Chic2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chic2
Entrez Gene ID	74277
Full Name	cysteine-rich hydrophobic domain 2
Synonyms	1700081B18Rik,4930502K01Rik,BTL
General protein information	Preferred Names cysteine-rich hydrophobic domain 2 Names cysteine-rich hydrophobic domain-containing protein 2 cysteine-rich hydrophobic domain 2 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_028850.5	NP_083126.1	cysteine-rich hydrophobic domain-containing protein 2

Pathways from BioSystems

Homologies

	CHIC2	XP_001089417.1
Canis lupus familiaris (dog)	CHIC2	XP_005628250.1
Rattus norvegicus (Norway rat)	Chic2	NP_001099206.1
Xenopus tropicalis (tropical clawed frog)	chic2	NP_001107383.1
Bos taurus (cattle)	CHIC2	NP_001071516.1
Gallus gallus (chicken)	CHIC2	XP_420705.1
Homo sapiens (human)	CHIC2	NP_036242.1
Pan troglodytes (chimpanzee)	CHIC2	XP_003950405.1
Danio rerio (zebrafish)	chic2	NP_957414.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001278	XP_321873.3
Mus musculus (house mouse)	Chic2	NP_083126.1
Drosophila melanogaster (fruit fly)	CG5938	NP_001097947.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

A new family of small, palmitoylated, membrane-associated proteins, characterized by the presence of a cysteine-rich hydrophobic motif.
Cools J, Mentens N, Marynen P
FEBS letters492(3)204-9(2001 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chic2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chic2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06230	NM_028850.5 Latest version!	Mus musculus cysteine-rich hydrophobic domain 2 (Chic2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chic2 ( NM_028850.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083126.1 Mus musculus cysteine-rich hydrophobic domain 2 (Chic2), mRNA.

Price & Availability↑

CloneID	OMu06230
Clone ID Related Accession (Same CDS sequence)	NM_028850.5
Accession Version	NM_028850.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	cysteine-rich hydrophobic domain-containing protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161192.12, AK006962.1, AK015681.1 and CF744991.1. On May 9, 2015 this sequence version replaced NM_028850.4. ##Evidence-Data-START## Transcript exon combination :: BB609895.1, AK006962.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATT TCGATGAAAT CTACGAGGAG GAGGAGGACG AGGAGCGGGC CCTGGAGGAG 
CAGCTGCTCA AGTACTCGCC CGACCCGGTG GTGGTCCGCG GCTCCGGTCA CGTCACCGTA 
TTTGGACTGA GCAACAAATT TGAATCAGAA TTCCCTTCTT CATTAACTGG AAAAGTAGCT 
CCTGAAGAAT TTAAAGCCAG CATCAACAGA GTTAACAGCT GTCTCAGGAA GAATCTTCCT 
GTTAATGTGC GGTGGTTGCT TTGTGGCTGC CTGTGCTGCT GCTGCACGTT AGGGTGCAGT 
ATGTGGCCAG TTATTTGCCT CAGTAAAAGA ACACGAAGAT CGATTGAGAA GTTATTAGAA 
TGGGAAAACA ATAGGTTATA CCACAAGCTG TGCTTGCACT GGAGACTGAG CAAAAGGAAA 
TGTGAAACGA ACAACATGAT GGAATATGTC ATCCTCATAG AATTTTTACC AAAGACACCG 
ATTTTTCGAC CAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083126.1
CDS	344..841
Translation	MADFDEIYEEEEDEERALEEQLLKYSPDPVVVRGSGHVTVFGLSNKFESEFPSSLTGKVAPEEFKASINRVNSCLRKNLPVNVRWLLCGCLCCCCTLGCSMWPVICLSKRTRRSIEKLLEWENNRLYHKLCLHWRLSKRKCETNNMMEYVILIEFLPKTPIFRPD

Target ORF information:

RefSeq Version	NM_028850.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteine-rich hydrophobic domain 2 (Chic2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028850.5

ORF Insert Sequence:

ATGGCGGATT TCGATGAAAT CTACGAGGAG GAGGAGGACG AGGAGCGGGC CCTGGAGGAG 
CAGCTGCTCA AGTACTCGCC CGACCCGGTG GTGGTCCGCG GCTCCGGTCA CGTCACCGTA 
TTTGGACTGA GCAACAAATT TGAATCAGAA TTCCCTTCTT CATTAACTGG AAAAGTAGCT 
CCTGAAGAAT TTAAAGCCAG CATCAACAGA GTTAACAGCT GTCTCAGGAA GAATCTTCCT 
GTTAATGTGC GGTGGTTGCT TTGTGGCTGC CTGTGCTGCT GCTGCACGTT AGGGTGCAGT 
ATGTGGCCAG TTATTTGCCT CAGTAAAAGA ACACGAAGAT CGATTGAGAA GTTATTAGAA 
TGGGAAAACA ATAGGTTATA CCACAAGCTG TGCTTGCACT GGAGACTGAG CAAAAGGAAA 
TGTGAAACGA ACAACATGAT GGAATATGTC ATCCTCATAG AATTTTTACC AAAGACACCG 
ATTTTTCGAC CAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

A new family of small, palmitoylated, membrane-associated proteins, characterized by the presence of a cysteine-rich hydrophobic motif.
FEBS letters492(3)204-9(2001 Mar)
Cools J,Mentens N,Marynen P

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Chic2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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