Ndc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndc1
Entrez Gene ID	72787
Full Name	NDC1 transmembrane nucleoporin
Synonyms	2810475A17Rik,AI450313,Tmem48,sks
General protein information	Preferred Names NDC1 transmembrane nucleoporin Names nucleoporin NDC1 transmembrane protein 48
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_028355.3	NP_082631.1	nucleoporin NDC1
XM_006503423.2	XP_006503486.1	nucleoporin NDC1 isoform X1
NM_028355.4	NP_082631.1	nucleoporin NDC1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TMEM48	XP_001235442.3
Pan troglodytes (chimpanzee)	TMEM48	XP_513417.4
Drosophila melanogaster (fruit fly)	CG5857	NP_651191.1
Homo sapiens (human)	NDC1	NP_060557.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004558	XP_313856.5
Xenopus tropicalis (tropical clawed frog)	ndc1	NP_001016813.1
Canis lupus familiaris (dog)	NDC1	XP_003434706.1
Bos taurus (cattle)	NDC1	XP_003585949.2
Mus musculus (house mouse)	Ndc1	NP_082631.1
Rattus norvegicus (Norway rat)	Ndc1	NP_001020194.1
Danio rerio (zebrafish)	ndc1	NP_956237.1

Gene Ontology
Bibliography

Related articles in PubMed

SEPT12-NDC1 Complexes Are Required for Mammalian Spermiogenesis.
Lai TH, Wu YY, Wang YY, Chen MF, Wang P, Chen TM, Wu YN, Chiang HS, Kuo PL, Lin YH
International journal of molecular sciences17(11)(2016 Nov)

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Lewandowski JP, Du F, Zhang S, Powell MB, Falkenstein KN, Ji H, Vokes SA
Developmental biology406(1)92-103(2015 Oct)

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
Fairfield H, Srivastava A, Ananda G, Liu R, Kircher M, Lakshminarayana A, Harris BS, Karst SY, Dionne LA, Kane CC, Curtain M, Berry ML, Ward-Bailey PF, Greenstein I, Byers C, Czechanski A, Sharp J, Palmer K, Gudis P, Martin W, Tadenev A, Bogdanik L, Pratt CH, Chang B, Schroeder DG, Cox GA, Cliften P, Milbrandt J, Murray S, Burgess R, Bergstrom DE, Donahue LR, Hamamy H, Masri A, Santoni FA, Makrythanasis P, Antonarakis SE, Shendure J, Reinholdt LG
Genome research25(7)948-57(2015 Jul)

A mutation in the nuclear pore complex gene Tmem48 causes gametogenesis defects in skeletal fusions with sterility (sks) mice.
Akiyama K, Noguchi J, Hirose M, Kajita S, Katayama K, Khalaj M, Tsuji T, Fairfield H, Byers C, Reinholdt L, Ogura A, Kunieda T
The Journal of biological chemistry288(44)31830-41(2013 Nov)

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Hutchins JR, Toyoda Y, Hegemann B, Poser I, H?rich? JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM
Science (New York, N.Y.)328(5978)593-9(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In mice sperm with mutated SEPT12, NDC1 dispersed around the manchette region of the sperm head and annulus, compared with concentrating at the sperm neck of wild-type sperm.
Title: SEPT12-NDC1 Complexes Are Required for Mammalian Spermiogenesis.

A mutation in the nuclear pore complex gene Tmem48 causes gametogenesis defects in skeletal fusions with sterility (sks) mice.
Title: A mutation in the nuclear pore complex gene Tmem48 causes gametogenesis defects in skeletal fusions with sterility (sks) mice.

The following Ndc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu33837	XM_006503423.2 Latest version!	Mus musculus NDC1 transmembrane nucleoporin (Ndc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu18773	NM_028355.4 Latest version!	Mus musculus NDC1 transmembrane nucleoporin (Ndc1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu18773	NM_028355.3	Mus musculus NDC1 transmembrane nucleoporin (Ndc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$398.30 $569.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndc1 ( XM_006503423.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503486.1 Mus musculus NDC1 transmembrane nucleoporin (Ndc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33837
Clone ID Related Accession (Same CDS sequence)	XM_006503423.2
Accession Version	XM_006503423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nucleoporin NDC1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAATGG CTGTGGCTTG GTGTGCGGCC ATCATGACCA AGGGCCAGTA CAGCTCTCTC 
GTGGTTCCCT GCACTGGAAC TGAGAGCTTA GATAGCCCTG CTGCTCAGAC CTGCTTGAAT 
GAATATCATC TGTTTTTCCT ACTGTCCGGA GCATTCATGG GCTATAGCTA TAGTCTTCTG 
TATTTCATTA ACAACATGAA TTATCTCCCG TTTCCTATTA TACAGCAGTA CAAATTTTTG 
CGTTTCCGGA GATCTCTCCT CTTACTAGTT AAACATAGCT GTGTGGAATC CCTGTTCATG 
GTCAGAAATT TCTGCATTGT GTATTATTTT TTTGGCCATA TTCCCAAAGC ATGGATTAGC 
ACTGCTCTGG ACCTTCACAC AGATGAGCAG GCTCACAGGC CTCTTGACAC GATAGGCGGT 
CTCCTGAACG TCTCATTACT CTACCACGTC TGGCTGTGTG GCGTCTTCCT CTTGGTGACT 
TGGTATAGCT CATGGATACT CTTCAAAATC TATGCCACCG AGGCGCATGT GTTCCCTGTT 
CAGCCGCCTT TTGCAGAAGC CTCAGATGAA TGCCTTCCTA AAGTCTTAAA CAGCAACCCC 
CCACGCATCG TAAAGTATTT GGCCTTACAG GACCTGATGC TGCTTTCTCA ATATTCTCCT 
TCTCGGAGGC AAGAAGTTTT CAGTCTCAGC CAACCAGGTG GACATCCCCA CAATTGGACT 
GCTATTTCGA GGGAGTGTTT GAATCTTTTA AATGATATGA CTCAGAAACT GGTTCTCTAC 
CAAGAAGCTG CTGCTACAAA TGGGAGGATG TATTCATCAT ACTCAGTGGA ACCCAAGAAA 
CTGAGCTCTG CAGAAGAAAC TGCTTTTCAG ACTCCAAAAC CTAGTCAGAC GCCTTCCGTG 
CCGCCGTTAG TTAAAACTTC ACTGTTTTCT CCAAAATTAT CTACACCCAA TGTTTCAAGT 
CCTTTTGGGA CCCCGTTTGG CTCCAGCGTA GTGAACCGAA TGGCTGGAAT TCTTGATGTA 
AACCCCTTCT CTGGGTCTCC CCAAAGTCCT CAGCTCATAA GAAGAGGACC GAGGCTGTGG 
ACTCACACTT CTGATCAACA AGTGTCTGCA ATTTCTAACC CATCTCCTTG CGCCTCTGTT 
ACTGCTGAGG GTAAGACAGT GAGACAGCCC AGTGTGATCT ACTCGTGGAT TCAGAATAAA 
CGAGAACAGA TTAAGAATTT CTTGTCAAAA CGGGTGCTGA TAATGTACTT TTTCAGTAAG 
CACCCAGAGG CCTCCATTCA GGCTGTTTTT TCAGATGCCC AAATGCATAT TTGGGCTTTA 
GAAGGTCTGT CTCACTTAGT AGCAGCGTCA TTTACAGAAG ATAGATTTGG AGTTGTCCAG 
ACCACACTCC CAGCTATCCT TCATACGCTG TTGACTCTGC AGGAGGCTGT CGACAAGTAC 
TTCAAGCTGC CTCATGCTTC TAGTAAACCA CCCCGAGCTT CAGGAAGCCT TGTGGACACG 
TCCTACAAAA CATTGAGATT TGCTTTCAGG GCCTCCCTGA AAACCGCTAT CTACCGTATA 
ACCACCACGT TTGGTGAACA TCTGAACGCT GTGCAGGCAT CTGCAGAACA TCAGAAAAGA 
CTTCAGCAAT TCTTGGAGTT CAAAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503486.1
CDS	572..2221
Translation	MGMAVAWCAAIMTKGQYSSLVVPCTGTESLDSPAAQTCLNEYHLFFLLSGAFMGYSYSLLYFINNMNYLPFPIIQQYKFLRFRRSLLLLVKHSCVESLFMVRNFCIVYYFFGHIPKAWISTALDLHTDEQAHRPLDTIGGLLNVSLLYHVWLCGVFLLVTWYSSWILFKIYATEAHVFPVQPPFAEASDECLPKVLNSNPPRIVKYLALQDLMLLSQYSPSRRQEVFSLSQPGGHPHNWTAISRECLNLLNDMTQKLVLYQEAAATNGRMYSSYSVEPKKLSSAEETAFQTPKPSQTPSVPPLVKTSLFSPKLSTPNVSSPFGTPFGSSVVNRMAGILDVNPFSGSPQSPQLIRRGPRLWTHTSDQQVSAISNPSPCASVTAEGKTVRQPSVIYSWIQNKREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQMHIWALEGLSHLVAASFTEDRFGVVQTTLPAILHTLLTLQEAVDKYFKLPHASSKPPRASGSLVDTSYKTLRFAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE

Target ORF information:

RefSeq Version	XM_006503423.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NDC1 transmembrane nucleoporin (Ndc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503423.2

ORF Insert Sequence:

ATGGGAATGG CTGTGGCTTG GTGTGCGGCC ATCATGACCA AGGGCCAGTA CAGCTCTCTC 
GTGGTTCCCT GCACTGGAAC TGAGAGCTTA GATAGCCCTG CTGCTCAGAC CTGCTTGAAT 
GAATATCATC TGTTTTTCCT ACTGTCCGGA GCATTCATGG GCTATAGCTA TAGTCTTCTG 
TATTTCATTA ACAACATGAA TTATCTCCCG TTTCCTATTA TACAGCAGTA CAAATTTTTG 
CGTTTCCGGA GATCTCTCCT CTTACTAGTT AAACATAGCT GTGTGGAATC CCTGTTCATG 
GTCAGAAATT TCTGCATTGT GTATTATTTT TTTGGCCATA TTCCCAAAGC ATGGATTAGC 
ACTGCTCTGG ACCTTCACAC AGATGAGCAG GCTCACAGGC CTCTTGACAC GATAGGCGGT 
CTCCTGAACG TCTCATTACT CTACCACGTC TGGCTGTGTG GCGTCTTCCT CTTGGTGACT 
TGGTATAGCT CATGGATACT CTTCAAAATC TATGCCACCG AGGCGCATGT GTTCCCTGTT 
CAGCCGCCTT TTGCAGAAGC CTCAGATGAA TGCCTTCCTA AAGTCTTAAA CAGCAACCCC 
CCACGCATCG TAAAGTATTT GGCCTTACAG GACCTGATGC TGCTTTCTCA ATATTCTCCT 
TCTCGGAGGC AAGAAGTTTT CAGTCTCAGC CAACCAGGTG GACATCCCCA CAATTGGACT 
GCTATTTCGA GGGAGTGTTT GAATCTTTTA AATGATATGA CTCAGAAACT GGTTCTCTAC 
CAAGAAGCTG CTGCTACAAA TGGGAGGATG TATTCATCAT ACTCAGTGGA ACCCAAGAAA 
CTGAGCTCTG CAGAAGAAAC TGCTTTTCAG ACTCCAAAAC CTAGTCAGAC GCCTTCCGTG 
CCGCCGTTAG TTAAAACTTC ACTGTTTTCT CCAAAATTAT CTACACCCAA TGTTTCAAGT 
CCTTTTGGGA CCCCGTTTGG CTCCAGCGTA GTGAACCGAA TGGCTGGAAT TCTTGATGTA 
AACCCCTTCT CTGGGTCTCC CCAAAGTCCT CAGCTCATAA GAAGAGGACC GAGGCTGTGG 
ACTCACACTT CTGATCAACA AGTGTCTGCA ATTTCTAACC CATCTCCTTG CGCCTCTGTT 
ACTGCTGAGG GTAAGACAGT GAGACAGCCC AGTGTGATCT ACTCGTGGAT TCAGAATAAA 
CGAGAACAGA TTAAGAATTT CTTGTCAAAA CGGGTGCTGA TAATGTACTT TTTCAGTAAG 
CACCCAGAGG CCTCCATTCA GGCTGTTTTT TCAGATGCCC AAATGCATAT TTGGGCTTTA 
GAAGGTCTGT CTCACTTAGT AGCAGCGTCA TTTACAGAAG ATAGATTTGG AGTTGTCCAG 
ACCACACTCC CAGCTATCCT TCATACGCTG TTGACTCTGC AGGAGGCTGT CGACAAGTAC 
TTCAAGCTGC CTCATGCTTC TAGTAAACCA CCCCGAGCTT CAGGAAGCCT TGTGGACACG 
TCCTACAAAA CATTGAGATT TGCTTTCAGG GCCTCCCTGA AAACCGCTAT CTACCGTATA 
ACCACCACGT TTGGTGAACA TCTGAACGCT GTGCAGGCAT CTGCAGAACA TCAGAAAAGA 
CTTCAGCAAT TCTTGGAGTT CAAAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndc1 ( NM_028355.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082631.1 Mus musculus NDC1 transmembrane nucleoporin (Ndc1), mRNA.

Price & Availability↑

CloneID	OMu18773
Clone ID Related Accession (Same CDS sequence)	NM_028355.3 , NM_028355.4
Accession Version	NM_028355.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	nucleoporin NDC1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK144734.1. On Apr 6, 2007 this sequence version replaced NM_028355.2. ##Evidence-Data-START## Transcript exon combination :: AK144734.1, SRR1660823.153071.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACGG CTGCGAGCGG GCCCTGCGCC GGCGGGTCGC CGCGGGACAT CCTTTGGCGC 
GTTTTGGGTT GGAGGATAGT TACCAGCATC GTTTGGTCAG TAGTGCTTCT ACCCGTCTGC 
ATCACAGCCT TTATAGTCTT AAGCAGCATC AATTTATTCC ACCCTATACA GTGGCTGTCA 
GATTCTTGCA ATGACTTCTA TAGTTCCCAA GTCATCTTTC ACCTCCTGCT GCTGGCCGTG 
GTCATTATAA TAATAAGTAT TTTCAATGTG GAATTCTATA CAGTTGTGCC CTCCATATCC 
GGCTCCAGAC TGGCCCTGAT AGCGCGGATC CTGCATCCCC AGCAGCTCAC GCACTCCTTC 
ATCCACGCCG CGATGGGAAT GGCTGTGGCT TGGTGTGCGG CCATCATGAC CAAGGGCCAG 
TACAGCTCTC TCGTGGTTCC CTGCACTGGA ACTGAGAGCT TAGATAGCCC TGCTGCTCAG 
ACCTGCTTGA ATGAATATCA TCTGTTTTTC CTACTGTCCG GAGCATTCAT GGGCTATAGC 
TATAGTCTTC TGTATTTCAT TAACAACATG AATTATCTCC CGTTTCCTAT TATACAGCAG 
TACAAATTTT TGCGTTTCCG GAGATCTCTC CTCTTACTAG TTAAACATAG CTGTGTGGAA 
TCCCTGTTCA TGGTCAGAAA TTTCTGCATT GTGTATTATT TTTTTGGCCA TATTCCCAAA 
GCATGGATTA GCACTGCTCT GGACCTTCAC ACAGATGAGC AGGCTCACAG GCCTCTTGAC 
ACGATAGGCG GTCTCCTGAA CGTCTCATTA CTCTACCACG TCTGGCTGTG TGGCGTCTTC 
CTCTTGGTGA CTTGGTATAG CTCATGGATA CTCTTCAAAA TCTATGCCAC CGAGGCGCAT 
GTGTTCCCTG TTCAGCCGCC TTTTGCAGAA GCCTCAGATG AATGCCTTCC TAAAGTCTTA 
AACAGCAACC CCCCACGCAT CGTAAAGTAT TTGGCCTTAC AGGACCTGAT GCTGCTTTCT 
CAATATTCTC CTTCTCGGAG GCAAGAAGTT TTCAGTCTCA GCCAACCAGG TGGACATCCC 
CACAATTGGA CTGCTATTTC GAGGGAGTGT TTGAATCTTT TAAATGATAT GACTCAGAAA 
CTGGTTCTCT ACCAAGAAGC TGCTGCTACA AATGGGAGGA TGTATTCATC ATACTCAGTG 
GAACCCAAGA AACTGAGCTC TGCAGAAGAA ACTGCTTTTC AGACTCCAAA ACCTAGTCAG 
ACGCCTTCCG TGCCGCCGTT AGTTAAAACT TCACTGTTTT CTCCAAAATT ATCTACACCC 
AATGTTTCAA GTCCTTTTGG GACCCCGTTT GGCTCCAGCG TAGTGAACCG AATGGCTGGA 
ATTCTTGATG TAAACCCCTT CTCTGGGTCT CCCCAAAGTC CTCAGCTCAT AAGAAGAGGA 
CCGAGGCTGT GGACTCACAC TTCTGATCAA CAAGTGTCTG CAATTTCTAA CCCATCTCCT 
TGCGCCTCTG TTACTGCTGA GGGTAAGACA GTGAGACAGC CCAGTGTGAT CTACTCGTGG 
ATTCAGAATA AACGAGAACA GATTAAGAAT TTCTTGTCAA AACGGGTGCT GATAATGTAC 
TTTTTCAGTA AGCACCCAGA GGCCTCCATT CAGGCTGTTT TTTCAGATGC CCAAATGCAT 
ATTTGGGCTT TAGAAGGTCT GTCTCACTTA GTAGCAGCGT CATTTACAGA AGATAGATTT 
GGAGTTGTCC AGACCACACT CCCAGCTATC CTTCATACGC TGTTGACTCT GCAGGAGGCT 
GTCGACAAGT ACTTCAAGCT GCCTCATGCT TCTAGTAAAC CACCCCGAGC TTCAGGAAGC 
CTTGTGGACA CGTCCTACAA AACATTGAGA TTTGCTTTCA GGGCCTCCCT GAAAACCGCT 
ATCTACCGTA TAACCACCAC GTTTGGTGAA CATCTGAACG CTGTGCAGGC ATCTGCAGAA 
CATCAGAAAA GACTTCAGCA ATTCTTGGAG TTCAAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082631.1
CDS	306..2327
Translation	MATAASGPCAGGSPRDILWRVLGWRIVTSIVWSVVLLPVCITAFIVLSSINLFHPIQWLSDSCNDFYSSQVIFHLLLLAVVIIIISIFNVEFYTVVPSISGSRLALIARILHPQQLTHSFIHAAMGMAVAWCAAIMTKGQYSSLVVPCTGTESLDSPAAQTCLNEYHLFFLLSGAFMGYSYSLLYFINNMNYLPFPIIQQYKFLRFRRSLLLLVKHSCVESLFMVRNFCIVYYFFGHIPKAWISTALDLHTDEQAHRPLDTIGGLLNVSLLYHVWLCGVFLLVTWYSSWILFKIYATEAHVFPVQPPFAEASDECLPKVLNSNPPRIVKYLALQDLMLLSQYSPSRRQEVFSLSQPGGHPHNWTAISRECLNLLNDMTQKLVLYQEAAATNGRMYSSYSVEPKKLSSAEETAFQTPKPSQTPSVPPLVKTSLFSPKLSTPNVSSPFGTPFGSSVVNRMAGILDVNPFSGSPQSPQLIRRGPRLWTHTSDQQVSAISNPSPCASVTAEGKTVRQPSVIYSWIQNKREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQMHIWALEGLSHLVAASFTEDRFGVVQTTLPAILHTLLTLQEAVDKYFKLPHASSKPPRASGSLVDTSYKTLRFAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE

Target ORF information:

RefSeq Version	NM_028355.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NDC1 transmembrane nucleoporin (Ndc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028355.3

ORF Insert Sequence:

ATGGCCACGG CTGCGAGCGG GCCCTGCGCC GGCGGGTCGC CGCGGGACAT CCTTTGGCGC 
GTTTTGGGTT GGAGGATAGT TACCAGCATC GTTTGGTCAG TAGTGCTTCT ACCCGTCTGC 
ATCACAGCCT TTATAGTCTT AAGCAGCATC AATTTATTCC ACCCTATACA GTGGCTGTCA 
GATTCTTGCA ATGACTTCTA TAGTTCCCAA GTCATCTTTC ACCTCCTGCT GCTGGCCGTG 
GTCATTATAA TAATAAGTAT TTTCAATGTG GAATTCTATA CAGTTGTGCC CTCCATATCC 
GGCTCCAGAC TGGCCCTGAT AGCGCGGATC CTGCATCCCC AGCAGCTCAC GCACTCCTTC 
ATCCACGCCG CGATGGGAAT GGCTGTGGCT TGGTGTGCGG CCATCATGAC CAAGGGCCAG 
TACAGCTCTC TCGTGGTTCC CTGCACTGGA ACTGAGAGCT TAGATAGCCC TGCTGCTCAG 
ACCTGCTTGA ATGAATATCA TCTGTTTTTC CTACTGTCCG GAGCATTCAT GGGCTATAGC 
TATAGTCTTC TGTATTTCAT TAACAACATG AATTATCTCC CGTTTCCTAT TATACAGCAG 
TACAAATTTT TGCGTTTCCG GAGATCTCTC CTCTTACTAG TTAAACATAG CTGTGTGGAA 
TCCCTGTTCA TGGTCAGAAA TTTCTGCATT GTGTATTATT TTTTTGGCCA TATTCCCAAA 
GCATGGATTA GCACTGCTCT GGACCTTCAC ACAGATGAGC AGGCTCACAG GCCTCTTGAC 
ACGATAGGCG GTCTCCTGAA CGTCTCATTA CTCTACCACG TCTGGCTGTG TGGCGTCTTC 
CTCTTGGTGA CTTGGTATAG CTCATGGATA CTCTTCAAAA TCTATGCCAC CGAGGCGCAT 
GTGTTCCCTG TTCAGCCGCC TTTTGCAGAA GCCTCAGATG AATGCCTTCC TAAAGTCTTA 
AACAGCAACC CCCCACGCAT CGTAAAGTAT TTGGCCTTAC AGGACCTGAT GCTGCTTTCT 
CAATATTCTC CTTCTCGGAG GCAAGAAGTT TTCAGTCTCA GCCAACCAGG TGGACATCCC 
CACAATTGGA CTGCTATTTC GAGGGAGTGT TTGAATCTTT TAAATGATAT GACTCAGAAA 
CTGGTTCTCT ACCAAGAAGC TGCTGCTACA AATGGGAGGA TGTATTCATC ATACTCAGTG 
GAACCCAAGA AACTGAGCTC TGCAGAAGAA ACTGCTTTTC AGACTCCAAA ACCTAGTCAG 
ACGCCTTCCG TGCCGCCGTT AGTTAAAACT TCACTGTTTT CTCCAAAATT ATCTACACCC 
AATGTTTCAA GTCCTTTTGG GACCCCGTTT GGCTCCAGCG TAGTGAACCG AATGGCTGGA 
ATTCTTGATG TAAACCCCTT CTCTGGGTCT CCCCAAAGTC CTCAGCTCAT AAGAAGAGGA 
CCGAGGCTGT GGACTCACAC TTCTGATCAA CAAGTGTCTG CAATTTCTAA CCCATCTCCT 
TGCGCCTCTG TTACTGCTGA GGGTAAGACA GTGAGACAGC CCAGTGTGAT CTACTCGTGG 
ATTCAGAATA AACGAGAACA GATTAAGAAT TTCTTGTCAA AACGGGTGCT GATAATGTAC 
TTTTTCAGTA AGCACCCAGA GGCCTCCATT CAGGCTGTTT TTTCAGATGC CCAAATGCAT 
ATTTGGGCTT TAGAAGGTCT GTCTCACTTA GTAGCAGCGT CATTTACAGA AGATAGATTT 
GGAGTTGTCC AGACCACACT CCCAGCTATC CTTCATACGC TGTTGACTCT GCAGGAGGCT 
GTCGACAAGT ACTTCAAGCT GCCTCATGCT TCTAGTAAAC CACCCCGAGC TTCAGGAAGC 
CTTGTGGACA CGTCCTACAA AACATTGAGA TTTGCTTTCA GGGCCTCCCT GAAAACCGCT 
ATCTACCGTA TAACCACCAC GTTTGGTGAA CATCTGAACG CTGTGCAGGC ATCTGCAGAA 
CATCAGAAAA GACTTCAGCA ATTCTTGGAG TTCAAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndc1 ( NM_028355.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082631.1 Mus musculus NDC1 transmembrane nucleoporin (Ndc1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18773
Clone ID Related Accession (Same CDS sequence)	NM_028355.3 , NM_028355.4
Accession Version	NM_028355.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-12
Organism	Mus musculus(house mouse)
Product	nucleoporin NDC1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL626784.11. On Dec 13, 2019 this sequence version replaced NM_028355.3. Transcript Variant: This variant (1) represents the protein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: AK144734.1, BC021337.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCCACGG CTGCGAGCGG GCCCTGCGCC GGCGGGTCGC CGCGGGACAT CCTTTGGCGC 
GTTTTGGGTT GGAGGATAGT TACCAGCATC GTTTGGTCAG TAGTGCTTCT ACCCGTCTGC 
ATCACAGCCT TTATAGTCTT AAGCAGCATC AATTTATTCC ACCCTATACA GTGGCTGTCA 
GATTCTTGCA ATGACTTCTA TAGTTCCCAA GTCATCTTTC ACCTCCTGCT GCTGGCCGTG 
GTCATTATAA TAATAAGTAT TTTCAATGTG GAATTCTATA CAGTTGTGCC CTCCATATCC 
GGCTCCAGAC TGGCCCTGAT AGCGCGGATC CTGCATCCCC AGCAGCTCAC GCACTCCTTC 
ATCCACGCCG CGATGGGAAT GGCTGTGGCT TGGTGTGCGG CCATCATGAC CAAGGGCCAG 
TACAGCTCTC TCGTGGTTCC CTGCACTGGA ACTGAGAGCT TAGATAGCCC TGCTGCTCAG 
ACCTGCTTGA ATGAATATCA TCTGTTTTTC CTACTGTCCG GAGCATTCAT GGGCTATAGC 
TATAGTCTTC TGTATTTCAT TAACAACATG AATTATCTCC CGTTTCCTAT TATACAGCAG 
TACAAATTTT TGCGTTTCCG GAGATCTCTC CTCTTACTAG TTAAACATAG CTGTGTGGAA 
TCCCTGTTCA TGGTCAGAAA TTTCTGCATT GTGTATTATT TTTTTGGCCA TATTCCCAAA 
GCATGGATTA GCACTGCTCT GGACCTTCAC ACAGATGAGC AGGCTCACAG GCCTCTTGAC 
ACGATAGGCG GTCTCCTGAA CGTCTCATTA CTCTACCACG TCTGGCTGTG TGGCGTCTTC 
CTCTTGGTGA CTTGGTATAG CTCATGGATA CTCTTCAAAA TCTATGCCAC CGAGGCGCAT 
GTGTTCCCTG TTCAGCCGCC TTTTGCAGAA GCCTCAGATG AATGCCTTCC TAAAGTCTTA 
AACAGCAACC CCCCACGCAT CGTAAAGTAT TTGGCCTTAC AGGACCTGAT GCTGCTTTCT 
CAATATTCTC CTTCTCGGAG GCAAGAAGTT TTCAGTCTCA GCCAACCAGG TGGACATCCC 
CACAATTGGA CTGCTATTTC GAGGGAGTGT TTGAATCTTT TAAATGATAT GACTCAGAAA 
CTGGTTCTCT ACCAAGAAGC TGCTGCTACA AATGGGAGGA TGTATTCATC ATACTCAGTG 
GAACCCAAGA AACTGAGCTC TGCAGAAGAA ACTGCTTTTC AGACTCCAAA ACCTAGTCAG 
ACGCCTTCCG TGCCGCCGTT AGTTAAAACT TCACTGTTTT CTCCAAAATT ATCTACACCC 
AATGTTTCAA GTCCTTTTGG GACCCCGTTT GGCTCCAGCG TAGTGAACCG AATGGCTGGA 
ATTCTTGATG TAAACCCCTT CTCTGGGTCT CCCCAAAGTC CTCAGCTCAT AAGAAGAGGA 
CCGAGGCTGT GGACTCACAC TTCTGATCAA CAAGTGTCTG CAATTTCTAA CCCATCTCCT 
TGCGCCTCTG TTACTGCTGA GGGTAAGACA GTGAGACAGC CCAGTGTGAT CTACTCGTGG 
ATTCAGAATA AACGAGAACA GATTAAGAAT TTCTTGTCAA AACGGGTGCT GATAATGTAC 
TTTTTCAGTA AGCACCCAGA GGCCTCCATT CAGGCTGTTT TTTCAGATGC CCAAATGCAT 
ATTTGGGCTT TAGAAGGTCT GTCTCACTTA GTAGCAGCGT CATTTACAGA AGATAGATTT 
GGAGTTGTCC AGACCACACT CCCAGCTATC CTTCATACGC TGTTGACTCT GCAGGAGGCT 
GTCGACAAGT ACTTCAAGCT GCCTCATGCT TCTAGTAAAC CACCCCGAGC TTCAGGAAGC 
CTTGTGGACA CGTCCTACAA AACATTGAGA TTTGCTTTCA GGGCCTCCCT GAAAACCGCT 
ATCTACCGTA TAACCACCAC GTTTGGTGAA CATCTGAACG CTGTGCAGGC ATCTGCAGAA 
CATCAGAAAA GACTTCAGCA ATTCTTGGAG TTCAAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082631.1
CDS	43..2064
Translation	MATAASGPCAGGSPRDILWRVLGWRIVTSIVWSVVLLPVCITAFIVLSSINLFHPIQWLSDSCNDFYSSQVIFHLLLLAVVIIIISIFNVEFYTVVPSISGSRLALIARILHPQQLTHSFIHAAMGMAVAWCAAIMTKGQYSSLVVPCTGTESLDSPAAQTCLNEYHLFFLLSGAFMGYSYSLLYFINNMNYLPFPIIQQYKFLRFRRSLLLLVKHSCVESLFMVRNFCIVYYFFGHIPKAWISTALDLHTDEQAHRPLDTIGGLLNVSLLYHVWLCGVFLLVTWYSSWILFKIYATEAHVFPVQPPFAEASDECLPKVLNSNPPRIVKYLALQDLMLLSQYSPSRRQEVFSLSQPGGHPHNWTAISRECLNLLNDMTQKLVLYQEAAATNGRMYSSYSVEPKKLSSAEETAFQTPKPSQTPSVPPLVKTSLFSPKLSTPNVSSPFGTPFGSSVVNRMAGILDVNPFSGSPQSPQLIRRGPRLWTHTSDQQVSAISNPSPCASVTAEGKTVRQPSVIYSWIQNKREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQMHIWALEGLSHLVAASFTEDRFGVVQTTLPAILHTLLTLQEAVDKYFKLPHASSKPPRASGSLVDTSYKTLRFAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE

Target ORF information:

RefSeq Version	NM_028355.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NDC1 transmembrane nucleoporin (Ndc1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028355.4

ORF Insert Sequence:

ATGGCCACGG CTGCGAGCGG GCCCTGCGCC GGCGGGTCGC CGCGGGACAT CCTTTGGCGC 
GTTTTGGGTT GGAGGATAGT TACCAGCATC GTTTGGTCAG TAGTGCTTCT ACCCGTCTGC 
ATCACAGCCT TTATAGTCTT AAGCAGCATC AATTTATTCC ACCCTATACA GTGGCTGTCA 
GATTCTTGCA ATGACTTCTA TAGTTCCCAA GTCATCTTTC ACCTCCTGCT GCTGGCCGTG 
GTCATTATAA TAATAAGTAT TTTCAATGTG GAATTCTATA CAGTTGTGCC CTCCATATCC 
GGCTCCAGAC TGGCCCTGAT AGCGCGGATC CTGCATCCCC AGCAGCTCAC GCACTCCTTC 
ATCCACGCCG CGATGGGAAT GGCTGTGGCT TGGTGTGCGG CCATCATGAC CAAGGGCCAG 
TACAGCTCTC TCGTGGTTCC CTGCACTGGA ACTGAGAGCT TAGATAGCCC TGCTGCTCAG 
ACCTGCTTGA ATGAATATCA TCTGTTTTTC CTACTGTCCG GAGCATTCAT GGGCTATAGC 
TATAGTCTTC TGTATTTCAT TAACAACATG AATTATCTCC CGTTTCCTAT TATACAGCAG 
TACAAATTTT TGCGTTTCCG GAGATCTCTC CTCTTACTAG TTAAACATAG CTGTGTGGAA 
TCCCTGTTCA TGGTCAGAAA TTTCTGCATT GTGTATTATT TTTTTGGCCA TATTCCCAAA 
GCATGGATTA GCACTGCTCT GGACCTTCAC ACAGATGAGC AGGCTCACAG GCCTCTTGAC 
ACGATAGGCG GTCTCCTGAA CGTCTCATTA CTCTACCACG TCTGGCTGTG TGGCGTCTTC 
CTCTTGGTGA CTTGGTATAG CTCATGGATA CTCTTCAAAA TCTATGCCAC CGAGGCGCAT 
GTGTTCCCTG TTCAGCCGCC TTTTGCAGAA GCCTCAGATG AATGCCTTCC TAAAGTCTTA 
AACAGCAACC CCCCACGCAT CGTAAAGTAT TTGGCCTTAC AGGACCTGAT GCTGCTTTCT 
CAATATTCTC CTTCTCGGAG GCAAGAAGTT TTCAGTCTCA GCCAACCAGG TGGACATCCC 
CACAATTGGA CTGCTATTTC GAGGGAGTGT TTGAATCTTT TAAATGATAT GACTCAGAAA 
CTGGTTCTCT ACCAAGAAGC TGCTGCTACA AATGGGAGGA TGTATTCATC ATACTCAGTG 
GAACCCAAGA AACTGAGCTC TGCAGAAGAA ACTGCTTTTC AGACTCCAAA ACCTAGTCAG 
ACGCCTTCCG TGCCGCCGTT AGTTAAAACT TCACTGTTTT CTCCAAAATT ATCTACACCC 
AATGTTTCAA GTCCTTTTGG GACCCCGTTT GGCTCCAGCG TAGTGAACCG AATGGCTGGA 
ATTCTTGATG TAAACCCCTT CTCTGGGTCT CCCCAAAGTC CTCAGCTCAT AAGAAGAGGA 
CCGAGGCTGT GGACTCACAC TTCTGATCAA CAAGTGTCTG CAATTTCTAA CCCATCTCCT 
TGCGCCTCTG TTACTGCTGA GGGTAAGACA GTGAGACAGC CCAGTGTGAT CTACTCGTGG 
ATTCAGAATA AACGAGAACA GATTAAGAAT TTCTTGTCAA AACGGGTGCT GATAATGTAC 
TTTTTCAGTA AGCACCCAGA GGCCTCCATT CAGGCTGTTT TTTCAGATGC CCAAATGCAT 
ATTTGGGCTT TAGAAGGTCT GTCTCACTTA GTAGCAGCGT CATTTACAGA AGATAGATTT 
GGAGTTGTCC AGACCACACT CCCAGCTATC CTTCATACGC TGTTGACTCT GCAGGAGGCT 
GTCGACAAGT ACTTCAAGCT GCCTCATGCT TCTAGTAAAC CACCCCGAGC TTCAGGAAGC 
CTTGTGGACA CGTCCTACAA AACATTGAGA TTTGCTTTCA GGGCCTCCCT GAAAACCGCT 
ATCTACCGTA TAACCACCAC GTTTGGTGAA CATCTGAACG CTGTGCAGGC ATCTGCAGAA 
CATCAGAAAA GACTTCAGCA ATTCTTGGAG TTCAAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SEPT12-NDC1 Complexes Are Required for Mammalian Spermiogenesis.
International journal of molecular sciences17(11)(2016 Nov)
Lai TH,Wu YY,Wang YY,Chen MF,Wang P,Chen TM,Wu YN,Chiang HS,Kuo PL,Lin YH

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Developmental biology406(1)92-103(2015 Oct)
Lewandowski JP,Du F,Zhang S,Powell MB,Falkenstein KN,Ji H,Vokes SA

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
Genome research25(7)948-57(2015 Jul)
Fairfield H,Srivastava A,Ananda G,Liu R,Kircher M,Lakshminarayana A,Harris BS,Karst SY,Dionne LA,Kane CC,Curtain M,Berry ML,Ward-Bailey PF,Greenstein I,Byers C,Czechanski A,Sharp J,Palmer K,Gudis P,Martin W,Tadenev A,Bogdanik L,Pratt CH,Chang B,Schroeder DG,Cox GA,Cliften P,Milbrandt J,Murray S,Burgess R,Bergstrom DE,Donahue LR,Hamamy H,Masri A,Santoni FA,Makrythanasis P,Antonarakis SE,Shendure J,Reinholdt LG

A mutation in the nuclear pore complex gene Tmem48 causes gametogenesis defects in skeletal fusions with sterility (sks) mice.
The Journal of biological chemistry288(44)31830-41(2013 Nov)
Akiyama K,Noguchi J,Hirose M,Kajita S,Katayama K,Khalaj M,Tsuji T,Fairfield H,Byers C,Reinholdt L,Ogura A,Kunieda T

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Science (New York, N.Y.)328(5978)593-9(2010 Apr)
Hutchins JR,Toyoda Y,Hegemann B,Poser I,H?rich? JK,Sykora MM,Augsburg M,Hudecz O,Buschhorn BA,Bulkescher J,Conrad C,Comartin D,Schleiffer A,Sarov M,Pozniakovsky A,Slabicki MM,Schloissnig S,Steinmacher I,Leuschner M,Ssykor A,Lawo S,Pelletier L,Stark H,Nasmyth K,Ellenberg J,Durbin R,Buchholz F,Mechtler K,Hyman AA,Peters JM

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Ndc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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