Rnf6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf6
Entrez Gene ID	74132
Full Name	ring finger protein (C3H2C3 type) 6
Synonyms	1200013I08Rik,5730419H05Rik,AA537053
General protein information	Preferred Names ring finger protein (C3H2C3 type) 6 Names E3 ubiquitin-protein ligase RNF6 RING-type E3 ubiquitin transferase RNF6 RLIM-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256086.1	NP_001243015.1	E3 ubiquitin-protein ligase RNF6 isoform 2
NM_001256085.1	NP_001243014.1	E3 ubiquitin-protein ligase RNF6 isoform 1
NM_001256087.1	NP_001243016.1	E3 ubiquitin-protein ligase RNF6 isoform 1
NM_028774.3	NP_083050.1	E3 ubiquitin-protein ligase RNF6 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF6	NP_005968.1
Gallus gallus (chicken)	RNF6	XP_004938841.1
Pan troglodytes (chimpanzee)	RNF6	XP_001156347.1
Canis lupus familiaris (dog)	RNF6	XP_005635591.1
Mus musculus (house mouse)	Rnf6	NP_001243015.1
Rattus norvegicus (Norway rat)	Rnf6	NP_001100588.1
Bos taurus (cattle)	RNF6	NP_001091531.1
	RNF6	XP_002800736.1

Gene Ontology
Bibliography

Related articles in PubMed

Islet ?-, ?-, and ?-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor.
Hunter CS, Dixit S, Cohen T, Ediger B, Wilcox C, Ferreira M, Westphal H, Stein R, May CL
Diabetes62(3)875-86(2013 Mar)

Distinct gene loci control the host response to influenza H1N1 virus infection in a time-dependent manner.
Nedelko T, Kollmus H, Klawonn F, Spijker S, Lu L, He?man M, Alberts R, Williams RW, Schughart K
BMC genomics13411(2012 Aug)

The ubiquitin ligase Rnf6 regulates local LIM kinase 1 levels in axonal growth cones.
Tursun B, Schl?ter A, Peters MA, Viehweger B, Ostendorff HP, Soosairajah J, Drung A, Bossenz M, Johnsen SA, Schweizer M, Bernard O, Bach I
Genes & development19(19)2307-19(2005 Oct)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Gene control in germinal differentiation: RNF6, a transcription regulatory protein in the mouse sertoli cell.
Lopez P, Vidal F, Martin L, Lopez-Fernandez LA, Rual JF, Rosen BS, Cuzin F, Rassoulzadegan M
Molecular and cellular biology22(10)3488-96(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rnf6 controls cellular LIMK1 concentrations and indicate a new function for the ubiquitin/proteasome system in regulating local growth cone actin dynamics
Title: The ubiquitin ligase Rnf6 regulates local LIM kinase 1 levels in axonal growth cones.

The following Rnf6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05990	NM_001256085.1 Latest version!	Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu05990	NM_001256087.1 Latest version!	Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu05990	NM_028774.3 Latest version!	Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu05991	NM_001256086.1 Latest version!	Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf6 ( NM_001256085.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243014.1 Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05990
Clone ID Related Accession (Same CDS sequence)	NM_001256085.1 , NM_001256087.1 , NM_028774.3
Accession Version	NM_001256085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY136428.1, AK150269.1, AK004745.1 and AV586321.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK150269.1, AK152106.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATCCGT CTAGATCTAG ATCAGGTGGG AGTGGAGAAG AGAGCTCATT TCAAGAAAAT 
GAGAGAAGAT GGCAGCAAGA ACGTCTCCAC AGAGAAGAAG CCTATTATCA GTTTATTAAT 
GAGCTCAGTG ATGAAGATTA TCGGCTAATG AGAGACCATA ATCTTTTAGG CACCCCTGGA 
GAAATAACAT CAGAGGAGCT GCAGCAGCGC TTAGAGAGAG CAAAGGAACA GCTGGCATCT 
CAGCCTGGCT CTGACAGCGC GGCAAGTGAT GGAGACTCAG AAAGCCTTAG AGCACATTCA 
GATGAGGACT CTCTGCTGCG ATGGCTGAAT ACCTTTCGGC GCACTGGGAA TGTGACTCGA 
AGCGGACAGA ATGGGAACCA AAGCTGGAGG GCCGTGAGTC GAACAAACCC GAACAGTGGC 
GAGTTCGGGT TTAGCCTGGA AATCCACATA AACCCTGACA ACAGAGGTTC TGAAATGCAT 
GGAGAGGACT CGACGGACAT CCCTCTGTCA GGTGTTAACA GAGAGCATAG GCAGCAAAGA 
CCATCGAGTC CTGTGGCTAG GCGAACCAGA AGCCAGACCT CCATGAGCTC CAGTGGTCCT 
AGGGGCAGGC GTGGTGCGAG GAGGCAGGGT TCTGTGCAAG GGTCATTTGC AACTCTGGGA 
AGGTTAAGGA ATGGAATTGG AGTAGCCCTT GGTGTTCCCA GAGTTAGTGC CCCTCGCACT 
AATGTTATTA ATTCCCACAC CAACCAGTCA GATGGTAGTA CTCTCAGGCA AGGTGGACGG 
CAGCGGTTTG GAGCAGCACA TATTTGGGAA AACGGGGCTA GAAGTAATGT TACAGTGAGG 
AACACAAACC AAAGATTAGA GCCAATAAGA TTACGTCCTG CTTTCAGTAG TCGAAGCCGG 
TCCCCCATTC AGAGACAGAA TGGTACTGTT CATCATAATT CCCAAAGGCA GGGTAGACCA 
GTACAGCAAA CGGGTAGAAA CAGGTCTGTT AGGCACAGAG GTGTAACGCG GGTCTTTTTA 
GAACAAGGTA GAGAACACAG AGGCACTGAT TACACCCCGC TGTCCAACTC AAGACTTGTG 
TCAAGAATAA CAGTAGAAGA GGGAGAGTCT AGCAGGTCCT CAGCTGCCAC CCAGCGTCAT 
CCAGCAATCA CACTGGACCT GCAAGTCAGG AGGATTCGGC CTGGAGAAAC TAGAGATAGG 
GATAGCATTG CAAATAGAAC CCGATCTAGG GCGGGGCTGG CAGAAAGTAC GGTTGAGAGC 
ACCAGTGGGG GCTTCCACAG AACTATCTCT CACTTAGAGC GCTCAGGTGT CCGAACCTAC 
GTGAGCACCA TCACAGTTCC TCTTCGCAGG ATTTCTGAGA ATGACGTGGT CGAGCCATCC 
TCCGTGGCTC TTCGGTCAAT TTTAAGGCAG ATCATGACTG GGTTTGGAGA GCTGAGTTCT 
CTAATGGAGG TGGAGCCTGC GTCAGAGAAT CAAAGCAATG GCCAACGTTT ACCAGAGGTG 
TACCTAGAAC TGAGTAATGG AGATGCAGCC GATGACAGTG GTCAGCATGG AAGAGCGTCC 
TCACAGGCCA GTCAGGCCCA GGACGGTGCT GAAATGCTAG CTGTGAGGGA GCCCGCCCCG 
CCCCAGGCTC GCCCCAGTGG CAGTAGAAGT AGAAGGCAGT TAGGAAGGGC GGACAGCGTA 
GTTGAGGCTG GAACATTACC CATTCTGCGC CTTGCTCACT TCTTCTTACT GAATGAAGGC 
GATGATGATC CAATTCGTGG TTTAACCAAA GAGCAGATTG ACAATCTTTC TACCAGGAGT 
TATGAACAAG ACGGGGTTGA CAGTGAGTTA GGTAAGGTGT GTAGCGTGTG CATCAGTGAC 
TACGTGGCTG GGAACAAGCT CCGGCAGCTG CCTTGTCTGC ACGAGTTTCA CATTCACTGT 
ATTGATCGCT GGCTGTCGGA GAACTGCACC TGCCCCGTCT GTCGGCGGCC TGTCTTAGAA 
TTCGGAGCCA CAAGCAGTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243014.1
CDS	134..2137
Translation	MDPSRSRSGGSGEESSFQENERRWQQERLHREEAYYQFINELSDEDYRLMRDHNLLGTPGEITSEELQQRLERAKEQLASQPGSDSAASDGDSESLRAHSDEDSLLRWLNTFRRTGNVTRSGQNGNQSWRAVSRTNPNSGEFGFSLEIHINPDNRGSEMHGEDSTDIPLSGVNREHRQQRPSSPVARRTRSQTSMSSSGPRGRRGARRQGSVQGSFATLGRLRNGIGVALGVPRVSAPRTNVINSHTNQSDGSTLRQGGRQRFGAAHIWENGARSNVTVRNTNQRLEPIRLRPAFSSRSRSPIQRQNGTVHHNSQRQGRPVQQTGRNRSVRHRGVTRVFLEQGREHRGTDYTPLSNSRLVSRITVEEGESSRSSAATQRHPAITLDLQVRRIRPGETRDRDSIANRTRSRAGLAESTVESTSGGFHRTISHLERSGVRTYVSTITVPLRRISENDVVEPSSVALRSILRQIMTGFGELSSLMEVEPASENQSNGQRLPEVYLELSNGDAADDSGQHGRASSQASQAQDGAEMLAVREPAPPQARPSGSRSRRQLGRADSVVEAGTLPILRLAHFFLLNEGDDDPIRGLTKEQIDNLSTRSYEQDGVDSELGKVCSVCISDYVAGNKLRQLPCLHEFHIHCIDRWLSENCTCPVCRRPVLEFGATSSG

Target ORF information:

RefSeq Version	NM_001256085.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256085.1

ORF Insert Sequence:

ATGGATCCGT CTAGATCTAG ATCAGGTGGG AGTGGAGAAG AGAGCTCATT TCAAGAAAAT 
GAGAGAAGAT GGCAGCAAGA ACGTCTCCAC AGAGAAGAAG CCTATTATCA GTTTATTAAT 
GAGCTCAGTG ATGAAGATTA TCGGCTAATG AGAGACCATA ATCTTTTAGG CACCCCTGGA 
GAAATAACAT CAGAGGAGCT GCAGCAGCGC TTAGAGAGAG CAAAGGAACA GCTGGCATCT 
CAGCCTGGCT CTGACAGCGC GGCAAGTGAT GGAGACTCAG AAAGCCTTAG AGCACATTCA 
GATGAGGACT CTCTGCTGCG ATGGCTGAAT ACCTTTCGGC GCACTGGGAA TGTGACTCGA 
AGCGGACAGA ATGGGAACCA AAGCTGGAGG GCCGTGAGTC GAACAAACCC GAACAGTGGC 
GAGTTCGGGT TTAGCCTGGA AATCCACATA AACCCTGACA ACAGAGGTTC TGAAATGCAT 
GGAGAGGACT CGACGGACAT CCCTCTGTCA GGTGTTAACA GAGAGCATAG GCAGCAAAGA 
CCATCGAGTC CTGTGGCTAG GCGAACCAGA AGCCAGACCT CCATGAGCTC CAGTGGTCCT 
AGGGGCAGGC GTGGTGCGAG GAGGCAGGGT TCTGTGCAAG GGTCATTTGC AACTCTGGGA 
AGGTTAAGGA ATGGAATTGG AGTAGCCCTT GGTGTTCCCA GAGTTAGTGC CCCTCGCACT 
AATGTTATTA ATTCCCACAC CAACCAGTCA GATGGTAGTA CTCTCAGGCA AGGTGGACGG 
CAGCGGTTTG GAGCAGCACA TATTTGGGAA AACGGGGCTA GAAGTAATGT TACAGTGAGG 
AACACAAACC AAAGATTAGA GCCAATAAGA TTACGTCCTG CTTTCAGTAG TCGAAGCCGG 
TCCCCCATTC AGAGACAGAA TGGTACTGTT CATCATAATT CCCAAAGGCA GGGTAGACCA 
GTACAGCAAA CGGGTAGAAA CAGGTCTGTT AGGCACAGAG GTGTAACGCG GGTCTTTTTA 
GAACAAGGTA GAGAACACAG AGGCACTGAT TACACCCCGC TGTCCAACTC AAGACTTGTG 
TCAAGAATAA CAGTAGAAGA GGGAGAGTCT AGCAGGTCCT CAGCTGCCAC CCAGCGTCAT 
CCAGCAATCA CACTGGACCT GCAAGTCAGG AGGATTCGGC CTGGAGAAAC TAGAGATAGG 
GATAGCATTG CAAATAGAAC CCGATCTAGG GCGGGGCTGG CAGAAAGTAC GGTTGAGAGC 
ACCAGTGGGG GCTTCCACAG AACTATCTCT CACTTAGAGC GCTCAGGTGT CCGAACCTAC 
GTGAGCACCA TCACAGTTCC TCTTCGCAGG ATTTCTGAGA ATGACGTGGT CGAGCCATCC 
TCCGTGGCTC TTCGGTCAAT TTTAAGGCAG ATCATGACTG GGTTTGGAGA GCTGAGTTCT 
CTAATGGAGG TGGAGCCTGC GTCAGAGAAT CAAAGCAATG GCCAACGTTT ACCAGAGGTG 
TACCTAGAAC TGAGTAATGG AGATGCAGCC GATGACAGTG GTCAGCATGG AAGAGCGTCC 
TCACAGGCCA GTCAGGCCCA GGACGGTGCT GAAATGCTAG CTGTGAGGGA GCCCGCCCCG 
CCCCAGGCTC GCCCCAGTGG CAGTAGAAGT AGAAGGCAGT TAGGAAGGGC GGACAGCGTA 
GTTGAGGCTG GAACATTACC CATTCTGCGC CTTGCTCACT TCTTCTTACT GAATGAAGGC 
GATGATGATC CAATTCGTGG TTTAACCAAA GAGCAGATTG ACAATCTTTC TACCAGGAGT 
TATGAACAAG ACGGGGTTGA CAGTGAGTTA GGTAAGGTGT GTAGCGTGTG CATCAGTGAC 
TACGTGGCTG GGAACAAGCT CCGGCAGCTG CCTTGTCTGC ACGAGTTTCA CATTCACTGT 
ATTGATCGCT GGCTGTCGGA GAACTGCACC TGCCCCGTCT GTCGGCGGCC TGTCTTAGAA 
TTCGGAGCCA CAAGCAGTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf6 ( NM_001256087.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243016.1 Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu05990
Clone ID Related Accession (Same CDS sequence)	NM_001256085.1 , NM_001256087.1 , NM_028774.3
Accession Version	NM_001256087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX237430.1, AK004745.1 and AV586321.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: CX237430.1, SRR1660811.358896.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGATCCGT CTAGATCTAG ATCAGGTGGG AGTGGAGAAG AGAGCTCATT TCAAGAAAAT 
GAGAGAAGAT GGCAGCAAGA ACGTCTCCAC AGAGAAGAAG CCTATTATCA GTTTATTAAT 
GAGCTCAGTG ATGAAGATTA TCGGCTAATG AGAGACCATA ATCTTTTAGG CACCCCTGGA 
GAAATAACAT CAGAGGAGCT GCAGCAGCGC TTAGAGAGAG CAAAGGAACA GCTGGCATCT 
CAGCCTGGCT CTGACAGCGC GGCAAGTGAT GGAGACTCAG AAAGCCTTAG AGCACATTCA 
GATGAGGACT CTCTGCTGCG ATGGCTGAAT ACCTTTCGGC GCACTGGGAA TGTGACTCGA 
AGCGGACAGA ATGGGAACCA AAGCTGGAGG GCCGTGAGTC GAACAAACCC GAACAGTGGC 
GAGTTCGGGT TTAGCCTGGA AATCCACATA AACCCTGACA ACAGAGGTTC TGAAATGCAT 
GGAGAGGACT CGACGGACAT CCCTCTGTCA GGTGTTAACA GAGAGCATAG GCAGCAAAGA 
CCATCGAGTC CTGTGGCTAG GCGAACCAGA AGCCAGACCT CCATGAGCTC CAGTGGTCCT 
AGGGGCAGGC GTGGTGCGAG GAGGCAGGGT TCTGTGCAAG GGTCATTTGC AACTCTGGGA 
AGGTTAAGGA ATGGAATTGG AGTAGCCCTT GGTGTTCCCA GAGTTAGTGC CCCTCGCACT 
AATGTTATTA ATTCCCACAC CAACCAGTCA GATGGTAGTA CTCTCAGGCA AGGTGGACGG 
CAGCGGTTTG GAGCAGCACA TATTTGGGAA AACGGGGCTA GAAGTAATGT TACAGTGAGG 
AACACAAACC AAAGATTAGA GCCAATAAGA TTACGTCCTG CTTTCAGTAG TCGAAGCCGG 
TCCCCCATTC AGAGACAGAA TGGTACTGTT CATCATAATT CCCAAAGGCA GGGTAGACCA 
GTACAGCAAA CGGGTAGAAA CAGGTCTGTT AGGCACAGAG GTGTAACGCG GGTCTTTTTA 
GAACAAGGTA GAGAACACAG AGGCACTGAT TACACCCCGC TGTCCAACTC AAGACTTGTG 
TCAAGAATAA CAGTAGAAGA GGGAGAGTCT AGCAGGTCCT CAGCTGCCAC CCAGCGTCAT 
CCAGCAATCA CACTGGACCT GCAAGTCAGG AGGATTCGGC CTGGAGAAAC TAGAGATAGG 
GATAGCATTG CAAATAGAAC CCGATCTAGG GCGGGGCTGG CAGAAAGTAC GGTTGAGAGC 
ACCAGTGGGG GCTTCCACAG AACTATCTCT CACTTAGAGC GCTCAGGTGT CCGAACCTAC 
GTGAGCACCA TCACAGTTCC TCTTCGCAGG ATTTCTGAGA ATGACGTGGT CGAGCCATCC 
TCCGTGGCTC TTCGGTCAAT TTTAAGGCAG ATCATGACTG GGTTTGGAGA GCTGAGTTCT 
CTAATGGAGG TGGAGCCTGC GTCAGAGAAT CAAAGCAATG GCCAACGTTT ACCAGAGGTG 
TACCTAGAAC TGAGTAATGG AGATGCAGCC GATGACAGTG GTCAGCATGG AAGAGCGTCC 
TCACAGGCCA GTCAGGCCCA GGACGGTGCT GAAATGCTAG CTGTGAGGGA GCCCGCCCCG 
CCCCAGGCTC GCCCCAGTGG CAGTAGAAGT AGAAGGCAGT TAGGAAGGGC GGACAGCGTA 
GTTGAGGCTG GAACATTACC CATTCTGCGC CTTGCTCACT TCTTCTTACT GAATGAAGGC 
GATGATGATC CAATTCGTGG TTTAACCAAA GAGCAGATTG ACAATCTTTC TACCAGGAGT 
TATGAACAAG ACGGGGTTGA CAGTGAGTTA GGTAAGGTGT GTAGCGTGTG CATCAGTGAC 
TACGTGGCTG GGAACAAGCT CCGGCAGCTG CCTTGTCTGC ACGAGTTTCA CATTCACTGT 
ATTGATCGCT GGCTGTCGGA GAACTGCACC TGCCCCGTCT GTCGGCGGCC TGTCTTAGAA 
TTCGGAGCCA CAAGCAGTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243016.1
CDS	266..2269
Translation	MDPSRSRSGGSGEESSFQENERRWQQERLHREEAYYQFINELSDEDYRLMRDHNLLGTPGEITSEELQQRLERAKEQLASQPGSDSAASDGDSESLRAHSDEDSLLRWLNTFRRTGNVTRSGQNGNQSWRAVSRTNPNSGEFGFSLEIHINPDNRGSEMHGEDSTDIPLSGVNREHRQQRPSSPVARRTRSQTSMSSSGPRGRRGARRQGSVQGSFATLGRLRNGIGVALGVPRVSAPRTNVINSHTNQSDGSTLRQGGRQRFGAAHIWENGARSNVTVRNTNQRLEPIRLRPAFSSRSRSPIQRQNGTVHHNSQRQGRPVQQTGRNRSVRHRGVTRVFLEQGREHRGTDYTPLSNSRLVSRITVEEGESSRSSAATQRHPAITLDLQVRRIRPGETRDRDSIANRTRSRAGLAESTVESTSGGFHRTISHLERSGVRTYVSTITVPLRRISENDVVEPSSVALRSILRQIMTGFGELSSLMEVEPASENQSNGQRLPEVYLELSNGDAADDSGQHGRASSQASQAQDGAEMLAVREPAPPQARPSGSRSRRQLGRADSVVEAGTLPILRLAHFFLLNEGDDDPIRGLTKEQIDNLSTRSYEQDGVDSELGKVCSVCISDYVAGNKLRQLPCLHEFHIHCIDRWLSENCTCPVCRRPVLEFGATSSG

Target ORF information:

RefSeq Version	NM_001256087.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256087.1

ORF Insert Sequence:

ATGGATCCGT CTAGATCTAG ATCAGGTGGG AGTGGAGAAG AGAGCTCATT TCAAGAAAAT 
GAGAGAAGAT GGCAGCAAGA ACGTCTCCAC AGAGAAGAAG CCTATTATCA GTTTATTAAT 
GAGCTCAGTG ATGAAGATTA TCGGCTAATG AGAGACCATA ATCTTTTAGG CACCCCTGGA 
GAAATAACAT CAGAGGAGCT GCAGCAGCGC TTAGAGAGAG CAAAGGAACA GCTGGCATCT 
CAGCCTGGCT CTGACAGCGC GGCAAGTGAT GGAGACTCAG AAAGCCTTAG AGCACATTCA 
GATGAGGACT CTCTGCTGCG ATGGCTGAAT ACCTTTCGGC GCACTGGGAA TGTGACTCGA 
AGCGGACAGA ATGGGAACCA AAGCTGGAGG GCCGTGAGTC GAACAAACCC GAACAGTGGC 
GAGTTCGGGT TTAGCCTGGA AATCCACATA AACCCTGACA ACAGAGGTTC TGAAATGCAT 
GGAGAGGACT CGACGGACAT CCCTCTGTCA GGTGTTAACA GAGAGCATAG GCAGCAAAGA 
CCATCGAGTC CTGTGGCTAG GCGAACCAGA AGCCAGACCT CCATGAGCTC CAGTGGTCCT 
AGGGGCAGGC GTGGTGCGAG GAGGCAGGGT TCTGTGCAAG GGTCATTTGC AACTCTGGGA 
AGGTTAAGGA ATGGAATTGG AGTAGCCCTT GGTGTTCCCA GAGTTAGTGC CCCTCGCACT 
AATGTTATTA ATTCCCACAC CAACCAGTCA GATGGTAGTA CTCTCAGGCA AGGTGGACGG 
CAGCGGTTTG GAGCAGCACA TATTTGGGAA AACGGGGCTA GAAGTAATGT TACAGTGAGG 
AACACAAACC AAAGATTAGA GCCAATAAGA TTACGTCCTG CTTTCAGTAG TCGAAGCCGG 
TCCCCCATTC AGAGACAGAA TGGTACTGTT CATCATAATT CCCAAAGGCA GGGTAGACCA 
GTACAGCAAA CGGGTAGAAA CAGGTCTGTT AGGCACAGAG GTGTAACGCG GGTCTTTTTA 
GAACAAGGTA GAGAACACAG AGGCACTGAT TACACCCCGC TGTCCAACTC AAGACTTGTG 
TCAAGAATAA CAGTAGAAGA GGGAGAGTCT AGCAGGTCCT CAGCTGCCAC CCAGCGTCAT 
CCAGCAATCA CACTGGACCT GCAAGTCAGG AGGATTCGGC CTGGAGAAAC TAGAGATAGG 
GATAGCATTG CAAATAGAAC CCGATCTAGG GCGGGGCTGG CAGAAAGTAC GGTTGAGAGC 
ACCAGTGGGG GCTTCCACAG AACTATCTCT CACTTAGAGC GCTCAGGTGT CCGAACCTAC 
GTGAGCACCA TCACAGTTCC TCTTCGCAGG ATTTCTGAGA ATGACGTGGT CGAGCCATCC 
TCCGTGGCTC TTCGGTCAAT TTTAAGGCAG ATCATGACTG GGTTTGGAGA GCTGAGTTCT 
CTAATGGAGG TGGAGCCTGC GTCAGAGAAT CAAAGCAATG GCCAACGTTT ACCAGAGGTG 
TACCTAGAAC TGAGTAATGG AGATGCAGCC GATGACAGTG GTCAGCATGG AAGAGCGTCC 
TCACAGGCCA GTCAGGCCCA GGACGGTGCT GAAATGCTAG CTGTGAGGGA GCCCGCCCCG 
CCCCAGGCTC GCCCCAGTGG CAGTAGAAGT AGAAGGCAGT TAGGAAGGGC GGACAGCGTA 
GTTGAGGCTG GAACATTACC CATTCTGCGC CTTGCTCACT TCTTCTTACT GAATGAAGGC 
GATGATGATC CAATTCGTGG TTTAACCAAA GAGCAGATTG ACAATCTTTC TACCAGGAGT 
TATGAACAAG ACGGGGTTGA CAGTGAGTTA GGTAAGGTGT GTAGCGTGTG CATCAGTGAC 
TACGTGGCTG GGAACAAGCT CCGGCAGCTG CCTTGTCTGC ACGAGTTTCA CATTCACTGT 
ATTGATCGCT GGCTGTCGGA GAACTGCACC TGCCCCGTCT GTCGGCGGCC TGTCTTAGAA 
TTCGGAGCCA CAAGCAGTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf6 ( NM_028774.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083050.1 Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05990
Clone ID Related Accession (Same CDS sequence)	NM_001256085.1 , NM_001256087.1 , NM_028774.3
Accession Version	NM_028774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY323318.1, AK004745.1 and AV586321.1. On Jan 4, 2012 this sequence version replaced NM_028774.2. Transcript Variant: This variant (1) encodes the predominant isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK004745.1, AY039004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGATCCGT CTAGATCTAG ATCAGGTGGG AGTGGAGAAG AGAGCTCATT TCAAGAAAAT 
GAGAGAAGAT GGCAGCAAGA ACGTCTCCAC AGAGAAGAAG CCTATTATCA GTTTATTAAT 
GAGCTCAGTG ATGAAGATTA TCGGCTAATG AGAGACCATA ATCTTTTAGG CACCCCTGGA 
GAAATAACAT CAGAGGAGCT GCAGCAGCGC TTAGAGAGAG CAAAGGAACA GCTGGCATCT 
CAGCCTGGCT CTGACAGCGC GGCAAGTGAT GGAGACTCAG AAAGCCTTAG AGCACATTCA 
GATGAGGACT CTCTGCTGCG ATGGCTGAAT ACCTTTCGGC GCACTGGGAA TGTGACTCGA 
AGCGGACAGA ATGGGAACCA AAGCTGGAGG GCCGTGAGTC GAACAAACCC GAACAGTGGC 
GAGTTCGGGT TTAGCCTGGA AATCCACATA AACCCTGACA ACAGAGGTTC TGAAATGCAT 
GGAGAGGACT CGACGGACAT CCCTCTGTCA GGTGTTAACA GAGAGCATAG GCAGCAAAGA 
CCATCGAGTC CTGTGGCTAG GCGAACCAGA AGCCAGACCT CCATGAGCTC CAGTGGTCCT 
AGGGGCAGGC GTGGTGCGAG GAGGCAGGGT TCTGTGCAAG GGTCATTTGC AACTCTGGGA 
AGGTTAAGGA ATGGAATTGG AGTAGCCCTT GGTGTTCCCA GAGTTAGTGC CCCTCGCACT 
AATGTTATTA ATTCCCACAC CAACCAGTCA GATGGTAGTA CTCTCAGGCA AGGTGGACGG 
CAGCGGTTTG GAGCAGCACA TATTTGGGAA AACGGGGCTA GAAGTAATGT TACAGTGAGG 
AACACAAACC AAAGATTAGA GCCAATAAGA TTACGTCCTG CTTTCAGTAG TCGAAGCCGG 
TCCCCCATTC AGAGACAGAA TGGTACTGTT CATCATAATT CCCAAAGGCA GGGTAGACCA 
GTACAGCAAA CGGGTAGAAA CAGGTCTGTT AGGCACAGAG GTGTAACGCG GGTCTTTTTA 
GAACAAGGTA GAGAACACAG AGGCACTGAT TACACCCCGC TGTCCAACTC AAGACTTGTG 
TCAAGAATAA CAGTAGAAGA GGGAGAGTCT AGCAGGTCCT CAGCTGCCAC CCAGCGTCAT 
CCAGCAATCA CACTGGACCT GCAAGTCAGG AGGATTCGGC CTGGAGAAAC TAGAGATAGG 
GATAGCATTG CAAATAGAAC CCGATCTAGG GCGGGGCTGG CAGAAAGTAC GGTTGAGAGC 
ACCAGTGGGG GCTTCCACAG AACTATCTCT CACTTAGAGC GCTCAGGTGT CCGAACCTAC 
GTGAGCACCA TCACAGTTCC TCTTCGCAGG ATTTCTGAGA ATGACGTGGT CGAGCCATCC 
TCCGTGGCTC TTCGGTCAAT TTTAAGGCAG ATCATGACTG GGTTTGGAGA GCTGAGTTCT 
CTAATGGAGG TGGAGCCTGC GTCAGAGAAT CAAAGCAATG GCCAACGTTT ACCAGAGGTG 
TACCTAGAAC TGAGTAATGG AGATGCAGCC GATGACAGTG GTCAGCATGG AAGAGCGTCC 
TCACAGGCCA GTCAGGCCCA GGACGGTGCT GAAATGCTAG CTGTGAGGGA GCCCGCCCCG 
CCCCAGGCTC GCCCCAGTGG CAGTAGAAGT AGAAGGCAGT TAGGAAGGGC GGACAGCGTA 
GTTGAGGCTG GAACATTACC CATTCTGCGC CTTGCTCACT TCTTCTTACT GAATGAAGGC 
GATGATGATC CAATTCGTGG TTTAACCAAA GAGCAGATTG ACAATCTTTC TACCAGGAGT 
TATGAACAAG ACGGGGTTGA CAGTGAGTTA GGTAAGGTGT GTAGCGTGTG CATCAGTGAC 
TACGTGGCTG GGAACAAGCT CCGGCAGCTG CCTTGTCTGC ACGAGTTTCA CATTCACTGT 
ATTGATCGCT GGCTGTCGGA GAACTGCACC TGCCCCGTCT GTCGGCGGCC TGTCTTAGAA 
TTCGGAGCCA CAAGCAGTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083050.1
CDS	340..2343
Translation	MDPSRSRSGGSGEESSFQENERRWQQERLHREEAYYQFINELSDEDYRLMRDHNLLGTPGEITSEELQQRLERAKEQLASQPGSDSAASDGDSESLRAHSDEDSLLRWLNTFRRTGNVTRSGQNGNQSWRAVSRTNPNSGEFGFSLEIHINPDNRGSEMHGEDSTDIPLSGVNREHRQQRPSSPVARRTRSQTSMSSSGPRGRRGARRQGSVQGSFATLGRLRNGIGVALGVPRVSAPRTNVINSHTNQSDGSTLRQGGRQRFGAAHIWENGARSNVTVRNTNQRLEPIRLRPAFSSRSRSPIQRQNGTVHHNSQRQGRPVQQTGRNRSVRHRGVTRVFLEQGREHRGTDYTPLSNSRLVSRITVEEGESSRSSAATQRHPAITLDLQVRRIRPGETRDRDSIANRTRSRAGLAESTVESTSGGFHRTISHLERSGVRTYVSTITVPLRRISENDVVEPSSVALRSILRQIMTGFGELSSLMEVEPASENQSNGQRLPEVYLELSNGDAADDSGQHGRASSQASQAQDGAEMLAVREPAPPQARPSGSRSRRQLGRADSVVEAGTLPILRLAHFFLLNEGDDDPIRGLTKEQIDNLSTRSYEQDGVDSELGKVCSVCISDYVAGNKLRQLPCLHEFHIHCIDRWLSENCTCPVCRRPVLEFGATSSG

Target ORF information:

RefSeq Version	NM_028774.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028774.3

ORF Insert Sequence:

ATGGATCCGT CTAGATCTAG ATCAGGTGGG AGTGGAGAAG AGAGCTCATT TCAAGAAAAT 
GAGAGAAGAT GGCAGCAAGA ACGTCTCCAC AGAGAAGAAG CCTATTATCA GTTTATTAAT 
GAGCTCAGTG ATGAAGATTA TCGGCTAATG AGAGACCATA ATCTTTTAGG CACCCCTGGA 
GAAATAACAT CAGAGGAGCT GCAGCAGCGC TTAGAGAGAG CAAAGGAACA GCTGGCATCT 
CAGCCTGGCT CTGACAGCGC GGCAAGTGAT GGAGACTCAG AAAGCCTTAG AGCACATTCA 
GATGAGGACT CTCTGCTGCG ATGGCTGAAT ACCTTTCGGC GCACTGGGAA TGTGACTCGA 
AGCGGACAGA ATGGGAACCA AAGCTGGAGG GCCGTGAGTC GAACAAACCC GAACAGTGGC 
GAGTTCGGGT TTAGCCTGGA AATCCACATA AACCCTGACA ACAGAGGTTC TGAAATGCAT 
GGAGAGGACT CGACGGACAT CCCTCTGTCA GGTGTTAACA GAGAGCATAG GCAGCAAAGA 
CCATCGAGTC CTGTGGCTAG GCGAACCAGA AGCCAGACCT CCATGAGCTC CAGTGGTCCT 
AGGGGCAGGC GTGGTGCGAG GAGGCAGGGT TCTGTGCAAG GGTCATTTGC AACTCTGGGA 
AGGTTAAGGA ATGGAATTGG AGTAGCCCTT GGTGTTCCCA GAGTTAGTGC CCCTCGCACT 
AATGTTATTA ATTCCCACAC CAACCAGTCA GATGGTAGTA CTCTCAGGCA AGGTGGACGG 
CAGCGGTTTG GAGCAGCACA TATTTGGGAA AACGGGGCTA GAAGTAATGT TACAGTGAGG 
AACACAAACC AAAGATTAGA GCCAATAAGA TTACGTCCTG CTTTCAGTAG TCGAAGCCGG 
TCCCCCATTC AGAGACAGAA TGGTACTGTT CATCATAATT CCCAAAGGCA GGGTAGACCA 
GTACAGCAAA CGGGTAGAAA CAGGTCTGTT AGGCACAGAG GTGTAACGCG GGTCTTTTTA 
GAACAAGGTA GAGAACACAG AGGCACTGAT TACACCCCGC TGTCCAACTC AAGACTTGTG 
TCAAGAATAA CAGTAGAAGA GGGAGAGTCT AGCAGGTCCT CAGCTGCCAC CCAGCGTCAT 
CCAGCAATCA CACTGGACCT GCAAGTCAGG AGGATTCGGC CTGGAGAAAC TAGAGATAGG 
GATAGCATTG CAAATAGAAC CCGATCTAGG GCGGGGCTGG CAGAAAGTAC GGTTGAGAGC 
ACCAGTGGGG GCTTCCACAG AACTATCTCT CACTTAGAGC GCTCAGGTGT CCGAACCTAC 
GTGAGCACCA TCACAGTTCC TCTTCGCAGG ATTTCTGAGA ATGACGTGGT CGAGCCATCC 
TCCGTGGCTC TTCGGTCAAT TTTAAGGCAG ATCATGACTG GGTTTGGAGA GCTGAGTTCT 
CTAATGGAGG TGGAGCCTGC GTCAGAGAAT CAAAGCAATG GCCAACGTTT ACCAGAGGTG 
TACCTAGAAC TGAGTAATGG AGATGCAGCC GATGACAGTG GTCAGCATGG AAGAGCGTCC 
TCACAGGCCA GTCAGGCCCA GGACGGTGCT GAAATGCTAG CTGTGAGGGA GCCCGCCCCG 
CCCCAGGCTC GCCCCAGTGG CAGTAGAAGT AGAAGGCAGT TAGGAAGGGC GGACAGCGTA 
GTTGAGGCTG GAACATTACC CATTCTGCGC CTTGCTCACT TCTTCTTACT GAATGAAGGC 
GATGATGATC CAATTCGTGG TTTAACCAAA GAGCAGATTG ACAATCTTTC TACCAGGAGT 
TATGAACAAG ACGGGGTTGA CAGTGAGTTA GGTAAGGTGT GTAGCGTGTG CATCAGTGAC 
TACGTGGCTG GGAACAAGCT CCGGCAGCTG CCTTGTCTGC ACGAGTTTCA CATTCACTGT 
ATTGATCGCT GGCTGTCGGA GAACTGCACC TGCCCCGTCT GTCGGCGGCC TGTCTTAGAA 
TTCGGAGCCA CAAGCAGTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf6 ( NM_001256086.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243015.1 Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 4, mRNA.

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CloneID	OMu05991
Clone ID Related Accession (Same CDS sequence)	NM_001256086.1
Accession Version	NM_001256086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113316.14. Transcript Variant: This variant (4) differs in the 5' UTR and coding region which includes an upstream in-frame AUG start codon, compared to variant 1. The resulting isoform (2) is longer at the N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BE531748.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGAAT TAAAGGAAAC TAGAAGTCTC AGGATGGATC CGTCTAGATC TAGATCAGGT 
GGGAGTGGAG AAGAGAGCTC ATTTCAAGAA AATGAGAGAA GATGGCAGCA AGAACGTCTC 
CACAGAGAAG AAGCCTATTA TCAGTTTATT AATGAGCTCA GTGATGAAGA TTATCGGCTA 
ATGAGAGACC ATAATCTTTT AGGCACCCCT GGAGAAATAA CATCAGAGGA GCTGCAGCAG 
CGCTTAGAGA GAGCAAAGGA ACAGCTGGCA TCTCAGCCTG GCTCTGACAG CGCGGCAAGT 
GATGGAGACT CAGAAAGCCT TAGAGCACAT TCAGATGAGG ACTCTCTGCT GCGATGGCTG 
AATACCTTTC GGCGCACTGG GAATGTGACT CGAAGCGGAC AGAATGGGAA CCAAAGCTGG 
AGGGCCGTGA GTCGAACAAA CCCGAACAGT GGCGAGTTCG GGTTTAGCCT GGAAATCCAC 
ATAAACCCTG ACAACAGAGG TTCTGAAATG CATGGAGAGG ACTCGACGGA CATCCCTCTG 
TCAGGTGTTA ACAGAGAGCA TAGGCAGCAA AGACCATCGA GTCCTGTGGC TAGGCGAACC 
AGAAGCCAGA CCTCCATGAG CTCCAGTGGT CCTAGGGGCA GGCGTGGTGC GAGGAGGCAG 
GGTTCTGTGC AAGGGTCATT TGCAACTCTG GGAAGGTTAA GGAATGGAAT TGGAGTAGCC 
CTTGGTGTTC CCAGAGTTAG TGCCCCTCGC ACTAATGTTA TTAATTCCCA CACCAACCAG 
TCAGATGGTA GTACTCTCAG GCAAGGTGGA CGGCAGCGGT TTGGAGCAGC ACATATTTGG 
GAAAACGGGG CTAGAAGTAA TGTTACAGTG AGGAACACAA ACCAAAGATT AGAGCCAATA 
AGATTACGTC CTGCTTTCAG TAGTCGAAGC CGGTCCCCCA TTCAGAGACA GAATGGTACT 
GTTCATCATA ATTCCCAAAG GCAGGGTAGA CCAGTACAGC AAACGGGTAG AAACAGGTCT 
GTTAGGCACA GAGGTGTAAC GCGGGTCTTT TTAGAACAAG GTAGAGAACA CAGAGGCACT 
GATTACACCC CGCTGTCCAA CTCAAGACTT GTGTCAAGAA TAACAGTAGA AGAGGGAGAG 
TCTAGCAGGT CCTCAGCTGC CACCCAGCGT CATCCAGCAA TCACACTGGA CCTGCAAGTC 
AGGAGGATTC GGCCTGGAGA AACTAGAGAT AGGGATAGCA TTGCAAATAG AACCCGATCT 
AGGGCGGGGC TGGCAGAAAG TACGGTTGAG AGCACCAGTG GGGGCTTCCA CAGAACTATC 
TCTCACTTAG AGCGCTCAGG TGTCCGAACC TACGTGAGCA CCATCACAGT TCCTCTTCGC 
AGGATTTCTG AGAATGACGT GGTCGAGCCA TCCTCCGTGG CTCTTCGGTC AATTTTAAGG 
CAGATCATGA CTGGGTTTGG AGAGCTGAGT TCTCTAATGG AGGTGGAGCC TGCGTCAGAG 
AATCAAAGCA ATGGCCAACG TTTACCAGAG GTGTACCTAG AACTGAGTAA TGGAGATGCA 
GCCGATGACA GTGGTCAGCA TGGAAGAGCG TCCTCACAGG CCAGTCAGGC CCAGGACGGT 
GCTGAAATGC TAGCTGTGAG GGAGCCCGCC CCGCCCCAGG CTCGCCCCAG TGGCAGTAGA 
AGTAGAAGGC AGTTAGGAAG GGCGGACAGC GTAGTTGAGG CTGGAACATT ACCCATTCTG 
CGCCTTGCTC ACTTCTTCTT ACTGAATGAA GGCGATGATG ATCCAATTCG TGGTTTAACC 
AAAGAGCAGA TTGACAATCT TTCTACCAGG AGTTATGAAC AAGACGGGGT TGACAGTGAG 
TTAGGTAAGG TGTGTAGCGT GTGCATCAGT GACTACGTGG CTGGGAACAA GCTCCGGCAG 
CTGCCTTGTC TGCACGAGTT TCACATTCAC TGTATTGATC GCTGGCTGTC GGAGAACTGC 
ACCTGCCCCG TCTGTCGGCG GCCTGTCTTA GAATTCGGAG CCACAAGCAG TGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243015.1
CDS	179..2215
Translation	MLELKETRSLRMDPSRSRSGGSGEESSFQENERRWQQERLHREEAYYQFINELSDEDYRLMRDHNLLGTPGEITSEELQQRLERAKEQLASQPGSDSAASDGDSESLRAHSDEDSLLRWLNTFRRTGNVTRSGQNGNQSWRAVSRTNPNSGEFGFSLEIHINPDNRGSEMHGEDSTDIPLSGVNREHRQQRPSSPVARRTRSQTSMSSSGPRGRRGARRQGSVQGSFATLGRLRNGIGVALGVPRVSAPRTNVINSHTNQSDGSTLRQGGRQRFGAAHIWENGARSNVTVRNTNQRLEPIRLRPAFSSRSRSPIQRQNGTVHHNSQRQGRPVQQTGRNRSVRHRGVTRVFLEQGREHRGTDYTPLSNSRLVSRITVEEGESSRSSAATQRHPAITLDLQVRRIRPGETRDRDSIANRTRSRAGLAESTVESTSGGFHRTISHLERSGVRTYVSTITVPLRRISENDVVEPSSVALRSILRQIMTGFGELSSLMEVEPASENQSNGQRLPEVYLELSNGDAADDSGQHGRASSQASQAQDGAEMLAVREPAPPQARPSGSRSRRQLGRADSVVEAGTLPILRLAHFFLLNEGDDDPIRGLTKEQIDNLSTRSYEQDGVDSELGKVCSVCISDYVAGNKLRQLPCLHEFHIHCIDRWLSENCTCPVCRRPVLEFGATSSG

Target ORF information:

RefSeq Version	NM_001256086.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256086.1

ORF Insert Sequence:

ATGCTGGAAT TAAAGGAAAC TAGAAGTCTC AGGATGGATC CGTCTAGATC TAGATCAGGT 
GGGAGTGGAG AAGAGAGCTC ATTTCAAGAA AATGAGAGAA GATGGCAGCA AGAACGTCTC 
CACAGAGAAG AAGCCTATTA TCAGTTTATT AATGAGCTCA GTGATGAAGA TTATCGGCTA 
ATGAGAGACC ATAATCTTTT AGGCACCCCT GGAGAAATAA CATCAGAGGA GCTGCAGCAG 
CGCTTAGAGA GAGCAAAGGA ACAGCTGGCA TCTCAGCCTG GCTCTGACAG CGCGGCAAGT 
GATGGAGACT CAGAAAGCCT TAGAGCACAT TCAGATGAGG ACTCTCTGCT GCGATGGCTG 
AATACCTTTC GGCGCACTGG GAATGTGACT CGAAGCGGAC AGAATGGGAA CCAAAGCTGG 
AGGGCCGTGA GTCGAACAAA CCCGAACAGT GGCGAGTTCG GGTTTAGCCT GGAAATCCAC 
ATAAACCCTG ACAACAGAGG TTCTGAAATG CATGGAGAGG ACTCGACGGA CATCCCTCTG 
TCAGGTGTTA ACAGAGAGCA TAGGCAGCAA AGACCATCGA GTCCTGTGGC TAGGCGAACC 
AGAAGCCAGA CCTCCATGAG CTCCAGTGGT CCTAGGGGCA GGCGTGGTGC GAGGAGGCAG 
GGTTCTGTGC AAGGGTCATT TGCAACTCTG GGAAGGTTAA GGAATGGAAT TGGAGTAGCC 
CTTGGTGTTC CCAGAGTTAG TGCCCCTCGC ACTAATGTTA TTAATTCCCA CACCAACCAG 
TCAGATGGTA GTACTCTCAG GCAAGGTGGA CGGCAGCGGT TTGGAGCAGC ACATATTTGG 
GAAAACGGGG CTAGAAGTAA TGTTACAGTG AGGAACACAA ACCAAAGATT AGAGCCAATA 
AGATTACGTC CTGCTTTCAG TAGTCGAAGC CGGTCCCCCA TTCAGAGACA GAATGGTACT 
GTTCATCATA ATTCCCAAAG GCAGGGTAGA CCAGTACAGC AAACGGGTAG AAACAGGTCT 
GTTAGGCACA GAGGTGTAAC GCGGGTCTTT TTAGAACAAG GTAGAGAACA CAGAGGCACT 
GATTACACCC CGCTGTCCAA CTCAAGACTT GTGTCAAGAA TAACAGTAGA AGAGGGAGAG 
TCTAGCAGGT CCTCAGCTGC CACCCAGCGT CATCCAGCAA TCACACTGGA CCTGCAAGTC 
AGGAGGATTC GGCCTGGAGA AACTAGAGAT AGGGATAGCA TTGCAAATAG AACCCGATCT 
AGGGCGGGGC TGGCAGAAAG TACGGTTGAG AGCACCAGTG GGGGCTTCCA CAGAACTATC 
TCTCACTTAG AGCGCTCAGG TGTCCGAACC TACGTGAGCA CCATCACAGT TCCTCTTCGC 
AGGATTTCTG AGAATGACGT GGTCGAGCCA TCCTCCGTGG CTCTTCGGTC AATTTTAAGG 
CAGATCATGA CTGGGTTTGG AGAGCTGAGT TCTCTAATGG AGGTGGAGCC TGCGTCAGAG 
AATCAAAGCA ATGGCCAACG TTTACCAGAG GTGTACCTAG AACTGAGTAA TGGAGATGCA 
GCCGATGACA GTGGTCAGCA TGGAAGAGCG TCCTCACAGG CCAGTCAGGC CCAGGACGGT 
GCTGAAATGC TAGCTGTGAG GGAGCCCGCC CCGCCCCAGG CTCGCCCCAG TGGCAGTAGA 
AGTAGAAGGC AGTTAGGAAG GGCGGACAGC GTAGTTGAGG CTGGAACATT ACCCATTCTG 
CGCCTTGCTC ACTTCTTCTT ACTGAATGAA GGCGATGATG ATCCAATTCG TGGTTTAACC 
AAAGAGCAGA TTGACAATCT TTCTACCAGG AGTTATGAAC AAGACGGGGT TGACAGTGAG 
TTAGGTAAGG TGTGTAGCGT GTGCATCAGT GACTACGTGG CTGGGAACAA GCTCCGGCAG 
CTGCCTTGTC TGCACGAGTT TCACATTCAC TGTATTGATC GCTGGCTGTC GGAGAACTGC 
ACCTGCCCCG TCTGTCGGCG GCCTGTCTTA GAATTCGGAG CCACAAGCAG TGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Islet ?-, ?-, and ?-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor.
Diabetes62(3)875-86(2013 Mar)
Hunter CS,Dixit S,Cohen T,Ediger B,Wilcox C,Ferreira M,Westphal H,Stein R,May CL

Distinct gene loci control the host response to influenza H1N1 virus infection in a time-dependent manner.
BMC genomics13411(2012 Aug)
Nedelko T,Kollmus H,Klawonn F,Spijker S,Lu L,He?man M,Alberts R,Williams RW,Schughart K

The ubiquitin ligase Rnf6 regulates local LIM kinase 1 levels in axonal growth cones.
Genes & development19(19)2307-19(2005 Oct)
Tursun B,Schl?ter A,Peters MA,Viehweger B,Ostendorff HP,Soosairajah J,Drung A,Bossenz M,Johnsen SA,Schweizer M,Bernard O,Bach I

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Gene control in germinal differentiation: RNF6, a transcription regulatory protein in the mouse sertoli cell.
Molecular and cellular biology22(10)3488-96(2002 May)
Lopez P,Vidal F,Martin L,Lopez-Fernandez LA,Rual JF,Rosen BS,Cuzin F,Rassoulzadegan M

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Rnf6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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