Hspa12a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspa12a
Entrez Gene ID	73442
Full Name	heat shock protein 12A
Synonyms	1700063D12Rik,AI118035,AI840429,AW048913,AW556406,D5Mgi40,Gm19925,mKIAA0417
General protein information	Preferred Names heat shock protein 12A Names heat shock 70 kDa protein 12A heat shock 70kDa protein 12A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 D2

mRNA and Protein(s)

mRNA	Protein	Name
XM_011247368.2	XP_011245670.1	heat shock 70 kDa protein 12A isoform X2
NM_175199.3	NP_780408.1	heat shock 70 kDa protein 12A isoform 2
XM_006527377.2	XP_006527440.1	heat shock 70 kDa protein 12A isoform X1
XM_006527379.3	XP_006527442.1	heat shock 70 kDa protein 12A isoform X3
XM_006527379.4	XP_006527442.1	heat shock 70 kDa protein 12A isoform X3
NM_001327998.1	NP_001314927.1	heat shock 70 kDa protein 12A isoform 1
XM_011247368.3	XP_011245670.1	heat shock 70 kDa protein 12A isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSPA12A	NP_079291.2
Canis lupus familiaris (dog)	HSPA12A	XP_544034.4
Bos taurus (cattle)	HSPA12A	XP_002698580.3
	HSPA12A	XP_001095628.2
Rattus norvegicus (Norway rat)	Hspa12a	NP_001100915.1
Gallus gallus (chicken)	HSPA12A	XP_004942362.1
Mus musculus (house mouse)	Hspa12a	NP_780408.1
Xenopus tropicalis (tropical clawed frog)	hspa12a	XP_004915788.1
Pan troglodytes (chimpanzee)	HSPA12A	XP_529348.3

Gene Ontology
Bibliography

Related articles in PubMed

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Munton RP, Tweedie-Cullen R, Livingstone-Zatchej M, Weinandy F, Waidelich M, Longo D, Gehrig P, Potthast F, Rutishauser D, Gerrits B, Panse C, Schlapbach R, Mansuy IM
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)

Identification of cathepsin K as a novel marker of adiposity in white adipose tissue.
Chiellini C, Costa M, Novelli SE, Amri EZ, Benzi L, Bertacca A, Cohen P, Del Prato S, Friedman JM, Maffei M
Journal of cellular physiology195(2)309-21(2003 May)

Two Hsp70 family members expressed in atherosclerotic lesions.
Han Z, Truong QA, Park S, Breslow JL
Proceedings of the National Academy of Sciences of the United States of America100(3)1256-61(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HSPA12A and HSPA12B may play roles in genetic susceptibility to atherosclerosis
Title: Two Hsp70 family members expressed in atherosclerotic lesions.

The following Hspa12a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspa12a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu55759	NM_001327998.1 Latest version!	Mus musculus heat shock protein 12A (Hspa12a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu55758	XM_006527377.2 Latest version!	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu04998	NM_175199.3 Latest version!	Mus musculus heat shock protein 12A (Hspa12a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu55760	XM_006527379.4 Latest version!	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu71010	XM_011247368.3 Latest version!	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu55760	XM_006527379.3	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu71010	XM_011247368.2	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspa12a ( XM_006527379.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527442.1 Mus musculus heat shock protein 12A (Hspa12a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu55760
Clone ID Related Accession (Same CDS sequence)	XM_006527379.3 , XM_006527379.4
Accession Version	XM_006527379.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	heat shock 70 kDa protein 12A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527379.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGAT GGGAAGGAGG TGACCCTGGT GTTTCCAACC AGAAGACCCC GACCACCATC 
TTGCTGACAC CAGAGAGGAA GTTCCACAGC TTTGGGTACG CTGCCAGGGA CTTCTACCAT 
GACCTGGACC CCAGTGAGGC CAAGCAATGG CTGTACTTGG AGAAGTTCAA GATGAAACTC 
CACACCACAG GGGACCTCAC CATGGACACA GACTTAACAG CAGCAAATGG CAAGAAGGTC 
AAAGCCCTCG AGATCTTCGC TTACGCCCTG CAGTACTTCA AGGAGCAGGC TCTGAAGGAA 
CTGAGTGACC AGGCAGGGTC GGACTTTGAG AACTCTGACG TCAGATGGGT CATCACAGTG 
CCTGCCATCT GGAAGCAACC AGCCAAGCAG TTCATGAGAG AAGCTGCCTA CCAGGCAGGC 
CTGGCCTCCC CCGAGAACTC GGAGCAGCTC ATCATTGCCC TGGAGCCCGA GGCCGCCTCC 
ATCTACTGCC GGAAGCTTCG GCTGCACCAG ATGATTGAGC TCAGCAGCAA GGCGGTGGTC 
AATGGGTACA GTGCCAGTGA CACAGTAGGA GCGGGGTTTG CACAGGCTAA GGAACATGTT 
CGACGTAACC GGCAGAGTCG GACCTTTTTG GTGGAGAATG TCATAGGAGA AATCTGGTCT 
GAGCTGGAGG AAGGGGACAA GTACGTGGTT GTTGACAGTG GCGGAGGCAC AGTAGACCTG 
ACCGTCCATC AGATACGGTT ACCAGAGGGG CACCTCAAGG AGCTGTACAA AGCAACAGGT 
GGACCGTACG GATCCCTGGG AGTGGATTAT GAATTTGAAA AACTCCTTTG TAAAATATTT 
GGAGAGGATT TTATCGAACA GTTCAAGATC AAACGCCCGG CAGCCTGGGT TGACCTAATG 
ATTGCCTTTG AGTCTCGCAA AAGAGCTGCT GCACCAGATC GAACCAATCC CCTGAACATC 
ACTCTGCCCT TCTCCTTCAT TGACTACTAC AAGAAGTTCC GGGGCCACAG CGTGGAGCAT 
GCATTGAGGA AGAGCAATGT GGACTTTGTG AAGTGGTCCT CCCAGGGCAT GCTGAGGATG 
AGCCCAGATG CCATGAACGC CCTCTTCAAG CCGACCATCG ACAGCATCAT CGAGCACCTC 
CGGGACCTGT TTCAGAAGCC AGAGGTATCC ACGGTCAAGT TCCTCTTTCT GGTGGGTGGC 
TTTGCAGAGG CGCCCCTTTT GCAGCAGGCA GTGCAGACCG CTTTTGGCGA CAAATGCAGG 
ATCATCATCC CGCAGGATGT GGGCCTCACC ATCCTCAAGG GCGCTGTCCT CTTCGGCCTG 
GACCCTGCCG TTATCAAGGT GCGCCGGTCC CCTCTCACCT ACGGAGTAGG TGTGCTGAAC 
CGCTACGTGG AAGGGAAGCA CCCTCCCGAG AAGCTATTGG TCAAGGACGG CACACGCTGG 
TGCACCGACG TCTTCGACAA GTTCATCTCC GCCGACCAGT CTGTGGCTCT GGGGGAGCTG 
GTCAAGCGTA GCTACACCCC GGCCAAGCCT TCCCAGCTGG TCATCATCAT CAACATCTAC 
AGCTCTGAGC ATGATAACGT CAGCTTCATC ACTGACCCAG GGGTGAAAAA GTGTGGCACG 
CTCCGCTTGG ATCTCACGGG GAGCGGCGGC ACAGCGGTTC CCGCCCGCAG AGAGATCCAG 
ACCATCATGC AGTTCGGGGA CACTGAGATC AAGGCCACGG CCGTCGACAT AACCACCTCA 
AAGAGTGTCA AAGTGGGGAT CGACTTCTTA AATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527442.1
CDS	634..2412
Translation	MRRWEGGDPGVSNQKTPTTILLTPERKFHSFGYAARDFYHDLDPSEAKQWLYLEKFKMKLHTTGDLTMDTDLTAANGKKVKALEIFAYALQYFKEQALKELSDQAGSDFENSDVRWVITVPAIWKQPAKQFMREAAYQAGLASPENSEQLIIALEPEAASIYCRKLRLHQMIELSSKAVVNGYSASDTVGAGFAQAKEHVRRNRQSRTFLVENVIGEIWSELEEGDKYVVVDSGGGTVDLTVHQIRLPEGHLKELYKATGGPYGSLGVDYEFEKLLCKIFGEDFIEQFKIKRPAAWVDLMIAFESRKRAAAPDRTNPLNITLPFSFIDYYKKFRGHSVEHALRKSNVDFVKWSSQGMLRMSPDAMNALFKPTIDSIIEHLRDLFQKPEVSTVKFLFLVGGFAEAPLLQQAVQTAFGDKCRIIIPQDVGLTILKGAVLFGLDPAVIKVRRSPLTYGVGVLNRYVEGKHPPEKLLVKDGTRWCTDVFDKFISADQSVALGELVKRSYTPAKPSQLVIIINIYSSEHDNVSFITDPGVKKCGTLRLDLTGSGGTAVPARREIQTIMQFGDTEIKATAVDITTSKSVKVGIDFLNY

Target ORF information:

RefSeq Version	XM_006527379.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527379.3

ORF Insert Sequence:

ATGCGGAGAT GGGAAGGAGG TGACCCTGGT GTTTCCAACC AGAAGACCCC GACCACCATC 
TTGCTGACAC CAGAGAGGAA GTTCCACAGC TTTGGGTACG CTGCCAGGGA CTTCTACCAT 
GACCTGGACC CCAGTGAGGC CAAGCAATGG CTGTACTTGG AGAAGTTCAA GATGAAACTC 
CACACCACAG GGGACCTCAC CATGGACACA GACTTAACAG CAGCAAATGG CAAGAAGGTC 
AAAGCCCTCG AGATCTTCGC TTACGCCCTG CAGTACTTCA AGGAGCAGGC TCTGAAGGAA 
CTGAGTGACC AGGCAGGGTC GGACTTTGAG AACTCTGACG TCAGATGGGT CATCACAGTG 
CCTGCCATCT GGAAGCAACC AGCCAAGCAG TTCATGAGAG AAGCTGCCTA CCAGGCAGGC 
CTGGCCTCCC CCGAGAACTC GGAGCAGCTC ATCATTGCCC TGGAGCCCGA GGCCGCCTCC 
ATCTACTGCC GGAAGCTTCG GCTGCACCAG ATGATTGAGC TCAGCAGCAA GGCGGTGGTC 
AATGGGTACA GTGCCAGTGA CACAGTAGGA GCGGGGTTTG CACAGGCTAA GGAACATGTT 
CGACGTAACC GGCAGAGTCG GACCTTTTTG GTGGAGAATG TCATAGGAGA AATCTGGTCT 
GAGCTGGAGG AAGGGGACAA GTACGTGGTT GTTGACAGTG GCGGAGGCAC AGTAGACCTG 
ACCGTCCATC AGATACGGTT ACCAGAGGGG CACCTCAAGG AGCTGTACAA AGCAACAGGT 
GGACCGTACG GATCCCTGGG AGTGGATTAT GAATTTGAAA AACTCCTTTG TAAAATATTT 
GGAGAGGATT TTATCGAACA GTTCAAGATC AAACGCCCGG CAGCCTGGGT TGACCTAATG 
ATTGCCTTTG AGTCTCGCAA AAGAGCTGCT GCACCAGATC GAACCAATCC CCTGAACATC 
ACTCTGCCCT TCTCCTTCAT TGACTACTAC AAGAAGTTCC GGGGCCACAG CGTGGAGCAT 
GCATTGAGGA AGAGCAATGT GGACTTTGTG AAGTGGTCCT CCCAGGGCAT GCTGAGGATG 
AGCCCAGATG CCATGAACGC CCTCTTCAAG CCGACCATCG ACAGCATCAT CGAGCACCTC 
CGGGACCTGT TTCAGAAGCC AGAGGTATCC ACGGTCAAGT TCCTCTTTCT GGTGGGTGGC 
TTTGCAGAGG CGCCCCTTTT GCAGCAGGCA GTGCAGACCG CTTTTGGCGA CAAATGCAGG 
ATCATCATCC CGCAGGATGT GGGCCTCACC ATCCTCAAGG GCGCTGTCCT CTTCGGCCTG 
GACCCTGCCG TTATCAAGGT GCGCCGGTCC CCTCTCACCT ACGGAGTAGG TGTGCTGAAC 
CGCTACGTGG AAGGGAAGCA CCCTCCCGAG AAGCTATTGG TCAAGGACGG CACACGCTGG 
TGCACCGACG TCTTCGACAA GTTCATCTCC GCCGACCAGT CTGTGGCTCT GGGGGAGCTG 
GTCAAGCGTA GCTACACCCC GGCCAAGCCT TCCCAGCTGG TCATCATCAT CAACATCTAC 
AGCTCTGAGC ATGATAACGT CAGCTTCATC ACTGACCCAG GGGTGAAAAA GTGTGGCACG 
CTCCGCTTGG ATCTCACGGG GAGCGGCGGC ACAGCGGTTC CCGCCCGCAG AGAGATCCAG 
ACCATCATGC AGTTCGGGGA CACTGAGATC AAGGCCACGG CCGTCGACAT AACCACCTCA 
AAGAGTGTCA AAGTGGGGAT CGACTTCTTA AATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa12a ( XM_011247368.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245670.1 Mus musculus heat shock protein 12A (Hspa12a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu71010
Clone ID Related Accession (Same CDS sequence)	XM_011247368.2 , XM_011247368.3
Accession Version	XM_011247368.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	heat shock 70 kDa protein 12A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGAG AAACAGCCCC CACATCTACA TATTCCTCTC CGGCCAGGAG TCTCGGGGAC 
ACAGGAATAA CACCTCTGTC CCCTTCCCAT ATCTTGAACG ATGCTGACCC CGTCTCTGAG 
CAGCAGACCT TTCTCGTGGT GGTGGCCATC GACTTTGGGA CCACGTCCAG TGGCTATGCC 
TACAGCTTTA CCAAGGAGCC AGAATGTATC CACGTGATGC GGAGATGGGA AGGAGGTGAC 
CCTGGTGTTT CCAACCAGAA GACCCCGACC ACCATCTTGC TGACACCAGA GAGGAAGTTC 
CACAGCTTTG GGTACGCTGC CAGGGACTTC TACCATGACC TGGACCCCAG TGAGGCCAAG 
CAATGGCTGT ACTTGGAGAA GTTCAAGATG AAACTCCACA CCACAGGGGA CCTCACCATG 
GACACAGACT TAACAGCAGC AAATGGCAAG AAGGTCAAAG CCCTCGAGAT CTTCGCTTAC 
GCCCTGCAGT ACTTCAAGGA GCAGGCTCTG AAGGAACTGA GTGACCAGGC AGGGTCGGAC 
TTTGAGAACT CTGACGTCAG ATGGGTCATC ACAGTGCCTG CCATCTGGAA GCAACCAGCC 
AAGCAGTTCA TGAGAGAAGC TGCCTACCAG GCAGGCCTGG CCTCCCCCGA GAACTCGGAG 
CAGCTCATCA TTGCCCTGGA GCCCGAGGCC GCCTCCATCT ACTGCCGGAA GCTTCGGCTG 
CACCAGATGA TTGAGCTCAG CAGCAAGGCG GTGGTCAATG GGTACAGTGC CAGTGACACA 
GTAGGAGCGG GGTTTGCACA GGCTAAGGAA CATGTTCGAC GTAACCGGCA GAGTCGGACC 
TTTTTGGTGG AGAATGTCAT AGGAGAAATC TGGTCTGAGC TGGAGGAAGG GGACAAGTAC 
GTGGTTGTTG ACAGTGGCGG AGGCACAGTA GACCTGACCG TCCATCAGAT ACGGTTACCA 
GAGGGGCACC TCAAGGAGCT GTACAAAGCA ACAGGTGGAC CGTACGGATC CCTGGGAGTG 
GATTATGAAT TTGAAAAACT CCTTTGTAAA ATATTTGGAG AGGATTTTAT CGAACAGTTC 
AAGATCAAAC GCCCGGCAGC CTGGGTTGAC CTAATGATTG CCTTTGAGTC TCGCAAAAGA 
GCTGCTGCAC CAGATCGAAC CAATCCCCTG AACATCACTC TGCCCTTCTC CTTCATTGAC 
TACTACAAGA AGTTCCGGGG CCACAGCGTG GAGCATGCAT TGAGGAAGAG CAATGTGGAC 
TTTGTGAAGT GGTCCTCCCA GGGCATGCTG AGGATGAGCC CAGATGCCAT GAACGCCCTC 
TTCAAGCCGA CCATCGACAG CATCATCGAG CACCTCCGGG ACCTGTTTCA GAAGCCAGAG 
GTATCCACGG TCAAGTTCCT CTTTCTGGTG GGTGGCTTTG CAGAGGCGCC CCTTTTGCAG 
CAGGCAGTGC AGACCGCTTT TGGCGACAAA TGCAGGATCA TCATCCCGCA GGATGTGGGC 
CTCACCATCC TCAAGGGCGC TGTCCTCTTC GGCCTGGACC CTGCCGTTAT CAAGGTGCGC 
CGGTCCCCTC TCACCTACGG AGTAGGTGTG CTGAACCGCT ACGTGGAAGG GAAGCACCCT 
CCCGAGAAGC TATTGGTCAA GGACGGCACA CGCTGGTGCA CCGACGTCTT CGACAAGTTC 
ATCTCCGCCG ACCAGTCTGT GGCTCTGGGG GAGCTGGTCA AGCGTAGCTA CACCCCGGCC 
AAGCCTTCCC AGCTGGTCAT CATCATCAAC ATCTACAGCT CTGAGCATGA TAACGTCAGC 
TTCATCACTG ACCCAGGGGT GAAAAAGTGT GGCACGCTCC GCTTGGATCT CACGGGGAGC 
GGCGGCACAG CGGTTCCCGC CCGCAGAGAG ATCCAGACCA TCATGCAGTT CGGGGACACT 
GAGATCAAGG CCACGGCCGT CGACATAACC ACCTCAAAGA GTGTCAAAGT GGGGATCGAC 
TTCTTAAATT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245670.1
CDS	248..2242
Translation	MTRETAPTSTYSSPARSLGDTGITPLSPSHILNDADPVSEQQTFLVVVAIDFGTTSSGYAYSFTKEPECIHVMRRWEGGDPGVSNQKTPTTILLTPERKFHSFGYAARDFYHDLDPSEAKQWLYLEKFKMKLHTTGDLTMDTDLTAANGKKVKALEIFAYALQYFKEQALKELSDQAGSDFENSDVRWVITVPAIWKQPAKQFMREAAYQAGLASPENSEQLIIALEPEAASIYCRKLRLHQMIELSSKAVVNGYSASDTVGAGFAQAKEHVRRNRQSRTFLVENVIGEIWSELEEGDKYVVVDSGGGTVDLTVHQIRLPEGHLKELYKATGGPYGSLGVDYEFEKLLCKIFGEDFIEQFKIKRPAAWVDLMIAFESRKRAAAPDRTNPLNITLPFSFIDYYKKFRGHSVEHALRKSNVDFVKWSSQGMLRMSPDAMNALFKPTIDSIIEHLRDLFQKPEVSTVKFLFLVGGFAEAPLLQQAVQTAFGDKCRIIIPQDVGLTILKGAVLFGLDPAVIKVRRSPLTYGVGVLNRYVEGKHPPEKLLVKDGTRWCTDVFDKFISADQSVALGELVKRSYTPAKPSQLVIIINIYSSEHDNVSFITDPGVKKCGTLRLDLTGSGGTAVPARREIQTIMQFGDTEIKATAVDITTSKSVKVGIDFLNY

Target ORF information:

RefSeq Version	XM_011247368.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247368.2

ORF Insert Sequence:

ATGACCAGAG AAACAGCCCC CACATCTACA TATTCCTCTC CGGCCAGGAG TCTCGGGGAC 
ACAGGAATAA CACCTCTGTC CCCTTCCCAT ATCTTGAACG ATGCTGACCC CGTCTCTGAG 
CAGCAGACCT TTCTCGTGGT GGTGGCCATC GACTTTGGGA CCACGTCCAG TGGCTATGCC 
TACAGCTTTA CCAAGGAGCC AGAATGTATC CACGTGATGC GGAGATGGGA AGGAGGTGAC 
CCTGGTGTTT CCAACCAGAA GACCCCGACC ACCATCTTGC TGACACCAGA GAGGAAGTTC 
CACAGCTTTG GGTACGCTGC CAGGGACTTC TACCATGACC TGGACCCCAG TGAGGCCAAG 
CAATGGCTGT ACTTGGAGAA GTTCAAGATG AAACTCCACA CCACAGGGGA CCTCACCATG 
GACACAGACT TAACAGCAGC AAATGGCAAG AAGGTCAAAG CCCTCGAGAT CTTCGCTTAC 
GCCCTGCAGT ACTTCAAGGA GCAGGCTCTG AAGGAACTGA GTGACCAGGC AGGGTCGGAC 
TTTGAGAACT CTGACGTCAG ATGGGTCATC ACAGTGCCTG CCATCTGGAA GCAACCAGCC 
AAGCAGTTCA TGAGAGAAGC TGCCTACCAG GCAGGCCTGG CCTCCCCCGA GAACTCGGAG 
CAGCTCATCA TTGCCCTGGA GCCCGAGGCC GCCTCCATCT ACTGCCGGAA GCTTCGGCTG 
CACCAGATGA TTGAGCTCAG CAGCAAGGCG GTGGTCAATG GGTACAGTGC CAGTGACACA 
GTAGGAGCGG GGTTTGCACA GGCTAAGGAA CATGTTCGAC GTAACCGGCA GAGTCGGACC 
TTTTTGGTGG AGAATGTCAT AGGAGAAATC TGGTCTGAGC TGGAGGAAGG GGACAAGTAC 
GTGGTTGTTG ACAGTGGCGG AGGCACAGTA GACCTGACCG TCCATCAGAT ACGGTTACCA 
GAGGGGCACC TCAAGGAGCT GTACAAAGCA ACAGGTGGAC CGTACGGATC CCTGGGAGTG 
GATTATGAAT TTGAAAAACT CCTTTGTAAA ATATTTGGAG AGGATTTTAT CGAACAGTTC 
AAGATCAAAC GCCCGGCAGC CTGGGTTGAC CTAATGATTG CCTTTGAGTC TCGCAAAAGA 
GCTGCTGCAC CAGATCGAAC CAATCCCCTG AACATCACTC TGCCCTTCTC CTTCATTGAC 
TACTACAAGA AGTTCCGGGG CCACAGCGTG GAGCATGCAT TGAGGAAGAG CAATGTGGAC 
TTTGTGAAGT GGTCCTCCCA GGGCATGCTG AGGATGAGCC CAGATGCCAT GAACGCCCTC 
TTCAAGCCGA CCATCGACAG CATCATCGAG CACCTCCGGG ACCTGTTTCA GAAGCCAGAG 
GTATCCACGG TCAAGTTCCT CTTTCTGGTG GGTGGCTTTG CAGAGGCGCC CCTTTTGCAG 
CAGGCAGTGC AGACCGCTTT TGGCGACAAA TGCAGGATCA TCATCCCGCA GGATGTGGGC 
CTCACCATCC TCAAGGGCGC TGTCCTCTTC GGCCTGGACC CTGCCGTTAT CAAGGTGCGC 
CGGTCCCCTC TCACCTACGG AGTAGGTGTG CTGAACCGCT ACGTGGAAGG GAAGCACCCT 
CCCGAGAAGC TATTGGTCAA GGACGGCACA CGCTGGTGCA CCGACGTCTT CGACAAGTTC 
ATCTCCGCCG ACCAGTCTGT GGCTCTGGGG GAGCTGGTCA AGCGTAGCTA CACCCCGGCC 
AAGCCTTCCC AGCTGGTCAT CATCATCAAC ATCTACAGCT CTGAGCATGA TAACGTCAGC 
TTCATCACTG ACCCAGGGGT GAAAAAGTGT GGCACGCTCC GCTTGGATCT CACGGGGAGC 
GGCGGCACAG CGGTTCCCGC CCGCAGAGAG ATCCAGACCA TCATGCAGTT CGGGGACACT 
GAGATCAAGG CCACGGCCGT CGACATAACC ACCTCAAAGA GTGTCAAAGT GGGGATCGAC 
TTCTTAAATT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa12a ( NM_001327998.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001314927.1 Mus musculus heat shock protein 12A (Hspa12a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu55759
Clone ID Related Accession (Same CDS sequence)	NM_001327998.1
Accession Version	NM_001327998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-10
Organism	Mus musculus(house mouse)
Product	heat shock 70 kDa protein 12A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY240671.1, AK087732.1, AK141925.1 and BQ176746.1. On Jun 2, 2016 this sequence version replaced XM_006527378.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK087732.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGAGA GTGTCGGCAT GTACGGGTTC TGTGGCTGCA AAGGAAAGAG CAAGCTGAAG 
TGTGACTCCA GGTGGGAAAT AGCAGCAGCA GCAGCAGAAA CAGCCCCCAC ATCTACATAT 
TCCTCTCCGG CCAGGAGTCT CGGGGACACA GGAATAACAC CTCTGTCCCC TTCCCATATC 
TTGAACGATG CTGACCCCGT CTCTGAGCAG CAGACCTTTC TCGTGGTGGT GGCCATCGAC 
TTTGGGACCA CGTCCAGTGG CTATGCCTAC AGCTTTACCA AGGAGCCAGA ATGTATCCAC 
GTGATGCGGA GATGGGAAGG AGGTGACCCT GGTGTTTCCA ACCAGAAGAC CCCGACCACC 
ATCTTGCTGA CACCAGAGAG GAAGTTCCAC AGCTTTGGGT ACGCTGCCAG GGACTTCTAC 
CATGACCTGG ACCCCAGTGA GGCCAAGCAA TGGCTGTACT TGGAGAAGTT CAAGATGAAA 
CTCCACACCA CAGGGGACCT CACCATGGAC ACAGACTTAA CAGCAGCAAA TGGCAAGAAG 
GTCAAAGCCC TCGAGATCTT CGCTTACGCC CTGCAGTACT TCAAGGAGCA GGCTCTGAAG 
GAACTGAGTG ACCAGGCAGG GTCGGACTTT GAGAACTCTG ACGTCAGATG GGTCATCACA 
GTGCCTGCCA TCTGGAAGCA ACCAGCCAAG CAGTTCATGA GAGAAGCTGC CTACCAGGCA 
GGCCTGGCCT CCCCCGAGAA CTCGGAGCAG CTCATCATTG CCCTGGAGCC CGAGGCCGCC 
TCCATCTACT GCCGGAAGCT TCGGCTGCAC CAGATGATTG AGCTCAGCAG CAAGGCGGTG 
GTCAATGGGT ACAGTGCCAG TGACACAGTA GGAGCGGGGT TTGCACAGGC TAAGGAACAT 
GTTCGACGTA ACCGGCAGAG TCGGACCTTT TTGGTGGAGA ATGTCATAGG AGAAATCTGG 
TCTGAGCTGG AGGAAGGGGA CAAGTACGTG GTTGTTGACA GTGGCGGAGG CACAGTAGAC 
CTGACCGTCC ATCAGATACG GTTACCAGAG GGGCACCTCA AGGAGCTGTA CAAAGCAACA 
GGTGGACCGT ACGGATCCCT GGGAGTGGAT TATGAATTTG AAAAACTCCT TTGTAAAATA 
TTTGGAGAGG ATTTTATCGA ACAGTTCAAG ATCAAACGCC CGGCAGCCTG GGTTGACCTA 
ATGATTGCCT TTGAGTCTCG CAAAAGAGCT GCTGCACCAG ATCGAACCAA TCCCCTGAAC 
ATCACTCTGC CCTTCTCCTT CATTGACTAC TACAAGAAGT TCCGGGGCCA CAGCGTGGAG 
CATGCATTGA GGAAGAGCAA TGTGGACTTT GTGAAGTGGT CCTCCCAGGG CATGCTGAGG 
ATGAGCCCAG ATGCCATGAA CGCCCTCTTC AAGCCGACCA TCGACAGCAT CATCGAGCAC 
CTCCGGGACC TGTTTCAGAA GCCAGAGGTA TCCACGGTCA AGTTCCTCTT TCTGGTGGGT 
GGCTTTGCAG AGGCGCCCCT TTTGCAGCAG GCAGTGCAGA CCGCTTTTGG CGACAAATGC 
AGGATCATCA TCCCGCAGGA TGTGGGCCTC ACCATCCTCA AGGGCGCTGT CCTCTTCGGC 
CTGGACCCTG CCGTTATCAA GGTGCGCCGG TCCCCTCTCA CCTACGGAGT AGGTGTGCTG 
AACCGCTACG TGGAAGGGAA GCACCCTCCC GAGAAGCTAT TGGTCAAGGA CGGCACACGC 
TGGTGCACCG ACGTCTTCGA CAAGTTCATC TCCGCCGACC AGTCTGTGGC TCTGGGGGAG 
CTGGTCAAGC GTAGCTACAC CCCGGCCAAG CCTTCCCAGC TGGTCATCAT CATCAACATC 
TACAGCTCTG AGCATGATAA CGTCAGCTTC ATCACTGACC CAGGGGTGAA AAAGTGTGGC 
ACGCTCCGCT TGGATCTCAC GGGGAGCGGC GGCACAGCGG TTCCCGCCCG CAGAGAGATC 
CAGACCATCA TGCAGTTCGG GGACACTGAG ATCAAGGCCA CGGCCGTCGA CATAACCACC 
TCAAAGAGTG TCAAAGTGGG GATCGACTTC TTAAATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314927.1
CDS	215..2296
Translation	MMESVGMYGFCGCKGKSKLKCDSRWEIAAAAAETAPTSTYSSPARSLGDTGITPLSPSHILNDADPVSEQQTFLVVVAIDFGTTSSGYAYSFTKEPECIHVMRRWEGGDPGVSNQKTPTTILLTPERKFHSFGYAARDFYHDLDPSEAKQWLYLEKFKMKLHTTGDLTMDTDLTAANGKKVKALEIFAYALQYFKEQALKELSDQAGSDFENSDVRWVITVPAIWKQPAKQFMREAAYQAGLASPENSEQLIIALEPEAASIYCRKLRLHQMIELSSKAVVNGYSASDTVGAGFAQAKEHVRRNRQSRTFLVENVIGEIWSELEEGDKYVVVDSGGGTVDLTVHQIRLPEGHLKELYKATGGPYGSLGVDYEFEKLLCKIFGEDFIEQFKIKRPAAWVDLMIAFESRKRAAAPDRTNPLNITLPFSFIDYYKKFRGHSVEHALRKSNVDFVKWSSQGMLRMSPDAMNALFKPTIDSIIEHLRDLFQKPEVSTVKFLFLVGGFAEAPLLQQAVQTAFGDKCRIIIPQDVGLTILKGAVLFGLDPAVIKVRRSPLTYGVGVLNRYVEGKHPPEKLLVKDGTRWCTDVFDKFISADQSVALGELVKRSYTPAKPSQLVIIINIYSSEHDNVSFITDPGVKKCGTLRLDLTGSGGTAVPARREIQTIMQFGDTEIKATAVDITTSKSVKVGIDFLNY

Target ORF information:

RefSeq Version	NM_001327998.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 12A (Hspa12a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001327998.1

ORF Insert Sequence:

ATGATGGAGA GTGTCGGCAT GTACGGGTTC TGTGGCTGCA AAGGAAAGAG CAAGCTGAAG 
TGTGACTCCA GGTGGGAAAT AGCAGCAGCA GCAGCAGAAA CAGCCCCCAC ATCTACATAT 
TCCTCTCCGG CCAGGAGTCT CGGGGACACA GGAATAACAC CTCTGTCCCC TTCCCATATC 
TTGAACGATG CTGACCCCGT CTCTGAGCAG CAGACCTTTC TCGTGGTGGT GGCCATCGAC 
TTTGGGACCA CGTCCAGTGG CTATGCCTAC AGCTTTACCA AGGAGCCAGA ATGTATCCAC 
GTGATGCGGA GATGGGAAGG AGGTGACCCT GGTGTTTCCA ACCAGAAGAC CCCGACCACC 
ATCTTGCTGA CACCAGAGAG GAAGTTCCAC AGCTTTGGGT ACGCTGCCAG GGACTTCTAC 
CATGACCTGG ACCCCAGTGA GGCCAAGCAA TGGCTGTACT TGGAGAAGTT CAAGATGAAA 
CTCCACACCA CAGGGGACCT CACCATGGAC ACAGACTTAA CAGCAGCAAA TGGCAAGAAG 
GTCAAAGCCC TCGAGATCTT CGCTTACGCC CTGCAGTACT TCAAGGAGCA GGCTCTGAAG 
GAACTGAGTG ACCAGGCAGG GTCGGACTTT GAGAACTCTG ACGTCAGATG GGTCATCACA 
GTGCCTGCCA TCTGGAAGCA ACCAGCCAAG CAGTTCATGA GAGAAGCTGC CTACCAGGCA 
GGCCTGGCCT CCCCCGAGAA CTCGGAGCAG CTCATCATTG CCCTGGAGCC CGAGGCCGCC 
TCCATCTACT GCCGGAAGCT TCGGCTGCAC CAGATGATTG AGCTCAGCAG CAAGGCGGTG 
GTCAATGGGT ACAGTGCCAG TGACACAGTA GGAGCGGGGT TTGCACAGGC TAAGGAACAT 
GTTCGACGTA ACCGGCAGAG TCGGACCTTT TTGGTGGAGA ATGTCATAGG AGAAATCTGG 
TCTGAGCTGG AGGAAGGGGA CAAGTACGTG GTTGTTGACA GTGGCGGAGG CACAGTAGAC 
CTGACCGTCC ATCAGATACG GTTACCAGAG GGGCACCTCA AGGAGCTGTA CAAAGCAACA 
GGTGGACCGT ACGGATCCCT GGGAGTGGAT TATGAATTTG AAAAACTCCT TTGTAAAATA 
TTTGGAGAGG ATTTTATCGA ACAGTTCAAG ATCAAACGCC CGGCAGCCTG GGTTGACCTA 
ATGATTGCCT TTGAGTCTCG CAAAAGAGCT GCTGCACCAG ATCGAACCAA TCCCCTGAAC 
ATCACTCTGC CCTTCTCCTT CATTGACTAC TACAAGAAGT TCCGGGGCCA CAGCGTGGAG 
CATGCATTGA GGAAGAGCAA TGTGGACTTT GTGAAGTGGT CCTCCCAGGG CATGCTGAGG 
ATGAGCCCAG ATGCCATGAA CGCCCTCTTC AAGCCGACCA TCGACAGCAT CATCGAGCAC 
CTCCGGGACC TGTTTCAGAA GCCAGAGGTA TCCACGGTCA AGTTCCTCTT TCTGGTGGGT 
GGCTTTGCAG AGGCGCCCCT TTTGCAGCAG GCAGTGCAGA CCGCTTTTGG CGACAAATGC 
AGGATCATCA TCCCGCAGGA TGTGGGCCTC ACCATCCTCA AGGGCGCTGT CCTCTTCGGC 
CTGGACCCTG CCGTTATCAA GGTGCGCCGG TCCCCTCTCA CCTACGGAGT AGGTGTGCTG 
AACCGCTACG TGGAAGGGAA GCACCCTCCC GAGAAGCTAT TGGTCAAGGA CGGCACACGC 
TGGTGCACCG ACGTCTTCGA CAAGTTCATC TCCGCCGACC AGTCTGTGGC TCTGGGGGAG 
CTGGTCAAGC GTAGCTACAC CCCGGCCAAG CCTTCCCAGC TGGTCATCAT CATCAACATC 
TACAGCTCTG AGCATGATAA CGTCAGCTTC ATCACTGACC CAGGGGTGAA AAAGTGTGGC 
ACGCTCCGCT TGGATCTCAC GGGGAGCGGC GGCACAGCGG TTCCCGCCCG CAGAGAGATC 
CAGACCATCA TGCAGTTCGG GGACACTGAG ATCAAGGCCA CGGCCGTCGA CATAACCACC 
TCAAAGAGTG TCAAAGTGGG GATCGACTTC TTAAATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa12a ( XM_006527377.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527440.1 Mus musculus heat shock protein 12A (Hspa12a), transcript variant X1, mRNA.

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CloneID	OMu55758
Clone ID Related Accession (Same CDS sequence)	XM_006527377.2
Accession Version	XM_006527377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	heat shock 70 kDa protein 12A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006527377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAGAG TTTCCCTGTG TGTTTTATGT CCTCTCTGCA CATTGGAGTC TTGGTTCTGC 
TGCAGATGCA TCACAGTTTT TCAAGACCGG GAGCTTAGTA CAGAAACAGC CCCCACATCT 
ACATATTCCT CTCCGGCCAG GAGTCTCGGG GACACAGGAA TAACACCTCT GTCCCCTTCC 
CATATCTTGA ACGATGCTGA CCCCGTCTCT GAGCAGCAGA CCTTTCTCGT GGTGGTGGCC 
ATCGACTTTG GGACCACGTC CAGTGGCTAT GCCTACAGCT TTACCAAGGA GCCAGAATGT 
ATCCACGTGA TGCGGAGATG GGAAGGAGGT GACCCTGGTG TTTCCAACCA GAAGACCCCG 
ACCACCATCT TGCTGACACC AGAGAGGAAG TTCCACAGCT TTGGGTACGC TGCCAGGGAC 
TTCTACCATG ACCTGGACCC CAGTGAGGCC AAGCAATGGC TGTACTTGGA GAAGTTCAAG 
ATGAAACTCC ACACCACAGG GGACCTCACC ATGGACACAG ACTTAACAGC AGCAAATGGC 
AAGAAGGTCA AAGCCCTCGA GATCTTCGCT TACGCCCTGC AGTACTTCAA GGAGCAGGCT 
CTGAAGGAAC TGAGTGACCA GGCAGGGTCG GACTTTGAGA ACTCTGACGT CAGATGGGTC 
ATCACAGTGC CTGCCATCTG GAAGCAACCA GCCAAGCAGT TCATGAGAGA AGCTGCCTAC 
CAGGCAGGCC TGGCCTCCCC CGAGAACTCG GAGCAGCTCA TCATTGCCCT GGAGCCCGAG 
GCCGCCTCCA TCTACTGCCG GAAGCTTCGG CTGCACCAGA TGATTGAGCT CAGCAGCAAG 
GCGGTGGTCA ATGGGTACAG TGCCAGTGAC ACAGTAGGAG CGGGGTTTGC ACAGGCTAAG 
GAACATGTTC GACGTAACCG GCAGAGTCGG ACCTTTTTGG TGGAGAATGT CATAGGAGAA 
ATCTGGTCTG AGCTGGAGGA AGGGGACAAG TACGTGGTTG TTGACAGTGG CGGAGGCACA 
GTAGACCTGA CCGTCCATCA GATACGGTTA CCAGAGGGGC ACCTCAAGGA GCTGTACAAA 
GCAACAGGTG GACCGTACGG ATCCCTGGGA GTGGATTATG AATTTGAAAA ACTCCTTTGT 
AAAATATTTG GAGAGGATTT TATCGAACAG TTCAAGATCA AACGCCCGGC AGCCTGGGTT 
GACCTAATGA TTGCCTTTGA GTCTCGCAAA AGAGCTGCTG CACCAGATCG AACCAATCCC 
CTGAACATCA CTCTGCCCTT CTCCTTCATT GACTACTACA AGAAGTTCCG GGGCCACAGC 
GTGGAGCATG CATTGAGGAA GAGCAATGTG GACTTTGTGA AGTGGTCCTC CCAGGGCATG 
CTGAGGATGA GCCCAGATGC CATGAACGCC CTCTTCAAGC CGACCATCGA CAGCATCATC 
GAGCACCTCC GGGACCTGTT TCAGAAGCCA GAGGTATCCA CGGTCAAGTT CCTCTTTCTG 
GTGGGTGGCT TTGCAGAGGC GCCCCTTTTG CAGCAGGCAG TGCAGACCGC TTTTGGCGAC 
AAATGCAGGA TCATCATCCC GCAGGATGTG GGCCTCACCA TCCTCAAGGG CGCTGTCCTC 
TTCGGCCTGG ACCCTGCCGT TATCAAGGTG CGCCGGTCCC CTCTCACCTA CGGAGTAGGT 
GTGCTGAACC GCTACGTGGA AGGGAAGCAC CCTCCCGAGA AGCTATTGGT CAAGGACGGC 
ACACGCTGGT GCACCGACGT CTTCGACAAG TTCATCTCCG CCGACCAGTC TGTGGCTCTG 
GGGGAGCTGG TCAAGCGTAG CTACACCCCG GCCAAGCCTT CCCAGCTGGT CATCATCATC 
AACATCTACA GCTCTGAGCA TGATAACGTC AGCTTCATCA CTGACCCAGG GGTGAAAAAG 
TGTGGCACGC TCCGCTTGGA TCTCACGGGG AGCGGCGGCA CAGCGGTTCC CGCCCGCAGA 
GAGATCCAGA CCATCATGCA GTTCGGGGAC ACTGAGATCA AGGCCACGGC CGTCGACATA 
ACCACCTCAA AGAGTGTCAA AGTGGGGATC GACTTCTTAA ATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527440.1
CDS	16..2103
Translation	MPRVSLCVLCPLCTLESWFCCRCITVFQDRELSTETAPTSTYSSPARSLGDTGITPLSPSHILNDADPVSEQQTFLVVVAIDFGTTSSGYAYSFTKEPECIHVMRRWEGGDPGVSNQKTPTTILLTPERKFHSFGYAARDFYHDLDPSEAKQWLYLEKFKMKLHTTGDLTMDTDLTAANGKKVKALEIFAYALQYFKEQALKELSDQAGSDFENSDVRWVITVPAIWKQPAKQFMREAAYQAGLASPENSEQLIIALEPEAASIYCRKLRLHQMIELSSKAVVNGYSASDTVGAGFAQAKEHVRRNRQSRTFLVENVIGEIWSELEEGDKYVVVDSGGGTVDLTVHQIRLPEGHLKELYKATGGPYGSLGVDYEFEKLLCKIFGEDFIEQFKIKRPAAWVDLMIAFESRKRAAAPDRTNPLNITLPFSFIDYYKKFRGHSVEHALRKSNVDFVKWSSQGMLRMSPDAMNALFKPTIDSIIEHLRDLFQKPEVSTVKFLFLVGGFAEAPLLQQAVQTAFGDKCRIIIPQDVGLTILKGAVLFGLDPAVIKVRRSPLTYGVGVLNRYVEGKHPPEKLLVKDGTRWCTDVFDKFISADQSVALGELVKRSYTPAKPSQLVIIINIYSSEHDNVSFITDPGVKKCGTLRLDLTGSGGTAVPARREIQTIMQFGDTEIKATAVDITTSKSVKVGIDFLNY

Target ORF information:

RefSeq Version	XM_006527377.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527377.2

ORF Insert Sequence:

ATGCCCAGAG TTTCCCTGTG TGTTTTATGT CCTCTCTGCA CATTGGAGTC TTGGTTCTGC 
TGCAGATGCA TCACAGTTTT TCAAGACCGG GAGCTTAGTA CAGAAACAGC CCCCACATCT 
ACATATTCCT CTCCGGCCAG GAGTCTCGGG GACACAGGAA TAACACCTCT GTCCCCTTCC 
CATATCTTGA ACGATGCTGA CCCCGTCTCT GAGCAGCAGA CCTTTCTCGT GGTGGTGGCC 
ATCGACTTTG GGACCACGTC CAGTGGCTAT GCCTACAGCT TTACCAAGGA GCCAGAATGT 
ATCCACGTGA TGCGGAGATG GGAAGGAGGT GACCCTGGTG TTTCCAACCA GAAGACCCCG 
ACCACCATCT TGCTGACACC AGAGAGGAAG TTCCACAGCT TTGGGTACGC TGCCAGGGAC 
TTCTACCATG ACCTGGACCC CAGTGAGGCC AAGCAATGGC TGTACTTGGA GAAGTTCAAG 
ATGAAACTCC ACACCACAGG GGACCTCACC ATGGACACAG ACTTAACAGC AGCAAATGGC 
AAGAAGGTCA AAGCCCTCGA GATCTTCGCT TACGCCCTGC AGTACTTCAA GGAGCAGGCT 
CTGAAGGAAC TGAGTGACCA GGCAGGGTCG GACTTTGAGA ACTCTGACGT CAGATGGGTC 
ATCACAGTGC CTGCCATCTG GAAGCAACCA GCCAAGCAGT TCATGAGAGA AGCTGCCTAC 
CAGGCAGGCC TGGCCTCCCC CGAGAACTCG GAGCAGCTCA TCATTGCCCT GGAGCCCGAG 
GCCGCCTCCA TCTACTGCCG GAAGCTTCGG CTGCACCAGA TGATTGAGCT CAGCAGCAAG 
GCGGTGGTCA ATGGGTACAG TGCCAGTGAC ACAGTAGGAG CGGGGTTTGC ACAGGCTAAG 
GAACATGTTC GACGTAACCG GCAGAGTCGG ACCTTTTTGG TGGAGAATGT CATAGGAGAA 
ATCTGGTCTG AGCTGGAGGA AGGGGACAAG TACGTGGTTG TTGACAGTGG CGGAGGCACA 
GTAGACCTGA CCGTCCATCA GATACGGTTA CCAGAGGGGC ACCTCAAGGA GCTGTACAAA 
GCAACAGGTG GACCGTACGG ATCCCTGGGA GTGGATTATG AATTTGAAAA ACTCCTTTGT 
AAAATATTTG GAGAGGATTT TATCGAACAG TTCAAGATCA AACGCCCGGC AGCCTGGGTT 
GACCTAATGA TTGCCTTTGA GTCTCGCAAA AGAGCTGCTG CACCAGATCG AACCAATCCC 
CTGAACATCA CTCTGCCCTT CTCCTTCATT GACTACTACA AGAAGTTCCG GGGCCACAGC 
GTGGAGCATG CATTGAGGAA GAGCAATGTG GACTTTGTGA AGTGGTCCTC CCAGGGCATG 
CTGAGGATGA GCCCAGATGC CATGAACGCC CTCTTCAAGC CGACCATCGA CAGCATCATC 
GAGCACCTCC GGGACCTGTT TCAGAAGCCA GAGGTATCCA CGGTCAAGTT CCTCTTTCTG 
GTGGGTGGCT TTGCAGAGGC GCCCCTTTTG CAGCAGGCAG TGCAGACCGC TTTTGGCGAC 
AAATGCAGGA TCATCATCCC GCAGGATGTG GGCCTCACCA TCCTCAAGGG CGCTGTCCTC 
TTCGGCCTGG ACCCTGCCGT TATCAAGGTG CGCCGGTCCC CTCTCACCTA CGGAGTAGGT 
GTGCTGAACC GCTACGTGGA AGGGAAGCAC CCTCCCGAGA AGCTATTGGT CAAGGACGGC 
ACACGCTGGT GCACCGACGT CTTCGACAAG TTCATCTCCG CCGACCAGTC TGTGGCTCTG 
GGGGAGCTGG TCAAGCGTAG CTACACCCCG GCCAAGCCTT CCCAGCTGGT CATCATCATC 
AACATCTACA GCTCTGAGCA TGATAACGTC AGCTTCATCA CTGACCCAGG GGTGAAAAAG 
TGTGGCACGC TCCGCTTGGA TCTCACGGGG AGCGGCGGCA CAGCGGTTCC CGCCCGCAGA 
GAGATCCAGA CCATCATGCA GTTCGGGGAC ACTGAGATCA AGGCCACGGC CGTCGACATA 
ACCACCTCAA AGAGTGTCAA AGTGGGGATC GACTTCTTAA ATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa12a ( NM_175199.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780408.1 Mus musculus heat shock protein 12A (Hspa12a), transcript variant 2, mRNA.

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CloneID	OMu04998
Clone ID Related Accession (Same CDS sequence)	NM_175199.3
Accession Version	NM_175199.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-10
Organism	Mus musculus(house mouse)
Product	heat shock 70 kDa protein 12A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK141925.1. On Apr 6, 2007 this sequence version replaced NM_175199.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and uses an alternate start codon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK141925.1, SRR1660815.75441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACA AGGAAGCTGG CGGTGGAGAC GCCGGGCCCC GAGAAACAGC CCCCACATCT 
ACATATTCCT CTCCGGCCAG GAGTCTCGGG GACACAGGAA TAACACCTCT GTCCCCTTCC 
CATATCTTGA ACGATGCTGA CCCCGTCTCT GAGCAGCAGA CCTTTCTCGT GGTGGTGGCC 
ATCGACTTTG GGACCACGTC CAGTGGCTAT GCCTACAGCT TTACCAAGGA GCCAGAATGT 
ATCCACGTGA TGCGGAGATG GGAAGGAGGT GACCCTGGTG TTTCCAACCA GAAGACCCCG 
ACCACCATCT TGCTGACACC AGAGAGGAAG TTCCACAGCT TTGGGTACGC TGCCAGGGAC 
TTCTACCATG ACCTGGACCC CAGTGAGGCC AAGCAATGGC TGTACTTGGA GAAGTTCAAG 
ATGAAACTCC ACACCACAGG GGACCTCACC ATGGACACAG ACTTAACAGC AGCAAATGGC 
AAGAAGGTCA AAGCCCTCGA GATCTTCGCT TACGCCCTGC AGTACTTCAA GGAGCAGGCT 
CTGAAGGAAC TGAGTGACCA GGCAGGGTCG GACTTTGAGA ACTCTGACGT CAGATGGGTC 
ATCACAGTGC CTGCCATCTG GAAGCAACCA GCCAAGCAGT TCATGAGAGA AGCTGCCTAC 
CAGGCAGGCC TGGCCTCCCC CGAGAACTCG GAGCAGCTCA TCATTGCCCT GGAGCCCGAG 
GCCGCCTCCA TCTACTGCCG GAAGCTTCGG CTGCACCAGA TGATTGAGCT CAGCAGCAAG 
GCGGTGGTCA ATGGGTACAG TGCCAGTGAC ACAGTAGGAG CGGGGTTTGC ACAGGCTAAG 
GAACATGTTC GACGTAACCG GCAGAGTCGG ACCTTTTTGG TGGAGAATGT CATAGGAGAA 
ATCTGGTCTG AGCTGGAGGA AGGGGACAAG TACGTGGTTG TTGACAGTGG CGGAGGCACA 
GTAGACCTGA CCGTCCATCA GATACGGTTA CCAGAGGGGC ACCTCAAGGA GCTGTACAAA 
GCAACAGGTG GACCGTACGG ATCCCTGGGA GTGGATTATG AATTTGAAAA ACTCCTTTGT 
AAAATATTTG GAGAGGATTT TATCGAACAG TTCAAGATCA AACGCCCGGC AGCCTGGGTT 
GACCTAATGA TTGCCTTTGA GTCTCGCAAA AGAGCTGCTG CACCAGATCG AACCAATCCC 
CTGAACATCA CTCTGCCCTT CTCCTTCATT GACTACTACA AGAAGTTCCG GGGCCACAGC 
GTGGAGCATG CATTGAGGAA GAGCAATGTG GACTTTGTGA AGTGGTCCTC CCAGGGCATG 
CTGAGGATGA GCCCAGATGC CATGAACGCC CTCTTCAAGC CGACCATCGA CAGCATCATC 
GAGCACCTCC GGGACCTGTT TCAGAAGCCA GAGGTATCCA CGGTCAAGTT CCTCTTTCTG 
GTGGGTGGCT TTGCAGAGGC GCCCCTTTTG CAGCAGGCAG TGCAGACCGC TTTTGGCGAC 
AAATGCAGGA TCATCATCCC GCAGGATGTG GGCCTCACCA TCCTCAAGGG CGCTGTCCTC 
TTCGGCCTGG ACCCTGCCGT TATCAAGGTG CGCCGGTCCC CTCTCACCTA CGGAGTAGGT 
GTGCTGAACC GCTACGTGGA AGGGAAGCAC CCTCCCGAGA AGCTATTGGT CAAGGACGGC 
ACACGCTGGT GCACCGACGT CTTCGACAAG TTCATCTCCG CCGACCAGTC TGTGGCTCTG 
GGGGAGCTGG TCAAGCGTAG CTACACCCCG GCCAAGCCTT CCCAGCTGGT CATCATCATC 
AACATCTACA GCTCTGAGCA TGATAACGTC AGCTTCATCA CTGACCCAGG GGTGAAAAAG 
TGTGGCACGC TCCGCTTGGA TCTCACGGGG AGCGGCGGCA CAGCGGTTCC CGCCCGCAGA 
GAGATCCAGA CCATCATGCA GTTCGGGGAC ACTGAGATCA AGGCCACGGC CGTCGACATA 
ACCACCTCAA AGAGTGTCAA AGTGGGGATC GACTTCTTAA ATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780408.1
CDS	96..2123
Translation	MADKEAGGGDAGPRETAPTSTYSSPARSLGDTGITPLSPSHILNDADPVSEQQTFLVVVAIDFGTTSSGYAYSFTKEPECIHVMRRWEGGDPGVSNQKTPTTILLTPERKFHSFGYAARDFYHDLDPSEAKQWLYLEKFKMKLHTTGDLTMDTDLTAANGKKVKALEIFAYALQYFKEQALKELSDQAGSDFENSDVRWVITVPAIWKQPAKQFMREAAYQAGLASPENSEQLIIALEPEAASIYCRKLRLHQMIELSSKAVVNGYSASDTVGAGFAQAKEHVRRNRQSRTFLVENVIGEIWSELEEGDKYVVVDSGGGTVDLTVHQIRLPEGHLKELYKATGGPYGSLGVDYEFEKLLCKIFGEDFIEQFKIKRPAAWVDLMIAFESRKRAAAPDRTNPLNITLPFSFIDYYKKFRGHSVEHALRKSNVDFVKWSSQGMLRMSPDAMNALFKPTIDSIIEHLRDLFQKPEVSTVKFLFLVGGFAEAPLLQQAVQTAFGDKCRIIIPQDVGLTILKGAVLFGLDPAVIKVRRSPLTYGVGVLNRYVEGKHPPEKLLVKDGTRWCTDVFDKFISADQSVALGELVKRSYTPAKPSQLVIIINIYSSEHDNVSFITDPGVKKCGTLRLDLTGSGGTAVPARREIQTIMQFGDTEIKATAVDITTSKSVKVGIDFLNY

Target ORF information:

RefSeq Version	NM_175199.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 12A (Hspa12a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175199.3

ORF Insert Sequence:

ATGGCGGACA AGGAAGCTGG CGGTGGAGAC GCCGGGCCCC GAGAAACAGC CCCCACATCT 
ACATATTCCT CTCCGGCCAG GAGTCTCGGG GACACAGGAA TAACACCTCT GTCCCCTTCC 
CATATCTTGA ACGATGCTGA CCCCGTCTCT GAGCAGCAGA CCTTTCTCGT GGTGGTGGCC 
ATCGACTTTG GGACCACGTC CAGTGGCTAT GCCTACAGCT TTACCAAGGA GCCAGAATGT 
ATCCACGTGA TGCGGAGATG GGAAGGAGGT GACCCTGGTG TTTCCAACCA GAAGACCCCG 
ACCACCATCT TGCTGACACC AGAGAGGAAG TTCCACAGCT TTGGGTACGC TGCCAGGGAC 
TTCTACCATG ACCTGGACCC CAGTGAGGCC AAGCAATGGC TGTACTTGGA GAAGTTCAAG 
ATGAAACTCC ACACCACAGG GGACCTCACC ATGGACACAG ACTTAACAGC AGCAAATGGC 
AAGAAGGTCA AAGCCCTCGA GATCTTCGCT TACGCCCTGC AGTACTTCAA GGAGCAGGCT 
CTGAAGGAAC TGAGTGACCA GGCAGGGTCG GACTTTGAGA ACTCTGACGT CAGATGGGTC 
ATCACAGTGC CTGCCATCTG GAAGCAACCA GCCAAGCAGT TCATGAGAGA AGCTGCCTAC 
CAGGCAGGCC TGGCCTCCCC CGAGAACTCG GAGCAGCTCA TCATTGCCCT GGAGCCCGAG 
GCCGCCTCCA TCTACTGCCG GAAGCTTCGG CTGCACCAGA TGATTGAGCT CAGCAGCAAG 
GCGGTGGTCA ATGGGTACAG TGCCAGTGAC ACAGTAGGAG CGGGGTTTGC ACAGGCTAAG 
GAACATGTTC GACGTAACCG GCAGAGTCGG ACCTTTTTGG TGGAGAATGT CATAGGAGAA 
ATCTGGTCTG AGCTGGAGGA AGGGGACAAG TACGTGGTTG TTGACAGTGG CGGAGGCACA 
GTAGACCTGA CCGTCCATCA GATACGGTTA CCAGAGGGGC ACCTCAAGGA GCTGTACAAA 
GCAACAGGTG GACCGTACGG ATCCCTGGGA GTGGATTATG AATTTGAAAA ACTCCTTTGT 
AAAATATTTG GAGAGGATTT TATCGAACAG TTCAAGATCA AACGCCCGGC AGCCTGGGTT 
GACCTAATGA TTGCCTTTGA GTCTCGCAAA AGAGCTGCTG CACCAGATCG AACCAATCCC 
CTGAACATCA CTCTGCCCTT CTCCTTCATT GACTACTACA AGAAGTTCCG GGGCCACAGC 
GTGGAGCATG CATTGAGGAA GAGCAATGTG GACTTTGTGA AGTGGTCCTC CCAGGGCATG 
CTGAGGATGA GCCCAGATGC CATGAACGCC CTCTTCAAGC CGACCATCGA CAGCATCATC 
GAGCACCTCC GGGACCTGTT TCAGAAGCCA GAGGTATCCA CGGTCAAGTT CCTCTTTCTG 
GTGGGTGGCT TTGCAGAGGC GCCCCTTTTG CAGCAGGCAG TGCAGACCGC TTTTGGCGAC 
AAATGCAGGA TCATCATCCC GCAGGATGTG GGCCTCACCA TCCTCAAGGG CGCTGTCCTC 
TTCGGCCTGG ACCCTGCCGT TATCAAGGTG CGCCGGTCCC CTCTCACCTA CGGAGTAGGT 
GTGCTGAACC GCTACGTGGA AGGGAAGCAC CCTCCCGAGA AGCTATTGGT CAAGGACGGC 
ACACGCTGGT GCACCGACGT CTTCGACAAG TTCATCTCCG CCGACCAGTC TGTGGCTCTG 
GGGGAGCTGG TCAAGCGTAG CTACACCCCG GCCAAGCCTT CCCAGCTGGT CATCATCATC 
AACATCTACA GCTCTGAGCA TGATAACGTC AGCTTCATCA CTGACCCAGG GGTGAAAAAG 
TGTGGCACGC TCCGCTTGGA TCTCACGGGG AGCGGCGGCA CAGCGGTTCC CGCCCGCAGA 
GAGATCCAGA CCATCATGCA GTTCGGGGAC ACTGAGATCA AGGCCACGGC CGTCGACATA 
ACCACCTCAA AGAGTGTCAA AGTGGGGATC GACTTCTTAA ATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa12a ( XM_006527379.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527442.1 Mus musculus heat shock protein 12A (Hspa12a), transcript variant X3, mRNA.

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CloneID	OMu55760
Clone ID Related Accession (Same CDS sequence)	XM_006527379.3 , XM_006527379.4
Accession Version	XM_006527379.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	heat shock 70 kDa protein 12A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527379.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGAT GGGAAGGAGG TGACCCTGGT GTTTCCAACC AGAAGACCCC GACCACCATC 
TTGCTGACAC CAGAGAGGAA GTTCCACAGC TTTGGGTACG CTGCCAGGGA CTTCTACCAT 
GACCTGGACC CCAGTGAGGC CAAGCAATGG CTGTACTTGG AGAAGTTCAA GATGAAACTC 
CACACCACAG GGGACCTCAC CATGGACACA GACTTAACAG CAGCAAATGG CAAGAAGGTC 
AAAGCCCTCG AGATCTTCGC TTACGCCCTG CAGTACTTCA AGGAGCAGGC TCTGAAGGAA 
CTGAGTGACC AGGCAGGGTC GGACTTTGAG AACTCTGACG TCAGATGGGT CATCACAGTG 
CCTGCCATCT GGAAGCAACC AGCCAAGCAG TTCATGAGAG AAGCTGCCTA CCAGGCAGGC 
CTGGCCTCCC CCGAGAACTC GGAGCAGCTC ATCATTGCCC TGGAGCCCGA GGCCGCCTCC 
ATCTACTGCC GGAAGCTTCG GCTGCACCAG ATGATTGAGC TCAGCAGCAA GGCGGTGGTC 
AATGGGTACA GTGCCAGTGA CACAGTAGGA GCGGGGTTTG CACAGGCTAA GGAACATGTT 
CGACGTAACC GGCAGAGTCG GACCTTTTTG GTGGAGAATG TCATAGGAGA AATCTGGTCT 
GAGCTGGAGG AAGGGGACAA GTACGTGGTT GTTGACAGTG GCGGAGGCAC AGTAGACCTG 
ACCGTCCATC AGATACGGTT ACCAGAGGGG CACCTCAAGG AGCTGTACAA AGCAACAGGT 
GGACCGTACG GATCCCTGGG AGTGGATTAT GAATTTGAAA AACTCCTTTG TAAAATATTT 
GGAGAGGATT TTATCGAACA GTTCAAGATC AAACGCCCGG CAGCCTGGGT TGACCTAATG 
ATTGCCTTTG AGTCTCGCAA AAGAGCTGCT GCACCAGATC GAACCAATCC CCTGAACATC 
ACTCTGCCCT TCTCCTTCAT TGACTACTAC AAGAAGTTCC GGGGCCACAG CGTGGAGCAT 
GCATTGAGGA AGAGCAATGT GGACTTTGTG AAGTGGTCCT CCCAGGGCAT GCTGAGGATG 
AGCCCAGATG CCATGAACGC CCTCTTCAAG CCGACCATCG ACAGCATCAT CGAGCACCTC 
CGGGACCTGT TTCAGAAGCC AGAGGTATCC ACGGTCAAGT TCCTCTTTCT GGTGGGTGGC 
TTTGCAGAGG CGCCCCTTTT GCAGCAGGCA GTGCAGACCG CTTTTGGCGA CAAATGCAGG 
ATCATCATCC CGCAGGATGT GGGCCTCACC ATCCTCAAGG GCGCTGTCCT CTTCGGCCTG 
GACCCTGCCG TTATCAAGGT GCGCCGGTCC CCTCTCACCT ACGGAGTAGG TGTGCTGAAC 
CGCTACGTGG AAGGGAAGCA CCCTCCCGAG AAGCTATTGG TCAAGGACGG CACACGCTGG 
TGCACCGACG TCTTCGACAA GTTCATCTCC GCCGACCAGT CTGTGGCTCT GGGGGAGCTG 
GTCAAGCGTA GCTACACCCC GGCCAAGCCT TCCCAGCTGG TCATCATCAT CAACATCTAC 
AGCTCTGAGC ATGATAACGT CAGCTTCATC ACTGACCCAG GGGTGAAAAA GTGTGGCACG 
CTCCGCTTGG ATCTCACGGG GAGCGGCGGC ACAGCGGTTC CCGCCCGCAG AGAGATCCAG 
ACCATCATGC AGTTCGGGGA CACTGAGATC AAGGCCACGG CCGTCGACAT AACCACCTCA 
AAGAGTGTCA AAGTGGGGAT CGACTTCTTA AATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527442.1
CDS	440..2218
Translation	MRRWEGGDPGVSNQKTPTTILLTPERKFHSFGYAARDFYHDLDPSEAKQWLYLEKFKMKLHTTGDLTMDTDLTAANGKKVKALEIFAYALQYFKEQALKELSDQAGSDFENSDVRWVITVPAIWKQPAKQFMREAAYQAGLASPENSEQLIIALEPEAASIYCRKLRLHQMIELSSKAVVNGYSASDTVGAGFAQAKEHVRRNRQSRTFLVENVIGEIWSELEEGDKYVVVDSGGGTVDLTVHQIRLPEGHLKELYKATGGPYGSLGVDYEFEKLLCKIFGEDFIEQFKIKRPAAWVDLMIAFESRKRAAAPDRTNPLNITLPFSFIDYYKKFRGHSVEHALRKSNVDFVKWSSQGMLRMSPDAMNALFKPTIDSIIEHLRDLFQKPEVSTVKFLFLVGGFAEAPLLQQAVQTAFGDKCRIIIPQDVGLTILKGAVLFGLDPAVIKVRRSPLTYGVGVLNRYVEGKHPPEKLLVKDGTRWCTDVFDKFISADQSVALGELVKRSYTPAKPSQLVIIINIYSSEHDNVSFITDPGVKKCGTLRLDLTGSGGTAVPARREIQTIMQFGDTEIKATAVDITTSKSVKVGIDFLNY

Target ORF information:

RefSeq Version	XM_006527379.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527379.4

ORF Insert Sequence:

ATGCGGAGAT GGGAAGGAGG TGACCCTGGT GTTTCCAACC AGAAGACCCC GACCACCATC 
TTGCTGACAC CAGAGAGGAA GTTCCACAGC TTTGGGTACG CTGCCAGGGA CTTCTACCAT 
GACCTGGACC CCAGTGAGGC CAAGCAATGG CTGTACTTGG AGAAGTTCAA GATGAAACTC 
CACACCACAG GGGACCTCAC CATGGACACA GACTTAACAG CAGCAAATGG CAAGAAGGTC 
AAAGCCCTCG AGATCTTCGC TTACGCCCTG CAGTACTTCA AGGAGCAGGC TCTGAAGGAA 
CTGAGTGACC AGGCAGGGTC GGACTTTGAG AACTCTGACG TCAGATGGGT CATCACAGTG 
CCTGCCATCT GGAAGCAACC AGCCAAGCAG TTCATGAGAG AAGCTGCCTA CCAGGCAGGC 
CTGGCCTCCC CCGAGAACTC GGAGCAGCTC ATCATTGCCC TGGAGCCCGA GGCCGCCTCC 
ATCTACTGCC GGAAGCTTCG GCTGCACCAG ATGATTGAGC TCAGCAGCAA GGCGGTGGTC 
AATGGGTACA GTGCCAGTGA CACAGTAGGA GCGGGGTTTG CACAGGCTAA GGAACATGTT 
CGACGTAACC GGCAGAGTCG GACCTTTTTG GTGGAGAATG TCATAGGAGA AATCTGGTCT 
GAGCTGGAGG AAGGGGACAA GTACGTGGTT GTTGACAGTG GCGGAGGCAC AGTAGACCTG 
ACCGTCCATC AGATACGGTT ACCAGAGGGG CACCTCAAGG AGCTGTACAA AGCAACAGGT 
GGACCGTACG GATCCCTGGG AGTGGATTAT GAATTTGAAA AACTCCTTTG TAAAATATTT 
GGAGAGGATT TTATCGAACA GTTCAAGATC AAACGCCCGG CAGCCTGGGT TGACCTAATG 
ATTGCCTTTG AGTCTCGCAA AAGAGCTGCT GCACCAGATC GAACCAATCC CCTGAACATC 
ACTCTGCCCT TCTCCTTCAT TGACTACTAC AAGAAGTTCC GGGGCCACAG CGTGGAGCAT 
GCATTGAGGA AGAGCAATGT GGACTTTGTG AAGTGGTCCT CCCAGGGCAT GCTGAGGATG 
AGCCCAGATG CCATGAACGC CCTCTTCAAG CCGACCATCG ACAGCATCAT CGAGCACCTC 
CGGGACCTGT TTCAGAAGCC AGAGGTATCC ACGGTCAAGT TCCTCTTTCT GGTGGGTGGC 
TTTGCAGAGG CGCCCCTTTT GCAGCAGGCA GTGCAGACCG CTTTTGGCGA CAAATGCAGG 
ATCATCATCC CGCAGGATGT GGGCCTCACC ATCCTCAAGG GCGCTGTCCT CTTCGGCCTG 
GACCCTGCCG TTATCAAGGT GCGCCGGTCC CCTCTCACCT ACGGAGTAGG TGTGCTGAAC 
CGCTACGTGG AAGGGAAGCA CCCTCCCGAG AAGCTATTGG TCAAGGACGG CACACGCTGG 
TGCACCGACG TCTTCGACAA GTTCATCTCC GCCGACCAGT CTGTGGCTCT GGGGGAGCTG 
GTCAAGCGTA GCTACACCCC GGCCAAGCCT TCCCAGCTGG TCATCATCAT CAACATCTAC 
AGCTCTGAGC ATGATAACGT CAGCTTCATC ACTGACCCAG GGGTGAAAAA GTGTGGCACG 
CTCCGCTTGG ATCTCACGGG GAGCGGCGGC ACAGCGGTTC CCGCCCGCAG AGAGATCCAG 
ACCATCATGC AGTTCGGGGA CACTGAGATC AAGGCCACGG CCGTCGACAT AACCACCTCA 
AAGAGTGTCA AAGTGGGGAT CGACTTCTTA AATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa12a ( XM_011247368.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245670.1 Mus musculus heat shock protein 12A (Hspa12a), transcript variant X2, mRNA.

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CloneID	OMu71010
Clone ID Related Accession (Same CDS sequence)	XM_011247368.2 , XM_011247368.3
Accession Version	XM_011247368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	heat shock 70 kDa protein 12A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247368.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGAG AAACAGCCCC CACATCTACA TATTCCTCTC CGGCCAGGAG TCTCGGGGAC 
ACAGGAATAA CACCTCTGTC CCCTTCCCAT ATCTTGAACG ATGCTGACCC CGTCTCTGAG 
CAGCAGACCT TTCTCGTGGT GGTGGCCATC GACTTTGGGA CCACGTCCAG TGGCTATGCC 
TACAGCTTTA CCAAGGAGCC AGAATGTATC CACGTGATGC GGAGATGGGA AGGAGGTGAC 
CCTGGTGTTT CCAACCAGAA GACCCCGACC ACCATCTTGC TGACACCAGA GAGGAAGTTC 
CACAGCTTTG GGTACGCTGC CAGGGACTTC TACCATGACC TGGACCCCAG TGAGGCCAAG 
CAATGGCTGT ACTTGGAGAA GTTCAAGATG AAACTCCACA CCACAGGGGA CCTCACCATG 
GACACAGACT TAACAGCAGC AAATGGCAAG AAGGTCAAAG CCCTCGAGAT CTTCGCTTAC 
GCCCTGCAGT ACTTCAAGGA GCAGGCTCTG AAGGAACTGA GTGACCAGGC AGGGTCGGAC 
TTTGAGAACT CTGACGTCAG ATGGGTCATC ACAGTGCCTG CCATCTGGAA GCAACCAGCC 
AAGCAGTTCA TGAGAGAAGC TGCCTACCAG GCAGGCCTGG CCTCCCCCGA GAACTCGGAG 
CAGCTCATCA TTGCCCTGGA GCCCGAGGCC GCCTCCATCT ACTGCCGGAA GCTTCGGCTG 
CACCAGATGA TTGAGCTCAG CAGCAAGGCG GTGGTCAATG GGTACAGTGC CAGTGACACA 
GTAGGAGCGG GGTTTGCACA GGCTAAGGAA CATGTTCGAC GTAACCGGCA GAGTCGGACC 
TTTTTGGTGG AGAATGTCAT AGGAGAAATC TGGTCTGAGC TGGAGGAAGG GGACAAGTAC 
GTGGTTGTTG ACAGTGGCGG AGGCACAGTA GACCTGACCG TCCATCAGAT ACGGTTACCA 
GAGGGGCACC TCAAGGAGCT GTACAAAGCA ACAGGTGGAC CGTACGGATC CCTGGGAGTG 
GATTATGAAT TTGAAAAACT CCTTTGTAAA ATATTTGGAG AGGATTTTAT CGAACAGTTC 
AAGATCAAAC GCCCGGCAGC CTGGGTTGAC CTAATGATTG CCTTTGAGTC TCGCAAAAGA 
GCTGCTGCAC CAGATCGAAC CAATCCCCTG AACATCACTC TGCCCTTCTC CTTCATTGAC 
TACTACAAGA AGTTCCGGGG CCACAGCGTG GAGCATGCAT TGAGGAAGAG CAATGTGGAC 
TTTGTGAAGT GGTCCTCCCA GGGCATGCTG AGGATGAGCC CAGATGCCAT GAACGCCCTC 
TTCAAGCCGA CCATCGACAG CATCATCGAG CACCTCCGGG ACCTGTTTCA GAAGCCAGAG 
GTATCCACGG TCAAGTTCCT CTTTCTGGTG GGTGGCTTTG CAGAGGCGCC CCTTTTGCAG 
CAGGCAGTGC AGACCGCTTT TGGCGACAAA TGCAGGATCA TCATCCCGCA GGATGTGGGC 
CTCACCATCC TCAAGGGCGC TGTCCTCTTC GGCCTGGACC CTGCCGTTAT CAAGGTGCGC 
CGGTCCCCTC TCACCTACGG AGTAGGTGTG CTGAACCGCT ACGTGGAAGG GAAGCACCCT 
CCCGAGAAGC TATTGGTCAA GGACGGCACA CGCTGGTGCA CCGACGTCTT CGACAAGTTC 
ATCTCCGCCG ACCAGTCTGT GGCTCTGGGG GAGCTGGTCA AGCGTAGCTA CACCCCGGCC 
AAGCCTTCCC AGCTGGTCAT CATCATCAAC ATCTACAGCT CTGAGCATGA TAACGTCAGC 
TTCATCACTG ACCCAGGGGT GAAAAAGTGT GGCACGCTCC GCTTGGATCT CACGGGGAGC 
GGCGGCACAG CGGTTCCCGC CCGCAGAGAG ATCCAGACCA TCATGCAGTT CGGGGACACT 
GAGATCAAGG CCACGGCCGT CGACATAACC ACCTCAAAGA GTGTCAAAGT GGGGATCGAC 
TTCTTAAATT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245670.1
CDS	245..2239
Translation	MTRETAPTSTYSSPARSLGDTGITPLSPSHILNDADPVSEQQTFLVVVAIDFGTTSSGYAYSFTKEPECIHVMRRWEGGDPGVSNQKTPTTILLTPERKFHSFGYAARDFYHDLDPSEAKQWLYLEKFKMKLHTTGDLTMDTDLTAANGKKVKALEIFAYALQYFKEQALKELSDQAGSDFENSDVRWVITVPAIWKQPAKQFMREAAYQAGLASPENSEQLIIALEPEAASIYCRKLRLHQMIELSSKAVVNGYSASDTVGAGFAQAKEHVRRNRQSRTFLVENVIGEIWSELEEGDKYVVVDSGGGTVDLTVHQIRLPEGHLKELYKATGGPYGSLGVDYEFEKLLCKIFGEDFIEQFKIKRPAAWVDLMIAFESRKRAAAPDRTNPLNITLPFSFIDYYKKFRGHSVEHALRKSNVDFVKWSSQGMLRMSPDAMNALFKPTIDSIIEHLRDLFQKPEVSTVKFLFLVGGFAEAPLLQQAVQTAFGDKCRIIIPQDVGLTILKGAVLFGLDPAVIKVRRSPLTYGVGVLNRYVEGKHPPEKLLVKDGTRWCTDVFDKFISADQSVALGELVKRSYTPAKPSQLVIIINIYSSEHDNVSFITDPGVKKCGTLRLDLTGSGGTAVPARREIQTIMQFGDTEIKATAVDITTSKSVKVGIDFLNY

Target ORF information:

RefSeq Version	XM_011247368.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 12A (Hspa12a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247368.3

ORF Insert Sequence:

ATGACCAGAG AAACAGCCCC CACATCTACA TATTCCTCTC CGGCCAGGAG TCTCGGGGAC 
ACAGGAATAA CACCTCTGTC CCCTTCCCAT ATCTTGAACG ATGCTGACCC CGTCTCTGAG 
CAGCAGACCT TTCTCGTGGT GGTGGCCATC GACTTTGGGA CCACGTCCAG TGGCTATGCC 
TACAGCTTTA CCAAGGAGCC AGAATGTATC CACGTGATGC GGAGATGGGA AGGAGGTGAC 
CCTGGTGTTT CCAACCAGAA GACCCCGACC ACCATCTTGC TGACACCAGA GAGGAAGTTC 
CACAGCTTTG GGTACGCTGC CAGGGACTTC TACCATGACC TGGACCCCAG TGAGGCCAAG 
CAATGGCTGT ACTTGGAGAA GTTCAAGATG AAACTCCACA CCACAGGGGA CCTCACCATG 
GACACAGACT TAACAGCAGC AAATGGCAAG AAGGTCAAAG CCCTCGAGAT CTTCGCTTAC 
GCCCTGCAGT ACTTCAAGGA GCAGGCTCTG AAGGAACTGA GTGACCAGGC AGGGTCGGAC 
TTTGAGAACT CTGACGTCAG ATGGGTCATC ACAGTGCCTG CCATCTGGAA GCAACCAGCC 
AAGCAGTTCA TGAGAGAAGC TGCCTACCAG GCAGGCCTGG CCTCCCCCGA GAACTCGGAG 
CAGCTCATCA TTGCCCTGGA GCCCGAGGCC GCCTCCATCT ACTGCCGGAA GCTTCGGCTG 
CACCAGATGA TTGAGCTCAG CAGCAAGGCG GTGGTCAATG GGTACAGTGC CAGTGACACA 
GTAGGAGCGG GGTTTGCACA GGCTAAGGAA CATGTTCGAC GTAACCGGCA GAGTCGGACC 
TTTTTGGTGG AGAATGTCAT AGGAGAAATC TGGTCTGAGC TGGAGGAAGG GGACAAGTAC 
GTGGTTGTTG ACAGTGGCGG AGGCACAGTA GACCTGACCG TCCATCAGAT ACGGTTACCA 
GAGGGGCACC TCAAGGAGCT GTACAAAGCA ACAGGTGGAC CGTACGGATC CCTGGGAGTG 
GATTATGAAT TTGAAAAACT CCTTTGTAAA ATATTTGGAG AGGATTTTAT CGAACAGTTC 
AAGATCAAAC GCCCGGCAGC CTGGGTTGAC CTAATGATTG CCTTTGAGTC TCGCAAAAGA 
GCTGCTGCAC CAGATCGAAC CAATCCCCTG AACATCACTC TGCCCTTCTC CTTCATTGAC 
TACTACAAGA AGTTCCGGGG CCACAGCGTG GAGCATGCAT TGAGGAAGAG CAATGTGGAC 
TTTGTGAAGT GGTCCTCCCA GGGCATGCTG AGGATGAGCC CAGATGCCAT GAACGCCCTC 
TTCAAGCCGA CCATCGACAG CATCATCGAG CACCTCCGGG ACCTGTTTCA GAAGCCAGAG 
GTATCCACGG TCAAGTTCCT CTTTCTGGTG GGTGGCTTTG CAGAGGCGCC CCTTTTGCAG 
CAGGCAGTGC AGACCGCTTT TGGCGACAAA TGCAGGATCA TCATCCCGCA GGATGTGGGC 
CTCACCATCC TCAAGGGCGC TGTCCTCTTC GGCCTGGACC CTGCCGTTAT CAAGGTGCGC 
CGGTCCCCTC TCACCTACGG AGTAGGTGTG CTGAACCGCT ACGTGGAAGG GAAGCACCCT 
CCCGAGAAGC TATTGGTCAA GGACGGCACA CGCTGGTGCA CCGACGTCTT CGACAAGTTC 
ATCTCCGCCG ACCAGTCTGT GGCTCTGGGG GAGCTGGTCA AGCGTAGCTA CACCCCGGCC 
AAGCCTTCCC AGCTGGTCAT CATCATCAAC ATCTACAGCT CTGAGCATGA TAACGTCAGC 
TTCATCACTG ACCCAGGGGT GAAAAAGTGT GGCACGCTCC GCTTGGATCT CACGGGGAGC 
GGCGGCACAG CGGTTCCCGC CCGCAGAGAG ATCCAGACCA TCATGCAGTT CGGGGACACT 
GAGATCAAGG CCACGGCCGT CGACATAACC ACCTCAAAGA GTGTCAAAGT GGGGATCGAC 
TTCTTAAATT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)
Munton RP,Tweedie-Cullen R,Livingstone-Zatchej M,Weinandy F,Waidelich M,Longo D,Gehrig P,Potthast F,Rutishauser D,Gerrits B,Panse C,Schlapbach R,Mansuy IM

Identification of cathepsin K as a novel marker of adiposity in white adipose tissue.
Journal of cellular physiology195(2)309-21(2003 May)
Chiellini C,Costa M,Novelli SE,Amri EZ,Benzi L,Bertacca A,Cohen P,Del Prato S,Friedman JM,Maffei M

Two Hsp70 family members expressed in atherosclerotic lesions.
Proceedings of the National Academy of Sciences of the United States of America100(3)1256-61(2003 Feb)
Han Z,Truong QA,Park S,Breslow JL

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Hspa12a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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