Chst14 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chst14
Entrez Gene ID	72136
Full Name	carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14
Synonyms	2600016L03Rik,D4ST-1,D4st1
General protein information	Preferred Names carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 Names carbohydrate sulfotransferase 14 dermatan 4-sulfotransferase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_028117.3	NP_082393.3	carbohydrate sulfotransferase 14

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHST14	XP_510310.2
	CHST14	XP_001096685.1
Xenopus tropicalis (tropical clawed frog)	chst14	XP_004917361.1
Homo sapiens (human)	CHST14	NP_569735.1
Canis lupus familiaris (dog)	CHST14	XP_005638734.1
Rattus norvegicus (Norway rat)	Chst14	NP_001103109.1
Mus musculus (house mouse)	Chst14	NP_082393.3
Gallus gallus (chicken)	CHST14	XP_003641334.2
Bos taurus (cattle)	CHST14	NP_001091481.1
Danio rerio (zebrafish)	chst14	NP_997994.1

Gene Ontology
Bibliography

Related articles in PubMed

Vascular abnormalities in the placenta of Chst14-/- fetuses: implications in the pathophysiology of perinatal lethality of the murine model and vascular lesions in human CHST14/D4ST1 deficiency.
Yoshizawa T, Mizumoto S, Takahashi Y, Shimada S, Sugahara K, Nakayama J, Takeda S, Nomura Y, Nitahara-Kasahara Y, Okada T, Matsumoto K, Yamada S, Kosho T
Glycobiology28(2)80-89(2018 Feb)

Germline ablation of dermatan-4O-sulfotransferase1 reduces regeneration after mouse spinal cord injury.
Rost S, Aky?z N, Martinovic T, Huckhagel T, Jakovcevski I, Schachner M
Neuroscience31274-85(2016 Jan)

Dermatan 4-O-sulfotransferase1 ablation accelerates peripheral nerve regeneration.
Aky?z N, Rost S, Mehanna A, Bian S, Loers G, Oezen I, Mishra B, Hoffmann K, Guseva D, Laczynska E, Irintchev A, Jakovcevski I, Schachner M
Experimental neurology247517-30(2013 Sep)

Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells.
Bian S, Aky?z N, Bernreuther C, Loers G, Laczynska E, Jakovcevski I, Schachner M
Journal of cell science124(Pt 23)4051-63(2011 Dec)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deficiency for Chst14 results in decreased neurogenesis and diminished proliferation of neural stem cells.
Title: Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells.

analysis of the three CS sulfotransferases in the developing neocortex: uronyl 2-O-sulfotransferase (UST), N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase (4,6-ST) and dermatan 4-O-sulfotransferase-1 (D4-ST)
Title: Oversulfated chondroitin sulfate plays critical roles in the neuronal migration in the cerebral cortex.

The following Chst14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chst14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58556	NM_028117.3 Latest version!	Mus musculus carbohydrate sulfotransferase 14 (Chst14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chst14 ( NM_028117.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082393.3 Mus musculus carbohydrate sulfotransferase 14 (Chst14), mRNA.

Price & Availability↑

CloneID	OMu58556
Clone ID Related Accession (Same CDS sequence)	NM_028117.3
Accession Version	NM_028117.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	carbohydrate sulfotransferase 14
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845164.5. On Jul 21, 2009 this sequence version replaced NM_028117.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript is intronless :: AK154555.1 [ECO:0000345] ##Evidence-Data-END##

ATGTTTCCCC GCCCTCTGAC CCCACTGGCT GCCCCGAAAA GCGCGGAGAC CCTGGGCCGC 
ACGCCAAGGC GGGCCCCATT GGGCCGGGCC CGGGCTGGGC TCGGGGGGCC GCCCCTGCTG 
CTGCCGTCCA TGCTGATGTT CGCTGTAATC GTGGCCTCCA GCGGACTGCT GCTCATGATC 
GAGCGAGGCA TCCTATCGGA GATGAAACCC CTTCCCCTGC ACCCTCCCAG CCACAAAGGC 
GCGGCCTGGA GCGGGACAGA TCCTAAGCCT AGAGGCCTAT CCTTGGATGC TGGGGACTCG 
GACTTGCAAG TGAGGGAGGA CATCCGAAAC CGGACCTTGA GGGCCGTGTG CGGACAACCA 
GGCATGCCCC GGGACCCCTG GGACTTGCCG GTGGGACAGC GGCGCACCCT GCTGCGCCAC 
ATTCTCGTAA GTGACCGCTA CCGCTTCCTC TACTGCTATG TCCCCAAAGT GGCCTGCTCT 
AACTGGAAAC GTGTGCTGAA GGTGCTGGCT GGCATCCTGA ACAACGTGGA TGTCCGCCTC 
AAGATGGACC ACCGCAGTGA CTTGGTGTTT CTGGCAGACC TGCGGCCTGA GGAGATTCGC 
TACCGTCTGC AGCACTACTT CAAGTTCCTG TTTGTGCGAG ACCCCTTGGA ACGCCTCCTG 
TCTGCTTACC GTAACAAGTT TGGAGAGATC CGAGAGTACC AGCAGCGATA TGGGGCCGAA 
ATTGTCAGGC GCTACAGGGC TGGAGCTGGC CCCAGCCCTG CAGGGGACGA TGTCACCTTC 
CCAGAGTTCC TGAGATACCT GGTGGATGAG GATCCTGAAC ATATGAATGA GCATTGGATG 
CCTGTGTACC ACCTGTGCCA ACCATGTGCT GTGCACTACG ACTTCGTGGG TTCCTATGAG 
AGGCTGGAGG CTGATGCCAA CCAGGTGCTG GAGTGGGTGC GGGCCCCACC CCATGTCCGG 
TTCCCAGCTC GCCAGGCCTG GTACCGGCCA GCCAGCCCAG AAAGTCTGCA TTACCACTTG 
TGCAATGTTC CACGGGCCCT GCTTCAAGAT GTGCTACCTA AGTATATCCT GGACTTCTCC 
CTCTTTGCTT ACCCACTGCC CAATGTCACC AAGGAAGCCT GTCACCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082393.3
CDS	156..1286
Translation	MFPRPLTPLAAPKSAETLGRTPRRAPLGRARAGLGGPPLLLPSMLMFAVIVASSGLLLMIERGILSEMKPLPLHPPSHKGAAWSGTDPKPRGLSLDAGDSDLQVREDIRNRTLRAVCGQPGMPRDPWDLPVGQRRTLLRHILVSDRYRFLYCYVPKVACSNWKRVLKVLAGILNNVDVRLKMDHRSDLVFLADLRPEEIRYRLQHYFKFLFVRDPLERLLSAYRNKFGEIREYQQRYGAEIVRRYRAGAGPSPAGDDVTFPEFLRYLVDEDPEHMNEHWMPVYHLCQPCAVHYDFVGSYERLEADANQVLEWVRAPPHVRFPARQAWYRPASPESLHYHLCNVPRALLQDVLPKYILDFSLFAYPLPNVTKEACHQ

Target ORF information:

RefSeq Version	NM_028117.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbohydrate sulfotransferase 14 (Chst14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028117.3

ORF Insert Sequence:

ATGTTTCCCC GCCCTCTGAC CCCACTGGCT GCCCCGAAAA GCGCGGAGAC CCTGGGCCGC 
ACGCCAAGGC GGGCCCCATT GGGCCGGGCC CGGGCTGGGC TCGGGGGGCC GCCCCTGCTG 
CTGCCGTCCA TGCTGATGTT CGCTGTAATC GTGGCCTCCA GCGGACTGCT GCTCATGATC 
GAGCGAGGCA TCCTATCGGA GATGAAACCC CTTCCCCTGC ACCCTCCCAG CCACAAAGGC 
GCGGCCTGGA GCGGGACAGA TCCTAAGCCT AGAGGCCTAT CCTTGGATGC TGGGGACTCG 
GACTTGCAAG TGAGGGAGGA CATCCGAAAC CGGACCTTGA GGGCCGTGTG CGGACAACCA 
GGCATGCCCC GGGACCCCTG GGACTTGCCG GTGGGACAGC GGCGCACCCT GCTGCGCCAC 
ATTCTCGTAA GTGACCGCTA CCGCTTCCTC TACTGCTATG TCCCCAAAGT GGCCTGCTCT 
AACTGGAAAC GTGTGCTGAA GGTGCTGGCT GGCATCCTGA ACAACGTGGA TGTCCGCCTC 
AAGATGGACC ACCGCAGTGA CTTGGTGTTT CTGGCAGACC TGCGGCCTGA GGAGATTCGC 
TACCGTCTGC AGCACTACTT CAAGTTCCTG TTTGTGCGAG ACCCCTTGGA ACGCCTCCTG 
TCTGCTTACC GTAACAAGTT TGGAGAGATC CGAGAGTACC AGCAGCGATA TGGGGCCGAA 
ATTGTCAGGC GCTACAGGGC TGGAGCTGGC CCCAGCCCTG CAGGGGACGA TGTCACCTTC 
CCAGAGTTCC TGAGATACCT GGTGGATGAG GATCCTGAAC ATATGAATGA GCATTGGATG 
CCTGTGTACC ACCTGTGCCA ACCATGTGCT GTGCACTACG ACTTCGTGGG TTCCTATGAG 
AGGCTGGAGG CTGATGCCAA CCAGGTGCTG GAGTGGGTGC GGGCCCCACC CCATGTCCGG 
TTCCCAGCTC GCCAGGCCTG GTACCGGCCA GCCAGCCCAG AAAGTCTGCA TTACCACTTG 
TGCAATGTTC CACGGGCCCT GCTTCAAGAT GTGCTACCTA AGTATATCCT GGACTTCTCC 
CTCTTTGCTT ACCCACTGCC CAATGTCACC AAGGAAGCCT GTCACCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vascular abnormalities in the placenta of Chst14-/- fetuses: implications in the pathophysiology of perinatal lethality of the murine model and vascular lesions in human CHST14/D4ST1 deficiency.
Glycobiology28(2)80-89(2018 Feb)
Yoshizawa T,Mizumoto S,Takahashi Y,Shimada S,Sugahara K,Nakayama J,Takeda S,Nomura Y,Nitahara-Kasahara Y,Okada T,Matsumoto K,Yamada S,Kosho T

Germline ablation of dermatan-4O-sulfotransferase1 reduces regeneration after mouse spinal cord injury.
Neuroscience31274-85(2016 Jan)
Rost S,Aky?z N,Martinovic T,Huckhagel T,Jakovcevski I,Schachner M

Dermatan 4-O-sulfotransferase1 ablation accelerates peripheral nerve regeneration.
Experimental neurology247517-30(2013 Sep)
Aky?z N,Rost S,Mehanna A,Bian S,Loers G,Oezen I,Mishra B,Hoffmann K,Guseva D,Laczynska E,Irintchev A,Jakovcevski I,Schachner M

Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells.
Journal of cell science124(Pt 23)4051-63(2011 Dec)
Bian S,Aky?z N,Bernreuther C,Loers G,Laczynska E,Jakovcevski I,Schachner M

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

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Chst14 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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