Drc3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Drc3
Entrez Gene ID	74665
Full Name	dynein regulatory complex subunit 3
Synonyms	4930449E07Rik,Lrrc48,m6Bei
General protein information	Preferred Names dynein regulatory complex subunit 3 Names dynein regulatory complex subunit 3 leucine rich repeat containing 48 leucine-rich repeat-containing protein 48 novel Leucine Rich Repeat domain-containing protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006534382.3	XP_006534445.1	dynein regulatory complex subunit 3 isoform X1
XM_006534382.4	XP_006534445.1	dynein regulatory complex subunit 3 isoform X1
NM_029044.2	NP_083320.1	dynein regulatory complex subunit 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LRRC48	XP_003315634.1
Drosophila melanogaster (fruit fly)	Ppr-Y	NP_001015502.2
	LRRC48	XP_001092080.1
Xenopus tropicalis (tropical clawed frog)	lrrc48	NP_001123832.1
Canis lupus familiaris (dog)	LRRC48	XP_536663.3
Mus musculus (house mouse)	Lrrc48	NP_083320.1
Rattus norvegicus (Norway rat)	Lrrc48	NP_001013879.1
Danio rerio (zebrafish)	lrrc48	NP_001071195.1
Gallus gallus (chicken)	LRRC48	XP_414814.1
Drosophila melanogaster (fruit fly)	TbCMF46	NP_609325.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010129	XP_001238137.2
Homo sapiens (human)	LRRC48	NP_112584.3
Bos taurus (cattle)	LRRC48	NP_001095581.1

Gene Ontology
Bibliography

Related articles in PubMed

TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa.
Castaneda JM, Hua R, Miyata H, Oji A, Guo Y, Cheng Y, Zhou T, Guo X, Cui Y, Shen B, Wang Z, Hu Z, Zhou Z, Sha J, Prunskaite-Hyyrylainen R, Yu Z, Ramirez-Solis R, Ikawa M, Matzuk MM, Liu M
Proceedings of the National Academy of Sciences of the United States of America114(27)E5370-E5378(2017 Jul)

Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse.
Stauber M, Weidemann M, Dittrich-Breiholz O, Lobschat K, Alten L, Mai M, Beckers A, Kracht M, Gossler A
Developmental biology423(2)170-188(2017 03)

Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice.
Ha S, Lindsay AM, Timms AE, Beier DR
G3 (Bethesda, Md.)6(8)2479-87(2016 08)

A forward genetic screen in mice identifies mutants with abnormal cortical patterning.
Ha S, Stottmann RW, Furley AJ, Beier DR
Cerebral cortex (New York, N.Y. : 1991)25(1)167-79(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in Lrrc48 cause hydrocephalus, laterality defects, and sinusitis.
Title: Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice.

The following Drc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Drc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15111	NM_029044.2 Latest version!	Mus musculus dynein regulatory complex subunit 3 (Drc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu47041	XM_006534382.4 Latest version!	Mus musculus dynein regulatory complex subunit 3 (Drc3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu47041	XM_006534382.3	Mus musculus leucine rich repeat containing 48 (Lrrc48), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Drc3 ( XM_006534382.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534445.1 Mus musculus leucine rich repeat containing 48 (Lrrc48), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47041
Clone ID Related Accession (Same CDS sequence)	XM_006534382.3 , XM_006534382.4
Accession Version	XM_006534382.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dynein regulatory complex subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATA TCATCTACCT GCGCCGGTTC CCGTGCCTGC GAACACTCAG TCTCGCTGGG 
AATCCCGTTT CTGAGGCAGA GGAGTACAAG ATGTTCATCT ATGCCTATCT TTCTGACCTC 
GTGTACTTGG ACTTCCGGCG CGTTGATGAG CAGATGAGAG AGATGGCGAA GATGAAACAT 
CAGTACAGCA TTGACGAGCT GAAGCACCGC GAGGCACAGC TGCAGATGAA GCTGGAGGAG 
GAGCAGGCCA AGCAGGAGAA GCTGGAGGAA CACAAGATGG CGTTTGTTGA GCACCTGAAC 
GGCCCCTTCC TGTTTGACAG CATGTATTCC GAGGATGTGG AGGGCAACAA GCTGTCCTAC 
CTGCCTGGTG TTAGAGAGCT CCTTGAAGCC TACAAGGACA AGTTTGTCAT CATCTGCCTG 
AACATTTTTG AGTATGGCCT GAATCAGCAA GAAAAACGGA AGGTAGAGTT AGACACTTTC 
AATGAGTGCA TACAGGAGGC CATCCTGGAA AACCAGGACC AGGGCAAGCT CAAGGTTGCC 
AAGTTTGAGG AAAAGCACTT GCTGAACTTA AATGCCATCC GGGAAGAGAC TGATCTGTCC 
AACATCGAGA AGAAGCTGAC GGAGTGCACT GAGAGCATCG GTGAGCTGTT CAACACACTC 
ATGATCCTGG AGATGCAGCT GGTGGAACAG CTGGAGGAGA CCATAAACAT ATTTGAAAGA 
AATATCACTG ACTTGGTGGG ACTATTTATT GAAAATGTCC AAAGTCTGAT TGCTCAGTGC 
CGGGACCTGG AGAACCATCA CCATGAGAAG CTCCTGGAGA TCGCCATCAA CACCCTGGAG 
AAGATCCTCA AGGGCGAGAT GGACGAGGAC TTACCCGATG ACGTGCGGGC GCTTTTTGTT 
GACAAAGACA CAATTGTTAA TGCCGTTGGG GCATCACATG ACATCCACCT CCTGAAGATT 
GACAACCGAG AAGATGAGCT GGTGACTGGA ATCAACTCTT GGTGTGCACA TCTAGTAGAC 
AAGATTCACA AAGATGAGAT CATGAGGAAC CGTAAGCGAG TGAAGGAGAT CAATCAATTC 
GTCGACCACA TGCAGAGTGA ACTGGACAAC CTGGAATGTG GAGACATTAT AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534445.1
CDS	890..2026
Translation	MMNIIYLRRFPCLRTLSLAGNPVSEAEEYKMFIYAYLSDLVYLDFRRVDEQMREMAKMKHQYSIDELKHREAQLQMKLEEEQAKQEKLEEHKMAFVEHLNGPFLFDSMYSEDVEGNKLSYLPGVRELLEAYKDKFVIICLNIFEYGLNQQEKRKVELDTFNECIQEAILENQDQGKLKVAKFEEKHLLNLNAIREETDLSNIEKKLTECTESIGELFNTLMILEMQLVEQLEETINIFERNITDLVGLFIENVQSLIAQCRDLENHHHEKLLEIAINTLEKILKGEMDEDLPDDVRALFVDKDTIVNAVGASHDIHLLKIDNREDELVTGINSWCAHLVDKIHKDEIMRNRKRVKEINQFVDHMQSELDNLECGDIID

Target ORF information:

RefSeq Version	XM_006534382.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat containing 48 (Lrrc48), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534382.3

ORF Insert Sequence:

ATGATGAATA TCATCTACCT GCGCCGGTTC CCGTGCCTGC GAACACTCAG TCTCGCTGGG 
AATCCCGTTT CTGAGGCAGA GGAGTACAAG ATGTTCATCT ATGCCTATCT TTCTGACCTC 
GTGTACTTGG ACTTCCGGCG CGTTGATGAG CAGATGAGAG AGATGGCGAA GATGAAACAT 
CAGTACAGCA TTGACGAGCT GAAGCACCGC GAGGCACAGC TGCAGATGAA GCTGGAGGAG 
GAGCAGGCCA AGCAGGAGAA GCTGGAGGAA CACAAGATGG CGTTTGTTGA GCACCTGAAC 
GGCCCCTTCC TGTTTGACAG CATGTATTCC GAGGATGTGG AGGGCAACAA GCTGTCCTAC 
CTGCCTGGTG TTAGAGAGCT CCTTGAAGCC TACAAGGACA AGTTTGTCAT CATCTGCCTG 
AACATTTTTG AGTATGGCCT GAATCAGCAA GAAAAACGGA AGGTAGAGTT AGACACTTTC 
AATGAGTGCA TACAGGAGGC CATCCTGGAA AACCAGGACC AGGGCAAGCT CAAGGTTGCC 
AAGTTTGAGG AAAAGCACTT GCTGAACTTA AATGCCATCC GGGAAGAGAC TGATCTGTCC 
AACATCGAGA AGAAGCTGAC GGAGTGCACT GAGAGCATCG GTGAGCTGTT CAACACACTC 
ATGATCCTGG AGATGCAGCT GGTGGAACAG CTGGAGGAGA CCATAAACAT ATTTGAAAGA 
AATATCACTG ACTTGGTGGG ACTATTTATT GAAAATGTCC AAAGTCTGAT TGCTCAGTGC 
CGGGACCTGG AGAACCATCA CCATGAGAAG CTCCTGGAGA TCGCCATCAA CACCCTGGAG 
AAGATCCTCA AGGGCGAGAT GGACGAGGAC TTACCCGATG ACGTGCGGGC GCTTTTTGTT 
GACAAAGACA CAATTGTTAA TGCCGTTGGG GCATCACATG ACATCCACCT CCTGAAGATT 
GACAACCGAG AAGATGAGCT GGTGACTGGA ATCAACTCTT GGTGTGCACA TCTAGTAGAC 
AAGATTCACA AAGATGAGAT CATGAGGAAC CGTAAGCGAG TGAAGGAGAT CAATCAATTC 
GTCGACCACA TGCAGAGTGA ACTGGACAAC CTGGAATGTG GAGACATTAT AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Drc3 ( NM_029044.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083320.1 Mus musculus dynein regulatory complex subunit 3 (Drc3), mRNA.

Price & Availability↑

CloneID	OMu15111
Clone ID Related Accession (Same CDS sequence)	NM_029044.2
Accession Version	NM_029044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	dynein regulatory complex subunit 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK015430.1. On Apr 6, 2007 this sequence version replaced NM_029044.1. ##Evidence-Data-START## Transcript exon combination :: AK015430.1, BC018412.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCGGC CATATGACTC GATGGAGCCA AAAGTAATGG ATGATGACAT GCTCAAAGCA 
GCTGTCGGGG AGCAAGGGCC CCAGGAAGAA GCTGGGCAGC TAGCCAAGCA GGAGGGCATC 
CTCTTCAAAG ATGTCCTGTC CCTGCAGCTG GACTTCCAGA ACATCCTCCG CATCGATAAC 
CTCTGGCAGT TTGAGAACCT GAAGAAGCTG CAACTGAACA ACAACATCAT CGAGCGCATC 
GAGGGCCTGA CGAACCTCAT ACACCTCGTC TGGCTGGATT TGTCCTTTAA CAACATCGAG 
GCCATCGAGG GCCTGGATAC CCTGGTCAAC CTGGAGGACC TGAGCTTGTC CAATAACCGG 
ATCTCCAAGG TGGACTCTCT AGATGCCCTG GTGAAGCTGC AGGTGCTGTC ACTGGGCAAC 
AACCAGATTA GCAACATGAT GAATATCATC TACCTGCGCC GGTTCCCGTG CCTGCGAACA 
CTCAGTCTCG CTGGGAATCC CGTTTCTGAG GCAGAGGAGT ACAAGATGTT CATCTATGCC 
TATCTTTCTG ACCTCGTGTA CTTGGACTTC CGGCGCGTTG ATGAGCAGAT GAGAGAGATG 
GCGAAGATGA AACATCAGTA CAGCATTGAC GAGCTGAAGC ACCGCGAGGC ACAGCTGCAG 
ATGAAGCTGG AGGAGGAGCA GGCCAAGCAG GAGAAGCTGG AGGAACACAA GATGGCGTTT 
GTTGAGCACC TGAACGGCCC CTTCCTGTTT GACAGCATGT ATTCCGAGGA TGTGGAGGGC 
AACAAGCTGT CCTACCTGCC TGGTGTTAGA GAGCTCCTTG AAGCCTACAA GGACAAGTTT 
GTCATCATCT GCCTGAACAT TTTTGAGTAT GGCCTGAATC AGCAAGAAAA ACGGAAGGTA 
GAGTTAGACA CTTTCAATGA GTGCATACAG GAGGCCATCC TGGAAAACCA GGACCAGGGC 
AAGCTCAAGG TTGCCAAGTT TGAGGAAAAG CACTTGCTGA ACTTAAATGC CATCCGGGAA 
GAGACTGATC TGTCCAACAT CGAGAAGAAG CTGACGGAGT GCACTGAGAG CATCGGTGAG 
CTGTTCAACA CACTCATGAT CCTGGAGATG CAGCTGGTGG AACAGCTGGA GGAGACCATA 
AACATATTTG AAAGAAATAT CACTGACTTG GTGGGACTAT TTATTGAAAA TGTCCAAAGT 
CTGATTGCTC AGTGCCGGGA CCTGGAGAAC CATCACCATG AGAAGCTCCT GGAGATCGCC 
ATCAACACCC TGGAGAAGAT CCTCAAGGGC GAGATGGACG AGGACTTACC CGATGACGTG 
CGGGCGCTTT TTGTTGACAA AGACACAATT GTTAATGCCG TTGGGGCATC ACATGACATC 
CACCTCCTGA AGATTGACAA CCGAGAAGAT GAGCTGGTGA CTGGAATCAA CTCTTGGTGT 
GCACATCTAG TAGACAAGAT TCACAAAGAT GAGATCATGA GGAACCGTAA GCGAGTGAAG 
GAGATCAATC AATTCGTCGA CCACATGCAG AGTGAACTGG ACAACCTGGA ATGTGGAGAC 
ATTATAGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083320.1
CDS	192..1763
Translation	MSRPYDSMEPKVMDDDMLKAAVGEQGPQEEAGQLAKQEGILFKDVLSLQLDFQNILRIDNLWQFENLKKLQLNNNIIERIEGLTNLIHLVWLDLSFNNIEAIEGLDTLVNLEDLSLSNNRISKVDSLDALVKLQVLSLGNNQISNMMNIIYLRRFPCLRTLSLAGNPVSEAEEYKMFIYAYLSDLVYLDFRRVDEQMREMAKMKHQYSIDELKHREAQLQMKLEEEQAKQEKLEEHKMAFVEHLNGPFLFDSMYSEDVEGNKLSYLPGVRELLEAYKDKFVIICLNIFEYGLNQQEKRKVELDTFNECIQEAILENQDQGKLKVAKFEEKHLLNLNAIREETDLSNIEKKLTECTESIGELFNTLMILEMQLVEQLEETINIFERNITDLVGLFIENVQSLIAQCRDLENHHHEKLLEIAINTLEKILKGEMDEDLPDDVRALFVDKDTIVNAVGASHDIHLLKIDNREDELVTGINSWCAHLVDKIHKDEIMRNRKRVKEINQFVDHMQSELDNLECGDIID

Target ORF information:

RefSeq Version	NM_029044.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein regulatory complex subunit 3 (Drc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029044.2

ORF Insert Sequence:

ATGAGCCGGC CATATGACTC GATGGAGCCA AAAGTAATGG ATGATGACAT GCTCAAAGCA 
GCTGTCGGGG AGCAAGGGCC CCAGGAAGAA GCTGGGCAGC TAGCCAAGCA GGAGGGCATC 
CTCTTCAAAG ATGTCCTGTC CCTGCAGCTG GACTTCCAGA ACATCCTCCG CATCGATAAC 
CTCTGGCAGT TTGAGAACCT GAAGAAGCTG CAACTGAACA ACAACATCAT CGAGCGCATC 
GAGGGCCTGA CGAACCTCAT ACACCTCGTC TGGCTGGATT TGTCCTTTAA CAACATCGAG 
GCCATCGAGG GCCTGGATAC CCTGGTCAAC CTGGAGGACC TGAGCTTGTC CAATAACCGG 
ATCTCCAAGG TGGACTCTCT AGATGCCCTG GTGAAGCTGC AGGTGCTGTC ACTGGGCAAC 
AACCAGATTA GCAACATGAT GAATATCATC TACCTGCGCC GGTTCCCGTG CCTGCGAACA 
CTCAGTCTCG CTGGGAATCC CGTTTCTGAG GCAGAGGAGT ACAAGATGTT CATCTATGCC 
TATCTTTCTG ACCTCGTGTA CTTGGACTTC CGGCGCGTTG ATGAGCAGAT GAGAGAGATG 
GCGAAGATGA AACATCAGTA CAGCATTGAC GAGCTGAAGC ACCGCGAGGC ACAGCTGCAG 
ATGAAGCTGG AGGAGGAGCA GGCCAAGCAG GAGAAGCTGG AGGAACACAA GATGGCGTTT 
GTTGAGCACC TGAACGGCCC CTTCCTGTTT GACAGCATGT ATTCCGAGGA TGTGGAGGGC 
AACAAGCTGT CCTACCTGCC TGGTGTTAGA GAGCTCCTTG AAGCCTACAA GGACAAGTTT 
GTCATCATCT GCCTGAACAT TTTTGAGTAT GGCCTGAATC AGCAAGAAAA ACGGAAGGTA 
GAGTTAGACA CTTTCAATGA GTGCATACAG GAGGCCATCC TGGAAAACCA GGACCAGGGC 
AAGCTCAAGG TTGCCAAGTT TGAGGAAAAG CACTTGCTGA ACTTAAATGC CATCCGGGAA 
GAGACTGATC TGTCCAACAT CGAGAAGAAG CTGACGGAGT GCACTGAGAG CATCGGTGAG 
CTGTTCAACA CACTCATGAT CCTGGAGATG CAGCTGGTGG AACAGCTGGA GGAGACCATA 
AACATATTTG AAAGAAATAT CACTGACTTG GTGGGACTAT TTATTGAAAA TGTCCAAAGT 
CTGATTGCTC AGTGCCGGGA CCTGGAGAAC CATCACCATG AGAAGCTCCT GGAGATCGCC 
ATCAACACCC TGGAGAAGAT CCTCAAGGGC GAGATGGACG AGGACTTACC CGATGACGTG 
CGGGCGCTTT TTGTTGACAA AGACACAATT GTTAATGCCG TTGGGGCATC ACATGACATC 
CACCTCCTGA AGATTGACAA CCGAGAAGAT GAGCTGGTGA CTGGAATCAA CTCTTGGTGT 
GCACATCTAG TAGACAAGAT TCACAAAGAT GAGATCATGA GGAACCGTAA GCGAGTGAAG 
GAGATCAATC AATTCGTCGA CCACATGCAG AGTGAACTGG ACAACCTGGA ATGTGGAGAC 
ATTATAGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Drc3 ( XM_006534382.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534445.1 Mus musculus dynein regulatory complex subunit 3 (Drc3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47041
Clone ID Related Accession (Same CDS sequence)	XM_006534382.3 , XM_006534382.4
Accession Version	XM_006534382.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dynein regulatory complex subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534382.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATA TCATCTACCT GCGCCGGTTC CCGTGCCTGC GAACACTCAG TCTCGCTGGG 
AATCCCGTTT CTGAGGCAGA GGAGTACAAG ATGTTCATCT ATGCCTATCT TTCTGACCTC 
GTGTACTTGG ACTTCCGGCG CGTTGATGAG CAGATGAGAG AGATGGCGAA GATGAAACAT 
CAGTACAGCA TTGACGAGCT GAAGCACCGC GAGGCACAGC TGCAGATGAA GCTGGAGGAG 
GAGCAGGCCA AGCAGGAGAA GCTGGAGGAA CACAAGATGG CGTTTGTTGA GCACCTGAAC 
GGCCCCTTCC TGTTTGACAG CATGTATTCC GAGGATGTGG AGGGCAACAA GCTGTCCTAC 
CTGCCTGGTG TTAGAGAGCT CCTTGAAGCC TACAAGGACA AGTTTGTCAT CATCTGCCTG 
AACATTTTTG AGTATGGCCT GAATCAGCAA GAAAAACGGA AGGTAGAGTT AGACACTTTC 
AATGAGTGCA TACAGGAGGC CATCCTGGAA AACCAGGACC AGGGCAAGCT CAAGGTTGCC 
AAGTTTGAGG AAAAGCACTT GCTGAACTTA AATGCCATCC GGGAAGAGAC TGATCTGTCC 
AACATCGAGA AGAAGCTGAC GGAGTGCACT GAGAGCATCG GTGAGCTGTT CAACACACTC 
ATGATCCTGG AGATGCAGCT GGTGGAACAG CTGGAGGAGA CCATAAACAT ATTTGAAAGA 
AATATCACTG ACTTGGTGGG ACTATTTATT GAAAATGTCC AAAGTCTGAT TGCTCAGTGC 
CGGGACCTGG AGAACCATCA CCATGAGAAG CTCCTGGAGA TCGCCATCAA CACCCTGGAG 
AAGATCCTCA AGGGCGAGAT GGACGAGGAC TTACCCGATG ACGTGCGGGC GCTTTTTGTT 
GACAAAGACA CAATTGTTAA TGCCGTTGGG GCATCACATG ACATCCACCT CCTGAAGATT 
GACAACCGAG AAGATGAGCT GGTGACTGGA ATCAACTCTT GGTGTGCACA TCTAGTAGAC 
AAGATTCACA AAGATGAGAT CATGAGGAAC CGTAAGCGAG TGAAGGAGAT CAATCAATTC 
GTCGACCACA TGCAGAGTGA ACTGGACAAC CTGGAATGTG GAGACATTAT AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534445.1
CDS	927..2063
Translation	MMNIIYLRRFPCLRTLSLAGNPVSEAEEYKMFIYAYLSDLVYLDFRRVDEQMREMAKMKHQYSIDELKHREAQLQMKLEEEQAKQEKLEEHKMAFVEHLNGPFLFDSMYSEDVEGNKLSYLPGVRELLEAYKDKFVIICLNIFEYGLNQQEKRKVELDTFNECIQEAILENQDQGKLKVAKFEEKHLLNLNAIREETDLSNIEKKLTECTESIGELFNTLMILEMQLVEQLEETINIFERNITDLVGLFIENVQSLIAQCRDLENHHHEKLLEIAINTLEKILKGEMDEDLPDDVRALFVDKDTIVNAVGASHDIHLLKIDNREDELVTGINSWCAHLVDKIHKDEIMRNRKRVKEINQFVDHMQSELDNLECGDIID

Target ORF information:

RefSeq Version	XM_006534382.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein regulatory complex subunit 3 (Drc3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534382.4

ORF Insert Sequence:

ATGATGAATA TCATCTACCT GCGCCGGTTC CCGTGCCTGC GAACACTCAG TCTCGCTGGG 
AATCCCGTTT CTGAGGCAGA GGAGTACAAG ATGTTCATCT ATGCCTATCT TTCTGACCTC 
GTGTACTTGG ACTTCCGGCG CGTTGATGAG CAGATGAGAG AGATGGCGAA GATGAAACAT 
CAGTACAGCA TTGACGAGCT GAAGCACCGC GAGGCACAGC TGCAGATGAA GCTGGAGGAG 
GAGCAGGCCA AGCAGGAGAA GCTGGAGGAA CACAAGATGG CGTTTGTTGA GCACCTGAAC 
GGCCCCTTCC TGTTTGACAG CATGTATTCC GAGGATGTGG AGGGCAACAA GCTGTCCTAC 
CTGCCTGGTG TTAGAGAGCT CCTTGAAGCC TACAAGGACA AGTTTGTCAT CATCTGCCTG 
AACATTTTTG AGTATGGCCT GAATCAGCAA GAAAAACGGA AGGTAGAGTT AGACACTTTC 
AATGAGTGCA TACAGGAGGC CATCCTGGAA AACCAGGACC AGGGCAAGCT CAAGGTTGCC 
AAGTTTGAGG AAAAGCACTT GCTGAACTTA AATGCCATCC GGGAAGAGAC TGATCTGTCC 
AACATCGAGA AGAAGCTGAC GGAGTGCACT GAGAGCATCG GTGAGCTGTT CAACACACTC 
ATGATCCTGG AGATGCAGCT GGTGGAACAG CTGGAGGAGA CCATAAACAT ATTTGAAAGA 
AATATCACTG ACTTGGTGGG ACTATTTATT GAAAATGTCC AAAGTCTGAT TGCTCAGTGC 
CGGGACCTGG AGAACCATCA CCATGAGAAG CTCCTGGAGA TCGCCATCAA CACCCTGGAG 
AAGATCCTCA AGGGCGAGAT GGACGAGGAC TTACCCGATG ACGTGCGGGC GCTTTTTGTT 
GACAAAGACA CAATTGTTAA TGCCGTTGGG GCATCACATG ACATCCACCT CCTGAAGATT 
GACAACCGAG AAGATGAGCT GGTGACTGGA ATCAACTCTT GGTGTGCACA TCTAGTAGAC 
AAGATTCACA AAGATGAGAT CATGAGGAAC CGTAAGCGAG TGAAGGAGAT CAATCAATTC 
GTCGACCACA TGCAGAGTGA ACTGGACAAC CTGGAATGTG GAGACATTAT AGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa.
Proceedings of the National Academy of Sciences of the United States of America114(27)E5370-E5378(2017 Jul)
Castaneda JM,Hua R,Miyata H,Oji A,Guo Y,Cheng Y,Zhou T,Guo X,Cui Y,Shen B,Wang Z,Hu Z,Zhou Z,Sha J,Prunskaite-Hyyrylainen R,Yu Z,Ramirez-Solis R,Ikawa M,Matzuk MM,Liu M

Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse.
Developmental biology423(2)170-188(2017 03)
Stauber M,Weidemann M,Dittrich-Breiholz O,Lobschat K,Alten L,Mai M,Beckers A,Kracht M,Gossler A

Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice.
G3 (Bethesda, Md.)6(8)2479-87(2016 08)
Ha S,Lindsay AM,Timms AE,Beier DR

A forward genetic screen in mice identifies mutants with abnormal cortical patterning.
Cerebral cortex (New York, N.Y. : 1991)25(1)167-79(2015 Jan)
Ha S,Stottmann RW,Furley AJ,Beier DR

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Drc3 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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