAGACAGTCC CACACATCAG CCTCTACTCC TTCCACCACT GCAAACCCCC 
AAATTGTATG GAAGCCCCAC ACAGATAGGG CCAAGCTATA GAGGCATGAT CAATGTCTCC 
ACCTCATCAG ACATGGACCA TAACTCTGCT ATACCAGGCA GCCAGGTATC TAGCAATGTA 
GGGGACGTCA TGTCGTTTTC AGTGACTGTC ACTACCATCC CTGCCAGTCA AGCAATGAAT 
CCCAGCAGCC ATGGCCAGAC AATTCCTGTT CAGACTTTTC CTGATGACAA TAGTATCGAG 
GACACACCAT CAAAATGTTA CTGCCGGTTG AAAGCCATGA TCATGTGCAA AGGATGTGGA 
GCTTTCTGCC ATGATGATTG CATTGGCCCC TCCAAACTCT GTGTCTCCTG CCTTGTTGTT 
CGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_006515242.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515305.1 Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X7, mRNA.

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CloneID	OMu47432
Clone ID Related Accession (Same CDS sequence)	XM_030246972.1 , XM_006515242.2
Accession Version	XM_006515242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515242.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAAA AGAAAGGACA GATGAAAAGG ACTAAATGTG CTGACATTGA TGTTGAGACT 
CCTGATTCCA TTCTGGTTAA TACTAATCTT CGAGCACTGA TCAACAAGCA CACCTTTTCA 
GTTCTTCCTG GAGACTGCCA GCAGCGCCTG CTTTTGTTGC TTCCAGAGGT GGATCGACAG 
GTTGGTCCAG ATGGCCTGAT GAAGTTGAAT GGCTCAGCCC TTAACAATGA GTTCTTCACT 
TCTGCAGCCC AGGGCTGGAA GGAAAGGCTC TCAGAAGGTG AATTTACTCC TGAAATGCAG 
GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCTGTGGAA GGAACAATTC 
TTTGAAAATT ACTATGGTCA GAGTTCTGGC CTAAGCCTTG AAGATTCACA GAAACTGACA 
GCTTCCTCAA GTGATCCCAA AGCAAAGAAA ACCCCAGCTG AGCAACCAAA ATCCATACTT 
CCTTCAGAGG CCTCTCCTGT CAGAATTGTC CCAGTAGTTC CTCAGTCCGA GTGTAAAGAA 
GAAGCAGTGC AGATACCATC ACCATCCCAA AAGGAAGAAA ATCAAGACGA GGCGAGGCCA 
GACTCCAAAT CCCCAGAACC TGTTCTTGCA TCAGCTAGCA ATACAAATGA GCTTATCACC 
ATGAAACCCA TCAAGAGCCC TAAGGATGAG GGTCTCTTAG AGCAGAAGCC TGTTGCCTGT 
GCTGAACAAG AATCTGAGAA AGAAAATCAT GTAACCACAA CTTCTCGTAA TAACAAAAGT 
GAGAACCAAG AAGCGTTAGC TATATCCCCA AGCAAATCTA AAAATGCTGG GTTACAAAAA 
CCCATAATAA AGCCTGTTGC AGAAGCCAGT CCACTGAACC CTGATATGAA GATGCCACCA 
GCAACTGTTA CTGATCAGAT CCAAGAAAGT CTCAAGAGGA AATCTTCTCT TACTGATGAA 
GAGGCCACCA GTAGCTGGGA GAAAAGACCA CGTATCACTG AGAATCGCCA GCACCAGCAG 
CCATTTCAGG TCTCCCCACA GCCTTTTCTT AATAGAGGGG ACAGGGTCCA AGTGCGAAAA 
GTCCCACCTC TCAAGATCCC TGTCTCCAGA ATCTCCCCGA TGCTGTTTTC TACATCACAG 
GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTTACAGAAC AGGAGCCAGA 
ACTCTGGCAG ACATCAAAGC AAAAGCCCAG TTGGTCAAAG CACAAAAGGC AGCAGCCGCA 
GCAGCTGCTG CAGCTGCTGC AGCCGCCTCA GTTGGAGGGA CCATTCCGGG ACCTGGCCCT 
GGGGGTGGAC AAAGTCCAAG AGAGGGTGGT GAAAGGAAAA TTGCTGGAGG AGGAAGTGCA 
GGCTCAGACC CAGTCAGTAC AAATGGAAAA GGTCCCACAC TGGAACTGGC CGGAACTGGA 
AGCAGGGGAG GTACGAGAGA ACTTTTACCC TGTGGTCCTC AACCTGAGAC CAATATGCCA 
GGCCAAGCAC AGCCTCCTGG TATCTCTGGA GCACAACTAC AGCAAACCTC CTCAGTGCCT 
ACGGGACTTG CCAGCAGTGG AGCATGCACA AGTGTCCCAT TACCAGCCCA CATAGAAATA 
TCAAACAGTG AAAAACCAAA CCTCCACAAG GCAACAGCCA CAGCAGCCTC TCCTTGCCAC 
TTGCAAGACC CTAGGAGTTG CAGACTTGAA AAAGCTCTGT CTCCAACAGG GCCTCCTCTG 
ATCTCAGGAG CCTCAACTGT TTATTTTGTA GCTGATGGCA CAGTTGAGCC CAAAGCAGGT 
TCTAATAAGA ATGCACCAAA GCCTTCAGCC TTAGCAAAGA CAACTGCTCC TGCTCCACTA 
GATATGACTT CCTCTCCTGT GACAACAGCC AGTTTAGAAA AACTCCCTGT ACCCCAGATC 
AGTGGAACTG CAACATCTAC TGGATCAGCT CCATCCTCAA GCACTTTGCC AGCAGCTTCT 
AGCCTTAAAA CCCCAGGAAC TTCTGCAAAT ATGAATGGAC CCATTTCAAG AACAAGTTCT 
AGTATCCCTG CTAATAATCC TTTAGTTACT CAGCTGCTCC AGGGCAAAGA TGTTCCCTTG 
GAGCAAATTC TGCCTAAGCC TCTCACCAAA ATTGAAATGA AAACAGTTCC ACTGACTACA 
AAAGAGGAAA AAGGCATAGG GATATTCCCT GGTATCAGTG TAATGGAAAG TAGCAGCAGA 
GAGGAAGTTA ATGGCAGGCA GGCCCATCTG GCTATCCCGC AGTTGGGGAA ACCCTTGCAA 
AGTAAACAGC TTTCCCAGGT TCCAAGGCCA GTTTTCACTG CTAAGGACCG GAAGGACCCC 
TGCATTGACA CTCACCAGTA CCGAGAAGGT CTAAGTAAAA CAACGCAAGA TCAGCTCTTT 
CAGACTCTTA TCCAGAGGGC TCAGAGACAG AGTGTTCTTT CATTTGTACC GCCCTCTCAG 
TTCAACTTTG CTCACTCAGG TTTCCACTTG GAAGACATCT CCACAAGTCA GAAGTTCATG 
CTGGGTTTTG CTGGCAGAAG GACATCCAAG CCTGCAATGG CAGGTCACTA TTTACTTAAT 
ATTTCCACCT ATGGCAGGGG TACAGAGAAC ATTAAAAGGA CCCATTCTGT AAATCCTGAT 
GATCGATTTT GTCTAAGTAG CCCCACTGAG GCGTTGAGAA TGGGGCATGC AGATTATAAA 
AACACAACTG GAGAAATCAG CAGCAAAGAA GATGAAAGTG ATGAAGATCG CGTAGGTGAT 
GAACAGGAGC CTATTTCAGT GAAGGAGGAG CCATGGGCTT CCCAGAGTTC TGGCAGGCAT 
CCGCACCATG GGGAAGCTTC ATCTACCAAT GATTGTTTAG CTAGCAAGAA TGGTAAGACT 
GAGGCACCAG TGAGTGAGCA AACCACTTTA GGCCAGGAGA ATTACATATT CTCTAGAGGC 
CAAGCATCTG ATGAAAAGAG CCTGCCTAGA GATTTTATTC CGGCAGCACA TAAACAGATG 
ACTCATGCTG TGAGAGGTAA GACAGTGTGT AGCAGCCCTG AGCTTTTCAA TTCTACTGCT 
CTTTCTCTTC CCGCAGACAG TCCCACACAT CAGCCTCTAC TCCTTCCACC ACTGCAAACC 
CCCAAATTGT ATGGAAGCCC CACACAGATA GGGCCAAGCT ATAGAGGCAT GATCAATGTC 
TCCACCTCAT CAGACATGGA CCATAACTCT GCTATACCAG GCAGCCAGGT ATCTAGCAAT 
GTAGGGGACG TCATGTCGTT TTCAGTGACT GTCACTACCA TCCCTGCCAG TCAAGCAATG 
AATCCCAGCA GCCATGGCCA GACAATTCCT GTTCAGACTT TTCCTGATGA CAATAGTATC 
GAGGACACAC CATCAAAATG TTACTGCCGG TTGAAAGCCA TGATCATGTG CAAAGGATGT 
GGAGCTTTCT GCCATGATGA TTGCATTGGC CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT 
GTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515305.1
CDS	2217..5705
Translation	MTEKKGQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_006515242.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515242.2

ORF Insert Sequence:

ATGACAGAAA AGAAAGGACA GATGAAAAGG ACTAAATGTG CTGACATTGA TGTTGAGACT 
CCTGATTCCA TTCTGGTTAA TACTAATCTT CGAGCACTGA TCAACAAGCA CACCTTTTCA 
GTTCTTCCTG GAGACTGCCA GCAGCGCCTG CTTTTGTTGC TTCCAGAGGT GGATCGACAG 
GTTGGTCCAG ATGGCCTGAT GAAGTTGAAT GGCTCAGCCC TTAACAATGA GTTCTTCACT 
TCTGCAGCCC AGGGCTGGAA GGAAAGGCTC TCAGAAGGTG AATTTACTCC TGAAATGCAG 
GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCTGTGGAA GGAACAATTC 
TTTGAAAATT ACTATGGTCA GAGTTCTGGC CTAAGCCTTG AAGATTCACA GAAACTGACA 
GCTTCCTCAA GTGATCCCAA AGCAAAGAAA ACCCCAGCTG AGCAACCAAA ATCCATACTT 
CCTTCAGAGG CCTCTCCTGT CAGAATTGTC CCAGTAGTTC CTCAGTCCGA GTGTAAAGAA 
GAAGCAGTGC AGATACCATC ACCATCCCAA AAGGAAGAAA ATCAAGACGA GGCGAGGCCA 
GACTCCAAAT CCCCAGAACC TGTTCTTGCA TCAGCTAGCA ATACAAATGA GCTTATCACC 
ATGAAACCCA TCAAGAGCCC TAAGGATGAG GGTCTCTTAG AGCAGAAGCC TGTTGCCTGT 
GCTGAACAAG AATCTGAGAA AGAAAATCAT GTAACCACAA CTTCTCGTAA TAACAAAAGT 
GAGAACCAAG AAGCGTTAGC TATATCCCCA AGCAAATCTA AAAATGCTGG GTTACAAAAA 
CCCATAATAA AGCCTGTTGC AGAAGCCAGT CCACTGAACC CTGATATGAA GATGCCACCA 
GCAACTGTTA CTGATCAGAT CCAAGAAAGT CTCAAGAGGA AATCTTCTCT TACTGATGAA 
GAGGCCACCA GTAGCTGGGA GAAAAGACCA CGTATCACTG AGAATCGCCA GCACCAGCAG 
CCATTTCAGG TCTCCCCACA GCCTTTTCTT AATAGAGGGG ACAGGGTCCA AGTGCGAAAA 
GTCCCACCTC TCAAGATCCC TGTCTCCAGA ATCTCCCCGA TGCTGTTTTC TACATCACAG 
GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTTACAGAAC AGGAGCCAGA 
ACTCTGGCAG ACATCAAAGC AAAAGCCCAG TTGGTCAAAG CACAAAAGGC AGCAGCCGCA 
GCAGCTGCTG CAGCTGCTGC AGCCGCCTCA GTTGGAGGGA CCATTCCGGG ACCTGGCCCT 
GGGGGTGGAC AAAGTCCAAG AGAGGGTGGT GAAAGGAAAA TTGCTGGAGG AGGAAGTGCA 
GGCTCAGACC CAGTCAGTAC AAATGGAAAA GGTCCCACAC TGGAACTGGC CGGAACTGGA 
AGCAGGGGAG GTACGAGAGA ACTTTTACCC TGTGGTCCTC AACCTGAGAC CAATATGCCA 
GGCCAAGCAC AGCCTCCTGG TATCTCTGGA GCACAACTAC AGCAAACCTC CTCAGTGCCT 
ACGGGACTTG CCAGCAGTGG AGCATGCACA AGTGTCCCAT TACCAGCCCA CATAGAAATA 
TCAAACAGTG AAAAACCAAA CCTCCACAAG GCAACAGCCA CAGCAGCCTC TCCTTGCCAC 
TTGCAAGACC CTAGGAGTTG CAGACTTGAA AAAGCTCTGT CTCCAACAGG GCCTCCTCTG 
ATCTCAGGAG CCTCAACTGT TTATTTTGTA GCTGATGGCA CAGTTGAGCC CAAAGCAGGT 
TCTAATAAGA ATGCACCAAA GCCTTCAGCC TTAGCAAAGA CAACTGCTCC TGCTCCACTA 
GATATGACTT CCTCTCCTGT GACAACAGCC AGTTTAGAAA AACTCCCTGT ACCCCAGATC 
AGTGGAACTG CAACATCTAC TGGATCAGCT CCATCCTCAA GCACTTTGCC AGCAGCTTCT 
AGCCTTAAAA CCCCAGGAAC TTCTGCAAAT ATGAATGGAC CCATTTCAAG AACAAGTTCT 
AGTATCCCTG CTAATAATCC TTTAGTTACT CAGCTGCTCC AGGGCAAAGA TGTTCCCTTG 
GAGCAAATTC TGCCTAAGCC TCTCACCAAA ATTGAAATGA AAACAGTTCC ACTGACTACA 
AAAGAGGAAA AAGGCATAGG GATATTCCCT GGTATCAGTG TAATGGAAAG TAGCAGCAGA 
GAGGAAGTTA ATGGCAGGCA GGCCCATCTG GCTATCCCGC AGTTGGGGAA ACCCTTGCAA 
AGTAAACAGC TTTCCCAGGT TCCAAGGCCA GTTTTCACTG CTAAGGACCG GAAGGACCCC 
TGCATTGACA CTCACCAGTA CCGAGAAGGT CTAAGTAAAA CAACGCAAGA TCAGCTCTTT 
CAGACTCTTA TCCAGAGGGC TCAGAGACAG AGTGTTCTTT CATTTGTACC GCCCTCTCAG 
TTCAACTTTG CTCACTCAGG TTTCCACTTG GAAGACATCT CCACAAGTCA GAAGTTCATG 
CTGGGTTTTG CTGGCAGAAG GACATCCAAG CCTGCAATGG CAGGTCACTA TTTACTTAAT 
ATTTCCACCT ATGGCAGGGG TACAGAGAAC ATTAAAAGGA CCCATTCTGT AAATCCTGAT 
GATCGATTTT GTCTAAGTAG CCCCACTGAG GCGTTGAGAA TGGGGCATGC AGATTATAAA 
AACACAACTG GAGAAATCAG CAGCAAAGAA GATGAAAGTG ATGAAGATCG CGTAGGTGAT 
GAACAGGAGC CTATTTCAGT GAAGGAGGAG CCATGGGCTT CCCAGAGTTC TGGCAGGCAT 
CCGCACCATG GGGAAGCTTC ATCTACCAAT GATTGTTTAG CTAGCAAGAA TGGTAAGACT 
GAGGCACCAG TGAGTGAGCA AACCACTTTA GGCCAGGAGA ATTACATATT CTCTAGAGGC 
CAAGCATCTG ATGAAAAGAG CCTGCCTAGA GATTTTATTC CGGCAGCACA TAAACAGATG 
ACTCATGCTG TGAGAGGTAA GACAGTGTGT AGCAGCCCTG AGCTTTTCAA TTCTACTGCT 
CTTTCTCTTC CCGCAGACAG TCCCACACAT CAGCCTCTAC TCCTTCCACC ACTGCAAACC 
CCCAAATTGT ATGGAAGCCC CACACAGATA GGGCCAAGCT ATAGAGGCAT GATCAATGTC 
TCCACCTCAT CAGACATGGA CCATAACTCT GCTATACCAG GCAGCCAGGT ATCTAGCAAT 
GTAGGGGACG TCATGTCGTT TTCAGTGACT GTCACTACCA TCCCTGCCAG TCAAGCAATG 
AATCCCAGCA GCCATGGCCA GACAATTCCT GTTCAGACTT TTCCTGATGA CAATAGTATC 
GAGGACACAC CATCAAAATG TTACTGCCGG TTGAAAGCCA TGATCATGTG CAAAGGATGT 
GGAGCTTTCT GCCATGATGA TTGCATTGGC CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT 
GTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( NM_172421.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766009.2 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant 2, mRNA.

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CloneID	OMu03419
Clone ID Related Accession (Same CDS sequence)	NM_172421.5
Accession Version	NM_172421.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK148015.1, AK036839.1, AC160138.2, AK016141.1, AC155273.3 and BP763544.1. On or before Jul 15, 2017 this sequence version replaced XM_017315230.1, XM_011243900.1, XM_006515240.1, NM_172421.4. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036839.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG AAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG GACAGATGAA AAGGACTAAA 
TGTGCTGACA TTGATGTTGA GACTCCTGAT TCCATTCTGG TTAATACTAA TCTTCGAGCA 
CTGATCAACA AGCACACCTT TTCAGTTCTT CCTGGAGACT GCCAGCAGCG CCTGCTTTTG 
TTGCTTCCAG AGGTGGATCG ACAGGTTGGT CCAGATGGCC TGATGAAGTT GAATGGCTCA 
GCCCTTAACA ATGAGTTCTT CACTTCTGCA GCCCAGGGCT GGAAGGAAAG GCTCTCAGAA 
GGTGAATTTA CTCCTGAAAT GCAGGTGAGA ATTCGACAAG AGATTGAGAA GGAGAAGAAA 
GTAGAGCTGT GGAAGGAACA ATTCTTTGAA AATTACTATG GTCAGAGTTC TGGCCTAAGC 
CTTGAAGATT CACAGAAACT GACAGCTTCC TCAAGTGATC CCAAAGCAAA GAAAACCCCA 
GCTGAGCAAC CAAAATCCAT ACTTCCTTCA GAGGCCTCTC CTGTCAGAAT TGTCCCAGTA 
GTTCCTCAGT CCGAGTGTAA AGAAGAAGCA GTGCAGATAC CATCACCATC CCAAAAGGAA 
GAAAATCAAG ACGAGGCGAG GCCAGACTCC AAATCCCCAG AACCTGTTCT TGCATCAGCT 
AGCAATACAA ATGAGCTTAT CACCATGAAA CCCATCAAGA GCCCTAAGGA TGAGGGTCTC 
TTAGAGCAGA AGCCTGTTGC CTGTGCTGAA CAAGAATCTG AGAAAGAAAA TCATGTAACC 
ACAACTTCTC GTAATAACAA AAGTGAGAAC CAAGAAGCGT TAGCTATATC CCCAAGCAAA 
TCTAAAAATG CTGGGTTACA AAAACCCATA ATAAAGCCTG TTGCAGAAGC CAGTCCACTG 
AACCCTGATA TGAAGATGCC ACCAGCAACT GTTACTGATC AGATCCAAGA AAGTCTCAAG 
AGGAAATCTT CTCTTACTGA TGAAGAGGCC ACCAGTAGCT GGGAGAAAAG ACCACGTATC 
ACTGAGAATC GCCAGCACCA GCAGCCATTT CAGGTCTCCC CACAGCCTTT TCTTAATAGA 
GGGGACAGGG TCCAAGTGCG AAAAGTCCCA CCTCTCAAGA TCCCTGTCTC CAGAATCTCC 
CCGATGCTGT TTTCTACATC ACAGGTCTCT CCCAGGGCTC GTTTTCCAAT CTCCATCACT 
AGTCCTTACA GAACAGGAGC CAGAACTCTG GCAGACATCA AAGCAAAAGC CCAGTTGGTC 
AAAGCACAAA AGGCAGCAGC CGCAGCAGCT GCTGCAGCTG CTGCAGCCGC CTCAGTTGGA 
GGGACCATTC CGGGACCTGG CCCTGGGGGT GGACAAAGTC CAAGAGAGGG TGGTGAAAGG 
AAAATTGCTG GAGGAGGAAG TGCAGGCTCA GACCCAGTCA GTACAAATGG AAAAGGTCCC 
ACACTGGAAC TGGCCGGAAC TGGAAGCAGG GGAGGTACGA GAGAACTTTT ACCCTGTGGT 
CCTCAACCTG AGACCAATAT GCCAGGCCAA GCACAGCCTC CTGGTATCTC TGGAGCACAA 
CTACAGCAAA CCTCCTCAGT GCCTACGGGA CTTGCCAGCA GTGGAGCATG CACAAGTGTC 
CCATTACCAG CCCACATAGA AATATCAAAC AGTGAAAAAC CAAACCTCCA CAAGGCAACA 
GCCACAGCAG CCTCTCCTTG CCACTTGCAA GACCCTAGGA GTTGCAGACT TGAAAAAGCT 
CTGTCTCCAA CAGGGCCTCC TCTGATCTCA GGAGCCTCAA CTGTTTATTT TGTAGCTGAT 
GGCACAGTTG AGCCCAAAGC AGGTTCTAAT AAGAATGCAC CAAAGCCTTC AGCCTTAGCA 
AAGACAACTG CTCCTGCTCC ACTAGATATG ACTTCCTCTC CTGTGACAAC AGCCAGTTTA 
GAAAAACTCC CTGTACCCCA GATCAGTGGA ACTGCAACAT CTACTGGATC AGCTCCATCC 
TCAAGCACTT TGCCAGCAGC TTCTAGCCTT AAAACCCCAG GAACTTCTGC AAATATGAAT 
GGACCCATTT CAAGAACAAG TTCTAGTATC CCTGCTAATA ATCCTTTAGT TACTCAGCTG 
CTCCAGGGCA AAGATGTTCC CTTGGAGCAA ATTCTGCCTA AGCCTCTCAC CAAAATTGAA 
ATGAAAACAG TTCCACTGAC TACAAAAGAG GAAAAAGGCA TAGGGATATT CCCTGGTATC 
AGTGTAATGG AAAGTAGCAG CAGAGAGGAA GTTAATGGCA GGCAGGCCCA TCTGGCTATC 
CCGCAGTTGG GGAAACCCTT GCAAAGTAAA CAGCTTTCCC AGGTTCCAAG GCCAGTTTTC 
ACTGCTAAGG ACCGGAAGGA CCCCTGCATT GACACTCACC AGTACCGAGA AGGTCTAAGT 
AAAACAACGC AAGATCAGCT CTTTCAGACT CTTATCCAGA GGGCTCAGAG ACAGAGTGTT 
CTTTCATTTG TACCGCCCTC TCAGTTCAAC TTTGCTCACT CAGGTTTCCA CTTGGAAGAC 
ATCTCCACAA GTCAGAAGTT CATGCTGGGT TTTGCTGGCA GAAGGACATC CAAGCCTGCA 
ATGGCAGGTC ACTATTTACT TAATATTTCC ACCTATGGCA GGGGTACAGA GAACATTAAA 
AGGACCCATT CTGTAAATCC TGATGATCGA TTTTGTCTAA GTAGCCCCAC TGAGGCGTTG 
AGAATGGGGC ATGCAGATTA TAAAAACACA ACTGGAGAAA TCAGCAGCAA AGAAGATGAA 
AGTGATGAAG ATCGCGTAGG TGATGAACAG GAGCCTATTT CAGTGAAGGA GGAGCCATGG 
GCTTCCCAGA GTTCTGGCAG GCATCCGCAC CATGGGGAAG CTTCATCTAC CAATGATTGT 
TTAGCTAGCA AGAATGGTAA GACTGAGGCA CCAGTGAGTG AGCAAACCAC TTTAGGCCAG 
GAGAATTACA TATTCTCTAG AGGCCAAGCA TCTGATGAAA AGAGCCTGCC TAGAGATTTT 
ATTCCGGCAG CACATAAACA GATGACTCAT GCTGTGAGAG GTAAGACAGT GTGTAGCAGC 
CCTGAGCTTT TCAATTCTAC TGCTCTTTCT CTTCCCGCAG ACAGTCCCAC ACATCAGCCT 
CTACTCCTTC CACCACTGCA AACCCCCAAA TTGTATGGAA GCCCCACACA GATAGGGCCA 
AGCTATAGAG GCATGATCAA TGTCTCCACC TCATCAGACA TGGACCATAA CTCTGCTATA 
CCAGGCAGCC AGGTATCTAG CAATGTAGGG GACGTCATGT CGTTTTCAGT GACTGTCACT 
ACCATCCCTG CCAGTCAAGC AATGAATCCC AGCAGCCATG GCCAGACAAT TCCTGTTCAG 
ACTTTTCCTG ATGACAATAG TATCGAGGAC ACACCATCAA AATGTTACTG CCGGTTGAAA 
GCCATGATCA TGTGCAAAGG ATGTGGAGCT TTCTGCCATG ATGATTGCAT TGGCCCCTCC 
AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766009.2
CDS	300..4412
Translation	MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEIRSGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPGQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	NM_172421.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172421.5

ORF Insert Sequence:

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG AAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG GACAGATGAA AAGGACTAAA 
TGTGCTGACA TTGATGTTGA GACTCCTGAT TCCATTCTGG TTAATACTAA TCTTCGAGCA 
CTGATCAACA AGCACACCTT TTCAGTTCTT CCTGGAGACT GCCAGCAGCG CCTGCTTTTG 
TTGCTTCCAG AGGTGGATCG ACAGGTTGGT CCAGATGGCC TGATGAAGTT GAATGGCTCA 
GCCCTTAACA ATGAGTTCTT CACTTCTGCA GCCCAGGGCT GGAAGGAAAG GCTCTCAGAA 
GGTGAATTTA CTCCTGAAAT GCAGGTGAGA ATTCGACAAG AGATTGAGAA GGAGAAGAAA 
GTAGAGCTGT GGAAGGAACA ATTCTTTGAA AATTACTATG GTCAGAGTTC TGGCCTAAGC 
CTTGAAGATT CACAGAAACT GACAGCTTCC TCAAGTGATC CCAAAGCAAA GAAAACCCCA 
GCTGAGCAAC CAAAATCCAT ACTTCCTTCA GAGGCCTCTC CTGTCAGAAT TGTCCCAGTA 
GTTCCTCAGT CCGAGTGTAA AGAAGAAGCA GTGCAGATAC CATCACCATC CCAAAAGGAA 
GAAAATCAAG ACGAGGCGAG GCCAGACTCC AAATCCCCAG AACCTGTTCT TGCATCAGCT 
AGCAATACAA ATGAGCTTAT CACCATGAAA CCCATCAAGA GCCCTAAGGA TGAGGGTCTC 
TTAGAGCAGA AGCCTGTTGC CTGTGCTGAA CAAGAATCTG AGAAAGAAAA TCATGTAACC 
ACAACTTCTC GTAATAACAA AAGTGAGAAC CAAGAAGCGT TAGCTATATC CCCAAGCAAA 
TCTAAAAATG CTGGGTTACA AAAACCCATA ATAAAGCCTG TTGCAGAAGC CAGTCCACTG 
AACCCTGATA TGAAGATGCC ACCAGCAACT GTTACTGATC AGATCCAAGA AAGTCTCAAG 
AGGAAATCTT CTCTTACTGA TGAAGAGGCC ACCAGTAGCT GGGAGAAAAG ACCACGTATC 
ACTGAGAATC GCCAGCACCA GCAGCCATTT CAGGTCTCCC CACAGCCTTT TCTTAATAGA 
GGGGACAGGG TCCAAGTGCG AAAAGTCCCA CCTCTCAAGA TCCCTGTCTC CAGAATCTCC 
CCGATGCTGT TTTCTACATC ACAGGTCTCT CCCAGGGCTC GTTTTCCAAT CTCCATCACT 
AGTCCTTACA GAACAGGAGC CAGAACTCTG GCAGACATCA AAGCAAAAGC CCAGTTGGTC 
AAAGCACAAA AGGCAGCAGC CGCAGCAGCT GCTGCAGCTG CTGCAGCCGC CTCAGTTGGA 
GGGACCATTC CGGGACCTGG CCCTGGGGGT GGACAAAGTC CAAGAGAGGG TGGTGAAAGG 
AAAATTGCTG GAGGAGGAAG TGCAGGCTCA GACCCAGTCA GTACAAATGG AAAAGGTCCC 
ACACTGGAAC TGGCCGGAAC TGGAAGCAGG GGAGGTACGA GAGAACTTTT ACCCTGTGGT 
CCTCAACCTG AGACCAATAT GCCAGGCCAA GCACAGCCTC CTGGTATCTC TGGAGCACAA 
CTACAGCAAA CCTCCTCAGT GCCTACGGGA CTTGCCAGCA GTGGAGCATG CACAAGTGTC 
CCATTACCAG CCCACATAGA AATATCAAAC AGTGAAAAAC CAAACCTCCA CAAGGCAACA 
GCCACAGCAG CCTCTCCTTG CCACTTGCAA GACCCTAGGA GTTGCAGACT TGAAAAAGCT 
CTGTCTCCAA CAGGGCCTCC TCTGATCTCA GGAGCCTCAA CTGTTTATTT TGTAGCTGAT 
GGCACAGTTG AGCCCAAAGC AGGTTCTAAT AAGAATGCAC CAAAGCCTTC AGCCTTAGCA 
AAGACAACTG CTCCTGCTCC ACTAGATATG ACTTCCTCTC CTGTGACAAC AGCCAGTTTA 
GAAAAACTCC CTGTACCCCA GATCAGTGGA ACTGCAACAT CTACTGGATC AGCTCCATCC 
TCAAGCACTT TGCCAGCAGC TTCTAGCCTT AAAACCCCAG GAACTTCTGC AAATATGAAT 
GGACCCATTT CAAGAACAAG TTCTAGTATC CCTGCTAATA ATCCTTTAGT TACTCAGCTG 
CTCCAGGGCA AAGATGTTCC CTTGGAGCAA ATTCTGCCTA AGCCTCTCAC CAAAATTGAA 
ATGAAAACAG TTCCACTGAC TACAAAAGAG GAAAAAGGCA TAGGGATATT CCCTGGTATC 
AGTGTAATGG AAAGTAGCAG CAGAGAGGAA GTTAATGGCA GGCAGGCCCA TCTGGCTATC 
CCGCAGTTGG GGAAACCCTT GCAAAGTAAA CAGCTTTCCC AGGTTCCAAG GCCAGTTTTC 
ACTGCTAAGG ACCGGAAGGA CCCCTGCATT GACACTCACC AGTACCGAGA AGGTCTAAGT 
AAAACAACGC AAGATCAGCT CTTTCAGACT CTTATCCAGA GGGCTCAGAG ACAGAGTGTT 
CTTTCATTTG TACCGCCCTC TCAGTTCAAC TTTGCTCACT CAGGTTTCCA CTTGGAAGAC 
ATCTCCACAA GTCAGAAGTT CATGCTGGGT TTTGCTGGCA GAAGGACATC CAAGCCTGCA 
ATGGCAGGTC ACTATTTACT TAATATTTCC ACCTATGGCA GGGGTACAGA GAACATTAAA 
AGGACCCATT CTGTAAATCC TGATGATCGA TTTTGTCTAA GTAGCCCCAC TGAGGCGTTG 
AGAATGGGGC ATGCAGATTA TAAAAACACA ACTGGAGAAA TCAGCAGCAA AGAAGATGAA 
AGTGATGAAG ATCGCGTAGG TGATGAACAG GAGCCTATTT CAGTGAAGGA GGAGCCATGG 
GCTTCCCAGA GTTCTGGCAG GCATCCGCAC CATGGGGAAG CTTCATCTAC CAATGATTGT 
TTAGCTAGCA AGAATGGTAA GACTGAGGCA CCAGTGAGTG AGCAAACCAC TTTAGGCCAG 
GAGAATTACA TATTCTCTAG AGGCCAAGCA TCTGATGAAA AGAGCCTGCC TAGAGATTTT 
ATTCCGGCAG CACATAAACA GATGACTCAT GCTGTGAGAG GTAAGACAGT GTGTAGCAGC 
CCTGAGCTTT TCAATTCTAC TGCTCTTTCT CTTCCCGCAG ACAGTCCCAC ACATCAGCCT 
CTACTCCTTC CACCACTGCA AACCCCCAAA TTGTATGGAA GCCCCACACA GATAGGGCCA 
AGCTATAGAG GCATGATCAA TGTCTCCACC TCATCAGACA TGGACCATAA CTCTGCTATA 
CCAGGCAGCC AGGTATCTAG CAATGTAGGG GACGTCATGT CGTTTTCAGT GACTGTCACT 
ACCATCCCTG CCAGTCAAGC AATGAATCCC AGCAGCCATG GCCAGACAAT TCCTGTTCAG 
ACTTTTCCTG ATGACAATAG TATCGAGGAC ACACCATCAA AATGTTACTG CCGGTTGAAA 
GCCATGATCA TGTGCAAAGG ATGTGGAGCT TTCTGCCATG ATGATTGCAT TGGCCCCTCC 
AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( NM_001270988.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001257917.1 Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant 1, mRNA.

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CloneID	OMu06891
Clone ID Related Accession (Same CDS sequence)	NM_001270988.1
Accession Version	NM_001270988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK148015.1, AK080679.1, AC160138.2, AK036839.1, AC155273.3 and BP763544.1. On or before Jul 15, 2017 this sequence version replaced XM_017315229.1, XM_011243899.1, XM_006515238.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC141140.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG AAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG CCCTATGGGA AGGAAAGCAA 
TCTGATGGAC AGTCAAGCAG TCCCCAGAAC TCAAATTCCA GCTTTTCTTC CTCAGTGAAA 
GTGGAAAGTT CTCTGCTAGG CTTGGGGAAG AAGTCTTTCC AGAGGTCTGA CAGACTCCAC 
ACAAGACAGA TGAAAAGGAC TAAATGTGCT GACATTGATG TTGAGACTCC TGATTCCATT 
CTGGTTAATA CTAATCTTCG AGCACTGATC AACAAGCACA CCTTTTCAGT TCTTCCTGGA 
GACTGCCAGC AGCGCCTGCT TTTGTTGCTT CCAGAGGTGG ATCGACAGGT TGGTCCAGAT 
GGCCTGATGA AGTTGAATGG CTCAGCCCTT AACAATGAGT TCTTCACTTC TGCAGCCCAG 
GGCTGGAAGG AAAGGCTCTC AGAAGGTGAA TTTACTCCTG AAATGCAGGT GAGAATTCGA 
CAAGAGATTG AGAAGGAGAA GAAAGTAGAG CTGTGGAAGG AACAATTCTT TGAAAATTAC 
TATGGTCAGA GTTCTGGCCT AAGCCTTGAA GATTCACAGA AACTGACAGC TTCCTCAAGT 
GATCCCAAAG CAAAGAAAAC CCCAGCTGAG CAACCAAAAT CCATACTTCC TTCAGAGGCC 
TCTCCTGTCA GAATTGTCCC AGTAGTTCCT CAGTCCGAGT GTAAAGAAGA AGCAGTGCAG 
ATACCATCAC CATCCCAAAA GGAAGAAAAT CAAGACGAGG CGAGGCCAGA CTCCAAATCC 
CCAGAACCTG TTCTTGCATC AGCTAGCAAT ACAAATGAGC TTATCACCAT GAAACCCATC 
AAGAGCCCTA AGGATGAGGG TCTCTTAGAG CAGAAGCCTG TTGCCTGTGC TGAACAAGAA 
TCTGAGAAAG AAAATCATGT AACCACAACT TCTCGTAATA ACAAAAGTGA GAACCAAGAA 
GCGTTAGCTA TATCCCCAAG CAAATCTAAA AATGCTGGGT TACAAAAACC CATAATAAAG 
CCTGTTGCAG AAGCCAGTCC ACTGAACCCT GATATGAAGA TGCCACCAGC AACTGTTACT 
GATCAGATCC AAGAAAGTCT CAAGAGGAAA TCTTCTCTTA CTGATGAAGA GGCCACCAGT 
AGCTGGGAGA AAAGACCACG TATCACTGAG AATCGCCAGC ACCAGCAGCC ATTTCAGGTC 
TCCCCACAGC CTTTTCTTAA TAGAGGGGAC AGGGTCCAAG TGCGAAAAGT CCCACCTCTC 
AAGATCCCTG TCTCCAGAAT CTCCCCGATG CTGTTTTCTA CATCACAGGT CTCTCCCAGG 
GCTCGTTTTC CAATCTCCAT CACTAGTCCT TACAGAACAG GAGCCAGAAC TCTGGCAGAC 
ATCAAAGCAA AAGCCCAGTT GGTCAAAGCA CAAAAGGCAG CAGCCGCAGC AGCTGCTGCA 
GCTGCTGCAG CCGCCTCAGT TGGAGGGACC ATTCCGGGAC CTGGCCCTGG GGGTGGACAA 
AGTCCAAGAG AGGGTGGTGA AAGGAAAATT GCTGGAGGAG GAAGTGCAGG CTCAGACCCA 
GTCAGTACAA ATGGAAAAGG TCCCACACTG GAACTGGCCG GAACTGGAAG CAGGGGAGGT 
ACGAGAGAAC TTTTACCCTG TGGTCCTCAA CCTGAGACCA ATATGCCAGG CCAAGCACAG 
CCTCCTGGTA TCTCTGGAGC ACAACTACAG CAAACCTCCT CAGTGCCTAC GGGACTTGCC 
AGCAGTGGAG CATGCACAAG TGTCCCATTA CCAGCCCACA TAGAAATATC AAACAGTGAA 
AAACCAAACC TCCACAAGGC AACAGCCACA GCAGCCTCTC CTTGCCACTT GCAAGACCCT 
AGGAGTTGCA GACTTGAAAA AGCTCTGTCT CCAACAGGGC CTCCTCTGAT CTCAGGAGCC 
TCAACTGTTT ATTTTGTAGC TGATGGCACA GTTGAGCCCA AAGCAGGTTC TAATAAGAAT 
GCACCAAAGC CTTCAGCCTT AGCAAAGACA ACTGCTCCTG CTCCACTAGA TATGACTTCC 
TCTCCTGTGA CAACAGCCAG TTTAGAAAAA CTCCCTGTAC CCCAGATCAG TGGAACTGCA 
ACATCTACTG GATCAGCTCC ATCCTCAAGC ACTTTGCCAG CAGCTTCTAG CCTTAAAACC 
CCAGGAACTT CTGCAAATAT GAATGGACCC ATTTCAAGAA CAAGTTCTAG TATCCCTGCT 
AATAATCCTT TAGTTACTCA GCTGCTCCAG GGCAAAGATG TTCCCTTGGA GCAAATTCTG 
CCTAAGCCTC TCACCAAAAT TGAAATGAAA ACAGTTCCAC TGACTACAAA AGAGGAAAAA 
GGCATAGGGA TATTCCCTGG TATCAGTGTA ATGGAAAGTA GCAGCAGAGA GGAAGTTAAT 
GGCAGGCAGG CCCATCTGGC TATCCCGCAG TTGGGGAAAC CCTTGCAAAG TAAACAGCTT 
TCCCAGGTTC CAAGGCCAGT TTTCACTGCT AAGGACCGGA AGGACCCCTG CATTGACACT 
CACCAGTACC GAGAAGGTCT AAGTAAAACA ACGCAAGATC AGCTCTTTCA GACTCTTATC 
CAGAGGGCTC AGAGACAGAG TGTTCTTTCA TTTGTACCGC CCTCTCAGTT CAACTTTGCT 
CACTCAGGTT TCCACTTGGA AGACATCTCC ACAAGTCAGA AGTTCATGCT GGGTTTTGCT 
GGCAGAAGGA CATCCAAGCC TGCAATGGCA GGTCACTATT TACTTAATAT TTCCACCTAT 
GGCAGGGGTA CAGAGAACAT TAAAAGGACC CATTCTGTAA ATCCTGATGA TCGATTTTGT 
CTAAGTAGCC CCACTGAGGC GTTGAGAATG GGGCATGCAG ATTATAAAAA CACAACTGGA 
GAAATCAGCA GCAAAGAAGA TGAAAGTGAT GAAGATCGCG TAGGTGATGA ACAGGAGCCT 
ATTTCAGTGA AGGAGGAGCC ATGGGCTTCC CAGAGTTCTG GCAGGCATCC GCACCATGGG 
GAAGCTTCAT CTACCAATGA TTGTTTAGCT AGCAAGAATG GTAAGACTGA GGCACCAGTG 
AGTGAGCAAA CCACTTTAGG CCAGGAGAAT TACATATTCT CTAGAGGCCA AGCATCTGAT 
GAAAAGAGCC TGCCTAGAGA TTTTATTCCG GCAGCACATA AACAGATGAC TCATGCTGTG 
AGAGGTAAGA CAGTGTGTAG CAGCCCTGAG CTTTTCAATT CTACTGCTCT TTCTCTTCCC 
GCAGACAGTC CCACACATCA GCCTCTACTC CTTCCACCAC TGCAAACCCC CAAATTGTAT 
GGAAGCCCCA CACAGATAGG GCCAAGCTAT AGAGGCATGA TCAATGTCTC CACCTCATCA 
GACATGGACC ATAACTCTGC TATACCAGGC AGCCAGGTAT CTAGCAATGT AGGGGACGTC 
ATGTCGTTTT CAGTGACTGT CACTACCATC CCTGCCAGTC AAGCAATGAA TCCCAGCAGC 
CATGGCCAGA CAATTCCTGT TCAGACTTTT CCTGATGACA ATAGTATCGA GGACACACCA 
TCAAAATGTT ACTGCCGGTT GAAAGCCATG ATCATGTGCA AAGGATGTGG AGCTTTCTGC 
CATGATGATT GCATTGGCCC CTCCAAACTC TGTGTCTCCT GCCTTGTTGT TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257917.1
CDS	300..4556
Translation	MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEIRSGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	NM_001270988.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2, transcriptional regulator (Asxl2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270988.1

ORF Insert Sequence:

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG AAGTGGGACT TCCCCTCTTG CATGCTTGAA TGCAATGCTG 
CACACAAACT CCCGAGGAGA AGAAGGCATC TTCTATAAAG TTCCAGGTAG AATGGGCGTG 
TATACTTTGA AGAAAGATGT GCCAGATGGA GTGAAAGAGC TGTCAGAAGG TTCAGAAGAA 
AGCAGTGATG GTCAGTCAGA TTCTCAGAGC TCTGAGAACA GCAGCAGCAG TGATGGTGGC 
AGCAACAAAG AAGGGAGAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG ACTGTCACAT 
CCACCCTCTC CCCCGTCAGG CTGCCCATCA CCCACCATTC CAGCAAGTAA AGTCATTTCT 
CCATCACAGA AGCACAGCAA AAAGGCATTA AAACAGGCCT TGAAGCAGCA ACAACAGAAG 
AAGAAGCAGC AGCAATGCAG ACCAAGCATG TCCATCTCCA ATCAACACCT CTCTCTCAAG 
ACTGTCAAAG CAGCCAGTGA CTCTGTACCT GCCAAACCTG CCCTATGGGA AGGAAAGCAA 
TCTGATGGAC AGTCAAGCAG TCCCCAGAAC TCAAATTCCA GCTTTTCTTC CTCAGTGAAA 
GTGGAAAGTT CTCTGCTAGG CTTGGGGAAG AAGTCTTTCC AGAGGTCTGA CAGACTCCAC 
ACAAGACAGA TGAAAAGGAC TAAATGTGCT GACATTGATG TTGAGACTCC TGATTCCATT 
CTGGTTAATA CTAATCTTCG AGCACTGATC AACAAGCACA CCTTTTCAGT TCTTCCTGGA 
GACTGCCAGC AGCGCCTGCT TTTGTTGCTT CCAGAGGTGG ATCGACAGGT TGGTCCAGAT 
GGCCTGATGA AGTTGAATGG CTCAGCCCTT AACAATGAGT TCTTCACTTC TGCAGCCCAG 
GGCTGGAAGG AAAGGCTCTC AGAAGGTGAA TTTACTCCTG AAATGCAGGT GAGAATTCGA 
CAAGAGATTG AGAAGGAGAA GAAAGTAGAG CTGTGGAAGG AACAATTCTT TGAAAATTAC 
TATGGTCAGA GTTCTGGCCT AAGCCTTGAA GATTCACAGA AACTGACAGC TTCCTCAAGT 
GATCCCAAAG CAAAGAAAAC CCCAGCTGAG CAACCAAAAT CCATACTTCC TTCAGAGGCC 
TCTCCTGTCA GAATTGTCCC AGTAGTTCCT CAGTCCGAGT GTAAAGAAGA AGCAGTGCAG 
ATACCATCAC CATCCCAAAA GGAAGAAAAT CAAGACGAGG CGAGGCCAGA CTCCAAATCC 
CCAGAACCTG TTCTTGCATC AGCTAGCAAT ACAAATGAGC TTATCACCAT GAAACCCATC 
AAGAGCCCTA AGGATGAGGG TCTCTTAGAG CAGAAGCCTG TTGCCTGTGC TGAACAAGAA 
TCTGAGAAAG AAAATCATGT AACCACAACT TCTCGTAATA ACAAAAGTGA GAACCAAGAA 
GCGTTAGCTA TATCCCCAAG CAAATCTAAA AATGCTGGGT TACAAAAACC CATAATAAAG 
CCTGTTGCAG AAGCCAGTCC ACTGAACCCT GATATGAAGA TGCCACCAGC AACTGTTACT 
GATCAGATCC AAGAAAGTCT CAAGAGGAAA TCTTCTCTTA CTGATGAAGA GGCCACCAGT 
AGCTGGGAGA AAAGACCACG TATCACTGAG AATCGCCAGC ACCAGCAGCC ATTTCAGGTC 
TCCCCACAGC CTTTTCTTAA TAGAGGGGAC AGGGTCCAAG TGCGAAAAGT CCCACCTCTC 
AAGATCCCTG TCTCCAGAAT CTCCCCGATG CTGTTTTCTA CATCACAGGT CTCTCCCAGG 
GCTCGTTTTC CAATCTCCAT CACTAGTCCT TACAGAACAG GAGCCAGAAC TCTGGCAGAC 
ATCAAAGCAA AAGCCCAGTT GGTCAAAGCA CAAAAGGCAG CAGCCGCAGC AGCTGCTGCA 
GCTGCTGCAG CCGCCTCAGT TGGAGGGACC ATTCCGGGAC CTGGCCCTGG GGGTGGACAA 
AGTCCAAGAG AGGGTGGTGA AAGGAAAATT GCTGGAGGAG GAAGTGCAGG CTCAGACCCA 
GTCAGTACAA ATGGAAAAGG TCCCACACTG GAACTGGCCG GAACTGGAAG CAGGGGAGGT 
ACGAGAGAAC TTTTACCCTG TGGTCCTCAA CCTGAGACCA ATATGCCAGG CCAAGCACAG 
CCTCCTGGTA TCTCTGGAGC ACAACTACAG CAAACCTCCT CAGTGCCTAC GGGACTTGCC 
AGCAGTGGAG CATGCACAAG TGTCCCATTA CCAGCCCACA TAGAAATATC AAACAGTGAA 
AAACCAAACC TCCACAAGGC AACAGCCACA GCAGCCTCTC CTTGCCACTT GCAAGACCCT 
AGGAGTTGCA GACTTGAAAA AGCTCTGTCT CCAACAGGGC CTCCTCTGAT CTCAGGAGCC 
TCAACTGTTT ATTTTGTAGC TGATGGCACA GTTGAGCCCA AAGCAGGTTC TAATAAGAAT 
GCACCAAAGC CTTCAGCCTT AGCAAAGACA ACTGCTCCTG CTCCACTAGA TATGACTTCC 
TCTCCTGTGA CAACAGCCAG TTTAGAAAAA CTCCCTGTAC CCCAGATCAG TGGAACTGCA 
ACATCTACTG GATCAGCTCC ATCCTCAAGC ACTTTGCCAG CAGCTTCTAG CCTTAAAACC 
CCAGGAACTT CTGCAAATAT GAATGGACCC ATTTCAAGAA CAAGTTCTAG TATCCCTGCT 
AATAATCCTT TAGTTACTCA GCTGCTCCAG GGCAAAGATG TTCCCTTGGA GCAAATTCTG 
CCTAAGCCTC TCACCAAAAT TGAAATGAAA ACAGTTCCAC TGACTACAAA AGAGGAAAAA 
GGCATAGGGA TATTCCCTGG TATCAGTGTA ATGGAAAGTA GCAGCAGAGA GGAAGTTAAT 
GGCAGGCAGG CCCATCTGGC TATCCCGCAG TTGGGGAAAC CCTTGCAAAG TAAACAGCTT 
TCCCAGGTTC CAAGGCCAGT TTTCACTGCT AAGGACCGGA AGGACCCCTG CATTGACACT 
CACCAGTACC GAGAAGGTCT AAGTAAAACA ACGCAAGATC AGCTCTTTCA GACTCTTATC 
CAGAGGGCTC AGAGACAGAG TGTTCTTTCA TTTGTACCGC CCTCTCAGTT CAACTTTGCT 
CACTCAGGTT TCCACTTGGA AGACATCTCC ACAAGTCAGA AGTTCATGCT GGGTTTTGCT 
GGCAGAAGGA CATCCAAGCC TGCAATGGCA GGTCACTATT TACTTAATAT TTCCACCTAT 
GGCAGGGGTA CAGAGAACAT TAAAAGGACC CATTCTGTAA ATCCTGATGA TCGATTTTGT 
CTAAGTAGCC CCACTGAGGC GTTGAGAATG GGGCATGCAG ATTATAAAAA CACAACTGGA 
GAAATCAGCA GCAAAGAAGA TGAAAGTGAT GAAGATCGCG TAGGTGATGA ACAGGAGCCT 
ATTTCAGTGA AGGAGGAGCC ATGGGCTTCC CAGAGTTCTG GCAGGCATCC GCACCATGGG 
GAAGCTTCAT CTACCAATGA TTGTTTAGCT AGCAAGAATG GTAAGACTGA GGCACCAGTG 
AGTGAGCAAA CCACTTTAGG CCAGGAGAAT TACATATTCT CTAGAGGCCA AGCATCTGAT 
GAAAAGAGCC TGCCTAGAGA TTTTATTCCG GCAGCACATA AACAGATGAC TCATGCTGTG 
AGAGGTAAGA CAGTGTGTAG CAGCCCTGAG CTTTTCAATT CTACTGCTCT TTCTCTTCCC 
GCAGACAGTC CCACACATCA GCCTCTACTC CTTCCACCAC TGCAAACCCC CAAATTGTAT 
GGAAGCCCCA CACAGATAGG GCCAAGCTAT AGAGGCATGA TCAATGTCTC CACCTCATCA 
GACATGGACC ATAACTCTGC TATACCAGGC AGCCAGGTAT CTAGCAATGT AGGGGACGTC 
ATGTCGTTTT CAGTGACTGT CACTACCATC CCTGCCAGTC AAGCAATGAA TCCCAGCAGC 
CATGGCCAGA CAATTCCTGT TCAGACTTTT CCTGATGACA ATAGTATCGA GGACACACCA 
TCAAAATGTT ACTGCCGGTT GAAAGCCATG ATCATGTGCA AAGGATGTGG AGCTTTCTGC 
CATGATGATT GCATTGGCCC CTCCAAACTC TGTGTCTCCT GCCTTGTTGT TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asxl2 ( XM_006515239.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515302.1 Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X2, mRNA.

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CloneID	OMu47430
Clone ID Related Accession (Same CDS sequence)	XM_006515239.4 , XM_006515239.3
Accession Version	XM_006515239.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative Polycomb group protein ASXL2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515239.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TGGGACTTCC CCTCTTGCAT GCTTGAATGC AATGCTGCAC 
ACAAACTCCC GAGGAGAAGA AGGCATCTTC TATAAAGTTC CAGGTAGAAT GGGCGTGTAT 
ACTTTGAAGA AAGATGTGCC AGATGGAGTG AAAGAGCTGT CAGAAGGTTC AGAAGAAAGC 
AGTGATGGTC AGTCAGATTC TCAGAGCTCT GAGAACAGCA GCAGCAGTGA TGGTGGCAGC 
AACAAAGAAG GGAGAAAGAG CAGGTGGAAA AGGAAAGTAT CATCTAGACT GTCACATCCA 
CCCTCTCCCC CGTCAGGCTG CCCATCACCC ACCATTCCAG CAAGTAAAGT CATTTCTCCA 
TCACAGAAGC ACAGCAAAAA GGCATTAAAA CAGGCCTTGA AGCAGCAACA ACAGAAGAAG 
AAGCAGCAGC AATGCAGACC AAGCATGTCC ATCTCCAATC AACACCTCTC TCTCAAGACT 
GTCAAAGCAG CCAGTGACTC TGTACCTGCC AAACCTGCCC TATGGGAAGG AAAGCAATCT 
GATGGACAGT CAAGCAGTCC CCAGAACTCA AATTCCAGCT TTTCTTCCTC AGTGAAAGTG 
GAAAGTTCTC TGCTAGGCTT GGGGAAGAAG TCTTTCCAGA GGTCTGACAG ACTCCACACA 
AGACAGATGA AAAGGACTAA ATGTGCTGAC ATTGATGTTG AGACTCCTGA TTCCATTCTG 
GTTAATACTA ATCTTCGAGC ACTGATCAAC AAGCACACCT TTTCAGTTCT TCCTGGAGAC 
TGCCAGCAGC GCCTGCTTTT GTTGCTTCCA GAGGTGGATC GACAGGTTGG TCCAGATGGC 
CTGATGAAGT TGAATGGCTC AGCCCTTAAC AATGAGTTCT TCACTTCTGC AGCCCAGGGC 
TGGAAGGAAA GGCTCTCAGA AGGTGAATTT ACTCCTGAAA TGCAGGTGAG AATTCGACAA 
GAGATTGAGA AGGAGAAGAA AGTAGAGCTG TGGAAGGAAC AATTCTTTGA AAATTACTAT 
GGTCAGAGTT CTGGCCTAAG CCTTGAAGAT TCACAGAAAC TGACAGCTTC CTCAAGTGAT 
CCCAAAGCAA AGAAAACCCC AGCTGAGCAA CCAAAATCCA TACTTCCTTC AGAGGCCTCT 
CCTGTCAGAA TTGTCCCAGT AGTTCCTCAG TCCGAGTGTA AAGAAGAAGC AGTGCAGATA 
CCATCACCAT CCCAAAAGGA AGAAAATCAA GACGAGGCGA GGCCAGACTC CAAATCCCCA 
GAACCTGTTC TTGCATCAGC TAGCAATACA AATGAGCTTA TCACCATGAA ACCCATCAAG 
AGCCCTAAGG ATGAGGGTCT CTTAGAGCAG AAGCCTGTTG CCTGTGCTGA ACAAGAATCT 
GAGAAAGAAA ATCATGTAAC CACAACTTCT CGTAATAACA AAAGTGAGAA CCAAGAAGCG 
TTAGCTATAT CCCCAAGCAA ATCTAAAAAT GCTGGGTTAC AAAAACCCAT AATAAAGCCT 
GTTGCAGAAG CCAGTCCACT GAACCCTGAT ATGAAGATGC CACCAGCAAC TGTTACTGAT 
CAGATCCAAG AAAGTCTCAA GAGGAAATCT TCTCTTACTG ATGAAGAGGC CACCAGTAGC 
TGGGAGAAAA GACCACGTAT CACTGAGAAT CGCCAGCACC AGCAGCCATT TCAGGTCTCC 
CCACAGCCTT TTCTTAATAG AGGGGACAGG GTCCAAGTGC GAAAAGTCCC ACCTCTCAAG 
ATCCCTGTCT CCAGAATCTC CCCGATGCTG TTTTCTACAT CACAGGTCTC TCCCAGGGCT 
CGTTTTCCAA TCTCCATCAC TAGTCCTTAC AGAACAGGAG CCAGAACTCT GGCAGACATC 
AAAGCAAAAG CCCAGTTGGT CAAAGCACAA AAGGCAGCAG CCGCAGCAGC TGCTGCAGCT 
GCTGCAGCCG CCTCAGTTGG AGGGACCATT CCGGGACCTG GCCCTGGGGG TGGACAAAGT 
CCAAGAGAGG GTGGTGAAAG GAAAATTGCT GGAGGAGGAA GTGCAGGCTC AGACCCAGTC 
AGTACAAATG GAAAAGGTCC CACACTGGAA CTGGCCGGAA CTGGAAGCAG GGGAGGTACG 
AGAGAACTTT TACCCTGTGG TCCTCAACCT GAGACCAATA TGCCAGGCCA AGCACAGCCT 
CCTGGTATCT CTGGAGCACA ACTACAGCAA ACCTCCTCAG TGCCTACGGG ACTTGCCAGC 
AGTGGAGCAT GCACAAGTGT CCCATTACCA GCCCACATAG AAATATCAAA CAGTGAAAAA 
CCAAACCTCC ACAAGGCAAC AGCCACAGCA GCCTCTCCTT GCCACTTGCA AGACCCTAGG 
AGTTGCAGAC TTGAAAAAGC TCTGTCTCCA ACAGGGCCTC CTCTGATCTC AGGAGCCTCA 
ACTGTTTATT TTGTAGCTGA TGGCACAGTT GAGCCCAAAG CAGGTTCTAA TAAGAATGCA 
CCAAAGCCTT CAGCCTTAGC AAAGACAACT GCTCCTGCTC CACTAGATAT GACTTCCTCT 
CCTGTGACAA CAGCCAGTTT AGAAAAACTC CCTGTACCCC AGATCAGTGG AACTGCAACA 
TCTACTGGAT CAGCTCCATC CTCAAGCACT TTGCCAGCAG CTTCTAGCCT TAAAACCCCA 
GGAACTTCTG CAAATATGAA TGGACCCATT TCAAGAACAA GTTCTAGTAT CCCTGCTAAT 
AATCCTTTAG TTACTCAGCT GCTCCAGGGC AAAGATGTTC CCTTGGAGCA AATTCTGCCT 
AAGCCTCTCA CCAAAATTGA AATGAAAACA GTTCCACTGA CTACAAAAGA GGAAAAAGGC 
ATAGGGATAT TCCCTGGTAT CAGTGTAATG GAAAGTAGCA GCAGAGAGGA AGTTAATGGC 
AGGCAGGCCC ATCTGGCTAT CCCGCAGTTG GGGAAACCCT TGCAAAGTAA ACAGCTTTCC 
CAGGTTCCAA GGCCAGTTTT CACTGCTAAG GACCGGAAGG ACCCCTGCAT TGACACTCAC 
CAGTACCGAG AAGGTCTAAG TAAAACAACG CAAGATCAGC TCTTTCAGAC TCTTATCCAG 
AGGGCTCAGA GACAGAGTGT TCTTTCATTT GTACCGCCCT CTCAGTTCAA CTTTGCTCAC 
TCAGGTTTCC ACTTGGAAGA CATCTCCACA AGTCAGAAGT TCATGCTGGG TTTTGCTGGC 
AGAAGGACAT CCAAGCCTGC AATGGCAGGT CACTATTTAC TTAATATTTC CACCTATGGC 
AGGGGTACAG AGAACATTAA AAGGACCCAT TCTGTAAATC CTGATGATCG ATTTTGTCTA 
AGTAGCCCCA CTGAGGCGTT GAGAATGGGG CATGCAGATT ATAAAAACAC AACTGGAGAA 
ATCAGCAGCA AAGAAGATGA AAGTGATGAA GATCGCGTAG GTGATGAACA GGAGCCTATT 
TCAGTGAAGG AGGAGCCATG GGCTTCCCAG AGTTCTGGCA GGCATCCGCA CCATGGGGAA 
GCTTCATCTA CCAATGATTG TTTAGCTAGC AAGAATGGTA AGACTGAGGC ACCAGTGAGT 
GAGCAAACCA CTTTAGGCCA GGAGAATTAC ATATTCTCTA GAGGCCAAGC ATCTGATGAA 
AAGAGCCTGC CTAGAGATTT TATTCCGGCA GCACATAAAC AGATGACTCA TGCTGTGAGA 
GGTAAGACAG TGTGTAGCAG CCCTGAGCTT TTCAATTCTA CTGCTCTTTC TCTTCCCGCA 
GACAGTCCCA CACATCAGCC TCTACTCCTT CCACCACTGC AAACCCCCAA ATTGTATGGA 
AGCCCCACAC AGATAGGGCC AAGCTATAGA GGCATGATCA ATGTCTCCAC CTCATCAGAC 
ATGGACCATA ACTCTGCTAT ACCAGGCAGC CAGGTATCTA GCAATGTAGG GGACGTCATG 
TCGTTTTCAG TGACTGTCAC TACCATCCCT GCCAGTCAAG CAATGAATCC CAGCAGCCAT 
GGCCAGACAA TTCCTGTTCA GACTTTTCCT GATGACAATA GTATCGAGGA CACACCATCA 
AAATGTTACT GCCGGTTGAA AGCCATGATC ATGTGCAAAG GATGTGGAGC TTTCTGCCAT 
GATGATTGCA TTGGCCCCTC CAAACTCTGT GTCTCCTGCC TTGTTGTTCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515302.1
CDS	23..4276
Translation	MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEISGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR

Target ORF information:

RefSeq Version	XM_006515239.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus additional sex combs like 2 (Drosophila) (Asxl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515239.3

ORF Insert Sequence:

ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
TTAGAAAAAT ACCCTAATAC ACCCATGAGT CATAAAGAAA TTCTTCAGGT TATCCAGAGA 
GAAGGACTGA AAGAAATCAG TGGGACTTCC CCTCTTGCAT GCTTGAATGC AATGCTGCAC 
ACAAACTCCC GAGGAGAAGA AGGCATCTTC TATAAAGTTC CAGGTAGAAT GGGCGTGTAT 
ACTTTGAAGA AAGATGTGCC AGATGGAGTG AAAGAGCTGT CAGAAGGTTC AGAAGAAAGC 
AGTGATGGTC AGTCAGATTC TCAGAGCTCT GAGAACAGCA GCAGCAGTGA TGGTGGCAGC 
AACAAAGAAG GGAGAAAGAG CAGGTGGAAA AGGAAAGTAT CATCTAGACT GTCACATCCA 
CCCTCTCCCC CGTCAGGCTG CCCATCACCC ACCATTCCAG CAAGTAAAGT CATTTCTCCA 
TCACAGAAGC ACAGCAAAAA GGCATTAAAA CAGGCCTTGA AGCAGCAACA ACAGAAGAAG 
AAGCAGCAGC AATGCAGACC AAGCATGTCC ATCTCCAATC AACACCTCTC TCTCAAGACT 
GTCAAAGCAG CCAGTGACTC TGTACCTGCC AAACCTGCCC TATGGGAAGG AAAGCAATCT 
GATGGACAGT CAAGCAGTCC CCAGAACTCA AATTCCAGCT TTTCTTCCTC AGTGAAAGTG 
GAAAGTTCTC TGCTAGGCTT GGGGAAGAAG TCTTTCCAGA GGTCTGACAG ACTCCACACA 
AGACAGATGA AAAGGACTAA ATGTGCTGAC ATTGATGTTG AGACTCCTGA TTCCATTCTG 
GTTAATACTA ATCTTCGAGC ACTGATCAAC AAGCACACCT TTTCAGTTCT TCCTGGAGAC 
TGCCAGCAGC GCCTGCTTTT GTTGCTTCCA GAGGTGGATC GACAGGTTGG TCCAGATGGC 
CTGATGAAGT TGAATGGCTC AGCCCTTAAC AATGAGTTCT TCACTTCTGC AGCCCAGGGC 
TGGAAGGAAA GGCTCTCAGA AGGTGAATTT ACTCCTGAAA TGCAGGTGAG AATTCGACAA 
GAGATTGAGA AGGAGAAGAA AGTAGAGCTG TGGAAGGAAC AATTCTTTGA AAATTACTAT 
GGTCAGAGTT CTGGCCTAAG CCTTGAAGAT TCACAGAAAC TGACAGCTTC CTCAAGTGAT 
CCCAAAGCAA AGAAAACCCC AGCTGAGCAA CCAAAATCCA TACTTCCTTC AGAGGCCTCT 
CCTGTCAGAA TTGTCCCAGT AGTTCCTCAG TCCGAGTGTA AAGAAGAAGC AGTGCAGATA 
CCATCACCAT CCCAAAAGGA AGAAAATCAA GACGAGGCGA GGCCAGACTC CAAATCCCCA 
GAACCTGTTC TTGCATCAGC TAGCAATACA AATGAGCTTA TCACCATGAA ACCCATCAAG 
AGCCCTAAGG ATGAGGGTCT CTTAGAGCAG AAGCCTGTTG CCTGTGCTGA ACAAGAATCT 
GAGAAAGAAA ATCATGTAAC CACAACTTCT CGTAATAACA AAAGTGAGAA CCAAGAAGCG 
TTAGCTATAT CCCCAAGCAA ATCTAAAAAT GCTGGGTTAC AAAAACCCAT AATAAAGCCT 
GTTGCAGAAG CCAGTCCACT GAACCCTGAT ATGAAGATGC CACCAGCAAC TGTTACTGAT 
CAGATCCAAG AAAGTCTCAA GAGGAAATCT TCTCTTACTG ATGAAGAGGC CACCAGTAGC 
TGGGAGAAAA GACCACGTAT CACTGAGAAT CGCCAGCACC AGCAGCCATT TCAGGTCTCC 
CCACAGCCTT TTCTTAATAG AGGGGACAGG GTCCAAGTGC GAAAAGTCCC ACCTCTCAAG 
ATCCCTGTCT CCAGAATCTC CCCGATGCTG TTTTCTACAT CACAGGTCTC TCCCAGGGCT 
CGTTTTCCAA TCTCCATCAC TAGTCCTTAC AGAACAGGAG CCAGAACTCT GGCAGACATC 
AAAGCAAAAG CCCAGTTGGT CAAAGCACAA AAGGCAGCAG CCGCAGCAGC TGCTGCAGCT 
GCTGCAGCCG CCTCAGTTGG AGGGACCATT CCGGGACCTG GCCCTGGGGG TGGACAAAGT 
CCAAGAGAGG GTGGTGAAAG GAAAATTGCT GGAGGAGGAA GTGCAGGCTC AGACCCAGTC 
AGTACAAATG GAAAAGGTCC CACACTGGAA CTGGCCGGAA CTGGAAGCAG GGGAGGTACG 
AGAGAACTTT TACCCTGTGG TCCTCAACCT GAGACCAATA TGCCAGGCCA AGCACAGCCT 
CCTGGTATCT CTGGAGCACA ACTACAGCAA ACCTCCTCAG TGCCTACGGG ACTTGCCAGC 
AGTGGAGCAT GCACAAGTGT CCCATTACCA GCCCACATAG AAATATCAAA CAGTGAAAAA 
CCAAACCTCC ACAAGGCAAC AGCCACAGCA GCCTCTCCTT GCCACTTGCA AGACCCTAGG 
AGTTGCAGAC TTGAAAAAGC TCTGTCTCCA ACAGGGCCTC CTCTGATCTC AGGAGCCTCA 
ACTGTTTATT TTGTAGCTGA TGGCACAGTT GAGCCCAAAG CAGGTTCTAA TAAGAATGCA 
CCAAAGCCTT CAGCCTTAGC AAAGACAACT GCTCCTGCTC CACTAGATAT GACTTCCTCT 
CCTGTGACAA CAGCCAGTTT AGAAAAACTC CCTGTACCCC AGATCAGTGG AACTGCAACA 
TCTACTGGAT CAGCTCCATC CTCAAGCACT TTGCCAGCAG CTTCTAGCCT TAAAACCCCA 
GGAACTTCTG CAAATATGAA TGGACCCATT TCAAGAACAA GTTCTAGTAT CCCTGCTAAT 
AATCCTTTAG TTACTCAGCT GCTCCAGGGC AAAGATGTTC CCTTGGAGCA AATTCTGCCT 
AAGCCTCTCA CCAAAATTGA AATGAAAACA GTTCCACTGA CTACAAAAGA GGAAAAAGGC 
ATAGGGATAT TCCCTGGTAT CAGTGTAATG GAAAGTAGCA GCAGAGAGGA AGTTAATGGC 
AGGCAGGCCC ATCTGGCTAT CCCGCAGTTG GGGAAACCCT TGCAAAGTAA ACAGCTTTCC 
CAGGTTCCAA GGCCAGTTTT CACTGCTAAG GACCGGAAGG ACCCCTGCAT TGACACTCAC 
CAGTACCGAG AAGGTCTAAG TAAAACAACG CAAGATCAGC TCTTTCAGAC TCTTATCCAG 
AGGGCTCAGA GACAGAGTGT TCTTTCATTT GTACCGCCCT CTCAGTTCAA CTTTGCTCAC 
TCAGGTTTCC ACTTGGAAGA CATCTCCACA AGTCAGAAGT TCATGCTGGG TTTTGCTGGC 
AGAAGGACAT CCAAGCCTGC AATGGCAGGT CACTATTTAC TTAATATTTC CACCTATGGC 
AGGGGTACAG AGAACATTAA AAGGACCCAT TCTGTAAATC CTGATGATCG ATTTTGTCTA 
AGTAGCCCCA CTGAGGCGTT GAGAATGGGG CATGCAGATT ATAAAAACAC AACTGGAGAA 
ATCAGCAGCA AAGAAGATGA AAGTGATGAA GATCGCGTAG GTGATGAACA GGAGCCTATT 
TCAGTGAAGG AGGAGCCATG GGCTTCCCAG AGTTCTGGCA GGCATCCGCA CCATGGGGAA 
GCTTCATCTA CCAATGATTG TTTAGCTAGC AAGAATGGTA AGACTGAGGC ACCAGTGAGT 
GAGCAAACCA CTTTAGGCCA GGAGAATTAC ATATTCTCTA GAGGCCAAGC ATCTGATGAA 
AAGAGCCTGC CTAGAGATTT TATTCCGGCA GCACATAAAC AGATGACTCA TGCTGTGAGA 
GGTAAGACAG TGTGTAGCAG CCCTGAGCTT TTCAATTCTA CTGCTCTTTC TCTTCCCGCA 
GACAGTCCCA CACATCAGCC TCTACTCCTT CCACCACTGC AAACCCCCAA ATTGTATGGA 
AGCCCCACAC AGATAGGGCC AAGCTATAGA GGCATGATCA ATGTCTCCAC CTCATCAGAC 
ATGGACCATA ACTCTGCTAT ACCAGGCAGC CAGGTATCTA GCAATGTAGG GGACGTCATG 
TCGTTTTCAG TGACTGTCAC TACCATCCCT GCCAGTCAAG CAATGAATCC CAGCAGCCAT 
GGCCAGACAA TTCCTGTTCA GACTTTTCCT GATGACAATA GTATCGAGGA CACACCATCA 
AAATGTTACT GCCGGTTGAA AGCCATGATC ATGTGCAAAG GATGTGGAGC TTTCTGCCAT 
GATGATTGCA TTGGCCCCTC CAAACTCTGT GTCTCCTGCC TTGTTGTTCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Asxl2
Entrez Gene ID	75302
Full Name	additional sex combs like 2, transcriptional regulator
Synonyms	4930556B16Rik,mKIAA1685
General protein information	Preferred Names additional sex combs like 2, transcriptional regulator Names putative Polycomb group protein ASXL2 additional sex combs like 2 additional sex combs-like protein 2 polycomb group protein ASXH2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A1.1
Summary	This gene encodes a homolog of the Drosophila Asx gene, which interacts with genes involved in axial patterning. Mice with mutations in this gene display abnormal patterning of the axial skeleton, suggesting a similar function in mice as in Drosophila. This gene may also be involved in bone mineral density, specifically osteoclastogenesis. [provided by RefSeq, Aug 2012].

Canis lupus familiaris (dog)	ASXL2	XP_003432208.1
Mus musculus (house mouse)	Asxl2	NP_001257917.1
Gallus gallus (chicken)	ASXL2	NP_001026267.1
Danio rerio (zebrafish)	LOC100150267	XP_005158913.1
Homo sapiens (human)	ASXL2	NP_060733.4
	ASXL2	XP_001084149.1
Bos taurus (cattle)	ASXL2	XP_005213137.1
Pan troglodytes (chimpanzee)	ASXL2	XP_515337.3
Rattus norvegicus (Norway rat)	Asxl2	XP_003750168.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Asxl2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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