Spink5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spink5
Entrez Gene ID	72432
Full Name	serine peptidase inhibitor, Kazal type 5
Synonyms	2310065D10Rik,AV238912,LEKT1,LETK1,VAKT1
General protein information	Preferred Names serine peptidase inhibitor, Kazal type 5 Names serine protease inhibitor Kazal-type 5 Netherton syndrome serine protease inhibitor, Kazal type 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 B3\|18 23.74 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526279.3	XP_006526342.1	serine protease inhibitor Kazal-type 5 isoform X1
XM_006526280.3	XP_006526343.1	serine protease inhibitor Kazal-type 5 isoform X1
XM_006526280.4	XP_006526343.1	serine protease inhibitor Kazal-type 5 isoform X1
NM_001081180.1	NP_001074649.1	serine protease inhibitor Kazal-type 5 precursor
XM_006526279.4	XP_006526342.1	serine protease inhibitor Kazal-type 5 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPINK5	XP_001160618.1
	SPINK5	XP_001103296.1
Rattus norvegicus (Norway rat)	Spink5	NP_001164077.1
Canis lupus familiaris (dog)	SPINK5	NP_001020568.1
Rattus norvegicus (Norway rat)	LOC100910851	XP_003749120.1
Gallus gallus (chicken)	SPINK7	NP_001106132.1
Homo sapiens (human)	SPINK5	NP_001121170.1
Bos taurus (cattle)	SPINK5	NP_001095572.1
Gallus gallus (chicken)	SPINK5	XP_004944964.1
Mus musculus (house mouse)	Spink5	NP_001074649.1

Gene Ontology
Bibliography

Related articles in PubMed

KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype.
Kasparek P, Ileninova Z, Zbodakova O, Kanchev I, Benada O, Chalupsky K, Brattsand M, Beck IM, Sedlacek R
PLoS genetics13(1)e1006566(2017 Jan)

KLK5 Inactivation Reverses Cutaneous Hallmarks of Netherton Syndrome.
Furio L, Pampalakis G, Michael IP, Nagy A, Sotiropoulou G, Hovnanian A
PLoS genetics11(9)e1005389(2015 Sep)

Why we scratch an itch: the molecules, cells and circuits of itch.
Bautista DM, Wilson SR, Hoon MA
Nature neuroscience17(2)175-82(2014 Feb)

Par2 inactivation inhibits early production of TSLP, but not cutaneous inflammation, in Netherton syndrome adult mouse model.
Briot A, Lacroix M, Robin A, Steinhoff M, Deraison C, Hovnanian A
The Journal of investigative dermatology130(12)2736-42(2010 Dec)

Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing.
Bonnart C, Deraison C, Lacroix M, Uchida Y, Besson C, Robin A, Briot A, Gonthier M, Lamant L, Dubus P, Monsarrat B, Hovnanian A
The Journal of clinical investigation120(3)871-82(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spink5/Par2 double knockout (DKO) mice, at embryonic day 19.5, display a dramatic decrease in TSLP expression, although stratum corneum detachment persists, confirming the role of the KLK5-PAR2 cascade in TSLP-mediated early proallergic signaling
Title: Par2 inactivation inhibits early production of TSLP, but not cutaneous inflammation, in Netherton syndrome adult mouse model.

Mouse Spink5/Lekti, like its human counterpart, is involved in the control of epithelial tissue homeostasis.
Title: Characterization and expression analysis of the Spink5 gene, the mouse ortholog of the defective gene in Netherton syndrome.

key regulator of epidermal protease activity
Title: Spink5-deficient mice mimic Netherton syndrome through degradation of desmoglein 1 by epidermal protease hyperactivity.

The following Spink5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spink5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01826	NM_001081180.1 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OMu01826	XM_006526279.4 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu01826	XM_006526280.4 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu01826	XM_006526280.3	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OMu01826	XM_006526279.3	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spink5 ( NM_001081180.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074649.1 Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), mRNA.

Price & Availability↑

CloneID	OMu01826
Clone ID Related Accession (Same CDS sequence)	XM_006526279.3 , XM_006526280.3 , XM_006526280.4 , NM_001081180.1 , XM_006526279.4
Accession Version	NM_001081180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-18
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC138293.11. On or before Jul 19, 2018 this sequence version replaced NM_028237.1, XM_283487.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC118011.1, AJ426449.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074649.1
CDS	66..3119
Translation	MKTATVPMLLTLAFYLTQDAAGEKGNQDPCMKFQAQMKNGTLTCPKGNNSSQSLNDIIFQSECILCKRALEQGAPTKIMNVKVLSRANRATDPAKLNCESFKQRRKDGDFICPSDTSSVCGTDGKTYRSRCELCAENAKSQNHVDVKSEGECGSSHLETDMCSDFRAYVQDGRLGCTRESDPILGPDGRTHGNRCAMCAELFLKEAKENATRNRESRIRRDAEKELCKEFENQVRNGRLFCTRESDPIRGPDGKMHGNKCALCAEIFMRQFTEEKGKAEKNQKDAEERAKAKMEIQKRCSEFQDRARNGTLFCTRENDPIRGLDGKTHGNLCSMCQAFFKTEAEEKKAEAGSRNRRGSEESETYAKLCDEYRKARKNGQLYCTRENAPIRGPDGKIHGNTCSMCQAFFIQEDKARAKVKREAAKEMCSEFRNQARNGMLMCTRENDPVVGPDGKRHSNKCAMCASVFLLEEEEKKKDDKTEKVDAGKAKKEAVQELCRKYHTQLRNGPLRCTRRNNPIEGLDGKMYKNACFMCWAFFQQEAKKSGAGFRPKVKREVKVDCSEYLALSKRGEIFCTRENDPVRGPDGKTHGNKCAMCKAVFKKENEERKRKEGENQRITSGESSSGGNPKAKDECAQYRESMKHGQLSCTRESDPVRGVDGEHYNNKCVMCKELLQKEMEETNKNSASRSNGTGSATGKDVCDQFRSQMKNGKLLCTRESDPTRGPDGAMHGNKCAMCKERLEKEAAEKKKKEDEEKRNTETNKSDKEDKCHEYRSMQLDGRLICTRENDPVRDADGKMHVNKCAMCQMMFEREANERKMREENSRSQPTNEAKDQCGEVHNSVEDAKPRPARSSLPSIRGISKDECSEFQNLMKNEKLTCPETDDPVRGADGTFYQNKCHMCRDVLKNEAMKRSGLQEKSSDIRSTKEGDPEFSSSSRDSDMCKNYRILPRMGYLCPKNLNPVCGDDGQTYSNPCMLCHENLMRQTNTRIHKQGACEESSNLKTVSTGTPASEKMMQ

Target ORF information:

RefSeq Version	NM_001081180.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081180.1

ORF Insert Sequence:

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink5 ( XM_006526279.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526342.1 Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu01826
Clone ID Related Accession (Same CDS sequence)	XM_006526279.3 , XM_006526280.3 , XM_006526280.4 , NM_001081180.1 , XM_006526279.4
Accession Version	XM_006526279.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526279.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526342.1
CDS	466..3519
Translation	MKTATVPMLLTLAFYLTQDAAGEKGNQDPCMKFQAQMKNGTLTCPKGNNSSQSLNDIIFQSECILCKRALEQGAPTKIMNVKVLSRANRATDPAKLNCESFKQRRKDGDFICPSDTSSVCGTDGKTYRSRCELCAENAKSQNHVDVKSEGECGSSHLETDMCSDFRAYVQDGRLGCTRESDPILGPDGRTHGNRCAMCAELFLKEAKENATRNRESRIRRDAEKELCKEFENQVRNGRLFCTRESDPIRGPDGKMHGNKCALCAEIFMRQFTEEKGKAEKNQKDAEERAKAKMEIQKRCSEFQDRARNGTLFCTRENDPIRGLDGKTHGNLCSMCQAFFKTEAEEKKAEAGSRNRRGSEESETYAKLCDEYRKARKNGQLYCTRENAPIRGPDGKIHGNTCSMCQAFFIQEDKARAKVKREAAKEMCSEFRNQARNGMLMCTRENDPVVGPDGKRHSNKCAMCASVFLLEEEEKKKDDKTEKVDAGKAKKEAVQELCRKYHTQLRNGPLRCTRRNNPIEGLDGKMYKNACFMCWAFFQQEAKKSGAGFRPKVKREVKVDCSEYLALSKRGEIFCTRENDPVRGPDGKTHGNKCAMCKAVFKKENEERKRKEGENQRITSGESSSGGNPKAKDECAQYRESMKHGQLSCTRESDPVRGVDGEHYNNKCVMCKELLQKEMEETNKNSASRSNGTGSATGKDVCDQFRSQMKNGKLLCTRESDPTRGPDGAMHGNKCAMCKERLEKEAAEKKKKEDEEKRNTETNKSDKEDKCHEYRSMQLDGRLICTRENDPVRDADGKMHVNKCAMCQMMFEREANERKMREENSRSQPTNEAKDQCGEVHNSVEDAKPRPARSSLPSIRGISKDECSEFQNLMKNEKLTCPETDDPVRGADGTFYQNKCHMCRDVLKNEAMKRSGLQEKSSDIRSTKEGDPEFSSSSRDSDMCKNYRILPRMGYLCPKNLNPVCGDDGQTYSNPCMLCHENLMRQTNTRIHKQGACEESSNLKTVSTGTPASEKMMQ

Target ORF information:

RefSeq Version	XM_006526279.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526279.3

ORF Insert Sequence:

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink5 ( XM_006526280.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526343.1 Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu01826
Clone ID Related Accession (Same CDS sequence)	XM_006526279.3 , XM_006526280.3 , XM_006526280.4 , NM_001081180.1 , XM_006526279.4
Accession Version	XM_006526280.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526343.1
CDS	415..3468
Translation	MKTATVPMLLTLAFYLTQDAAGEKGNQDPCMKFQAQMKNGTLTCPKGNNSSQSLNDIIFQSECILCKRALEQGAPTKIMNVKVLSRANRATDPAKLNCESFKQRRKDGDFICPSDTSSVCGTDGKTYRSRCELCAENAKSQNHVDVKSEGECGSSHLETDMCSDFRAYVQDGRLGCTRESDPILGPDGRTHGNRCAMCAELFLKEAKENATRNRESRIRRDAEKELCKEFENQVRNGRLFCTRESDPIRGPDGKMHGNKCALCAEIFMRQFTEEKGKAEKNQKDAEERAKAKMEIQKRCSEFQDRARNGTLFCTRENDPIRGLDGKTHGNLCSMCQAFFKTEAEEKKAEAGSRNRRGSEESETYAKLCDEYRKARKNGQLYCTRENAPIRGPDGKIHGNTCSMCQAFFIQEDKARAKVKREAAKEMCSEFRNQARNGMLMCTRENDPVVGPDGKRHSNKCAMCASVFLLEEEEKKKDDKTEKVDAGKAKKEAVQELCRKYHTQLRNGPLRCTRRNNPIEGLDGKMYKNACFMCWAFFQQEAKKSGAGFRPKVKREVKVDCSEYLALSKRGEIFCTRENDPVRGPDGKTHGNKCAMCKAVFKKENEERKRKEGENQRITSGESSSGGNPKAKDECAQYRESMKHGQLSCTRESDPVRGVDGEHYNNKCVMCKELLQKEMEETNKNSASRSNGTGSATGKDVCDQFRSQMKNGKLLCTRESDPTRGPDGAMHGNKCAMCKERLEKEAAEKKKKEDEEKRNTETNKSDKEDKCHEYRSMQLDGRLICTRENDPVRDADGKMHVNKCAMCQMMFEREANERKMREENSRSQPTNEAKDQCGEVHNSVEDAKPRPARSSLPSIRGISKDECSEFQNLMKNEKLTCPETDDPVRGADGTFYQNKCHMCRDVLKNEAMKRSGLQEKSSDIRSTKEGDPEFSSSSRDSDMCKNYRILPRMGYLCPKNLNPVCGDDGQTYSNPCMLCHENLMRQTNTRIHKQGACEESSNLKTVSTGTPASEKMMQ

Target ORF information:

RefSeq Version	XM_006526280.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526280.3

ORF Insert Sequence:

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink5 ( XM_006526280.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526343.1 Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu01826
Clone ID Related Accession (Same CDS sequence)	XM_006526279.3 , XM_006526280.3 , XM_006526280.4 , NM_001081180.1 , XM_006526279.4
Accession Version	XM_006526280.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526280.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526343.1
CDS	415..3468
Translation	MKTATVPMLLTLAFYLTQDAAGEKGNQDPCMKFQAQMKNGTLTCPKGNNSSQSLNDIIFQSECILCKRALEQGAPTKIMNVKVLSRANRATDPAKLNCESFKQRRKDGDFICPSDTSSVCGTDGKTYRSRCELCAENAKSQNHVDVKSEGECGSSHLETDMCSDFRAYVQDGRLGCTRESDPILGPDGRTHGNRCAMCAELFLKEAKENATRNRESRIRRDAEKELCKEFENQVRNGRLFCTRESDPIRGPDGKMHGNKCALCAEIFMRQFTEEKGKAEKNQKDAEERAKAKMEIQKRCSEFQDRARNGTLFCTRENDPIRGLDGKTHGNLCSMCQAFFKTEAEEKKAEAGSRNRRGSEESETYAKLCDEYRKARKNGQLYCTRENAPIRGPDGKIHGNTCSMCQAFFIQEDKARAKVKREAAKEMCSEFRNQARNGMLMCTRENDPVVGPDGKRHSNKCAMCASVFLLEEEEKKKDDKTEKVDAGKAKKEAVQELCRKYHTQLRNGPLRCTRRNNPIEGLDGKMYKNACFMCWAFFQQEAKKSGAGFRPKVKREVKVDCSEYLALSKRGEIFCTRENDPVRGPDGKTHGNKCAMCKAVFKKENEERKRKEGENQRITSGESSSGGNPKAKDECAQYRESMKHGQLSCTRESDPVRGVDGEHYNNKCVMCKELLQKEMEETNKNSASRSNGTGSATGKDVCDQFRSQMKNGKLLCTRESDPTRGPDGAMHGNKCAMCKERLEKEAAEKKKKEDEEKRNTETNKSDKEDKCHEYRSMQLDGRLICTRENDPVRDADGKMHVNKCAMCQMMFEREANERKMREENSRSQPTNEAKDQCGEVHNSVEDAKPRPARSSLPSIRGISKDECSEFQNLMKNEKLTCPETDDPVRGADGTFYQNKCHMCRDVLKNEAMKRSGLQEKSSDIRSTKEGDPEFSSSSRDSDMCKNYRILPRMGYLCPKNLNPVCGDDGQTYSNPCMLCHENLMRQTNTRIHKQGACEESSNLKTVSTGTPASEKMMQ

Target ORF information:

RefSeq Version	XM_006526280.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526280.4

ORF Insert Sequence:

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink5 ( XM_006526279.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526342.1 Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X1, mRNA.

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CloneID	OMu01826
Clone ID Related Accession (Same CDS sequence)	XM_006526279.3 , XM_006526280.3 , XM_006526280.4 , NM_001081180.1 , XM_006526279.4
Accession Version	XM_006526279.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526279.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526342.1
CDS	466..3519
Translation	MKTATVPMLLTLAFYLTQDAAGEKGNQDPCMKFQAQMKNGTLTCPKGNNSSQSLNDIIFQSECILCKRALEQGAPTKIMNVKVLSRANRATDPAKLNCESFKQRRKDGDFICPSDTSSVCGTDGKTYRSRCELCAENAKSQNHVDVKSEGECGSSHLETDMCSDFRAYVQDGRLGCTRESDPILGPDGRTHGNRCAMCAELFLKEAKENATRNRESRIRRDAEKELCKEFENQVRNGRLFCTRESDPIRGPDGKMHGNKCALCAEIFMRQFTEEKGKAEKNQKDAEERAKAKMEIQKRCSEFQDRARNGTLFCTRENDPIRGLDGKTHGNLCSMCQAFFKTEAEEKKAEAGSRNRRGSEESETYAKLCDEYRKARKNGQLYCTRENAPIRGPDGKIHGNTCSMCQAFFIQEDKARAKVKREAAKEMCSEFRNQARNGMLMCTRENDPVVGPDGKRHSNKCAMCASVFLLEEEEKKKDDKTEKVDAGKAKKEAVQELCRKYHTQLRNGPLRCTRRNNPIEGLDGKMYKNACFMCWAFFQQEAKKSGAGFRPKVKREVKVDCSEYLALSKRGEIFCTRENDPVRGPDGKTHGNKCAMCKAVFKKENEERKRKEGENQRITSGESSSGGNPKAKDECAQYRESMKHGQLSCTRESDPVRGVDGEHYNNKCVMCKELLQKEMEETNKNSASRSNGTGSATGKDVCDQFRSQMKNGKLLCTRESDPTRGPDGAMHGNKCAMCKERLEKEAAEKKKKEDEEKRNTETNKSDKEDKCHEYRSMQLDGRLICTRENDPVRDADGKMHVNKCAMCQMMFEREANERKMREENSRSQPTNEAKDQCGEVHNSVEDAKPRPARSSLPSIRGISKDECSEFQNLMKNEKLTCPETDDPVRGADGTFYQNKCHMCRDVLKNEAMKRSGLQEKSSDIRSTKEGDPEFSSSSRDSDMCKNYRILPRMGYLCPKNLNPVCGDDGQTYSNPCMLCHENLMRQTNTRIHKQGACEESSNLKTVSTGTPASEKMMQ

Target ORF information:

RefSeq Version	XM_006526279.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526279.4

ORF Insert Sequence:

ATGAAGACAG CCACGGTGCC AATGCTTCTG ACCCTGGCAT TTTATCTCAC ACAAGATGCT 
GCTGGTGAGA AAGGCAATCA GGACCCATGC ATGAAATTTC AAGCACAGAT GAAAAATGGT 
ACACTGACGT GTCCCAAAGG CAACAATTCT TCTCAAAGTC TCAATGACAT CATTTTCCAA 
AGTGAATGCA TCTTGTGCAA AAGAGCACTA GAACAAGGAG CACCTACCAA GATCATGAAT 
GTGAAGGTTT TGTCAAGAGC CAACAGGGCC ACTGACCCAG CAAAGTTGAA CTGTGAAAGT 
TTCAAACAAA GAAGAAAAGA TGGGGATTTC ATCTGTCCAT CTGACACTTC TTCAGTTTGT 
GGCACAGATG GGAAAACATA CCGCAGTAGA TGTGAACTGT GTGCTGAGAA TGCGAAAAGC 
CAGAACCATG TTGACGTAAA GAGTGAAGGT GAATGTGGCA GCAGCCACCT AGAGACGGAT 
ATGTGCAGTG ATTTTCGAGC CTATGTGCAA GATGGAAGAC TTGGATGTAC ACGGGAAAGT 
GATCCAATCC TTGGTCCTGA TGGGAGGACA CATGGCAATA GGTGTGCCAT GTGTGCTGAG 
CTCTTTTTAA AAGAAGCTAA AGAAAATGCC ACTCGTAACA GAGAGAGCAG AATTCGGAGA 
GATGCCGAGA AGGAACTTTG CAAGGAATTT GAAAACCAAG TGAGGAATGG AAGACTCTTC 
TGTACCCGGG AAAGTGACCC AATCCGTGGC CCTGATGGCA AGATGCATGG GAACAAGTGT 
GCCCTCTGTG CTGAGATATT CATGAGGCAA TTTACAGAAG AAAAAGGTAA AGCAGAGAAA 
AACCAGAAAG ATGCTGAAGA AAGGGCTAAA GCTAAAATGG AAATTCAGAA ACGATGTAGT 
GAGTTCCAGG ATCGTGCAAG GAATGGAACG CTGTTCTGTA CCAGAGAAAA TGATCCTATT 
CGTGGTTTGG ATGGAAAAAC ACATGGCAAC TTGTGTTCCA TGTGCCAGGC TTTCTTCAAA 
ACAGAAGCTG AAGAAAAGAA GGCTGAAGCT GGAAGCAGAA ACAGAAGAGG GTCTGAAGAG 
TCAGAAACAT ATGCAAAGCT ATGTGATGAA TATCGCAAGG CCAGGAAGAA TGGACAACTA 
TATTGCACCA GAGAGAATGC CCCAATCAGG GGCCCTGATG GGAAAATCCA TGGCAACACA 
TGCTCCATGT GTCAGGCTTT CTTTATTCAA GAAGACAAAG CAAGAGCAAA GGTTAAAAGA 
GAAGCTGCAA AGGAAATGTG CAGTGAGTTC CGGAACCAAG CAAGAAATGG AATGCTCATG 
TGCACCAGAG AAAATGACCC TGTTGTTGGG CCAGATGGAA AAAGACATTC AAACAAATGT 
GCAATGTGTG CAAGTGTATT CCTACTAGAA GAGGAAGAGA AGAAAAAAGA TGACAAAACG 
GAGAAGGTTG ACGCTGGAAA GGCGAAGAAA GAAGCTGTAC AGGAGCTCTG TCGTAAATAC 
CATACCCAGC TCAGAAATGG GCCGCTCCGC TGCACCAGAA GGAATAACCC CATTGAGGGC 
CTGGATGGGA AGATGTATAA AAATGCCTGC TTCATGTGTT GGGCCTTCTT CCAGCAAGAA 
GCCAAGAAAA GTGGGGCTGG TTTCAGACCA AAGGTTAAAA GAGAAGTTAA GGTTGACTGC 
AGTGAATATC TGGCTCTTTC AAAACGTGGA GAAATTTTCT GCACACGAGA AAATGACCCT 
GTGCGTGGCC CAGATGGCAA AACCCATGGC AACAAGTGTG CCATGTGCAA GGCCGTCTTC 
AAGAAAGAAA ATGAAGAAAG AAAGAGGAAA GAAGGGGAAA ATCAGAGAAT CACCTCAGGG 
GAGAGTTCCA GTGGTGGGAA CCCGAAGGCT AAGGATGAAT GTGCTCAGTA TCGGGAAAGC 
ATGAAACATG GACAACTCAG CTGTACTAGG GAGAGTGACC CTGTACGTGG TGTCGATGGG 
GAACACTACA ACAATAAATG TGTCATGTGT AAAGAACTAC TGCAGAAAGA AATGGAGGAA 
ACAAATAAAA ATTCTGCCTC AAGATCGAAT GGAACTGGAT CAGCAACAGG AAAGGATGTA 
TGTGATCAGT TTAGAAGCCA AATGAAAAAT GGGAAACTCC TCTGCACTCG GGAAAGTGAC 
CCTACCCGGG GTCCGGATGG CGCAATGCAT GGCAACAAGT GTGCCATGTG TAAGGAAAGA 
CTGGAAAAAG AAGCTGCTGA AAAGAAAAAG AAAGAGGATG AAGAGAAGAG AAACACTGAA 
ACAAACAAGA GCGATAAGGA GGATAAATGC CATGAATACC GGAGCATGCA GCTAGATGGA 
AGGCTGATCT GCACCAGAGA AAATGATCCC GTTCGAGACG CAGATGGCAA GATGCATGTT 
AACAAGTGTG CTATGTGTCA GATGATGTTT GAGCGTGAAG CTAATGAAAG AAAAATGAGA 
GAAGAAAACT CACGCTCCCA GCCCACAAAT GAAGCAAAGG ACCAGTGTGG AGAAGTTCAT 
AACTCAGTAG AGGATGCCAA ACCGAGGCCA GCCAGGAGTT CTTTGCCCTC CATCCGTGGG 
ATCAGTAAAG ATGAGTGCAG TGAGTTTCAA AATCTCATGA AGAATGAAAA ACTTACCTGT 
CCAGAAACAG ATGACCCGGT GCGTGGTGCT GATGGAACCT TTTATCAGAA CAAGTGCCAC 
ATGTGCAGAG ATGTCCTTAA AAATGAAGCT ATGAAAAGGT CTGGGCTTCA AGAAAAGTCA 
TCAGACATTA GGTCTACTAA AGAAGGTGAT CCAGAATTTT CCAGCTCCTC TAGGGACTCT 
GACATGTGCA AAAACTACCG AATATTACCC AGAATGGGTT ATCTTTGCCC AAAGAATCTA 
AACCCTGTCT GTGGTGATGA TGGCCAAACA TACTCCAACC CTTGTATGCT CTGTCATGAA 
AACCTGATGC GTCAGACTAA TACACGCATA CATAAACAAG GAGCATGTGA GGAAAGCAGC 
AACCTGAAAA CAGTATCTAC TGGCACACCT GCATCTGAAA AAATGATGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype.
PLoS genetics13(1)e1006566(2017 Jan)
Kasparek P,Ileninova Z,Zbodakova O,Kanchev I,Benada O,Chalupsky K,Brattsand M,Beck IM,Sedlacek R

KLK5 Inactivation Reverses Cutaneous Hallmarks of Netherton Syndrome.
PLoS genetics11(9)e1005389(2015 Sep)
Furio L,Pampalakis G,Michael IP,Nagy A,Sotiropoulou G,Hovnanian A

Why we scratch an itch: the molecules, cells and circuits of itch.
Nature neuroscience17(2)175-82(2014 Feb)
Bautista DM,Wilson SR,Hoon MA

Par2 inactivation inhibits early production of TSLP, but not cutaneous inflammation, in Netherton syndrome adult mouse model.
The Journal of investigative dermatology130(12)2736-42(2010 Dec)
Briot A,Lacroix M,Robin A,Steinhoff M,Deraison C,Hovnanian A

Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing.
The Journal of clinical investigation120(3)871-82(2010 Mar)
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Spink5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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