Sars2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sars2
Entrez Gene ID	71984
Full Name	seryl-aminoacyl-tRNA synthetase 2
Synonyms	2410015F05Rik,D7Ertd353e,SerRS,SerRSmt
General protein information	Preferred Names seryl-aminoacyl-tRNA synthetase 2 Names serine--tRNA ligase, mitochondrial seryl-tRNA synthetase, mitochondrial seryl-tRNA(Ser/Sec) synthetase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B1\|7 16.91 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_023637.3	NP_076126.2	serine--tRNA ligase, mitochondrial precursor
XM_011250711.2	XP_011249013.1	serine--tRNA ligase, mitochondrial isoform X2
XM_006540376.2	XP_006540439.1	serine--tRNA ligase, mitochondrial isoform X2
XM_006540375.3	XP_006540438.1	serine--tRNA ligase, mitochondrial isoform X1
XM_006540375.4	XP_006540438.1	serine--tRNA ligase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sars2	NP_001099710.1
Schizosaccharomyces pombe (fission yeast)	SPAC25B8.06c	NP_594466.2
Arabidopsis thaliana (thale cress)	SRS	NP_849647.1
Pan troglodytes (chimpanzee)	LOC748729	XP_001162887.2
Bos taurus (cattle)	SARS2	NP_776882.1
Eremothecium gossypii	AGOS_AFR412C	NP_985959.1
Neurospora crassa	NCU09594	XP_963172.2
	SARS2	XP_001085926.2
Canis lupus familiaris (dog)	SARS2	XP_005616944.1
Drosophila melanogaster (fruit fly)	Aats-ser	NP_524365.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000991	XP_001688169.2
Kluyveromyces lactis	KLLA0E03433g	XP_454099.1
Xenopus tropicalis (tropical clawed frog)	sars2	XP_002940400.1
Homo sapiens (human)	SARS2	NP_060297.1
Caenorhabditis elegans (roundworm)	sars-2	NP_500549.1
Saccharomyces cerevisiae (baker's yeast)	DIA4	NP_011875.1
Magnaporthe oryzae (rice blast fungus)	MGG_03230	XP_003716749.1
Oryza sativa (rice)	Os11g0610900	NP_001068265.1
Mus musculus (house mouse)	Sars2	NP_076126.2
Danio rerio (zebrafish)	sars2	NP_957473.1

Gene Ontology
Bibliography

Related articles in PubMed

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Gineste C, De Winter JM, Kohl C, Witt CC, Giannesini B, Brohm K, Le Fur Y, Gretz N, Vilmen C, Pecchi E, Jubeau M, Cozzone PJ, Stienen GJ, Granzier H, Labeit S, Ottenheijm CA, Bendahan D, Gondin J
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)

The bidirectional promoter of two genes for the mitochondrial translational apparatus in mouse is regulated by an array of CCAAT boxes interacting with the transcription factor NF-Y.
Zanotto E, Shah ZH, Jacobs HT
Nucleic acids research35(2)664-77(2007)

Genomic organization, expression, and subcellular localization of mouse mitochondrial seryl-tRNA synthetase.
Gibbons WJ Jr, Yan Q, Li R, Li X, Guan MX
Biochemical and biophysical research communications317(3)774-8(2004 May)

Characterization and tRNA recognition of mammalian mitochondrial seryl-tRNA synthetase.
Yokogawa T, Shimada N, Takeuchi N, Benkowski L, Suzuki T, Omori A, Ueda T, Nishikawa K, Spremulli LL, Watanabe K
The Journal of biological chemistry275(26)19913-20(2000 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identification of an array of 4 CCAAT box elements required for efficient transcription of the Sars2 gene.
Title: The bidirectional promoter of two genes for the mitochondrial translational apparatus in mouse is regulated by an array of CCAAT boxes interacting with the transcription factor NF-Y.

The following Sars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38510	XM_006540376.2 Latest version!	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu70810	XM_011250711.2 Latest version!	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu38509	XM_006540375.4 Latest version!	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu15593	NM_023637.3 Latest version!	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$300.30 $429.00
View Old Accession Versions >>
OMu38509	XM_006540375.3	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sars2 ( XM_006540376.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540439.1 Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu38510
Clone ID Related Accession (Same CDS sequence)	XM_006540376.2
Accession Version	XM_006540376.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006540376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTCC CAGACCTTCT GAGAGGAGCT GTGTTTGAAG GCTGTGGAAT GACACCAAAT 
GCCAACCCAT CCCAGATTTA CAATATTGAC CCCTCTCGCT TCGAAGACCT TAACCTGGCC 
GGGACGGCAG AGGTGGGGCT GGCAGGATAC TTCATGGACC ACTCTGTAGC TTTCAGGGAC 
CTTCCAGTCA GGATGGTCTG TGCCAGCACC TGCTACCGGG CAGAGACAGA CACTGGGAAG 
GAGCCTTGGG GACTGTACCG AGTCCATCAC TTCACTAAGG TGGAGATGTT TGGGGTGACA 
GGCCCTGGGC TGGAGCAGAG CTCGCAGCTG TTGGATGAGT TCCTGTCTCT GCAGGTGGAG 
ATCTTGACGG AGCTGGGCTT GCACTTCCGG GTACTGGACA TGCCCACCCA GGAACTGGGC 
CTTCCTGCTT ATCGCAAGTT CGACATTGAG GCCTGGATGC CTGGCCGAGG CCGCTATGGA 
GAGGTCACCA GTGCCTCCAA CTGCACAGAC TTCCAGAGCC GCCGCCTGTA CATCATGTTT 
GAGACAGAGA CCGGGGAGCT GCAGTTCGCA CACACGGTGA ATGCCACCGC TTGTGCTGTT 
CCCCGAGTCC TCATTGCCCT CCTGGAAAGC AATCAGCAAA AGGATGGTTC AGTCCTCGTG 
CCCGCCGCTC TCCAGCCCTA CCTAGGCACG GACCGGATCA CAGCCCCTAC CCATGTGCCT 
CTCCAGTACA TCGGCCCCAA TCAGCCTCAG AAGCCCAGGC TCCCCGGCCA GTCTGCCACC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540439.1
CDS	76..861
Translation	MTVPDLLRGAVFEGCGMTPNANPSQIYNIDPSRFEDLNLAGTAEVGLAGYFMDHSVAFRDLPVRMVCASTCYRAETDTGKEPWGLYRVHHFTKVEMFGVTGPGLEQSSQLLDEFLSLQVEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRYGEVTSASNCTDFQSRRLYIMFETETGELQFAHTVNATACAVPRVLIALLESNQQKDGSVLVPAALQPYLGTDRITAPTHVPLQYIGPNQPQKPRLPGQSATR

Target ORF information:

RefSeq Version	XM_006540376.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540376.2

ORF Insert Sequence:

ATGACGGTCC CAGACCTTCT GAGAGGAGCT GTGTTTGAAG GCTGTGGAAT GACACCAAAT 
GCCAACCCAT CCCAGATTTA CAATATTGAC CCCTCTCGCT TCGAAGACCT TAACCTGGCC 
GGGACGGCAG AGGTGGGGCT GGCAGGATAC TTCATGGACC ACTCTGTAGC TTTCAGGGAC 
CTTCCAGTCA GGATGGTCTG TGCCAGCACC TGCTACCGGG CAGAGACAGA CACTGGGAAG 
GAGCCTTGGG GACTGTACCG AGTCCATCAC TTCACTAAGG TGGAGATGTT TGGGGTGACA 
GGCCCTGGGC TGGAGCAGAG CTCGCAGCTG TTGGATGAGT TCCTGTCTCT GCAGGTGGAG 
ATCTTGACGG AGCTGGGCTT GCACTTCCGG GTACTGGACA TGCCCACCCA GGAACTGGGC 
CTTCCTGCTT ATCGCAAGTT CGACATTGAG GCCTGGATGC CTGGCCGAGG CCGCTATGGA 
GAGGTCACCA GTGCCTCCAA CTGCACAGAC TTCCAGAGCC GCCGCCTGTA CATCATGTTT 
GAGACAGAGA CCGGGGAGCT GCAGTTCGCA CACACGGTGA ATGCCACCGC TTGTGCTGTT 
CCCCGAGTCC TCATTGCCCT CCTGGAAAGC AATCAGCAAA AGGATGGTTC AGTCCTCGTG 
CCCGCCGCTC TCCAGCCCTA CCTAGGCACG GACCGGATCA CAGCCCCTAC CCATGTGCCT 
CTCCAGTACA TCGGCCCCAA TCAGCCTCAG AAGCCCAGGC TCCCCGGCCA GTCTGCCACC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sars2 ( XM_006540375.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540438.1 Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38509
Clone ID Related Accession (Same CDS sequence)	XM_006540375.3 , XM_006540375.4
Accession Version	XM_006540375.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540375.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CCATGGCGCG GCTTTGGTGG CCTTTCCTGG CTCGCCAGGG GCTCCGGTCC 
CGAGGACGCT GCGTGTGCAG CCAGAACCCA AGGAGGAGTT TCGCTACGGA GAAACGAGTC 
CGGAACCTCC TGTACGAACA CGCACGAGAG GGCTACAGCG AGCTTCCCTA CTTGGACATG 
GAGTCGGTGT GCGCATGCCC AGAAAAAGCC GCGCGCTCCC TGGAGCTCCG CAAGGGGGAG 
CTGCGGCCGG CCGACCTGCC TGCGATTATC TCCACGTGGC AGGAGTTGAG GCAGCTTCGA 
GAGCAGATCC GGAGCCTGGA GGCAGAGAAG GAGGCTGTGG CAGAGGCAGT GCGGGCCCTG 
CTGGCAAACC AAGACAGTGA CCAAGTGCAG AAGGACCCCC AATATCAGGG CCTGCGGGCC 
CGAGGCCGGG AGATCCGGAA GCAGCTCACA CCCTTGTACC CCCAAGAGAC CCAGCTAGAG 
GAGCAGCTGT ACCAGCAGGC ACTGAGGCTG CCCAACCAGA CCCACCCAGA CACGCCTGTG 
GGGGACGAGA GCCAGGCTAG AGTGGTCCGT GTGGTGGGTG AAAAGCCGGC TTTCTCCTTC 
CAACCCCGAG GCCACCTGGA AATTGGCGAG AAACTGGACA TCATCCGGCA GAAGCGCCTG 
TCTCACGTGT CTGGCCACCG GTCCTATTAC CTGCGTGGGG CAGGAGCCCT CTTACAGCAC 
GGCCTGGTCA ACTTCACCCT CAGCAAGCTT GTCAGCAGGG GTTGGGGGCA CCTGAGGAAC 
ACAGTGTCCA CTGAGGCCAG GAGTCTGTGC CATCAAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540438.1
CDS	49..870
Translation	MAASMARLWWPFLARQGLRSRGRCVCSQNPRRSFATEKRVRNLLYEHAREGYSELPYLDMESVCACPEKAARSLELRKGELRPADLPAIISTWQELRQLREQIRSLEAEKEAVAEAVRALLANQDSDQVQKDPQYQGLRARGREIRKQLTPLYPQETQLEEQLYQQALRLPNQTHPDTPVGDESQARVVRVVGEKPAFSFQPRGHLEIGEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTLSKLVSRGWGHLRNTVSTEARSLCHQS

Target ORF information:

RefSeq Version	XM_006540375.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540375.3

ORF Insert Sequence:

ATGGCTGCGT CCATGGCGCG GCTTTGGTGG CCTTTCCTGG CTCGCCAGGG GCTCCGGTCC 
CGAGGACGCT GCGTGTGCAG CCAGAACCCA AGGAGGAGTT TCGCTACGGA GAAACGAGTC 
CGGAACCTCC TGTACGAACA CGCACGAGAG GGCTACAGCG AGCTTCCCTA CTTGGACATG 
GAGTCGGTGT GCGCATGCCC AGAAAAAGCC GCGCGCTCCC TGGAGCTCCG CAAGGGGGAG 
CTGCGGCCGG CCGACCTGCC TGCGATTATC TCCACGTGGC AGGAGTTGAG GCAGCTTCGA 
GAGCAGATCC GGAGCCTGGA GGCAGAGAAG GAGGCTGTGG CAGAGGCAGT GCGGGCCCTG 
CTGGCAAACC AAGACAGTGA CCAAGTGCAG AAGGACCCCC AATATCAGGG CCTGCGGGCC 
CGAGGCCGGG AGATCCGGAA GCAGCTCACA CCCTTGTACC CCCAAGAGAC CCAGCTAGAG 
GAGCAGCTGT ACCAGCAGGC ACTGAGGCTG CCCAACCAGA CCCACCCAGA CACGCCTGTG 
GGGGACGAGA GCCAGGCTAG AGTGGTCCGT GTGGTGGGTG AAAAGCCGGC TTTCTCCTTC 
CAACCCCGAG GCCACCTGGA AATTGGCGAG AAACTGGACA TCATCCGGCA GAAGCGCCTG 
TCTCACGTGT CTGGCCACCG GTCCTATTAC CTGCGTGGGG CAGGAGCCCT CTTACAGCAC 
GGCCTGGTCA ACTTCACCCT CAGCAAGCTT GTCAGCAGGG GTTGGGGGCA CCTGAGGAAC 
ACAGTGTCCA CTGAGGCCAG GAGTCTGTGC CATCAAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sars2 ( XM_011250711.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011249013.1 Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu70810
Clone ID Related Accession (Same CDS sequence)	XM_011250711.2
Accession Version	XM_011250711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CCATGGCGCG GCTTTGGTGG CCTTTCCTGG CTCGCCAGGG GCTCCGGTCC 
CGAGGACGCT GCGTGTGCAG CCAGAACCCA AGGAGGAGTT TCGCTACGGA GAAACGAGTC 
CGGAACCTCC TGTACGAACA CGCACGAGAG GGCTACAGCG AGCTTCCCTA CTTGGACATG 
GAGTCGGTGT GCGCATGCCC AGAAAAAGCC GCGCGCTCCC TGGAGCTCCG CAAGGGGGAG 
CTGCGGCCGG CCGACCTGCC TGCGATTATC TCCACGTGGC AGGAGTTGAG GCAGCTTCGA 
GAGCAGATCC GGAGCCTGGA GGCAGAGAAG GAGGCTGTGG CAGAGGCAGT GCGGGCCCTG 
CTGGCAAACC AAGACAGTGA CCAAGTGCAG AAGGACCCCC AATATCAGGG CCTGCGGGCC 
CGAGGCCGGG AGATCCGGAA GCAGCTCACA CCCTTGTACC CCCAAGAGAC CCAGCTAGAG 
GAGCAGCTGT ACCAGCAGGC ACTGAGGCTG CCCAACCAGA CCCACCCAGA CACGCCTGTG 
GGGGACGAGA GCCAGGCTAG AGTGGTCCGT GTGGTGGGTG AAAAGCCGGC TTTCTCCTTC 
CAACCCCGAG GCCACCTGGA AATTGGCGAG AAACTGGACA TCATCCGGCA GAAGCGCCTG 
TCTCACGTGT CTGGCCACCG GTCCTATTAC CTGCGTGGGG CAGGAGCCCT CTTACAGCAC 
GGCCTGGTCA ACTTCACCCT CAGCAAGCTT GTCAGCAGGG GCTTCACCCC CATGACGGTC 
CCAGACCTTC TGAGAGGAGC TGTGTTTGCT GTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011249013.1
CDS	49..867
Translation	MAASMARLWWPFLARQGLRSRGRCVCSQNPRRSFATEKRVRNLLYEHAREGYSELPYLDMESVCACPEKAARSLELRKGELRPADLPAIISTWQELRQLREQIRSLEAEKEAVAEAVRALLANQDSDQVQKDPQYQGLRARGREIRKQLTPLYPQETQLEEQLYQQALRLPNQTHPDTPVGDESQARVVRVVGEKPAFSFQPRGHLEIGEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTLSKLVSRGFTPMTVPDLLRGAVFAVE

Target ORF information:

RefSeq Version	XM_011250711.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250711.2

ORF Insert Sequence:

ATGGCTGCGT CCATGGCGCG GCTTTGGTGG CCTTTCCTGG CTCGCCAGGG GCTCCGGTCC 
CGAGGACGCT GCGTGTGCAG CCAGAACCCA AGGAGGAGTT TCGCTACGGA GAAACGAGTC 
CGGAACCTCC TGTACGAACA CGCACGAGAG GGCTACAGCG AGCTTCCCTA CTTGGACATG 
GAGTCGGTGT GCGCATGCCC AGAAAAAGCC GCGCGCTCCC TGGAGCTCCG CAAGGGGGAG 
CTGCGGCCGG CCGACCTGCC TGCGATTATC TCCACGTGGC AGGAGTTGAG GCAGCTTCGA 
GAGCAGATCC GGAGCCTGGA GGCAGAGAAG GAGGCTGTGG CAGAGGCAGT GCGGGCCCTG 
CTGGCAAACC AAGACAGTGA CCAAGTGCAG AAGGACCCCC AATATCAGGG CCTGCGGGCC 
CGAGGCCGGG AGATCCGGAA GCAGCTCACA CCCTTGTACC CCCAAGAGAC CCAGCTAGAG 
GAGCAGCTGT ACCAGCAGGC ACTGAGGCTG CCCAACCAGA CCCACCCAGA CACGCCTGTG 
GGGGACGAGA GCCAGGCTAG AGTGGTCCGT GTGGTGGGTG AAAAGCCGGC TTTCTCCTTC 
CAACCCCGAG GCCACCTGGA AATTGGCGAG AAACTGGACA TCATCCGGCA GAAGCGCCTG 
TCTCACGTGT CTGGCCACCG GTCCTATTAC CTGCGTGGGG CAGGAGCCCT CTTACAGCAC 
GGCCTGGTCA ACTTCACCCT CAGCAAGCTT GTCAGCAGGG GCTTCACCCC CATGACGGTC 
CCAGACCTTC TGAGAGGAGC TGTGTTTGCT GTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sars2 ( XM_006540375.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540438.1 Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38509
Clone ID Related Accession (Same CDS sequence)	XM_006540375.3 , XM_006540375.4
Accession Version	XM_006540375.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540375.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CCATGGCGCG GCTTTGGTGG CCTTTCCTGG CTCGCCAGGG GCTCCGGTCC 
CGAGGACGCT GCGTGTGCAG CCAGAACCCA AGGAGGAGTT TCGCTACGGA GAAACGAGTC 
CGGAACCTCC TGTACGAACA CGCACGAGAG GGCTACAGCG AGCTTCCCTA CTTGGACATG 
GAGTCGGTGT GCGCATGCCC AGAAAAAGCC GCGCGCTCCC TGGAGCTCCG CAAGGGGGAG 
CTGCGGCCGG CCGACCTGCC TGCGATTATC TCCACGTGGC AGGAGTTGAG GCAGCTTCGA 
GAGCAGATCC GGAGCCTGGA GGCAGAGAAG GAGGCTGTGG CAGAGGCAGT GCGGGCCCTG 
CTGGCAAACC AAGACAGTGA CCAAGTGCAG AAGGACCCCC AATATCAGGG CCTGCGGGCC 
CGAGGCCGGG AGATCCGGAA GCAGCTCACA CCCTTGTACC CCCAAGAGAC CCAGCTAGAG 
GAGCAGCTGT ACCAGCAGGC ACTGAGGCTG CCCAACCAGA CCCACCCAGA CACGCCTGTG 
GGGGACGAGA GCCAGGCTAG AGTGGTCCGT GTGGTGGGTG AAAAGCCGGC TTTCTCCTTC 
CAACCCCGAG GCCACCTGGA AATTGGCGAG AAACTGGACA TCATCCGGCA GAAGCGCCTG 
TCTCACGTGT CTGGCCACCG GTCCTATTAC CTGCGTGGGG CAGGAGCCCT CTTACAGCAC 
GGCCTGGTCA ACTTCACCCT CAGCAAGCTT GTCAGCAGGG GTTGGGGGCA CCTGAGGAAC 
ACAGTGTCCA CTGAGGCCAG GAGTCTGTGC CATCAAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540438.1
CDS	60..881
Translation	MAASMARLWWPFLARQGLRSRGRCVCSQNPRRSFATEKRVRNLLYEHAREGYSELPYLDMESVCACPEKAARSLELRKGELRPADLPAIISTWQELRQLREQIRSLEAEKEAVAEAVRALLANQDSDQVQKDPQYQGLRARGREIRKQLTPLYPQETQLEEQLYQQALRLPNQTHPDTPVGDESQARVVRVVGEKPAFSFQPRGHLEIGEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTLSKLVSRGWGHLRNTVSTEARSLCHQS

Target ORF information:

RefSeq Version	XM_006540375.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540375.4

ORF Insert Sequence:

ATGGCTGCGT CCATGGCGCG GCTTTGGTGG CCTTTCCTGG CTCGCCAGGG GCTCCGGTCC 
CGAGGACGCT GCGTGTGCAG CCAGAACCCA AGGAGGAGTT TCGCTACGGA GAAACGAGTC 
CGGAACCTCC TGTACGAACA CGCACGAGAG GGCTACAGCG AGCTTCCCTA CTTGGACATG 
GAGTCGGTGT GCGCATGCCC AGAAAAAGCC GCGCGCTCCC TGGAGCTCCG CAAGGGGGAG 
CTGCGGCCGG CCGACCTGCC TGCGATTATC TCCACGTGGC AGGAGTTGAG GCAGCTTCGA 
GAGCAGATCC GGAGCCTGGA GGCAGAGAAG GAGGCTGTGG CAGAGGCAGT GCGGGCCCTG 
CTGGCAAACC AAGACAGTGA CCAAGTGCAG AAGGACCCCC AATATCAGGG CCTGCGGGCC 
CGAGGCCGGG AGATCCGGAA GCAGCTCACA CCCTTGTACC CCCAAGAGAC CCAGCTAGAG 
GAGCAGCTGT ACCAGCAGGC ACTGAGGCTG CCCAACCAGA CCCACCCAGA CACGCCTGTG 
GGGGACGAGA GCCAGGCTAG AGTGGTCCGT GTGGTGGGTG AAAAGCCGGC TTTCTCCTTC 
CAACCCCGAG GCCACCTGGA AATTGGCGAG AAACTGGACA TCATCCGGCA GAAGCGCCTG 
TCTCACGTGT CTGGCCACCG GTCCTATTAC CTGCGTGGGG CAGGAGCCCT CTTACAGCAC 
GGCCTGGTCA ACTTCACCCT CAGCAAGCTT GTCAGCAGGG GTTGGGGGCA CCTGAGGAAC 
ACAGTGTCCA CTGAGGCCAG GAGTCTGTGC CATCAAAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sars2 ( NM_023637.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076126.2 Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), mRNA.

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CloneID	OMu15593
Clone ID Related Accession (Same CDS sequence)	NM_023637.3
Accession Version	NM_023637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	serine--tRNA ligase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX122368.1, AK010491.1 and BY649585.1. On Mar 18, 2011 this sequence version replaced NM_023637.2. ##Evidence-Data-START## Transcript exon combination :: AK140760.1, BC079664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGT CCATGGCGCG GCTTTGGTGG CCTTTCCTGG CTCGCCAGGG GCTCCGGTCC 
CGAGGACGCT GCGTGTGCAG CCAGAACCCA AGGAGGAGTT TCGCTACGGA GAAACGAGTC 
CGGAACCTCC TGTACGAACA CGCACGAGAG GGCTACAGCG AGCTTCCCTA CTTGGACATG 
GAGTCGGTGT GCGCATGCCC AGAAAAAGCC GCGCGCTCCC TGGAGCTCCG CAAGGGGGAG 
CTGCGGCCGG CCGACCTGCC TGCGATTATC TCCACGTGGC AGGAGTTGAG GCAGCTTCGA 
GAGCAGATCC GGAGCCTGGA GGCAGAGAAG GAGGCTGTGG CAGAGGCAGT GCGGGCCCTG 
CTGGCAAACC AAGACAGTGA CCAAGTGCAG AAGGACCCCC AATATCAGGG CCTGCGGGCC 
CGAGGCCGGG AGATCCGGAA GCAGCTCACA CCCTTGTACC CCCAAGAGAC CCAGCTAGAG 
GAGCAGCTGT ACCAGCAGGC ACTGAGGCTG CCCAACCAGA CCCACCCAGA CACGCCTGTG 
GGGGACGAGA GCCAGGCTAG AGTGGTCCGT GTGGTGGGTG AAAAGCCGGC TTTCTCCTTC 
CAACCCCGAG GCCACCTGGA AATTGGCGAG AAACTGGACA TCATCCGGCA GAAGCGCCTG 
TCTCACGTGT CTGGCCACCG GTCCTATTAC CTGCGTGGGG CAGGAGCCCT CTTACAGCAC 
GGCCTGGTCA ACTTCACCCT CAGCAAGCTT GTCAGCAGGG GCTTCACCCC CATGACGGTC 
CCAGACCTTC TGAGAGGAGC TGTGTTTGAA GGCTGTGGAA TGACACCAAA TGCCAACCCA 
TCCCAGATTT ACAATATTGA CCCCTCTCGC TTCGAAGACC TTAACCTGGC CGGGACGGCA 
GAGGTGGGGC TGGCAGGATA CTTCATGGAC CACTCTGTAG CTTTCAGGGA CCTTCCAGTC 
AGGATGGTCT GTGCCAGCAC CTGCTACCGG GCAGAGACAG ACACTGGGAA GGAGCCTTGG 
GGACTGTACC GAGTCCATCA CTTCACTAAG GTGGAGATGT TTGGGGTGAC AGGCCCTGGG 
CTGGAGCAGA GCTCGCAGCT GTTGGATGAG TTCCTGTCTC TGCAGGTGGA GATCTTGACG 
GAGCTGGGCT TGCACTTCCG GGTACTGGAC ATGCCCACCC AGGAACTGGG CCTTCCTGCT 
TATCGCAAGT TCGACATTGA GGCCTGGATG CCTGGCCGAG GCCGCTATGG AGAGGTCACC 
AGTGCCTCCA ACTGCACAGA CTTCCAGAGC CGCCGCCTGT ACATCATGTT TGAGACAGAG 
ACCGGGGAGC TGCAGTTCGC ACACACGGTG AATGCCACCG CTTGTGCTGT TCCCCGAGTC 
CTCATTGCCC TCCTGGAAAG CAATCAGCAA AAGGATGGTT CAGTCCTCGT GCCCGCCGCT 
CTCCAGCCCT ACCTAGGCAC GGACCGGATC ACAGCCCCTA CCCATGTGCC TCTCCAGTAC 
ATCGGCCCCA ATCAGCCTCA GAAGCCCAGG CTCCCCGGCC AGTCTGCCAC CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076126.2
CDS	49..1605
Translation	MAASMARLWWPFLARQGLRSRGRCVCSQNPRRSFATEKRVRNLLYEHAREGYSELPYLDMESVCACPEKAARSLELRKGELRPADLPAIISTWQELRQLREQIRSLEAEKEAVAEAVRALLANQDSDQVQKDPQYQGLRARGREIRKQLTPLYPQETQLEEQLYQQALRLPNQTHPDTPVGDESQARVVRVVGEKPAFSFQPRGHLEIGEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTLSKLVSRGFTPMTVPDLLRGAVFEGCGMTPNANPSQIYNIDPSRFEDLNLAGTAEVGLAGYFMDHSVAFRDLPVRMVCASTCYRAETDTGKEPWGLYRVHHFTKVEMFGVTGPGLEQSSQLLDEFLSLQVEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRYGEVTSASNCTDFQSRRLYIMFETETGELQFAHTVNATACAVPRVLIALLESNQQKDGSVLVPAALQPYLGTDRITAPTHVPLQYIGPNQPQKPRLPGQSATR

Target ORF information:

RefSeq Version	NM_023637.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus seryl-aminoacyl-tRNA synthetase 2 (Sars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023637.3

ORF Insert Sequence:

ATGGCTGCGT CCATGGCGCG GCTTTGGTGG CCTTTCCTGG CTCGCCAGGG GCTCCGGTCC 
CGAGGACGCT GCGTGTGCAG CCAGAACCCA AGGAGGAGTT TCGCTACGGA GAAACGAGTC 
CGGAACCTCC TGTACGAACA CGCACGAGAG GGCTACAGCG AGCTTCCCTA CTTGGACATG 
GAGTCGGTGT GCGCATGCCC AGAAAAAGCC GCGCGCTCCC TGGAGCTCCG CAAGGGGGAG 
CTGCGGCCGG CCGACCTGCC TGCGATTATC TCCACGTGGC AGGAGTTGAG GCAGCTTCGA 
GAGCAGATCC GGAGCCTGGA GGCAGAGAAG GAGGCTGTGG CAGAGGCAGT GCGGGCCCTG 
CTGGCAAACC AAGACAGTGA CCAAGTGCAG AAGGACCCCC AATATCAGGG CCTGCGGGCC 
CGAGGCCGGG AGATCCGGAA GCAGCTCACA CCCTTGTACC CCCAAGAGAC CCAGCTAGAG 
GAGCAGCTGT ACCAGCAGGC ACTGAGGCTG CCCAACCAGA CCCACCCAGA CACGCCTGTG 
GGGGACGAGA GCCAGGCTAG AGTGGTCCGT GTGGTGGGTG AAAAGCCGGC TTTCTCCTTC 
CAACCCCGAG GCCACCTGGA AATTGGCGAG AAACTGGACA TCATCCGGCA GAAGCGCCTG 
TCTCACGTGT CTGGCCACCG GTCCTATTAC CTGCGTGGGG CAGGAGCCCT CTTACAGCAC 
GGCCTGGTCA ACTTCACCCT CAGCAAGCTT GTCAGCAGGG GCTTCACCCC CATGACGGTC 
CCAGACCTTC TGAGAGGAGC TGTGTTTGAA GGCTGTGGAA TGACACCAAA TGCCAACCCA 
TCCCAGATTT ACAATATTGA CCCCTCTCGC TTCGAAGACC TTAACCTGGC CGGGACGGCA 
GAGGTGGGGC TGGCAGGATA CTTCATGGAC CACTCTGTAG CTTTCAGGGA CCTTCCAGTC 
AGGATGGTCT GTGCCAGCAC CTGCTACCGG GCAGAGACAG ACACTGGGAA GGAGCCTTGG 
GGACTGTACC GAGTCCATCA CTTCACTAAG GTGGAGATGT TTGGGGTGAC AGGCCCTGGG 
CTGGAGCAGA GCTCGCAGCT GTTGGATGAG TTCCTGTCTC TGCAGGTGGA GATCTTGACG 
GAGCTGGGCT TGCACTTCCG GGTACTGGAC ATGCCCACCC AGGAACTGGG CCTTCCTGCT 
TATCGCAAGT TCGACATTGA GGCCTGGATG CCTGGCCGAG GCCGCTATGG AGAGGTCACC 
AGTGCCTCCA ACTGCACAGA CTTCCAGAGC CGCCGCCTGT ACATCATGTT TGAGACAGAG 
ACCGGGGAGC TGCAGTTCGC ACACACGGTG AATGCCACCG CTTGTGCTGT TCCCCGAGTC 
CTCATTGCCC TCCTGGAAAG CAATCAGCAA AAGGATGGTT CAGTCCTCGT GCCCGCCGCT 
CTCCAGCCCT ACCTAGGCAC GGACCGGATC ACAGCCCCTA CCCATGTGCC TCTCCAGTAC 
ATCGGCCCCA ATCAGCCTCA GAAGCCCAGG CTCCCCGGCC AGTCTGCCAC CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)
Gineste C,De Winter JM,Kohl C,Witt CC,Giannesini B,Brohm K,Le Fur Y,Gretz N,Vilmen C,Pecchi E,Jubeau M,Cozzone PJ,Stienen GJ,Granzier H,Labeit S,Ottenheijm CA,Bendahan D,Gondin J

The bidirectional promoter of two genes for the mitochondrial translational apparatus in mouse is regulated by an array of CCAAT boxes interacting with the transcription factor NF-Y.
Nucleic acids research35(2)664-77(2007)
Zanotto E,Shah ZH,Jacobs HT

Genomic organization, expression, and subcellular localization of mouse mitochondrial seryl-tRNA synthetase.
Biochemical and biophysical research communications317(3)774-8(2004 May)
Gibbons WJ Jr,Yan Q,Li R,Li X,Guan MX

Characterization and tRNA recognition of mammalian mitochondrial seryl-tRNA synthetase.
The Journal of biological chemistry275(26)19913-20(2000 Jun)
Yokogawa T,Shimada N,Takeuchi N,Benkowski L,Suzuki T,Omori A,Ueda T,Nishikawa K,Spremulli LL,Watanabe K

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Sars2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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