gRNAs
The following Chpf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chpf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu10389 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001001565.2 | |
| Accession Version | NM_001001565.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | chondroitin sulfate synthase 2 isoform b | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115011.27, BB651806.1, BC060598.1 and BC071273.1. On Aug 29, 2009 this sequence version replaced NM_001001565.1. Transcript Variant: This variant (2) includes an alternate exon in the coding region and uses a downstream AUG compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC057050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCGGTGG TGGCACTGGG CGAAGAGAGG CCCATCGGAC ACCTGCACCT GGCGCTGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001001565.1 |
| CDS | 996..2834 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001001565.2 |
1 | ATGGCGGTGG TGGCACTGGG CGAAGAGAGG CCCATCGGAC ACCTGCACCT GGCGCTGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu10391 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001001566.3 | |
| Accession Version | NM_001001566.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | chondroitin sulfate synthase 2 isoform a | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115011.27 and BC071273.1. On Aug 29, 2009 this sequence version replaced NM_001001566.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC071273.1, SRR1660817.245225.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCGGGCGT CGCTGCTGCT GTCCGTGCTG CGGCCCGCGG GGCCCGTGGC CGTGGGCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001001566.1 |
| CDS | 367..2691 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001001566.3 |
1 | ATGCGGGCGT CGCTGCTGCT GTCCGTGCTG CGGCCCGCGG GGCCCGTGGC CGTGGGCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Chondroitin sulfate synthase 1 (Chsy1) is required for bone development and digit patterning. |
Parkin interacts with Klokin1 for mitochondrial import and maintenance of membrane potential. |
Gene network and pathway analysis of mice with conditional ablation of Dicer in post-mitotic neurons. |
Chondroitin sulfate synthase-2 is necessary for chain extension of chondroitin sulfate but not critical for skeletal development. |
Chondroitin sulfate synthase-2/chondroitin polymerizing factor has two variants with distinct function. |
