Wdr35 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wdr35
Entrez Gene ID	74682
Full Name	WD repeat domain 35
Synonyms	4930459M12Rik,4931430C06,mKIAA1336
General protein information	Preferred Names WD repeat domain 35 Names WD repeat-containing protein 35
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159527.1	NP_001152999.1	WD repeat-containing protein 35 isoform 2
XM_006515232.2	XP_006515295.1	WD repeat-containing protein 35 isoform X1
NM_172470.3	NP_766058.3	WD repeat-containing protein 35 isoform 1
XM_006515232.3	XP_006515295.1	WD repeat-containing protein 35 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WDR35	XP_515315.3
Bos taurus (cattle)	WDR35	XP_002691565.2
Xenopus tropicalis (tropical clawed frog)	wdr35	XP_002934527.2
Mus musculus (house mouse)	Wdr35	NP_766058.3
Danio rerio (zebrafish)	wdr35	NP_001139061.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011562	XP_309945.3
Homo sapiens (human)	WDR35	NP_001006658.1
Canis lupus familiaris (dog)	WDR35	XP_005630221.1
Gallus gallus (chicken)	WDR35	XP_419970.3
Caenorhabditis elegans (roundworm)	ifta-1	NP_508940.3
	WDR35	XP_001107482.2
Rattus norvegicus (Norway rat)	Wdr35l	NP_001092810.1
Drosophila melanogaster (fruit fly)	Oseg4	NP_001261278.1

Gene Ontology
Bibliography

Related articles in PubMed

Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium.
Caparr?s-Mart?n JA, De Luca A, Cartault F, Aglan M, Temtamy S, Otaify GA, Mehrez M, Valencia M, V?zquez L, Alessandri JL, Nevado J, Rueda-Arenas I, Heath KE, Digilio MC, Dallapiccola B, Goodship JA, Mill P, Lapunzina P, Ruiz-Perez VL
Human molecular genetics24(14)4126-37(2015 Jul)

Enhanced expression of WD repeat-containing protein 35 via nuclear factor-kappa B activation in bupivacaine-treated Neuro2a cells.
Huang L, Kondo F, Harato M, Feng GG, Ishikawa N, Fujiwara Y, Okada S
PloS one9(1)e86336(2014)

Enhanced expression of WD repeat-containing protein 35 via CaMKK/AMPK activation in bupivacaine-treated Neuro2a cells.
Huang L, Kondo F, Gosho M, Feng GG, Harato M, Xia ZY, Ishikawa N, Fujiwara Y, Okada S
PloS one9(5)e98185(2014)

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
Nakano Y, Jahan I, Bonde G, Sun X, Hildebrand MS, Engelhardt JF, Smith RJ, Cornell RA, Fritzsch B, B?nfi B
PLoS genetics8(10)e1002966(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C-Jun siRNA did not inhibit the bupivacaine-induced increase in WDR35 mRNA expression.
Title: Enhanced expression of WD repeat-containing protein 35 via nuclear factor-kappa B activation in bupivacaine-treated Neuro2a cells.

These results suggest that bupivacaine activates AMPK and p38 MAPK via CaMKK in Neuro2a cells, and that the CaMKK/AMPK/p38 MAPK pathway is involved in regulating WDR35 expression.
Title: Enhanced expression of WD repeat-containing protein 35 via CaMKK/AMPK activation in bupivacaine-treated Neuro2a cells.

In a mutation screen for developmental phenotypes, we identified a mutation in Wdr35 as the cause of midgestation lethality, with abnormalities characteristic of defects in the Hedgehog signaling pathway.
Title: Human and mouse mutations in WDR35 cause short-rib polydactyly syndromes due to abnormal ciliogenesis.

The following Wdr35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wdr35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu47424	XM_006515232.3 Latest version!	Mus musculus WD repeat domain 35 (Wdr35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu22548	NM_172470.3 Latest version!	Mus musculus WD repeat domain 35 (Wdr35), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu22587	NM_001159527.1 Latest version!	Mus musculus WD repeat domain 35 (Wdr35), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$692.30 $989.00
View Old Accession Versions >>
OMu47424	XM_006515232.2	Mus musculus WD repeat domain 35 (Wdr35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wdr35 ( XM_006515232.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515295.1 Mus musculus WD repeat domain 35 (Wdr35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47424
Clone ID Related Accession (Same CDS sequence)	XM_006515232.2 , XM_006515232.3
Accession Version	XM_006515232.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	WD repeat-containing protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006515232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGA AGCTGAATTG CTTGGTGAAC GTCACTGGAG CGATCAGTAT CGCTGGGATT 
CACTGGTACC ATGGCACCGA AGGCTACGTG GAGCCCGATT GCCCTTGCCT GGCAATTTGC 
TTTGACAATG GGAGATGCCA AATAATGCGG CATGAGAATG ACCAAAACCC GGTTTTGATC 
GACACGGGCA TGTATGTGGT AGGCATCCAG TGGAACCACA TCGGCAGTGT GTTAGCTGTG 
GCCGGCTCCC AGAAGGTAGT CACTCAGGAC AAGGACATCA ACATCGTGCA GTTCTACACA 
CCGTTCGGTG AGCATCTGGG TACTTTGAAA GTTCCAGGAA AGCAGATGTG TTCGCTGTCT 
TGGGAAGGAG GCGGACTGAA GATTGCTCTA GCTGTTGACT CCTTTATATA TTTTGCAAAT 
ATTCGACCTG ATTATAAGTG GGGCTACTGT TCCAACACAG TGGTTTATGC GTACACCAGA 
CCCGATCGTC CCGAATACTG TGTTGTGTTC TGGGACACAA AAAACAGTGA GAAATACGTT 
AAATATGTGA AGAGCCTCAT TTCTATCACC ACGTGTGGAG ATTTCTGCAT TTTGGCTACA 
AAAGCTGATG AGAATCACCC TCAGGAGGAG AATGAAATGG AGACATTTGG TGCAACGTTT 
GTGCTTGTCC TTTGCAATTC TATTGGCACA CCCTTGGACC CTAAATACAT TGATCTTGTA 
CCATTATTTG TTGCAATGAC CAAAACCCAT GTGATAGCAG CTTCAAAAGA AGCATTTTAT 
ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC ACAGCACTGG AAATTAATCA GATCACACGG 
TCTCGAAAAG AAGGAAGAGA AAGAATTTAT CACGTTGATG ATGTCCCTTC CGGATCTGTG 
GATGGGGTGT TTGATTACAG TAAAGCCATT CAAGGTACAA GGGATCCAAT TTGTGCCATT 
ACTGCATCTG ATAAGACATT GATTGTGGGG CGTGAATCTG GCGTCATTCA GAGGTACAGC 
TTTCCTAATG TTGCTCTGAT TCAAAAGTAT TCCCTGGATT GCCGTGCCTG CCAGTTATCT 
CTGAACTGCA ACTCCAGTCG TCTTGCTATC ATCGACATCG CTGGAGTCTT GACTTTCTTT 
GACTTGGACA CTCGGGTGAC CGACAGTACA GGGCAGCAGG TCGTTGGCGA GTTGCTAAAA 
CTGGAGCGCA AAGACGTCTG GGATATGAAG TGGGCCAAGG ACAATCCTGA TTTGTTTGCA 
ATGATGGAGA AGACGAGAAT GTATGTTTTC AGAAACTTGG ATCCTGAGGA ACCCATTCAG 
ACCTCTGGAT ATATTTGCAA CTTTGAGGAT TTGGAAATTA AGTCTGTTCT CTTGGATGAG 
ATACTGAAGG ACCCAGAGCA CCCTAGCAAG GATTACATAA TGAACTTTGA GATCCGGTCC 
CTGCGAGACA GCCGAGCATT GATTGAGAAA GTTGGAATTG AAGATGCGTC TCAGTTCATA 
GAGGACAACC CACACCCCCG ACTTTGGCGC CTGCTGGCTG AAGCTGCTCT CCAGAAGCTG 
GACCTGTACA CCGCACAGCA AGCGTTTGTG CGCTGCAAGG ATTACCAAGG CATCAAGTTT 
GTGAAGCTCC TGGGCAATCT GCAGAGCGAG TCAATGAAGC AGGCTGAAGT TATTGCCTAC 
TTTGGCAGGT TCGAAGATGC CGAAAGGATG TATCAAGATA TGGACAGACG GGACCTCGCT 
ATTGGTCTCA GAATGAAATT GGGGGACTGG TTCAGAGTAC TGCAGCTCCT GAAAACTGGA 
TCTGGTGATG CAGACGACAG TCTCCTTGAA CAAGCTAACA ATGCCATTGG AGAGTACTTT 
GCTGACCGAC AGAAGTGGCA GAATGCTGTG CAATATTATG TAAAAGGCAG GAACCAGGAG 
CGCCTGGCCG AATGCTATTA TATGCTAGAA GATTATGAGG GGTTAGAGAC TCTTGCCAAT 
TCACTTCCAG AAAACCATAA GTTGCTTCCA GAAATAGCCC AGATGTTTGT GAGAGTTGGA 
ATGTGTGAGC AAGCTGTGAG CGCATTCTTG AAGTGTAACC AACCAAAGGC AGCGGTGGAC 
ACCTGTGTAC ACCTGAACCA ATGGAACAAA GCCGTCGAAC TGGCTAAAAG TCATAGTATG 
AAGGAGATTG GATCTCTGTT AGCTAGGTAC GCGTCCCATT TACTGGAGAA GAACAAGACT 
CTCGATGCCA TTGAACTCTA TAGGAAAGCC AGCTACTTTT TTGATGCAGC TAAACTGATG 
TATAAGATTG CAGATGAAGA GGCGAAGAAA AGGACCAAGC CCCTGCGTGT GAAGAAACTC 
TACGTGCTGT CGGCTCTGCT CATCGAGCAG TATCACGAGC AGATGAAAAA CGCCCAGCGC 
GGCAAAGTTA AAGGCAAGAA CTCAGAGGCC ACTTCTGCTT TGGCTGGCTT GCTAGAAGAG 
GAGGTTCTCT CTACAACTAG TCGGTTCACA GATAACGCCT GGAGAGGAGC GGAGGCCTAC 
CATTTCTTTA TACTCGCGCA GCGGCAGCTC TACGAGGGCT ACGTTGACAC TGCATTGAAG 
ACAGCTCTTC ACCTGAGAGA CTATGAAGAC ATCATTCCCT CAGTTGAGAT CTACTCTCTG 
TTAGCACTCT GTGCGTGTGC AAGCAGAGCT TTTGGTACCT GTTCAAAAGC TTTTATTAAA 
CTGGAATCTT TAGAGACGCT CAGTGCAGAG CAGAAGCAGC AATATGAAGA CCTGGCTCTG 
GAAATCTTCA CCAAACACAC GCCAAAAGAC AACAGGAAGT CTGAATTAAA CAGCCTTCTT 
GAAGGCGGGG AAGGCAAACT GCCGACCTGT ATCGCCACTG GCAGCCCAAT CATCGAGTAT 
CAGTTCTGGG TGTGCAAAGT CTGCAAGCAC TACGTCCTCG CCCAGGAGAT CAGCAACTAC 
AACTTTTGCC CTTTATGCCA CAGCTCGGTA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515295.1
CDS	601..3576
Translation	MKMKLNCLVNVTGAISIAGIHWYHGTEGYVEPDCPCLAICFDNGRCQIMRHENDQNPVLIDTGMYVVGIQWNHIGSVLAVAGSQKVVTQDKDINIVQFYTPFGEHLGTLKVPGKQMCSLSWEGGGLKIALAVDSFIYFANIRPDYKWGYCSNTVVYAYTRPDRPEYCVVFWDTKNSEKYVKYVKSLISITTCGDFCILATKADENHPQEENEMETFGATFVLVLCNSIGTPLDPKYIDLVPLFVAMTKTHVIAASKEAFYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDVPSGSVDGVFDYSKAIQGTRDPICAITASDKTLIVGRESGVIQRYSFPNVALIQKYSLDCRACQLSLNCNSSRLAIIDIAGVLTFFDLDTRVTDSTGQQVVGELLKLERKDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKDPEHPSKDYIMNFEIRSLRDSRALIEKVGIEDASQFIEDNPHPRLWRLLAEAALQKLDLYTAQQAFVRCKDYQGIKFVKLLGNLQSESMKQAEVIAYFGRFEDAERMYQDMDRRDLAIGLRMKLGDWFRVLQLLKTGSGDADDSLLEQANNAIGEYFADRQKWQNAVQYYVKGRNQERLAECYYMLEDYEGLETLANSLPENHKLLPEIAQMFVRVGMCEQAVSAFLKCNQPKAAVDTCVHLNQWNKAVELAKSHSMKEIGSLLARYASHLLEKNKTLDAIELYRKASYFFDAAKLMYKIADEEAKKRTKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKNSEATSALAGLLEEEVLSTTSRFTDNAWRGAEAYHFFILAQRQLYEGYVDTALKTALHLRDYEDIIPSVEIYSLLALCACASRAFGTCSKAFIKLESLETLSAEQKQQYEDLALEIFTKHTPKDNRKSELNSLLEGGEGKLPTCIATGSPIIEYQFWVCKVCKHYVLAQEISNYNFCPLCHSSVE

Target ORF information:

RefSeq Version	XM_006515232.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus WD repeat domain 35 (Wdr35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515232.2

ORF Insert Sequence:

ATGAAAATGA AGCTGAATTG CTTGGTGAAC GTCACTGGAG CGATCAGTAT CGCTGGGATT 
CACTGGTACC ATGGCACCGA AGGCTACGTG GAGCCCGATT GCCCTTGCCT GGCAATTTGC 
TTTGACAATG GGAGATGCCA AATAATGCGG CATGAGAATG ACCAAAACCC GGTTTTGATC 
GACACGGGCA TGTATGTGGT AGGCATCCAG TGGAACCACA TCGGCAGTGT GTTAGCTGTG 
GCCGGCTCCC AGAAGGTAGT CACTCAGGAC AAGGACATCA ACATCGTGCA GTTCTACACA 
CCGTTCGGTG AGCATCTGGG TACTTTGAAA GTTCCAGGAA AGCAGATGTG TTCGCTGTCT 
TGGGAAGGAG GCGGACTGAA GATTGCTCTA GCTGTTGACT CCTTTATATA TTTTGCAAAT 
ATTCGACCTG ATTATAAGTG GGGCTACTGT TCCAACACAG TGGTTTATGC GTACACCAGA 
CCCGATCGTC CCGAATACTG TGTTGTGTTC TGGGACACAA AAAACAGTGA GAAATACGTT 
AAATATGTGA AGAGCCTCAT TTCTATCACC ACGTGTGGAG ATTTCTGCAT TTTGGCTACA 
AAAGCTGATG AGAATCACCC TCAGGAGGAG AATGAAATGG AGACATTTGG TGCAACGTTT 
GTGCTTGTCC TTTGCAATTC TATTGGCACA CCCTTGGACC CTAAATACAT TGATCTTGTA 
CCATTATTTG TTGCAATGAC CAAAACCCAT GTGATAGCAG CTTCAAAAGA AGCATTTTAT 
ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC ACAGCACTGG AAATTAATCA GATCACACGG 
TCTCGAAAAG AAGGAAGAGA AAGAATTTAT CACGTTGATG ATGTCCCTTC CGGATCTGTG 
GATGGGGTGT TTGATTACAG TAAAGCCATT CAAGGTACAA GGGATCCAAT TTGTGCCATT 
ACTGCATCTG ATAAGACATT GATTGTGGGG CGTGAATCTG GCGTCATTCA GAGGTACAGC 
TTTCCTAATG TTGCTCTGAT TCAAAAGTAT TCCCTGGATT GCCGTGCCTG CCAGTTATCT 
CTGAACTGCA ACTCCAGTCG TCTTGCTATC ATCGACATCG CTGGAGTCTT GACTTTCTTT 
GACTTGGACA CTCGGGTGAC CGACAGTACA GGGCAGCAGG TCGTTGGCGA GTTGCTAAAA 
CTGGAGCGCA AAGACGTCTG GGATATGAAG TGGGCCAAGG ACAATCCTGA TTTGTTTGCA 
ATGATGGAGA AGACGAGAAT GTATGTTTTC AGAAACTTGG ATCCTGAGGA ACCCATTCAG 
ACCTCTGGAT ATATTTGCAA CTTTGAGGAT TTGGAAATTA AGTCTGTTCT CTTGGATGAG 
ATACTGAAGG ACCCAGAGCA CCCTAGCAAG GATTACATAA TGAACTTTGA GATCCGGTCC 
CTGCGAGACA GCCGAGCATT GATTGAGAAA GTTGGAATTG AAGATGCGTC TCAGTTCATA 
GAGGACAACC CACACCCCCG ACTTTGGCGC CTGCTGGCTG AAGCTGCTCT CCAGAAGCTG 
GACCTGTACA CCGCACAGCA AGCGTTTGTG CGCTGCAAGG ATTACCAAGG CATCAAGTTT 
GTGAAGCTCC TGGGCAATCT GCAGAGCGAG TCAATGAAGC AGGCTGAAGT TATTGCCTAC 
TTTGGCAGGT TCGAAGATGC CGAAAGGATG TATCAAGATA TGGACAGACG GGACCTCGCT 
ATTGGTCTCA GAATGAAATT GGGGGACTGG TTCAGAGTAC TGCAGCTCCT GAAAACTGGA 
TCTGGTGATG CAGACGACAG TCTCCTTGAA CAAGCTAACA ATGCCATTGG AGAGTACTTT 
GCTGACCGAC AGAAGTGGCA GAATGCTGTG CAATATTATG TAAAAGGCAG GAACCAGGAG 
CGCCTGGCCG AATGCTATTA TATGCTAGAA GATTATGAGG GGTTAGAGAC TCTTGCCAAT 
TCACTTCCAG AAAACCATAA GTTGCTTCCA GAAATAGCCC AGATGTTTGT GAGAGTTGGA 
ATGTGTGAGC AAGCTGTGAG CGCATTCTTG AAGTGTAACC AACCAAAGGC AGCGGTGGAC 
ACCTGTGTAC ACCTGAACCA ATGGAACAAA GCCGTCGAAC TGGCTAAAAG TCATAGTATG 
AAGGAGATTG GATCTCTGTT AGCTAGGTAC GCGTCCCATT TACTGGAGAA GAACAAGACT 
CTCGATGCCA TTGAACTCTA TAGGAAAGCC AGCTACTTTT TTGATGCAGC TAAACTGATG 
TATAAGATTG CAGATGAAGA GGCGAAGAAA AGGACCAAGC CCCTGCGTGT GAAGAAACTC 
TACGTGCTGT CGGCTCTGCT CATCGAGCAG TATCACGAGC AGATGAAAAA CGCCCAGCGC 
GGCAAAGTTA AAGGCAAGAA CTCAGAGGCC ACTTCTGCTT TGGCTGGCTT GCTAGAAGAG 
GAGGTTCTCT CTACAACTAG TCGGTTCACA GATAACGCCT GGAGAGGAGC GGAGGCCTAC 
CATTTCTTTA TACTCGCGCA GCGGCAGCTC TACGAGGGCT ACGTTGACAC TGCATTGAAG 
ACAGCTCTTC ACCTGAGAGA CTATGAAGAC ATCATTCCCT CAGTTGAGAT CTACTCTCTG 
TTAGCACTCT GTGCGTGTGC AAGCAGAGCT TTTGGTACCT GTTCAAAAGC TTTTATTAAA 
CTGGAATCTT TAGAGACGCT CAGTGCAGAG CAGAAGCAGC AATATGAAGA CCTGGCTCTG 
GAAATCTTCA CCAAACACAC GCCAAAAGAC AACAGGAAGT CTGAATTAAA CAGCCTTCTT 
GAAGGCGGGG AAGGCAAACT GCCGACCTGT ATCGCCACTG GCAGCCCAAT CATCGAGTAT 
CAGTTCTGGG TGTGCAAAGT CTGCAAGCAC TACGTCCTCG CCCAGGAGAT CAGCAACTAC 
AACTTTTGCC CTTTATGCCA CAGCTCGGTA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wdr35 ( XM_006515232.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515295.1 Mus musculus WD repeat domain 35 (Wdr35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47424
Clone ID Related Accession (Same CDS sequence)	XM_006515232.2 , XM_006515232.3
Accession Version	XM_006515232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	WD repeat-containing protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515232.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGA AGCTGAATTG CTTGGTGAAC GTCACTGGAG CGATCAGTAT CGCTGGGATT 
CACTGGTACC ATGGCACCGA AGGCTACGTG GAGCCCGATT GCCCTTGCCT GGCAATTTGC 
TTTGACAATG GGAGATGCCA AATAATGCGG CATGAGAATG ACCAAAACCC GGTTTTGATC 
GACACGGGCA TGTATGTGGT AGGCATCCAG TGGAACCACA TCGGCAGTGT GTTAGCTGTG 
GCCGGCTCCC AGAAGGTAGT CACTCAGGAC AAGGACATCA ACATCGTGCA GTTCTACACA 
CCGTTCGGTG AGCATCTGGG TACTTTGAAA GTTCCAGGAA AGCAGATGTG TTCGCTGTCT 
TGGGAAGGAG GCGGACTGAA GATTGCTCTA GCTGTTGACT CCTTTATATA TTTTGCAAAT 
ATTCGACCTG ATTATAAGTG GGGCTACTGT TCCAACACAG TGGTTTATGC GTACACCAGA 
CCCGATCGTC CCGAATACTG TGTTGTGTTC TGGGACACAA AAAACAGTGA GAAATACGTT 
AAATATGTGA AGAGCCTCAT TTCTATCACC ACGTGTGGAG ATTTCTGCAT TTTGGCTACA 
AAAGCTGATG AGAATCACCC TCAGGAGGAG AATGAAATGG AGACATTTGG TGCAACGTTT 
GTGCTTGTCC TTTGCAATTC TATTGGCACA CCCTTGGACC CTAAATACAT TGATCTTGTA 
CCATTATTTG TTGCAATGAC CAAAACCCAT GTGATAGCAG CTTCAAAAGA AGCATTTTAT 
ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC ACAGCACTGG AAATTAATCA GATCACACGG 
TCTCGAAAAG AAGGAAGAGA AAGAATTTAT CACGTTGATG ATGTCCCTTC CGGATCTGTG 
GATGGGGTGT TTGATTACAG TAAAGCCATT CAAGGTACAA GGGATCCAAT TTGTGCCATT 
ACTGCATCTG ATAAGACATT GATTGTGGGG CGTGAATCTG GCGTCATTCA GAGGTACAGC 
TTTCCTAATG TTGCTCTGAT TCAAAAGTAT TCCCTGGATT GCCGTGCCTG CCAGTTATCT 
CTGAACTGCA ACTCCAGTCG TCTTGCTATC ATCGACATCG CTGGAGTCTT GACTTTCTTT 
GACTTGGACA CTCGGGTGAC CGACAGTACA GGGCAGCAGG TCGTTGGCGA GTTGCTAAAA 
CTGGAGCGCA AAGACGTCTG GGATATGAAG TGGGCCAAGG ACAATCCTGA TTTGTTTGCA 
ATGATGGAGA AGACGAGAAT GTATGTTTTC AGAAACTTGG ATCCTGAGGA ACCCATTCAG 
ACCTCTGGAT ATATTTGCAA CTTTGAGGAT TTGGAAATTA AGTCTGTTCT CTTGGATGAG 
ATACTGAAGG ACCCAGAGCA CCCTAGCAAG GATTACATAA TGAACTTTGA GATCCGGTCC 
CTGCGAGACA GCCGAGCATT GATTGAGAAA GTTGGAATTG AAGATGCGTC TCAGTTCATA 
GAGGACAACC CACACCCCCG ACTTTGGCGC CTGCTGGCTG AAGCTGCTCT CCAGAAGCTG 
GACCTGTACA CCGCACAGCA AGCGTTTGTG CGCTGCAAGG ATTACCAAGG CATCAAGTTT 
GTGAAGCTCC TGGGCAATCT GCAGAGCGAG TCAATGAAGC AGGCTGAAGT TATTGCCTAC 
TTTGGCAGGT TCGAAGATGC CGAAAGGATG TATCAAGATA TGGACAGACG GGACCTCGCT 
ATTGGTCTCA GAATGAAATT GGGGGACTGG TTCAGAGTAC TGCAGCTCCT GAAAACTGGA 
TCTGGTGATG CAGACGACAG TCTCCTTGAA CAAGCTAACA ATGCCATTGG AGAGTACTTT 
GCTGACCGAC AGAAGTGGCA GAATGCTGTG CAATATTATG TAAAAGGCAG GAACCAGGAG 
CGCCTGGCCG AATGCTATTA TATGCTAGAA GATTATGAGG GGTTAGAGAC TCTTGCCAAT 
TCACTTCCAG AAAACCATAA GTTGCTTCCA GAAATAGCCC AGATGTTTGT GAGAGTTGGA 
ATGTGTGAGC AAGCTGTGAG CGCATTCTTG AAGTGTAACC AACCAAAGGC AGCGGTGGAC 
ACCTGTGTAC ACCTGAACCA ATGGAACAAA GCCGTCGAAC TGGCTAAAAG TCATAGTATG 
AAGGAGATTG GATCTCTGTT AGCTAGGTAC GCGTCCCATT TACTGGAGAA GAACAAGACT 
CTCGATGCCA TTGAACTCTA TAGGAAAGCC AGCTACTTTT TTGATGCAGC TAAACTGATG 
TATAAGATTG CAGATGAAGA GGCGAAGAAA AGGACCAAGC CCCTGCGTGT GAAGAAACTC 
TACGTGCTGT CGGCTCTGCT CATCGAGCAG TATCACGAGC AGATGAAAAA CGCCCAGCGC 
GGCAAAGTTA AAGGCAAGAA CTCAGAGGCC ACTTCTGCTT TGGCTGGCTT GCTAGAAGAG 
GAGGTTCTCT CTACAACTAG TCGGTTCACA GATAACGCCT GGAGAGGAGC GGAGGCCTAC 
CATTTCTTTA TACTCGCGCA GCGGCAGCTC TACGAGGGCT ACGTTGACAC TGCATTGAAG 
ACAGCTCTTC ACCTGAGAGA CTATGAAGAC ATCATTCCCT CAGTTGAGAT CTACTCTCTG 
TTAGCACTCT GTGCGTGTGC AAGCAGAGCT TTTGGTACCT GTTCAAAAGC TTTTATTAAA 
CTGGAATCTT TAGAGACGCT CAGTGCAGAG CAGAAGCAGC AATATGAAGA CCTGGCTCTG 
GAAATCTTCA CCAAACACAC GCCAAAAGAC AACAGGAAGT CTGAATTAAA CAGCCTTCTT 
GAAGGCGGGG AAGGCAAACT GCCGACCTGT ATCGCCACTG GCAGCCCAAT CATCGAGTAT 
CAGTTCTGGG TGTGCAAAGT CTGCAAGCAC TACGTCCTCG CCCAGGAGAT CAGCAACTAC 
AACTTTTGCC CTTTATGCCA CAGCTCGGTA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515295.1
CDS	595..3570
Translation	MKMKLNCLVNVTGAISIAGIHWYHGTEGYVEPDCPCLAICFDNGRCQIMRHENDQNPVLIDTGMYVVGIQWNHIGSVLAVAGSQKVVTQDKDINIVQFYTPFGEHLGTLKVPGKQMCSLSWEGGGLKIALAVDSFIYFANIRPDYKWGYCSNTVVYAYTRPDRPEYCVVFWDTKNSEKYVKYVKSLISITTCGDFCILATKADENHPQEENEMETFGATFVLVLCNSIGTPLDPKYIDLVPLFVAMTKTHVIAASKEAFYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDVPSGSVDGVFDYSKAIQGTRDPICAITASDKTLIVGRESGVIQRYSFPNVALIQKYSLDCRACQLSLNCNSSRLAIIDIAGVLTFFDLDTRVTDSTGQQVVGELLKLERKDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKDPEHPSKDYIMNFEIRSLRDSRALIEKVGIEDASQFIEDNPHPRLWRLLAEAALQKLDLYTAQQAFVRCKDYQGIKFVKLLGNLQSESMKQAEVIAYFGRFEDAERMYQDMDRRDLAIGLRMKLGDWFRVLQLLKTGSGDADDSLLEQANNAIGEYFADRQKWQNAVQYYVKGRNQERLAECYYMLEDYEGLETLANSLPENHKLLPEIAQMFVRVGMCEQAVSAFLKCNQPKAAVDTCVHLNQWNKAVELAKSHSMKEIGSLLARYASHLLEKNKTLDAIELYRKASYFFDAAKLMYKIADEEAKKRTKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKNSEATSALAGLLEEEVLSTTSRFTDNAWRGAEAYHFFILAQRQLYEGYVDTALKTALHLRDYEDIIPSVEIYSLLALCACASRAFGTCSKAFIKLESLETLSAEQKQQYEDLALEIFTKHTPKDNRKSELNSLLEGGEGKLPTCIATGSPIIEYQFWVCKVCKHYVLAQEISNYNFCPLCHSSVE

Target ORF information:

RefSeq Version	XM_006515232.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WD repeat domain 35 (Wdr35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515232.3

ORF Insert Sequence:

ATGAAAATGA AGCTGAATTG CTTGGTGAAC GTCACTGGAG CGATCAGTAT CGCTGGGATT 
CACTGGTACC ATGGCACCGA AGGCTACGTG GAGCCCGATT GCCCTTGCCT GGCAATTTGC 
TTTGACAATG GGAGATGCCA AATAATGCGG CATGAGAATG ACCAAAACCC GGTTTTGATC 
GACACGGGCA TGTATGTGGT AGGCATCCAG TGGAACCACA TCGGCAGTGT GTTAGCTGTG 
GCCGGCTCCC AGAAGGTAGT CACTCAGGAC AAGGACATCA ACATCGTGCA GTTCTACACA 
CCGTTCGGTG AGCATCTGGG TACTTTGAAA GTTCCAGGAA AGCAGATGTG TTCGCTGTCT 
TGGGAAGGAG GCGGACTGAA GATTGCTCTA GCTGTTGACT CCTTTATATA TTTTGCAAAT 
ATTCGACCTG ATTATAAGTG GGGCTACTGT TCCAACACAG TGGTTTATGC GTACACCAGA 
CCCGATCGTC CCGAATACTG TGTTGTGTTC TGGGACACAA AAAACAGTGA GAAATACGTT 
AAATATGTGA AGAGCCTCAT TTCTATCACC ACGTGTGGAG ATTTCTGCAT TTTGGCTACA 
AAAGCTGATG AGAATCACCC TCAGGAGGAG AATGAAATGG AGACATTTGG TGCAACGTTT 
GTGCTTGTCC TTTGCAATTC TATTGGCACA CCCTTGGACC CTAAATACAT TGATCTTGTA 
CCATTATTTG TTGCAATGAC CAAAACCCAT GTGATAGCAG CTTCAAAAGA AGCATTTTAT 
ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC ACAGCACTGG AAATTAATCA GATCACACGG 
TCTCGAAAAG AAGGAAGAGA AAGAATTTAT CACGTTGATG ATGTCCCTTC CGGATCTGTG 
GATGGGGTGT TTGATTACAG TAAAGCCATT CAAGGTACAA GGGATCCAAT TTGTGCCATT 
ACTGCATCTG ATAAGACATT GATTGTGGGG CGTGAATCTG GCGTCATTCA GAGGTACAGC 
TTTCCTAATG TTGCTCTGAT TCAAAAGTAT TCCCTGGATT GCCGTGCCTG CCAGTTATCT 
CTGAACTGCA ACTCCAGTCG TCTTGCTATC ATCGACATCG CTGGAGTCTT GACTTTCTTT 
GACTTGGACA CTCGGGTGAC CGACAGTACA GGGCAGCAGG TCGTTGGCGA GTTGCTAAAA 
CTGGAGCGCA AAGACGTCTG GGATATGAAG TGGGCCAAGG ACAATCCTGA TTTGTTTGCA 
ATGATGGAGA AGACGAGAAT GTATGTTTTC AGAAACTTGG ATCCTGAGGA ACCCATTCAG 
ACCTCTGGAT ATATTTGCAA CTTTGAGGAT TTGGAAATTA AGTCTGTTCT CTTGGATGAG 
ATACTGAAGG ACCCAGAGCA CCCTAGCAAG GATTACATAA TGAACTTTGA GATCCGGTCC 
CTGCGAGACA GCCGAGCATT GATTGAGAAA GTTGGAATTG AAGATGCGTC TCAGTTCATA 
GAGGACAACC CACACCCCCG ACTTTGGCGC CTGCTGGCTG AAGCTGCTCT CCAGAAGCTG 
GACCTGTACA CCGCACAGCA AGCGTTTGTG CGCTGCAAGG ATTACCAAGG CATCAAGTTT 
GTGAAGCTCC TGGGCAATCT GCAGAGCGAG TCAATGAAGC AGGCTGAAGT TATTGCCTAC 
TTTGGCAGGT TCGAAGATGC CGAAAGGATG TATCAAGATA TGGACAGACG GGACCTCGCT 
ATTGGTCTCA GAATGAAATT GGGGGACTGG TTCAGAGTAC TGCAGCTCCT GAAAACTGGA 
TCTGGTGATG CAGACGACAG TCTCCTTGAA CAAGCTAACA ATGCCATTGG AGAGTACTTT 
GCTGACCGAC AGAAGTGGCA GAATGCTGTG CAATATTATG TAAAAGGCAG GAACCAGGAG 
CGCCTGGCCG AATGCTATTA TATGCTAGAA GATTATGAGG GGTTAGAGAC TCTTGCCAAT 
TCACTTCCAG AAAACCATAA GTTGCTTCCA GAAATAGCCC AGATGTTTGT GAGAGTTGGA 
ATGTGTGAGC AAGCTGTGAG CGCATTCTTG AAGTGTAACC AACCAAAGGC AGCGGTGGAC 
ACCTGTGTAC ACCTGAACCA ATGGAACAAA GCCGTCGAAC TGGCTAAAAG TCATAGTATG 
AAGGAGATTG GATCTCTGTT AGCTAGGTAC GCGTCCCATT TACTGGAGAA GAACAAGACT 
CTCGATGCCA TTGAACTCTA TAGGAAAGCC AGCTACTTTT TTGATGCAGC TAAACTGATG 
TATAAGATTG CAGATGAAGA GGCGAAGAAA AGGACCAAGC CCCTGCGTGT GAAGAAACTC 
TACGTGCTGT CGGCTCTGCT CATCGAGCAG TATCACGAGC AGATGAAAAA CGCCCAGCGC 
GGCAAAGTTA AAGGCAAGAA CTCAGAGGCC ACTTCTGCTT TGGCTGGCTT GCTAGAAGAG 
GAGGTTCTCT CTACAACTAG TCGGTTCACA GATAACGCCT GGAGAGGAGC GGAGGCCTAC 
CATTTCTTTA TACTCGCGCA GCGGCAGCTC TACGAGGGCT ACGTTGACAC TGCATTGAAG 
ACAGCTCTTC ACCTGAGAGA CTATGAAGAC ATCATTCCCT CAGTTGAGAT CTACTCTCTG 
TTAGCACTCT GTGCGTGTGC AAGCAGAGCT TTTGGTACCT GTTCAAAAGC TTTTATTAAA 
CTGGAATCTT TAGAGACGCT CAGTGCAGAG CAGAAGCAGC AATATGAAGA CCTGGCTCTG 
GAAATCTTCA CCAAACACAC GCCAAAAGAC AACAGGAAGT CTGAATTAAA CAGCCTTCTT 
GAAGGCGGGG AAGGCAAACT GCCGACCTGT ATCGCCACTG GCAGCCCAAT CATCGAGTAT 
CAGTTCTGGG TGTGCAAAGT CTGCAAGCAC TACGTCCTCG CCCAGGAGAT CAGCAACTAC 
AACTTTTGCC CTTTATGCCA CAGCTCGGTA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wdr35 ( NM_172470.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766058.3 Mus musculus WD repeat domain 35 (Wdr35), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22548
Clone ID Related Accession (Same CDS sequence)	NM_172470.3
Accession Version	NM_172470.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	WD repeat-containing protein 35 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC056925.1 and AK029896.1. On Apr 18, 2009 this sequence version replaced NM_172470.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056925.1, SRR7974084.17866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN00849390 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTTCT ACCTGAGCAA GAAAATTGCT GTTCCCAATA ATGTGAAGCT GAAATGTATA 
TCCTGGAACA AGGACCAAGG GTTCATAGCA TGTGGCGGTG AGGATGGATT ACTGAAAGTT 
TTAAGACTCG AGACACAGAC AGATGACTCA AAGCTGAGGG GTCTAGCCGC CCCTAGTAAC 
CTTTCCATGA ACCAAAATCT TGAAGGTCAT AGTGGTGCTG TTCAAGTTGT AACGTGGAAT 
GAACAGTATC AGAAGTTGAC TACCAGTGAT CAAAATGGGC TTATCATTGT CTGGATGCTG 
TATAAAGGCT CTTGGTATGA GGAGATGATC AACAATCGCA ACAAGTCTGT GGTTCGAAGC 
ATGAGCTGGA ATGCGGACGG ACAGAAGATC TGCATTGTGT ACGAAGATGG AGCTGTGATC 
GTCGGCTCAG TAGACGGGAA TCGGATTTGG GGGAAAGACC TGAAGGGAAT TCAGCTGTGC 
CATGTGACCT GGTCTGCAGA TAGTAAAATC CTACTTTTTG GAATGGCAAA TGGAGAAATA 
CACATTTATG ATAATCAAGG AAATTTTATA ATGAAAATGA AGCTGAATTG CTTGGTGAAC 
GTCACTGGAG CGATCAGTAT CGCTGGGATT CACTGGTACC ATGGCACCGA AGGCTACGTG 
GAGCCCGATT GCCCTTGCCT GGCAATTTGC TTTGACAATG GGAGATGCCA AATAATGCGG 
CATGAGAATG ACCAAAACCC GGTTTTGATC GACACGGGCA TGTATGTGGT AGGCATCCAG 
TGGAACCACA TCGGCAGTGT GTTAGCTGTG GCCGGCTCCC AGAAGGTAGT CACTCAGGAC 
AAGGACATCA ACATCGTGCA GTTCTACACA CCGTTCGGTG AGCATCTGGG TACTTTGAAA 
GTTCCAGGAA AGCAGATGTG TTCGCTGTCT TGGGAAGGAG GCGGACTGAA GATTGCTCTA 
GCTGTTGACT CCTTTATATA TTTTGCAAAT ATTCGACCTG ATTATAAGTG GGGCTACTGT 
TCCAACACAG TGGTTTATGC GTACACCAGA CCCGATCGTC CCGAATACTG TGTTGTGTTC 
TGGGACACAA AAAACAGTGA GAAATACGTT AAATATGTGA AGAGCCTCAT TTCTATCACC 
ACGTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AGAATCACCC TCAGGAGGAG 
AATGAAATGG AGACATTTGG TGCAACGTTT GTGCTTGTCC TTTGCAATTC TATTGGCACA 
CCCTTGGACC CTAAATACAT TGATCTTGTA CCATTATTTG TTGCAATGAC CAAAACCCAT 
GTGATAGCAG CTTCAAAAGA AGCATTTTAT ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC 
ACAGCACTGG AAATTAATCA GATCACACGG TCTCGAAAAG AAGGAAGAGA AAGAATTTAT 
CACGTTGATG ATGTCCCTTC CGGATCTGTG GATGGGGTGT TTGATTACAG TAAAGCCATT 
CAAGGTACAA GGGATCCAAT TTGTGCCATT ACTGCATCTG ATAAGACATT GATTGTGGGG 
CGTGAATCTG GCGTCATTCA GAGGTACAGC TTTCCTAATG TTGCTCTGAT TCAAAAGTAT 
TCCCTGGATT GCCGTGCCTG CCAGTTATCT CTGAACTGCA ACTCCAGTCG TCTTGCTATC 
ATCGACATCG CTGGAGTCTT GACTTTCTTT GACTTGGACA CTCGGGTGAC CGACAGTACA 
GGGCAGCAGG TCGTTGGCGA GTTGCTAAAA CTGGAGCGCA AAGACGTCTG GGATATGAAG 
TGGGCCAAGG ACAATCCTGA TTTGTTTGCA ATGATGGAGA AGACGAGAAT GTATGTTTTC 
AGAAACTTGG ATCCTGAGGA ACCCATTCAG ACCTCTGGAT ATATTTGCAA CTTTGAGGAT 
TTGGAAATTA AGTCTGTTCT CTTGGATGAG ATACTGAAGG ACCCAGAGCA CCCTAGCAAG 
GATTACATAA TGAACTTTGA GATCCGGTCC CTGCGAGACA GCCGAGCATT GATTGAGAAA 
GTTGGAATTG AAGATGCGTC TCAGTTCATA GAGGACAACC CACACCCCCG ACTTTGGCGC 
CTGCTGGCTG AAGCTGCTCT CCAGAAGCTG GACCTGTACA CCGCACAGCA AGCGTTTGTG 
CGCTGCAAGG ATTACCAAGG CATCAAGTTT GTGAAGCTCC TGGGCAATCT GCAGAGCGAG 
TCAATGAAGC AGGCTGAAGT TATTGCCTAC TTTGGCAGGT TCGAAGATGC CGAAAGGATG 
TATCAAGATA TGGACAGACG GGACCTCGCT ATTGGTCTCA GAATGAAATT GGGGGACTGG 
TTCAGAGTAC TGCAGCTCCT GAAAACTGGA TCTGGTGATG CAGACGACAG TCTCCTTGAA 
CAAGCTAACA ATGCCATTGG AGAGTACTTT GCTGACCGAC AGAAGTGGCA GAATGCTGTG 
CAATATTATG TAAAAGGCAG GAACCAGGAG CGCCTGGCCG AATGCTATTA TATGCTAGAA 
GATTATGAGG GGTTAGAGAC TCTTGCCAAT TCACTTCCAG AAAACCATAA GTTGCTTCCA 
GAAATAGCCC AGATGTTTGT GAGAGTTGGA ATGTGTGAGC AAGCTGTGAG CGCATTCTTG 
AAGTGTAACC AACCAAAGGC AGCGGTGGAC ACCTGTGTAC ACCTGAACCA ATGGAACAAA 
GCCGTCGAAC TGGCTAAAAG TCATAGTATG AAGGAGATTG GATCTCTGTT AGCTAGGTAC 
GCGTCCCATT TACTGGAGAA GAACAAGACT CTCGATGCCA TTGAACTCTA TAGGAAAGCC 
AGCTACTTTT TTGATGCAGC TAAACTGATG TATAAGATTG CAGATGAAGA GGCGAAGAAA 
AGGACCAAGC CCCTGCGTGT GAAGAAACTC TACGTGCTGT CGGCTCTGCT CATCGAGCAG 
TATCACGAGC AGATGAAAAA CGCCCAGCGC GGCAAAGTTA AAGGCAAGAA CTCAGAGGCC 
ACTTCTGCTT TGGCTGGCTT GCTAGAAGAG GAGGTTCTCT CTACAACTAG TCGGTTCACA 
GATAACGCCT GGAGAGGAGC GGAGGCCTAC CATTTCTTTA TACTCGCGCA GCGGCAGCTC 
TACGAGGGCT ACGTTGACAC TGCATTGAAG ACAGCTCTTC ACCTGAGAGA CTATGAAGAC 
ATCATTCCCT CAGTTGAGAT CTACTCTCTG TTAGCACTCT GTGCGTGTGC AAGCAGAGCT 
TTTGGTACCT GTTCAAAAGC TTTTATTAAA CTGGAATCTT TAGAGACGCT CAGTGCAGAG 
CAGAAGCAGC AATATGAAGA CCTGGCTCTG GAAATCTTCA CCAAACACAC GCCAAAAGAC 
AACAGGAAGT CTGAATTAAA CAGCCTTCTT GAAGGCGGGG AAGGCAAACT GCCGACCTGT 
ATCGCCACTG GCAGCCCAAT CATCGAGTAT CAGTTCTGGG TGTGCAAAGT CTGCAAGCAC 
TACGTCCTCG CCCAGGAGAT CAGCAACTAC AACTTTTGCC CTTTATGCCA CAGCTCGGTA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766058.3
CDS	156..3701
Translation	MFFYLSKKIAVPNNVKLKCISWNKDQGFIACGGEDGLLKVLRLETQTDDSKLRGLAAPSNLSMNQNLEGHSGAVQVVTWNEQYQKLTTSDQNGLIIVWMLYKGSWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGKDLKGIQLCHVTWSADSKILLFGMANGEIHIYDNQGNFIMKMKLNCLVNVTGAISIAGIHWYHGTEGYVEPDCPCLAICFDNGRCQIMRHENDQNPVLIDTGMYVVGIQWNHIGSVLAVAGSQKVVTQDKDINIVQFYTPFGEHLGTLKVPGKQMCSLSWEGGGLKIALAVDSFIYFANIRPDYKWGYCSNTVVYAYTRPDRPEYCVVFWDTKNSEKYVKYVKSLISITTCGDFCILATKADENHPQEENEMETFGATFVLVLCNSIGTPLDPKYIDLVPLFVAMTKTHVIAASKEAFYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDVPSGSVDGVFDYSKAIQGTRDPICAITASDKTLIVGRESGVIQRYSFPNVALIQKYSLDCRACQLSLNCNSSRLAIIDIAGVLTFFDLDTRVTDSTGQQVVGELLKLERKDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKDPEHPSKDYIMNFEIRSLRDSRALIEKVGIEDASQFIEDNPHPRLWRLLAEAALQKLDLYTAQQAFVRCKDYQGIKFVKLLGNLQSESMKQAEVIAYFGRFEDAERMYQDMDRRDLAIGLRMKLGDWFRVLQLLKTGSGDADDSLLEQANNAIGEYFADRQKWQNAVQYYVKGRNQERLAECYYMLEDYEGLETLANSLPENHKLLPEIAQMFVRVGMCEQAVSAFLKCNQPKAAVDTCVHLNQWNKAVELAKSHSMKEIGSLLARYASHLLEKNKTLDAIELYRKASYFFDAAKLMYKIADEEAKKRTKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKNSEATSALAGLLEEEVLSTTSRFTDNAWRGAEAYHFFILAQRQLYEGYVDTALKTALHLRDYEDIIPSVEIYSLLALCACASRAFGTCSKAFIKLESLETLSAEQKQQYEDLALEIFTKHTPKDNRKSELNSLLEGGEGKLPTCIATGSPIIEYQFWVCKVCKHYVLAQEISNYNFCPLCHSSVE

Target ORF information:

RefSeq Version	NM_172470.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WD repeat domain 35 (Wdr35), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172470.3

ORF Insert Sequence:

ATGTTCTTCT ACCTGAGCAA GAAAATTGCT GTTCCCAATA ATGTGAAGCT GAAATGTATA 
TCCTGGAACA AGGACCAAGG GTTCATAGCA TGTGGCGGTG AGGATGGATT ACTGAAAGTT 
TTAAGACTCG AGACACAGAC AGATGACTCA AAGCTGAGGG GTCTAGCCGC CCCTAGTAAC 
CTTTCCATGA ACCAAAATCT TGAAGGTCAT AGTGGTGCTG TTCAAGTTGT AACGTGGAAT 
GAACAGTATC AGAAGTTGAC TACCAGTGAT CAAAATGGGC TTATCATTGT CTGGATGCTG 
TATAAAGGCT CTTGGTATGA GGAGATGATC AACAATCGCA ACAAGTCTGT GGTTCGAAGC 
ATGAGCTGGA ATGCGGACGG ACAGAAGATC TGCATTGTGT ACGAAGATGG AGCTGTGATC 
GTCGGCTCAG TAGACGGGAA TCGGATTTGG GGGAAAGACC TGAAGGGAAT TCAGCTGTGC 
CATGTGACCT GGTCTGCAGA TAGTAAAATC CTACTTTTTG GAATGGCAAA TGGAGAAATA 
CACATTTATG ATAATCAAGG AAATTTTATA ATGAAAATGA AGCTGAATTG CTTGGTGAAC 
GTCACTGGAG CGATCAGTAT CGCTGGGATT CACTGGTACC ATGGCACCGA AGGCTACGTG 
GAGCCCGATT GCCCTTGCCT GGCAATTTGC TTTGACAATG GGAGATGCCA AATAATGCGG 
CATGAGAATG ACCAAAACCC GGTTTTGATC GACACGGGCA TGTATGTGGT AGGCATCCAG 
TGGAACCACA TCGGCAGTGT GTTAGCTGTG GCCGGCTCCC AGAAGGTAGT CACTCAGGAC 
AAGGACATCA ACATCGTGCA GTTCTACACA CCGTTCGGTG AGCATCTGGG TACTTTGAAA 
GTTCCAGGAA AGCAGATGTG TTCGCTGTCT TGGGAAGGAG GCGGACTGAA GATTGCTCTA 
GCTGTTGACT CCTTTATATA TTTTGCAAAT ATTCGACCTG ATTATAAGTG GGGCTACTGT 
TCCAACACAG TGGTTTATGC GTACACCAGA CCCGATCGTC CCGAATACTG TGTTGTGTTC 
TGGGACACAA AAAACAGTGA GAAATACGTT AAATATGTGA AGAGCCTCAT TTCTATCACC 
ACGTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AGAATCACCC TCAGGAGGAG 
AATGAAATGG AGACATTTGG TGCAACGTTT GTGCTTGTCC TTTGCAATTC TATTGGCACA 
CCCTTGGACC CTAAATACAT TGATCTTGTA CCATTATTTG TTGCAATGAC CAAAACCCAT 
GTGATAGCAG CTTCAAAAGA AGCATTTTAT ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC 
ACAGCACTGG AAATTAATCA GATCACACGG TCTCGAAAAG AAGGAAGAGA AAGAATTTAT 
CACGTTGATG ATGTCCCTTC CGGATCTGTG GATGGGGTGT TTGATTACAG TAAAGCCATT 
CAAGGTACAA GGGATCCAAT TTGTGCCATT ACTGCATCTG ATAAGACATT GATTGTGGGG 
CGTGAATCTG GCGTCATTCA GAGGTACAGC TTTCCTAATG TTGCTCTGAT TCAAAAGTAT 
TCCCTGGATT GCCGTGCCTG CCAGTTATCT CTGAACTGCA ACTCCAGTCG TCTTGCTATC 
ATCGACATCG CTGGAGTCTT GACTTTCTTT GACTTGGACA CTCGGGTGAC CGACAGTACA 
GGGCAGCAGG TCGTTGGCGA GTTGCTAAAA CTGGAGCGCA AAGACGTCTG GGATATGAAG 
TGGGCCAAGG ACAATCCTGA TTTGTTTGCA ATGATGGAGA AGACGAGAAT GTATGTTTTC 
AGAAACTTGG ATCCTGAGGA ACCCATTCAG ACCTCTGGAT ATATTTGCAA CTTTGAGGAT 
TTGGAAATTA AGTCTGTTCT CTTGGATGAG ATACTGAAGG ACCCAGAGCA CCCTAGCAAG 
GATTACATAA TGAACTTTGA GATCCGGTCC CTGCGAGACA GCCGAGCATT GATTGAGAAA 
GTTGGAATTG AAGATGCGTC TCAGTTCATA GAGGACAACC CACACCCCCG ACTTTGGCGC 
CTGCTGGCTG AAGCTGCTCT CCAGAAGCTG GACCTGTACA CCGCACAGCA AGCGTTTGTG 
CGCTGCAAGG ATTACCAAGG CATCAAGTTT GTGAAGCTCC TGGGCAATCT GCAGAGCGAG 
TCAATGAAGC AGGCTGAAGT TATTGCCTAC TTTGGCAGGT TCGAAGATGC CGAAAGGATG 
TATCAAGATA TGGACAGACG GGACCTCGCT ATTGGTCTCA GAATGAAATT GGGGGACTGG 
TTCAGAGTAC TGCAGCTCCT GAAAACTGGA TCTGGTGATG CAGACGACAG TCTCCTTGAA 
CAAGCTAACA ATGCCATTGG AGAGTACTTT GCTGACCGAC AGAAGTGGCA GAATGCTGTG 
CAATATTATG TAAAAGGCAG GAACCAGGAG CGCCTGGCCG AATGCTATTA TATGCTAGAA 
GATTATGAGG GGTTAGAGAC TCTTGCCAAT TCACTTCCAG AAAACCATAA GTTGCTTCCA 
GAAATAGCCC AGATGTTTGT GAGAGTTGGA ATGTGTGAGC AAGCTGTGAG CGCATTCTTG 
AAGTGTAACC AACCAAAGGC AGCGGTGGAC ACCTGTGTAC ACCTGAACCA ATGGAACAAA 
GCCGTCGAAC TGGCTAAAAG TCATAGTATG AAGGAGATTG GATCTCTGTT AGCTAGGTAC 
GCGTCCCATT TACTGGAGAA GAACAAGACT CTCGATGCCA TTGAACTCTA TAGGAAAGCC 
AGCTACTTTT TTGATGCAGC TAAACTGATG TATAAGATTG CAGATGAAGA GGCGAAGAAA 
AGGACCAAGC CCCTGCGTGT GAAGAAACTC TACGTGCTGT CGGCTCTGCT CATCGAGCAG 
TATCACGAGC AGATGAAAAA CGCCCAGCGC GGCAAAGTTA AAGGCAAGAA CTCAGAGGCC 
ACTTCTGCTT TGGCTGGCTT GCTAGAAGAG GAGGTTCTCT CTACAACTAG TCGGTTCACA 
GATAACGCCT GGAGAGGAGC GGAGGCCTAC CATTTCTTTA TACTCGCGCA GCGGCAGCTC 
TACGAGGGCT ACGTTGACAC TGCATTGAAG ACAGCTCTTC ACCTGAGAGA CTATGAAGAC 
ATCATTCCCT CAGTTGAGAT CTACTCTCTG TTAGCACTCT GTGCGTGTGC AAGCAGAGCT 
TTTGGTACCT GTTCAAAAGC TTTTATTAAA CTGGAATCTT TAGAGACGCT CAGTGCAGAG 
CAGAAGCAGC AATATGAAGA CCTGGCTCTG GAAATCTTCA CCAAACACAC GCCAAAAGAC 
AACAGGAAGT CTGAATTAAA CAGCCTTCTT GAAGGCGGGG AAGGCAAACT GCCGACCTGT 
ATCGCCACTG GCAGCCCAAT CATCGAGTAT CAGTTCTGGG TGTGCAAAGT CTGCAAGCAC 
TACGTCCTCG CCCAGGAGAT CAGCAACTAC AACTTTTGCC CTTTATGCCA CAGCTCGGTA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wdr35 ( NM_001159527.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152999.1 Mus musculus WD repeat domain 35 (Wdr35), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22587
Clone ID Related Accession (Same CDS sequence)	NM_001159527.1
Accession Version	NM_001159527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	WD repeat-containing protein 35 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC056925.1 and AK029896.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform (2) thus lacks an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029896.1, AK083971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTTCT ACCTGAGCAA GAAAATTGCT GTTCCCAATA ATGTGAAGCT GAAATGTATA 
TCCTGGAACA AGGACCAAGG GTTCATAGCA TGTGGCGGTG AGGATGGATT ACTGAAAGTT 
TTAAGACTCG AGACACAGAC AGATGACTCA AAGCTGAGGG GTCTAGCCGC CCCTAGTAAC 
CTTTCCATGA ACCAAAATCT TGAAGGTCAT AGTGGTGCTG TTCAAGTTGT AACGTGGAAT 
GAACAGTATC AGAAGTTGAC TACCAGTGAT CAAAATGGGC TTATCATTGT CTGGATGCTG 
TATAAAGGCT CTTGGTATGA GGAGATGATC AACAATCGCA ACAAGTCTGT GGTTCGAAGC 
ATGAGCTGGA ATGCGGACGG ACAGAAGATC TGCATTGTGT ACGAAGATGG AGCTGTGATC 
GTCGGCTCAG TAGACGGGAA TCGGATTTGG GGGAAAGACC TGAAGGGAAT TCAGCTGTGC 
CATGTGACCT GGTCTGCAGA TAGTAAAATC CTACTTTTTG GAATGGCAAA TGGAGAAATA 
CACATTTATG ATAATCAAGG AAATTTTATA ATGAAAATGA AGCTGAATTG CTTGGTGAAC 
GTCACTGGAG CGATCAGTAT CGCTGGGATT CACTGGTACC ATGGCACCGA AGGCTACGTG 
GAGCCCGATT GCCCTTGCCT GGCAATTTGC TTTGACAATG GGAGATGCCA AATAATGCGG 
CATGAGAATG ACCAAAACCC GGTTTTGATC GACACGGGCA TGTATGTGGT AGGCATCCAG 
TGGAACCACA TCGGCAGTGT GTTAGCTGTG GCCGGCTCCC AGAAGGTAGT CACTCAGGAC 
AAGGACATCA ACATCGTGCA GTTCTACACA CCGTTCGGTG AGCATCTGGG TACTTTGAAA 
GTTCCAGGAA AGCAGATGTG TTCGCTGTCT TGGGAAGGAG GCGGACTGAA GATTGCTCTA 
GCTGTTGACT CCTTTATATA TTTTGCAAAT ATTCGACCTG ATTATAAGTG GGGCTACTGT 
TCCAACACAG TGGTTTATGC GTACACCAGA CCCGATCGTC CCGAATACTG TGTTGTGTTC 
TGGGACACAA AAAACAGTGA GAAATACGTT AAATATGTGA AGAGCCTCAT TTCTATCACC 
ACGTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AGAATCACCC TCAGTTTGTG 
CTTGTCCTTT GCAATTCTAT TGGCACACCC TTGGACCCTA AATACATTGA TCTTGTACCA 
TTATTTGTTG CAATGACCAA AACCCATGTG ATAGCAGCTT CAAAAGAAGC ATTTTATACC 
TGGCAATATC GTGTGGCAAA GAAGCTCACA GCACTGGAAA TTAATCAGAT CACACGGTCT 
CGAAAAGAAG GAAGAGAAAG AATTTATCAC GTTGATGATG TCCCTTCCGG ATCTGTGGAT 
GGGGTGTTTG ATTACAGTAA AGCCATTCAA GGTACAAGGG ATCCAATTTG TGCCATTACT 
GCATCTGATA AGACATTGAT TGTGGGGCGT GAATCTGGCG TCATTCAGAG GTACAGCTTT 
CCTAATGTTG CTCTGATTCA AAAGTATTCC CTGGATTGCC GTGCCTGCCA GTTATCTCTG 
AACTGCAACT CCAGTCGTCT TGCTATCATC GACATCGCTG GAGTCTTGAC TTTCTTTGAC 
TTGGACACTC GGGTGACCGA CAGTACAGGG CAGCAGGTCG TTGGCGAGTT GCTAAAACTG 
GAGCGCAAAG ACGTCTGGGA TATGAAGTGG GCCAAGGACA ATCCTGATTT GTTTGCAATG 
ATGGAGAAGA CGAGAATGTA TGTTTTCAGA AACTTGGATC CTGAGGAACC CATTCAGACC 
TCTGGATATA TTTGCAACTT TGAGGATTTG GAAATTAAGT CTGTTCTCTT GGATGAGATA 
CTGAAGGACC CAGAGCACCC TAGCAAGGAT TACATAATGA ACTTTGAGAT CCGGTCCCTG 
CGAGACAGCC GAGCATTGAT TGAGAAAGTT GGAATTGAAG ATGCGTCTCA GTTCATAGAG 
GACAACCCAC ACCCCCGACT TTGGCGCCTG CTGGCTGAAG CTGCTCTCCA GAAGCTGGAC 
CTGTACACCG CACAGCAAGC GTTTGTGCGC TGCAAGGATT ACCAAGGCAT CAAGTTTGTG 
AAGCTCCTGG GCAATCTGCA GAGCGAGTCA ATGAAGCAGG CTGAAGTTAT TGCCTACTTT 
GGCAGGTTCG AAGATGCCGA AAGGATGTAT CAAGATATGG ACAGACGGGA CCTCGCTATT 
GGTCTCAGAA TGAAATTGGG GGACTGGTTC AGAGTACTGC AGCTCCTGAA AACTGGATCT 
GGTGATGCAG ACGACAGTCT CCTTGAACAA GCTAACAATG CCATTGGAGA GTACTTTGCT 
GACCGACAGA AGTGGCAGAA TGCTGTGCAA TATTATGTAA AAGGCAGGAA CCAGGAGCGC 
CTGGCCGAAT GCTATTATAT GCTAGAAGAT TATGAGGGGT TAGAGACTCT TGCCAATTCA 
CTTCCAGAAA ACCATAAGTT GCTTCCAGAA ATAGCCCAGA TGTTTGTGAG AGTTGGAATG 
TGTGAGCAAG CTGTGAGCGC ATTCTTGAAG TGTAACCAAC CAAAGGCAGC GGTGGACACC 
TGTGTACACC TGAACCAATG GAACAAAGCC GTCGAACTGG CTAAAAGTCA TAGTATGAAG 
GAGATTGGAT CTCTGTTAGC TAGGTACGCG TCCCATTTAC TGGAGAAGAA CAAGACTCTC 
GATGCCATTG AACTCTATAG GAAAGCCAGC TACTTTTTTG ATGCAGCTAA ACTGATGTAT 
AAGATTGCAG ATGAAGAGGC GAAGAAAAGG ACCAAGCCCC TGCGTGTGAA GAAACTCTAC 
GTGCTGTCGG CTCTGCTCAT CGAGCAGTAT CACGAGCAGA TGAAAAACGC CCAGCGCGGC 
AAAGTTAAAG GCAAGAACTC AGAGGCCACT TCTGCTTTGG CTGGCTTGCT AGAAGAGGAG 
GTTCTCTCTA CAACTAGTCG GTTCACAGAT AACGCCTGGA GAGGAGCGGA GGCCTACCAT 
TTCTTTATAC TCGCGCAGCG GCAGCTCTAC GAGGGCTACG TTGACACTGC ATTGAAGACA 
GCTCTTCACC TGAGAGACTA TGAAGACATC ATTCCCTCAG TTGAGATCTA CTCTCTGTTA 
GCACTCTGTG CGTGTGCAAG CAGAGCTTTT GGTACCTGTT CAAAAGCTTT TATTAAACTG 
GAATCTTTAG AGACGCTCAG TGCAGAGCAG AAGCAGCAAT ATGAAGACCT GGCTCTGGAA 
ATCTTCACCA AACACACGCC AAAAGACAAC AGGAAGTCTG AATTAAACAG CCTTCTTGAA 
GGCGGGGAAG GCAAACTGCC GACCTGTATC GCCACTGGCA GCCCAATCAT CGAGTATCAG 
TTCTGGGTGT GCAAAGTCTG CAAGCACTAC GTCCTCGCCC AGGAGATCAG CAACTACAAC 
TTTTGCCCTT TATGCCACAG CTCGGTAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152999.1
CDS	156..3668
Translation	MFFYLSKKIAVPNNVKLKCISWNKDQGFIACGGEDGLLKVLRLETQTDDSKLRGLAAPSNLSMNQNLEGHSGAVQVVTWNEQYQKLTTSDQNGLIIVWMLYKGSWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGKDLKGIQLCHVTWSADSKILLFGMANGEIHIYDNQGNFIMKMKLNCLVNVTGAISIAGIHWYHGTEGYVEPDCPCLAICFDNGRCQIMRHENDQNPVLIDTGMYVVGIQWNHIGSVLAVAGSQKVVTQDKDINIVQFYTPFGEHLGTLKVPGKQMCSLSWEGGGLKIALAVDSFIYFANIRPDYKWGYCSNTVVYAYTRPDRPEYCVVFWDTKNSEKYVKYVKSLISITTCGDFCILATKADENHPQFVLVLCNSIGTPLDPKYIDLVPLFVAMTKTHVIAASKEAFYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDVPSGSVDGVFDYSKAIQGTRDPICAITASDKTLIVGRESGVIQRYSFPNVALIQKYSLDCRACQLSLNCNSSRLAIIDIAGVLTFFDLDTRVTDSTGQQVVGELLKLERKDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKDPEHPSKDYIMNFEIRSLRDSRALIEKVGIEDASQFIEDNPHPRLWRLLAEAALQKLDLYTAQQAFVRCKDYQGIKFVKLLGNLQSESMKQAEVIAYFGRFEDAERMYQDMDRRDLAIGLRMKLGDWFRVLQLLKTGSGDADDSLLEQANNAIGEYFADRQKWQNAVQYYVKGRNQERLAECYYMLEDYEGLETLANSLPENHKLLPEIAQMFVRVGMCEQAVSAFLKCNQPKAAVDTCVHLNQWNKAVELAKSHSMKEIGSLLARYASHLLEKNKTLDAIELYRKASYFFDAAKLMYKIADEEAKKRTKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKNSEATSALAGLLEEEVLSTTSRFTDNAWRGAEAYHFFILAQRQLYEGYVDTALKTALHLRDYEDIIPSVEIYSLLALCACASRAFGTCSKAFIKLESLETLSAEQKQQYEDLALEIFTKHTPKDNRKSELNSLLEGGEGKLPTCIATGSPIIEYQFWVCKVCKHYVLAQEISNYNFCPLCHSSVE

Target ORF information:

RefSeq Version	NM_001159527.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WD repeat domain 35 (Wdr35), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159527.1

ORF Insert Sequence:

ATGTTCTTCT ACCTGAGCAA GAAAATTGCT GTTCCCAATA ATGTGAAGCT GAAATGTATA 
TCCTGGAACA AGGACCAAGG GTTCATAGCA TGTGGCGGTG AGGATGGATT ACTGAAAGTT 
TTAAGACTCG AGACACAGAC AGATGACTCA AAGCTGAGGG GTCTAGCCGC CCCTAGTAAC 
CTTTCCATGA ACCAAAATCT TGAAGGTCAT AGTGGTGCTG TTCAAGTTGT AACGTGGAAT 
GAACAGTATC AGAAGTTGAC TACCAGTGAT CAAAATGGGC TTATCATTGT CTGGATGCTG 
TATAAAGGCT CTTGGTATGA GGAGATGATC AACAATCGCA ACAAGTCTGT GGTTCGAAGC 
ATGAGCTGGA ATGCGGACGG ACAGAAGATC TGCATTGTGT ACGAAGATGG AGCTGTGATC 
GTCGGCTCAG TAGACGGGAA TCGGATTTGG GGGAAAGACC TGAAGGGAAT TCAGCTGTGC 
CATGTGACCT GGTCTGCAGA TAGTAAAATC CTACTTTTTG GAATGGCAAA TGGAGAAATA 
CACATTTATG ATAATCAAGG AAATTTTATA ATGAAAATGA AGCTGAATTG CTTGGTGAAC 
GTCACTGGAG CGATCAGTAT CGCTGGGATT CACTGGTACC ATGGCACCGA AGGCTACGTG 
GAGCCCGATT GCCCTTGCCT GGCAATTTGC TTTGACAATG GGAGATGCCA AATAATGCGG 
CATGAGAATG ACCAAAACCC GGTTTTGATC GACACGGGCA TGTATGTGGT AGGCATCCAG 
TGGAACCACA TCGGCAGTGT GTTAGCTGTG GCCGGCTCCC AGAAGGTAGT CACTCAGGAC 
AAGGACATCA ACATCGTGCA GTTCTACACA CCGTTCGGTG AGCATCTGGG TACTTTGAAA 
GTTCCAGGAA AGCAGATGTG TTCGCTGTCT TGGGAAGGAG GCGGACTGAA GATTGCTCTA 
GCTGTTGACT CCTTTATATA TTTTGCAAAT ATTCGACCTG ATTATAAGTG GGGCTACTGT 
TCCAACACAG TGGTTTATGC GTACACCAGA CCCGATCGTC CCGAATACTG TGTTGTGTTC 
TGGGACACAA AAAACAGTGA GAAATACGTT AAATATGTGA AGAGCCTCAT TTCTATCACC 
ACGTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AGAATCACCC TCAGTTTGTG 
CTTGTCCTTT GCAATTCTAT TGGCACACCC TTGGACCCTA AATACATTGA TCTTGTACCA 
TTATTTGTTG CAATGACCAA AACCCATGTG ATAGCAGCTT CAAAAGAAGC ATTTTATACC 
TGGCAATATC GTGTGGCAAA GAAGCTCACA GCACTGGAAA TTAATCAGAT CACACGGTCT 
CGAAAAGAAG GAAGAGAAAG AATTTATCAC GTTGATGATG TCCCTTCCGG ATCTGTGGAT 
GGGGTGTTTG ATTACAGTAA AGCCATTCAA GGTACAAGGG ATCCAATTTG TGCCATTACT 
GCATCTGATA AGACATTGAT TGTGGGGCGT GAATCTGGCG TCATTCAGAG GTACAGCTTT 
CCTAATGTTG CTCTGATTCA AAAGTATTCC CTGGATTGCC GTGCCTGCCA GTTATCTCTG 
AACTGCAACT CCAGTCGTCT TGCTATCATC GACATCGCTG GAGTCTTGAC TTTCTTTGAC 
TTGGACACTC GGGTGACCGA CAGTACAGGG CAGCAGGTCG TTGGCGAGTT GCTAAAACTG 
GAGCGCAAAG ACGTCTGGGA TATGAAGTGG GCCAAGGACA ATCCTGATTT GTTTGCAATG 
ATGGAGAAGA CGAGAATGTA TGTTTTCAGA AACTTGGATC CTGAGGAACC CATTCAGACC 
TCTGGATATA TTTGCAACTT TGAGGATTTG GAAATTAAGT CTGTTCTCTT GGATGAGATA 
CTGAAGGACC CAGAGCACCC TAGCAAGGAT TACATAATGA ACTTTGAGAT CCGGTCCCTG 
CGAGACAGCC GAGCATTGAT TGAGAAAGTT GGAATTGAAG ATGCGTCTCA GTTCATAGAG 
GACAACCCAC ACCCCCGACT TTGGCGCCTG CTGGCTGAAG CTGCTCTCCA GAAGCTGGAC 
CTGTACACCG CACAGCAAGC GTTTGTGCGC TGCAAGGATT ACCAAGGCAT CAAGTTTGTG 
AAGCTCCTGG GCAATCTGCA GAGCGAGTCA ATGAAGCAGG CTGAAGTTAT TGCCTACTTT 
GGCAGGTTCG AAGATGCCGA AAGGATGTAT CAAGATATGG ACAGACGGGA CCTCGCTATT 
GGTCTCAGAA TGAAATTGGG GGACTGGTTC AGAGTACTGC AGCTCCTGAA AACTGGATCT 
GGTGATGCAG ACGACAGTCT CCTTGAACAA GCTAACAATG CCATTGGAGA GTACTTTGCT 
GACCGACAGA AGTGGCAGAA TGCTGTGCAA TATTATGTAA AAGGCAGGAA CCAGGAGCGC 
CTGGCCGAAT GCTATTATAT GCTAGAAGAT TATGAGGGGT TAGAGACTCT TGCCAATTCA 
CTTCCAGAAA ACCATAAGTT GCTTCCAGAA ATAGCCCAGA TGTTTGTGAG AGTTGGAATG 
TGTGAGCAAG CTGTGAGCGC ATTCTTGAAG TGTAACCAAC CAAAGGCAGC GGTGGACACC 
TGTGTACACC TGAACCAATG GAACAAAGCC GTCGAACTGG CTAAAAGTCA TAGTATGAAG 
GAGATTGGAT CTCTGTTAGC TAGGTACGCG TCCCATTTAC TGGAGAAGAA CAAGACTCTC 
GATGCCATTG AACTCTATAG GAAAGCCAGC TACTTTTTTG ATGCAGCTAA ACTGATGTAT 
AAGATTGCAG ATGAAGAGGC GAAGAAAAGG ACCAAGCCCC TGCGTGTGAA GAAACTCTAC 
GTGCTGTCGG CTCTGCTCAT CGAGCAGTAT CACGAGCAGA TGAAAAACGC CCAGCGCGGC 
AAAGTTAAAG GCAAGAACTC AGAGGCCACT TCTGCTTTGG CTGGCTTGCT AGAAGAGGAG 
GTTCTCTCTA CAACTAGTCG GTTCACAGAT AACGCCTGGA GAGGAGCGGA GGCCTACCAT 
TTCTTTATAC TCGCGCAGCG GCAGCTCTAC GAGGGCTACG TTGACACTGC ATTGAAGACA 
GCTCTTCACC TGAGAGACTA TGAAGACATC ATTCCCTCAG TTGAGATCTA CTCTCTGTTA 
GCACTCTGTG CGTGTGCAAG CAGAGCTTTT GGTACCTGTT CAAAAGCTTT TATTAAACTG 
GAATCTTTAG AGACGCTCAG TGCAGAGCAG AAGCAGCAAT ATGAAGACCT GGCTCTGGAA 
ATCTTCACCA AACACACGCC AAAAGACAAC AGGAAGTCTG AATTAAACAG CCTTCTTGAA 
GGCGGGGAAG GCAAACTGCC GACCTGTATC GCCACTGGCA GCCCAATCAT CGAGTATCAG 
TTCTGGGTGT GCAAAGTCTG CAAGCACTAC GTCCTCGCCC AGGAGATCAG CAACTACAAC 
TTTTGCCCTT TATGCCACAG CTCGGTAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium.
Human molecular genetics24(14)4126-37(2015 Jul)
Caparr?s-Mart?n JA,De Luca A,Cartault F,Aglan M,Temtamy S,Otaify GA,Mehrez M,Valencia M,V?zquez L,Alessandri JL,Nevado J,Rueda-Arenas I,Heath KE,Digilio MC,Dallapiccola B,Goodship JA,Mill P,Lapunzina P,Ruiz-Perez VL

Enhanced expression of WD repeat-containing protein 35 via nuclear factor-kappa B activation in bupivacaine-treated Neuro2a cells.
PloS one9(1)e86336(2014)
Huang L,Kondo F,Harato M,Feng GG,Ishikawa N,Fujiwara Y,Okada S

Enhanced expression of WD repeat-containing protein 35 via CaMKK/AMPK activation in bupivacaine-treated Neuro2a cells.
PloS one9(5)e98185(2014)
Huang L,Kondo F,Gosho M,Feng GG,Harato M,Xia ZY,Ishikawa N,Fujiwara Y,Okada S

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
PLoS genetics8(10)e1002966(2012)
Nakano Y,Jahan I,Bonde G,Sun X,Hildebrand MS,Engelhardt JF,Smith RJ,Cornell RA,Fritzsch B,B?nfi B

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Wdr35 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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