Ndufaf7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndufaf7
Entrez Gene ID	73694
Full Name	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 7
Synonyms	2410091C18Rik,AL033374,PRO1853
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 7 Names protein arginine methyltransferase NDUFAF7, mitochondrial NADH dehydrogenase [ubiquinone] complex I, assembly factor 7 protein midA homolog, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_028611.3	NP_082887.2	protein arginine methyltransferase NDUFAF7, mitochondrial precursor
XM_006524995.1	XP_006525058.1	protein arginine methyltransferase NDUFAF7, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ndufaf7	NP_001070231.1
Caenorhabditis elegans (roundworm)	ZK1128.1	NP_499246.2
Neurospora crassa	NCU00183	XP_956406.1
Canis lupus familiaris (dog)	NDUFAF7	XP_532933.2
Rattus norvegicus (Norway rat)	Ndufaf7	NP_001008319.1
Homo sapiens (human)	NDUFAF7	NP_653337.1
Pan troglodytes (chimpanzee)	NDUFAF7	XP_001167268.1
	LOC709473	XP_001108260.2
Bos taurus (cattle)	NDUFAF7	NP_001094519.2
Drosophila melanogaster (fruit fly)	CG17726	NP_650054.2
Xenopus tropicalis (tropical clawed frog)	ndufaf7	NP_001016145.1
Mus musculus (house mouse)	Ndufaf7	NP_082887.2
Gallus gallus (chicken)	C2ORF56	XP_419525.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004909	XP_315000.4
Magnaporthe oryzae (rice blast fungus)	MGG_06414	XP_003717155.1
Arabidopsis thaliana (thale cress)	AT3G28700	NP_189511.2
Oryza sativa (rice)	Os01g0588800	NP_001043438.1

Gene Ontology
Bibliography

Related articles in PubMed

The arginine methyltransferase NDUFAF7 is essential for complex I assembly and early vertebrate embryogenesis.
Zurita Rend?n O, Silva Neiva L, Sasarman F, Shoubridge EA
Human molecular genetics23(19)5159-70(2014 Oct)

Host genetic variation affects resistance to infection with a highly pathogenic H5N1 influenza A virus in mice.
Boon AC, deBeauchamp J, Hollmann A, Luke J, Kotb M, Rowe S, Finkelstein D, Neale G, Lu L, Williams RW, Webby RJ
Journal of virology83(20)10417-26(2009 Oct)

A neurogenomics approach to gene expression analysis in the developing brain.
Jensen P, Magdaleno S, Lehman KM, Rice DS, Lavallie ER, Collins-Racie L, McCoy JM, Curran T
Brain research. Molecular brain research132(2)116-27(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NDUFAF7 functions to methylate NDUFS2 after it assembles into a complex I, stabilizing an early intermediate in the assembly pathway, and that this function is essential for normal vertebrate development.
Title: The arginine methyltransferase NDUFAF7 is essential for complex I assembly and early vertebrate embryogenesis.

The following Ndufaf7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufaf7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13045	NM_028611.3 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase complex assembly factor 7 (Ndufaf7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
OMu54083	XM_006524995.1 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase complex assembly factor 7 (Ndufaf7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufaf7 ( NM_028611.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082887.2 Mus musculus NADH:ubiquinone oxidoreductase complex assembly factor 7 (Ndufaf7), mRNA.

Price & Availability↑

CloneID	OMu13045
Clone ID Related Accession (Same CDS sequence)	NM_028611.3
Accession Version	NM_028611.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-20
Organism	Mus musculus(house mouse)
Product	protein arginine methyltransferase NDUFAF7, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX236822.1, AK010752.1, CF617214.1 and AC154274.1. On Oct 31, 2007 this sequence version replaced NM_028611.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.149680.1, AK010752.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGCTT TGGTGAGACG CTGCGTGGCC CGCGCGGGCC TTCCCTGTAT TTGGAGAGGG 
AAATGCTACA GCTCCGGGAA TGAGCCGGCA GAAAGCAACC AGGTGACACC GATGCTGCGG 
CATCTTATGT ACAAAATAAA GTCTACAGGT CCCATCACTG TGGCCGAGTA CATGAAGGAG 
GTTTTGACTA ACCCAGCCAA GGGATATTAT GTGCATCAGG ATATGCTAGG AGAAAAAGGA 
GACTTTATTA CTTCACCTGA AATAAGCCAA ATCTTCGGGG AGCTACTAGG GGTGTGGTTT 
GTTAGTGAAT GGATAGCCTC TGGAAAAAGC CCAGCATTCC AGCTGGTGGA ACTTGGCCCT 
GGTCGGGGCA CCCTCACAGC AGATATTTTG AGGGTGTTCA GTCAGCTTGG GTCTGTGCTA 
AAGACCTGTG CCATCTCAAT ACACCTTGTA GAGGTGAGCC AGAAACTAAG TGAGATCCAA 
GCACTGACAC TGGCTGAGGA GAAGGTCCCA TTGGAGAGAG ACGCTGAATC CCTGGTGTAC 
ATGAAAGGTG TCACAAAGTC TGGGATTCCA GTCTCCTGGT ACCGAGACCT GAAGGATGTT 
CCTGAAGGAT ACAGCTTGTA CCTCGCCCAT GAGTTTTTTG ATGTTCTTCC TGTGCATAAA 
TTTCAGAAAA CACCACGCGG ATGGAGAGAA GTGTTTGTTG ATGTCGACCC ACAAGCTTCT 
GATAAACTGA GGTTCGTCTT GGCGCCTTGT GCGACCCCTG CAGAAGCCTT CATTCAACGT 
GATGAAAGGA GGGAACATGT GGAAGTATGT CCCGATGCTG GGGTTATCAT CCAAGAACTG 
TCCCAGCGCA TTGCGTCAAC TGGAGGTGCA GCCCTGATTG CGGATTACGG TCACGATGGC 
ACAAAGACAG ACACCTTGAG AGGGTTTTAT GGACACCAGC TTCACGATGT CTTAATCGCT 
CCTGGAACAG CAGACCTGAC AGCTGATGTG GACTTCAGTT ACCTGCGCAG AATGGCACAA 
GGAAAAGTAG CCTCTCTGGG TCCAGTGGAA CAGCGAACAT TTTTAAAAAA CATGGGCATT 
GATGTCCGGC TGAAGGTTCT CTTGGATAAA GCAGGTGAGC CATCTGCGAA GCAGCAGCTA 
CTTGGAGGGT ATGATATGTT AATGAATCCT CAGAAGATGG GAGAAAGATT TCACTTCTTT 
GCCTTGCTAC CTCATCAGAG ACTTCATGGG GGGAGTCAAG AAAGGAATGC CTGCCAGTCA 
AAAACCCCCT CCTCCTCTGT AGCTGGGTTT GATGAACTTG TTTGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082887.2
CDS	31..1341
Translation	MNALVRRCVARAGLPCIWRGKCYSSGNEPAESNQVTPMLRHLMYKIKSTGPITVAEYMKEVLTNPAKGYYVHQDMLGEKGDFITSPEISQIFGELLGVWFVSEWIASGKSPAFQLVELGPGRGTLTADILRVFSQLGSVLKTCAISIHLVEVSQKLSEIQALTLAEEKVPLERDAESLVYMKGVTKSGIPVSWYRDLKDVPEGYSLYLAHEFFDVLPVHKFQKTPRGWREVFVDVDPQASDKLRFVLAPCATPAEAFIQRDERREHVEVCPDAGVIIQELSQRIASTGGAALIADYGHDGTKTDTLRGFYGHQLHDVLIAPGTADLTADVDFSYLRRMAQGKVASLGPVEQRTFLKNMGIDVRLKVLLDKAGEPSAKQQLLGGYDMLMNPQKMGERFHFFALLPHQRLHGGSQERNACQSKTPSSSVAGFDELVWQ

Target ORF information:

RefSeq Version	NM_028611.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase complex assembly factor 7 (Ndufaf7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028611.3

ORF Insert Sequence:

ATGAATGCTT TGGTGAGACG CTGCGTGGCC CGCGCGGGCC TTCCCTGTAT TTGGAGAGGG 
AAATGCTACA GCTCCGGGAA TGAGCCGGCA GAAAGCAACC AGGTGACACC GATGCTGCGG 
CATCTTATGT ACAAAATAAA GTCTACAGGT CCCATCACTG TGGCCGAGTA CATGAAGGAG 
GTTTTGACTA ACCCAGCCAA GGGATATTAT GTGCATCAGG ATATGCTAGG AGAAAAAGGA 
GACTTTATTA CTTCACCTGA AATAAGCCAA ATCTTCGGGG AGCTACTAGG GGTGTGGTTT 
GTTAGTGAAT GGATAGCCTC TGGAAAAAGC CCAGCATTCC AGCTGGTGGA ACTTGGCCCT 
GGTCGGGGCA CCCTCACAGC AGATATTTTG AGGGTGTTCA GTCAGCTTGG GTCTGTGCTA 
AAGACCTGTG CCATCTCAAT ACACCTTGTA GAGGTGAGCC AGAAACTAAG TGAGATCCAA 
GCACTGACAC TGGCTGAGGA GAAGGTCCCA TTGGAGAGAG ACGCTGAATC CCTGGTGTAC 
ATGAAAGGTG TCACAAAGTC TGGGATTCCA GTCTCCTGGT ACCGAGACCT GAAGGATGTT 
CCTGAAGGAT ACAGCTTGTA CCTCGCCCAT GAGTTTTTTG ATGTTCTTCC TGTGCATAAA 
TTTCAGAAAA CACCACGCGG ATGGAGAGAA GTGTTTGTTG ATGTCGACCC ACAAGCTTCT 
GATAAACTGA GGTTCGTCTT GGCGCCTTGT GCGACCCCTG CAGAAGCCTT CATTCAACGT 
GATGAAAGGA GGGAACATGT GGAAGTATGT CCCGATGCTG GGGTTATCAT CCAAGAACTG 
TCCCAGCGCA TTGCGTCAAC TGGAGGTGCA GCCCTGATTG CGGATTACGG TCACGATGGC 
ACAAAGACAG ACACCTTGAG AGGGTTTTAT GGACACCAGC TTCACGATGT CTTAATCGCT 
CCTGGAACAG CAGACCTGAC AGCTGATGTG GACTTCAGTT ACCTGCGCAG AATGGCACAA 
GGAAAAGTAG CCTCTCTGGG TCCAGTGGAA CAGCGAACAT TTTTAAAAAA CATGGGCATT 
GATGTCCGGC TGAAGGTTCT CTTGGATAAA GCAGGTGAGC CATCTGCGAA GCAGCAGCTA 
CTTGGAGGGT ATGATATGTT AATGAATCCT CAGAAGATGG GAGAAAGATT TCACTTCTTT 
GCCTTGCTAC CTCATCAGAG ACTTCATGGG GGGAGTCAAG AAAGGAATGC CTGCCAGTCA 
AAAACCCCCT CCTCCTCTGT AGCTGGGTTT GATGAACTTG TTTGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufaf7 ( XM_006524995.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525058.1 Mus musculus NADH:ubiquinone oxidoreductase complex assembly factor 7 (Ndufaf7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54083
Clone ID Related Accession (Same CDS sequence)	XM_006524995.1
Accession Version	XM_006524995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein arginine methyltransferase NDUFAF7, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTT TGGTGAGACG CTGCGTGGCC CGCGCGGGCC TTCCCTGTAT TTGGAGAGGG 
AAATGCTACA GCTCCGGGAA TGAGCCGGCA GAAAGCAACC AGGTGACACC GATGCTGCGG 
CATCTTATGT ACAAAATAAA GTCTACAGGT CCCATCACTG TGGCCGAGTA CATGAAGGAG 
GTTTTGACTA ACCCAGCCAA GGGATATTAT GTGCATCAGG ATATGCTAGG AGAAAAAGGA 
GACTTTATTA CTTCACCTGA AATAAGCCAA ATCTTCGGGG AGCTACTAGG GGTGTGGTTT 
GTTAGTGAAT GGATAGCCTC TGGAAAAAGC CCAGCATTCC AGCTGGTGGA ACTTGGCCCT 
GGTCGGGGCA CCCTCACAGC AGATATTTTG AGGGTGTTCA GTCAGCTTGG GTCTGTGCTA 
AAGACCTGTG CCATCTCAAT ACACCTTGTA GAGGTGAGCC AGAAACTAAG TGAGATCCAA 
GCACTGACAC TGGCTGAGGA GAAGGTCCCA TTGGAGAGAG ACGCTGAATC CCTGGTGTAC 
ATGAAAGGTG TCACAAAGTC TGGGATTCCA GTCTCCTGGT ACCGAGACCT GAAGGATGTT 
CCTGAAGGAT ACAGCTTGTA CCTCGCCCAT GAGTTTTTTG ATGTTCTTCC TGTGCATAAA 
TTTCAGAAAA CACCACGCGG ATGGAGAGAA GTGTTTGTTG ATGTCGACCC ACAAGCTTCT 
GATAAACTGA GGTTCGTCTT GGCGCCTTGT GCGACCCCTG CAGAAGCCTT CATTCAAGGG 
TTTTATGGAC ACCAGCTTCA CGATGTCTTA ATCGCTCCTG GAACAGCAGA CCTGACAGCT 
GATGTGGACT TCAGTTACCT GCGCAGAATG GCACAAGGAA AAGTAGCCTC TCTGGGTCCA 
GTGGAACAGC GAACATTTTT AAAAAACATG GGCATTGATG TCCGGCTGAA GGTTCTCTTG 
GATAAAGCAG GTGAGCCATC TGCGAAGCAG CAGCTACTTG GAGGGTATGA TATGTTAATG 
AATCCTCAGA AGATGGGAGA AAGATTTCAC TTCTTTGCCT TGCTACCTCA TCAGAGACTT 
CATGGGGGGA GTCAAGAAAG GAATGCCTGC CAGTCAAAAA CCCCCTCCTC CTCTGTAGCT 
GGGTTTGATG AACTTGTTTG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525058.1
CDS	60..1226
Translation	MNALVRRCVARAGLPCIWRGKCYSSGNEPAESNQVTPMLRHLMYKIKSTGPITVAEYMKEVLTNPAKGYYVHQDMLGEKGDFITSPEISQIFGELLGVWFVSEWIASGKSPAFQLVELGPGRGTLTADILRVFSQLGSVLKTCAISIHLVEVSQKLSEIQALTLAEEKVPLERDAESLVYMKGVTKSGIPVSWYRDLKDVPEGYSLYLAHEFFDVLPVHKFQKTPRGWREVFVDVDPQASDKLRFVLAPCATPAEAFIQGFYGHQLHDVLIAPGTADLTADVDFSYLRRMAQGKVASLGPVEQRTFLKNMGIDVRLKVLLDKAGEPSAKQQLLGGYDMLMNPQKMGERFHFFALLPHQRLHGGSQERNACQSKTPSSSVAGFDELVWQ

Target ORF information:

RefSeq Version	XM_006524995.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase complex assembly factor 7 (Ndufaf7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524995.1

ORF Insert Sequence:

ATGAATGCTT TGGTGAGACG CTGCGTGGCC CGCGCGGGCC TTCCCTGTAT TTGGAGAGGG 
AAATGCTACA GCTCCGGGAA TGAGCCGGCA GAAAGCAACC AGGTGACACC GATGCTGCGG 
CATCTTATGT ACAAAATAAA GTCTACAGGT CCCATCACTG TGGCCGAGTA CATGAAGGAG 
GTTTTGACTA ACCCAGCCAA GGGATATTAT GTGCATCAGG ATATGCTAGG AGAAAAAGGA 
GACTTTATTA CTTCACCTGA AATAAGCCAA ATCTTCGGGG AGCTACTAGG GGTGTGGTTT 
GTTAGTGAAT GGATAGCCTC TGGAAAAAGC CCAGCATTCC AGCTGGTGGA ACTTGGCCCT 
GGTCGGGGCA CCCTCACAGC AGATATTTTG AGGGTGTTCA GTCAGCTTGG GTCTGTGCTA 
AAGACCTGTG CCATCTCAAT ACACCTTGTA GAGGTGAGCC AGAAACTAAG TGAGATCCAA 
GCACTGACAC TGGCTGAGGA GAAGGTCCCA TTGGAGAGAG ACGCTGAATC CCTGGTGTAC 
ATGAAAGGTG TCACAAAGTC TGGGATTCCA GTCTCCTGGT ACCGAGACCT GAAGGATGTT 
CCTGAAGGAT ACAGCTTGTA CCTCGCCCAT GAGTTTTTTG ATGTTCTTCC TGTGCATAAA 
TTTCAGAAAA CACCACGCGG ATGGAGAGAA GTGTTTGTTG ATGTCGACCC ACAAGCTTCT 
GATAAACTGA GGTTCGTCTT GGCGCCTTGT GCGACCCCTG CAGAAGCCTT CATTCAAGGG 
TTTTATGGAC ACCAGCTTCA CGATGTCTTA ATCGCTCCTG GAACAGCAGA CCTGACAGCT 
GATGTGGACT TCAGTTACCT GCGCAGAATG GCACAAGGAA AAGTAGCCTC TCTGGGTCCA 
GTGGAACAGC GAACATTTTT AAAAAACATG GGCATTGATG TCCGGCTGAA GGTTCTCTTG 
GATAAAGCAG GTGAGCCATC TGCGAAGCAG CAGCTACTTG GAGGGTATGA TATGTTAATG 
AATCCTCAGA AGATGGGAGA AAGATTTCAC TTCTTTGCCT TGCTACCTCA TCAGAGACTT 
CATGGGGGGA GTCAAGAAAG GAATGCCTGC CAGTCAAAAA CCCCCTCCTC CTCTGTAGCT 
GGGTTTGATG AACTTGTTTG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The arginine methyltransferase NDUFAF7 is essential for complex I assembly and early vertebrate embryogenesis.
Human molecular genetics23(19)5159-70(2014 Oct)
Zurita Rend?n O,Silva Neiva L,Sasarman F,Shoubridge EA

Host genetic variation affects resistance to infection with a highly pathogenic H5N1 influenza A virus in mice.
Journal of virology83(20)10417-26(2009 Oct)
Boon AC,deBeauchamp J,Hollmann A,Luke J,Kotb M,Rowe S,Finkelstein D,Neale G,Lu L,Williams RW,Webby RJ

A neurogenomics approach to gene expression analysis in the developing brain.
Brain research. Molecular brain research132(2)116-27(2004 Dec)
Jensen P,Magdaleno S,Lehman KM,Rice DS,Lavallie ER,Collins-Racie L,McCoy JM,Curran T

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Ndufaf7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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