Usp50 cDNA ORF clone, Mus musculus(house mouse)

The following Usp50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu06598 NM_029163.3
Latest version!
Mus musculus ubiquitin specific peptidase 50 (Usp50), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu06598
    Clone ID Related Accession (Same CDS sequence) NM_029163.3
    Accession Version NM_029163.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Mus musculus(house mouse)
    Product putative ubiquitin carboxyl-terminal hydrolase 50
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY707071.1, AK015766.1 and AW539155.2. On Oct 1, 2009 this sequence version replaced NM_029163.2. ##Evidence-Data-START## Transcript exon combination :: AK015766.1, BC061020.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTGCTTCA TTGACATGGC GTCTCATCGG CCGGTCCCTG CAGACGACTT CGGTGCCTAC 
    TACAACCTTG CGGAGTGTGC AGACTATGAC TCCCTGCCGG AGTCTAAGAC CCAGCCACAC
    TTCCAGGGCG TGACGGGACT GCGGAACCTG GGCAACACCT GCTACATGAA CGCCATCTTG
    CAATGCCTCT GCAGCGTATC TCCACTGGTG GAGTACTTCC TGTCCGGAAA GTACATCACA
    GCTCTTAAGA AGGACTGCAG TGAGGTTACC ACCGCCTTTG CCTACCTGAT GACAGATATG
    TGGCTTGGGG ACTCAGACTG TGTCTCACCA GAGATATTTC TGTCGGCTGT TGGCAGTCTT
    TACCCAGCTT TTCTGAAAAA GACACAGCAA GATGCGCAGG AGTTCCTGAT TTACGTCCTC
    AACGAGCTTC ACGAAGCTTT GAAAAAGCAC TGCCGAAGAA GAGTGAATGA GAAAAGAACA
    GGCCAAAGCT GCTGCAGGAA GGTGCCGGCC CAGGAGACCT CCATCATCAC CCGGCTGTTT
    GAAGGACAGC TCAGTTACAG CATCACATGT TTAAAGTGTG AGAGCTGCAC GCACAAGAAC
    GAAGTTTTCA CCATCCTCTC CCTCCCCATC CCATCTGACT ATGAATGTTC CCTCCAGGAC
    TGTCTCCAGT GTTTTTTCCA ACAGGACACA CTGACCTGGA GCAACCAAAT CTATTGTTCC
    TTTTGTGAAA TCAAGCAAGA GGCAGCTGTG AGGACCACGA TTTCCAAGGT GCCGAAAATA
    ATTGTTTTTC ACTTTAAAAG GTTTGACATC CAGGGCACAG TAAAGAGGAA GCTGCGGACA
    GACATCCATT ACCCCCTGAC CAACCTGGAC CTCACTCCTT ATATCTGTCC AGTTTTCCGG
    AAACATCCCA TGTACAACCT GTGCGCAGTG GTGAACCACT TTGGAGATCT GGATGGTGGT
    CACTACACTG CTTTCTGCAA GAACTCGGTA ACTCAGGCCT GGTACAGCTT TGATGACACC
    CGAGTCAGTG AGATCCCAGA TACTTCTGTT CAAACAGCTA CAGCCTATCT TCTGTTCTAT
    AGCTGCCAGC CTTTTTCTAT ACCAGCACAG AAACGCAAGA GCCAGGACAG TACTCCTGAC
    CACTGCAAGC AAGCTATCCG AAAGTGGCCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_083439.2
    CDS174..1346
    Translation

    Target ORF information:

    RefSeq Version NM_029163.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus ubiquitin specific peptidase 50 (Usp50), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_029163.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTGCTTCA TTGACATGGC GTCTCATCGG CCGGTCCCTG CAGACGACTT CGGTGCCTAC 
    TACAACCTTG CGGAGTGTGC AGACTATGAC TCCCTGCCGG AGTCTAAGAC CCAGCCACAC
    TTCCAGGGCG TGACGGGACT GCGGAACCTG GGCAACACCT GCTACATGAA CGCCATCTTG
    CAATGCCTCT GCAGCGTATC TCCACTGGTG GAGTACTTCC TGTCCGGAAA GTACATCACA
    GCTCTTAAGA AGGACTGCAG TGAGGTTACC ACCGCCTTTG CCTACCTGAT GACAGATATG
    TGGCTTGGGG ACTCAGACTG TGTCTCACCA GAGATATTTC TGTCGGCTGT TGGCAGTCTT
    TACCCAGCTT TTCTGAAAAA GACACAGCAA GATGCGCAGG AGTTCCTGAT TTACGTCCTC
    AACGAGCTTC ACGAAGCTTT GAAAAAGCAC TGCCGAAGAA GAGTGAATGA GAAAAGAACA
    GGCCAAAGCT GCTGCAGGAA GGTGCCGGCC CAGGAGACCT CCATCATCAC CCGGCTGTTT
    GAAGGACAGC TCAGTTACAG CATCACATGT TTAAAGTGTG AGAGCTGCAC GCACAAGAAC
    GAAGTTTTCA CCATCCTCTC CCTCCCCATC CCATCTGACT ATGAATGTTC CCTCCAGGAC
    TGTCTCCAGT GTTTTTTCCA ACAGGACACA CTGACCTGGA GCAACCAAAT CTATTGTTCC
    TTTTGTGAAA TCAAGCAAGA GGCAGCTGTG AGGACCACGA TTTCCAAGGT GCCGAAAATA
    ATTGTTTTTC ACTTTAAAAG GTTTGACATC CAGGGCACAG TAAAGAGGAA GCTGCGGACA
    GACATCCATT ACCCCCTGAC CAACCTGGAC CTCACTCCTT ATATCTGTCC AGTTTTCCGG
    AAACATCCCA TGTACAACCT GTGCGCAGTG GTGAACCACT TTGGAGATCT GGATGGTGGT
    CACTACACTG CTTTCTGCAA GAACTCGGTA ACTCAGGCCT GGTACAGCTT TGATGACACC
    CGAGTCAGTG AGATCCCAGA TACTTCTGTT CAAACAGCTA CAGCCTATCT TCTGTTCTAT
    AGCTGCCAGC CTTTTTCTAT ACCAGCACAG AAACGCAAGA GCCAGGACAG TACTCCTGAC
    CACTGCAAGC AAGCTATCCG AAAGTGGCCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The deubiquitinating enzyme, ubiquitin-specific peptidase 50, regulates inflammasome activation by targeting the ASC adaptor protein.
    FEBS letters591(3)479-490(2017 02)
    Lee JY,Seo D,You J,Chung S,Park JS,Lee JH,Jung SM,Lee YS,Park SH


    High-throughput discovery of novel developmental phenotypes.
    Nature537(7621)508-514(2016 09)
    Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA