Tysnd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tysnd1
Entrez Gene ID	71767
Full Name	trypsin domain containing 1
Synonyms	1300019N10Rik
General protein information	Preferred Names trypsin domain containing 1 Names peroxisomal leader peptide-processing protease trypsin domain-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B4
Summary	This gene encodes a protease that removes the N-terminal peroxisomal targeting signal (PTS2) from proteins produced in the cytosol, thereby facilitating their import into the peroxisome. The encoded protein is also capable of removing the C-terminal peroxisomal targeting signal (PTS1) from proteins in the peroxisomal matrix. The full-length protein undergoes self-cleavage to produce shorter, potentially inactive, peptides. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272091.1	NP_001259020.1	peroxisomal leader peptide-processing protease isoform c
XM_006514168.3	XP_006514231.1	peroxisomal leader peptide-processing protease isoform X1
NM_027912.1	NP_082188.1	peroxisomal leader peptide-processing protease isoform a
NM_001272090.1	NP_001259019.1	peroxisomal leader peptide-processing protease isoform b
NM_001272092.1	NP_001259021.1	peroxisomal leader peptide-processing protease isoform d

Pathways from BioSystems

Homologies

Homo sapiens (human)	TYSND1	NP_775826.2
Mus musculus (house mouse)	Tysnd1	NP_082188.1
Danio rerio (zebrafish)	tysnd1	NP_001122182.1
Xenopus tropicalis (tropical clawed frog)	tysnd1	XP_002936002.1
Gallus gallus (chicken)	TYSND1	XP_001232633.2
Pan troglodytes (chimpanzee)	TYSND1	XP_001170770.1
	TYSND1	XP_002805742.1
Rattus norvegicus (Norway rat)	Tysnd1	NP_001102402.1

Gene Ontology
Bibliography

Related articles in PubMed

Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.
Mizuno Y, Ninomiya Y, Nakachi Y, Iseki M, Iwasa H, Akita M, Tsukui T, Shimozawa N, Ito C, Toshimori K, Nishimukai M, Hara H, Maeba R, Okazaki T, Alodaib AN, Al Amoudi M, Jacob M, Alkuraya FS, Horai Y, Watanabe M, Motegi H, Wakana S, Noda T, Kurochkin IV, Mizuno Y, Sch?nbach C, Okazaki Y
PLoS genetics9(2)e1003286(2013)

Two proteases, trypsin domain-containing 1 (Tysnd1) and peroxisomal lon protease (PsLon), cooperatively regulate fatty acid ?-oxidation in peroxisomal matrix.
Okumoto K, Kametani Y, Fujiki Y
The Journal of biological chemistry286(52)44367-79(2011 Dec)

Novel peroxisomal protease Tysnd1 processes PTS1- and PTS2-containing enzymes involved in beta-oxidation of fatty acids.
Kurochkin IV, Mizuno Y, Konagaya A, Sakaki Y, Sch?nbach C, Okazaki Y
The EMBO journal26(3)835-45(2007 Feb)

Sequence-based discovery of the human and rodent peroxisomal proteome.
Kurochkin IV, Nagashima T, Konagaya A, Sch?nbach C
Applied bioinformatics4(2)93-104(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data established that peroxisomal processing protease Tysnd1 is necessary to mediate the physiological functions of PTS2-containing substrates.
Title: Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.

characterization of function of human TYSND1, including putative regulation by self-cleavage
Title: Two proteases, trypsin domain-containing 1 (Tysnd1) and peroxisomal lon protease (PsLon), cooperatively regulate fatty acid ?-oxidation in peroxisomal matrix.

The following Tysnd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tysnd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04771	NM_027912.1 Latest version!	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu07487	NM_001272090.1 Latest version!	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu07491	NM_001272091.1 Latest version!	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OMu07492	NM_001272092.1 Latest version!	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu44854	XM_006514168.3 Latest version!	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tysnd1 ( NM_027912.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082188.1 Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu04771
Clone ID Related Accession (Same CDS sequence)	NM_027912.1
Accession Version	NM_027912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	peroxisomal leader peptide-processing protease isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK005069.1, AV148467.1 and AC153136.3. On or before Jul 25, 2009 this sequence version replaced XM_905996.2, XM_125636.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK154513.1, AK005069.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AAGAATGTGG CAATCTGGGG ACAAGTGGTG 
TTTGCCACTC AGGAGACCTC TCCATATGAC ATAGCAGTGG TGAGCCTGGA AGAGGAGCTG 
AATGGTGTTC CCACGCCTGT CCCTGCTGGG CACTTCCATG AAGGCGAGCC TGTCAGCGTG 
GTGGGCTTTG GTGTCTTTGG CCAGGCCTGC GGGCCCTCGG TGACCTCAGG CATCCTGTCG 
GCCGTGGTGC GTGTGGATGG CAGCCCGGTG ATGCTACAGA CCACGTGCGC TGTGCATGGC 
GGCTCCAGCG GAGGACCTCT CTTCTCCTCG GGCTCCGGGG ACCTCCTGGG CATTGTCGCC 
AGCAACACTC GAGACAACAA CACGGGAGCC ACCTACCCAC ACCTGAACTT CAGCATCCCC 
ATCACGGTGC TCCAGCCAGC CCTGAAGCAG TACAGTCAGA CTGGTGACCT GGGTGGCCTC 
CGAGAGCTGG ACCACACCAC TGAGCCGGTC AGGGTCGTGT GGCGGCTGCA GCGGCCCCTG 
TCCGAAGTCC CACGGAGCAA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082188.1
CDS	58..1764
Translation	MGRQWGPSMRVAEQAGCVVSASRAGQPDAGSWSCSGVILSRNPGLVLCHGGIFTPFLRTGSAALTQTGTAFLPGDSCSDDLRLHVQWGPTAASPAGRADQELPGLCTPQCASLGLEPGAPSRARARPLQPPRPAQLLLLLSCPAFRSHFARLFGADAVDQWHFVSSAPDDAVSEEEEEDQLRALGWFALLRVQRGAAAEERRGPVVTVAPLGAVVKGAPLLACGSPFGAFCPDIFLNTLSRGVLSNAAGPLLLTDARCLPGTEGGGVFAARPAGALVALVAAPLCWKAREWVGLTLLCAAAPLLQVARWALARLHPGSASLSVLLPPPDVSTPRGLPLRDLGPPWAAAAVLVECGTVWGSGVVVAPRLVVTCRHVAPREAARVLVHSATPKNVAIWGQVVFATQETSPYDIAVVSLEEELNGVPTPVPAGHFHEGEPVSVVGFGVFGQACGPSVTSGILSAVVRVDGSPVMLQTTCAVHGGSSGGPLFSSGSGDLLGIVASNTRDNNTGATYPHLNFSIPITVLQPALKQYSQTGDLGGLRELDHTTEPVRVVWRLQRPLSEVPRSKL

Target ORF information:

RefSeq Version	NM_027912.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027912.1

ORF Insert Sequence:

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AAGAATGTGG CAATCTGGGG ACAAGTGGTG 
TTTGCCACTC AGGAGACCTC TCCATATGAC ATAGCAGTGG TGAGCCTGGA AGAGGAGCTG 
AATGGTGTTC CCACGCCTGT CCCTGCTGGG CACTTCCATG AAGGCGAGCC TGTCAGCGTG 
GTGGGCTTTG GTGTCTTTGG CCAGGCCTGC GGGCCCTCGG TGACCTCAGG CATCCTGTCG 
GCCGTGGTGC GTGTGGATGG CAGCCCGGTG ATGCTACAGA CCACGTGCGC TGTGCATGGC 
GGCTCCAGCG GAGGACCTCT CTTCTCCTCG GGCTCCGGGG ACCTCCTGGG CATTGTCGCC 
AGCAACACTC GAGACAACAA CACGGGAGCC ACCTACCCAC ACCTGAACTT CAGCATCCCC 
ATCACGGTGC TCCAGCCAGC CCTGAAGCAG TACAGTCAGA CTGGTGACCT GGGTGGCCTC 
CGAGAGCTGG ACCACACCAC TGAGCCGGTC AGGGTCGTGT GGCGGCTGCA GCGGCCCCTG 
TCCGAAGTCC CACGGAGCAA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tysnd1 ( NM_001272090.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001259019.1 Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07487
Clone ID Related Accession (Same CDS sequence)	NM_001272090.1
Accession Version	NM_001272090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	peroxisomal leader peptide-processing protease isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK005069.1, AI181257.1 and AC153136.3. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b) is shorter and has a distinct C-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AI181257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AAGAATGTGG CAATCTGGGG ACAAGTGGTG 
TTTGCCACTC AGGAGACCTC TCCATATGAC ATAGCAGTGG TGAGCCTGGA AGAGGAGCTG 
AATGGTGTTC CCACGCCTGT CCCTGCTGGG CACTTCCATG AAGGCCTGCG GGCCCTCGGT 
GACCTCAGGC ATCCTGTCGG CCGTGGTGCG TGTGGATGGC AGCCCGGTGA TGCTACAGAC 
CACGTGCGCT GTGCATGGCG GCTCCAGCGG AGGACCTCTC TTCTCCTCGG GCTCCGGGGA 
CCTCCTGGGC ATTGTCGCCA GCAACACTCG AGACAACAAC ACGGGAGCCA CCTACCCACA 
CCTGAACTTC AGCATCCCCA TCACGGTGCT CCAGCCAGCC CTGAAGCAGT ACAGTCAGAC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259019.1
CDS	58..1623
Translation	MGRQWGPSMRVAEQAGCVVSASRAGQPDAGSWSCSGVILSRNPGLVLCHGGIFTPFLRTGSAALTQTGTAFLPGDSCSDDLRLHVQWGPTAASPAGRADQELPGLCTPQCASLGLEPGAPSRARARPLQPPRPAQLLLLLSCPAFRSHFARLFGADAVDQWHFVSSAPDDAVSEEEEEDQLRALGWFALLRVQRGAAAEERRGPVVTVAPLGAVVKGAPLLACGSPFGAFCPDIFLNTLSRGVLSNAAGPLLLTDARCLPGTEGGGVFAARPAGALVALVAAPLCWKAREWVGLTLLCAAAPLLQVARWALARLHPGSASLSVLLPPPDVSTPRGLPLRDLGPPWAAAAVLVECGTVWGSGVVVAPRLVVTCRHVAPREAARVLVHSATPKNVAIWGQVVFATQETSPYDIAVVSLEEELNGVPTPVPAGHFHEGLRALGDLRHPVGRGACGWQPGDATDHVRCAWRLQRRTSLLLGLRGPPGHCRQQHSRQQHGSHLPTPELQHPHHGAPASPEAVQSDW

Target ORF information:

RefSeq Version	NM_001272090.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272090.1

ORF Insert Sequence:

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AAGAATGTGG CAATCTGGGG ACAAGTGGTG 
TTTGCCACTC AGGAGACCTC TCCATATGAC ATAGCAGTGG TGAGCCTGGA AGAGGAGCTG 
AATGGTGTTC CCACGCCTGT CCCTGCTGGG CACTTCCATG AAGGCCTGCG GGCCCTCGGT 
GACCTCAGGC ATCCTGTCGG CCGTGGTGCG TGTGGATGGC AGCCCGGTGA TGCTACAGAC 
CACGTGCGCT GTGCATGGCG GCTCCAGCGG AGGACCTCTC TTCTCCTCGG GCTCCGGGGA 
CCTCCTGGGC ATTGTCGCCA GCAACACTCG AGACAACAAC ACGGGAGCCA CCTACCCACA 
CCTGAACTTC AGCATCCCCA TCACGGTGCT CCAGCCAGCC CTGAAGCAGT ACAGTCAGAC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tysnd1 ( NM_001272091.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001259020.1 Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu07491
Clone ID Related Accession (Same CDS sequence)	NM_001272091.1
Accession Version	NM_001272091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	peroxisomal leader peptide-processing protease isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK005069.1 and AC153136.3. Transcript Variant: This variant (3) lacks an alternate exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (c) is shorter and has a distinct C-terminus, compared to isoform a. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AAGCGAGCCT GTCAGCGTGG TGGGCTTTGG 
TGTCTTTGGC CAGGCCTGCG GGCCCTCGGT GACCTCAGGC ATCCTGTCGG CCGTGGTGCG 
TGTGGATGGC AGCCCGGTGA TGCTACAGAC CACGTGCGCT GTGCATGGCG GCTCCAGCGG 
AGGACCTCTC TTCTCCTCGG GCTCCGGGGA CCTCCTGGGC ATTGTCGCCA GCAACACTCG 
AGACAACAAC ACGGGAGCCA CCTACCCACA CCTGAACTTC AGCATCCCCA TCACGGTGCT 
CCAGCCAGCC CTGAAGCAGT ACAGTCAGAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259020.1
CDS	58..1533
Translation	MGRQWGPSMRVAEQAGCVVSASRAGQPDAGSWSCSGVILSRNPGLVLCHGGIFTPFLRTGSAALTQTGTAFLPGDSCSDDLRLHVQWGPTAASPAGRADQELPGLCTPQCASLGLEPGAPSRARARPLQPPRPAQLLLLLSCPAFRSHFARLFGADAVDQWHFVSSAPDDAVSEEEEEDQLRALGWFALLRVQRGAAAEERRGPVVTVAPLGAVVKGAPLLACGSPFGAFCPDIFLNTLSRGVLSNAAGPLLLTDARCLPGTEGGGVFAARPAGALVALVAAPLCWKAREWVGLTLLCAAAPLLQVARWALARLHPGSASLSVLLPPPDVSTPRGLPLRDLGPPWAAAAVLVECGTVWGSGVVVAPRLVVTCRHVAPREAARVLVHSATPKRACQRGGLWCLWPGLRALGDLRHPVGRGACGWQPGDATDHVRCAWRLQRRTSLLLGLRGPPGHCRQQHSRQQHGSHLPTPELQHPHHGAPASPEAVQSDW

Target ORF information:

RefSeq Version	NM_001272091.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272091.1

ORF Insert Sequence:

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AAGCGAGCCT GTCAGCGTGG TGGGCTTTGG 
TGTCTTTGGC CAGGCCTGCG GGCCCTCGGT GACCTCAGGC ATCCTGTCGG CCGTGGTGCG 
TGTGGATGGC AGCCCGGTGA TGCTACAGAC CACGTGCGCT GTGCATGGCG GCTCCAGCGG 
AGGACCTCTC TTCTCCTCGG GCTCCGGGGA CCTCCTGGGC ATTGTCGCCA GCAACACTCG 
AGACAACAAC ACGGGAGCCA CCTACCCACA CCTGAACTTC AGCATCCCCA TCACGGTGCT 
CCAGCCAGCC CTGAAGCAGT ACAGTCAGAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tysnd1 ( NM_001272092.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001259021.1 Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 4, mRNA.

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CloneID	OMu07492
Clone ID Related Accession (Same CDS sequence)	NM_001272092.1
Accession Version	NM_001272092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	peroxisomal leader peptide-processing protease isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK005069.1 and AC153136.3. Transcript Variant: This variant (4) lacks an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (d) is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: BI988268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AAGAATGTGG CAATCTGGGG ACAAGTGGTG 
TTTGCCACTC AGGAGACCTC TCCATATGAC ATAGCAGTGG TGAGCCTGGA AGAGGAGCTG 
AATGGTGTTC CCACGCCTGT CCCTGCTGGG CACTTCCATG AAGGCATTGT CGCCAGCAAC 
ACTCGAGACA ACAACACGGG AGCCACCTAC CCACACCTGA ACTTCAGCAT CCCCATCACG 
GTGCTCCAGC CAGCCCTGAA GCAGTACAGT CAGACTGGTG ACCTGGGTGG CCTCCGAGAG 
CTGGACCACA CCACTGAGCC GGTCAGGGTC GTGTGGCGGC TGCAGCGGCC CCTGTCCGAA 
GTCCCACGGA GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259021.1
CDS	58..1578
Translation	MGRQWGPSMRVAEQAGCVVSASRAGQPDAGSWSCSGVILSRNPGLVLCHGGIFTPFLRTGSAALTQTGTAFLPGDSCSDDLRLHVQWGPTAASPAGRADQELPGLCTPQCASLGLEPGAPSRARARPLQPPRPAQLLLLLSCPAFRSHFARLFGADAVDQWHFVSSAPDDAVSEEEEEDQLRALGWFALLRVQRGAAAEERRGPVVTVAPLGAVVKGAPLLACGSPFGAFCPDIFLNTLSRGVLSNAAGPLLLTDARCLPGTEGGGVFAARPAGALVALVAAPLCWKAREWVGLTLLCAAAPLLQVARWALARLHPGSASLSVLLPPPDVSTPRGLPLRDLGPPWAAAAVLVECGTVWGSGVVVAPRLVVTCRHVAPREAARVLVHSATPKNVAIWGQVVFATQETSPYDIAVVSLEEELNGVPTPVPAGHFHEGIVASNTRDNNTGATYPHLNFSIPITVLQPALKQYSQTGDLGGLRELDHTTEPVRVVWRLQRPLSEVPRSKL

Target ORF information:

RefSeq Version	NM_001272092.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272092.1

ORF Insert Sequence:

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AAGAATGTGG CAATCTGGGG ACAAGTGGTG 
TTTGCCACTC AGGAGACCTC TCCATATGAC ATAGCAGTGG TGAGCCTGGA AGAGGAGCTG 
AATGGTGTTC CCACGCCTGT CCCTGCTGGG CACTTCCATG AAGGCATTGT CGCCAGCAAC 
ACTCGAGACA ACAACACGGG AGCCACCTAC CCACACCTGA ACTTCAGCAT CCCCATCACG 
GTGCTCCAGC CAGCCCTGAA GCAGTACAGT CAGACTGGTG ACCTGGGTGG CCTCCGAGAG 
CTGGACCACA CCACTGAGCC GGTCAGGGTC GTGTGGCGGC TGCAGCGGCC CCTGTCCGAA 
GTCCCACGGA GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tysnd1 ( XM_006514168.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514231.1 Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant X1, mRNA.

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CloneID	OMu44854
Clone ID Related Accession (Same CDS sequence)	XM_006514168.3
Accession Version	XM_006514168.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	peroxisomal leader peptide-processing protease isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514168.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AATGCTAAGA CAACAGAAGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514231.1
CDS	74..1267
Translation	MGRQWGPSMRVAEQAGCVVSASRAGQPDAGSWSCSGVILSRNPGLVLCHGGIFTPFLRTGSAALTQTGTAFLPGDSCSDDLRLHVQWGPTAASPAGRADQELPGLCTPQCASLGLEPGAPSRARARPLQPPRPAQLLLLLSCPAFRSHFARLFGADAVDQWHFVSSAPDDAVSEEEEEDQLRALGWFALLRVQRGAAAEERRGPVVTVAPLGAVVKGAPLLACGSPFGAFCPDIFLNTLSRGVLSNAAGPLLLTDARCLPGTEGGGVFAARPAGALVALVAAPLCWKAREWVGLTLLCAAAPLLQVARWALARLHPGSASLSVLLPPPDVSTPRGLPLRDLGPPWAAAAVLVECGTVWGSGVVVAPRLVVTCRHVAPREAARVLVHSATPNAKTTEG

Target ORF information:

RefSeq Version	XM_006514168.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus trypsin domain containing 1 (Tysnd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514168.3

ORF Insert Sequence:

ATGGGGCGGC AATGGGGACC CTCTATGAGA GTGGCGGAGC AGGCGGGCTG CGTGGTGAGC 
GCCTCGCGGG CCGGACAGCC TGACGCGGGC TCGTGGAGCT GCAGCGGGGT GATTCTGAGC 
CGTAACCCAG GCCTAGTGCT GTGCCACGGA GGCATCTTTA CCCCCTTCCT GCGGACGGGC 
AGCGCGGCGC TGACCCAGAC GGGCACCGCC TTCCTGCCGG GCGACAGTTG CAGCGATGAC 
CTACGTCTCC ACGTGCAGTG GGGACCCACG GCTGCCAGCC CTGCCGGTCG CGCGGATCAG 
GAACTCCCGG GGCTGTGCAC GCCTCAGTGC GCAAGCCTGG GCCTCGAACC CGGCGCCCCG 
AGCCGGGCCC GCGCGCGGCC TCTGCAGCCC CCGCGACCAG CGCAGCTGCT GTTGCTGCTC 
AGCTGCCCGG CCTTCCGCTC GCATTTCGCT CGGCTCTTCG GGGCCGATGC GGTCGATCAA 
TGGCACTTCG TAAGCTCGGC GCCCGACGAT GCGGTTTCGG AGGAGGAAGA GGAGGACCAG 
CTGCGCGCGC TCGGCTGGTT CGCCCTGCTG CGGGTGCAGC GGGGCGCGGC GGCGGAGGAG 
AGGCGCGGAC CGGTGGTGAC CGTGGCACCG CTCGGGGCCG TGGTCAAGGG CGCACCGCTG 
TTGGCTTGTG GCTCTCCGTT CGGTGCCTTC TGCCCGGACA TCTTCCTCAA CACGCTGAGC 
CGCGGCGTGC TCAGCAACGC GGCCGGGCCG CTGCTGCTCA CCGACGCGCG CTGCCTGCCG 
GGCACGGAAG GCGGCGGAGT GTTCGCCGCT CGACCTGCGG GTGCGCTGGT GGCTCTGGTG 
GCCGCGCCGC TCTGCTGGAA GGCCCGCGAG TGGGTGGGCC TCACGCTGCT GTGCGCCGCC 
GCCCCGCTGC TTCAGGTTGC CCGCTGGGCG CTCGCCCGTC TGCACCCCGG CTCCGCTTCC 
CTGTCTGTGC TGCTGCCGCC ACCCGATGTG AGCACGCCAC GGGGCCTGCC TCTGCGCGAT 
CTCGGACCCC CGTGGGCGGC CGCGGCGGTG CTGGTGGAGT GTGGTACCGT GTGGGGCTCC 
GGAGTGGTCG TGGCGCCTCG CCTGGTGGTC ACCTGTCGCC ATGTGGCACC CCGGGAAGCA 
GCCAGGGTGT TGGTACACTC CGCTACACCC AATGCTAAGA CAACAGAAGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.
PLoS genetics9(2)e1003286(2013)
Mizuno Y,Ninomiya Y,Nakachi Y,Iseki M,Iwasa H,Akita M,Tsukui T,Shimozawa N,Ito C,Toshimori K,Nishimukai M,Hara H,Maeba R,Okazaki T,Alodaib AN,Al Amoudi M,Jacob M,Alkuraya FS,Horai Y,Watanabe M,Motegi H,Wakana S,Noda T,Kurochkin IV,Mizuno Y,Sch?nbach C,Okazaki Y

Two proteases, trypsin domain-containing 1 (Tysnd1) and peroxisomal lon protease (PsLon), cooperatively regulate fatty acid ?-oxidation in peroxisomal matrix.
The Journal of biological chemistry286(52)44367-79(2011 Dec)
Okumoto K,Kametani Y,Fujiki Y

Novel peroxisomal protease Tysnd1 processes PTS1- and PTS2-containing enzymes involved in beta-oxidation of fatty acids.
The EMBO journal26(3)835-45(2007 Feb)
Kurochkin IV,Mizuno Y,Konagaya A,Sakaki Y,Sch?nbach C,Okazaki Y

Sequence-based discovery of the human and rodent peroxisomal proteome.
Applied bioinformatics4(2)93-104(2005)
Kurochkin IV,Nagashima T,Konagaya A,Sch?nbach C

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Tysnd1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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