Smg8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smg8
Entrez Gene ID	74133
Full Name	smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans)
Synonyms	1200011M11Rik
General protein information	Preferred Names smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans) Names protein SMG8 CG6729 gene product protein smg-8 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_024262.1	NP_077224.1	protein SMG8

Pathways from BioSystems

Homologies

Homo sapiens (human)	SMG8	NP_060619.4
Xenopus tropicalis (tropical clawed frog)	smg8	NP_001072291.1
Bos taurus (cattle)	SMG8	NP_001073755.1
Mus musculus (house mouse)	Smg8	NP_077224.1
	C17orf71	XP_001107695.1
Canis lupus familiaris (dog)	SMG8	XP_548236.1
Rattus norvegicus (Norway rat)	Smg8	XP_213430.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010179	XP_319357.4
Pan troglodytes (chimpanzee)	SMG8	XP_523682.1
Gallus gallus (chicken)	SMG8	XP_415875.4
Danio rerio (zebrafish)	smg8	XP_695811.2
Drosophila melanogaster (fruit fly)	CG6729	NP_609449.1

Gene Ontology
Bibliography

Related articles in PubMed

A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome.
Hansen J, Floss T, Van Sloun P, F?chtbauer EM, Vauti F, Arnold HH, Schn?tgen F, Wurst W, von Melchner H, Ruiz P
Proceedings of the National Academy of Sciences of the United States of America100(17)9918-22(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Smg8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14554	NM_024262.1 Latest version!	Mus musculus smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$580.30 $829.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smg8 ( NM_024262.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077224.1 Mus musculus smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg8), mRNA.

Price & Availability↑

CloneID	OMu14554
Clone ID Related Accession (Same CDS sequence)	NM_024262.1
Accession Version	NM_024262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Mus musculus(house mouse)
Product	protein SMG8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC020005.1. On Dec 7, 2005 this sequence version replaced XM_914416.1. ##Evidence-Data-START## Transcript exon combination :: BC020005.1, AK004714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164133 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGGC CGGTCAGCTT GCGCGAGCTG CTCATGGGGG CGTCGGCCTG GCTGGGCTCT 
GAGAGTCCTG GAGGCCCCTC CGCTGAAGGC GGGGGAAACG CGGCTGGCAC GCCCGAGCCC 
CCGTGGAGGG AGGATGAGAT CTGCGTGGTG GGAATCTTCG GCAAGACGGC TCTGCGCCTC 
AACTCCGAGA AGTTCTCTCT GGTGAATACT GTGTGCGATC GACAGGTTTT CCCACTCTTT 
CACCATCAGG ATCCTGGAGA TCCAGGAACT GGAATCAAGA CCAAAGCTGT CTCTGTCGGA 
GAGGCGGGAG GAGCCGGAGA CCCCGGGGCT GCAGCCGGGG ATTCACTTCG CGGAGGTATG 
GCTACGGCGG AAGGTAACCG AGCTGAGCCA GGTCCCCAGG ACTTCAGTCT TTTGCAGGCC 
TATTACAATC AGGAAAGCAA AGTTCTGTAT CTTATTCTCA CTTCCATCTG TGACAACTCA 
CAGCTTCTGC GTGCTTGTCG GGCCCTCCAG AGCGGTGAAG CCGGAGGGGG CCTCTCTTTA 
CCTCATGCAG AAACACACGA GTTCTGGAAG CATCAGGAGA AGTTACAGTG TCTCAGCCTC 
CTTTACCTCT TCTCTGTCTG TCATATCTTG CTCCTGGTCC ATCCGACGTG TTCCTTTGAC 
ATCACTTATG ATCGTGTATT CAGAGCCCTG GATGGACTGA GACAGAAAGT ACTACCCCTC 
CTCAAAACAG CCATTAAGGA TTGCCCAGTT GGCAAAGACT GGAAGTTAAA CTGCCGACCT 
TGCCCACCTA GACTCCTCTT CCTCTTTCAA CTCAATGGGG CCCTTAAGGT GGAACCGCCT 
CGGAGCCAGG ATACAGCTCA TCCAGATAAA CCCAAGAAGC ACTCTCCCAA AAGAAGGCTG 
CAGCATGCCC TGGAGGACCA GATCTATAGA ATCTTTCGCA AGAGTCGAGT CTTGACTAAT 
CAGAGTATCA ACTGCCTCTT TACGGTACCT GCCAACCAAG CTTTTGTGTA CATAGTCCCC 
GGAAGCCAGG AGGAGGACCC AATTGGCATG TTGCTGGACC AGCTTAGGAG CCATTGTACC 
GTGAAAGACC CAGAATCGTT GTTGGTGCCC GCACCCCTTT CTGGACCTAG GCGGTACCAA 
GCGATGAGAC AGCACAGCCG CCAACAGCTC TCCTTCCACA TTGATAGCAG CACCTCGAGT 
TCTTCAGGCC AGCTAGTGGA TTTCACTCTT CGAGAATTCC TGTGGCAGCA CGTGGAGCTG 
GTCCTAAGCA AAAAAGGTTT TGATGACAGT GTGGGCAGGA ACCCACAGCC TTCCCATTTT 
GAACTGCCCA CTTACCAGAA GTGGATCTCT GCAGCAGCAA AGTTGTATGA AGTGGCTATC 
GATGGGAAGG AAGAGGACTT GGGCTCCCCC ACTGGGGAGC TAACATCTAA GATTTTAAGC 
AGTATTAAAG TCTTAGAAGG GTTTTTGGAT ATAGACACAA AATTCTCGGA AAACCGGTGC 
CAAAAAGCTT TACCCATGGC TCATAGTGCC TACCAGTCAA ACTTGCCGCA TAATTACACC 
ATGACAGTCC ATAAGAATCA GCTTGCTCAG GCTCTTCGGG TATACAGTCA GCATGCTCGA 
GGTCCAGCCT TTCACAAATA TGCCATGCAA TTACACGAAG ACTGCTACAA ATTTTGGAGC 
AATGGCCATC AGCTGTGCGA AGAGAGGAGC TTGACTGACC AACACTGTGT CCACAAATTT 
CATTCCTTAC CTAAATCAGG AGAAAAGCCA GAGGCAGATA GGAACCCTCC TGTGCTCTAC 
CACAATAGCC GAGCCCGATC CACCGGAGCC TGCAACTGCG GAAGGAAGCA GGCACCTCGA 
GACGACCCCT TCGATATCAA GGCTGCTAAC TATGACTTTT ACCAGCTTCT GGAAGAAAAA 
TGTTGTGGGA AATTGGATCA TATCAATTTC CCTGTATTTG AGCCAAGTAC TCCAGATCCC 
GCTCCTGCTA AAAATGAACC CTCTCCTGCC CCGCCAGACT CGGACGCCGA GAAACTGAAG 
GAGAAAGAAC CTCAAACCCA AGGAGAGAGC ACAAGCCTCA GCTTAGCTTT GAGCTTGGGC 
CAGTCAACAG ATAGCCTAGG TACCTACCCC GCTGATCCAC AGGCAGGAGG AGACAACCCA 
GAAGTCCACG GGCAAGGAGA AGGAAAGTCT GAGAAGAGAC CAAACTTGGT TGACCGTCAG 
GCATCCACAG TTGAATATCT CCCAGGCATG CTACATTCAA ATTGCCCCAA AGGTCTCCTA 
CCCAAATTCT CCAGCTGGTC TTTGGTTAAA CTAGGCCCAG CCAAGTCTTA CAACTTTCAT 
ACAGGTTTGG ACCAACAGGG CTTCGTTCCA GGAACAAACT ATCTTATGCC TTGGGACATT 
GTCATCAGGA CTAGAGCTGA AGATGAAGGA GACTTAGACA CGAACTCCTG GCCTGCTCCA 
AATAAAGCTA TTCCTGGGAA GAGAAGTGCA GTTGTGATGG GCAGAGGAAG GCGGAGGGAT 
GACATAGCTC GAGCCTTTGT TGGCTTTGAG TATGAAGACT CTCGAGGTCG GAGGTTCATG 
TGCTCAGGGC CTGACAAAGT AATGAAAGTG ATGGGAAGTG GACCCAAGGA GTCGGCTTTA 
AAAGCCCTCA ACAGTGACAT GCCCCTGTAC ATCCTGTCCT CGTCGCAGGG CAGAGGGCTG 
AAGCCACATT ATGCTCAACT TATGAGGCTT TTTGTTGTGG TTCCTGATGC TCCTTTGCAG 
ATAATACTGA TGCCTCAGGT TCAACCAGGC CCACCACCAT GTCCAGTATT TTACCCAGAA 
AAACAAGAAA TCACTCTCCC ACCTGATGGC CTTTGGGTCT TGAGATTTCC TTATGCCTAT 
GTGACTGAGA GAGGACCTTG TTTCCCTCCC AAGGAGAATG TGCAGCTCAT GAGCTACAAG 
GTGCTCCGAG GAGTCCTCAA AGCAGTTACA CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077224.1
CDS	25..3000
Translation	MAGPVSLRELLMGASAWLGSESPGGPSAEGGGNAAGTPEPPWREDEICVVGIFGKTALRLNSEKFSLVNTVCDRQVFPLFHHQDPGDPGTGIKTKAVSVGEAGGAGDPGAAAGDSLRGGMATAEGNRAEPGPQDFSLLQAYYNQESKVLYLILTSICDNSQLLRACRALQSGEAGGGLSLPHAETHEFWKHQEKLQCLSLLYLFSVCHILLLVHPTCSFDITYDRVFRALDGLRQKVLPLLKTAIKDCPVGKDWKLNCRPCPPRLLFLFQLNGALKVEPPRSQDTAHPDKPKKHSPKRRLQHALEDQIYRIFRKSRVLTNQSINCLFTVPANQAFVYIVPGSQEEDPIGMLLDQLRSHCTVKDPESLLVPAPLSGPRRYQAMRQHSRQQLSFHIDSSTSSSSGQLVDFTLREFLWQHVELVLSKKGFDDSVGRNPQPSHFELPTYQKWISAAAKLYEVAIDGKEEDLGSPTGELTSKILSSIKVLEGFLDIDTKFSENRCQKALPMAHSAYQSNLPHNYTMTVHKNQLAQALRVYSQHARGPAFHKYAMQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHSLPKSGEKPEADRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAANYDFYQLLEEKCCGKLDHINFPVFEPSTPDPAPAKNEPSPAPPDSDAEKLKEKEPQTQGESTSLSLALSLGQSTDSLGTYPADPQAGGDNPEVHGQGEGKSEKRPNLVDRQASTVEYLPGMLHSNCPKGLLPKFSSWSLVKLGPAKSYNFHTGLDQQGFVPGTNYLMPWDIVIRTRAEDEGDLDTNSWPAPNKAIPGKRSAVVMGRGRRRDDIARAFVGFEYEDSRGRRFMCSGPDKVMKVMGSGPKESALKALNSDMPLYILSSSQGRGLKPHYAQLMRLFVVVPDAPLQIILMPQVQPGPPPCPVFYPEKQEITLPPDGLWVLRFPYAYVTERGPCFPPKENVQLMSYKVLRGVLKAVTQ

Target ORF information:

RefSeq Version	NM_024262.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024262.1

ORF Insert Sequence:

ATGGCGGGGC CGGTCAGCTT GCGCGAGCTG CTCATGGGGG CGTCGGCCTG GCTGGGCTCT 
GAGAGTCCTG GAGGCCCCTC CGCTGAAGGC GGGGGAAACG CGGCTGGCAC GCCCGAGCCC 
CCGTGGAGGG AGGATGAGAT CTGCGTGGTG GGAATCTTCG GCAAGACGGC TCTGCGCCTC 
AACTCCGAGA AGTTCTCTCT GGTGAATACT GTGTGCGATC GACAGGTTTT CCCACTCTTT 
CACCATCAGG ATCCTGGAGA TCCAGGAACT GGAATCAAGA CCAAAGCTGT CTCTGTCGGA 
GAGGCGGGAG GAGCCGGAGA CCCCGGGGCT GCAGCCGGGG ATTCACTTCG CGGAGGTATG 
GCTACGGCGG AAGGTAACCG AGCTGAGCCA GGTCCCCAGG ACTTCAGTCT TTTGCAGGCC 
TATTACAATC AGGAAAGCAA AGTTCTGTAT CTTATTCTCA CTTCCATCTG TGACAACTCA 
CAGCTTCTGC GTGCTTGTCG GGCCCTCCAG AGCGGTGAAG CCGGAGGGGG CCTCTCTTTA 
CCTCATGCAG AAACACACGA GTTCTGGAAG CATCAGGAGA AGTTACAGTG TCTCAGCCTC 
CTTTACCTCT TCTCTGTCTG TCATATCTTG CTCCTGGTCC ATCCGACGTG TTCCTTTGAC 
ATCACTTATG ATCGTGTATT CAGAGCCCTG GATGGACTGA GACAGAAAGT ACTACCCCTC 
CTCAAAACAG CCATTAAGGA TTGCCCAGTT GGCAAAGACT GGAAGTTAAA CTGCCGACCT 
TGCCCACCTA GACTCCTCTT CCTCTTTCAA CTCAATGGGG CCCTTAAGGT GGAACCGCCT 
CGGAGCCAGG ATACAGCTCA TCCAGATAAA CCCAAGAAGC ACTCTCCCAA AAGAAGGCTG 
CAGCATGCCC TGGAGGACCA GATCTATAGA ATCTTTCGCA AGAGTCGAGT CTTGACTAAT 
CAGAGTATCA ACTGCCTCTT TACGGTACCT GCCAACCAAG CTTTTGTGTA CATAGTCCCC 
GGAAGCCAGG AGGAGGACCC AATTGGCATG TTGCTGGACC AGCTTAGGAG CCATTGTACC 
GTGAAAGACC CAGAATCGTT GTTGGTGCCC GCACCCCTTT CTGGACCTAG GCGGTACCAA 
GCGATGAGAC AGCACAGCCG CCAACAGCTC TCCTTCCACA TTGATAGCAG CACCTCGAGT 
TCTTCAGGCC AGCTAGTGGA TTTCACTCTT CGAGAATTCC TGTGGCAGCA CGTGGAGCTG 
GTCCTAAGCA AAAAAGGTTT TGATGACAGT GTGGGCAGGA ACCCACAGCC TTCCCATTTT 
GAACTGCCCA CTTACCAGAA GTGGATCTCT GCAGCAGCAA AGTTGTATGA AGTGGCTATC 
GATGGGAAGG AAGAGGACTT GGGCTCCCCC ACTGGGGAGC TAACATCTAA GATTTTAAGC 
AGTATTAAAG TCTTAGAAGG GTTTTTGGAT ATAGACACAA AATTCTCGGA AAACCGGTGC 
CAAAAAGCTT TACCCATGGC TCATAGTGCC TACCAGTCAA ACTTGCCGCA TAATTACACC 
ATGACAGTCC ATAAGAATCA GCTTGCTCAG GCTCTTCGGG TATACAGTCA GCATGCTCGA 
GGTCCAGCCT TTCACAAATA TGCCATGCAA TTACACGAAG ACTGCTACAA ATTTTGGAGC 
AATGGCCATC AGCTGTGCGA AGAGAGGAGC TTGACTGACC AACACTGTGT CCACAAATTT 
CATTCCTTAC CTAAATCAGG AGAAAAGCCA GAGGCAGATA GGAACCCTCC TGTGCTCTAC 
CACAATAGCC GAGCCCGATC CACCGGAGCC TGCAACTGCG GAAGGAAGCA GGCACCTCGA 
GACGACCCCT TCGATATCAA GGCTGCTAAC TATGACTTTT ACCAGCTTCT GGAAGAAAAA 
TGTTGTGGGA AATTGGATCA TATCAATTTC CCTGTATTTG AGCCAAGTAC TCCAGATCCC 
GCTCCTGCTA AAAATGAACC CTCTCCTGCC CCGCCAGACT CGGACGCCGA GAAACTGAAG 
GAGAAAGAAC CTCAAACCCA AGGAGAGAGC ACAAGCCTCA GCTTAGCTTT GAGCTTGGGC 
CAGTCAACAG ATAGCCTAGG TACCTACCCC GCTGATCCAC AGGCAGGAGG AGACAACCCA 
GAAGTCCACG GGCAAGGAGA AGGAAAGTCT GAGAAGAGAC CAAACTTGGT TGACCGTCAG 
GCATCCACAG TTGAATATCT CCCAGGCATG CTACATTCAA ATTGCCCCAA AGGTCTCCTA 
CCCAAATTCT CCAGCTGGTC TTTGGTTAAA CTAGGCCCAG CCAAGTCTTA CAACTTTCAT 
ACAGGTTTGG ACCAACAGGG CTTCGTTCCA GGAACAAACT ATCTTATGCC TTGGGACATT 
GTCATCAGGA CTAGAGCTGA AGATGAAGGA GACTTAGACA CGAACTCCTG GCCTGCTCCA 
AATAAAGCTA TTCCTGGGAA GAGAAGTGCA GTTGTGATGG GCAGAGGAAG GCGGAGGGAT 
GACATAGCTC GAGCCTTTGT TGGCTTTGAG TATGAAGACT CTCGAGGTCG GAGGTTCATG 
TGCTCAGGGC CTGACAAAGT AATGAAAGTG ATGGGAAGTG GACCCAAGGA GTCGGCTTTA 
AAAGCCCTCA ACAGTGACAT GCCCCTGTAC ATCCTGTCCT CGTCGCAGGG CAGAGGGCTG 
AAGCCACATT ATGCTCAACT TATGAGGCTT TTTGTTGTGG TTCCTGATGC TCCTTTGCAG 
ATAATACTGA TGCCTCAGGT TCAACCAGGC CCACCACCAT GTCCAGTATT TTACCCAGAA 
AAACAAGAAA TCACTCTCCC ACCTGATGGC CTTTGGGTCT TGAGATTTCC TTATGCCTAT 
GTGACTGAGA GAGGACCTTG TTTCCCTCCC AAGGAGAATG TGCAGCTCAT GAGCTACAAG 
GTGCTCCGAG GAGTCCTCAA AGCAGTTACA CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome.
Proceedings of the National Academy of Sciences of the United States of America100(17)9918-22(2003 Aug)
Hansen J,Floss T,Van Sloun P,F?chtbauer EM,Vauti F,Arnold HH,Schn?tgen F,Wurst W,von Melchner H,Ruiz P

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Smg8 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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