Ppme1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppme1
Entrez Gene ID	72590
Full Name	protein phosphatase methylesterase 1
Synonyms	1110069N17Rik,2700017M01Rik,PME-1,Pme1
General protein information	Preferred Names protein phosphatase methylesterase 1 Names protein phosphatase methylesterase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_028292.2	NP_082568.1	protein phosphatase methylesterase 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppme1	NP_001178767.1
Danio rerio (zebrafish)	ppme1	NP_956231.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008336	XP_317121.2
Kluyveromyces lactis	KLLA0D13046g	XP_453644.1
Schizosaccharomyces pombe (fission yeast)	SPBP4H10.17c	NP_596191.1
Magnaporthe oryzae (rice blast fungus)	MGG_15748	XP_003714633.1
Arabidopsis thaliana (thale cress)	AT4G10050	NP_567350.1
Pan troglodytes (chimpanzee)	PPME1	XP_001174909.1
Bos taurus (cattle)	PPME1	NP_001069524.1
Drosophila melanogaster (fruit fly)	CG5068	NP_648277.3
Caenorhabditis elegans (roundworm)	B0464.9	NP_499084.1
Eremothecium gossypii	AGOS_AGR359C	NP_987025.1
Neurospora crassa	NCU00954	XP_965147.1
Xenopus tropicalis (tropical clawed frog)	ppme1	XP_002942566.1
Oryza sativa (rice)	Os04g0443500	NP_001052889.1
Saccharomyces cerevisiae (baker's yeast)	PPE1	NP_011942.1
Homo sapiens (human)	PPME1	NP_057231.1
	PPME1	XP_001115661.1
Canis lupus familiaris (dog)	PPME1	XP_005633576.1
Mus musculus (house mouse)	Ppme1	NP_082568.1
Gallus gallus (chicken)	PPME1	NP_001026005.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation.
Yabe R, Miura A, Usui T, Mudrak I, Ogris E, Ohama T, Sato K
PloS one10(12)e0145226(2015)

Academic cross-fertilization by public screening yields a remarkable class of protein phosphatase methylesterase-1 inhibitors.
Bachovchin DA, Mohr JT, Speers AE, Wang C, Berlin JM, Spicer TP, Fernandez-Vega V, Chase P, Hodder PS, Sch?rer SC, Nomura DK, Rosen H, Fu GC, Cravatt BF
Proceedings of the National Academy of Sciences of the United States of America108(17)6811-6(2011 Apr)

Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.
Sontag JM, Nunbhakdi-Craig V, Mitterhuber M, Ogris E, Sontag E
Journal of neurochemistry115(6)1455-65(2010 Dec)

Targeted disruption of the PME-1 gene causes loss of demethylated PP2A and perinatal lethality in mice.
Ortega-Guti?rrez S, Leung D, Ficarro S, Peters EC, Cravatt BF
PloS one3(7)e2486(2008 Jul)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

protects protein phosphatase 2A from ubiquitin/proteasome degradation
Title: Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation.

Academic cross-fertilization by public screening yields a remarkable class of protein phosphatase methylesterase-1 inhibitors.
Title: Academic cross-fertilization by public screening yields a remarkable class of protein phosphatase methylesterase-1 inhibitors.

PME-1 plays a critical role in differentiation of neuroblastoma cells.
Title: Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.

targeted disruption of the PME-1 gene causes perinatal lethality in mice, a phenotype that correlates with a virtually complete loss of the demethylated form of PP2A in the nervous system and peripheral tissues
Title: Targeted disruption of the PME-1 gene causes loss of demethylated PP2A and perinatal lethality in mice.

The following Ppme1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppme1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05457	NM_028292.2 Latest version!	Mus musculus protein phosphatase methylesterase 1 (Ppme1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppme1 ( NM_028292.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082568.1 Mus musculus protein phosphatase methylesterase 1 (Ppme1), mRNA.

Price & Availability↑

CloneID	OMu05457
Clone ID Related Accession (Same CDS sequence)	NM_028292.2
Accession Version	NM_028292.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein phosphatase methylesterase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC122269.4. On Nov 17, 2006 this sequence version replaced NM_028292.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC014867.1, AK028261.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGCCC TTGAAAAAAG CATGCACCTC GGCCGCCTAC CTTCTCGCCC TCCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGGATGG GCCCTGGACG GAAGCGGGAC 
TTTACCCCTG TCCCATGGAG TCAGTACTTT GAGTCAATGG AAGATGTGGA AGTGGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTTTAC AAGAGTGGTT CTGAGGGTCC AGTCCTGCTG 
CTGCTTCATG GAGGAGGCCA TTCCGCCCTT TCCTGGGCAG TGTTCACGGC AGCAATCATC 
AGTAGAGTAC AGTGTAGGAT TGTGGCTCTG GATCTGCGAG GTCACGGAGA AACTAAAGTC 
AAGAATTCTG AAGACCTGTC AGCAGAAACA ATGGCAAAAG ATGTTGGCAA TGTGGTGGAA 
GCCATGTATG GAGACCTCCC TCCTCCAGTC ATGCTGATTG GGCACAGCAT GGGTGGCGCC 
ATTGCAGTGC ACACAGCCGC AGCTAACCTG GTACCAAGCC TCTTGGGCCT TTGCATGATT 
GATGTTGTAG AAGGTACCGC CATGGATGCA CTTAATAGCA TGCAGAATTT CTTGCGTGGT 
CGACCTAAAA CCTTCAAGTC TTTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAACC TTGAGTCTGC CCGTGTATCC ATGGTCGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGTTC CAAATCCATA GTGGAAGGAA TCATAGAGGA GGAGGAAGAA 
GATGAGGAAG GAAGTGAGTC AGTTAACAAG AGGAAAAAGG AAGACGACAT GGAAACCAAG 
AAGGATCACC CATACACCTG GAGAATTGAG CTGGCAAAAA CAGAAAAGTA CTGGGATGGC 
TGGTTCCGGG GCTTATCCAA TCTCTTTCTT AGCTGTCCTA TTCCTAAACT GCTGCTCTTG 
GCAGGTGTTG ACAGATTGGA TAAAGATCTG ACCATAGGCC AGATGCAGGG GAAGTTCCAG 
ATGCAGGTCT TACCCCAGTG TGGCCATGCA GTCCATGAGG ATGCCCCTGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATCCGGCAC AGGTTTGCAG AGCCCATTGG AGGATTCCAG 
TGTGTGTTTC CTGGCTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082568.1
CDS	74..1234
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFTPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRGHGETKVKNSEDLSAETMAKDVGNVVEAMYGDLPPPVMLIGHSMGGAIAVHTAAANLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESVNKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC

Target ORF information:

RefSeq Version	NM_028292.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase methylesterase 1 (Ppme1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028292.2

ORF Insert Sequence:

ATGTCGGCCC TTGAAAAAAG CATGCACCTC GGCCGCCTAC CTTCTCGCCC TCCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGGATGG GCCCTGGACG GAAGCGGGAC 
TTTACCCCTG TCCCATGGAG TCAGTACTTT GAGTCAATGG AAGATGTGGA AGTGGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTTTAC AAGAGTGGTT CTGAGGGTCC AGTCCTGCTG 
CTGCTTCATG GAGGAGGCCA TTCCGCCCTT TCCTGGGCAG TGTTCACGGC AGCAATCATC 
AGTAGAGTAC AGTGTAGGAT TGTGGCTCTG GATCTGCGAG GTCACGGAGA AACTAAAGTC 
AAGAATTCTG AAGACCTGTC AGCAGAAACA ATGGCAAAAG ATGTTGGCAA TGTGGTGGAA 
GCCATGTATG GAGACCTCCC TCCTCCAGTC ATGCTGATTG GGCACAGCAT GGGTGGCGCC 
ATTGCAGTGC ACACAGCCGC AGCTAACCTG GTACCAAGCC TCTTGGGCCT TTGCATGATT 
GATGTTGTAG AAGGTACCGC CATGGATGCA CTTAATAGCA TGCAGAATTT CTTGCGTGGT 
CGACCTAAAA CCTTCAAGTC TTTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAACC TTGAGTCTGC CCGTGTATCC ATGGTCGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGTTC CAAATCCATA GTGGAAGGAA TCATAGAGGA GGAGGAAGAA 
GATGAGGAAG GAAGTGAGTC AGTTAACAAG AGGAAAAAGG AAGACGACAT GGAAACCAAG 
AAGGATCACC CATACACCTG GAGAATTGAG CTGGCAAAAA CAGAAAAGTA CTGGGATGGC 
TGGTTCCGGG GCTTATCCAA TCTCTTTCTT AGCTGTCCTA TTCCTAAACT GCTGCTCTTG 
GCAGGTGTTG ACAGATTGGA TAAAGATCTG ACCATAGGCC AGATGCAGGG GAAGTTCCAG 
ATGCAGGTCT TACCCCAGTG TGGCCATGCA GTCCATGAGG ATGCCCCTGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATCCGGCAC AGGTTTGCAG AGCCCATTGG AGGATTCCAG 
TGTGTGTTTC CTGGCTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation.
PloS one10(12)e0145226(2015)
Yabe R,Miura A,Usui T,Mudrak I,Ogris E,Ohama T,Sato K

Academic cross-fertilization by public screening yields a remarkable class of protein phosphatase methylesterase-1 inhibitors.
Proceedings of the National Academy of Sciences of the United States of America108(17)6811-6(2011 Apr)
Bachovchin DA,Mohr JT,Speers AE,Wang C,Berlin JM,Spicer TP,Fernandez-Vega V,Chase P,Hodder PS,Sch?rer SC,Nomura DK,Rosen H,Fu GC,Cravatt BF

Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.
Journal of neurochemistry115(6)1455-65(2010 Dec)
Sontag JM,Nunbhakdi-Craig V,Mitterhuber M,Ogris E,Sontag E

Targeted disruption of the PME-1 gene causes loss of demethylated PP2A and perinatal lethality in mice.
PloS one3(7)e2486(2008 Jul)
Ortega-Guti?rrez S,Leung D,Ficarro S,Peters EC,Cravatt BF

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Ppme1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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