Spag17 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spag17
Entrez Gene ID	74362
Full Name	sperm associated antigen 17
Synonyms	4931427F14Rik,PF6,Spag17-ps
General protein information	Preferred Names sperm associated antigen 17 Names sperm-associated antigen 17 projection protein PF6 homolog sperm associated antigen 17, pseudogene
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_028892.4	NP_083168.3	sperm-associated antigen 17

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100170502	NP_001123754.1
Homo sapiens (human)	SPAG17	NP_996879.1
Pan troglodytes (chimpanzee)	SPAG17	XP_513687.2
	SPAG17	XP_002801770.1
Canis lupus familiaris (dog)	SPAG17	XP_849615.1
Mus musculus (house mouse)	Spag17	NP_083168.3
Gallus gallus (chicken)	SPAG17	XP_416538.4
Bos taurus (cattle)	SPAG17	NP_001193803.1
Rattus norvegicus (Norway rat)	Spag17	XP_006233092.1
Danio rerio (zebrafish)	si:dkey-208b23.5	XP_689610.5

Gene Ontology
Bibliography

Related articles in PubMed

Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse.
Stauber M, Weidemann M, Dittrich-Breiholz O, Lobschat K, Alten L, Mai M, Beckers A, Kracht M, Gossler A
Developmental biology423(2)170-188(2017 03)

Spag17 deficiency results in skeletal malformations and bone abnormalities.
Teves ME, Sundaresan G, Cohen DJ, Hyzy SL, Kajan I, Maczis M, Zhang Z, Costanzo RM, Zweit J, Schwartz Z, Boyan BD, Strauss JF 3rd
PloS one10(5)e0125936(2015)

Sperm-associated antigen-17 gene is essential for motile cilia function and neonatal survival.
Teves ME, Zhang Z, Costanzo RM, Henderson SC, Corwin FD, Zweit J, Sundaresan G, Subler M, Salloum FN, Rubin BK, Strauss JF 3rd
American journal of respiratory cell and molecular biology48(6)765-72(2013 Jun)

Dissecting the axoneme interactome: the mammalian orthologue of Chlamydomonas PF6 interacts with sperm-associated antigen 6, the mammalian orthologue of Chlamydomonas PF16.
Zhang Z, Jones BH, Tang W, Moss SB, Wei Z, Ho C, Pollack M, Horowitz E, Bennett J, Baker ME, Strauss JF 3rd
Molecular & cellular proteomics : MCP4(7)914-23(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spag17 plays a role in skeletal growth and mineralization.Spag17 deficiency results in skeletal malformations and bone abnormalities.
Title: Spag17 deficiency results in skeletal malformations and bone abnormalities.

Deletion of Spag17 gene expression results in hydrocephalus and pulmonary and nasal mucus and fluid congestion.
Title: Sperm-associated antigen-17 gene is essential for motile cilia function and neonatal survival.

The following Spag17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spag17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21196	NM_028892.4 Latest version!	Mus musculus sperm associated antigen 17 (Spag17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spag17 ( NM_028892.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083168.3 Mus musculus sperm associated antigen 17 (Spag17), mRNA.

Price & Availability↑

CloneID	OMu21196
Clone ID Related Accession (Same CDS sequence)	NM_028892.4
Accession Version	NM_028892.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 17
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029691.1, AY792594.1, AL606749.13, AK029947.1, BX639948.1, AC102209.11 and AK136364.1. On Dec 11, 2007 this sequence version replaced NM_028892.3. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGCACCCA AGAAGGAGAA ACCTACCGGG AGCGCAAACT ATAAGATATG GGAACCTTCC 
CTCATAGCTG CACACTTGAA CCAGAATGAT TGGAAGGCCT CCATCGCCTT CGTGGTTGGG 
AACCGAGTTG AGGATGATCT TCTCATTCAT GCCCTTGACC TGGCTGTTCG ACTCCCTCAG 
CGGAAACTCT TCAGTATTGT GTCATGGGAA GACATTCTGC AGCAGATGGA TGAAATACAG 
TCACTTGCTG AAAGTGCTTC AGCTAAAAAG GGGAAAAAGC CCACATCTGT CAATTTACCG 
TTACATTACG AGGTGTTCCT GGCAGCAAAG ATAATCATGG AGAGTGGAGA GAAACTAACC 
TTACCATTGA TAGGGAAACT CTTGAAGTGT CAACTTCTCC ATATTAAATC CAAGGACCAG 
CAGAGACGAG AAAATGAAAA GAAGATGGTA GAAGAAAGAA CAAAATCAGA AAAAGACAAA 
GGAAAAGGGA AGTCTCCAAA GGAGAAGAAA GTTGCCAGTG CCAAGCCTGG GAAGGGAAAG 
AGCAAGGACC AGCCTGAGGC CACTGTAACA GTGAGGAAGA CCACACAGTT AAAACGGAGG 
GGAGAAGATG ACGAGGCCAA ATCCTATATC GATGATGAGC CAGACGATGG TGCCCAATAT 
TACATTATTG TTGTGGGCTT CAACAATCCT CAGCTATTGG CCATTATGAC TGAGCTTGGC 
ATTCCTATAA CCAGTGTGAT TAAAATATCT TCAGAGAACT ATGAACCTCT ACAGACACAC 
CTGGCAGCAG TCAGGCAGCA ACAGGAAGCT GTCCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA GAAAAAATTC CATCAAGGAA CTTGAGGTTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACT TGAAATACAT CTCTTTGATG TTGCTCGTCT CCAGTACATG 
GTCAAGGCAA CATATTTTCC TTCTGATTGG TCAGACAATG AACAGATGCT TGCATTGGGC 
ACCGAGATCT TTGAAAACAT TGCCTGTTTG ATGTACGATA GCCTGGACTG GAAGAGGCAA 
CATCATCACT ATTTGCAAAG CATGCAGCTC ATCAATGTCC CACAGGTGGT CAGTGAGAAG 
ACCGTGCTAG AGGCAATCAC AATCCCAGAG CCTCCACCAT CAACTGCTCC AGCCCCCACT 
GGGAAGAAGA AAGCACAGTA TGAGGAGTCG CATGCTCCAC CAACAGTGGC TTTTATCATC 
ACAACTGAAG TAGACATGCG GTATTACAAT GACCTGCTGA ATCCAATTCC AGAGGAATTT 
ATTTCTGTGT CCTTGATACT GCACTGCATG GTGGAACAGG TTGTAGCAAC TGAAGAAGAT 
CTCATCCCGC CAAGTTTGGT GGAGCCAGCC CCCAGAGCAG ACGGGTTAGA CTACAGAATC 
GCAGCTCACA TTGTGTCGAT CCTGCCTTCA CTCTGCCTCT CCGAAAAGGA AAAGAAGAAT 
CTTCGTGAGA TATTCTTAAC TGAAGGAGAG AGTGAAAGCA AAGCCCTGCC CAAAGGTCCC 
CTCCTACTGA ACTACCACGA TGCGCATGCC CACAAGAAGT ATGCACTGAA GGACCAAAAG 
AATTTTGACC CGGTTCAAGT TGAGCAGGAG ATGCAGTCCA AGCTGCCACT GTGGGAATTC 
CTTCAGTTCC CTCTCCCCCC TCCATGGAAC AGCACTAAAC GACTCGCCAC TATTCACGAG 
CTTATGCATT TCTGTACGAA CGAAGTTCTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTATTTACTT TTGAGAGCCT GAAGCTCTCA GAGGTGGATG AGGAAGGGAG GCTCAAGCCC 
ACAGAAACGA CCAGTGACAC AGATGTTGAA AATTTCAACA TTCCTTGGGA CAATCCTGCC 
AGGTTCGCTA AGCTGATAAG GCAGCGGTAC ATTCACAGGA TGAGCATGCA GAAGGCCCCG 
CCAGTAGTAG TAGAGATTGA AAACACTGAG AGAACACTAT TTGTGAACAA GAATTTTGCC 
AAAGCTGAGC AAGATGCACA AGGAGATGAA AATTCCCCAA ATTCTGACGA GCCTGATGCT 
ATTAGTGTGA CGGGTTCTAC TTCCAACTCC ACAAAACCCT GGAATTCTAG TAATAGACAG 
TTCTCAGAAA AAGAGACCAG TGGGTCCATG TGGCCCCAGC CAGAGTCCAT GGATCAGACC 
ATGGACACTG AGATCAAAGA TGACGCAGCC ACAAAGGATG ATTCTCCTGA AAAAAAACCC 
AAGAAGATGG TGGTGGAAGC CGACATAGAG GACATTAAAA AAACGCAACA GCGCAGTTTA 
ATGGACTGGA GCTTTACTGA ATATTTTCAG CCAAAAGTCC TGCTTCAGGT TCTTCAGGAG 
GCTCACCAGC AATACCGATG TGTTGACTCT TACTACCACA CGCAGGATAA CTCTTTACTT 
CTGGTTTTTC ATAATCCAAT GAATCTACAG CGTCTGCAAT GTGAACATTG GAATATTGCT 
CTTCACTCCA ACGTCGGATT CAGGAATTAC TTAGAACTTG TTGCAAAATC TATTGAAGAC 
TGGGTCACAC AGGAAGAAGC GAAGTACCAG GAAGCCAAAA TGGCTGAGGA GCTCAACAGG 
ATCAGGATTG AGTTGGAACT GAAAGCCACT GTCAAAACTT CTGCTAGCAA AATTCCTGGC 
CCCAAAAGAT CTAAAACTAA CAAAGTATCC AGCAAAACAG AGCTATCAGA CCAAGAAAAA 
GATAAAGAAA AGGAAAAAGA CAAGATCCCC TTCGTATTAG AAGGCTCTCT CAAGGCATGG 
AAGGAAGAAC AAGAGAGGTT GGCTGAGGAG GAACGCCTGA AGGAGGAAAA GAAAGCAGAG 
AAGAAGGGAA AGGATACAGG GAAAAAGAAA GGCAAGGATA AGGCCGACAA AGACGATGCT 
AAGGCTTTGA AGAAAAAGTC TTCTTCCAAG GAGAAGCCCA AAGAAGAGCC AGCAAAGACG 
CTGGAAGTGA TAGAGGAAAC CGCCCCACTT CCGGTACCGG AAGTCGTTTA TCCGTTCCGA 
GGATACAATA TGGGAGACAT ACCTATCCAA ATCTCAGGAA CAAATTACTA TCTGTATCCT 
TCTGATGGTG GGCAGATTGA AGTGGAAAAG ACCAGGTTTG AACGAGGGTC AACTTTCATC 
AAAGTCAAAG TAAAAAAAGA TAAACACAAT TTTATAATTC ACCTGAAAGA CCCAAAGGAA 
ATTGTGAAAA AGGAAAAGGA AGAGAAGAAC TCAGAAGAGG AGGAGGAGGA GGAAGAGGAG 
AAGGAGGAGG TAGAAGAGAA GAAGCCGAAG GAGGGGGAAG AGGAAAAGGT TAAACAGAAA 
GTGGAAATGA AAAGAGCACA GATAGAGAAG GAAGCTGTCA GCAAGTTTGG ATCTTTTTCT 
GCCACCTTGG AAAATGGAAT CTGCCTCTCG ATAAGTTACT ATGGATCAAA TGGAATGGCA 
CCAGAGGTTA TAAATTCTGA GTTGGAAGCA ATGATGAATA TTCCTTCAGC TATGACAGCA 
ACAGTGGTTC CTGCTGTAGT GACTGTTCCC CAAGGCAAAG GGAAAGCTAA GCCGAAAGGC 
AAAGAAAAAC ACAAAGATTC CATTAAAGAA GAGGAACTTC CAAAAGAAGA AGAGAAGAAG 
AATCACATAC AGGAAGAAGT AGAACCAGAG ATCGTTATAC AGGAATCACC ACCCTATGTA 
CCCACCTTCC AAAACTTAAA TGTATCTTGC CCCAGTGGGC TCCTGCTGAC CTTCATTGGG 
CAAGAATCTA CAGATTATTC CATCGTAGAT GAAGAACCCA CCCGGAACCT CATGATCCGC 
CAGAGCTACC CCCAGCGGTT GAAGCACTAT GAGTTCTATA AAGCAGTGAT GCCACCCCTG 
GAGCAGGAGG CATCAAGAGT TGTCACCAGC CAGGGCACCG TTATCAAGTA TATGCTGGAT 
GGATCCACAC AGATTCTCTT TGCAGATGGT GCTGTGAGCA GCAGCCCTGA TTCGGGTCCT 
GTTTACACAT CTCCTGAATT GCCTACAAGC CCCCATAATG GAGATTTGGT GGACAGTGCC 
TCTCAGCCCA AATCAGAAAC AGGACCTGAG ATCATCATTA CTAAGAAAGG GAAAGGTCAC 
AAAAATCAAA CAGTGGCAAA TAAGAGTGAA ACCCATGATA TTATCCCAGA GGTACCTCCA 
CCAACTCCCG TAGAGTCCCA CATTGGCACC TGGTTTACCA CAACACCTGA TGGAAGGAGG 
ATTGGCACCA AAGGACTAGA GAAAATTGAG GACTTGAAAC CATATTTGTT CTTCCAGGCT 
ACAGATCCCA TCAATGGAAC GGTCATGACT ACTCGAGAAG ACAAGGTGAT CATTGTTGAG 
AAGAAAGACG GGACTCGGGT TGTGGACCAT GCTGATGGTA CCAGAATCAC AACCTTTTAC 
CAAGTGTATG AAGATCATAT TACTCCCTCA AATGATGAAG AAACAACTGA AGGTCCAAGG 
ACTGTCACTA GACAGGTGAA GTGTATGCGG ATAGAAAGCT CACACTATGC CACTATTATC 
ACCAACTGCG AGGACAGTAG CTGCTGTGCC ACCTTTGGGG ATGGAACATC TATTATTGCA 
AAGCCACAGG GGTCATACCA GGTGCTACCT CCAAACACAG GCTGCCTTTA TATTGATAAA 
GACTGCTCAG CTACATACTG CCATGAATCA AGCAATAATT TGTACCATCC CTTCCAGAAG 
CGTGAGCAGC TGAGAGCCAG CAGGTATATC ATGAAGCACA CCTCAGAGGT GATCTGTGAG 
GTCCAGGATC CTGAGGGGAA CACCTTTCAG GTCATGGCTG ATGGCTCTGT ATCAACAACA 
TTACCTAAAA AGAAACTAGA AGATGACTTC AATGTACAGA TGGAGGGCTA CGAAAGCTTA 
TCGTCACTTC ACCTTGAAAA GAATCACATG CAGATCTATG GTGAACATGT TCCCAGATTT 
TTTGTTGTCT ATACTGATGG ATCGGGAGTG GAACTTCTCC GAGACAGTGA CATAGAAGAA 
TACTTATCCT TGGCGTATGG AGAGTCAACG ACTGTGGTTC TCCAGGAACC AGTACAGGAA 
TACCCAGGTG CACTGAGCAT CACAGTCCTT CGCCCTTTCC ATGAAGCATC ACAATGGATC 
ATGAAGAAGG AATTAGACAC CATAGTTCCT CCAAATCTCC AGTCAAGGTC ATGGGAAAGG 
TTTCCTTCAG TTGAGAAAAA GACTCCAGGA CCACCATTTG GCACTCAAAT TTGGAAAGGC 
CTCTCTATTG GGTCTAAGCA GCTGACAAAC ATCCCAGCCC CTATACTCGA AGGACCCAAA 
GTGCTACAGA TGCGTCAGTT CATTCAGCAT GAAGTCATAA AGAATGAGGT GAAACTAAAG 
CTACAGATTT CCCTTAAGGA CTATATAAAC CACATCCTGA AGAAAGAAGA TGAGCTACAG 
GAGATGACAG TCAAGGATTC CAGAACCGAG GAGGAGAGGG GCAATGCTGC AGATCTCCTC 
AAGCTGGTTA TGTCTTTCCC GAAAATGGAG GAAACTACAA AAAGTCATAT GACTAAAGTT 
GCAGCCCACC TAACAGTTTT ATTCAAGCAA TCTATGGCTT CGGCTCCAAA ATGTTCACCA 
GACAGCTACT CTAAAGAATT CCTTGAAAAG AAGTGGAGGA GTCTGTCACA GGGAACGAGC 
TGGAAAGAAA AGCTTGAGCA GCAGAGGAAC AATATCAAGA AAACACAAAG CTACCTAATG 
CAAATTAAGA CAAAAGAAGT AACACCTTAT TTTAAGTCCG AATTGAGCTC GTTATTTAAG 
TCAAAGTATG ATTATCTGGA GAAGTTTTCC AAAAGCCTGC CTCCTTTTGT GAAGAAAAAT 
GAGGCCAAAC TCATGACAGC TGTTCCGGAT CTCTACTCGG ATTCTACACT GACCGTAGAT 
ACAGAAAAAG AAGCCTCAAA TACACATCCC CTTCTAAACC AGGAGGTTGC TGAGAATATC 
CAAGAGTCTC CTAGGGAAAA AGAAACGGAA TACGTAAACA AATCTCTCCA GACCTCTTCT 
TCCCAAAACC AATATGAAAA TGTAACAATA TCTCCCAAGG AGTCTGTTTA TCAAAGTCAA 
ACTGAAATTG AAACCAAAGA TACCAAGGAA TCTGCTATCC AAAACTTCAC TGAAAAGTTT 
AAGAAATATA CCAAGAAGTC TGCTAGCCAA AACGAAATTG AAGATCTAAT AAAATCTACC 
AAGGAGTCTG TTAGCCAAAG ACAAACTGAA AATGTCACCA GACCTCCTAC TGAAGAGCCA 
GACATTTATA TGCCAATCAA AATTCCAACC CAGTCATTGC TGCAGGATGT TACTGGACAA 
GCAAGAAAAG AGAAAGTGAG GTTACCTTAT TACATGATGA GCTCCAAGCC CAAATCTCAG 
CCTTATGCCA AGGTGGAAGA TCCTGTTGGG GGAAGAGTGA ATACATCCTC CATCGCATCT 
GCTGCTATGT ATAACCCAAA CGCATCCCCT TTCGGGTTCC ACCTTCTCCC ACCATCTGTG 
AAGTTTGGAG TGCTTAAAGA AGGACACACC TATGCGACCA TTGTGAAACT CAAGAATGTC 
GGCGTGGACT TCTGCAGGTT TAGAGTGAAG CAGCCCCCAC CCAGCACTGG ACTGAAAGTG 
ACATATAAAC CTGGGCCTGT GGCTGCTGGT TTGCAAGCAG AGCTAAAGGT AGAGCTTTTT 
GCCATGGCTG TGGGTGAAGA CGGTGCCAAG GGCTCAGCAC ACATCTCCCA CAATATCGAA 
ATCATGACGG AGCATGATGT TCTGTTCTTA CCTGTGGAAG CAACAGTTTT AACAAGCAGT 
AACTATGACA ATCGACCAAA AAATCTTCCC CAGGGAAAAG AAAATCCCAT GGTCTTCAGG 
ACTTCCACAA TCTCCTCCTC TTCACTTGGA GTCGTCATGT CTCAAAAGGC CACTCATCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083168.3
CDS	76..7038
Translation	MAPKKEKPTGSANYKIWEPSLIAAHLNQNDWKASIAFVVGNRVEDDLLIHALDLAVRLPQRKLFSIVSWEDILQQMDEIQSLAESASAKKGKKPTSVNLPLHYEVFLAAKIIMESGEKLTLPLIGKLLKCQLLHIKSKDQQRRENEKKMVEERTKSEKDKGKGKSPKEKKVASAKPGKGKSKDQPEATVTVRKTTQLKRRGEDDEAKSYIDDEPDDGAQYYIIVVGFNNPQLLAIMTELGIPITSVIKISSENYEPLQTHLAAVRQQQEAVLQPEDIEAEKLKRKNSIKELEVFWKYLEPILNNEKLEIHLFDVARLQYMVKATYFPSDWSDNEQMLALGTEIFENIACLMYDSLDWKRQHHHYLQSMQLINVPQVVSEKTVLEAITIPEPPPSTAPAPTGKKKAQYEESHAPPTVAFIITTEVDMRYYNDLLNPIPEEFISVSLILHCMVEQVVATEEDLIPPSLVEPAPRADGLDYRIAAHIVSILPSLCLSEKEKKNLREIFLTEGESESKALPKGPLLLNYHDAHAHKKYALKDQKNFDPVQVEQEMQSKLPLWEFLQFPLPPPWNSTKRLATIHELMHFCTNEVLSWNEVERAFKVFTFESLKLSEVDEEGRLKPTETTSDTDVENFNIPWDNPARFAKLIRQRYIHRMSMQKAPPVVVEIENTERTLFVNKNFAKAEQDAQGDENSPNSDEPDAISVTGSTSNSTKPWNSSNRQFSEKETSGSMWPQPESMDQTMDTEIKDDAATKDDSPEKKPKKMVVEADIEDIKKTQQRSLMDWSFTEYFQPKVLLQVLQEAHQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLQCEHWNIALHSNVGFRNYLELVAKSIEDWVTQEEAKYQEAKMAEELNRIRIELELKATVKTSASKIPGPKRSKTNKVSSKTELSDQEKDKEKEKDKIPFVLEGSLKAWKEEQERLAEEERLKEEKKAEKKGKDTGKKKGKDKADKDDAKALKKKSSSKEKPKEEPAKTLEVIEETAPLPVPEVVYPFRGYNMGDIPIQISGTNYYLYPSDGGQIEVEKTRFERGSTFIKVKVKKDKHNFIIHLKDPKEIVKKEKEEKNSEEEEEEEEEKEEVEEKKPKEGEEEKVKQKVEMKRAQIEKEAVSKFGSFSATLENGICLSISYYGSNGMAPEVINSELEAMMNIPSAMTATVVPAVVTVPQGKGKAKPKGKEKHKDSIKEEELPKEEEKKNHIQEEVEPEIVIQESPPYVPTFQNLNVSCPSGLLLTFIGQESTDYSIVDEEPTRNLMIRQSYPQRLKHYEFYKAVMPPLEQEASRVVTSQGTVIKYMLDGSTQILFADGAVSSSPDSGPVYTSPELPTSPHNGDLVDSASQPKSETGPEIIITKKGKGHKNQTVANKSETHDIIPEVPPPTPVESHIGTWFTTTPDGRRIGTKGLEKIEDLKPYLFFQATDPINGTVMTTREDKVIIVEKKDGTRVVDHADGTRITTFYQVYEDHITPSNDEETTEGPRTVTRQVKCMRIESSHYATIITNCEDSSCCATFGDGTSIIAKPQGSYQVLPPNTGCLYIDKDCSATYCHESSNNLYHPFQKREQLRASRYIMKHTSEVICEVQDPEGNTFQVMADGSVSTTLPKKKLEDDFNVQMEGYESLSSLHLEKNHMQIYGEHVPRFFVVYTDGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEYPGALSITVLRPFHEASQWIMKKELDTIVPPNLQSRSWERFPSVEKKTPGPPFGTQIWKGLSIGSKQLTNIPAPILEGPKVLQMRQFIQHEVIKNEVKLKLQISLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHMTKVAAHLTVLFKQSMASAPKCSPDSYSKEFLEKKWRSLSQGTSWKEKLEQQRNNIKKTQSYLMQIKTKEVTPYFKSELSSLFKSKYDYLEKFSKSLPPFVKKNEAKLMTAVPDLYSDSTLTVDTEKEASNTHPLLNQEVAENIQESPREKETEYVNKSLQTSSSQNQYENVTISPKESVYQSQTEIETKDTKESAIQNFTEKFKKYTKKSASQNEIEDLIKSTKESVSQRQTENVTRPPTEEPDIYMPIKIPTQSLLQDVTGQARKEKVRLPYYMMSSKPKSQPYAKVEDPVGGRVNTSSIASAAMYNPNASPFGFHLLPPSVKFGVLKEGHTYATIVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFAMAVGEDGAKGSAHISHNIEIMTEHDVLFLPVEATVLTSSNYDNRPKNLPQGKENPMVFRTSTISSSSLGVVMSQKATHH

Target ORF information:

RefSeq Version	NM_028892.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 17 (Spag17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028892.4

ORF Insert Sequence:

ATGGCACCCA AGAAGGAGAA ACCTACCGGG AGCGCAAACT ATAAGATATG GGAACCTTCC 
CTCATAGCTG CACACTTGAA CCAGAATGAT TGGAAGGCCT CCATCGCCTT CGTGGTTGGG 
AACCGAGTTG AGGATGATCT TCTCATTCAT GCCCTTGACC TGGCTGTTCG ACTCCCTCAG 
CGGAAACTCT TCAGTATTGT GTCATGGGAA GACATTCTGC AGCAGATGGA TGAAATACAG 
TCACTTGCTG AAAGTGCTTC AGCTAAAAAG GGGAAAAAGC CCACATCTGT CAATTTACCG 
TTACATTACG AGGTGTTCCT GGCAGCAAAG ATAATCATGG AGAGTGGAGA GAAACTAACC 
TTACCATTGA TAGGGAAACT CTTGAAGTGT CAACTTCTCC ATATTAAATC CAAGGACCAG 
CAGAGACGAG AAAATGAAAA GAAGATGGTA GAAGAAAGAA CAAAATCAGA AAAAGACAAA 
GGAAAAGGGA AGTCTCCAAA GGAGAAGAAA GTTGCCAGTG CCAAGCCTGG GAAGGGAAAG 
AGCAAGGACC AGCCTGAGGC CACTGTAACA GTGAGGAAGA CCACACAGTT AAAACGGAGG 
GGAGAAGATG ACGAGGCCAA ATCCTATATC GATGATGAGC CAGACGATGG TGCCCAATAT 
TACATTATTG TTGTGGGCTT CAACAATCCT CAGCTATTGG CCATTATGAC TGAGCTTGGC 
ATTCCTATAA CCAGTGTGAT TAAAATATCT TCAGAGAACT ATGAACCTCT ACAGACACAC 
CTGGCAGCAG TCAGGCAGCA ACAGGAAGCT GTCCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA GAAAAAATTC CATCAAGGAA CTTGAGGTTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACT TGAAATACAT CTCTTTGATG TTGCTCGTCT CCAGTACATG 
GTCAAGGCAA CATATTTTCC TTCTGATTGG TCAGACAATG AACAGATGCT TGCATTGGGC 
ACCGAGATCT TTGAAAACAT TGCCTGTTTG ATGTACGATA GCCTGGACTG GAAGAGGCAA 
CATCATCACT ATTTGCAAAG CATGCAGCTC ATCAATGTCC CACAGGTGGT CAGTGAGAAG 
ACCGTGCTAG AGGCAATCAC AATCCCAGAG CCTCCACCAT CAACTGCTCC AGCCCCCACT 
GGGAAGAAGA AAGCACAGTA TGAGGAGTCG CATGCTCCAC CAACAGTGGC TTTTATCATC 
ACAACTGAAG TAGACATGCG GTATTACAAT GACCTGCTGA ATCCAATTCC AGAGGAATTT 
ATTTCTGTGT CCTTGATACT GCACTGCATG GTGGAACAGG TTGTAGCAAC TGAAGAAGAT 
CTCATCCCGC CAAGTTTGGT GGAGCCAGCC CCCAGAGCAG ACGGGTTAGA CTACAGAATC 
GCAGCTCACA TTGTGTCGAT CCTGCCTTCA CTCTGCCTCT CCGAAAAGGA AAAGAAGAAT 
CTTCGTGAGA TATTCTTAAC TGAAGGAGAG AGTGAAAGCA AAGCCCTGCC CAAAGGTCCC 
CTCCTACTGA ACTACCACGA TGCGCATGCC CACAAGAAGT ATGCACTGAA GGACCAAAAG 
AATTTTGACC CGGTTCAAGT TGAGCAGGAG ATGCAGTCCA AGCTGCCACT GTGGGAATTC 
CTTCAGTTCC CTCTCCCCCC TCCATGGAAC AGCACTAAAC GACTCGCCAC TATTCACGAG 
CTTATGCATT TCTGTACGAA CGAAGTTCTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTATTTACTT TTGAGAGCCT GAAGCTCTCA GAGGTGGATG AGGAAGGGAG GCTCAAGCCC 
ACAGAAACGA CCAGTGACAC AGATGTTGAA AATTTCAACA TTCCTTGGGA CAATCCTGCC 
AGGTTCGCTA AGCTGATAAG GCAGCGGTAC ATTCACAGGA TGAGCATGCA GAAGGCCCCG 
CCAGTAGTAG TAGAGATTGA AAACACTGAG AGAACACTAT TTGTGAACAA GAATTTTGCC 
AAAGCTGAGC AAGATGCACA AGGAGATGAA AATTCCCCAA ATTCTGACGA GCCTGATGCT 
ATTAGTGTGA CGGGTTCTAC TTCCAACTCC ACAAAACCCT GGAATTCTAG TAATAGACAG 
TTCTCAGAAA AAGAGACCAG TGGGTCCATG TGGCCCCAGC CAGAGTCCAT GGATCAGACC 
ATGGACACTG AGATCAAAGA TGACGCAGCC ACAAAGGATG ATTCTCCTGA AAAAAAACCC 
AAGAAGATGG TGGTGGAAGC CGACATAGAG GACATTAAAA AAACGCAACA GCGCAGTTTA 
ATGGACTGGA GCTTTACTGA ATATTTTCAG CCAAAAGTCC TGCTTCAGGT TCTTCAGGAG 
GCTCACCAGC AATACCGATG TGTTGACTCT TACTACCACA CGCAGGATAA CTCTTTACTT 
CTGGTTTTTC ATAATCCAAT GAATCTACAG CGTCTGCAAT GTGAACATTG GAATATTGCT 
CTTCACTCCA ACGTCGGATT CAGGAATTAC TTAGAACTTG TTGCAAAATC TATTGAAGAC 
TGGGTCACAC AGGAAGAAGC GAAGTACCAG GAAGCCAAAA TGGCTGAGGA GCTCAACAGG 
ATCAGGATTG AGTTGGAACT GAAAGCCACT GTCAAAACTT CTGCTAGCAA AATTCCTGGC 
CCCAAAAGAT CTAAAACTAA CAAAGTATCC AGCAAAACAG AGCTATCAGA CCAAGAAAAA 
GATAAAGAAA AGGAAAAAGA CAAGATCCCC TTCGTATTAG AAGGCTCTCT CAAGGCATGG 
AAGGAAGAAC AAGAGAGGTT GGCTGAGGAG GAACGCCTGA AGGAGGAAAA GAAAGCAGAG 
AAGAAGGGAA AGGATACAGG GAAAAAGAAA GGCAAGGATA AGGCCGACAA AGACGATGCT 
AAGGCTTTGA AGAAAAAGTC TTCTTCCAAG GAGAAGCCCA AAGAAGAGCC AGCAAAGACG 
CTGGAAGTGA TAGAGGAAAC CGCCCCACTT CCGGTACCGG AAGTCGTTTA TCCGTTCCGA 
GGATACAATA TGGGAGACAT ACCTATCCAA ATCTCAGGAA CAAATTACTA TCTGTATCCT 
TCTGATGGTG GGCAGATTGA AGTGGAAAAG ACCAGGTTTG AACGAGGGTC AACTTTCATC 
AAAGTCAAAG TAAAAAAAGA TAAACACAAT TTTATAATTC ACCTGAAAGA CCCAAAGGAA 
ATTGTGAAAA AGGAAAAGGA AGAGAAGAAC TCAGAAGAGG AGGAGGAGGA GGAAGAGGAG 
AAGGAGGAGG TAGAAGAGAA GAAGCCGAAG GAGGGGGAAG AGGAAAAGGT TAAACAGAAA 
GTGGAAATGA AAAGAGCACA GATAGAGAAG GAAGCTGTCA GCAAGTTTGG ATCTTTTTCT 
GCCACCTTGG AAAATGGAAT CTGCCTCTCG ATAAGTTACT ATGGATCAAA TGGAATGGCA 
CCAGAGGTTA TAAATTCTGA GTTGGAAGCA ATGATGAATA TTCCTTCAGC TATGACAGCA 
ACAGTGGTTC CTGCTGTAGT GACTGTTCCC CAAGGCAAAG GGAAAGCTAA GCCGAAAGGC 
AAAGAAAAAC ACAAAGATTC CATTAAAGAA GAGGAACTTC CAAAAGAAGA AGAGAAGAAG 
AATCACATAC AGGAAGAAGT AGAACCAGAG ATCGTTATAC AGGAATCACC ACCCTATGTA 
CCCACCTTCC AAAACTTAAA TGTATCTTGC CCCAGTGGGC TCCTGCTGAC CTTCATTGGG 
CAAGAATCTA CAGATTATTC CATCGTAGAT GAAGAACCCA CCCGGAACCT CATGATCCGC 
CAGAGCTACC CCCAGCGGTT GAAGCACTAT GAGTTCTATA AAGCAGTGAT GCCACCCCTG 
GAGCAGGAGG CATCAAGAGT TGTCACCAGC CAGGGCACCG TTATCAAGTA TATGCTGGAT 
GGATCCACAC AGATTCTCTT TGCAGATGGT GCTGTGAGCA GCAGCCCTGA TTCGGGTCCT 
GTTTACACAT CTCCTGAATT GCCTACAAGC CCCCATAATG GAGATTTGGT GGACAGTGCC 
TCTCAGCCCA AATCAGAAAC AGGACCTGAG ATCATCATTA CTAAGAAAGG GAAAGGTCAC 
AAAAATCAAA CAGTGGCAAA TAAGAGTGAA ACCCATGATA TTATCCCAGA GGTACCTCCA 
CCAACTCCCG TAGAGTCCCA CATTGGCACC TGGTTTACCA CAACACCTGA TGGAAGGAGG 
ATTGGCACCA AAGGACTAGA GAAAATTGAG GACTTGAAAC CATATTTGTT CTTCCAGGCT 
ACAGATCCCA TCAATGGAAC GGTCATGACT ACTCGAGAAG ACAAGGTGAT CATTGTTGAG 
AAGAAAGACG GGACTCGGGT TGTGGACCAT GCTGATGGTA CCAGAATCAC AACCTTTTAC 
CAAGTGTATG AAGATCATAT TACTCCCTCA AATGATGAAG AAACAACTGA AGGTCCAAGG 
ACTGTCACTA GACAGGTGAA GTGTATGCGG ATAGAAAGCT CACACTATGC CACTATTATC 
ACCAACTGCG AGGACAGTAG CTGCTGTGCC ACCTTTGGGG ATGGAACATC TATTATTGCA 
AAGCCACAGG GGTCATACCA GGTGCTACCT CCAAACACAG GCTGCCTTTA TATTGATAAA 
GACTGCTCAG CTACATACTG CCATGAATCA AGCAATAATT TGTACCATCC CTTCCAGAAG 
CGTGAGCAGC TGAGAGCCAG CAGGTATATC ATGAAGCACA CCTCAGAGGT GATCTGTGAG 
GTCCAGGATC CTGAGGGGAA CACCTTTCAG GTCATGGCTG ATGGCTCTGT ATCAACAACA 
TTACCTAAAA AGAAACTAGA AGATGACTTC AATGTACAGA TGGAGGGCTA CGAAAGCTTA 
TCGTCACTTC ACCTTGAAAA GAATCACATG CAGATCTATG GTGAACATGT TCCCAGATTT 
TTTGTTGTCT ATACTGATGG ATCGGGAGTG GAACTTCTCC GAGACAGTGA CATAGAAGAA 
TACTTATCCT TGGCGTATGG AGAGTCAACG ACTGTGGTTC TCCAGGAACC AGTACAGGAA 
TACCCAGGTG CACTGAGCAT CACAGTCCTT CGCCCTTTCC ATGAAGCATC ACAATGGATC 
ATGAAGAAGG AATTAGACAC CATAGTTCCT CCAAATCTCC AGTCAAGGTC ATGGGAAAGG 
TTTCCTTCAG TTGAGAAAAA GACTCCAGGA CCACCATTTG GCACTCAAAT TTGGAAAGGC 
CTCTCTATTG GGTCTAAGCA GCTGACAAAC ATCCCAGCCC CTATACTCGA AGGACCCAAA 
GTGCTACAGA TGCGTCAGTT CATTCAGCAT GAAGTCATAA AGAATGAGGT GAAACTAAAG 
CTACAGATTT CCCTTAAGGA CTATATAAAC CACATCCTGA AGAAAGAAGA TGAGCTACAG 
GAGATGACAG TCAAGGATTC CAGAACCGAG GAGGAGAGGG GCAATGCTGC AGATCTCCTC 
AAGCTGGTTA TGTCTTTCCC GAAAATGGAG GAAACTACAA AAAGTCATAT GACTAAAGTT 
GCAGCCCACC TAACAGTTTT ATTCAAGCAA TCTATGGCTT CGGCTCCAAA ATGTTCACCA 
GACAGCTACT CTAAAGAATT CCTTGAAAAG AAGTGGAGGA GTCTGTCACA GGGAACGAGC 
TGGAAAGAAA AGCTTGAGCA GCAGAGGAAC AATATCAAGA AAACACAAAG CTACCTAATG 
CAAATTAAGA CAAAAGAAGT AACACCTTAT TTTAAGTCCG AATTGAGCTC GTTATTTAAG 
TCAAAGTATG ATTATCTGGA GAAGTTTTCC AAAAGCCTGC CTCCTTTTGT GAAGAAAAAT 
GAGGCCAAAC TCATGACAGC TGTTCCGGAT CTCTACTCGG ATTCTACACT GACCGTAGAT 
ACAGAAAAAG AAGCCTCAAA TACACATCCC CTTCTAAACC AGGAGGTTGC TGAGAATATC 
CAAGAGTCTC CTAGGGAAAA AGAAACGGAA TACGTAAACA AATCTCTCCA GACCTCTTCT 
TCCCAAAACC AATATGAAAA TGTAACAATA TCTCCCAAGG AGTCTGTTTA TCAAAGTCAA 
ACTGAAATTG AAACCAAAGA TACCAAGGAA TCTGCTATCC AAAACTTCAC TGAAAAGTTT 
AAGAAATATA CCAAGAAGTC TGCTAGCCAA AACGAAATTG AAGATCTAAT AAAATCTACC 
AAGGAGTCTG TTAGCCAAAG ACAAACTGAA AATGTCACCA GACCTCCTAC TGAAGAGCCA 
GACATTTATA TGCCAATCAA AATTCCAACC CAGTCATTGC TGCAGGATGT TACTGGACAA 
GCAAGAAAAG AGAAAGTGAG GTTACCTTAT TACATGATGA GCTCCAAGCC CAAATCTCAG 
CCTTATGCCA AGGTGGAAGA TCCTGTTGGG GGAAGAGTGA ATACATCCTC CATCGCATCT 
GCTGCTATGT ATAACCCAAA CGCATCCCCT TTCGGGTTCC ACCTTCTCCC ACCATCTGTG 
AAGTTTGGAG TGCTTAAAGA AGGACACACC TATGCGACCA TTGTGAAACT CAAGAATGTC 
GGCGTGGACT TCTGCAGGTT TAGAGTGAAG CAGCCCCCAC CCAGCACTGG ACTGAAAGTG 
ACATATAAAC CTGGGCCTGT GGCTGCTGGT TTGCAAGCAG AGCTAAAGGT AGAGCTTTTT 
GCCATGGCTG TGGGTGAAGA CGGTGCCAAG GGCTCAGCAC ACATCTCCCA CAATATCGAA 
ATCATGACGG AGCATGATGT TCTGTTCTTA CCTGTGGAAG CAACAGTTTT AACAAGCAGT 
AACTATGACA ATCGACCAAA AAATCTTCCC CAGGGAAAAG AAAATCCCAT GGTCTTCAGG 
ACTTCCACAA TCTCCTCCTC TTCACTTGGA GTCGTCATGT CTCAAAAGGC CACTCATCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse.
Developmental biology423(2)170-188(2017 03)
Stauber M,Weidemann M,Dittrich-Breiholz O,Lobschat K,Alten L,Mai M,Beckers A,Kracht M,Gossler A

Spag17 deficiency results in skeletal malformations and bone abnormalities.
PloS one10(5)e0125936(2015)
Teves ME,Sundaresan G,Cohen DJ,Hyzy SL,Kajan I,Maczis M,Zhang Z,Costanzo RM,Zweit J,Schwartz Z,Boyan BD,Strauss JF 3rd

Sperm-associated antigen-17 gene is essential for motile cilia function and neonatal survival.
American journal of respiratory cell and molecular biology48(6)765-72(2013 Jun)
Teves ME,Zhang Z,Costanzo RM,Henderson SC,Corwin FD,Zweit J,Sundaresan G,Subler M,Salloum FN,Rubin BK,Strauss JF 3rd

Dissecting the axoneme interactome: the mammalian orthologue of Chlamydomonas PF6 interacts with sperm-associated antigen 6, the mammalian orthologue of Chlamydomonas PF16.
Molecular & cellular proteomics : MCP4(7)914-23(2005 Jul)
Zhang Z,Jones BH,Tang W,Moss SB,Wei Z,Ho C,Pollack M,Horowitz E,Bennett J,Baker ME,Strauss JF 3rd

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Spag17 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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