Usp20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp20
Entrez Gene ID	74270
Full Name	ubiquitin specific peptidase 20
Synonyms	1700055M05Rik,AI467231,Vdu2
General protein information	Preferred Names ubiquitin specific peptidase 20 Names ubiquitin carboxyl-terminal hydrolase 20 VHL-interacting deubiquitinating enzyme 2 deubiquitinating enzyme 20 ubiquitin specific protease 20 ubiquitin thioesterase 20 ubiquitin thiolesterase 20 ubiquitin-specific-processing protease 20
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 B

mRNA and Protein(s)

mRNA	Protein	Name
XM_017319296.1	XP_017174785.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
XM_017319297.1	XP_017174786.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
NM_028846.5	NP_083122.1	ubiquitin carboxyl-terminal hydrolase 20
XM_006498392.1	XP_006498455.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
XM_030252135.1	XP_030107995.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Usp20	NP_083122.1
	USP20	XP_001107144.1
Canis lupus familiaris (dog)	USP20	XP_537816.3
Bos taurus (cattle)	USP20	NP_001098803.1
Gallus gallus (chicken)	USP20	XP_004945924.1
Danio rerio (zebrafish)	usp20	NP_957281.2
Drosophila melanogaster (fruit fly)	CG8494	NP_610943.2
Xenopus tropicalis (tropical clawed frog)	usp20	NP_001090641.1
Homo sapiens (human)	USP20	NP_001008563.2
Pan troglodytes (chimpanzee)	USP20	XP_520313.2
Rattus norvegicus (Norway rat)	Usp20	NP_001101297.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003821	XP_310382.7

Gene Ontology
Bibliography

Related articles in PubMed

Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions of ?-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor ?B (NF?B) Activation.
Jean-Charles PY, Zhang L, Wu JH, Han SO, Brian L, Freedman NJ, Shenoy SK
The Journal of biological chemistry291(14)7450-64(2016 Apr)

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Lumayag S, Haldin CE, Corbett NJ, Wahlin KJ, Cowan C, Turturro S, Larsen PE, Kovacs B, Witmer PD, Valle D, Zack DJ, Nicholson DA, Xu S
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

that pro- and anti-inflammatory activities of beta-arrestin2 are determined by beta-arrestin2 ubiquitination and that changes in USP20 expression and/or activity can therefore regulate inflammatory responses
Title: Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions of ?-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor ?B (NF?B) Activation.

The following Usp20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06222	NM_028846.5 Latest version!	Mus musculus ubiquitin specific peptidase 20 (Usp20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu06222	XM_006498392.1 Latest version!	Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu06222	XM_030252135.1 Latest version!	Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu80438	XM_017319296.1 Latest version!	Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu80438	XM_017319297.1 Latest version!	Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp20 ( NM_028846.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083122.1 Mus musculus ubiquitin specific peptidase 20 (Usp20), mRNA.

Price & Availability↑

CloneID	OMu06222
Clone ID Related Accession (Same CDS sequence)	NM_028846.5 , XM_006498392.1 , XM_030252135.1
Accession Version	NM_028846.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY251374.1, AK173083.1, AK047242.1 and AK054279.1. On Apr 22, 2010 this sequence version replaced NM_028846.4. ##Evidence-Data-START## Transcript exon combination :: AK054279.1, AF449715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTATCC CACCCAACAA ATACCACTAC ATCGATGACC TGGTGGTCAT CCTGCCGCAG 
AGCGTCTGGG AGCACCTGTA CAGCAGGTTT GGGGGCGGCC CTGCTGTGAA CCATCTGTAT 
GTGTGCTCCA TCTGCCAGGT GGAGATTGAG GCCCTGGCCA AGCGCAGGAG GGTGGAGATT 
GACACTTTCA TCAAGCTGAA CAAGGCATTC CAAGCTGAGG AGTCTCCCGC GGTCATCTAC 
TGTATCAGCA TGCACTGGTT CCGAGAGTGG GAGGCCTTCG TCAAGGGGAA GGACAGTGAG 
CCTCCCGGGC CCATCGACAA CAGCAGGATT GCCCAGGTCA AAGGCAGTGG CCATATCCAG 
CTAAAGCAGG GGGCTGACTG TGGCCAGATT TCGGAGGAGA CCTGGACCTA TCTGAGCAGT 
CTGTATGGGG GCGGCCCTGA GATTGCCATC CGGCAGAGTG TGGCCCAGCT CCCAGACCCA 
GAAAGCTTGC ACGGAGAGCA GAAGATTGAG GCTGAGACCC GGGCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083122.1
CDS	236..2986
Translation	MGDARDLCPHLDCIGEVTKEDLLLKSKGTCQSCGVAGPNLWACLQVTCPYVGCGESFADHSSIHAQVKKHNLTVNLTTFRVWCYACEREVFLEQRLAVHLASSSARLSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRSTSPCQTPEPDNEAHIRSSSRPCSPVHHHHEGHSKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDVFNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTSQVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCSHGGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRVEIDTFIKLNKAFQAEESPAVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGSGHIQLKQGADCGQISEETWTYLSSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETRAL

Target ORF information:

RefSeq Version	NM_028846.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 20 (Usp20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028846.5

ORF Insert Sequence:

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTATCC CACCCAACAA ATACCACTAC ATCGATGACC TGGTGGTCAT CCTGCCGCAG 
AGCGTCTGGG AGCACCTGTA CAGCAGGTTT GGGGGCGGCC CTGCTGTGAA CCATCTGTAT 
GTGTGCTCCA TCTGCCAGGT GGAGATTGAG GCCCTGGCCA AGCGCAGGAG GGTGGAGATT 
GACACTTTCA TCAAGCTGAA CAAGGCATTC CAAGCTGAGG AGTCTCCCGC GGTCATCTAC 
TGTATCAGCA TGCACTGGTT CCGAGAGTGG GAGGCCTTCG TCAAGGGGAA GGACAGTGAG 
CCTCCCGGGC CCATCGACAA CAGCAGGATT GCCCAGGTCA AAGGCAGTGG CCATATCCAG 
CTAAAGCAGG GGGCTGACTG TGGCCAGATT TCGGAGGAGA CCTGGACCTA TCTGAGCAGT 
CTGTATGGGG GCGGCCCTGA GATTGCCATC CGGCAGAGTG TGGCCCAGCT CCCAGACCCA 
GAAAGCTTGC ACGGAGAGCA GAAGATTGAG GCTGAGACCC GGGCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp20 ( XM_006498392.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498455.1 Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu06222
Clone ID Related Accession (Same CDS sequence)	NM_028846.5 , XM_006498392.1 , XM_030252135.1
Accession Version	XM_006498392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
Comment

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTATCC CACCCAACAA ATACCACTAC ATCGATGACC TGGTGGTCAT CCTGCCGCAG 
AGCGTCTGGG AGCACCTGTA CAGCAGGTTT GGGGGCGGCC CTGCTGTGAA CCATCTGTAT 
GTGTGCTCCA TCTGCCAGGT GGAGATTGAG GCCCTGGCCA AGCGCAGGAG GGTGGAGATT 
GACACTTTCA TCAAGCTGAA CAAGGCATTC CAAGCTGAGG AGTCTCCCGC GGTCATCTAC 
TGTATCAGCA TGCACTGGTT CCGAGAGTGG GAGGCCTTCG TCAAGGGGAA GGACAGTGAG 
CCTCCCGGGC CCATCGACAA CAGCAGGATT GCCCAGGTCA AAGGCAGTGG CCATATCCAG 
CTAAAGCAGG GGGCTGACTG TGGCCAGATT TCGGAGGAGA CCTGGACCTA TCTGAGCAGT 
CTGTATGGGG GCGGCCCTGA GATTGCCATC CGGCAGAGTG TGGCCCAGCT CCCAGACCCA 
GAAAGCTTGC ACGGAGAGCA GAAGATTGAG GCTGAGACCC GGGCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006498455.1

Translation

MGDARDLCPHLDCIGEVTKEDLLLKSKGTCQSCGVAGPNLWACLQVTCPYVGCGESFADHSSIHAQVKKHNLTVNLTTFRVWCYACEREVFLEQRLAVHLASSSARLSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRSTSPCQTPEPDNEAHIRSSSRPCSPVHHHHEGHSKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDVFNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTSQVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCSHGGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRVEIDTFIKLNKAFQAEESPAVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGSGHIQLKQGADCGQISEETWTYLSSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETRAL

Target ORF information:

RefSeq Version	XM_006498392.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498392.1

ORF Insert Sequence:

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTATCC CACCCAACAA ATACCACTAC ATCGATGACC TGGTGGTCAT CCTGCCGCAG 
AGCGTCTGGG AGCACCTGTA CAGCAGGTTT GGGGGCGGCC CTGCTGTGAA CCATCTGTAT 
GTGTGCTCCA TCTGCCAGGT GGAGATTGAG GCCCTGGCCA AGCGCAGGAG GGTGGAGATT 
GACACTTTCA TCAAGCTGAA CAAGGCATTC CAAGCTGAGG AGTCTCCCGC GGTCATCTAC 
TGTATCAGCA TGCACTGGTT CCGAGAGTGG GAGGCCTTCG TCAAGGGGAA GGACAGTGAG 
CCTCCCGGGC CCATCGACAA CAGCAGGATT GCCCAGGTCA AAGGCAGTGG CCATATCCAG 
CTAAAGCAGG GGGCTGACTG TGGCCAGATT TCGGAGGAGA CCTGGACCTA TCTGAGCAGT 
CTGTATGGGG GCGGCCCTGA GATTGCCATC CGGCAGAGTG TGGCCCAGCT CCCAGACCCA 
GAAAGCTTGC ACGGAGAGCA GAAGATTGAG GCTGAGACCC GGGCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp20 ( XM_030252135.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107995.1 Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.

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CloneID	OMu06222
Clone ID Related Accession (Same CDS sequence)	NM_028846.5 , XM_006498392.1 , XM_030252135.1
Accession Version	XM_030252135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTATCC CACCCAACAA ATACCACTAC ATCGATGACC TGGTGGTCAT CCTGCCGCAG 
AGCGTCTGGG AGCACCTGTA CAGCAGGTTT GGGGGCGGCC CTGCTGTGAA CCATCTGTAT 
GTGTGCTCCA TCTGCCAGGT GGAGATTGAG GCCCTGGCCA AGCGCAGGAG GGTGGAGATT 
GACACTTTCA TCAAGCTGAA CAAGGCATTC CAAGCTGAGG AGTCTCCCGC GGTCATCTAC 
TGTATCAGCA TGCACTGGTT CCGAGAGTGG GAGGCCTTCG TCAAGGGGAA GGACAGTGAG 
CCTCCCGGGC CCATCGACAA CAGCAGGATT GCCCAGGTCA AAGGCAGTGG CCATATCCAG 
CTAAAGCAGG GGGCTGACTG TGGCCAGATT TCGGAGGAGA CCTGGACCTA TCTGAGCAGT 
CTGTATGGGG GCGGCCCTGA GATTGCCATC CGGCAGAGTG TGGCCCAGCT CCCAGACCCA 
GAAAGCTTGC ACGGAGAGCA GAAGATTGAG GCTGAGACCC GGGCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107995.1
CDS	265..3015
Translation	MGDARDLCPHLDCIGEVTKEDLLLKSKGTCQSCGVAGPNLWACLQVTCPYVGCGESFADHSSIHAQVKKHNLTVNLTTFRVWCYACEREVFLEQRLAVHLASSSARLSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRSTSPCQTPEPDNEAHIRSSSRPCSPVHHHHEGHSKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDVFNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTSQVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCSHGGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRVEIDTFIKLNKAFQAEESPAVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGSGHIQLKQGADCGQISEETWTYLSSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETRAL

Target ORF information:

RefSeq Version	XM_030252135.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252135.1

ORF Insert Sequence:

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTATCC CACCCAACAA ATACCACTAC ATCGATGACC TGGTGGTCAT CCTGCCGCAG 
AGCGTCTGGG AGCACCTGTA CAGCAGGTTT GGGGGCGGCC CTGCTGTGAA CCATCTGTAT 
GTGTGCTCCA TCTGCCAGGT GGAGATTGAG GCCCTGGCCA AGCGCAGGAG GGTGGAGATT 
GACACTTTCA TCAAGCTGAA CAAGGCATTC CAAGCTGAGG AGTCTCCCGC GGTCATCTAC 
TGTATCAGCA TGCACTGGTT CCGAGAGTGG GAGGCCTTCG TCAAGGGGAA GGACAGTGAG 
CCTCCCGGGC CCATCGACAA CAGCAGGATT GCCCAGGTCA AAGGCAGTGG CCATATCCAG 
CTAAAGCAGG GGGCTGACTG TGGCCAGATT TCGGAGGAGA CCTGGACCTA TCTGAGCAGT 
CTGTATGGGG GCGGCCCTGA GATTGCCATC CGGCAGAGTG TGGCCCAGCT CCCAGACCCA 
GAAAGCTTGC ACGGAGAGCA GAAGATTGAG GCTGAGACCC GGGCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp20 ( XM_017319296.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174785.1 Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80438
Clone ID Related Accession (Same CDS sequence)	XM_017319296.1 , XM_017319297.1
Accession Version	XM_017319296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTGAGC AGTCCGCGCC GGGAAGAGGT ATGAGGGGGG GTGCGGTGGG CCAAGCCCCA 
GCTCCCCCCA TGCTTCTCTT CACAGGTATC CCACCCAACA AATACCACTA CATCGATGAC 
CTGGTGGTCA TCCTGCCGCA GAGCGTCTGG GAGCACCTGT ACAGCAGGTT TGGGGGCGGC 
CCTGCTGTGA ACCATCTGTA TGTGTGCTCC ATCTGCCAGG TGGAGATTGA GGCCCTGGCC 
AAGCGCAGGA GGGTGGAGAT TGACACTTTC ATCAAGCTGA ACAAGGCATT CCAAGCTGAG 
GAGTCTCCCG CGGTCATCTA CTGTATCAGC ATGCACTGGT TCCGAGAGTG GGAGGCCTTC 
GTCAAGGGGA AGGACAGTGA GCCTCCCGGG CCCATCGACA ACAGCAGGAT TGCCCAGGTC 
AAAGGCAGTG GCCATATCCA GCTAAAGCAG GGGGCTGACT GTGGCCAGAT TTCGGAGGAG 
ACCTGGACCT ATCTGAGCAG TCTGTATGGG GGCGGCCCTG AGATTGCCAT CCGGCAGAGT 
GTGGCCCAGC TCCCAGACCC AGAAAGCTTG CACGGAGAGC AGAAGATTGA GGCTGAGACC 
CGGGCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174785.1
CDS	231..3062
Translation	MGDARDLCPHLDCIGEVTKEDLLLKSKGTCQSCGVAGPNLWACLQVTCPYVGCGESFADHSSIHAQVKKHNLTVNLTTFRVWCYACEREVFLEQRLAVHLASSSARLSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRSTSPCQTPEPDNEAHIRSSSRPCSPVHHHHEGHSKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDVFNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTSQVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCSHGGEQSAPGRGMRGGAVGQAPAPPMLLFTGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRVEIDTFIKLNKAFQAEESPAVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGSGHIQLKQGADCGQISEETWTYLSSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETRAL

Target ORF information:

RefSeq Version	XM_017319296.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319296.1

ORF Insert Sequence:

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTGAGC AGTCCGCGCC GGGAAGAGGT ATGAGGGGGG GTGCGGTGGG CCAAGCCCCA 
GCTCCCCCCA TGCTTCTCTT CACAGGTATC CCACCCAACA AATACCACTA CATCGATGAC 
CTGGTGGTCA TCCTGCCGCA GAGCGTCTGG GAGCACCTGT ACAGCAGGTT TGGGGGCGGC 
CCTGCTGTGA ACCATCTGTA TGTGTGCTCC ATCTGCCAGG TGGAGATTGA GGCCCTGGCC 
AAGCGCAGGA GGGTGGAGAT TGACACTTTC ATCAAGCTGA ACAAGGCATT CCAAGCTGAG 
GAGTCTCCCG CGGTCATCTA CTGTATCAGC ATGCACTGGT TCCGAGAGTG GGAGGCCTTC 
GTCAAGGGGA AGGACAGTGA GCCTCCCGGG CCCATCGACA ACAGCAGGAT TGCCCAGGTC 
AAAGGCAGTG GCCATATCCA GCTAAAGCAG GGGGCTGACT GTGGCCAGAT TTCGGAGGAG 
ACCTGGACCT ATCTGAGCAG TCTGTATGGG GGCGGCCCTG AGATTGCCAT CCGGCAGAGT 
GTGGCCCAGC TCCCAGACCC AGAAAGCTTG CACGGAGAGC AGAAGATTGA GGCTGAGACC 
CGGGCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp20 ( XM_017319297.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174786.1 Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X2, mRNA.

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CloneID	OMu80438
Clone ID Related Accession (Same CDS sequence)	XM_017319296.1 , XM_017319297.1
Accession Version	XM_017319297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTGAGC AGTCCGCGCC GGGAAGAGGT ATGAGGGGGG GTGCGGTGGG CCAAGCCCCA 
GCTCCCCCCA TGCTTCTCTT CACAGGTATC CCACCCAACA AATACCACTA CATCGATGAC 
CTGGTGGTCA TCCTGCCGCA GAGCGTCTGG GAGCACCTGT ACAGCAGGTT TGGGGGCGGC 
CCTGCTGTGA ACCATCTGTA TGTGTGCTCC ATCTGCCAGG TGGAGATTGA GGCCCTGGCC 
AAGCGCAGGA GGGTGGAGAT TGACACTTTC ATCAAGCTGA ACAAGGCATT CCAAGCTGAG 
GAGTCTCCCG CGGTCATCTA CTGTATCAGC ATGCACTGGT TCCGAGAGTG GGAGGCCTTC 
GTCAAGGGGA AGGACAGTGA GCCTCCCGGG CCCATCGACA ACAGCAGGAT TGCCCAGGTC 
AAAGGCAGTG GCCATATCCA GCTAAAGCAG GGGGCTGACT GTGGCCAGAT TTCGGAGGAG 
ACCTGGACCT ATCTGAGCAG TCTGTATGGG GGCGGCCCTG AGATTGCCAT CCGGCAGAGT 
GTGGCCCAGC TCCCAGACCC AGAAAGCTTG CACGGAGAGC AGAAGATTGA GGCTGAGACC 
CGGGCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174786.1
CDS	265..3096
Translation	MGDARDLCPHLDCIGEVTKEDLLLKSKGTCQSCGVAGPNLWACLQVTCPYVGCGESFADHSSIHAQVKKHNLTVNLTTFRVWCYACEREVFLEQRLAVHLASSSARLSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRSTSPCQTPEPDNEAHIRSSSRPCSPVHHHHEGHSKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDVFNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTSQVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCSHGGEQSAPGRGMRGGAVGQAPAPPMLLFTGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRVEIDTFIKLNKAFQAEESPAVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGSGHIQLKQGADCGQISEETWTYLSSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETRAL

Target ORF information:

RefSeq Version	XM_017319297.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 20 (Usp20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319297.1

ORF Insert Sequence:

ATGGGGGACG CCAGAGACCT CTGCCCTCAC CTGGACTGCA TAGGGGAGGT GACCAAAGAG 
GATTTGCTGC TTAAATCTAA GGGAACCTGC CAGTCATGTG GGGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCTTAC GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCCCAGGT GAAAAAGCAC AACCTGACTG TGAACCTGAC CACGTTCCGG 
GTCTGGTGCT ACGCCTGTGA GCGTGAAGTC TTTCTGGAGC AGCGTCTGGC AGTCCACCTG 
GCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTCCCA CCCGCTGAAA 
GCTGTCCCTA TTGCTGTGGC TGATGAAGGA GAGTCTGAGT CTGAGGATGA CGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGAAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCGCTCACC CAGTTCTTCC TGGAGTGTGG TGGTCTGGTG 
CGCACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTGTGG 
CACAAGAAGC GGCCAAGCTA TGTGGTCCCC ACCAGCCTGT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTAATG 
GACCAGCTTC ATGAGGAGCT CAAGGAGCCC ATGGTCGCTG CTGTGGCAGC ACTCACCGAT 
GCTCGGGACT CCGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCATCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGG 
CGTGGTGGAG GCAGCTCAAA GGCCGAGATG GAGCTGCTGA TCTCAGATGA GGCGGGCCGA 
GCCATCTCTG AGAAGGAGCG GATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCAGAGC AAGTGGATGA GGATGCAGAT GTGGACACGG CCATGGCGTC CCTTGATGAG 
CAATCCAGAG AGGCCCAGCC ACCATCACCA AGGTCCACCA GCCCCTGCCA GACCCCAGAA 
CCAGACAACG AAGCCCACAT ACGCAGCTCC TCTCGTCCCT GCAGCCCTGT TCACCACCAC 
CACGAGGGCC ATTCCAAGCT GTCCAGCAGC CCTCCTCGTG CAAGCCCTGT GAGGATGGGG 
CCGTCGTATG TGCTCAAGAA AGCCCAGGTG CCAAGCACTG GTGGTCGGAG GCGGAAGGAG 
CAGAGCTACC GCAGTGTCAT CTCAGACGTC TTCAATGGCT CTGTCCTCAG CCTGGTGCAG 
TGTCTTACCT GTGACCGGGT GTCTACCACA GTGGAGACAT TCCAGGACCT GTCATTGCCC 
ATCCCTGGCA AGGAAGATCT AGCCAAACTC CACTCTGCCA TCTACCAGAA TGTACCAGCC 
AAGCCGGGTG CCTGTGGGGA CAGCTATTCC TCCCAGGGCT GGCTGGCCTT CATCGTGGAG 
TACATCAGAC GGTTTGTGGT ATCCTGTACC CCCAGCTGGT TTTGGGGACC TGTTGTCACA 
CTAGAAGACT GCCTTGCTGC TTTCTTTGCT GCTGATGAGC TGAAGGGTGA CAACATGTAC 
AGCTGTGAGC GGTGTAAGAA GCTTCGGAAT GGCGTGAAGT ACTGTAAAGT CCTATGTCTG 
CCTGAGATTC TGTGCGTCCA CCTGAAGCGC TTCCGGCATG AGGTGATGTA CTCCTTCAAG 
GTCAGCAGCC ATGTCTCTTT CCCCCTCGAG GGACTGGACC TACGCCCTTT TCTGGCCAAG 
GAGTGCACGT CCCAGGTCAC CACCTATGAC CTCCTCTCTG TCATCTGTCA CCATGGCACA 
GCAGGCAGTG GGCACTACAT TGCCTACTGT CAGAACGTGA TCAATGGGCA GTGGTACGAA 
TTCGATGACC AGTACGTCAC CGAGGTCCAT GAGACGGTAG TACAGAACGT GGAGGCCTAC 
GTGCTGTTCT ACAGGAAGAG CAGTGAGGAG GCCATGCGTG AGCGGCAGCA GGTAGTGTCC 
CTGGCTGCCA TGCGGGAACC CAGCCTGCTC CGCTTCTACG TGTCCCGAGA ATGGCTCAAC 
AAATTCAACA CCTTTGCCGA GCCAGGGCCC ATCACCAACC ACACCTTCCT GTGCTCCCAT 
GGAGGTGAGC AGTCCGCGCC GGGAAGAGGT ATGAGGGGGG GTGCGGTGGG CCAAGCCCCA 
GCTCCCCCCA TGCTTCTCTT CACAGGTATC CCACCCAACA AATACCACTA CATCGATGAC 
CTGGTGGTCA TCCTGCCGCA GAGCGTCTGG GAGCACCTGT ACAGCAGGTT TGGGGGCGGC 
CCTGCTGTGA ACCATCTGTA TGTGTGCTCC ATCTGCCAGG TGGAGATTGA GGCCCTGGCC 
AAGCGCAGGA GGGTGGAGAT TGACACTTTC ATCAAGCTGA ACAAGGCATT CCAAGCTGAG 
GAGTCTCCCG CGGTCATCTA CTGTATCAGC ATGCACTGGT TCCGAGAGTG GGAGGCCTTC 
GTCAAGGGGA AGGACAGTGA GCCTCCCGGG CCCATCGACA ACAGCAGGAT TGCCCAGGTC 
AAAGGCAGTG GCCATATCCA GCTAAAGCAG GGGGCTGACT GTGGCCAGAT TTCGGAGGAG 
ACCTGGACCT ATCTGAGCAG TCTGTATGGG GGCGGCCCTG AGATTGCCAT CCGGCAGAGT 
GTGGCCCAGC TCCCAGACCC AGAAAGCTTG CACGGAGAGC AGAAGATTGA GGCTGAGACC 
CGGGCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions of ?-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor ?B (NF?B) Activation.
The Journal of biological chemistry291(14)7450-64(2016 Apr)
Jean-Charles PY,Zhang L,Wu JH,Han SO,Brian L,Freedman NJ,Shenoy SK

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)
Lumayag S,Haldin CE,Corbett NJ,Wahlin KJ,Cowan C,Turturro S,Larsen PE,Kovacs B,Witmer PD,Valle D,Zack DJ,Nicholson DA,Xu S

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Usp20 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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