Selenon cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Selenon
Entrez Gene ID	74777
Full Name	selenoprotein N
Synonyms	1110019I12Rik,AI414492,SelN,Sepn1
General protein information	Preferred Names selenoprotein N Names selenoprotein N selenoprotein N, 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D3
Summary	This gene encodes a glycoprotein that is localized in the endoplasmic reticulum. It plays an important role in cell protection against oxidative stress, and in the regulation of redox-related calcium homeostasis. Mutations in the orthologous gene in human are associated with early onset muscle disorders, referred to as SEPN1-related myopathy. Knockout mice deleted for this gene exhibit abnormal lung development. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A second stop-codon redefinition element (SRE) adjacent to the UGA codon has been identified in this gene (PMID:15791204). SRE is a phylogenetically conserved stem-loop structure that stimulates readthrough at the UGA codon, and augments the Sec insertion efficiency by SECIS. [provided by RefSeq, Dec 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_029100.2	NP_083376.2	selenoprotein N precursor

Pathways from BioSystems

Homologies

	SEPN1	XP_002802303.1
Danio rerio (zebrafish)	sepn1	NP_001004294.4
Xenopus tropicalis (tropical clawed frog)	sepn1	NP_001070901.1
Rattus norvegicus (Norway rat)	Sepn1	XP_006239194.1
Gallus gallus (chicken)	SEPN1	NP_001108444.1
Bos taurus (cattle)	SEPN1	NP_001108448.1
Mus musculus (house mouse)	Sepn1	NP_083376.2
Homo sapiens (human)	SEPN1	NP_996809.1
Pan troglodytes (chimpanzee)	SEPN1	XP_003307954.2
Canis lupus familiaris (dog)	SEPN1	NP_001108447.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein N Was Required for the Regulation of Selenium on the Uterine Smooth Muscle Contraction in Mice.
Zhou J, Li C, Gu G, Wang Q, Guo M
Biological trace element research(2017 Aug)

Endoplasmic Reticulum Oxidative Stress Triggers Tgf-Beta-Dependent Muscle Dysfunction by Accelerating Ascorbic Acid Turnover.
Pozzer D, Favellato M, Bolis M, Invernizzi RW, Solagna F, Blaauw B, Zito E
Scientific reports740993(2017 Jan)

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

SEPN1, an endoplasmic reticulum-localized selenoprotein linked to skeletal muscle pathology, counteracts hyperoxidation by means of redox-regulating SERCA2 pump activity.
Marino M, Stoilova T, Giorgi C, Bachi A, Cattaneo A, Auricchio A, Pinton P, Zito E
Human molecular genetics24(7)1843-55(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SEPN1 enhances SERCA2 activity by reducing luminal cysteines that are hyperoxidized by ERO1-generated peroxides.
Title: SEPN1, an endoplasmic reticulum-localized selenoprotein linked to skeletal muscle pathology, counteracts hyperoxidation by means of redox-regulating SERCA2 pump activity.

SepN deficiency leads to abnormal lung development characterized by enlarged alveoli, which is associated with decreased tissue elastance and increased quasi-static compliance of Sepn1(-/-) lungs.
Title: Selenoprotein N deficiency in mice is associated with abnormal lung development.

we describe for the first time a major physiological function of SelN in skeletal muscles, as a key regulator of SC function, which likely plays a central role in the pathophysiological mechanism leading to SEPN1-RM.
Title: Satellite cell loss and impaired muscle regeneration in selenoprotein N deficiency.

Data show that Sepn1(-/-) mice developed an obvious phenotype, characterized by limited motility and body rigidity during the swimming session.
Title: Increased muscle stress-sensitivity induced by selenoprotein N inactivation in mouse: a mammalian model for SEPN1-related myopathy.

In whole embryos, Sepn1 transcripts were detected as early as E5.5, with expression levels peaking at E12.5, and then strongly decreasing until birth.
Title: Selenoprotein N is dynamically expressed during mouse development and detected early in muscle precursors.

The following Selenon gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Selenon cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06530	NM_029100.2 Latest version!	Mus musculus selenoprotein N (Selenon), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Selenon ( NM_029100.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083376.2 Mus musculus selenoprotein N (Selenon), mRNA.

Price & Availability↑

CloneID	OMu06530
Clone ID Related Accession (Same CDS sequence)	NM_029100.2
Accession Version	NM_029100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	selenoprotein N precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY742289.1, CX562316.1, CF737950.1, AK003819.1, BU057838.1 and BU756654.1. On Aug 2, 2006 this sequence version replaced NM_029100.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCCAGG CCAGGCCCGC AGCGCGCCGC CCGCACAGCC CGGACCCCGG CGCTCAGCCC 
GCGCCTCCAC GCCGCCGCGC CCGCGCCCTG GCGCTGCTCG GAGCCCTGCT GGCGGCCGCC 
GCCGCTGTCG CCGCTGCCCG GGCCTGCGCG CTCCTCGCCG ACGCCCAGGC GGCCGCGCGG 
CAGGAATCGG CATTAAAGGT CCTGGGGACA GATGGCCTCT TTCTCTTTTC CTCGCTGGAC 
ACTGACCAGG ATATGTACAT CAGTCCGGAG GAATTCAAAC CCATTGCGGA GAAACTGACA 
GGGTCAGTTC CTGTGGCCAA CTACGAGGAG GAGGAACTGC CCCATGACCC CAGCGAGGAG 
ACTCTTACCA TAGAAGCCCG ATTCCAGCCT CTGCTCATGG AGACCATGAC CAAAAGCAAA 
GATGGCTTCC TAGGGGTCTC CCGCCTCGCT CTGTCCGGCC TCCGCAACTG GACCACAGCA 
GCCTCGCCGA GTGCAGCGTT TGCTGCCCGC CACTTCCGGC CCTTCCTGCC CCCTCCAGGT 
CAGGAGCTGG GCCAGCCCTG GTGGATTATC CCCGGGGAGC TGAGCGTCTT CACGGGCTAT 
TTGTCCAACA ACCGCTTCTA CCCACCACCG CCCAAGGGCA AGGAGGTCAT CATCCATCGC 
CTGTTAAGCA TGTTCCATCC ACGCCCCTTC GTGAAGACCC GCTTTGCCCC TCAGGGCACC 
GTGGCCTGTC TGACTGCCAT CAGCGATTCC TACTACACCG TGATGTTCCG GATCCACGCG 
GAGTTTCAGC TCAGCGAGCC TCCTGACTTC CCCTTCTGGT TCTCGCCCGG CCAGTTCACC 
GGCCATATCA TCTTATCCAA AGATGCTACA CACATCCGTG ACTTCAGGCT GTTTGTGCCC 
AATCACAGGT CCCTGAATGT GGACATGGAG TGGCTGTATG GGGCCAGTGA GACCAGCAAC 
ATGGAGGTGG ACATTGGCTA CGTCCCCCAG ATGGAGCTGG AGGCTGTGGG CCCCTCGGTG 
CCCTCTGTGA TCCTAGATGA GGACGGCAAC ATGATTGACA GCCGCCTGCC CTCAGGAGAA 
CCCCTCCAGT TTGTGTTTGA GGAGATCAAG TGGCATCAGG AGCTGAGCTG GGAAGAGGCT 
GCCCGGCGCC TGGAGGTGGC CATGTATCCC TTCAAGAAGG TCAACTACCT GCCGTTCACG 
GAGGCCTTTG ACAGAGCCAG AGCTGAGAAG AAACTTGTTC ATTCCATCTT GCTGTGGGGG 
GCCCTGGACG ACCAGTCCTG CTGAGGTTCC GGGCGGACTC TCCGGGAGAC TGTCCTGGAA 
AGCCCGCCCA TCCTCACTCT CCTCAATGAG AGCTTCATCA GTACCTGGTC CCTGGTAAAG 
GAGCTAGAAG ACCTGCAGAC CCAGCAGGAG AACCCACTCC ACAGGCAGCT GGCAGGCCTG 
CACTTGGAGA AGTACAGCTT TCCTGTAGAG ATGATGATCT GTCTGCCCAA CGGCACTGTG 
GTCCACCACA TCAATGCCAA CTACTTCCTT GACATCACCT CCATGAAGCC TGAAGACATG 
GAGAATAATA ACGTCTTCAG CTTCTCATCC AGCTTTGAGG ACCCGTCCAC AGCTACCTAC 
ATGCAGTTCC TGAGGGAAGG ACTCCGGCGG GGCCTGCCCC TCCTCCAGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083376.2
CDS	48..1721
Translation	MGQARPAARRPHSPDPGAQPAPPRRRARALALLGALLAAAAAVAAARACALLADAQAAARQESALKVLGTDGLFLFSSLDTDQDMYISPEEFKPIAEKLTGSVPVANYEEEELPHDPSEETLTIEARFQPLLMETMTKSKDGFLGVSRLALSGLRNWTTAASPSAAFAARHFRPFLPPPGQELGQPWWIIPGELSVFTGYLSNNRFYPPPPKGKEVIIHRLLSMFHPRPFVKTRFAPQGTVACLTAISDSYYTVMFRIHAEFQLSEPPDFPFWFSPGQFTGHIILSKDATHIRDFRLFVPNHRSLNVDMEWLYGASETSNMEVDIGYVPQMELEAVGPSVPSVILDEDGNMIDSRLPSGEPLQFVFEEIKWHQELSWEEAARRLEVAMYPFKKVNYLPFTEAFDRARAEKKLVHSILLWGALDDQSC*GSGRTLRETVLESPPILTLLNESFISTWSLVKELEDLQTQQENPLHRQLAGLHLEKYSFPVEMMICLPNGTVVHHINANYFLDITSMKPEDMENNNVFSFSSSFEDPSTATYMQFLREGLRRGLPLLQP

Target ORF information:

RefSeq Version	NM_029100.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus selenoprotein N (Selenon), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029100.2

ORF Insert Sequence:

ATGGGCCAGG CCAGGCCCGC AGCGCGCCGC CCGCACAGCC CGGACCCCGG CGCTCAGCCC 
GCGCCTCCAC GCCGCCGCGC CCGCGCCCTG GCGCTGCTCG GAGCCCTGCT GGCGGCCGCC 
GCCGCTGTCG CCGCTGCCCG GGCCTGCGCG CTCCTCGCCG ACGCCCAGGC GGCCGCGCGG 
CAGGAATCGG CATTAAAGGT CCTGGGGACA GATGGCCTCT TTCTCTTTTC CTCGCTGGAC 
ACTGACCAGG ATATGTACAT CAGTCCGGAG GAATTCAAAC CCATTGCGGA GAAACTGACA 
GGGTCAGTTC CTGTGGCCAA CTACGAGGAG GAGGAACTGC CCCATGACCC CAGCGAGGAG 
ACTCTTACCA TAGAAGCCCG ATTCCAGCCT CTGCTCATGG AGACCATGAC CAAAAGCAAA 
GATGGCTTCC TAGGGGTCTC CCGCCTCGCT CTGTCCGGCC TCCGCAACTG GACCACAGCA 
GCCTCGCCGA GTGCAGCGTT TGCTGCCCGC CACTTCCGGC CCTTCCTGCC CCCTCCAGGT 
CAGGAGCTGG GCCAGCCCTG GTGGATTATC CCCGGGGAGC TGAGCGTCTT CACGGGCTAT 
TTGTCCAACA ACCGCTTCTA CCCACCACCG CCCAAGGGCA AGGAGGTCAT CATCCATCGC 
CTGTTAAGCA TGTTCCATCC ACGCCCCTTC GTGAAGACCC GCTTTGCCCC TCAGGGCACC 
GTGGCCTGTC TGACTGCCAT CAGCGATTCC TACTACACCG TGATGTTCCG GATCCACGCG 
GAGTTTCAGC TCAGCGAGCC TCCTGACTTC CCCTTCTGGT TCTCGCCCGG CCAGTTCACC 
GGCCATATCA TCTTATCCAA AGATGCTACA CACATCCGTG ACTTCAGGCT GTTTGTGCCC 
AATCACAGGT CCCTGAATGT GGACATGGAG TGGCTGTATG GGGCCAGTGA GACCAGCAAC 
ATGGAGGTGG ACATTGGCTA CGTCCCCCAG ATGGAGCTGG AGGCTGTGGG CCCCTCGGTG 
CCCTCTGTGA TCCTAGATGA GGACGGCAAC ATGATTGACA GCCGCCTGCC CTCAGGAGAA 
CCCCTCCAGT TTGTGTTTGA GGAGATCAAG TGGCATCAGG AGCTGAGCTG GGAAGAGGCT 
GCCCGGCGCC TGGAGGTGGC CATGTATCCC TTCAAGAAGG TCAACTACCT GCCGTTCACG 
GAGGCCTTTG ACAGAGCCAG AGCTGAGAAG AAACTTGTTC ATTCCATCTT GCTGTGGGGG 
GCCCTGGACG ACCAGTCCTG CTGAGGTTCC GGGCGGACTC TCCGGGAGAC TGTCCTGGAA 
AGCCCGCCCA TCCTCACTCT CCTCAATGAG AGCTTCATCA GTACCTGGTC CCTGGTAAAG 
GAGCTAGAAG ACCTGCAGAC CCAGCAGGAG AACCCACTCC ACAGGCAGCT GGCAGGCCTG 
CACTTGGAGA AGTACAGCTT TCCTGTAGAG ATGATGATCT GTCTGCCCAA CGGCACTGTG 
GTCCACCACA TCAATGCCAA CTACTTCCTT GACATCACCT CCATGAAGCC TGAAGACATG 
GAGAATAATA ACGTCTTCAG CTTCTCATCC AGCTTTGAGG ACCCGTCCAC AGCTACCTAC 
ATGCAGTTCC TGAGGGAAGG ACTCCGGCGG GGCCTGCCCC TCCTCCAGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein N Was Required for the Regulation of Selenium on the Uterine Smooth Muscle Contraction in Mice.
Biological trace element research(2017 Aug)
Zhou J,Li C,Gu G,Wang Q,Guo M

Endoplasmic Reticulum Oxidative Stress Triggers Tgf-Beta-Dependent Muscle Dysfunction by Accelerating Ascorbic Acid Turnover.
Scientific reports740993(2017 Jan)
Pozzer D,Favellato M,Bolis M,Invernizzi RW,Solagna F,Blaauw B,Zito E

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

SEPN1, an endoplasmic reticulum-localized selenoprotein linked to skeletal muscle pathology, counteracts hyperoxidation by means of redox-regulating SERCA2 pump activity.
Human molecular genetics24(7)1843-55(2015 Apr)
Marino M,Stoilova T,Giorgi C,Bachi A,Cattaneo A,Auricchio A,Pinton P,Zito E

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Selenon cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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