Eaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eaf1
Entrez Gene ID	74427
Full Name	ELL associated factor 1
Synonyms	4933403C17Rik,AI666536
General protein information	Preferred Names ELL associated factor 1 Names ELL-associated factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 B

mRNA and Protein(s)

mRNA	Protein	Name
NM_028932.4	NP_083208.1	ELL-associated factor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EAF1	NP_149074.3
Rattus norvegicus (Norway rat)	Eaf1	NP_001100763.1
Pan troglodytes (chimpanzee)	EAF1	XP_001159232.1
	EAF1	XP_001082128.2
Mus musculus (house mouse)	Eaf1	NP_083208.1
Danio rerio (zebrafish)	eaf1	NP_001002115.1
Xenopus tropicalis (tropical clawed frog)	eaf1	NP_001072894.1
Gallus gallus (chicken)	EAF1	XP_001235897.3
Canis lupus familiaris (dog)	EAF1	XP_542776.4
Bos taurus (cattle)	EAF1	NP_001179082.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

?H NMR metabolic profiling of plasma reveals additional phenotypes in knockout mouse models.
Probert F, Rice P, Scudamore CL, Wells S, Williams R, Hough TA, Cox IJ
Journal of proteome research14(5)2036-45(2015 May)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line.
Shu H, Chen S, Bi Q, Mumby M, Brekken DL
Molecular & cellular proteomics : MCP3(3)279-86(2004 Mar)

Identification of a novel tissue-specific transcriptional activator FESTA as a protein that interacts with the transcription elongation factor S-II.
Saso K, Ito T, Natori S, Sekimizu K
Journal of biochemistry133(4)493-500(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06330	NM_028932.4 Latest version!	Mus musculus ELL associated factor 1 (Eaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eaf1 ( NM_028932.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083208.1 Mus musculus ELL associated factor 1 (Eaf1), mRNA.

Price & Availability↑

CloneID	OMu06330
Clone ID Related Accession (Same CDS sequence)	NM_028932.4
Accession Version	NM_028932.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	ELL-associated factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK044181.1, AK036661.1 and AK044737.1. On May 16, 2009 this sequence version replaced NM_028932.3. ##Evidence-Data-START## Transcript exon combination :: AK044737.1, AK044181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAACGGCA CGGCGAACCC GCTGCTGGAC CGGGAGGAAC ACTGTCTGCG GCTAGGGGAA 
AGCTTCGAGA AGCGGCCGCG GGCCTCCTTC CACACGATTC GCTACGATTT TAAGCCAGCA 
TCAATAGACA CTTCCTGTGA AGGAGAACTC CAGGTTGGCA AAGGAGATGA AGTCACCATT 
ACGCTGCCAC ACATCCCTGG GTCCACGCCG CCAATGACGG TGTTCAAAGG GAACAAGCGG 
CCCTATCAGA AAGACTGTGT GCTCATTATT AACCACGACA CTGGAGAGTA TGTGCTGGAG 
AAGCTGAGCA GCAGCATTCA GGTCAAGAAG ACAAGAGCTG AGGGAAGCAG TAAAATCCAA 
GCTCGAATGG AGCAGCAGCC CGCTCGTCCC CCACAGCCGT CACAGCCACC CCCTCCACCT 
CCACCCATGC CGTTCCGAGC CCCAACGAAG CCTCCAGCTG GACCCAAAAC TTCTCCCCTG 
AAGGATAACC CCTCACCTGA GCCCCAGTTG GATGACATCA AAAGAGAGCT GAGGGCCGAG 
GTGGACATTA TTGAGCAGAT GAGCAGCAGC AGTGGGAGCA GCTCCTCCGA CTCTGAGAGC 
TCCTCGGGAA GTGACGACGA CAGCTCCAGC AGCGCAGGCG AAGACAATGG CCCAGCCTCG 
CCTCCCCAGC CCTCACACCA GCAGCCCTAC AACAGCAGGC CTGCGGTGGC CAATGGAACC 
AGCCGGCCCC AAGGAAGCTC TCAGCTCATG AACACGCTCA GAAATGACCT GCAGCTAAGT 
GAGTCTGGCA GTGACAGTGA TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083208.1
CDS	191..997
Translation	MNGTANPLLDREEHCLRLGESFEKRPRASFHTIRYDFKPASIDTSCEGELQVGKGDEVTITLPHIPGSTPPMTVFKGNKRPYQKDCVLIINHDTGEYVLEKLSSSIQVKKTRAEGSSKIQARMEQQPARPPQPSQPPPPPPPMPFRAPTKPPAGPKTSPLKDNPSPEPQLDDIKRELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSSSAGEDNGPASPPQPSHQQPYNSRPAVANGTSRPQGSSQLMNTLRNDLQLSESGSDSDD

Target ORF information:

RefSeq Version	NM_028932.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ELL associated factor 1 (Eaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028932.4

ORF Insert Sequence:

ATGAACGGCA CGGCGAACCC GCTGCTGGAC CGGGAGGAAC ACTGTCTGCG GCTAGGGGAA 
AGCTTCGAGA AGCGGCCGCG GGCCTCCTTC CACACGATTC GCTACGATTT TAAGCCAGCA 
TCAATAGACA CTTCCTGTGA AGGAGAACTC CAGGTTGGCA AAGGAGATGA AGTCACCATT 
ACGCTGCCAC ACATCCCTGG GTCCACGCCG CCAATGACGG TGTTCAAAGG GAACAAGCGG 
CCCTATCAGA AAGACTGTGT GCTCATTATT AACCACGACA CTGGAGAGTA TGTGCTGGAG 
AAGCTGAGCA GCAGCATTCA GGTCAAGAAG ACAAGAGCTG AGGGAAGCAG TAAAATCCAA 
GCTCGAATGG AGCAGCAGCC CGCTCGTCCC CCACAGCCGT CACAGCCACC CCCTCCACCT 
CCACCCATGC CGTTCCGAGC CCCAACGAAG CCTCCAGCTG GACCCAAAAC TTCTCCCCTG 
AAGGATAACC CCTCACCTGA GCCCCAGTTG GATGACATCA AAAGAGAGCT GAGGGCCGAG 
GTGGACATTA TTGAGCAGAT GAGCAGCAGC AGTGGGAGCA GCTCCTCCGA CTCTGAGAGC 
TCCTCGGGAA GTGACGACGA CAGCTCCAGC AGCGCAGGCG AAGACAATGG CCCAGCCTCG 
CCTCCCCAGC CCTCACACCA GCAGCCCTAC AACAGCAGGC CTGCGGTGGC CAATGGAACC 
AGCCGGCCCC AAGGAAGCTC TCAGCTCATG AACACGCTCA GAAATGACCT GCAGCTAAGT 
GAGTCTGGCA GTGACAGTGA TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

?H NMR metabolic profiling of plasma reveals additional phenotypes in knockout mouse models.
Journal of proteome research14(5)2036-45(2015 May)
Probert F,Rice P,Scudamore CL,Wells S,Williams R,Hough TA,Cox IJ

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line.
Molecular & cellular proteomics : MCP3(3)279-86(2004 Mar)
Shu H,Chen S,Bi Q,Mumby M,Brekken DL

Identification of a novel tissue-specific transcriptional activator FESTA as a protein that interacts with the transcription elongation factor S-II.
Journal of biochemistry133(4)493-500(2003 Apr)
Saso K,Ito T,Natori S,Sekimizu K

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Eaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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