Efhc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Efhc1
Entrez Gene ID	71877
Full Name	EF-hand domain (C-terminal) containing 1
Synonyms	1700029F22Rik,mRib72-1,myoclonin1
General protein information	Preferred Names EF-hand domain (C-terminal) containing 1 Names EF-hand domain-containing protein 1 myoclonin-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 A4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006495576.3	XP_006495639.1	EF-hand domain-containing protein 1 isoform X1
NM_027974.1	NP_082250.1	EF-hand domain-containing protein 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	EFHC1	XP_420047.2
Rattus norvegicus (Norway rat)	Efhc1	NP_001116419.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003805	XP_310360.7
Homo sapiens (human)	EFHC1	NP_060570.2
Pan troglodytes (chimpanzee)	EFHC1	XP_001152433.1
	EFHC1	XP_001107470.1
Canis lupus familiaris (dog)	EFHC1	XP_005627529.1
Mus musculus (house mouse)	Efhc1	NP_082250.1
Danio rerio (zebrafish)	efhc1	NP_957261.1
Bos taurus (cattle)	EFHC1	NP_001179173.1
Xenopus tropicalis (tropical clawed frog)	efhc1	NP_001008106.1

Gene Ontology
Bibliography

Related articles in PubMed

EFHC1 variants in juvenile myoclonic epilepsy: reanalysis according to NHGRI and ACMG guidelines for assigning disease causality.
Bailey JN, Patterson C, de Nijs L, Dur?n RM, Nguyen VH, Tanaka M, Medina MT, Jara-Prado A, Mart?nez-Ju?rez IE, Ochoa A, Molina Y, Suzuki T, Alonso ME, Wight JE, Lin YC, Guilhoto L, Targas Yacubian EM, Machado-Salas J, Daga A, Yamakawa K, Grisar TM, Lakaye B, Delgado-Escueta AV
Genetics in medicine : official journal of the American College of Medical Genetics19(2)144-156(2017 Feb)

Re-evaluation of myoclonin1 immunosignals in neuron, mitotic spindle, and midbody--nonspecific?
Yamakawa K, Suzuki T
Epilepsy & behavior : E&B28 Suppl 1S61-2(2013 Jul)

Mutations of EFHC1, linked to juvenile myoclonic epilepsy, disrupt radial and tangential migrations during brain development.
de Nijs L, Wolkoff N, Coumans B, Delgado-Escueta AV, Grisar T, Lakaye B
Human molecular genetics21(23)5106-17(2012 Dec)

Distribution of EFHC1 or Myoclonin 1 in mouse neural structures.
L?on C, de Nijs L, Chanas G, Delgado-Escueta AV, Grisar T, Lakaye B
Epilepsy research88(2-3)196-207(2010 Feb)

Tissue expression patterns identify mouse cilia genes.
McClintock TS, Glasser CE, Bose SC, Bergman DA
Physiological genomics32(2)198-206(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We reviewed whether variants damage EFHC1 functions, whether efhc1(-/-) KO mice recapitulate CTC convulsions and "microdysgenesis" neuropathology, and whether supernumerary synaptic and dendritic phenotypes can be rescued in the fly model when EFHC1 is overexpressed
Title: EFHC1 variants in juvenile myoclonic epilepsy: reanalysis according to NHGRI and ACMG guidelines for assigning disease causality.

Efhc1 (-/-) mouse did not show any abnormalities such as disruption of mitotic spindle structure, impaired M-phase progression, and an increase of apoptosis. Further investigations are required to clarify these discrepancies.
Title: Re-evaluation of myoclonin1 immunosignals in neuron, mitotic spindle, and midbody--nonspecific?

The juvenile myoclonic epilepsy-related protein EFHC1 interacts with the redox-sensitive TRPM2 channel linked to cell death.
Title: The juvenile myoclonic epilepsy-related protein EFHC1 interacts with the redox-sensitive TRPM2 channel linked to cell death.

In the adult, low mRNA expression was detected in several brain structures such as cortex, striatum, hippocampus and cerebellum. At E16, EFHC1 mRNA was shown to be expressed in all layers of cortex and not only in cells lining ventricles.
Title: Distribution of EFHC1 or Myoclonin 1 in mouse neural structures.

Data report the expression profile and distribution of Efhc1 messenger RNA during mouse and rat brain development, and suggest that Efhc1 expression is more important during initial phases of brain development.
Title: Expression profile and distribution of Efhc1 gene transcript during rodent brain development.

The following Efhc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Efhc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04880	NM_027974.1 Latest version!	Mus musculus EF-hand domain (C-terminal) containing 1 (Efhc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
OMu27179	XM_006495576.3 Latest version!	Mus musculus EF-hand domain (C-terminal) containing 1 (Efhc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Efhc1 ( NM_027974.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082250.1 Mus musculus EF-hand domain (C-terminal) containing 1 (Efhc1), mRNA.

Price & Availability↑

CloneID	OMu04880
Clone ID Related Accession (Same CDS sequence)	NM_027974.1
Accession Version	NM_027974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	EF-hand domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY037196.1, EU520262.1, AK006489.1 and AC156980.9. On or before Jul 23, 2009 this sequence version replaced XM_907335.2, XM_129694.8. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK006489.1, EU520262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCA ACCCGGTGCA CGGCCTGCCC TTTCTCCCGG GCTCGTCCTT TACAGACTCC 
ACGAAAACAG CATTCCACCG AAGCCAGACA CTCAACTACA GGAACGGCTA TGCAGTTGTC 
CGACGTCCCA CCATGGGGAT AGGTGGAGAC CGGCTCCACT ACAACCAGCT GTCTCAGGCC 
GAGCTGGATG AACTGGCCAA TAAGGCACCC ATCTTAACTT ACGGGCCACT CAAGCAAGCC 
CCACTTGCAG AGTTTGTTCC TGCGCATGTG GCTTTTGACA AAAAGGTGCT GAAATTTAGT 
GCCTACTTCC AAGAAGATGT TCCCATATCA ATGGAGGAGC ATTACCGAAT CCGCCACGTG 
AACATTTACT ACTACCTAGA AGATGACAGC ATGTCTGTCA TCGAGCCCGT AGTGGAGAAC 
TCTGGGATCC CACAAGGCAA GCTAATCAAA CGCCAGCGGT TCACCAAGAA TGATATGGGA 
GACCACTACC ACTGGAAGGA CCTCAACCGT GGAATCAACC TTACAGTGTA CGGCAAAACC 
TTCCGAATTG TTGACTGTGA CCGATTCACT CAGGACTTTT TGGAAAGCCA AGGCATTGAA 
TTAAATCCAT CAGAGAAGAT ACCCCTGGAT CCTTACACTC AACTCCGGAA AGAACCTGTG 
CGCAAATATG TCACCCCGTC AGACTTTGAC CAACTGAAAC AGTTTCTCAC CTTTGACAAA 
CAGGTCCTTC GATTCTATGC CATCTGGGAC GACACGGATA GCTTGTTCGG GGAATGTCGT 
CACTACATCA TTCATTACTA CCTCATGGAT GACACCGTGG AGATCAGAGA GGTCCACGAA 
CGGAACAACG GGAGAGACCC CTTCCCACTC CTCATGAACC GACAGCGTAT GCCCAAAGTT 
TTGGTGGAGA ATGCAAAGAA TTTCCCGAAG TGTGTGCTGG AAATCTCAGA CCAAGAGGTG 
CTGGAGTGGT ACACTGCCAA GGACTTCATT GTGGGGAAGC CGCTCACCAT CCTGGGGAGG 
ACCTTCTTCA TCTACGACTG TGACCCATTC ACACGACAGT TCTACAAAGA CAAGTTTGGA 
ATGCCTGACT TACCACCAGT TGATGTGACC AAGAAGGAGC CACCTCCAGT AAAACAGGAG 
TTGCCTCCTT ATAATGGCTA TGGACTAATT GAAGACTCTG CTCAGAATTG TTTTGCTCTC 
ATTCCTAAAG CTCCCAGAAA AGATGTCGTT AAAATGCTGA TGAATGACAA TAAGGTGCTC 
CGCTACCTGG CGGCCCTGGA ATCCCCTATC CCAGAAGACA AAGACCGTCG GTTTGTCTTC 
TCTTATTTTC TTGCCACGGA TATGATCAGT ATCTTTGAGC CACCTGTTCG AAATTCTGGG 
ATCATCGGGG GCAAATTCCT GGGCAGGACT AAAGTTGTTA AATCATTCTC TCCAGTTGAC 
AACCCCATCT ACTACTCACC AAGTGACTTC TTCATCGGTG CCGTGATTGA GGTGTTCGGC 
CATCGGTTCG TCATCCTCGA TACAGATGAG TATGTTTTAA AATACATGGA GAGCAACGCT 
TCCCAGTATT CACCAGAAGC CCTGGCCTCA ATTCAGAACC GGATCCAGAA GCCGGAGCTT 
CCCGCACCAG AACTGGAAAG CAAGCAAGCT ACAGGAGAAC CCATGGTGCA GGGTACAGAG 
GAATCCAAGG TGCAGGACTT GGATGCACTG ATAGACCAAA TCCATATGCA CCTGAAATAC 
AATTCATGCA AAGAAAACCT CCGTGAAACA TTTCAAATGT ATGACAAGGA TGAATCGGGA 
TATGTGGACA GAGAGACGTT CTTTAAAATC TGTGAAACTC TTAATGTCCC GGTCGATGAC 
TCGTTGATTA AGGAGTTGAT CAGGCTGTGC ACCCACGGAG AAGGCAGGAT TAACTACTAT 
AACTTTGTTC GAGCTTTCTC GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082250.1
CDS	109..2055
Translation	MGTNPVHGLPFLPGSSFTDSTKTAFHRSQTLNYRNGYAVVRRPTMGIGGDRLHYNQLSQAELDELANKAPILTYGPLKQAPLAEFVPAHVAFDKKVLKFSAYFQEDVPISMEEHYRIRHVNIYYYLEDDSMSVIEPVVENSGIPQGKLIKRQRFTKNDMGDHYHWKDLNRGINLTVYGKTFRIVDCDRFTQDFLESQGIELNPSEKIPLDPYTQLRKEPVRKYVTPSDFDQLKQFLTFDKQVLRFYAIWDDTDSLFGECRHYIIHYYLMDDTVEIREVHERNNGRDPFPLLMNRQRMPKVLVENAKNFPKCVLEISDQEVLEWYTAKDFIVGKPLTILGRTFFIYDCDPFTRQFYKDKFGMPDLPPVDVTKKEPPPVKQELPPYNGYGLIEDSAQNCFALIPKAPRKDVVKMLMNDNKVLRYLAALESPIPEDKDRRFVFSYFLATDMISIFEPPVRNSGIIGGKFLGRTKVVKSFSPVDNPIYYSPSDFFIGAVIEVFGHRFVILDTDEYVLKYMESNASQYSPEALASIQNRIQKPELPAPELESKQATGEPMVQGTEESKVQDLDALIDQIHMHLKYNSCKENLRETFQMYDKDESGYVDRETFFKICETLNVPVDDSLIKELIRLCTHGEGRINYYNFVRAFSN

Target ORF information:

RefSeq Version	NM_027974.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus EF-hand domain (C-terminal) containing 1 (Efhc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027974.1

ORF Insert Sequence:

ATGGGGACCA ACCCGGTGCA CGGCCTGCCC TTTCTCCCGG GCTCGTCCTT TACAGACTCC 
ACGAAAACAG CATTCCACCG AAGCCAGACA CTCAACTACA GGAACGGCTA TGCAGTTGTC 
CGACGTCCCA CCATGGGGAT AGGTGGAGAC CGGCTCCACT ACAACCAGCT GTCTCAGGCC 
GAGCTGGATG AACTGGCCAA TAAGGCACCC ATCTTAACTT ACGGGCCACT CAAGCAAGCC 
CCACTTGCAG AGTTTGTTCC TGCGCATGTG GCTTTTGACA AAAAGGTGCT GAAATTTAGT 
GCCTACTTCC AAGAAGATGT TCCCATATCA ATGGAGGAGC ATTACCGAAT CCGCCACGTG 
AACATTTACT ACTACCTAGA AGATGACAGC ATGTCTGTCA TCGAGCCCGT AGTGGAGAAC 
TCTGGGATCC CACAAGGCAA GCTAATCAAA CGCCAGCGGT TCACCAAGAA TGATATGGGA 
GACCACTACC ACTGGAAGGA CCTCAACCGT GGAATCAACC TTACAGTGTA CGGCAAAACC 
TTCCGAATTG TTGACTGTGA CCGATTCACT CAGGACTTTT TGGAAAGCCA AGGCATTGAA 
TTAAATCCAT CAGAGAAGAT ACCCCTGGAT CCTTACACTC AACTCCGGAA AGAACCTGTG 
CGCAAATATG TCACCCCGTC AGACTTTGAC CAACTGAAAC AGTTTCTCAC CTTTGACAAA 
CAGGTCCTTC GATTCTATGC CATCTGGGAC GACACGGATA GCTTGTTCGG GGAATGTCGT 
CACTACATCA TTCATTACTA CCTCATGGAT GACACCGTGG AGATCAGAGA GGTCCACGAA 
CGGAACAACG GGAGAGACCC CTTCCCACTC CTCATGAACC GACAGCGTAT GCCCAAAGTT 
TTGGTGGAGA ATGCAAAGAA TTTCCCGAAG TGTGTGCTGG AAATCTCAGA CCAAGAGGTG 
CTGGAGTGGT ACACTGCCAA GGACTTCATT GTGGGGAAGC CGCTCACCAT CCTGGGGAGG 
ACCTTCTTCA TCTACGACTG TGACCCATTC ACACGACAGT TCTACAAAGA CAAGTTTGGA 
ATGCCTGACT TACCACCAGT TGATGTGACC AAGAAGGAGC CACCTCCAGT AAAACAGGAG 
TTGCCTCCTT ATAATGGCTA TGGACTAATT GAAGACTCTG CTCAGAATTG TTTTGCTCTC 
ATTCCTAAAG CTCCCAGAAA AGATGTCGTT AAAATGCTGA TGAATGACAA TAAGGTGCTC 
CGCTACCTGG CGGCCCTGGA ATCCCCTATC CCAGAAGACA AAGACCGTCG GTTTGTCTTC 
TCTTATTTTC TTGCCACGGA TATGATCAGT ATCTTTGAGC CACCTGTTCG AAATTCTGGG 
ATCATCGGGG GCAAATTCCT GGGCAGGACT AAAGTTGTTA AATCATTCTC TCCAGTTGAC 
AACCCCATCT ACTACTCACC AAGTGACTTC TTCATCGGTG CCGTGATTGA GGTGTTCGGC 
CATCGGTTCG TCATCCTCGA TACAGATGAG TATGTTTTAA AATACATGGA GAGCAACGCT 
TCCCAGTATT CACCAGAAGC CCTGGCCTCA ATTCAGAACC GGATCCAGAA GCCGGAGCTT 
CCCGCACCAG AACTGGAAAG CAAGCAAGCT ACAGGAGAAC CCATGGTGCA GGGTACAGAG 
GAATCCAAGG TGCAGGACTT GGATGCACTG ATAGACCAAA TCCATATGCA CCTGAAATAC 
AATTCATGCA AAGAAAACCT CCGTGAAACA TTTCAAATGT ATGACAAGGA TGAATCGGGA 
TATGTGGACA GAGAGACGTT CTTTAAAATC TGTGAAACTC TTAATGTCCC GGTCGATGAC 
TCGTTGATTA AGGAGTTGAT CAGGCTGTGC ACCCACGGAG AAGGCAGGAT TAACTACTAT 
AACTTTGTTC GAGCTTTCTC GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Efhc1 ( XM_006495576.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495639.1 Mus musculus EF-hand domain (C-terminal) containing 1 (Efhc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27179
Clone ID Related Accession (Same CDS sequence)	XM_006495576.3
Accession Version	XM_006495576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	EF-hand domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495576.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACCA ACCCGGTGCA CGGCCTGCCC TTTCTCCCGG GCTCGTCCTT TACAGACTCC 
ACGAAAACAG CATTCCACCG AAGCCAGACA CTCAACTACA GGAACGGCTA TGCAGTTGTC 
CGACGTCCCA CCATGGGGAT AGGTGGAGAC CGGCTCCACT ACAACCAGCT GTCTCAGGCC 
GAGCTGGATG AACTGGCCAA TAAGGCACCC ATCTTAACTT ACGGGCCACT CAAGCAAGCC 
CCACTTGCAG AGTTTGTTCC TGCGCATGTG GCTTTTGACA AAAAGGTGCT GAAATTTAGT 
GCCTACTTCC AAGAAGATGT TCCCATATCA ATGGAGGAGC ATTACCGAAT CCGCCACGTG 
AACATTTACT ACTACCTAGA AGATGACAGC ATGTCTGTCA TCGAGCCCGT AGTGGAGAAC 
TCTGGGATCC CACAAGGCAA GCTAATCAAA CGCCAGCGGT TCACCAAGAA TGATATGGGA 
GACCACTACC ACTGGAAGGA CCTCAACCGT GGAATCAACC TTACAGTGTA CGGCAAAACC 
TTCCGAATTG TTGACTGTGA CCGATTCACT CAGGACTTTT TGGAAAGCCA AGGCATTGAA 
TTAAATCCAT CAGAGAAGAT ACCCCTGGAT CCTTACACTC AACTCCGGAA AGAACCTGTG 
CGCAAATATG TCACCCCGTC AGACTTTGAC CAACTGAAAC AGTTTCTCAC CTTTGACAAA 
CAGGTCCTTC GATTCTATGC CATCTGGGAC GACACGGATA GCTTGTTCGG GGAATGTCGT 
CACTACATCA TTCATTACTA CCTCATGGAT GACACCGTGG AGATCAGAGA GGTCCACGAA 
CGGAACAACG GGAGAGACCC CTTCCCACTC CTCATGAACC GACAGCGTAT GCCCAAAGTT 
TTGGTGGAGA ATGCAAAGAA TTTCCCGAAG TGTGTGCTGG AAATCTCAGA CCAAGAGGTG 
CTGGAGTGGT ACACTGCCAA GGACTTCATT GTGGGGAAGC CGCTCACCAT CCTGGGGAGG 
ACCTTCTTCA TCTACGACTG TGACCCATTC ACACGACAGT TCTACAAAGA CAAGTTTGGA 
ATGCCTGACT TACCACCAGT TGATGTGACC AAGAAGGAGC CACCTCCAGT AAAACAGGAG 
TTGCCTCCTT ATAATGGCTA TGGACTAATT GAAGACTCTG CTCAGAATTG TTTTGCTCTC 
ATTCCTAAAG CTCCCAGAAA AGATGTCGTT AAAATGCTGA TGAATGACAA TAAGGTGCTC 
CGCTACCTGG CGGCCCTGGA ATCCCCTATC CCAGAAGACA AAGACCGTCG GTTTGTCTTC 
TCTTATTTTC TTGCCACGGA TATGATCAGT ATCTTTGAGC CACCTGTTCG AAATTCTGGG 
ATCATCGGGG GCAAATTCCT GGGCAGGACT AAAGTTGTTA AATCATTCTC TCCAGTTGAC 
AACCCCATCT ACTACTCACC AAGTGACTTC TTCATCGGTG CCGTGATTGA GGGGTTGGCA 
TCATTTTGCT CCTGCTTGCA TGAGAGAAAC AAAGCTGAAT TGAGTGTGAT GCTCCTCTCT 
CCTCAGTGTT CGGCCATCGG TTCGTCATCC TCGATACAGA TGAGTATGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495639.1
CDS	185..1798
Translation	MGTNPVHGLPFLPGSSFTDSTKTAFHRSQTLNYRNGYAVVRRPTMGIGGDRLHYNQLSQAELDELANKAPILTYGPLKQAPLAEFVPAHVAFDKKVLKFSAYFQEDVPISMEEHYRIRHVNIYYYLEDDSMSVIEPVVENSGIPQGKLIKRQRFTKNDMGDHYHWKDLNRGINLTVYGKTFRIVDCDRFTQDFLESQGIELNPSEKIPLDPYTQLRKEPVRKYVTPSDFDQLKQFLTFDKQVLRFYAIWDDTDSLFGECRHYIIHYYLMDDTVEIREVHERNNGRDPFPLLMNRQRMPKVLVENAKNFPKCVLEISDQEVLEWYTAKDFIVGKPLTILGRTFFIYDCDPFTRQFYKDKFGMPDLPPVDVTKKEPPPVKQELPPYNGYGLIEDSAQNCFALIPKAPRKDVVKMLMNDNKVLRYLAALESPIPEDKDRRFVFSYFLATDMISIFEPPVRNSGIIGGKFLGRTKVVKSFSPVDNPIYYSPSDFFIGAVIEGLASFCSCLHERNKAELSVMLLSPQCSAIGSSSSIQMSMF

Target ORF information:

RefSeq Version	XM_006495576.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus EF-hand domain (C-terminal) containing 1 (Efhc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495576.3

ORF Insert Sequence:

ATGGGGACCA ACCCGGTGCA CGGCCTGCCC TTTCTCCCGG GCTCGTCCTT TACAGACTCC 
ACGAAAACAG CATTCCACCG AAGCCAGACA CTCAACTACA GGAACGGCTA TGCAGTTGTC 
CGACGTCCCA CCATGGGGAT AGGTGGAGAC CGGCTCCACT ACAACCAGCT GTCTCAGGCC 
GAGCTGGATG AACTGGCCAA TAAGGCACCC ATCTTAACTT ACGGGCCACT CAAGCAAGCC 
CCACTTGCAG AGTTTGTTCC TGCGCATGTG GCTTTTGACA AAAAGGTGCT GAAATTTAGT 
GCCTACTTCC AAGAAGATGT TCCCATATCA ATGGAGGAGC ATTACCGAAT CCGCCACGTG 
AACATTTACT ACTACCTAGA AGATGACAGC ATGTCTGTCA TCGAGCCCGT AGTGGAGAAC 
TCTGGGATCC CACAAGGCAA GCTAATCAAA CGCCAGCGGT TCACCAAGAA TGATATGGGA 
GACCACTACC ACTGGAAGGA CCTCAACCGT GGAATCAACC TTACAGTGTA CGGCAAAACC 
TTCCGAATTG TTGACTGTGA CCGATTCACT CAGGACTTTT TGGAAAGCCA AGGCATTGAA 
TTAAATCCAT CAGAGAAGAT ACCCCTGGAT CCTTACACTC AACTCCGGAA AGAACCTGTG 
CGCAAATATG TCACCCCGTC AGACTTTGAC CAACTGAAAC AGTTTCTCAC CTTTGACAAA 
CAGGTCCTTC GATTCTATGC CATCTGGGAC GACACGGATA GCTTGTTCGG GGAATGTCGT 
CACTACATCA TTCATTACTA CCTCATGGAT GACACCGTGG AGATCAGAGA GGTCCACGAA 
CGGAACAACG GGAGAGACCC CTTCCCACTC CTCATGAACC GACAGCGTAT GCCCAAAGTT 
TTGGTGGAGA ATGCAAAGAA TTTCCCGAAG TGTGTGCTGG AAATCTCAGA CCAAGAGGTG 
CTGGAGTGGT ACACTGCCAA GGACTTCATT GTGGGGAAGC CGCTCACCAT CCTGGGGAGG 
ACCTTCTTCA TCTACGACTG TGACCCATTC ACACGACAGT TCTACAAAGA CAAGTTTGGA 
ATGCCTGACT TACCACCAGT TGATGTGACC AAGAAGGAGC CACCTCCAGT AAAACAGGAG 
TTGCCTCCTT ATAATGGCTA TGGACTAATT GAAGACTCTG CTCAGAATTG TTTTGCTCTC 
ATTCCTAAAG CTCCCAGAAA AGATGTCGTT AAAATGCTGA TGAATGACAA TAAGGTGCTC 
CGCTACCTGG CGGCCCTGGA ATCCCCTATC CCAGAAGACA AAGACCGTCG GTTTGTCTTC 
TCTTATTTTC TTGCCACGGA TATGATCAGT ATCTTTGAGC CACCTGTTCG AAATTCTGGG 
ATCATCGGGG GCAAATTCCT GGGCAGGACT AAAGTTGTTA AATCATTCTC TCCAGTTGAC 
AACCCCATCT ACTACTCACC AAGTGACTTC TTCATCGGTG CCGTGATTGA GGGGTTGGCA 
TCATTTTGCT CCTGCTTGCA TGAGAGAAAC AAAGCTGAAT TGAGTGTGAT GCTCCTCTCT 
CCTCAGTGTT CGGCCATCGG TTCGTCATCC TCGATACAGA TGAGTATGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EFHC1 variants in juvenile myoclonic epilepsy: reanalysis according to NHGRI and ACMG guidelines for assigning disease causality.
Genetics in medicine : official journal of the American College of Medical Genetics19(2)144-156(2017 Feb)
Bailey JN,Patterson C,de Nijs L,Dur?n RM,Nguyen VH,Tanaka M,Medina MT,Jara-Prado A,Mart?nez-Ju?rez IE,Ochoa A,Molina Y,Suzuki T,Alonso ME,Wight JE,Lin YC,Guilhoto L,Targas Yacubian EM,Machado-Salas J,Daga A,Yamakawa K,Grisar TM,Lakaye B,Delgado-Escueta AV

Re-evaluation of myoclonin1 immunosignals in neuron, mitotic spindle, and midbody--nonspecific?
Epilepsy & behavior : E&B28 Suppl 1S61-2(2013 Jul)
Yamakawa K,Suzuki T

Mutations of EFHC1, linked to juvenile myoclonic epilepsy, disrupt radial and tangential migrations during brain development.
Human molecular genetics21(23)5106-17(2012 Dec)
de Nijs L,Wolkoff N,Coumans B,Delgado-Escueta AV,Grisar T,Lakaye B

Distribution of EFHC1 or Myoclonin 1 in mouse neural structures.
Epilepsy research88(2-3)196-207(2010 Feb)
L?on C,de Nijs L,Chanas G,Delgado-Escueta AV,Grisar T,Lakaye B

Tissue expression patterns identify mouse cilia genes.
Physiological genomics32(2)198-206(2008 Jan)
McClintock TS,Glasser CE,Bose SC,Bergman DA

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Efhc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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