Rnf135 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf135
Entrez Gene ID	71956
Full Name	ring finger protein 135
Synonyms	0610037N03Rik,2410006N06Rik
General protein information	Preferred Names ring finger protein 135 Names E3 ubiquitin-protein ligase RNF135 RING-type E3 ubiquitin transferase RNF135 Riplet U 2-3-0
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 47.59 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_028019.4	NP_082295.1	E3 ubiquitin-protein ligase RNF135
NM_028019.3	NP_082295.1	E3 ubiquitin-protein ligase RNF135

Pathways from BioSystems

Homologies

	RNF135	XP_001112652.2
Canis lupus familiaris (dog)	RNF135	XP_005624884.1
Rattus norvegicus (Norway rat)	Rnf135	NP_001012010.1
Homo sapiens (human)	RNF135	NP_115698.3
Mus musculus (house mouse)	Rnf135	NP_082295.1
Bos taurus (cattle)	RNF135	NP_001092406.1
Pan troglodytes (chimpanzee)	RNF135	XP_001174802.1

Gene Ontology
Bibliography

Related articles in PubMed

Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity.
Shi Y, Yuan B, Zhu W, Zhang R, Li L, Hao X, Chen S, Hou F
Nature communications815138(2017 May)

A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses.
Oshiumi H, Miyashita M, Matsumoto M, Seya T
PLoS pathogens9(8)e1003533(2013)

The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection.
Oshiumi H, Miyashita M, Inoue N, Okabe M, Matsumoto M, Seya T
Cell host & microbe8(6)496-509(2010 Dec)

Evidence by expression analysis of candidate genes for congenital heart defects in the NF1 microdeletion interval.
Venturin M, Bentivegna A, Moroni R, Larizza L, Riva P
Annals of human genetics69(Pt 5)508-16(2005 Sep)

Complete physical map and gene content of the human NF1 tumor suppressor region in human and mouse.
Jenne DE, Tinschert S, Dorschner MO, Hameister H, Stephens K, Kehrer-Sawatzki H
Genes, chromosomes & cancer37(2)111-20(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Riplet-mediated K63-linked polyubiquitination released RIG-I RD autorepression, which allowed the access of positive factors to the RIG-I protein.
Title: A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses.

Riplet is essential for regulating RIG-I-mediated innate immune response against RNA virus infection in vivo.
Title: The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection.

The following Rnf135 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf135 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15831	NM_028019.4 Latest version!	Mus musculus ring finger protein 135 (Rnf135), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OMu15831	NM_028019.3	Mus musculus ring finger protein 135 (Rnf135), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf135 ( NM_028019.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082295.1 Mus musculus ring finger protein 135 (Rnf135), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15831
Clone ID Related Accession (Same CDS sequence)	NM_028019.4 , NM_028019.3
Accession Version	NM_028019.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-23
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF135
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591426.17. On Jul 4, 2018 this sequence version replaced NM_028019.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK010429.1, AK152096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG TTTGTTCTGG CAATGCAGTC CCCGTGTGGC TGAGCGAGGA CGACCTGAGC 
TGCATTATCT GCCAAGGGCT ACTAGACCAG CCCACCACGC TGCCCTGTGG CCACAGCTTC 
TGTCTCCGGT GCCTCCATGA CCTGTGGGTC TCGAAGCGTG GGGCCGTGGA TGGCTGCCCT 
TGGGCTTGCC CCATATGTCG GAAGGGTCCC TTGACAAAGC CCAAGCTGCA CAAGAACCCG 
CTGCTGCAAG ACCTGGTGGA CAAGTACCTC CAAGCGGCGC GGGAGGTCGA GGCTGGCTCG 
GAGCCTGAAC CCGCCCCCGC GCCGCGTTCT GCGCCCCAGG TGACAGTACA GAAAAGTACC 
ACAAACGTTA TCCAGGAGCT GACTGACATG GTGAGACAGC TTGTAGACGA TGTCAAGAGC 
CTTCAAACGC AAAGACCCAA CTTGGGATCT GGACAGGACA ATGCACAAGG CACCCCACCC 
ACGGATTCTT CCTCTGAGGG GGAACATTCC TTGGATTCTC CAAAGCTGGT GACCTTCAGT 
ATATCTCAGA AGAAAATTCA GGAGATTCTC CATAACCTGG AAGAAATTCA GGAGAAACTG 
CAAGGGAGTG TCCCAGGGAG AGCGCCCCCT AGAGAGCGAG TTCAGGAAAT GACATCTTCC 
TTGTGCCTGC TGCCTGACCA GAGGCGTCCT GCACCCAGGA AAGCTTCTCA CTTGTCCCTG 
TGGGCCATCA GTCCAACCTT TGACCTGAGG ACCCTCTCCT ATAACCTGGA GGTTTCCAAC 
AATAGCCGGA GAGTGACTGT GTCTCGTGGT GATCTGCACA CCTATCACTG GAGTCCCCAG 
AGATTTTCAA TTAGCCAGGT CTTTTGTTCT CAGGCCCTCT CTTCTGGACA GAAGTACTGG 
GAAGTGGACA CTAGGAACTG TAGCCACTGG GCCATCGGGG TAGCTTCTTG GGGCATGAAG 
CGGGACGGGA TGCTGGGAAG GACTATGGAC TCCTGGTGTA TAGAGTGGAG AGGGCCTGGC 
CAGTTCTCTG CTTGGGCCAA GATGAAGAAA ACAGACTTGC AGTCGGACCT CCCTGAGGTT 
GTGGGCGTGT GGCTGGACCT GGAGTCGGGG GAGCTTGCCT TCTACGCAGT GGCCGATCAC 
GAGAGGCTCC TGTATGAGTG TGAGGTCTCT AGCTCCTCTC CTCTGCATCC CGCCTTCTGG 
CTGTATGGCT TAAGTCCTGG AAACTACCTG GAAATAAAGC AGCTAAACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082295.1
CDS	49..1302
Translation	MAAVCSGNAVPVWLSEDDLSCIICQGLLDQPTTLPCGHSFCLRCLHDLWVSKRGAVDGCPWACPICRKGPLTKPKLHKNPLLQDLVDKYLQAAREVEAGSEPEPAPAPRSAPQVTVQKSTTNVIQELTDMVRQLVDDVKSLQTQRPNLGSGQDNAQGTPPTDSSSEGEHSLDSPKLVTFSISQKKIQEILHNLEEIQEKLQGSVPGRAPPRERVQEMTSSLCLLPDQRRPAPRKASHLSLWAISPTFDLRTLSYNLEVSNNSRRVTVSRGDLHTYHWSPQRFSISQVFCSQALSSGQKYWEVDTRNCSHWAIGVASWGMKRDGMLGRTMDSWCIEWRGPGQFSAWAKMKKTDLQSDLPEVVGVWLDLESGELAFYAVADHERLLYECEVSSSSPLHPAFWLYGLSPGNYLEIKQLNT

Target ORF information:

RefSeq Version	NM_028019.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 135 (Rnf135), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028019.4

ORF Insert Sequence:

ATGGCGGCCG TTTGTTCTGG CAATGCAGTC CCCGTGTGGC TGAGCGAGGA CGACCTGAGC 
TGCATTATCT GCCAAGGGCT ACTAGACCAG CCCACCACGC TGCCCTGTGG CCACAGCTTC 
TGTCTCCGGT GCCTCCATGA CCTGTGGGTC TCGAAGCGTG GGGCCGTGGA TGGCTGCCCT 
TGGGCTTGCC CCATATGTCG GAAGGGTCCC TTGACAAAGC CCAAGCTGCA CAAGAACCCG 
CTGCTGCAAG ACCTGGTGGA CAAGTACCTC CAAGCGGCGC GGGAGGTCGA GGCTGGCTCG 
GAGCCTGAAC CCGCCCCCGC GCCGCGTTCT GCGCCCCAGG TGACAGTACA GAAAAGTACC 
ACAAACGTTA TCCAGGAGCT GACTGACATG GTGAGACAGC TTGTAGACGA TGTCAAGAGC 
CTTCAAACGC AAAGACCCAA CTTGGGATCT GGACAGGACA ATGCACAAGG CACCCCACCC 
ACGGATTCTT CCTCTGAGGG GGAACATTCC TTGGATTCTC CAAAGCTGGT GACCTTCAGT 
ATATCTCAGA AGAAAATTCA GGAGATTCTC CATAACCTGG AAGAAATTCA GGAGAAACTG 
CAAGGGAGTG TCCCAGGGAG AGCGCCCCCT AGAGAGCGAG TTCAGGAAAT GACATCTTCC 
TTGTGCCTGC TGCCTGACCA GAGGCGTCCT GCACCCAGGA AAGCTTCTCA CTTGTCCCTG 
TGGGCCATCA GTCCAACCTT TGACCTGAGG ACCCTCTCCT ATAACCTGGA GGTTTCCAAC 
AATAGCCGGA GAGTGACTGT GTCTCGTGGT GATCTGCACA CCTATCACTG GAGTCCCCAG 
AGATTTTCAA TTAGCCAGGT CTTTTGTTCT CAGGCCCTCT CTTCTGGACA GAAGTACTGG 
GAAGTGGACA CTAGGAACTG TAGCCACTGG GCCATCGGGG TAGCTTCTTG GGGCATGAAG 
CGGGACGGGA TGCTGGGAAG GACTATGGAC TCCTGGTGTA TAGAGTGGAG AGGGCCTGGC 
CAGTTCTCTG CTTGGGCCAA GATGAAGAAA ACAGACTTGC AGTCGGACCT CCCTGAGGTT 
GTGGGCGTGT GGCTGGACCT GGAGTCGGGG GAGCTTGCCT TCTACGCAGT GGCCGATCAC 
GAGAGGCTCC TGTATGAGTG TGAGGTCTCT AGCTCCTCTC CTCTGCATCC CGCCTTCTGG 
CTGTATGGCT TAAGTCCTGG AAACTACCTG GAAATAAAGC AGCTAAACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf135 ( NM_028019.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082295.1 Mus musculus ring finger protein 135 (Rnf135), mRNA.

Price & Availability↑

CloneID	OMu15831
Clone ID Related Accession (Same CDS sequence)	NM_028019.4 , NM_028019.3
Accession Version	NM_028019.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-28
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF135
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL591426.17. On Apr 30, 2007 this sequence version replaced NM_028019.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK010429.1, SRR1660813.242404.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCG TTTGTTCTGG CAATGCAGTC CCCGTGTGGC TGAGCGAGGA CGACCTGAGC 
TGCATTATCT GCCAAGGGCT ACTAGACCAG CCCACCACGC TGCCCTGTGG CCACAGCTTC 
TGTCTCCGGT GCCTCCATGA CCTGTGGGTC TCGAAGCGTG GGGCCGTGGA TGGCTGCCCT 
TGGGCTTGCC CCATATGTCG GAAGGGTCCC TTGACAAAGC CCAAGCTGCA CAAGAACCCG 
CTGCTGCAAG ACCTGGTGGA CAAGTACCTC CAAGCGGCGC GGGAGGTCGA GGCTGGCTCG 
GAGCCTGAAC CCGCCCCCGC GCCGCGTTCT GCGCCCCAGG TGACAGTACA GAAAAGTACC 
ACAAACGTTA TCCAGGAGCT GACTGACATG GTGAGACAGC TTGTAGACGA TGTCAAGAGC 
CTTCAAACGC AAAGACCCAA CTTGGGATCT GGACAGGACA ATGCACAAGG CACCCCACCC 
ACGGATTCTT CCTCTGAGGG GGAACATTCC TTGGATTCTC CAAAGCTGGT GACCTTCAGT 
ATATCTCAGA AGAAAATTCA GGAGATTCTC CATAACCTGG AAGAAATTCA GGAGAAACTG 
CAAGGGAGTG TCCCAGGGAG AGCGCCCCCT AGAGAGCGAG TTCAGGAAAT GACATCTTCC 
TTGTGCCTGC TGCCTGACCA GAGGCGTCCT GCACCCAGGA AAGCTTCTCA CTTGTCCCTG 
TGGGCCATCA GTCCAACCTT TGACCTGAGG ACCCTCTCCT ATAACCTGGA GGTTTCCAAC 
AATAGCCGGA GAGTGACTGT GTCTCGTGGT GATCTGCACA CCTATCACTG GAGTCCCCAG 
AGATTTTCAA TTAGCCAGGT CTTTTGTTCT CAGGCCCTCT CTTCTGGACA GAAGTACTGG 
GAAGTGGACA CTAGGAACTG TAGCCACTGG GCCATCGGGG TAGCTTCTTG GGGCATGAAG 
CGGGACGGGA TGCTGGGAAG GACTATGGAC TCCTGGTGTA TAGAGTGGAG AGGGCCTGGC 
CAGTTCTCTG CTTGGGCCAA GATGAAGAAA ACAGACTTGC AGTCGGACCT CCCTGAGGTT 
GTGGGCGTGT GGCTGGACCT GGAGTCGGGG GAGCTTGCCT TCTACGCAGT GGCCGATCAC 
GAGAGGCTCC TGTATGAGTG TGAGGTCTCT AGCTCCTCTC CTCTGCATCC CGCCTTCTGG 
CTGTATGGCT TAAGTCCTGG AAACTACCTG GAAATAAAGC AGCTAAACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082295.1
CDS	46..1299
Translation	MAAVCSGNAVPVWLSEDDLSCIICQGLLDQPTTLPCGHSFCLRCLHDLWVSKRGAVDGCPWACPICRKGPLTKPKLHKNPLLQDLVDKYLQAAREVEAGSEPEPAPAPRSAPQVTVQKSTTNVIQELTDMVRQLVDDVKSLQTQRPNLGSGQDNAQGTPPTDSSSEGEHSLDSPKLVTFSISQKKIQEILHNLEEIQEKLQGSVPGRAPPRERVQEMTSSLCLLPDQRRPAPRKASHLSLWAISPTFDLRTLSYNLEVSNNSRRVTVSRGDLHTYHWSPQRFSISQVFCSQALSSGQKYWEVDTRNCSHWAIGVASWGMKRDGMLGRTMDSWCIEWRGPGQFSAWAKMKKTDLQSDLPEVVGVWLDLESGELAFYAVADHERLLYECEVSSSSPLHPAFWLYGLSPGNYLEIKQLNT

Target ORF information:

RefSeq Version	NM_028019.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 135 (Rnf135), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028019.3

ORF Insert Sequence:

ATGGCGGCCG TTTGTTCTGG CAATGCAGTC CCCGTGTGGC TGAGCGAGGA CGACCTGAGC 
TGCATTATCT GCCAAGGGCT ACTAGACCAG CCCACCACGC TGCCCTGTGG CCACAGCTTC 
TGTCTCCGGT GCCTCCATGA CCTGTGGGTC TCGAAGCGTG GGGCCGTGGA TGGCTGCCCT 
TGGGCTTGCC CCATATGTCG GAAGGGTCCC TTGACAAAGC CCAAGCTGCA CAAGAACCCG 
CTGCTGCAAG ACCTGGTGGA CAAGTACCTC CAAGCGGCGC GGGAGGTCGA GGCTGGCTCG 
GAGCCTGAAC CCGCCCCCGC GCCGCGTTCT GCGCCCCAGG TGACAGTACA GAAAAGTACC 
ACAAACGTTA TCCAGGAGCT GACTGACATG GTGAGACAGC TTGTAGACGA TGTCAAGAGC 
CTTCAAACGC AAAGACCCAA CTTGGGATCT GGACAGGACA ATGCACAAGG CACCCCACCC 
ACGGATTCTT CCTCTGAGGG GGAACATTCC TTGGATTCTC CAAAGCTGGT GACCTTCAGT 
ATATCTCAGA AGAAAATTCA GGAGATTCTC CATAACCTGG AAGAAATTCA GGAGAAACTG 
CAAGGGAGTG TCCCAGGGAG AGCGCCCCCT AGAGAGCGAG TTCAGGAAAT GACATCTTCC 
TTGTGCCTGC TGCCTGACCA GAGGCGTCCT GCACCCAGGA AAGCTTCTCA CTTGTCCCTG 
TGGGCCATCA GTCCAACCTT TGACCTGAGG ACCCTCTCCT ATAACCTGGA GGTTTCCAAC 
AATAGCCGGA GAGTGACTGT GTCTCGTGGT GATCTGCACA CCTATCACTG GAGTCCCCAG 
AGATTTTCAA TTAGCCAGGT CTTTTGTTCT CAGGCCCTCT CTTCTGGACA GAAGTACTGG 
GAAGTGGACA CTAGGAACTG TAGCCACTGG GCCATCGGGG TAGCTTCTTG GGGCATGAAG 
CGGGACGGGA TGCTGGGAAG GACTATGGAC TCCTGGTGTA TAGAGTGGAG AGGGCCTGGC 
CAGTTCTCTG CTTGGGCCAA GATGAAGAAA ACAGACTTGC AGTCGGACCT CCCTGAGGTT 
GTGGGCGTGT GGCTGGACCT GGAGTCGGGG GAGCTTGCCT TCTACGCAGT GGCCGATCAC 
GAGAGGCTCC TGTATGAGTG TGAGGTCTCT AGCTCCTCTC CTCTGCATCC CGCCTTCTGG 
CTGTATGGCT TAAGTCCTGG AAACTACCTG GAAATAAAGC AGCTAAACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity.
Nature communications815138(2017 May)
Shi Y,Yuan B,Zhu W,Zhang R,Li L,Hao X,Chen S,Hou F

A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses.
PLoS pathogens9(8)e1003533(2013)
Oshiumi H,Miyashita M,Matsumoto M,Seya T

The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection.
Cell host & microbe8(6)496-509(2010 Dec)
Oshiumi H,Miyashita M,Inoue N,Okabe M,Matsumoto M,Seya T

Evidence by expression analysis of candidate genes for congenital heart defects in the NF1 microdeletion interval.
Annals of human genetics69(Pt 5)508-16(2005 Sep)
Venturin M,Bentivegna A,Moroni R,Larizza L,Riva P

Complete physical map and gene content of the human NF1 tumor suppressor region in human and mouse.
Genes, chromosomes & cancer37(2)111-20(2003 Jun)
Jenne DE,Tinschert S,Dorschner MO,Hameister H,Stephens K,Kehrer-Sawatzki H

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Rnf135 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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