Arhgap21 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006497538.3	XP_006497601.1	rho GTPase-activating protein 21 isoform X1
XM_006497543.3	XP_006497606.1	rho GTPase-activating protein 21 isoform X4
XM_011238986.2	XP_011237288.1	rho GTPase-activating protein 21 isoform X7
XM_030252084.1	XP_030107944.1	rho GTPase-activating protein 21 isoform X6
XM_011238986.3	XP_011237288.1	rho GTPase-activating protein 21 isoform X9
XM_006497541.3	XP_006497604.1	rho GTPase-activating protein 21 isoform X2
XM_017319263.1	XP_017174752.1	rho GTPase-activating protein 21 isoform X9
XM_006497538.4	XP_006497601.1	rho GTPase-activating protein 21 isoform X1
XM_006497544.4	XP_006497607.1	rho GTPase-activating protein 21 isoform X5
XM_030252086.1	XP_030107946.1	rho GTPase-activating protein 21 isoform X7
XM_017319262.2	XP_017174751.1	rho GTPase-activating protein 21 isoform X10
NM_001081364.3	NP_001074833.3	rho GTPase-activating protein 21 isoform 2
XM_017319262.1	XP_017174751.1	rho GTPase-activating protein 21 isoform X8
XM_006497540.3	XP_006497603.1	rho GTPase-activating protein 21 isoform X2
XM_006497545.3	XP_006497608.1	rho GTPase-activating protein 21 isoform X6
NM_001128084.2	NP_001121556.2	rho GTPase-activating protein 21 isoform 1
XM_006497539.3	XP_006497602.1	rho GTPase-activating protein 21 isoform X2
XM_006497542.3	XP_006497605.1	rho GTPase-activating protein 21 isoform X3
XM_006497544.3	XP_006497607.1	rho GTPase-activating protein 21 isoform X5
XM_006497539.4	XP_006497602.1	rho GTPase-activating protein 21 isoform X2
XM_006497542.4	XP_006497605.1	rho GTPase-activating protein 21 isoform X3
XM_006497543.4	XP_006497606.1	rho GTPase-activating protein 21 isoform X4
XM_006497545.4	XP_006497608.1	rho GTPase-activating protein 21 isoform X8
XM_017319263.2	XP_017174752.1	rho GTPase-activating protein 21 isoform X11
XM_006497540.4	XP_006497603.1	rho GTPase-activating protein 21 isoform X2
XM_006497541.4	XP_006497604.1	rho GTPase-activating protein 21 isoform X2
XM_030252085.1	XP_030107945.1	rho GTPase-activating protein 21 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu70706	XM_011238986.3 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu80419	XM_017319262.2 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu80420	XM_017319263.2 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu02220	XM_006497545.4 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu21534	XM_006497543.4 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu29195	XM_006497538.4 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu29196	XM_006497540.4 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu29196	XM_006497541.4 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu29196	XM_006497539.4 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu29197	XM_006497542.4 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu29198	XM_006497544.4 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu88010	XM_030252084.1 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu88010	XM_030252085.1 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu88011	XM_030252086.1 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu02220	NM_001081364.3 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu21534	NM_001128084.2 Latest version!	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu70706	XM_011238986.2	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu29195	XM_006497538.3	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu80419	XM_017319262.1	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu29198	XM_006497544.3	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu29196	XM_006497539.3	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu29197	XM_006497542.3	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu21534	XM_006497543.3	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu02220	XM_006497545.3	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu80420	XM_017319263.1	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29196	XM_006497540.3	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu29196	XM_006497541.3	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

Arhgap21 ( XM_011238986.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237288.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu70706
Clone ID Related Accession (Same CDS sequence)	XM_011238986.2 , XM_011238986.3
Accession Version	XM_011238986.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238986.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAATTCAT GACCTGCCTG AGCATCATTT TGAGACGCTC 
AAGTTTCTCT CGGCTCATCT GAAGACAGTG GCAGAGAACT CAGAAAAGAA TAAGATGGAA 
CCCAGAAACC TAGCGATCGT TTTTGGTCCA ACTCTAGTGA GAACATCAGA AGACAACATG 
ACCCACATGG TCACGCACAT GCCTGACCAG TATAAGATCG TAGAGACTCT CATCCAGCAT 
CATGACTGGT TTTTCACAGA AGAAGGTGCC GAAGAGCCTC TTACAGCAGT GCAGGAGGAG 
AACACAGTAG ACTCCCAGCC AGTACCAAAC ATAGATCATT TGCTCACCAA CATTGGAAGG 
ACAGGCGTGC TCCCTGGAGA TGTATCAGAT TCAGCTACTA GTGACTCCGC AAAATCTAAG 
GGTCTCACTG TGGAGTTGGG GCAGCCTTGG AGCTTGTCCT CTTCCTGGCA GCCTACCCGG 
CACTGTGACT ATAGGTGTAT ACCATCACAC CTGGCAGAAC TCTTCTACTT GTACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237288.1
CDS	14167..18486
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGLTVELGQPWSLSSSWQPTRHCDYRCIPSHLAELFYLYI

Target ORF information:

RefSeq Version	XM_011238986.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238986.3

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAATTCAT GACCTGCCTG AGCATCATTT TGAGACGCTC 
AAGTTTCTCT CGGCTCATCT GAAGACAGTG GCAGAGAACT CAGAAAAGAA TAAGATGGAA 
CCCAGAAACC TAGCGATCGT TTTTGGTCCA ACTCTAGTGA GAACATCAGA AGACAACATG 
ACCCACATGG TCACGCACAT GCCTGACCAG TATAAGATCG TAGAGACTCT CATCCAGCAT 
CATGACTGGT TTTTCACAGA AGAAGGTGCC GAAGAGCCTC TTACAGCAGT GCAGGAGGAG 
AACACAGTAG ACTCCCAGCC AGTACCAAAC ATAGATCATT TGCTCACCAA CATTGGAAGG 
ACAGGCGTGC TCCCTGGAGA TGTATCAGAT TCAGCTACTA GTGACTCCGC AAAATCTAAG 
GGTCTCACTG TGGAGTTGGG GCAGCCTTGG AGCTTGTCCT CTTCCTGGCA GCCTACCCGG 
CACTGTGACT ATAGGTGTAT ACCATCACAC CTGGCAGAAC TCTTCTACTT GTACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_017319262.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174751.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu80419
Clone ID Related Accession (Same CDS sequence)	XM_017319262.2 , XM_017319262.1
Accession Version	XM_017319262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGT 
CTCACTGTGG AGTTGGGGCA GCCTTGGAGC TTGTCCTCTT CCTGGCAGCC TACCCGGCAC 
TGTGACTATA GGTGTATACC ATCACACCTG GCAGAACTCT TCTACTTGTA CATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174751.1
CDS	14167..18483
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGLTVELGQPWSLSSSWQPTRHCDYRCIPSHLAELFYLYI

Target ORF information:

RefSeq Version	XM_017319262.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319262.2

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGT 
CTCACTGTGG AGTTGGGGCA GCCTTGGAGC TTGTCCTCTT CCTGGCAGCC TACCCGGCAC 
TGTGACTATA GGTGTATACC ATCACACCTG GCAGAACTCT TCTACTTGTA CATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_017319262.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174751.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu80419
Clone ID Related Accession (Same CDS sequence)	XM_017319262.2 , XM_017319262.1
Accession Version	XM_017319262.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGT 
CTCACTGTGG AGTTGGGGCA GCCTTGGAGC TTGTCCTCTT CCTGGCAGCC TACCCGGCAC 
TGTGACTATA GGTGTATACC ATCACACCTG GCAGAACTCT TCTACTTGTA CATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174751.1
CDS	11405..15721
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGLTVELGQPWSLSSSWQPTRHCDYRCIPSHLAELFYLYI

Target ORF information:

RefSeq Version	XM_017319262.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319262.1

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGT 
CTCACTGTGG AGTTGGGGCA GCCTTGGAGC TTGTCCTCTT CCTGGCAGCC TACCCGGCAC 
TGTGACTATA GGTGTATACC ATCACACCTG GCAGAACTCT TCTACTTGTA CATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_017319263.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174752.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X13, mRNA.

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CloneID	OMu80420
Clone ID Related Accession (Same CDS sequence)	XM_017319263.1 , XM_017319263.2
Accession Version	XM_017319263.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAGTTCCA GATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174752.1
CDS	11405..15223
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLVPDS

Target ORF information:

RefSeq Version	XM_017319263.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319263.1

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAGTTCCA GATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_017319263.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174752.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu80420
Clone ID Related Accession (Same CDS sequence)	XM_017319263.1 , XM_017319263.2
Accession Version	XM_017319263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAGTTCCA GATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174752.1
CDS	14167..17985
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLVPDS

Target ORF information:

RefSeq Version	XM_017319263.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319263.2

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAGTTCCA GATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497545.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497608.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu02220
Clone ID Related Accession (Same CDS sequence)	NM_001081364.3 , XM_006497545.3 , XM_006497545.4
Accession Version	XM_006497545.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497545.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AAAACAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTTA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACATTCTC CAAAGGAGGA GTCCGAGAGG 
AAGCTTCTCA GTAAAGATGA TACCAGTCCC CCTAAAGACA AAGGTACTTG GAGAAGAGGC 
ATTCCAAGCA TCGTGAGGAA GACGTTTGAG AAAAAGCCGG CCGCCACAGG GACCTTTGGA 
GTTAGGCTGG ATGACTGCCC ACCCGCCCAC ACCAACAGGT ACATTCCATT AATCGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGGGGT CTTGAATATA CAGGTATTTA CCGAGTTCCC 
GGCAACAACG CAGCCATTTC AAGCATGCAG GAAGAACTCA ACAAGGGGAT GGCAGACATT 
GACATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAGC TCCCTGAGCC TCTCTTTACG AATGACAAAT ATGCTGACTT CATTGAGGCC 
AATCGCAAAG AGGACCCACT GGATCGGCTA CGAACATTAA AGAGACTAAT TCATGACCTG 
CCTGAGCATC ATTTTGAGAC GCTCAAGTTT CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG 
AACTCAGAAA AGAATAAGAT GGAACCCAGA AACCTAGCGA TCGTTTTTGG TCCAACTCTA 
GTGAGAACAT CAGAAGACAA CATGACCCAC ATGGTCACGC ACATGCCTGA CCAGTATAAG 
ATCGTAGAGA CTCTCATCCA GCATCATGAC TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG 
CCTCTTACAG CAGTGCAGGA GGAGAACACA GTAGACTCCC AGCCAGTACC AAACATAGAT 
CATTTGCTCA CCAACATTGG AAGGACAGGC GTGCTCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CCGCAAAATC TAAGGGCTCC TGGGGATCCG GGAAGGATCA GTATAGCAGG 
GAACTGCTTG TATCCTCCAT CTTCGCAGCT GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG 
AAAGCCCAGC CCAGCAGCTC TGAGGATGAG CTGGACAGTG TGTTCTTTAA GAAGGAGAAT 
ACGGAACAGA GTCACAGTGA GATTAAAGAA GAGTCCAAAA GAGAAAGCGA GACATCAGGT 
AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA AGTAACACTA AGAAAGACTC GGGTACAACG 
AAGGAGGAGA AGAAGATACC GTGGGAAGAA CCATCCCCAC CACACAGCTC CAAACGCAAC 
AGGTCACCAA CCCTCAGCTG TCGCCTGGCC ATGCTGAAAG AGGGCCCCAG ATCACTTCTG 
ACACAGAAGC CCCACTGTGA AGAGACAGGG TCTGACTCAG GCACTCTGCT TAGCACGTCT 
TCCCAGGCTT CGCTCTTGAG GTCCTCCACT AAAAAATCCA CCAGTCCGGA AACCAAGCAC 
AGTGAGTTTT TGTCCATTGC TGGTACCACC ACCTCGGATT ATTCTACCAC ATCATCCACT 
ACCTACTTGA CCAGCCTGGA CTCCAGCCGC CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG 
AGCAAGGGGG ATGAGGCTGA TGACGAGAGG AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG 
GAGACCGACA GTGAGAGTGA GTTCCCCGTG TTTCCCACCA CCCTGACTTC AGACCGGCTG 
TTCCGAGGAA AATTCCAGGA GGTTGCCAGG GTGAGCCGCA GGAACTCCGA AGGCAGTGAG 
GCCAGCTGCA CCGAGGGAAG CCTCACACCG AGTTTAGACA GCCGCAGGCA GCAGTTCAGC 
TCCCATAGAC TGATCGAATG CGACACACTG TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA 
GACAGTGGGA GCCCCGGAGA CACCAGGACT GAGAAAGAAA CTCCTGCCCT GGCCAAGATG 
TTTGACGTCA TGAAGAAAGG CAAGTCGACA GGGAGCCTCC TCACACCCAG CAGAAGTGAA 
TCCGAAAAGC AGGAGGCCAC TTGGAAAACA AAAATAGCAG ATCGTTTAAA ACTGAGACCC 
AGGGCACCCG CAGACGACAT GTTTGGAGTT GGGAATCAAA AACCTACTGC TGAGACAGCG 
AAAAGGAAAA ACATCAAACG CAGACACACG CTTGGAGGCC ACAGAGATGC TACTGAGATC 
AGCGTCCTGA GTTTCTGGAA AGCTCATGAG CAGAGTGCGG ACAAAGAGTC TGAACTTTCG 
GCTGTGAATC GGTTAAAACC CAAATGCTCA GCCCAGGACC TTTCCATCTC AGACTGGCTC 
GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC GACCTTAGCA GAGGCGAAGG CCTCGAGCCC 
CAGGCTGAGA GCCCCAGCGT GCTGGGAACA CCCATCAGCA CCCACTCACC ACCGTCTCAG 
CAGCCCGAAG CAAGGGTTGC TGCTACCAGC ACCTTGGCTT CAACCAGCCA GTCCCCTCTT 
TTCACACCAC CACAGTCACC TGACCAAATA AACAGAGAAA GCTTCCAGAA CATGAGCCAA 
AATGCTAGTT CTACAGCTAA CATCCACCCC CACAAACAGT CTGAAAGTCC AGACACTAAA 
GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497608.1
CDS	486..6323
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497545.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497545.4

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AAAACAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTTA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACATTCTC CAAAGGAGGA GTCCGAGAGG 
AAGCTTCTCA GTAAAGATGA TACCAGTCCC CCTAAAGACA AAGGTACTTG GAGAAGAGGC 
ATTCCAAGCA TCGTGAGGAA GACGTTTGAG AAAAAGCCGG CCGCCACAGG GACCTTTGGA 
GTTAGGCTGG ATGACTGCCC ACCCGCCCAC ACCAACAGGT ACATTCCATT AATCGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGGGGT CTTGAATATA CAGGTATTTA CCGAGTTCCC 
GGCAACAACG CAGCCATTTC AAGCATGCAG GAAGAACTCA ACAAGGGGAT GGCAGACATT 
GACATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAGC TCCCTGAGCC TCTCTTTACG AATGACAAAT ATGCTGACTT CATTGAGGCC 
AATCGCAAAG AGGACCCACT GGATCGGCTA CGAACATTAA AGAGACTAAT TCATGACCTG 
CCTGAGCATC ATTTTGAGAC GCTCAAGTTT CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG 
AACTCAGAAA AGAATAAGAT GGAACCCAGA AACCTAGCGA TCGTTTTTGG TCCAACTCTA 
GTGAGAACAT CAGAAGACAA CATGACCCAC ATGGTCACGC ACATGCCTGA CCAGTATAAG 
ATCGTAGAGA CTCTCATCCA GCATCATGAC TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG 
CCTCTTACAG CAGTGCAGGA GGAGAACACA GTAGACTCCC AGCCAGTACC AAACATAGAT 
CATTTGCTCA CCAACATTGG AAGGACAGGC GTGCTCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CCGCAAAATC TAAGGGCTCC TGGGGATCCG GGAAGGATCA GTATAGCAGG 
GAACTGCTTG TATCCTCCAT CTTCGCAGCT GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG 
AAAGCCCAGC CCAGCAGCTC TGAGGATGAG CTGGACAGTG TGTTCTTTAA GAAGGAGAAT 
ACGGAACAGA GTCACAGTGA GATTAAAGAA GAGTCCAAAA GAGAAAGCGA GACATCAGGT 
AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA AGTAACACTA AGAAAGACTC GGGTACAACG 
AAGGAGGAGA AGAAGATACC GTGGGAAGAA CCATCCCCAC CACACAGCTC CAAACGCAAC 
AGGTCACCAA CCCTCAGCTG TCGCCTGGCC ATGCTGAAAG AGGGCCCCAG ATCACTTCTG 
ACACAGAAGC CCCACTGTGA AGAGACAGGG TCTGACTCAG GCACTCTGCT TAGCACGTCT 
TCCCAGGCTT CGCTCTTGAG GTCCTCCACT AAAAAATCCA CCAGTCCGGA AACCAAGCAC 
AGTGAGTTTT TGTCCATTGC TGGTACCACC ACCTCGGATT ATTCTACCAC ATCATCCACT 
ACCTACTTGA CCAGCCTGGA CTCCAGCCGC CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG 
AGCAAGGGGG ATGAGGCTGA TGACGAGAGG AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG 
GAGACCGACA GTGAGAGTGA GTTCCCCGTG TTTCCCACCA CCCTGACTTC AGACCGGCTG 
TTCCGAGGAA AATTCCAGGA GGTTGCCAGG GTGAGCCGCA GGAACTCCGA AGGCAGTGAG 
GCCAGCTGCA CCGAGGGAAG CCTCACACCG AGTTTAGACA GCCGCAGGCA GCAGTTCAGC 
TCCCATAGAC TGATCGAATG CGACACACTG TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA 
GACAGTGGGA GCCCCGGAGA CACCAGGACT GAGAAAGAAA CTCCTGCCCT GGCCAAGATG 
TTTGACGTCA TGAAGAAAGG CAAGTCGACA GGGAGCCTCC TCACACCCAG CAGAAGTGAA 
TCCGAAAAGC AGGAGGCCAC TTGGAAAACA AAAATAGCAG ATCGTTTAAA ACTGAGACCC 
AGGGCACCCG CAGACGACAT GTTTGGAGTT GGGAATCAAA AACCTACTGC TGAGACAGCG 
AAAAGGAAAA ACATCAAACG CAGACACACG CTTGGAGGCC ACAGAGATGC TACTGAGATC 
AGCGTCCTGA GTTTCTGGAA AGCTCATGAG CAGAGTGCGG ACAAAGAGTC TGAACTTTCG 
GCTGTGAATC GGTTAAAACC CAAATGCTCA GCCCAGGACC TTTCCATCTC AGACTGGCTC 
GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC GACCTTAGCA GAGGCGAAGG CCTCGAGCCC 
CAGGCTGAGA GCCCCAGCGT GCTGGGAACA CCCATCAGCA CCCACTCACC ACCGTCTCAG 
CAGCCCGAAG CAAGGGTTGC TGCTACCAGC ACCTTGGCTT CAACCAGCCA GTCCCCTCTT 
TTCACACCAC CACAGTCACC TGACCAAATA AACAGAGAAA GCTTCCAGAA CATGAGCCAA 
AATGCTAGTT CTACAGCTAA CATCCACCCC CACAAACAGT CTGAAAGTCC AGACACTAAA 
GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497543.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497606.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X6, mRNA.

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CloneID	OMu21534
Clone ID Related Accession (Same CDS sequence)	XM_006497543.3 , NM_001128084.2 , XM_006497543.4
Accession Version	XM_006497543.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497543.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AAAACAGAAC AGTTGCCAAA AACACCTCGC 
CAGAGTCTTA GCATCAGGCA AACTTTGCTT GGTGCTAAAT CAGAGCCAAA GACTCAAAGC 
CCACATTCTC CAAAGGAGGA GTCCGAGAGG AAGCTTCTCA GTAAAGATGA TACCAGTCCC 
CCTAAAGACA AAGGTACTTG GAGAAGAGGC ATTCCAAGCA TCGTGAGGAA GACGTTTGAG 
AAAAAGCCGG CCGCCACAGG GACCTTTGGA GTTAGGCTGG ATGACTGCCC ACCCGCCCAC 
ACCAACAGGT ACATTCCATT AATCGTTGAC ATATGTTGCA AATTAGTTGA AGAAAGGGGT 
CTTGAATATA CAGGTATTTA CCGAGTTCCC GGCAACAACG CAGCCATTTC AAGCATGCAG 
GAAGAACTCA ACAAGGGGAT GGCAGACATT GACATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATAA GCAGTTTACT AAAATCCTTC TTCAGAAAGC TCCCTGAGCC TCTCTTTACG 
AATGACAAAT ATGCTGACTT CATTGAGGCC AATCGCAAAG AGGACCCACT GGATCGGCTA 
CGAACATTAA AGAGACTAAT TCATGACCTG CCTGAGCATC ATTTTGAGAC GCTCAAGTTT 
CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG AACTCAGAAA AGAATAAGAT GGAACCCAGA 
AACCTAGCGA TCGTTTTTGG TCCAACTCTA GTGAGAACAT CAGAAGACAA CATGACCCAC 
ATGGTCACGC ACATGCCTGA CCAGTATAAG ATCGTAGAGA CTCTCATCCA GCATCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG CCTCTTACAG CAGTGCAGGA GGAGAACACA 
GTAGACTCCC AGCCAGTACC AAACATAGAT CATTTGCTCA CCAACATTGG AAGGACAGGC 
GTGCTCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CCGCAAAATC TAAGGGCTCC 
TGGGGATCCG GGAAGGATCA GTATAGCAGG GAACTGCTTG TATCCTCCAT CTTCGCAGCT 
GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG AAAGCCCAGC CCAGCAGCTC TGAGGATGAG 
CTGGACAGTG TGTTCTTTAA GAAGGAGAAT ACGGAACAGA GTCACAGTGA GATTAAAGAA 
GAGTCCAAAA GAGAAAGCGA GACATCAGGT AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA 
AGTAACACTA AGAAAGACTC GGGTACAACG AAGGAGGAGA AGAAGATACC GTGGGAAGAA 
CCATCCCCAC CACACAGCTC CAAACGCAAC AGGTCACCAA CCCTCAGCTG TCGCCTGGCC 
ATGCTGAAAG AGGGCCCCAG ATCACTTCTG ACACAGAAGC CCCACTGTGA AGAGACAGGG 
TCTGACTCAG GCACTCTGCT TAGCACGTCT TCCCAGGCTT CGCTCTTGAG GTCCTCCACT 
AAAAAATCCA CCAGTCCGGA AACCAAGCAC AGTGAGTTTT TGTCCATTGC TGGTACCACC 
ACCTCGGATT ATTCTACCAC ATCATCCACT ACCTACTTGA CCAGCCTGGA CTCCAGCCGC 
CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG AGCAAGGGGG ATGAGGCTGA TGACGAGAGG 
AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG GAGACCGACA GTGAGAGTGA GTTCCCCGTG 
TTTCCCACCA CCCTGACTTC AGACCGGCTG TTCCGAGGAA AATTCCAGGA GGTTGCCAGG 
GTGAGCCGCA GGAACTCCGA AGGCAGTGAG GCCAGCTGCA CCGAGGGAAG CCTCACACCG 
AGTTTAGACA GCCGCAGGCA GCAGTTCAGC TCCCATAGAC TGATCGAATG CGACACACTG 
TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA GACAGTGGGA GCCCCGGAGA CACCAGGACT 
GAGAAAGAAA CTCCTGCCCT GGCCAAGATG TTTGACGTCA TGAAGAAAGG CAAGTCGACA 
GGGAGCCTCC TCACACCCAG CAGAAGTGAA TCCGAAAAGC AGGAGGCCAC TTGGAAAACA 
AAAATAGCAG ATCGTTTAAA ACTGAGACCC AGGGCACCCG CAGACGACAT GTTTGGAGTT 
GGGAATCAAA AACCTACTGC TGAGACAGCG AAAAGGAAAA ACATCAAACG CAGACACACG 
CTTGGAGGCC ACAGAGATGC TACTGAGATC AGCGTCCTGA GTTTCTGGAA AGCTCATGAG 
CAGAGTGCGG ACAAAGAGTC TGAACTTTCG GCTGTGAATC GGTTAAAACC CAAATGCTCA 
GCCCAGGACC TTTCCATCTC AGACTGGCTC GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC 
GACCTTAGCA GAGGCGAAGG CCTCGAGCCC CAGGCTGAGA GCCCCAGCGT GCTGGGAACA 
CCCATCAGCA CCCACTCACC ACCGTCTCAG CAGCCCGAAG CAAGGGTTGC TGCTACCAGC 
ACCTTGGCTT CAACCAGCCA GTCCCCTCTT TTCACACCAC CACAGTCACC TGACCAAATA 
AACAGAGAAA GCTTCCAGAA CATGAGCCAA AATGCTAGTT CTACAGCTAA CATCCACCCC 
CACAAACAGT CTGAAAGTCC AGACACTAAA GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497606.1
CDS	486..6353
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497543.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497543.4

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AAAACAGAAC AGTTGCCAAA AACACCTCGC 
CAGAGTCTTA GCATCAGGCA AACTTTGCTT GGTGCTAAAT CAGAGCCAAA GACTCAAAGC 
CCACATTCTC CAAAGGAGGA GTCCGAGAGG AAGCTTCTCA GTAAAGATGA TACCAGTCCC 
CCTAAAGACA AAGGTACTTG GAGAAGAGGC ATTCCAAGCA TCGTGAGGAA GACGTTTGAG 
AAAAAGCCGG CCGCCACAGG GACCTTTGGA GTTAGGCTGG ATGACTGCCC ACCCGCCCAC 
ACCAACAGGT ACATTCCATT AATCGTTGAC ATATGTTGCA AATTAGTTGA AGAAAGGGGT 
CTTGAATATA CAGGTATTTA CCGAGTTCCC GGCAACAACG CAGCCATTTC AAGCATGCAG 
GAAGAACTCA ACAAGGGGAT GGCAGACATT GACATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATAA GCAGTTTACT AAAATCCTTC TTCAGAAAGC TCCCTGAGCC TCTCTTTACG 
AATGACAAAT ATGCTGACTT CATTGAGGCC AATCGCAAAG AGGACCCACT GGATCGGCTA 
CGAACATTAA AGAGACTAAT TCATGACCTG CCTGAGCATC ATTTTGAGAC GCTCAAGTTT 
CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG AACTCAGAAA AGAATAAGAT GGAACCCAGA 
AACCTAGCGA TCGTTTTTGG TCCAACTCTA GTGAGAACAT CAGAAGACAA CATGACCCAC 
ATGGTCACGC ACATGCCTGA CCAGTATAAG ATCGTAGAGA CTCTCATCCA GCATCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG CCTCTTACAG CAGTGCAGGA GGAGAACACA 
GTAGACTCCC AGCCAGTACC AAACATAGAT CATTTGCTCA CCAACATTGG AAGGACAGGC 
GTGCTCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CCGCAAAATC TAAGGGCTCC 
TGGGGATCCG GGAAGGATCA GTATAGCAGG GAACTGCTTG TATCCTCCAT CTTCGCAGCT 
GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG AAAGCCCAGC CCAGCAGCTC TGAGGATGAG 
CTGGACAGTG TGTTCTTTAA GAAGGAGAAT ACGGAACAGA GTCACAGTGA GATTAAAGAA 
GAGTCCAAAA GAGAAAGCGA GACATCAGGT AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA 
AGTAACACTA AGAAAGACTC GGGTACAACG AAGGAGGAGA AGAAGATACC GTGGGAAGAA 
CCATCCCCAC CACACAGCTC CAAACGCAAC AGGTCACCAA CCCTCAGCTG TCGCCTGGCC 
ATGCTGAAAG AGGGCCCCAG ATCACTTCTG ACACAGAAGC CCCACTGTGA AGAGACAGGG 
TCTGACTCAG GCACTCTGCT TAGCACGTCT TCCCAGGCTT CGCTCTTGAG GTCCTCCACT 
AAAAAATCCA CCAGTCCGGA AACCAAGCAC AGTGAGTTTT TGTCCATTGC TGGTACCACC 
ACCTCGGATT ATTCTACCAC ATCATCCACT ACCTACTTGA CCAGCCTGGA CTCCAGCCGC 
CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG AGCAAGGGGG ATGAGGCTGA TGACGAGAGG 
AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG GAGACCGACA GTGAGAGTGA GTTCCCCGTG 
TTTCCCACCA CCCTGACTTC AGACCGGCTG TTCCGAGGAA AATTCCAGGA GGTTGCCAGG 
GTGAGCCGCA GGAACTCCGA AGGCAGTGAG GCCAGCTGCA CCGAGGGAAG CCTCACACCG 
AGTTTAGACA GCCGCAGGCA GCAGTTCAGC TCCCATAGAC TGATCGAATG CGACACACTG 
TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA GACAGTGGGA GCCCCGGAGA CACCAGGACT 
GAGAAAGAAA CTCCTGCCCT GGCCAAGATG TTTGACGTCA TGAAGAAAGG CAAGTCGACA 
GGGAGCCTCC TCACACCCAG CAGAAGTGAA TCCGAAAAGC AGGAGGCCAC TTGGAAAACA 
AAAATAGCAG ATCGTTTAAA ACTGAGACCC AGGGCACCCG CAGACGACAT GTTTGGAGTT 
GGGAATCAAA AACCTACTGC TGAGACAGCG AAAAGGAAAA ACATCAAACG CAGACACACG 
CTTGGAGGCC ACAGAGATGC TACTGAGATC AGCGTCCTGA GTTTCTGGAA AGCTCATGAG 
CAGAGTGCGG ACAAAGAGTC TGAACTTTCG GCTGTGAATC GGTTAAAACC CAAATGCTCA 
GCCCAGGACC TTTCCATCTC AGACTGGCTC GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC 
GACCTTAGCA GAGGCGAAGG CCTCGAGCCC CAGGCTGAGA GCCCCAGCGT GCTGGGAACA 
CCCATCAGCA CCCACTCACC ACCGTCTCAG CAGCCCGAAG CAAGGGTTGC TGCTACCAGC 
ACCTTGGCTT CAACCAGCCA GTCCCCTCTT TTCACACCAC CACAGTCACC TGACCAAATA 
AACAGAGAAA GCTTCCAGAA CATGAGCCAA AATGCTAGTT CTACAGCTAA CATCCACCCC 
CACAAACAGT CTGAAAGTCC AGACACTAAA GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497538.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497601.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X1, mRNA.

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CloneID	OMu29195
Clone ID Related Accession (Same CDS sequence)	XM_006497538.3 , XM_006497538.4
Accession Version	XM_006497538.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497538.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAATTCAT GACCTGCCTG AGCATCATTT TGAGACGCTC 
AAGTTTCTCT CGGCTCATCT GAAGACAGTG GCAGAGAACT CAGAAAAGAA TAAGATGGAA 
CCCAGAAACC TAGCGATCGT TTTTGGTCCA ACTCTAGTGA GAACATCAGA AGACAACATG 
ACCCACATGG TCACGCACAT GCCTGACCAG TATAAGATCG TAGAGACTCT CATCCAGCAT 
CATGACTGGT TTTTCACAGA AGAAGGTGCC GAAGAGCCTC TTACAGCAGT GCAGGAGGAG 
AACACAGTAG ACTCCCAGCC AGTACCAAAC ATAGATCATT TGCTCACCAA CATTGGAAGG 
ACAGGCGTGC TCCCTGGAGA TGTATCAGAT TCAGCTACTA GTGACTCCGC AAAATCTAAG 
GGCTCCTGGG GATCCGGGAA GGATCAGTAT AGCAGGGAAC TGCTTGTATC CTCCATCTTC 
GCAGCTGCCA GTCGCAAGAG GAAAAAGCCG AAAGAGAAAG CCCAGCCCAG CAGCTCTGAG 
GATGAGCTGG ACAGTGTGTT CTTTAAGAAG GAGAATACGG AACAGAGTCA CAGTGAGATT 
AAAGAAGAGT CCAAAAGAGA AAGCGAGACA TCAGGTAGCA AGCAGAGGGT CGTCGTTGCC 
AAAGAAAGTA ACACTAAGAA AGACTCGGGT ACAACGAAGG AGGAGAAGAA GATACCGTGG 
GAAGAACCAT CCCCACCACA CAGCTCCAAA CGCAACAGGT CACCAACCCT CAGCTGTCGC 
CTGGCCATGC TGAAAGAGGG CCCCAGATCA CTTCTGACAC AGAAGCCCCA CTGTGAAGAG 
ACAGGGTCTG ACTCAGGCAC TCTGCTTAGC ACGTCTTCCC AGGCTTCGCT CTTGAGGTCC 
TCCACTAAAA AATCCACCAG TCCGGAAACC AAGCACAGTG AGTTTTTGTC CATTGCTGGT 
ACCACCACCT CGGATTATTC TACCACATCA TCCACTACCT ACTTGACCAG CCTGGACTCC 
AGCCGCCTGA GCCCTGAGGT GCAGTCAGTG GCCGAGAGCA AGGGGGATGA GGCTGATGAC 
GAGAGGAGCG AGCTGGTGAG TGAAGGACGG CCAGTGGAGA CCGACAGTGA GAGTGAGTTC 
CCCGTGTTTC CCACCACCCT GACTTCAGAC CGGCTGTTCC GAGGAAAATT CCAGGAGGTT 
GCCAGGGTGA GCCGCAGGAA CTCCGAAGGC AGTGAGGCCA GCTGCACCGA GGGAAGCCTC 
ACACCGAGTT TAGACAGCCG CAGGCAGCAG TTCAGCTCCC ATAGACTGAT CGAATGCGAC 
ACACTGTCCA GGAAGAAGTC TGCTCGCTTC AAGTCAGACA GTGGGAGCCC CGGAGACACC 
AGGACTGAGA AAGAAACTCC TGCCCTGGCC AAGATGTTTG ACGTCATGAA GAAAGGCAAG 
TCGACAGGGA GCCTCCTCAC ACCCAGCAGA AGTGAATCCG AAAAGCAGGA GGCCACTTGG 
AAAACAAAAA TAGCAGATCG TTTAAAACTG AGACCCAGGG CACCCGCAGA CGACATGTTT 
GGAGTTGGGA ATCAAAAACC TACTGCTGAG ACAGCGAAAA GGAAAAACAT CAAACGCAGA 
CACACGCTTG GAGGCCACAG AGATGCTACT GAGATCAGCG TCCTGAGTTT CTGGAAAGCT 
CATGAGCAGA GTGCGGACAA AGAGTCTGAA CTTTCGGCTG TGAATCGGTT AAAACCCAAA 
TGCTCAGCCC AGGACCTTTC CATCTCAGAC TGGCTCGCCA GGGAGCGCGT CCGCACCAGT 
GCCTCCGACC TTAGCAGAGG CGAAGGCCTC GAGCCCCAGG CTGAGAGCCC CAGCGTGCTG 
GGAACACCCA TCAGCACCCA CTCACCACCG TCTCAGCAGC CCGAAGCAAG GGTTGCTGCT 
ACCAGCACCT TGGCTTCAAC CAGCCAGTCC CCTCTTTTCA CACCACCACA GTCACCTGAC 
CAAATAAACA GAGAAAGCTT CCAGAACATG AGCCAAAATG CTAGTTCTAC AGCTAACATC 
CACCCCCACA AACAGTCTGA AAGTCCAGAC ACTAAAGCAG AGACTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497601.1
CDS	14167..20040
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497538.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497538.4

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAATTCAT GACCTGCCTG AGCATCATTT TGAGACGCTC 
AAGTTTCTCT CGGCTCATCT GAAGACAGTG GCAGAGAACT CAGAAAAGAA TAAGATGGAA 
CCCAGAAACC TAGCGATCGT TTTTGGTCCA ACTCTAGTGA GAACATCAGA AGACAACATG 
ACCCACATGG TCACGCACAT GCCTGACCAG TATAAGATCG TAGAGACTCT CATCCAGCAT 
CATGACTGGT TTTTCACAGA AGAAGGTGCC GAAGAGCCTC TTACAGCAGT GCAGGAGGAG 
AACACAGTAG ACTCCCAGCC AGTACCAAAC ATAGATCATT TGCTCACCAA CATTGGAAGG 
ACAGGCGTGC TCCCTGGAGA TGTATCAGAT TCAGCTACTA GTGACTCCGC AAAATCTAAG 
GGCTCCTGGG GATCCGGGAA GGATCAGTAT AGCAGGGAAC TGCTTGTATC CTCCATCTTC 
GCAGCTGCCA GTCGCAAGAG GAAAAAGCCG AAAGAGAAAG CCCAGCCCAG CAGCTCTGAG 
GATGAGCTGG ACAGTGTGTT CTTTAAGAAG GAGAATACGG AACAGAGTCA CAGTGAGATT 
AAAGAAGAGT CCAAAAGAGA AAGCGAGACA TCAGGTAGCA AGCAGAGGGT CGTCGTTGCC 
AAAGAAAGTA ACACTAAGAA AGACTCGGGT ACAACGAAGG AGGAGAAGAA GATACCGTGG 
GAAGAACCAT CCCCACCACA CAGCTCCAAA CGCAACAGGT CACCAACCCT CAGCTGTCGC 
CTGGCCATGC TGAAAGAGGG CCCCAGATCA CTTCTGACAC AGAAGCCCCA CTGTGAAGAG 
ACAGGGTCTG ACTCAGGCAC TCTGCTTAGC ACGTCTTCCC AGGCTTCGCT CTTGAGGTCC 
TCCACTAAAA AATCCACCAG TCCGGAAACC AAGCACAGTG AGTTTTTGTC CATTGCTGGT 
ACCACCACCT CGGATTATTC TACCACATCA TCCACTACCT ACTTGACCAG CCTGGACTCC 
AGCCGCCTGA GCCCTGAGGT GCAGTCAGTG GCCGAGAGCA AGGGGGATGA GGCTGATGAC 
GAGAGGAGCG AGCTGGTGAG TGAAGGACGG CCAGTGGAGA CCGACAGTGA GAGTGAGTTC 
CCCGTGTTTC CCACCACCCT GACTTCAGAC CGGCTGTTCC GAGGAAAATT CCAGGAGGTT 
GCCAGGGTGA GCCGCAGGAA CTCCGAAGGC AGTGAGGCCA GCTGCACCGA GGGAAGCCTC 
ACACCGAGTT TAGACAGCCG CAGGCAGCAG TTCAGCTCCC ATAGACTGAT CGAATGCGAC 
ACACTGTCCA GGAAGAAGTC TGCTCGCTTC AAGTCAGACA GTGGGAGCCC CGGAGACACC 
AGGACTGAGA AAGAAACTCC TGCCCTGGCC AAGATGTTTG ACGTCATGAA GAAAGGCAAG 
TCGACAGGGA GCCTCCTCAC ACCCAGCAGA AGTGAATCCG AAAAGCAGGA GGCCACTTGG 
AAAACAAAAA TAGCAGATCG TTTAAAACTG AGACCCAGGG CACCCGCAGA CGACATGTTT 
GGAGTTGGGA ATCAAAAACC TACTGCTGAG ACAGCGAAAA GGAAAAACAT CAAACGCAGA 
CACACGCTTG GAGGCCACAG AGATGCTACT GAGATCAGCG TCCTGAGTTT CTGGAAAGCT 
CATGAGCAGA GTGCGGACAA AGAGTCTGAA CTTTCGGCTG TGAATCGGTT AAAACCCAAA 
TGCTCAGCCC AGGACCTTTC CATCTCAGAC TGGCTCGCCA GGGAGCGCGT CCGCACCAGT 
GCCTCCGACC TTAGCAGAGG CGAAGGCCTC GAGCCCCAGG CTGAGAGCCC CAGCGTGCTG 
GGAACACCCA TCAGCACCCA CTCACCACCG TCTCAGCAGC CCGAAGCAAG GGTTGCTGCT 
ACCAGCACCT TGGCTTCAAC CAGCCAGTCC CCTCTTTTCA CACCACCACA GTCACCTGAC 
CAAATAAACA GAGAAAGCTT CCAGAACATG AGCCAAAATG CTAGTTCTAC AGCTAACATC 
CACCCCCACA AACAGTCTGA AAGTCCAGAC ACTAAAGCAG AGACTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497539.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497602.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X2, mRNA.

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CloneID	OMu29196
Clone ID Related Accession (Same CDS sequence)	XM_006497541.3 , XM_006497540.3 , XM_006497539.3 , XM_006497539.4 , XM_006497540.4 , XM_006497541.4
Accession Version	XM_006497539.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497539.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497602.1
CDS	486..6356
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497539.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497539.4

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497540.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497603.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X3, mRNA.

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CloneID	OMu29196
Clone ID Related Accession (Same CDS sequence)	XM_006497541.3 , XM_006497540.3 , XM_006497539.3 , XM_006497539.4 , XM_006497540.4 , XM_006497541.4
Accession Version	XM_006497540.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497540.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497603.1
CDS	519..6389
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497540.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497540.4

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497541.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497604.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X5, mRNA.

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CloneID	OMu29196
Clone ID Related Accession (Same CDS sequence)	XM_006497541.3 , XM_006497540.3 , XM_006497539.3 , XM_006497539.4 , XM_006497540.4 , XM_006497541.4
Accession Version	XM_006497541.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497541.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497604.1
CDS	687..6557
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497541.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497541.4

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497542.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497605.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X4, mRNA.

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CloneID	OMu29197
Clone ID Related Accession (Same CDS sequence)	XM_006497542.3 , XM_006497542.4
Accession Version	XM_006497542.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497542.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497605.1
CDS	14167..20037
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497542.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497542.4

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497544.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497607.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X7, mRNA.

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CloneID	OMu29198
Clone ID Related Accession (Same CDS sequence)	XM_006497544.4 , XM_006497544.3
Accession Version	XM_006497544.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497544.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGGCAT ATTCTCAAGA TGCATACCTG 
AAAGGCAATG AAGCTTACAG CGGCAATGCC CGTAATATAC CTGAACCTCC ACCAGTTTGC 
TATCCCTGGT TGCCATCGAC TCCTTCAGCT ACTGCACAGC CGGTGGAAAC ATGCCCTCCT 
GATTCATTAC CGAACAAACA GCAAACCAGT GCACCAGTCC TCACACAACC TGGCAGGGCC 
TATAGAATGG AAATACAAGT GCCTCCGTCA CCAACAGATG TTGCAAAGTC AAACACAGCA 
GTGTGTGTTT GCAATGAGAG TGTGAGGACC GTCATTGTGC CTTCTGAGAA GGTGGTAGAC 
TTGCTAGCCA ATAGAAACAA CCCTTCAGGT CCTTCACATA GAACTGAAGA GGTAAGGTAT 
GGTGTAAATG AGCAGGCCTC TACAAAAGCA GCATCGAGAA CAACGTCACC GGCATCAGTT 
CCCACTGCCC ATCTGATTCA TCAGACCACA GGCTCCAGAT CCTTAGAACC TTCTGGAATT 
TTACTTAAAT CTGGAAATTA CAGTGGACAT TCAGAAGGAA TCTCAAGCAG TAGATCACAA 
GCCGTGGATT CCCCTCCTGT GTCTGTCAAT CACTACTCTG CAAACTCCCA TCAGCACATA 
GACTGGAAAA ATTATAAGAC TTACAAAGAA TACATTGATA ATAGGAGATT GCACATAGGT 
TGTCGGACAA TCCAGGAAAG ACTGGACAGC TTACGAGCTG CATCTCAGAG CGCAGCAGAC 
TATAACCAGG TGGTACCCAC CCGCACTACT CTGCAGGTAC GACGTCGAAG CACCTCTCAT 
GACCGAGTGC CCCAGTCTGT TCAGATCCGG CAGCGCAGTG TATCTCAGGA AAGACTGGAA 
GATTCTGTGC TGATGAAGTA TTGCCCAAGG AGCGCGTCTC AAGGAGCGCT GACCTCACCG 
CCTGTTAGCT TCAATAATCA TAGAACTCGT TCGTGGGATT ACATAGAGGG ACAGACGGAA 
GCCACAGCCA CTGTCAATTC TGAAAGTCAG ATACCTGACT CAAATGGGGA ACGAAAACAG 
ACTTACAAGT GGAGTGGCTT CACTGAGCAG GATGATAGAC GCGGTATTCA CGAAAGACCC 
AGGCAGCAAG AAATGCACAA GCCTTTCCGA GGCTCAAATT TGACTGTGGC TCCCGTTGTT 
AATTCTGATA ACAGGCGCCT GGTTGGGAGA GGGGTAGGAC CTGTCTCACA GTTTAAAAAA 
ATCCCGCCGG ACCTAAGACC ACCACATTCC AACAGAAATT TTCCAACCAC GACTGGGGTG 
TCACTTCAAC GGGGTATTGC ACAAGACAGG TCCCCACTCG TGAAAGTCCG AAGTAATTCT 
CTGAAAGTGC CACCTCCACC TGTGTCAAAA CCGTCCTTCA GCCAGCACTC ACTGGCTTCC 
ATGAAAGACC AAAGGCCTGT CAATCATCTG CATCAACACA GTGTGCTGAG TCAGCAGACG 
CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC CAGCTGGAGA CTGAGGTATC CTCGTGTCTA 
CCAGGAACTT CTGCCAAGAC CAGTCCACAG CTCAGTGAGA ATTTGGGAAC ATCAGACTTA 
GAACTACCTG CCATCCCAAG GAATGGAGAT ATAAACTTAC AGGAGGCTGA AATTCAGCAG 
CCAGATGTGC TAGATAATAA AGAATCTGTC ATTCTAAGAG AGAAACCTCA GTCTGGCCGC 
CAGACGCCAC AGCCTTTAAG ACATCAGTCT TACATCCTGG CAGTGAATGA CCAGGAGACC 
GGGTCAGACA CCACCTGCTG GCTGCCAAAC GATGCACGCA GAGAGGTCCA TATAAAAAGA 
ATGGAGGAGA GGAAAGCTTC AAGCACCAGC CCTCCTGGTG ATTCCCTGGC ATCCATCCCT 
TTTATAGATG AGCCAACCAG TCCTAGCATC GACCACGAGA TTGCACATAT TCCTGCATCT 
GCTGTCATCT CCGCCTCTAC TGCCCACGTC CCCTCCATTG CAACTGTTCC CCCCAGCCTC 
ACGACCTCTG CTCCTTTGAT TCGACGTCAG CTCTCCCACG ACCAAGAATC TGTTGGACCT 
CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG ACAGAAAGAT CCAAATCCTA CGATGAAGGA 
CTGGATGATT ATAGAGAAGA TGCAAAACTA TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA 
ATCAAGATTA CAGACAGCCA GAAGTCATCA GAAGACTCTG GATCGAGAAA GGGTTCTTCC 
TCCGAGGTCT TCAGTGATGC AGCCCGAGAA GGCTGGCTCC AGTTCCGACC ACTGGTCACC 
GATAAGGGCA AGAGAGTTGG TGGCAGTATT CGGCCATGGA AACAGATGTA TGTTGTCCTT 
CGGGGCCACT CACTTTACTT GTACAAAGAC AGAAGAGAGC AGACAACGCC ATCAGAAGAA 
GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG ATAGACATCT CGTACAGTGA GACCAAGAGG 
CGGAATGTAT TCCGACTTAC CACGTCTGAC TGTGAATGCT TGTTCCAGGC TGAAGACAGA 
GATGATATGT TATCATGGAT CAAGACTATT CAGGAAAGTA GCAACCTAAA TGAAGAGGAC 
ACTGGAGTTA CCAACAGAGA TCTAATTAGT CGAAGAATCA AAGAGTATAA CAGTCTGCTG 
AGCAGCAAAA CAGAACAGTT GCCAAAAACA CCTCGCCAGA GTCTTAGCAT CAGGCAAACT 
TTGCTTGGTG CTAAATCAGA GCCAAAGACT CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC 
GAGAGGAAGC TTCTCAGTAA AGATGATACC AGTCCCCCTA AAGACAAAGG TACTTGGAGA 
AGAGGCATTC CAAGCATCGT GAGGAAGACG TTTGAGAAAA AGCCGGCCGC CACAGGGACC 
TTTGGAGTTA GGCTGGATGA CTGCCCACCC GCCCACACCA ACAGGTACAT TCCATTAATC 
GTTGACATAT GTTGCAAATT AGTTGAAGAA AGGGGTCTTG AATATACAGG TATTTACCGA 
GTTCCCGGCA ACAACGCAGC CATTTCAAGC ATGCAGGAAG AACTCAACAA GGGGATGGCA 
GACATTGACA TACAAGATGA TAAATGGCGA GATTTGAATG TGATAAGCAG TTTACTAAAA 
TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC TTTACGAATG ACAAATATGC TGACTTCATT 
GAGGCCAATC GCAAAGAGGA CCCACTGGAT CGGCTACGAA CATTAAAGAG ACTAATTCAT 
GACCTGCCTG AGCATCATTT TGAGACGCTC AAGTTTCTCT CGGCTCATCT GAAGACAGTG 
GCAGAGAACT CAGAAAAGAA TAAGATGGAA CCCAGAAACC TAGCGATCGT TTTTGGTCCA 
ACTCTAGTGA GAACATCAGA AGACAACATG ACCCACATGG TCACGCACAT GCCTGACCAG 
TATAAGATCG TAGAGACTCT CATCCAGCAT CATGACTGGT TTTTCACAGA AGAAGGTGCC 
GAAGAGCCTC TTACAGCAGT GCAGGAGGAG AACACAGTAG ACTCCCAGCC AGTACCAAAC 
ATAGATCATT TGCTCACCAA CATTGGAAGG ACAGGCGTGC TCCCTGGAGA TGTATCAGAT 
TCAGCTACTA GTGACTCCGC AAAATCTAAG GGCTCCTGGG GATCCGGGAA GGATCAGTAT 
AGCAGGGAAC TGCTTGTATC CTCCATCTTC GCAGCTGCCA GTCGCAAGAG GAAAAAGCCG 
AAAGAGAAAG CCCAGCCCAG CAGCTCTGAG GATGAGCTGG ACAGTGTGTT CTTTAAGAAG 
GAGAATACGG AACAGAGTCA CAGTGAGATT AAAGAAGAGT CCAAAAGAGA AAGCGAGACA 
TCAGGTAGCA AGCAGAGGGT CGTCGTTGCC AAAGAAAGTA ACACTAAGAA AGACTCGGGT 
ACAACGAAGG AGGAGAAGAA GATACCGTGG GAAGAACCAT CCCCACCACA CAGCTCCAAA 
CGCAACAGGT CACCAACCCT CAGCTGTCGC CTGGCCATGC TGAAAGAGGG CCCCAGATCA 
CTTCTGACAC AGAAGCCCCA CTGTGAAGAG ACAGGGTCTG ACTCAGGCAC TCTGCTTAGC 
ACGTCTTCCC AGGCTTCGCT CTTGAGGTCC TCCACTAAAA AATCCACCAG TCCGGAAACC 
AAGCACAGTG AGTTTTTGTC CATTGCTGGT ACCACCACCT CGGATTATTC TACCACATCA 
TCCACTACCT ACTTGACCAG CCTGGACTCC AGCCGCCTGA GCCCTGAGGT GCAGTCAGTG 
GCCGAGAGCA AGGGGGATGA GGCTGATGAC GAGAGGAGCG AGCTGGTGAG TGAAGGACGG 
CCAGTGGAGA CCGACAGTGA GAGTGAGTTC CCCGTGTTTC CCACCACCCT GACTTCAGAC 
CGGCTGTTCC GAGGAAAATT CCAGGAGGTT GCCAGGGTGA GCCGCAGGAA CTCCGAAGGC 
AGTGAGGCCA GCTGCACCGA GGGAAGCCTC ACACCGAGTT TAGACAGCCG CAGGCAGCAG 
TTCAGCTCCC ATAGACTGAT CGAATGCGAC ACACTGTCCA GGAAGAAGTC TGCTCGCTTC 
AAGTCAGACA GTGGGAGCCC CGGAGACACC AGGACTGAGA AAGAAACTCC TGCCCTGGCC 
AAGATGTTTG ACGTCATGAA GAAAGGCAAG TCGACAGGGA GCCTCCTCAC ACCCAGCAGA 
AGTGAATCCG AAAAGCAGGA GGCCACTTGG AAAACAAAAA TAGCAGATCG TTTAAAACTG 
AGACCCAGGG CACCCGCAGA CGACATGTTT GGAGTTGGGA ATCAAAAACC TACTGCTGAG 
ACAGCGAAAA GGAAAAACAT CAAACGCAGA CACACGCTTG GAGGCCACAG AGATGCTACT 
GAGATCAGCG TCCTGAGTTT CTGGAAAGCT CATGAGCAGA GTGCGGACAA AGAGTCTGAA 
CTTTCGGCTG TGAATCGGTT AAAACCCAAA TGCTCAGCCC AGGACCTTTC CATCTCAGAC 
TGGCTCGCCA GGGAGCGCGT CCGCACCAGT GCCTCCGACC TTAGCAGAGG CGAAGGCCTC 
GAGCCCCAGG CTGAGAGCCC CAGCGTGCTG GGAACACCCA TCAGCACCCA CTCACCACCG 
TCTCAGCAGC CCGAAGCAAG GGTTGCTGCT ACCAGCACCT TGGCTTCAAC CAGCCAGTCC 
CCTCTTTTCA CACCACCACA GTCACCTGAC CAAATAAACA GAGAAAGCTT CCAGAACATG 
AGCCAAAATG CTAGTTCTAC AGCTAACATC CACCCCCACA AACAGTCTGA AAGTCCAGAC 
ACTAAAGCAG AGACTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497607.1
CDS	14167..20010
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497544.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497544.4

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGGCAT ATTCTCAAGA TGCATACCTG 
AAAGGCAATG AAGCTTACAG CGGCAATGCC CGTAATATAC CTGAACCTCC ACCAGTTTGC 
TATCCCTGGT TGCCATCGAC TCCTTCAGCT ACTGCACAGC CGGTGGAAAC ATGCCCTCCT 
GATTCATTAC CGAACAAACA GCAAACCAGT GCACCAGTCC TCACACAACC TGGCAGGGCC 
TATAGAATGG AAATACAAGT GCCTCCGTCA CCAACAGATG TTGCAAAGTC AAACACAGCA 
GTGTGTGTTT GCAATGAGAG TGTGAGGACC GTCATTGTGC CTTCTGAGAA GGTGGTAGAC 
TTGCTAGCCA ATAGAAACAA CCCTTCAGGT CCTTCACATA GAACTGAAGA GGTAAGGTAT 
GGTGTAAATG AGCAGGCCTC TACAAAAGCA GCATCGAGAA CAACGTCACC GGCATCAGTT 
CCCACTGCCC ATCTGATTCA TCAGACCACA GGCTCCAGAT CCTTAGAACC TTCTGGAATT 
TTACTTAAAT CTGGAAATTA CAGTGGACAT TCAGAAGGAA TCTCAAGCAG TAGATCACAA 
GCCGTGGATT CCCCTCCTGT GTCTGTCAAT CACTACTCTG CAAACTCCCA TCAGCACATA 
GACTGGAAAA ATTATAAGAC TTACAAAGAA TACATTGATA ATAGGAGATT GCACATAGGT 
TGTCGGACAA TCCAGGAAAG ACTGGACAGC TTACGAGCTG CATCTCAGAG CGCAGCAGAC 
TATAACCAGG TGGTACCCAC CCGCACTACT CTGCAGGTAC GACGTCGAAG CACCTCTCAT 
GACCGAGTGC CCCAGTCTGT TCAGATCCGG CAGCGCAGTG TATCTCAGGA AAGACTGGAA 
GATTCTGTGC TGATGAAGTA TTGCCCAAGG AGCGCGTCTC AAGGAGCGCT GACCTCACCG 
CCTGTTAGCT TCAATAATCA TAGAACTCGT TCGTGGGATT ACATAGAGGG ACAGACGGAA 
GCCACAGCCA CTGTCAATTC TGAAAGTCAG ATACCTGACT CAAATGGGGA ACGAAAACAG 
ACTTACAAGT GGAGTGGCTT CACTGAGCAG GATGATAGAC GCGGTATTCA CGAAAGACCC 
AGGCAGCAAG AAATGCACAA GCCTTTCCGA GGCTCAAATT TGACTGTGGC TCCCGTTGTT 
AATTCTGATA ACAGGCGCCT GGTTGGGAGA GGGGTAGGAC CTGTCTCACA GTTTAAAAAA 
ATCCCGCCGG ACCTAAGACC ACCACATTCC AACAGAAATT TTCCAACCAC GACTGGGGTG 
TCACTTCAAC GGGGTATTGC ACAAGACAGG TCCCCACTCG TGAAAGTCCG AAGTAATTCT 
CTGAAAGTGC CACCTCCACC TGTGTCAAAA CCGTCCTTCA GCCAGCACTC ACTGGCTTCC 
ATGAAAGACC AAAGGCCTGT CAATCATCTG CATCAACACA GTGTGCTGAG TCAGCAGACG 
CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC CAGCTGGAGA CTGAGGTATC CTCGTGTCTA 
CCAGGAACTT CTGCCAAGAC CAGTCCACAG CTCAGTGAGA ATTTGGGAAC ATCAGACTTA 
GAACTACCTG CCATCCCAAG GAATGGAGAT ATAAACTTAC AGGAGGCTGA AATTCAGCAG 
CCAGATGTGC TAGATAATAA AGAATCTGTC ATTCTAAGAG AGAAACCTCA GTCTGGCCGC 
CAGACGCCAC AGCCTTTAAG ACATCAGTCT TACATCCTGG CAGTGAATGA CCAGGAGACC 
GGGTCAGACA CCACCTGCTG GCTGCCAAAC GATGCACGCA GAGAGGTCCA TATAAAAAGA 
ATGGAGGAGA GGAAAGCTTC AAGCACCAGC CCTCCTGGTG ATTCCCTGGC ATCCATCCCT 
TTTATAGATG AGCCAACCAG TCCTAGCATC GACCACGAGA TTGCACATAT TCCTGCATCT 
GCTGTCATCT CCGCCTCTAC TGCCCACGTC CCCTCCATTG CAACTGTTCC CCCCAGCCTC 
ACGACCTCTG CTCCTTTGAT TCGACGTCAG CTCTCCCACG ACCAAGAATC TGTTGGACCT 
CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG ACAGAAAGAT CCAAATCCTA CGATGAAGGA 
CTGGATGATT ATAGAGAAGA TGCAAAACTA TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA 
ATCAAGATTA CAGACAGCCA GAAGTCATCA GAAGACTCTG GATCGAGAAA GGGTTCTTCC 
TCCGAGGTCT TCAGTGATGC AGCCCGAGAA GGCTGGCTCC AGTTCCGACC ACTGGTCACC 
GATAAGGGCA AGAGAGTTGG TGGCAGTATT CGGCCATGGA AACAGATGTA TGTTGTCCTT 
CGGGGCCACT CACTTTACTT GTACAAAGAC AGAAGAGAGC AGACAACGCC ATCAGAAGAA 
GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG ATAGACATCT CGTACAGTGA GACCAAGAGG 
CGGAATGTAT TCCGACTTAC CACGTCTGAC TGTGAATGCT TGTTCCAGGC TGAAGACAGA 
GATGATATGT TATCATGGAT CAAGACTATT CAGGAAAGTA GCAACCTAAA TGAAGAGGAC 
ACTGGAGTTA CCAACAGAGA TCTAATTAGT CGAAGAATCA AAGAGTATAA CAGTCTGCTG 
AGCAGCAAAA CAGAACAGTT GCCAAAAACA CCTCGCCAGA GTCTTAGCAT CAGGCAAACT 
TTGCTTGGTG CTAAATCAGA GCCAAAGACT CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC 
GAGAGGAAGC TTCTCAGTAA AGATGATACC AGTCCCCCTA AAGACAAAGG TACTTGGAGA 
AGAGGCATTC CAAGCATCGT GAGGAAGACG TTTGAGAAAA AGCCGGCCGC CACAGGGACC 
TTTGGAGTTA GGCTGGATGA CTGCCCACCC GCCCACACCA ACAGGTACAT TCCATTAATC 
GTTGACATAT GTTGCAAATT AGTTGAAGAA AGGGGTCTTG AATATACAGG TATTTACCGA 
GTTCCCGGCA ACAACGCAGC CATTTCAAGC ATGCAGGAAG AACTCAACAA GGGGATGGCA 
GACATTGACA TACAAGATGA TAAATGGCGA GATTTGAATG TGATAAGCAG TTTACTAAAA 
TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC TTTACGAATG ACAAATATGC TGACTTCATT 
GAGGCCAATC GCAAAGAGGA CCCACTGGAT CGGCTACGAA CATTAAAGAG ACTAATTCAT 
GACCTGCCTG AGCATCATTT TGAGACGCTC AAGTTTCTCT CGGCTCATCT GAAGACAGTG 
GCAGAGAACT CAGAAAAGAA TAAGATGGAA CCCAGAAACC TAGCGATCGT TTTTGGTCCA 
ACTCTAGTGA GAACATCAGA AGACAACATG ACCCACATGG TCACGCACAT GCCTGACCAG 
TATAAGATCG TAGAGACTCT CATCCAGCAT CATGACTGGT TTTTCACAGA AGAAGGTGCC 
GAAGAGCCTC TTACAGCAGT GCAGGAGGAG AACACAGTAG ACTCCCAGCC AGTACCAAAC 
ATAGATCATT TGCTCACCAA CATTGGAAGG ACAGGCGTGC TCCCTGGAGA TGTATCAGAT 
TCAGCTACTA GTGACTCCGC AAAATCTAAG GGCTCCTGGG GATCCGGGAA GGATCAGTAT 
AGCAGGGAAC TGCTTGTATC CTCCATCTTC GCAGCTGCCA GTCGCAAGAG GAAAAAGCCG 
AAAGAGAAAG CCCAGCCCAG CAGCTCTGAG GATGAGCTGG ACAGTGTGTT CTTTAAGAAG 
GAGAATACGG AACAGAGTCA CAGTGAGATT AAAGAAGAGT CCAAAAGAGA AAGCGAGACA 
TCAGGTAGCA AGCAGAGGGT CGTCGTTGCC AAAGAAAGTA ACACTAAGAA AGACTCGGGT 
ACAACGAAGG AGGAGAAGAA GATACCGTGG GAAGAACCAT CCCCACCACA CAGCTCCAAA 
CGCAACAGGT CACCAACCCT CAGCTGTCGC CTGGCCATGC TGAAAGAGGG CCCCAGATCA 
CTTCTGACAC AGAAGCCCCA CTGTGAAGAG ACAGGGTCTG ACTCAGGCAC TCTGCTTAGC 
ACGTCTTCCC AGGCTTCGCT CTTGAGGTCC TCCACTAAAA AATCCACCAG TCCGGAAACC 
AAGCACAGTG AGTTTTTGTC CATTGCTGGT ACCACCACCT CGGATTATTC TACCACATCA 
TCCACTACCT ACTTGACCAG CCTGGACTCC AGCCGCCTGA GCCCTGAGGT GCAGTCAGTG 
GCCGAGAGCA AGGGGGATGA GGCTGATGAC GAGAGGAGCG AGCTGGTGAG TGAAGGACGG 
CCAGTGGAGA CCGACAGTGA GAGTGAGTTC CCCGTGTTTC CCACCACCCT GACTTCAGAC 
CGGCTGTTCC GAGGAAAATT CCAGGAGGTT GCCAGGGTGA GCCGCAGGAA CTCCGAAGGC 
AGTGAGGCCA GCTGCACCGA GGGAAGCCTC ACACCGAGTT TAGACAGCCG CAGGCAGCAG 
TTCAGCTCCC ATAGACTGAT CGAATGCGAC ACACTGTCCA GGAAGAAGTC TGCTCGCTTC 
AAGTCAGACA GTGGGAGCCC CGGAGACACC AGGACTGAGA AAGAAACTCC TGCCCTGGCC 
AAGATGTTTG ACGTCATGAA GAAAGGCAAG TCGACAGGGA GCCTCCTCAC ACCCAGCAGA 
AGTGAATCCG AAAAGCAGGA GGCCACTTGG AAAACAAAAA TAGCAGATCG TTTAAAACTG 
AGACCCAGGG CACCCGCAGA CGACATGTTT GGAGTTGGGA ATCAAAAACC TACTGCTGAG 
ACAGCGAAAA GGAAAAACAT CAAACGCAGA CACACGCTTG GAGGCCACAG AGATGCTACT 
GAGATCAGCG TCCTGAGTTT CTGGAAAGCT CATGAGCAGA GTGCGGACAA AGAGTCTGAA 
CTTTCGGCTG TGAATCGGTT AAAACCCAAA TGCTCAGCCC AGGACCTTTC CATCTCAGAC 
TGGCTCGCCA GGGAGCGCGT CCGCACCAGT GCCTCCGACC TTAGCAGAGG CGAAGGCCTC 
GAGCCCCAGG CTGAGAGCCC CAGCGTGCTG GGAACACCCA TCAGCACCCA CTCACCACCG 
TCTCAGCAGC CCGAAGCAAG GGTTGCTGCT ACCAGCACCT TGGCTTCAAC CAGCCAGTCC 
CCTCTTTTCA CACCACCACA GTCACCTGAC CAAATAAACA GAGAAAGCTT CCAGAACATG 
AGCCAAAATG CTAGTTCTAC AGCTAACATC CACCCCCACA AACAGTCTGA AAGTCCAGAC 
ACTAAAGCAG AGACTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_030252084.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107944.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X8, mRNA.

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CloneID	OMu88010
Clone ID Related Accession (Same CDS sequence)	XM_030252084.1 , XM_030252085.1
Accession Version	XM_030252084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AGCAAAACAG AACAGTTGCC AAAAACACCT CGCCAGAGTC TTAGCATCAG GCAAACTTTG 
CTTGGTGCTA AATCAGAGCC AAAGACTCAA AGCCCACATT CTCCAAAGGA GGAGTCCGAG 
AGGAAGCTTC TCAGTAAAGA TGATACCAGT CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA 
GGCATTCCAA GCATCGTGAG GAAGACGTTT GAGAAAAAGC CGGCCGCCAC AGGGACCTTT 
GGAGTTAGGC TGGATGACTG CCCACCCGCC CACACCAACA GGTACATTCC ATTAATCGTT 
GACATATGTT GCAAATTAGT TGAAGAAAGG GGTCTTGAAT ATACAGGTAT TTACCGAGTT 
CCCGGCAACA ACGCAGCCAT TTCAAGCATG CAGGAAGAAC TCAACAAGGG GATGGCAGAC 
ATTGACATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TAAGCAGTTT ACTAAAATCC 
TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT ACGAATGACA AATATGCTGA CTTCATTGAG 
GCCAATCGCA AAGAGGACCC ACTGGATCGG CTACGAACAT TAAAGAGACT AATTCATGAC 
CTGCCTGAGC ATCATTTTGA GACGCTCAAG TTTCTCTCGG CTCATCTGAA GACAGTGGCA 
GAGAACTCAG AAAAGAATAA GATGGAACCC AGAAACCTAG CGATCGTTTT TGGTCCAACT 
CTAGTGAGAA CATCAGAAGA CAACATGACC CACATGGTCA CGCACATGCC TGACCAGTAT 
AAGATCGTAG AGACTCTCAT CCAGCATCAT GACTGGTTTT TCACAGAAGA AGGTGCCGAA 
GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC ACAGTAGACT CCCAGCCAGT ACCAAACATA 
GATCATTTGC TCACCAACAT TGGAAGGACA GGCGTGCTCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCCGCAAA ATCTAAGGGC TCCTGGGGAT CCGGGAAGGA TCAGTATAGC 
AGGGAACTGC TTGTATCCTC CATCTTCGCA GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA 
GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT GAGCTGGACA GTGTGTTCTT TAAGAAGGAG 
AATACGGAAC AGAGTCACAG TGAGATTAAA GAAGAGTCCA AAAGAGAAAG CGAGACATCA 
GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA GAAAGTAACA CTAAGAAAGA CTCGGGTACA 
ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA GAACCATCCC CACCACACAG CTCCAAACGC 
AACAGGTCAC CAACCCTCAG CTGTCGCCTG GCCATGCTGA AAGAGGGCCC CAGATCACTT 
CTGACACAGA AGCCCCACTG TGAAGAGACA GGGTCTGACT CAGGCACTCT GCTTAGCACG 
TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC ACTAAAAAAT CCACCAGTCC GGAAACCAAG 
CACAGTGAGT TTTTGTCCAT TGCTGGTACC ACCACCTCGG ATTATTCTAC CACATCATCC 
ACTACCTACT TGACCAGCCT GGACTCCAGC CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC 
GAGAGCAAGG GGGATGAGGC TGATGACGAG AGGAGCGAGC TGGTGAGTGA AGGACGGCCA 
GTGGAGACCG ACAGTGAGAG TGAGTTCCCC GTGTTTCCCA CCACCCTGAC TTCAGACCGG 
CTGTTCCGAG GAAAATTCCA GGAGGTTGCC AGGGTGAGCC GCAGGAACTC CGAAGGCAGT 
GAGGCCAGCT GCACCGAGGG AAGCCTCACA CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC 
AGCTCCCATA GACTGATCGA ATGCGACACA CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG 
TCAGACAGTG GGAGCCCCGG AGACACCAGG ACTGAGAAAG AAACTCCTGC CCTGGCCAAG 
ATGTTTGACG TCATGAAGAA AGGCAAGTCG ACAGGGAGCC TCCTCACACC CAGCAGAAGT 
GAATCCGAAA AGCAGGAGGC CACTTGGAAA ACAAAAATAG CAGATCGTTT AAAACTGAGA 
CCCAGGGCAC CCGCAGACGA CATGTTTGGA GTTGGGAATC AAAAACCTAC TGCTGAGACA 
GCGAAAAGGA AAAACATCAA ACGCAGACAC ACGCTTGGAG GCCACAGAGA TGCTACTGAG 
ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT GAGCAGAGTG CGGACAAAGA GTCTGAACTT 
TCGGCTGTGA ATCGGTTAAA ACCCAAATGC TCAGCCCAGG ACCTTTCCAT CTCAGACTGG 
CTCGCCAGGG AGCGCGTCCG CACCAGTGCC TCCGACCTTA GCAGAGGCGA AGGCCTCGAG 
CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA ACACCCATCA GCACCCACTC ACCACCGTCT 
CAGCAGCCCG AAGCAAGGGT TGCTGCTACC AGCACCTTGG CTTCAACCAG CCAGTCCCCT 
CTTTTCACAC CACCACAGTC ACCTGACCAA ATAAACAGAG AAAGCTTCCA GAACATGAGC 
CAAAATGCTA GTTCTACAGC TAACATCCAC CCCCACAAAC AGTCTGAAAG TCCAGACACT 
AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107944.1
CDS	486..6326
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_030252084.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252084.1

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AGCAAAACAG AACAGTTGCC AAAAACACCT CGCCAGAGTC TTAGCATCAG GCAAACTTTG 
CTTGGTGCTA AATCAGAGCC AAAGACTCAA AGCCCACATT CTCCAAAGGA GGAGTCCGAG 
AGGAAGCTTC TCAGTAAAGA TGATACCAGT CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA 
GGCATTCCAA GCATCGTGAG GAAGACGTTT GAGAAAAAGC CGGCCGCCAC AGGGACCTTT 
GGAGTTAGGC TGGATGACTG CCCACCCGCC CACACCAACA GGTACATTCC ATTAATCGTT 
GACATATGTT GCAAATTAGT TGAAGAAAGG GGTCTTGAAT ATACAGGTAT TTACCGAGTT 
CCCGGCAACA ACGCAGCCAT TTCAAGCATG CAGGAAGAAC TCAACAAGGG GATGGCAGAC 
ATTGACATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TAAGCAGTTT ACTAAAATCC 
TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT ACGAATGACA AATATGCTGA CTTCATTGAG 
GCCAATCGCA AAGAGGACCC ACTGGATCGG CTACGAACAT TAAAGAGACT AATTCATGAC 
CTGCCTGAGC ATCATTTTGA GACGCTCAAG TTTCTCTCGG CTCATCTGAA GACAGTGGCA 
GAGAACTCAG AAAAGAATAA GATGGAACCC AGAAACCTAG CGATCGTTTT TGGTCCAACT 
CTAGTGAGAA CATCAGAAGA CAACATGACC CACATGGTCA CGCACATGCC TGACCAGTAT 
AAGATCGTAG AGACTCTCAT CCAGCATCAT GACTGGTTTT TCACAGAAGA AGGTGCCGAA 
GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC ACAGTAGACT CCCAGCCAGT ACCAAACATA 
GATCATTTGC TCACCAACAT TGGAAGGACA GGCGTGCTCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCCGCAAA ATCTAAGGGC TCCTGGGGAT CCGGGAAGGA TCAGTATAGC 
AGGGAACTGC TTGTATCCTC CATCTTCGCA GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA 
GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT GAGCTGGACA GTGTGTTCTT TAAGAAGGAG 
AATACGGAAC AGAGTCACAG TGAGATTAAA GAAGAGTCCA AAAGAGAAAG CGAGACATCA 
GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA GAAAGTAACA CTAAGAAAGA CTCGGGTACA 
ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA GAACCATCCC CACCACACAG CTCCAAACGC 
AACAGGTCAC CAACCCTCAG CTGTCGCCTG GCCATGCTGA AAGAGGGCCC CAGATCACTT 
CTGACACAGA AGCCCCACTG TGAAGAGACA GGGTCTGACT CAGGCACTCT GCTTAGCACG 
TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC ACTAAAAAAT CCACCAGTCC GGAAACCAAG 
CACAGTGAGT TTTTGTCCAT TGCTGGTACC ACCACCTCGG ATTATTCTAC CACATCATCC 
ACTACCTACT TGACCAGCCT GGACTCCAGC CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC 
GAGAGCAAGG GGGATGAGGC TGATGACGAG AGGAGCGAGC TGGTGAGTGA AGGACGGCCA 
GTGGAGACCG ACAGTGAGAG TGAGTTCCCC GTGTTTCCCA CCACCCTGAC TTCAGACCGG 
CTGTTCCGAG GAAAATTCCA GGAGGTTGCC AGGGTGAGCC GCAGGAACTC CGAAGGCAGT 
GAGGCCAGCT GCACCGAGGG AAGCCTCACA CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC 
AGCTCCCATA GACTGATCGA ATGCGACACA CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG 
TCAGACAGTG GGAGCCCCGG AGACACCAGG ACTGAGAAAG AAACTCCTGC CCTGGCCAAG 
ATGTTTGACG TCATGAAGAA AGGCAAGTCG ACAGGGAGCC TCCTCACACC CAGCAGAAGT 
GAATCCGAAA AGCAGGAGGC CACTTGGAAA ACAAAAATAG CAGATCGTTT AAAACTGAGA 
CCCAGGGCAC CCGCAGACGA CATGTTTGGA GTTGGGAATC AAAAACCTAC TGCTGAGACA 
GCGAAAAGGA AAAACATCAA ACGCAGACAC ACGCTTGGAG GCCACAGAGA TGCTACTGAG 
ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT GAGCAGAGTG CGGACAAAGA GTCTGAACTT 
TCGGCTGTGA ATCGGTTAAA ACCCAAATGC TCAGCCCAGG ACCTTTCCAT CTCAGACTGG 
CTCGCCAGGG AGCGCGTCCG CACCAGTGCC TCCGACCTTA GCAGAGGCGA AGGCCTCGAG 
CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA ACACCCATCA GCACCCACTC ACCACCGTCT 
CAGCAGCCCG AAGCAAGGGT TGCTGCTACC AGCACCTTGG CTTCAACCAG CCAGTCCCCT 
CTTTTCACAC CACCACAGTC ACCTGACCAA ATAAACAGAG AAAGCTTCCA GAACATGAGC 
CAAAATGCTA GTTCTACAGC TAACATCCAC CCCCACAAAC AGTCTGAAAG TCCAGACACT 
AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_030252085.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107945.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X9, mRNA.

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CloneID	OMu88010
Clone ID Related Accession (Same CDS sequence)	XM_030252084.1 , XM_030252085.1
Accession Version	XM_030252085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AGCAAAACAG AACAGTTGCC AAAAACACCT CGCCAGAGTC TTAGCATCAG GCAAACTTTG 
CTTGGTGCTA AATCAGAGCC AAAGACTCAA AGCCCACATT CTCCAAAGGA GGAGTCCGAG 
AGGAAGCTTC TCAGTAAAGA TGATACCAGT CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA 
GGCATTCCAA GCATCGTGAG GAAGACGTTT GAGAAAAAGC CGGCCGCCAC AGGGACCTTT 
GGAGTTAGGC TGGATGACTG CCCACCCGCC CACACCAACA GGTACATTCC ATTAATCGTT 
GACATATGTT GCAAATTAGT TGAAGAAAGG GGTCTTGAAT ATACAGGTAT TTACCGAGTT 
CCCGGCAACA ACGCAGCCAT TTCAAGCATG CAGGAAGAAC TCAACAAGGG GATGGCAGAC 
ATTGACATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TAAGCAGTTT ACTAAAATCC 
TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT ACGAATGACA AATATGCTGA CTTCATTGAG 
GCCAATCGCA AAGAGGACCC ACTGGATCGG CTACGAACAT TAAAGAGACT AATTCATGAC 
CTGCCTGAGC ATCATTTTGA GACGCTCAAG TTTCTCTCGG CTCATCTGAA GACAGTGGCA 
GAGAACTCAG AAAAGAATAA GATGGAACCC AGAAACCTAG CGATCGTTTT TGGTCCAACT 
CTAGTGAGAA CATCAGAAGA CAACATGACC CACATGGTCA CGCACATGCC TGACCAGTAT 
AAGATCGTAG AGACTCTCAT CCAGCATCAT GACTGGTTTT TCACAGAAGA AGGTGCCGAA 
GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC ACAGTAGACT CCCAGCCAGT ACCAAACATA 
GATCATTTGC TCACCAACAT TGGAAGGACA GGCGTGCTCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCCGCAAA ATCTAAGGGC TCCTGGGGAT CCGGGAAGGA TCAGTATAGC 
AGGGAACTGC TTGTATCCTC CATCTTCGCA GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA 
GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT GAGCTGGACA GTGTGTTCTT TAAGAAGGAG 
AATACGGAAC AGAGTCACAG TGAGATTAAA GAAGAGTCCA AAAGAGAAAG CGAGACATCA 
GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA GAAAGTAACA CTAAGAAAGA CTCGGGTACA 
ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA GAACCATCCC CACCACACAG CTCCAAACGC 
AACAGGTCAC CAACCCTCAG CTGTCGCCTG GCCATGCTGA AAGAGGGCCC CAGATCACTT 
CTGACACAGA AGCCCCACTG TGAAGAGACA GGGTCTGACT CAGGCACTCT GCTTAGCACG 
TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC ACTAAAAAAT CCACCAGTCC GGAAACCAAG 
CACAGTGAGT TTTTGTCCAT TGCTGGTACC ACCACCTCGG ATTATTCTAC CACATCATCC 
ACTACCTACT TGACCAGCCT GGACTCCAGC CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC 
GAGAGCAAGG GGGATGAGGC TGATGACGAG AGGAGCGAGC TGGTGAGTGA AGGACGGCCA 
GTGGAGACCG ACAGTGAGAG TGAGTTCCCC GTGTTTCCCA CCACCCTGAC TTCAGACCGG 
CTGTTCCGAG GAAAATTCCA GGAGGTTGCC AGGGTGAGCC GCAGGAACTC CGAAGGCAGT 
GAGGCCAGCT GCACCGAGGG AAGCCTCACA CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC 
AGCTCCCATA GACTGATCGA ATGCGACACA CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG 
TCAGACAGTG GGAGCCCCGG AGACACCAGG ACTGAGAAAG AAACTCCTGC CCTGGCCAAG 
ATGTTTGACG TCATGAAGAA AGGCAAGTCG ACAGGGAGCC TCCTCACACC CAGCAGAAGT 
GAATCCGAAA AGCAGGAGGC CACTTGGAAA ACAAAAATAG CAGATCGTTT AAAACTGAGA 
CCCAGGGCAC CCGCAGACGA CATGTTTGGA GTTGGGAATC AAAAACCTAC TGCTGAGACA 
GCGAAAAGGA AAAACATCAA ACGCAGACAC ACGCTTGGAG GCCACAGAGA TGCTACTGAG 
ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT GAGCAGAGTG CGGACAAAGA GTCTGAACTT 
TCGGCTGTGA ATCGGTTAAA ACCCAAATGC TCAGCCCAGG ACCTTTCCAT CTCAGACTGG 
CTCGCCAGGG AGCGCGTCCG CACCAGTGCC TCCGACCTTA GCAGAGGCGA AGGCCTCGAG 
CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA ACACCCATCA GCACCCACTC ACCACCGTCT 
CAGCAGCCCG AAGCAAGGGT TGCTGCTACC AGCACCTTGG CTTCAACCAG CCAGTCCCCT 
CTTTTCACAC CACCACAGTC ACCTGACCAA ATAAACAGAG AAAGCTTCCA GAACATGAGC 
CAAAATGCTA GTTCTACAGC TAACATCCAC CCCCACAAAC AGTCTGAAAG TCCAGACACT 
AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107945.1
CDS	482..6322
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_030252085.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252085.1

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AGCAAAACAG AACAGTTGCC AAAAACACCT CGCCAGAGTC TTAGCATCAG GCAAACTTTG 
CTTGGTGCTA AATCAGAGCC AAAGACTCAA AGCCCACATT CTCCAAAGGA GGAGTCCGAG 
AGGAAGCTTC TCAGTAAAGA TGATACCAGT CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA 
GGCATTCCAA GCATCGTGAG GAAGACGTTT GAGAAAAAGC CGGCCGCCAC AGGGACCTTT 
GGAGTTAGGC TGGATGACTG CCCACCCGCC CACACCAACA GGTACATTCC ATTAATCGTT 
GACATATGTT GCAAATTAGT TGAAGAAAGG GGTCTTGAAT ATACAGGTAT TTACCGAGTT 
CCCGGCAACA ACGCAGCCAT TTCAAGCATG CAGGAAGAAC TCAACAAGGG GATGGCAGAC 
ATTGACATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TAAGCAGTTT ACTAAAATCC 
TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT ACGAATGACA AATATGCTGA CTTCATTGAG 
GCCAATCGCA AAGAGGACCC ACTGGATCGG CTACGAACAT TAAAGAGACT AATTCATGAC 
CTGCCTGAGC ATCATTTTGA GACGCTCAAG TTTCTCTCGG CTCATCTGAA GACAGTGGCA 
GAGAACTCAG AAAAGAATAA GATGGAACCC AGAAACCTAG CGATCGTTTT TGGTCCAACT 
CTAGTGAGAA CATCAGAAGA CAACATGACC CACATGGTCA CGCACATGCC TGACCAGTAT 
AAGATCGTAG AGACTCTCAT CCAGCATCAT GACTGGTTTT TCACAGAAGA AGGTGCCGAA 
GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC ACAGTAGACT CCCAGCCAGT ACCAAACATA 
GATCATTTGC TCACCAACAT TGGAAGGACA GGCGTGCTCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCCGCAAA ATCTAAGGGC TCCTGGGGAT CCGGGAAGGA TCAGTATAGC 
AGGGAACTGC TTGTATCCTC CATCTTCGCA GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA 
GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT GAGCTGGACA GTGTGTTCTT TAAGAAGGAG 
AATACGGAAC AGAGTCACAG TGAGATTAAA GAAGAGTCCA AAAGAGAAAG CGAGACATCA 
GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA GAAAGTAACA CTAAGAAAGA CTCGGGTACA 
ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA GAACCATCCC CACCACACAG CTCCAAACGC 
AACAGGTCAC CAACCCTCAG CTGTCGCCTG GCCATGCTGA AAGAGGGCCC CAGATCACTT 
CTGACACAGA AGCCCCACTG TGAAGAGACA GGGTCTGACT CAGGCACTCT GCTTAGCACG 
TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC ACTAAAAAAT CCACCAGTCC GGAAACCAAG 
CACAGTGAGT TTTTGTCCAT TGCTGGTACC ACCACCTCGG ATTATTCTAC CACATCATCC 
ACTACCTACT TGACCAGCCT GGACTCCAGC CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC 
GAGAGCAAGG GGGATGAGGC TGATGACGAG AGGAGCGAGC TGGTGAGTGA AGGACGGCCA 
GTGGAGACCG ACAGTGAGAG TGAGTTCCCC GTGTTTCCCA CCACCCTGAC TTCAGACCGG 
CTGTTCCGAG GAAAATTCCA GGAGGTTGCC AGGGTGAGCC GCAGGAACTC CGAAGGCAGT 
GAGGCCAGCT GCACCGAGGG AAGCCTCACA CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC 
AGCTCCCATA GACTGATCGA ATGCGACACA CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG 
TCAGACAGTG GGAGCCCCGG AGACACCAGG ACTGAGAAAG AAACTCCTGC CCTGGCCAAG 
ATGTTTGACG TCATGAAGAA AGGCAAGTCG ACAGGGAGCC TCCTCACACC CAGCAGAAGT 
GAATCCGAAA AGCAGGAGGC CACTTGGAAA ACAAAAATAG CAGATCGTTT AAAACTGAGA 
CCCAGGGCAC CCGCAGACGA CATGTTTGGA GTTGGGAATC AAAAACCTAC TGCTGAGACA 
GCGAAAAGGA AAAACATCAA ACGCAGACAC ACGCTTGGAG GCCACAGAGA TGCTACTGAG 
ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT GAGCAGAGTG CGGACAAAGA GTCTGAACTT 
TCGGCTGTGA ATCGGTTAAA ACCCAAATGC TCAGCCCAGG ACCTTTCCAT CTCAGACTGG 
CTCGCCAGGG AGCGCGTCCG CACCAGTGCC TCCGACCTTA GCAGAGGCGA AGGCCTCGAG 
CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA ACACCCATCA GCACCCACTC ACCACCGTCT 
CAGCAGCCCG AAGCAAGGGT TGCTGCTACC AGCACCTTGG CTTCAACCAG CCAGTCCCCT 
CTTTTCACAC CACCACAGTC ACCTGACCAA ATAAACAGAG AAAGCTTCCA GAACATGAGC 
CAAAATGCTA GTTCTACAGC TAACATCCAC CCCCACAAAC AGTCTGAAAG TCCAGACACT 
AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_030252086.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107946.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X10, mRNA.

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CloneID	OMu88011
Clone ID Related Accession (Same CDS sequence)	XM_030252086.1
Accession Version	XM_030252086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGGCAT ATTCTCAAGA TGCATACCTG 
AAAGGCAATG AAGCTTACAG CGGCAATGCC CGTAATATAC CTGAACCTCC ACCAGTTTGC 
TATCCCTGGT TGCCATCGAC TCCTTCAGCT ACTGCACAGC CGGTGGAAAC ATGCCCTCCT 
GATTCATTAC CGAACAAACA GCAAACCAGT GCACCAGTCC TCACACAACC TGGCAGGGCC 
TATAGAATGG AAATACAAGT GCCTCCGTCA CCAACAGATG TTGCAAAGTC AAACACAGCA 
GTGTGTGTTT GCAATGAGAG TGTGAGGACC GTCATTGTGC CTTCTGAGAA GGTGGTAGAC 
TTGCTAGCCA ATAGAAACAA CCCTTCAGGT CCTTCACATA GAACTGAAGA GGTAAGGTAT 
GGTGTAAATG AGCAGGCCTC TACAAAAGCA GCATCGAGAA CAACGTCACC GGCATCAGTT 
CCCACTGCCC ATCTGATTCA TCAGACCACA GGCTCCAGAT CCTTAGAACC TTCTGGAATT 
TTACTTAAAT CTGGAAATTA CAGTGGACAT TCAGAAGGAA TCTCAAGCAG TAGATCACAA 
GCCGTGGATT CCCCTCCTGT GTCTGTCAAT CACTACTCTG CAAACTCCCA TCAGCACATA 
GACTGGAAAA ATTATAAGAC TTACAAAGAA TACATTGATA ATAGGAGATT GCACATAGGT 
TGTCGGACAA TCCAGGAAAG ACTGGACAGC TTACGAGCTG CATCTCAGAG CGCAGCAGAC 
TATAACCAGG TGGTACCCAC CCGCACTACT CTGCAGGTAC GACGTCGAAG CACCTCTCAT 
GACCGAGTGC CCCAGTCTGT TCAGATCCGG CAGCGCAGTG TATCTCAGGA AAGACTGGAA 
GATTCTGTGC TGATGAAGTA TTGCCCAAGG AGCGCGTCTC AAGGAGCGCT GACCTCACCG 
CCTGTTAGCT TCAATAATCA TAGAACTCGT TCGTGGGATT ACATAGAGGG ACAGACGGAA 
GCCACAGCCA CTGTCAATTC TGAAAGTCAG ATACCTGACT CAAATGGGGA ACGAAAACAG 
ACTTACAAGT GGAGTGGCTT CACTGAGCAG GATGATAGAC GCGGTATTCA CGAAAGACCC 
AGGCAGCAAG AAATGCACAA GCCTTTCCGA GGCTCAAATT TGACTGTGGC TCCCGTTGTT 
AATTCTGATA ACAGGCGCCT GGTTGGGAGA GGGGTAGGAC CTGTCTCACA GTTTAAAAAA 
ATCCCGCCGG ACCTAAGACC ACCACATTCC AACAGAAATT TTCCAACCAC GACTGGGGTG 
TCACTTCAAC GGGGTATTGC ACAAGACAGG TCCCCACTCG TGAAAGTCCG AAGTAATTCT 
CTGAAAGTGC CACCTCCACC TGTGTCAAAA CCGTCCTTCA GCCAGCACTC ACTGGCTTCC 
ATGAAAGACC AAAGGCCTGT CAATCATCTG CATCAACACA GTGTGCTGAG TCAGCAGACG 
CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC CAGCTGGAGA CTGAGGTATC CTCGTGTCTA 
CCAGGAACTT CTGCCAAGAC CAGTCCACAG CTCAGTGAGA ATTTGGGAAC ATCAGACTTA 
GAACTACCTG CCATCCCAAG GAATGGAGAT ATAAACTTAC AGGAGGCTGA AATTCAGCAG 
CCAGATGTGC TAGATAATAA AGAATCTGTC ATTCTAAGAG AGAAACCTCA GTCTGGCCGC 
CAGACGCCAC AGCCTTTAAG ACATCAGTCT TACATCCTGG CAGTGAATGA CCAGGAGACC 
GGGTCAGACA CCACCTGCTG GCTGCCAAAC GATGCACGCA GAGAGGTCCA TATAAAAAGA 
ATGGAGGAGA GGAAAGCTTC AAGCACCAGC CCTCCTGGTG ATTCCCTGGC ATCCATCCCT 
TTTATAGATG AGCCAACCAG TCCTAGCATC GACCACGAGA TTGCACATAT TCCTGCATCT 
GCTGTCATCT CCGCCTCTAC TGCCCACGTC CCCTCCATTG CAACTGTTCC CCCCAGCCTC 
ACGACCTCTG CTCCTTTGAT TCGACGTCAG CTCTCCCACG ACCAAGAATC TGTTGGACCT 
CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG ACAGAAAGAT CCAAATCCTA CGATGAAGGA 
CTGGATGATT ATAGAGAAGA TGCAAAACTA TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA 
ATCAAGATTA CAGACAGCCA GAAGTCATCA GAAGACTCTG GATCGAGAAA GGGTTCTTCC 
TCCGAGGTCT TCAGTGATGC AGCCCGAGAA GGCTGGCTCC AGTTCCGACC ACTGGTCACC 
GATAAGGGCA AGAGAGTTGG TGGCAGTATT CGGCCATGGA AACAGATGTA TGTTGTCCTT 
CGGGGCCACT CACTTTACTT GTACAAAGAC AGAAGAGAGC AGACAACGCC ATCAGAAGAA 
GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG ATAGACATCT CGTACAGTGA GACCAAGAGG 
CGGAATGTAT TCCGACTTAC CACGTCTGAC TGTGAATGCT TGTTCCAGGC TGAAGACAGA 
GATGATATGT TATCATGGAT CAAGACTATT CAGGAAAGTA GCAACCTAAA TGAAGAGGAC 
ACTGGAGTTA CCAACAGAGA TCTAATTAGT CGAAGAATCA AAGAGTATAA CAGTCTGCTG 
AGCAAAACAG AACAGTTGCC AAAAACACCT CGCCAGAGTC TTAGCATCAG GCAAACTTTG 
CTTGGTGCTA AATCAGAGCC AAAGACTCAA AGCCCACATT CTCCAAAGGA GGAGTCCGAG 
AGGAAGCTTC TCAGTAAAGA TGATACCAGT CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA 
GGCATTCCAA GCATCGTGAG GAAGACGTTT GAGAAAAAGC CGGCCGCCAC AGGGACCTTT 
GGAGTTAGGC TGGATGACTG CCCACCCGCC CACACCAACA GGTACATTCC ATTAATCGTT 
GACATATGTT GCAAATTAGT TGAAGAAAGG GGTCTTGAAT ATACAGGTAT TTACCGAGTT 
CCCGGCAACA ACGCAGCCAT TTCAAGCATG CAGGAAGAAC TCAACAAGGG GATGGCAGAC 
ATTGACATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TAAGCAGTTT ACTAAAATCC 
TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT ACGAATGACA AATATGCTGA CTTCATTGAG 
GCCAATCGCA AAGAGGACCC ACTGGATCGG CTACGAACAT TAAAGAGACT AATTCATGAC 
CTGCCTGAGC ATCATTTTGA GACGCTCAAG TTTCTCTCGG CTCATCTGAA GACAGTGGCA 
GAGAACTCAG AAAAGAATAA GATGGAACCC AGAAACCTAG CGATCGTTTT TGGTCCAACT 
CTAGTGAGAA CATCAGAAGA CAACATGACC CACATGGTCA CGCACATGCC TGACCAGTAT 
AAGATCGTAG AGACTCTCAT CCAGCATCAT GACTGGTTTT TCACAGAAGA AGGTGCCGAA 
GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC ACAGTAGACT CCCAGCCAGT ACCAAACATA 
GATCATTTGC TCACCAACAT TGGAAGGACA GGCGTGCTCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCCGCAAA ATCTAAGGGC TCCTGGGGAT CCGGGAAGGA TCAGTATAGC 
AGGGAACTGC TTGTATCCTC CATCTTCGCA GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA 
GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT GAGCTGGACA GTGTGTTCTT TAAGAAGGAG 
AATACGGAAC AGAGTCACAG TGAGATTAAA GAAGAGTCCA AAAGAGAAAG CGAGACATCA 
GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA GAAAGTAACA CTAAGAAAGA CTCGGGTACA 
ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA GAACCATCCC CACCACACAG CTCCAAACGC 
AACAGGTCAC CAACCCTCAG CTGTCGCCTG GCCATGCTGA AAGAGGGCCC CAGATCACTT 
CTGACACAGA AGCCCCACTG TGAAGAGACA GGGTCTGACT CAGGCACTCT GCTTAGCACG 
TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC ACTAAAAAAT CCACCAGTCC GGAAACCAAG 
CACAGTGAGT TTTTGTCCAT TGCTGGTACC ACCACCTCGG ATTATTCTAC CACATCATCC 
ACTACCTACT TGACCAGCCT GGACTCCAGC CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC 
GAGAGCAAGG GGGATGAGGC TGATGACGAG AGGAGCGAGC TGGTGAGTGA AGGACGGCCA 
GTGGAGACCG ACAGTGAGAG TGAGTTCCCC GTGTTTCCCA CCACCCTGAC TTCAGACCGG 
CTGTTCCGAG GAAAATTCCA GGAGGTTGCC AGGGTGAGCC GCAGGAACTC CGAAGGCAGT 
GAGGCCAGCT GCACCGAGGG AAGCCTCACA CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC 
AGCTCCCATA GACTGATCGA ATGCGACACA CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG 
TCAGACAGTG GGAGCCCCGG AGACACCAGG ACTGAGAAAG AAACTCCTGC CCTGGCCAAG 
ATGTTTGACG TCATGAAGAA AGGCAAGTCG ACAGGGAGCC TCCTCACACC CAGCAGAAGT 
GAATCCGAAA AGCAGGAGGC CACTTGGAAA ACAAAAATAG CAGATCGTTT AAAACTGAGA 
CCCAGGGCAC CCGCAGACGA CATGTTTGGA GTTGGGAATC AAAAACCTAC TGCTGAGACA 
GCGAAAAGGA AAAACATCAA ACGCAGACAC ACGCTTGGAG GCCACAGAGA TGCTACTGAG 
ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT GAGCAGAGTG CGGACAAAGA GTCTGAACTT 
TCGGCTGTGA ATCGGTTAAA ACCCAAATGC TCAGCCCAGG ACCTTTCCAT CTCAGACTGG 
CTCGCCAGGG AGCGCGTCCG CACCAGTGCC TCCGACCTTA GCAGAGGCGA AGGCCTCGAG 
CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA ACACCCATCA GCACCCACTC ACCACCGTCT 
CAGCAGCCCG AAGCAAGGGT TGCTGCTACC AGCACCTTGG CTTCAACCAG CCAGTCCCCT 
CTTTTCACAC CACCACAGTC ACCTGACCAA ATAAACAGAG AAAGCTTCCA GAACATGAGC 
CAAAATGCTA GTTCTACAGC TAACATCCAC CCCCACAAAC AGTCTGAAAG TCCAGACACT 
AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107946.1
CDS	14167..20007
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_030252086.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252086.1

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGGCAT ATTCTCAAGA TGCATACCTG 
AAAGGCAATG AAGCTTACAG CGGCAATGCC CGTAATATAC CTGAACCTCC ACCAGTTTGC 
TATCCCTGGT TGCCATCGAC TCCTTCAGCT ACTGCACAGC CGGTGGAAAC ATGCCCTCCT 
GATTCATTAC CGAACAAACA GCAAACCAGT GCACCAGTCC TCACACAACC TGGCAGGGCC 
TATAGAATGG AAATACAAGT GCCTCCGTCA CCAACAGATG TTGCAAAGTC AAACACAGCA 
GTGTGTGTTT GCAATGAGAG TGTGAGGACC GTCATTGTGC CTTCTGAGAA GGTGGTAGAC 
TTGCTAGCCA ATAGAAACAA CCCTTCAGGT CCTTCACATA GAACTGAAGA GGTAAGGTAT 
GGTGTAAATG AGCAGGCCTC TACAAAAGCA GCATCGAGAA CAACGTCACC GGCATCAGTT 
CCCACTGCCC ATCTGATTCA TCAGACCACA GGCTCCAGAT CCTTAGAACC TTCTGGAATT 
TTACTTAAAT CTGGAAATTA CAGTGGACAT TCAGAAGGAA TCTCAAGCAG TAGATCACAA 
GCCGTGGATT CCCCTCCTGT GTCTGTCAAT CACTACTCTG CAAACTCCCA TCAGCACATA 
GACTGGAAAA ATTATAAGAC TTACAAAGAA TACATTGATA ATAGGAGATT GCACATAGGT 
TGTCGGACAA TCCAGGAAAG ACTGGACAGC TTACGAGCTG CATCTCAGAG CGCAGCAGAC 
TATAACCAGG TGGTACCCAC CCGCACTACT CTGCAGGTAC GACGTCGAAG CACCTCTCAT 
GACCGAGTGC CCCAGTCTGT TCAGATCCGG CAGCGCAGTG TATCTCAGGA AAGACTGGAA 
GATTCTGTGC TGATGAAGTA TTGCCCAAGG AGCGCGTCTC AAGGAGCGCT GACCTCACCG 
CCTGTTAGCT TCAATAATCA TAGAACTCGT TCGTGGGATT ACATAGAGGG ACAGACGGAA 
GCCACAGCCA CTGTCAATTC TGAAAGTCAG ATACCTGACT CAAATGGGGA ACGAAAACAG 
ACTTACAAGT GGAGTGGCTT CACTGAGCAG GATGATAGAC GCGGTATTCA CGAAAGACCC 
AGGCAGCAAG AAATGCACAA GCCTTTCCGA GGCTCAAATT TGACTGTGGC TCCCGTTGTT 
AATTCTGATA ACAGGCGCCT GGTTGGGAGA GGGGTAGGAC CTGTCTCACA GTTTAAAAAA 
ATCCCGCCGG ACCTAAGACC ACCACATTCC AACAGAAATT TTCCAACCAC GACTGGGGTG 
TCACTTCAAC GGGGTATTGC ACAAGACAGG TCCCCACTCG TGAAAGTCCG AAGTAATTCT 
CTGAAAGTGC CACCTCCACC TGTGTCAAAA CCGTCCTTCA GCCAGCACTC ACTGGCTTCC 
ATGAAAGACC AAAGGCCTGT CAATCATCTG CATCAACACA GTGTGCTGAG TCAGCAGACG 
CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC CAGCTGGAGA CTGAGGTATC CTCGTGTCTA 
CCAGGAACTT CTGCCAAGAC CAGTCCACAG CTCAGTGAGA ATTTGGGAAC ATCAGACTTA 
GAACTACCTG CCATCCCAAG GAATGGAGAT ATAAACTTAC AGGAGGCTGA AATTCAGCAG 
CCAGATGTGC TAGATAATAA AGAATCTGTC ATTCTAAGAG AGAAACCTCA GTCTGGCCGC 
CAGACGCCAC AGCCTTTAAG ACATCAGTCT TACATCCTGG CAGTGAATGA CCAGGAGACC 
GGGTCAGACA CCACCTGCTG GCTGCCAAAC GATGCACGCA GAGAGGTCCA TATAAAAAGA 
ATGGAGGAGA GGAAAGCTTC AAGCACCAGC CCTCCTGGTG ATTCCCTGGC ATCCATCCCT 
TTTATAGATG AGCCAACCAG TCCTAGCATC GACCACGAGA TTGCACATAT TCCTGCATCT 
GCTGTCATCT CCGCCTCTAC TGCCCACGTC CCCTCCATTG CAACTGTTCC CCCCAGCCTC 
ACGACCTCTG CTCCTTTGAT TCGACGTCAG CTCTCCCACG ACCAAGAATC TGTTGGACCT 
CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG ACAGAAAGAT CCAAATCCTA CGATGAAGGA 
CTGGATGATT ATAGAGAAGA TGCAAAACTA TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA 
ATCAAGATTA CAGACAGCCA GAAGTCATCA GAAGACTCTG GATCGAGAAA GGGTTCTTCC 
TCCGAGGTCT TCAGTGATGC AGCCCGAGAA GGCTGGCTCC AGTTCCGACC ACTGGTCACC 
GATAAGGGCA AGAGAGTTGG TGGCAGTATT CGGCCATGGA AACAGATGTA TGTTGTCCTT 
CGGGGCCACT CACTTTACTT GTACAAAGAC AGAAGAGAGC AGACAACGCC ATCAGAAGAA 
GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG ATAGACATCT CGTACAGTGA GACCAAGAGG 
CGGAATGTAT TCCGACTTAC CACGTCTGAC TGTGAATGCT TGTTCCAGGC TGAAGACAGA 
GATGATATGT TATCATGGAT CAAGACTATT CAGGAAAGTA GCAACCTAAA TGAAGAGGAC 
ACTGGAGTTA CCAACAGAGA TCTAATTAGT CGAAGAATCA AAGAGTATAA CAGTCTGCTG 
AGCAAAACAG AACAGTTGCC AAAAACACCT CGCCAGAGTC TTAGCATCAG GCAAACTTTG 
CTTGGTGCTA AATCAGAGCC AAAGACTCAA AGCCCACATT CTCCAAAGGA GGAGTCCGAG 
AGGAAGCTTC TCAGTAAAGA TGATACCAGT CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA 
GGCATTCCAA GCATCGTGAG GAAGACGTTT GAGAAAAAGC CGGCCGCCAC AGGGACCTTT 
GGAGTTAGGC TGGATGACTG CCCACCCGCC CACACCAACA GGTACATTCC ATTAATCGTT 
GACATATGTT GCAAATTAGT TGAAGAAAGG GGTCTTGAAT ATACAGGTAT TTACCGAGTT 
CCCGGCAACA ACGCAGCCAT TTCAAGCATG CAGGAAGAAC TCAACAAGGG GATGGCAGAC 
ATTGACATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TAAGCAGTTT ACTAAAATCC 
TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT ACGAATGACA AATATGCTGA CTTCATTGAG 
GCCAATCGCA AAGAGGACCC ACTGGATCGG CTACGAACAT TAAAGAGACT AATTCATGAC 
CTGCCTGAGC ATCATTTTGA GACGCTCAAG TTTCTCTCGG CTCATCTGAA GACAGTGGCA 
GAGAACTCAG AAAAGAATAA GATGGAACCC AGAAACCTAG CGATCGTTTT TGGTCCAACT 
CTAGTGAGAA CATCAGAAGA CAACATGACC CACATGGTCA CGCACATGCC TGACCAGTAT 
AAGATCGTAG AGACTCTCAT CCAGCATCAT GACTGGTTTT TCACAGAAGA AGGTGCCGAA 
GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC ACAGTAGACT CCCAGCCAGT ACCAAACATA 
GATCATTTGC TCACCAACAT TGGAAGGACA GGCGTGCTCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCCGCAAA ATCTAAGGGC TCCTGGGGAT CCGGGAAGGA TCAGTATAGC 
AGGGAACTGC TTGTATCCTC CATCTTCGCA GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA 
GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT GAGCTGGACA GTGTGTTCTT TAAGAAGGAG 
AATACGGAAC AGAGTCACAG TGAGATTAAA GAAGAGTCCA AAAGAGAAAG CGAGACATCA 
GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA GAAAGTAACA CTAAGAAAGA CTCGGGTACA 
ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA GAACCATCCC CACCACACAG CTCCAAACGC 
AACAGGTCAC CAACCCTCAG CTGTCGCCTG GCCATGCTGA AAGAGGGCCC CAGATCACTT 
CTGACACAGA AGCCCCACTG TGAAGAGACA GGGTCTGACT CAGGCACTCT GCTTAGCACG 
TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC ACTAAAAAAT CCACCAGTCC GGAAACCAAG 
CACAGTGAGT TTTTGTCCAT TGCTGGTACC ACCACCTCGG ATTATTCTAC CACATCATCC 
ACTACCTACT TGACCAGCCT GGACTCCAGC CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC 
GAGAGCAAGG GGGATGAGGC TGATGACGAG AGGAGCGAGC TGGTGAGTGA AGGACGGCCA 
GTGGAGACCG ACAGTGAGAG TGAGTTCCCC GTGTTTCCCA CCACCCTGAC TTCAGACCGG 
CTGTTCCGAG GAAAATTCCA GGAGGTTGCC AGGGTGAGCC GCAGGAACTC CGAAGGCAGT 
GAGGCCAGCT GCACCGAGGG AAGCCTCACA CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC 
AGCTCCCATA GACTGATCGA ATGCGACACA CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG 
TCAGACAGTG GGAGCCCCGG AGACACCAGG ACTGAGAAAG AAACTCCTGC CCTGGCCAAG 
ATGTTTGACG TCATGAAGAA AGGCAAGTCG ACAGGGAGCC TCCTCACACC CAGCAGAAGT 
GAATCCGAAA AGCAGGAGGC CACTTGGAAA ACAAAAATAG CAGATCGTTT AAAACTGAGA 
CCCAGGGCAC CCGCAGACGA CATGTTTGGA GTTGGGAATC AAAAACCTAC TGCTGAGACA 
GCGAAAAGGA AAAACATCAA ACGCAGACAC ACGCTTGGAG GCCACAGAGA TGCTACTGAG 
ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT GAGCAGAGTG CGGACAAAGA GTCTGAACTT 
TCGGCTGTGA ATCGGTTAAA ACCCAAATGC TCAGCCCAGG ACCTTTCCAT CTCAGACTGG 
CTCGCCAGGG AGCGCGTCCG CACCAGTGCC TCCGACCTTA GCAGAGGCGA AGGCCTCGAG 
CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA ACACCCATCA GCACCCACTC ACCACCGTCT 
CAGCAGCCCG AAGCAAGGGT TGCTGCTACC AGCACCTTGG CTTCAACCAG CCAGTCCCCT 
CTTTTCACAC CACCACAGTC ACCTGACCAA ATAAACAGAG AAAGCTTCCA GAACATGAGC 
CAAAATGCTA GTTCTACAGC TAACATCCAC CCCCACAAAC AGTCTGAAAG TCCAGACACT 
AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( NM_001081364.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074833.3 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant 2, mRNA.

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CloneID	OMu02220
Clone ID Related Accession (Same CDS sequence)	NM_001081364.3 , XM_006497545.3 , XM_006497545.4
Accession Version	NM_001081364.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC076629.1, BC043038.1 and BG092259.1. On Sep 17, 2008 this sequence version replaced NM_001081364.2. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. CCDS Note: The coding region has been updated to represent an alternative N-terminus to one that is more supported by available conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC076629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AAAACAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTTA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACATTCTC CAAAGGAGGA GTCCGAGAGG 
AAGCTTCTCA GTAAAGATGA TACCAGTCCC CCTAAAGACA AAGGTACTTG GAGAAGAGGC 
ATTCCAAGCA TCGTGAGGAA GACGTTTGAG AAAAAGCCGG CCGCCACAGG GACCTTTGGA 
GTTAGGCTGG ATGACTGCCC ACCCGCCCAC ACCAACAGGT ACATTCCATT AATCGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGGGGT CTTGAATATA CAGGTATTTA CCGAGTTCCC 
GGCAACAACG CAGCCATTTC AAGCATGCAG GAAGAACTCA ACAAGGGGAT GGCAGACATT 
GACATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAGC TCCCTGAGCC TCTCTTTACG AATGACAAAT ATGCTGACTT CATTGAGGCC 
AATCGCAAAG AGGACCCACT GGATCGGCTA CGAACATTAA AGAGACTAAT TCATGACCTG 
CCTGAGCATC ATTTTGAGAC GCTCAAGTTT CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG 
AACTCAGAAA AGAATAAGAT GGAACCCAGA AACCTAGCGA TCGTTTTTGG TCCAACTCTA 
GTGAGAACAT CAGAAGACAA CATGACCCAC ATGGTCACGC ACATGCCTGA CCAGTATAAG 
ATCGTAGAGA CTCTCATCCA GCATCATGAC TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG 
CCTCTTACAG CAGTGCAGGA GGAGAACACA GTAGACTCCC AGCCAGTACC AAACATAGAT 
CATTTGCTCA CCAACATTGG AAGGACAGGC GTGCTCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CCGCAAAATC TAAGGGCTCC TGGGGATCCG GGAAGGATCA GTATAGCAGG 
GAACTGCTTG TATCCTCCAT CTTCGCAGCT GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG 
AAAGCCCAGC CCAGCAGCTC TGAGGATGAG CTGGACAGTG TGTTCTTTAA GAAGGAGAAT 
ACGGAACAGA GTCACAGTGA GATTAAAGAA GAGTCCAAAA GAGAAAGCGA GACATCAGGT 
AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA AGTAACACTA AGAAAGACTC GGGTACAACG 
AAGGAGGAGA AGAAGATACC GTGGGAAGAA CCATCCCCAC CACACAGCTC CAAACGCAAC 
AGGTCACCAA CCCTCAGCTG TCGCCTGGCC ATGCTGAAAG AGGGCCCCAG ATCACTTCTG 
ACACAGAAGC CCCACTGTGA AGAGACAGGG TCTGACTCAG GCACTCTGCT TAGCACGTCT 
TCCCAGGCTT CGCTCTTGAG GTCCTCCACT AAAAAATCCA CCAGTCCGGA AACCAAGCAC 
AGTGAGTTTT TGTCCATTGC TGGTACCACC ACCTCGGATT ATTCTACCAC ATCATCCACT 
ACCTACTTGA CCAGCCTGGA CTCCAGCCGC CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG 
AGCAAGGGGG ATGAGGCTGA TGACGAGAGG AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG 
GAGACCGACA GTGAGAGTGA GTTCCCCGTG TTTCCCACCA CCCTGACTTC AGACCGGCTG 
TTCCGAGGAA AATTCCAGGA GGTTGCCAGG GTGAGCCGCA GGAACTCCGA AGGCAGTGAG 
GCCAGCTGCA CCGAGGGAAG CCTCACACCG AGTTTAGACA GCCGCAGGCA GCAGTTCAGC 
TCCCATAGAC TGATCGAATG CGACACACTG TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA 
GACAGTGGGA GCCCCGGAGA CACCAGGACT GAGAAAGAAA CTCCTGCCCT GGCCAAGATG 
TTTGACGTCA TGAAGAAAGG CAAGTCGACA GGGAGCCTCC TCACACCCAG CAGAAGTGAA 
TCCGAAAAGC AGGAGGCCAC TTGGAAAACA AAAATAGCAG ATCGTTTAAA ACTGAGACCC 
AGGGCACCCG CAGACGACAT GTTTGGAGTT GGGAATCAAA AACCTACTGC TGAGACAGCG 
AAAAGGAAAA ACATCAAACG CAGACACACG CTTGGAGGCC ACAGAGATGC TACTGAGATC 
AGCGTCCTGA GTTTCTGGAA AGCTCATGAG CAGAGTGCGG ACAAAGAGTC TGAACTTTCG 
GCTGTGAATC GGTTAAAACC CAAATGCTCA GCCCAGGACC TTTCCATCTC AGACTGGCTC 
GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC GACCTTAGCA GAGGCGAAGG CCTCGAGCCC 
CAGGCTGAGA GCCCCAGCGT GCTGGGAACA CCCATCAGCA CCCACTCACC ACCGTCTCAG 
CAGCCCGAAG CAAGGGTTGC TGCTACCAGC ACCTTGGCTT CAACCAGCCA GTCCCCTCTT 
TTCACACCAC CACAGTCACC TGACCAAATA AACAGAGAAA GCTTCCAGAA CATGAGCCAA 
AATGCTAGTT CTACAGCTAA CATCCACCCC CACAAACAGT CTGAAAGTCC AGACACTAAA 
GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074833.3
CDS	312..6149
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	NM_001081364.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081364.3

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AAAACAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTTA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACATTCTC CAAAGGAGGA GTCCGAGAGG 
AAGCTTCTCA GTAAAGATGA TACCAGTCCC CCTAAAGACA AAGGTACTTG GAGAAGAGGC 
ATTCCAAGCA TCGTGAGGAA GACGTTTGAG AAAAAGCCGG CCGCCACAGG GACCTTTGGA 
GTTAGGCTGG ATGACTGCCC ACCCGCCCAC ACCAACAGGT ACATTCCATT AATCGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGGGGT CTTGAATATA CAGGTATTTA CCGAGTTCCC 
GGCAACAACG CAGCCATTTC AAGCATGCAG GAAGAACTCA ACAAGGGGAT GGCAGACATT 
GACATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAGC TCCCTGAGCC TCTCTTTACG AATGACAAAT ATGCTGACTT CATTGAGGCC 
AATCGCAAAG AGGACCCACT GGATCGGCTA CGAACATTAA AGAGACTAAT TCATGACCTG 
CCTGAGCATC ATTTTGAGAC GCTCAAGTTT CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG 
AACTCAGAAA AGAATAAGAT GGAACCCAGA AACCTAGCGA TCGTTTTTGG TCCAACTCTA 
GTGAGAACAT CAGAAGACAA CATGACCCAC ATGGTCACGC ACATGCCTGA CCAGTATAAG 
ATCGTAGAGA CTCTCATCCA GCATCATGAC TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG 
CCTCTTACAG CAGTGCAGGA GGAGAACACA GTAGACTCCC AGCCAGTACC AAACATAGAT 
CATTTGCTCA CCAACATTGG AAGGACAGGC GTGCTCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CCGCAAAATC TAAGGGCTCC TGGGGATCCG GGAAGGATCA GTATAGCAGG 
GAACTGCTTG TATCCTCCAT CTTCGCAGCT GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG 
AAAGCCCAGC CCAGCAGCTC TGAGGATGAG CTGGACAGTG TGTTCTTTAA GAAGGAGAAT 
ACGGAACAGA GTCACAGTGA GATTAAAGAA GAGTCCAAAA GAGAAAGCGA GACATCAGGT 
AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA AGTAACACTA AGAAAGACTC GGGTACAACG 
AAGGAGGAGA AGAAGATACC GTGGGAAGAA CCATCCCCAC CACACAGCTC CAAACGCAAC 
AGGTCACCAA CCCTCAGCTG TCGCCTGGCC ATGCTGAAAG AGGGCCCCAG ATCACTTCTG 
ACACAGAAGC CCCACTGTGA AGAGACAGGG TCTGACTCAG GCACTCTGCT TAGCACGTCT 
TCCCAGGCTT CGCTCTTGAG GTCCTCCACT AAAAAATCCA CCAGTCCGGA AACCAAGCAC 
AGTGAGTTTT TGTCCATTGC TGGTACCACC ACCTCGGATT ATTCTACCAC ATCATCCACT 
ACCTACTTGA CCAGCCTGGA CTCCAGCCGC CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG 
AGCAAGGGGG ATGAGGCTGA TGACGAGAGG AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG 
GAGACCGACA GTGAGAGTGA GTTCCCCGTG TTTCCCACCA CCCTGACTTC AGACCGGCTG 
TTCCGAGGAA AATTCCAGGA GGTTGCCAGG GTGAGCCGCA GGAACTCCGA AGGCAGTGAG 
GCCAGCTGCA CCGAGGGAAG CCTCACACCG AGTTTAGACA GCCGCAGGCA GCAGTTCAGC 
TCCCATAGAC TGATCGAATG CGACACACTG TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA 
GACAGTGGGA GCCCCGGAGA CACCAGGACT GAGAAAGAAA CTCCTGCCCT GGCCAAGATG 
TTTGACGTCA TGAAGAAAGG CAAGTCGACA GGGAGCCTCC TCACACCCAG CAGAAGTGAA 
TCCGAAAAGC AGGAGGCCAC TTGGAAAACA AAAATAGCAG ATCGTTTAAA ACTGAGACCC 
AGGGCACCCG CAGACGACAT GTTTGGAGTT GGGAATCAAA AACCTACTGC TGAGACAGCG 
AAAAGGAAAA ACATCAAACG CAGACACACG CTTGGAGGCC ACAGAGATGC TACTGAGATC 
AGCGTCCTGA GTTTCTGGAA AGCTCATGAG CAGAGTGCGG ACAAAGAGTC TGAACTTTCG 
GCTGTGAATC GGTTAAAACC CAAATGCTCA GCCCAGGACC TTTCCATCTC AGACTGGCTC 
GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC GACCTTAGCA GAGGCGAAGG CCTCGAGCCC 
CAGGCTGAGA GCCCCAGCGT GCTGGGAACA CCCATCAGCA CCCACTCACC ACCGTCTCAG 
CAGCCCGAAG CAAGGGTTGC TGCTACCAGC ACCTTGGCTT CAACCAGCCA GTCCCCTCTT 
TTCACACCAC CACAGTCACC TGACCAAATA AACAGAGAAA GCTTCCAGAA CATGAGCCAA 
AATGCTAGTT CTACAGCTAA CATCCACCCC CACAAACAGT CTGAAAGTCC AGACACTAAA 
GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( NM_001128084.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001121556.2 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant 1, mRNA.

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CloneID	OMu21534
Clone ID Related Accession (Same CDS sequence)	XM_006497543.3 , NM_001128084.2 , XM_006497543.4
Accession Version	NM_001128084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL773540.18, BX649226.1, AL929100.11, BC058126.1 and BG092259.1. On Sep 17, 2008 this sequence version replaced NM_001128084.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was creaed from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150741.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AAAACAGAAC AGTTGCCAAA AACACCTCGC 
CAGAGTCTTA GCATCAGGCA AACTTTGCTT GGTGCTAAAT CAGAGCCAAA GACTCAAAGC 
CCACATTCTC CAAAGGAGGA GTCCGAGAGG AAGCTTCTCA GTAAAGATGA TACCAGTCCC 
CCTAAAGACA AAGGTACTTG GAGAAGAGGC ATTCCAAGCA TCGTGAGGAA GACGTTTGAG 
AAAAAGCCGG CCGCCACAGG GACCTTTGGA GTTAGGCTGG ATGACTGCCC ACCCGCCCAC 
ACCAACAGGT ACATTCCATT AATCGTTGAC ATATGTTGCA AATTAGTTGA AGAAAGGGGT 
CTTGAATATA CAGGTATTTA CCGAGTTCCC GGCAACAACG CAGCCATTTC AAGCATGCAG 
GAAGAACTCA ACAAGGGGAT GGCAGACATT GACATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATAA GCAGTTTACT AAAATCCTTC TTCAGAAAGC TCCCTGAGCC TCTCTTTACG 
AATGACAAAT ATGCTGACTT CATTGAGGCC AATCGCAAAG AGGACCCACT GGATCGGCTA 
CGAACATTAA AGAGACTAAT TCATGACCTG CCTGAGCATC ATTTTGAGAC GCTCAAGTTT 
CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG AACTCAGAAA AGAATAAGAT GGAACCCAGA 
AACCTAGCGA TCGTTTTTGG TCCAACTCTA GTGAGAACAT CAGAAGACAA CATGACCCAC 
ATGGTCACGC ACATGCCTGA CCAGTATAAG ATCGTAGAGA CTCTCATCCA GCATCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG CCTCTTACAG CAGTGCAGGA GGAGAACACA 
GTAGACTCCC AGCCAGTACC AAACATAGAT CATTTGCTCA CCAACATTGG AAGGACAGGC 
GTGCTCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CCGCAAAATC TAAGGGCTCC 
TGGGGATCCG GGAAGGATCA GTATAGCAGG GAACTGCTTG TATCCTCCAT CTTCGCAGCT 
GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG AAAGCCCAGC CCAGCAGCTC TGAGGATGAG 
CTGGACAGTG TGTTCTTTAA GAAGGAGAAT ACGGAACAGA GTCACAGTGA GATTAAAGAA 
GAGTCCAAAA GAGAAAGCGA GACATCAGGT AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA 
AGTAACACTA AGAAAGACTC GGGTACAACG AAGGAGGAGA AGAAGATACC GTGGGAAGAA 
CCATCCCCAC CACACAGCTC CAAACGCAAC AGGTCACCAA CCCTCAGCTG TCGCCTGGCC 
ATGCTGAAAG AGGGCCCCAG ATCACTTCTG ACACAGAAGC CCCACTGTGA AGAGACAGGG 
TCTGACTCAG GCACTCTGCT TAGCACGTCT TCCCAGGCTT CGCTCTTGAG GTCCTCCACT 
AAAAAATCCA CCAGTCCGGA AACCAAGCAC AGTGAGTTTT TGTCCATTGC TGGTACCACC 
ACCTCGGATT ATTCTACCAC ATCATCCACT ACCTACTTGA CCAGCCTGGA CTCCAGCCGC 
CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG AGCAAGGGGG ATGAGGCTGA TGACGAGAGG 
AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG GAGACCGACA GTGAGAGTGA GTTCCCCGTG 
TTTCCCACCA CCCTGACTTC AGACCGGCTG TTCCGAGGAA AATTCCAGGA GGTTGCCAGG 
GTGAGCCGCA GGAACTCCGA AGGCAGTGAG GCCAGCTGCA CCGAGGGAAG CCTCACACCG 
AGTTTAGACA GCCGCAGGCA GCAGTTCAGC TCCCATAGAC TGATCGAATG CGACACACTG 
TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA GACAGTGGGA GCCCCGGAGA CACCAGGACT 
GAGAAAGAAA CTCCTGCCCT GGCCAAGATG TTTGACGTCA TGAAGAAAGG CAAGTCGACA 
GGGAGCCTCC TCACACCCAG CAGAAGTGAA TCCGAAAAGC AGGAGGCCAC TTGGAAAACA 
AAAATAGCAG ATCGTTTAAA ACTGAGACCC AGGGCACCCG CAGACGACAT GTTTGGAGTT 
GGGAATCAAA AACCTACTGC TGAGACAGCG AAAAGGAAAA ACATCAAACG CAGACACACG 
CTTGGAGGCC ACAGAGATGC TACTGAGATC AGCGTCCTGA GTTTCTGGAA AGCTCATGAG 
CAGAGTGCGG ACAAAGAGTC TGAACTTTCG GCTGTGAATC GGTTAAAACC CAAATGCTCA 
GCCCAGGACC TTTCCATCTC AGACTGGCTC GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC 
GACCTTAGCA GAGGCGAAGG CCTCGAGCCC CAGGCTGAGA GCCCCAGCGT GCTGGGAACA 
CCCATCAGCA CCCACTCACC ACCGTCTCAG CAGCCCGAAG CAAGGGTTGC TGCTACCAGC 
ACCTTGGCTT CAACCAGCCA GTCCCCTCTT TTCACACCAC CACAGTCACC TGACCAAATA 
AACAGAGAAA GCTTCCAGAA CATGAGCCAA AATGCTAGTT CTACAGCTAA CATCCACCCC 
CACAAACAGT CTGAAAGTCC AGACACTAAA GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121556.2
CDS	312..6179
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	NM_001128084.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128084.2

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AAAACAGAAC AGTTGCCAAA AACACCTCGC 
CAGAGTCTTA GCATCAGGCA AACTTTGCTT GGTGCTAAAT CAGAGCCAAA GACTCAAAGC 
CCACATTCTC CAAAGGAGGA GTCCGAGAGG AAGCTTCTCA GTAAAGATGA TACCAGTCCC 
CCTAAAGACA AAGGTACTTG GAGAAGAGGC ATTCCAAGCA TCGTGAGGAA GACGTTTGAG 
AAAAAGCCGG CCGCCACAGG GACCTTTGGA GTTAGGCTGG ATGACTGCCC ACCCGCCCAC 
ACCAACAGGT ACATTCCATT AATCGTTGAC ATATGTTGCA AATTAGTTGA AGAAAGGGGT 
CTTGAATATA CAGGTATTTA CCGAGTTCCC GGCAACAACG CAGCCATTTC AAGCATGCAG 
GAAGAACTCA ACAAGGGGAT GGCAGACATT GACATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATAA GCAGTTTACT AAAATCCTTC TTCAGAAAGC TCCCTGAGCC TCTCTTTACG 
AATGACAAAT ATGCTGACTT CATTGAGGCC AATCGCAAAG AGGACCCACT GGATCGGCTA 
CGAACATTAA AGAGACTAAT TCATGACCTG CCTGAGCATC ATTTTGAGAC GCTCAAGTTT 
CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG AACTCAGAAA AGAATAAGAT GGAACCCAGA 
AACCTAGCGA TCGTTTTTGG TCCAACTCTA GTGAGAACAT CAGAAGACAA CATGACCCAC 
ATGGTCACGC ACATGCCTGA CCAGTATAAG ATCGTAGAGA CTCTCATCCA GCATCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG CCTCTTACAG CAGTGCAGGA GGAGAACACA 
GTAGACTCCC AGCCAGTACC AAACATAGAT CATTTGCTCA CCAACATTGG AAGGACAGGC 
GTGCTCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CCGCAAAATC TAAGGGCTCC 
TGGGGATCCG GGAAGGATCA GTATAGCAGG GAACTGCTTG TATCCTCCAT CTTCGCAGCT 
GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG AAAGCCCAGC CCAGCAGCTC TGAGGATGAG 
CTGGACAGTG TGTTCTTTAA GAAGGAGAAT ACGGAACAGA GTCACAGTGA GATTAAAGAA 
GAGTCCAAAA GAGAAAGCGA GACATCAGGT AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA 
AGTAACACTA AGAAAGACTC GGGTACAACG AAGGAGGAGA AGAAGATACC GTGGGAAGAA 
CCATCCCCAC CACACAGCTC CAAACGCAAC AGGTCACCAA CCCTCAGCTG TCGCCTGGCC 
ATGCTGAAAG AGGGCCCCAG ATCACTTCTG ACACAGAAGC CCCACTGTGA AGAGACAGGG 
TCTGACTCAG GCACTCTGCT TAGCACGTCT TCCCAGGCTT CGCTCTTGAG GTCCTCCACT 
AAAAAATCCA CCAGTCCGGA AACCAAGCAC AGTGAGTTTT TGTCCATTGC TGGTACCACC 
ACCTCGGATT ATTCTACCAC ATCATCCACT ACCTACTTGA CCAGCCTGGA CTCCAGCCGC 
CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG AGCAAGGGGG ATGAGGCTGA TGACGAGAGG 
AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG GAGACCGACA GTGAGAGTGA GTTCCCCGTG 
TTTCCCACCA CCCTGACTTC AGACCGGCTG TTCCGAGGAA AATTCCAGGA GGTTGCCAGG 
GTGAGCCGCA GGAACTCCGA AGGCAGTGAG GCCAGCTGCA CCGAGGGAAG CCTCACACCG 
AGTTTAGACA GCCGCAGGCA GCAGTTCAGC TCCCATAGAC TGATCGAATG CGACACACTG 
TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA GACAGTGGGA GCCCCGGAGA CACCAGGACT 
GAGAAAGAAA CTCCTGCCCT GGCCAAGATG TTTGACGTCA TGAAGAAAGG CAAGTCGACA 
GGGAGCCTCC TCACACCCAG CAGAAGTGAA TCCGAAAAGC AGGAGGCCAC TTGGAAAACA 
AAAATAGCAG ATCGTTTAAA ACTGAGACCC AGGGCACCCG CAGACGACAT GTTTGGAGTT 
GGGAATCAAA AACCTACTGC TGAGACAGCG AAAAGGAAAA ACATCAAACG CAGACACACG 
CTTGGAGGCC ACAGAGATGC TACTGAGATC AGCGTCCTGA GTTTCTGGAA AGCTCATGAG 
CAGAGTGCGG ACAAAGAGTC TGAACTTTCG GCTGTGAATC GGTTAAAACC CAAATGCTCA 
GCCCAGGACC TTTCCATCTC AGACTGGCTC GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC 
GACCTTAGCA GAGGCGAAGG CCTCGAGCCC CAGGCTGAGA GCCCCAGCGT GCTGGGAACA 
CCCATCAGCA CCCACTCACC ACCGTCTCAG CAGCCCGAAG CAAGGGTTGC TGCTACCAGC 
ACCTTGGCTT CAACCAGCCA GTCCCCTCTT TTCACACCAC CACAGTCACC TGACCAAATA 
AACAGAGAAA GCTTCCAGAA CATGAGCCAA AATGCTAGTT CTACAGCTAA CATCCACCCC 
CACAAACAGT CTGAAAGTCC AGACACTAAA GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497545.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497608.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X8, mRNA.

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CloneID	OMu02220
Clone ID Related Accession (Same CDS sequence)	NM_001081364.3 , XM_006497545.3 , XM_006497545.4
Accession Version	XM_006497545.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497545.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AAAACAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTTA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACATTCTC CAAAGGAGGA GTCCGAGAGG 
AAGCTTCTCA GTAAAGATGA TACCAGTCCC CCTAAAGACA AAGGTACTTG GAGAAGAGGC 
ATTCCAAGCA TCGTGAGGAA GACGTTTGAG AAAAAGCCGG CCGCCACAGG GACCTTTGGA 
GTTAGGCTGG ATGACTGCCC ACCCGCCCAC ACCAACAGGT ACATTCCATT AATCGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGGGGT CTTGAATATA CAGGTATTTA CCGAGTTCCC 
GGCAACAACG CAGCCATTTC AAGCATGCAG GAAGAACTCA ACAAGGGGAT GGCAGACATT 
GACATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAGC TCCCTGAGCC TCTCTTTACG AATGACAAAT ATGCTGACTT CATTGAGGCC 
AATCGCAAAG AGGACCCACT GGATCGGCTA CGAACATTAA AGAGACTAAT TCATGACCTG 
CCTGAGCATC ATTTTGAGAC GCTCAAGTTT CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG 
AACTCAGAAA AGAATAAGAT GGAACCCAGA AACCTAGCGA TCGTTTTTGG TCCAACTCTA 
GTGAGAACAT CAGAAGACAA CATGACCCAC ATGGTCACGC ACATGCCTGA CCAGTATAAG 
ATCGTAGAGA CTCTCATCCA GCATCATGAC TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG 
CCTCTTACAG CAGTGCAGGA GGAGAACACA GTAGACTCCC AGCCAGTACC AAACATAGAT 
CATTTGCTCA CCAACATTGG AAGGACAGGC GTGCTCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CCGCAAAATC TAAGGGCTCC TGGGGATCCG GGAAGGATCA GTATAGCAGG 
GAACTGCTTG TATCCTCCAT CTTCGCAGCT GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG 
AAAGCCCAGC CCAGCAGCTC TGAGGATGAG CTGGACAGTG TGTTCTTTAA GAAGGAGAAT 
ACGGAACAGA GTCACAGTGA GATTAAAGAA GAGTCCAAAA GAGAAAGCGA GACATCAGGT 
AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA AGTAACACTA AGAAAGACTC GGGTACAACG 
AAGGAGGAGA AGAAGATACC GTGGGAAGAA CCATCCCCAC CACACAGCTC CAAACGCAAC 
AGGTCACCAA CCCTCAGCTG TCGCCTGGCC ATGCTGAAAG AGGGCCCCAG ATCACTTCTG 
ACACAGAAGC CCCACTGTGA AGAGACAGGG TCTGACTCAG GCACTCTGCT TAGCACGTCT 
TCCCAGGCTT CGCTCTTGAG GTCCTCCACT AAAAAATCCA CCAGTCCGGA AACCAAGCAC 
AGTGAGTTTT TGTCCATTGC TGGTACCACC ACCTCGGATT ATTCTACCAC ATCATCCACT 
ACCTACTTGA CCAGCCTGGA CTCCAGCCGC CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG 
AGCAAGGGGG ATGAGGCTGA TGACGAGAGG AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG 
GAGACCGACA GTGAGAGTGA GTTCCCCGTG TTTCCCACCA CCCTGACTTC AGACCGGCTG 
TTCCGAGGAA AATTCCAGGA GGTTGCCAGG GTGAGCCGCA GGAACTCCGA AGGCAGTGAG 
GCCAGCTGCA CCGAGGGAAG CCTCACACCG AGTTTAGACA GCCGCAGGCA GCAGTTCAGC 
TCCCATAGAC TGATCGAATG CGACACACTG TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA 
GACAGTGGGA GCCCCGGAGA CACCAGGACT GAGAAAGAAA CTCCTGCCCT GGCCAAGATG 
TTTGACGTCA TGAAGAAAGG CAAGTCGACA GGGAGCCTCC TCACACCCAG CAGAAGTGAA 
TCCGAAAAGC AGGAGGCCAC TTGGAAAACA AAAATAGCAG ATCGTTTAAA ACTGAGACCC 
AGGGCACCCG CAGACGACAT GTTTGGAGTT GGGAATCAAA AACCTACTGC TGAGACAGCG 
AAAAGGAAAA ACATCAAACG CAGACACACG CTTGGAGGCC ACAGAGATGC TACTGAGATC 
AGCGTCCTGA GTTTCTGGAA AGCTCATGAG CAGAGTGCGG ACAAAGAGTC TGAACTTTCG 
GCTGTGAATC GGTTAAAACC CAAATGCTCA GCCCAGGACC TTTCCATCTC AGACTGGCTC 
GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC GACCTTAGCA GAGGCGAAGG CCTCGAGCCC 
CAGGCTGAGA GCCCCAGCGT GCTGGGAACA CCCATCAGCA CCCACTCACC ACCGTCTCAG 
CAGCCCGAAG CAAGGGTTGC TGCTACCAGC ACCTTGGCTT CAACCAGCCA GTCCCCTCTT 
TTCACACCAC CACAGTCACC TGACCAAATA AACAGAGAAA GCTTCCAGAA CATGAGCCAA 
AATGCTAGTT CTACAGCTAA CATCCACCCC CACAAACAGT CTGAAAGTCC AGACACTAAA 
GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497608.1
CDS	472..6309
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497545.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497545.3

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGGCATATT CTCAAGATGC ATACCTGAAA 
GGCAATGAAG CTTACAGCGG CAATGCCCGT AATATACCTG AACCTCCACC AGTTTGCTAT 
CCCTGGTTGC CATCGACTCC TTCAGCTACT GCACAGCCGG TGGAAACATG CCCTCCTGAT 
TCATTACCGA ACAAACAGCA AACCAGTGCA CCAGTCCTCA CACAACCTGG CAGGGCCTAT 
AGAATGGAAA TACAAGTGCC TCCGTCACCA ACAGATGTTG CAAAGTCAAA CACAGCAGTG 
TGTGTTTGCA ATGAGAGTGT GAGGACCGTC ATTGTGCCTT CTGAGAAGGT GGTAGACTTG 
CTAGCCAATA GAAACAACCC TTCAGGTCCT TCACATAGAA CTGAAGAGGT AAGGTATGGT 
GTAAATGAGC AGGCCTCTAC AAAAGCAGCA TCGAGAACAA CGTCACCGGC ATCAGTTCCC 
ACTGCCCATC TGATTCATCA GACCACAGGC TCCAGATCCT TAGAACCTTC TGGAATTTTA 
CTTAAATCTG GAAATTACAG TGGACATTCA GAAGGAATCT CAAGCAGTAG ATCACAAGCC 
GTGGATTCCC CTCCTGTGTC TGTCAATCAC TACTCTGCAA ACTCCCATCA GCACATAGAC 
TGGAAAAATT ATAAGACTTA CAAAGAATAC ATTGATAATA GGAGATTGCA CATAGGTTGT 
CGGACAATCC AGGAAAGACT GGACAGCTTA CGAGCTGCAT CTCAGAGCGC AGCAGACTAT 
AACCAGGTGG TACCCACCCG CACTACTCTG CAGGTACGAC GTCGAAGCAC CTCTCATGAC 
CGAGTGCCCC AGTCTGTTCA GATCCGGCAG CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT 
TCTGTGCTGA TGAAGTATTG CCCAAGGAGC GCGTCTCAAG GAGCGCTGAC CTCACCGCCT 
GTTAGCTTCA ATAATCATAG AACTCGTTCG TGGGATTACA TAGAGGGACA GACGGAAGCC 
ACAGCCACTG TCAATTCTGA AAGTCAGATA CCTGACTCAA ATGGGGAACG AAAACAGACT 
TACAAGTGGA GTGGCTTCAC TGAGCAGGAT GATAGACGCG GTATTCACGA AAGACCCAGG 
CAGCAAGAAA TGCACAAGCC TTTCCGAGGC TCAAATTTGA CTGTGGCTCC CGTTGTTAAT 
TCTGATAACA GGCGCCTGGT TGGGAGAGGG GTAGGACCTG TCTCACAGTT TAAAAAAATC 
CCGCCGGACC TAAGACCACC ACATTCCAAC AGAAATTTTC CAACCACGAC TGGGGTGTCA 
CTTCAACGGG GTATTGCACA AGACAGGTCC CCACTCGTGA AAGTCCGAAG TAATTCTCTG 
AAAGTGCCAC CTCCACCTGT GTCAAAACCG TCCTTCAGCC AGCACTCACT GGCTTCCATG 
AAAGACCAAA GGCCTGTCAA TCATCTGCAT CAACACAGTG TGCTGAGTCA GCAGACGCAG 
TTCAGATCCG AAAGCACCTT CGAGCACCAG CTGGAGACTG AGGTATCCTC GTGTCTACCA 
GGAACTTCTG CCAAGACCAG TCCACAGCTC AGTGAGAATT TGGGAACATC AGACTTAGAA 
CTACCTGCCA TCCCAAGGAA TGGAGATATA AACTTACAGG AGGCTGAAAT TCAGCAGCCA 
GATGTGCTAG ATAATAAAGA ATCTGTCATT CTAAGAGAGA AACCTCAGTC TGGCCGCCAG 
ACGCCACAGC CTTTAAGACA TCAGTCTTAC ATCCTGGCAG TGAATGACCA GGAGACCGGG 
TCAGACACCA CCTGCTGGCT GCCAAACGAT GCACGCAGAG AGGTCCATAT AAAAAGAATG 
GAGGAGAGGA AAGCTTCAAG CACCAGCCCT CCTGGTGATT CCCTGGCATC CATCCCTTTT 
ATAGATGAGC CAACCAGTCC TAGCATCGAC CACGAGATTG CACATATTCC TGCATCTGCT 
GTCATCTCCG CCTCTACTGC CCACGTCCCC TCCATTGCAA CTGTTCCCCC CAGCCTCACG 
ACCTCTGCTC CTTTGATTCG ACGTCAGCTC TCCCACGACC AAGAATCTGT TGGACCTCCC 
AGCCTGGATG GCCAGCACAG CTCAAAGACA GAAAGATCCA AATCCTACGA TGAAGGACTG 
GATGATTATA GAGAAGATGC AAAACTATCC TTTAAGCACG TGTCTAGCCT GAAGGGAATC 
AAGATTACAG ACAGCCAGAA GTCATCAGAA GACTCTGGAT CGAGAAAGGG TTCTTCCTCC 
GAGGTCTTCA GTGATGCAGC CCGAGAAGGC TGGCTCCAGT TCCGACCACT GGTCACCGAT 
AAGGGCAAGA GAGTTGGTGG CAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGCCACTCAC TTTACTTGTA CAAAGACAGA AGAGAGCAGA CAACGCCATC AGAAGAAGAG 
CAGCCCATCA GTGTCAACGC TTGCCTGATA GACATCTCGT ACAGTGAGAC CAAGAGGCGG 
AATGTATTCC GACTTACCAC GTCTGACTGT GAATGCTTGT TCCAGGCTGA AGACAGAGAT 
GATATGTTAT CATGGATCAA GACTATTCAG GAAAGTAGCA ACCTAAATGA AGAGGACACT 
GGAGTTACCA ACAGAGATCT AATTAGTCGA AGAATCAAAG AGTATAACAG TCTGCTGAGC 
AAAACAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTTA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACATTCTC CAAAGGAGGA GTCCGAGAGG 
AAGCTTCTCA GTAAAGATGA TACCAGTCCC CCTAAAGACA AAGGTACTTG GAGAAGAGGC 
ATTCCAAGCA TCGTGAGGAA GACGTTTGAG AAAAAGCCGG CCGCCACAGG GACCTTTGGA 
GTTAGGCTGG ATGACTGCCC ACCCGCCCAC ACCAACAGGT ACATTCCATT AATCGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGGGGT CTTGAATATA CAGGTATTTA CCGAGTTCCC 
GGCAACAACG CAGCCATTTC AAGCATGCAG GAAGAACTCA ACAAGGGGAT GGCAGACATT 
GACATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAGC TCCCTGAGCC TCTCTTTACG AATGACAAAT ATGCTGACTT CATTGAGGCC 
AATCGCAAAG AGGACCCACT GGATCGGCTA CGAACATTAA AGAGACTAAT TCATGACCTG 
CCTGAGCATC ATTTTGAGAC GCTCAAGTTT CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG 
AACTCAGAAA AGAATAAGAT GGAACCCAGA AACCTAGCGA TCGTTTTTGG TCCAACTCTA 
GTGAGAACAT CAGAAGACAA CATGACCCAC ATGGTCACGC ACATGCCTGA CCAGTATAAG 
ATCGTAGAGA CTCTCATCCA GCATCATGAC TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG 
CCTCTTACAG CAGTGCAGGA GGAGAACACA GTAGACTCCC AGCCAGTACC AAACATAGAT 
CATTTGCTCA CCAACATTGG AAGGACAGGC GTGCTCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CCGCAAAATC TAAGGGCTCC TGGGGATCCG GGAAGGATCA GTATAGCAGG 
GAACTGCTTG TATCCTCCAT CTTCGCAGCT GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG 
AAAGCCCAGC CCAGCAGCTC TGAGGATGAG CTGGACAGTG TGTTCTTTAA GAAGGAGAAT 
ACGGAACAGA GTCACAGTGA GATTAAAGAA GAGTCCAAAA GAGAAAGCGA GACATCAGGT 
AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA AGTAACACTA AGAAAGACTC GGGTACAACG 
AAGGAGGAGA AGAAGATACC GTGGGAAGAA CCATCCCCAC CACACAGCTC CAAACGCAAC 
AGGTCACCAA CCCTCAGCTG TCGCCTGGCC ATGCTGAAAG AGGGCCCCAG ATCACTTCTG 
ACACAGAAGC CCCACTGTGA AGAGACAGGG TCTGACTCAG GCACTCTGCT TAGCACGTCT 
TCCCAGGCTT CGCTCTTGAG GTCCTCCACT AAAAAATCCA CCAGTCCGGA AACCAAGCAC 
AGTGAGTTTT TGTCCATTGC TGGTACCACC ACCTCGGATT ATTCTACCAC ATCATCCACT 
ACCTACTTGA CCAGCCTGGA CTCCAGCCGC CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG 
AGCAAGGGGG ATGAGGCTGA TGACGAGAGG AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG 
GAGACCGACA GTGAGAGTGA GTTCCCCGTG TTTCCCACCA CCCTGACTTC AGACCGGCTG 
TTCCGAGGAA AATTCCAGGA GGTTGCCAGG GTGAGCCGCA GGAACTCCGA AGGCAGTGAG 
GCCAGCTGCA CCGAGGGAAG CCTCACACCG AGTTTAGACA GCCGCAGGCA GCAGTTCAGC 
TCCCATAGAC TGATCGAATG CGACACACTG TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA 
GACAGTGGGA GCCCCGGAGA CACCAGGACT GAGAAAGAAA CTCCTGCCCT GGCCAAGATG 
TTTGACGTCA TGAAGAAAGG CAAGTCGACA GGGAGCCTCC TCACACCCAG CAGAAGTGAA 
TCCGAAAAGC AGGAGGCCAC TTGGAAAACA AAAATAGCAG ATCGTTTAAA ACTGAGACCC 
AGGGCACCCG CAGACGACAT GTTTGGAGTT GGGAATCAAA AACCTACTGC TGAGACAGCG 
AAAAGGAAAA ACATCAAACG CAGACACACG CTTGGAGGCC ACAGAGATGC TACTGAGATC 
AGCGTCCTGA GTTTCTGGAA AGCTCATGAG CAGAGTGCGG ACAAAGAGTC TGAACTTTCG 
GCTGTGAATC GGTTAAAACC CAAATGCTCA GCCCAGGACC TTTCCATCTC AGACTGGCTC 
GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC GACCTTAGCA GAGGCGAAGG CCTCGAGCCC 
CAGGCTGAGA GCCCCAGCGT GCTGGGAACA CCCATCAGCA CCCACTCACC ACCGTCTCAG 
CAGCCCGAAG CAAGGGTTGC TGCTACCAGC ACCTTGGCTT CAACCAGCCA GTCCCCTCTT 
TTCACACCAC CACAGTCACC TGACCAAATA AACAGAGAAA GCTTCCAGAA CATGAGCCAA 
AATGCTAGTT CTACAGCTAA CATCCACCCC CACAAACAGT CTGAAAGTCC AGACACTAAA 
GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497543.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497606.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X6, mRNA.

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CloneID	OMu21534
Clone ID Related Accession (Same CDS sequence)	XM_006497543.3 , NM_001128084.2 , XM_006497543.4
Accession Version	XM_006497543.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AAAACAGAAC AGTTGCCAAA AACACCTCGC 
CAGAGTCTTA GCATCAGGCA AACTTTGCTT GGTGCTAAAT CAGAGCCAAA GACTCAAAGC 
CCACATTCTC CAAAGGAGGA GTCCGAGAGG AAGCTTCTCA GTAAAGATGA TACCAGTCCC 
CCTAAAGACA AAGGTACTTG GAGAAGAGGC ATTCCAAGCA TCGTGAGGAA GACGTTTGAG 
AAAAAGCCGG CCGCCACAGG GACCTTTGGA GTTAGGCTGG ATGACTGCCC ACCCGCCCAC 
ACCAACAGGT ACATTCCATT AATCGTTGAC ATATGTTGCA AATTAGTTGA AGAAAGGGGT 
CTTGAATATA CAGGTATTTA CCGAGTTCCC GGCAACAACG CAGCCATTTC AAGCATGCAG 
GAAGAACTCA ACAAGGGGAT GGCAGACATT GACATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATAA GCAGTTTACT AAAATCCTTC TTCAGAAAGC TCCCTGAGCC TCTCTTTACG 
AATGACAAAT ATGCTGACTT CATTGAGGCC AATCGCAAAG AGGACCCACT GGATCGGCTA 
CGAACATTAA AGAGACTAAT TCATGACCTG CCTGAGCATC ATTTTGAGAC GCTCAAGTTT 
CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG AACTCAGAAA AGAATAAGAT GGAACCCAGA 
AACCTAGCGA TCGTTTTTGG TCCAACTCTA GTGAGAACAT CAGAAGACAA CATGACCCAC 
ATGGTCACGC ACATGCCTGA CCAGTATAAG ATCGTAGAGA CTCTCATCCA GCATCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG CCTCTTACAG CAGTGCAGGA GGAGAACACA 
GTAGACTCCC AGCCAGTACC AAACATAGAT CATTTGCTCA CCAACATTGG AAGGACAGGC 
GTGCTCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CCGCAAAATC TAAGGGCTCC 
TGGGGATCCG GGAAGGATCA GTATAGCAGG GAACTGCTTG TATCCTCCAT CTTCGCAGCT 
GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG AAAGCCCAGC CCAGCAGCTC TGAGGATGAG 
CTGGACAGTG TGTTCTTTAA GAAGGAGAAT ACGGAACAGA GTCACAGTGA GATTAAAGAA 
GAGTCCAAAA GAGAAAGCGA GACATCAGGT AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA 
AGTAACACTA AGAAAGACTC GGGTACAACG AAGGAGGAGA AGAAGATACC GTGGGAAGAA 
CCATCCCCAC CACACAGCTC CAAACGCAAC AGGTCACCAA CCCTCAGCTG TCGCCTGGCC 
ATGCTGAAAG AGGGCCCCAG ATCACTTCTG ACACAGAAGC CCCACTGTGA AGAGACAGGG 
TCTGACTCAG GCACTCTGCT TAGCACGTCT TCCCAGGCTT CGCTCTTGAG GTCCTCCACT 
AAAAAATCCA CCAGTCCGGA AACCAAGCAC AGTGAGTTTT TGTCCATTGC TGGTACCACC 
ACCTCGGATT ATTCTACCAC ATCATCCACT ACCTACTTGA CCAGCCTGGA CTCCAGCCGC 
CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG AGCAAGGGGG ATGAGGCTGA TGACGAGAGG 
AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG GAGACCGACA GTGAGAGTGA GTTCCCCGTG 
TTTCCCACCA CCCTGACTTC AGACCGGCTG TTCCGAGGAA AATTCCAGGA GGTTGCCAGG 
GTGAGCCGCA GGAACTCCGA AGGCAGTGAG GCCAGCTGCA CCGAGGGAAG CCTCACACCG 
AGTTTAGACA GCCGCAGGCA GCAGTTCAGC TCCCATAGAC TGATCGAATG CGACACACTG 
TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA GACAGTGGGA GCCCCGGAGA CACCAGGACT 
GAGAAAGAAA CTCCTGCCCT GGCCAAGATG TTTGACGTCA TGAAGAAAGG CAAGTCGACA 
GGGAGCCTCC TCACACCCAG CAGAAGTGAA TCCGAAAAGC AGGAGGCCAC TTGGAAAACA 
AAAATAGCAG ATCGTTTAAA ACTGAGACCC AGGGCACCCG CAGACGACAT GTTTGGAGTT 
GGGAATCAAA AACCTACTGC TGAGACAGCG AAAAGGAAAA ACATCAAACG CAGACACACG 
CTTGGAGGCC ACAGAGATGC TACTGAGATC AGCGTCCTGA GTTTCTGGAA AGCTCATGAG 
CAGAGTGCGG ACAAAGAGTC TGAACTTTCG GCTGTGAATC GGTTAAAACC CAAATGCTCA 
GCCCAGGACC TTTCCATCTC AGACTGGCTC GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC 
GACCTTAGCA GAGGCGAAGG CCTCGAGCCC CAGGCTGAGA GCCCCAGCGT GCTGGGAACA 
CCCATCAGCA CCCACTCACC ACCGTCTCAG CAGCCCGAAG CAAGGGTTGC TGCTACCAGC 
ACCTTGGCTT CAACCAGCCA GTCCCCTCTT TTCACACCAC CACAGTCACC TGACCAAATA 
AACAGAGAAA GCTTCCAGAA CATGAGCCAA AATGCTAGTT CTACAGCTAA CATCCACCCC 
CACAAACAGT CTGAAAGTCC AGACACTAAA GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497606.1
CDS	472..6339
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497543.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497543.3

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AAAACAGAAC AGTTGCCAAA AACACCTCGC 
CAGAGTCTTA GCATCAGGCA AACTTTGCTT GGTGCTAAAT CAGAGCCAAA GACTCAAAGC 
CCACATTCTC CAAAGGAGGA GTCCGAGAGG AAGCTTCTCA GTAAAGATGA TACCAGTCCC 
CCTAAAGACA AAGGTACTTG GAGAAGAGGC ATTCCAAGCA TCGTGAGGAA GACGTTTGAG 
AAAAAGCCGG CCGCCACAGG GACCTTTGGA GTTAGGCTGG ATGACTGCCC ACCCGCCCAC 
ACCAACAGGT ACATTCCATT AATCGTTGAC ATATGTTGCA AATTAGTTGA AGAAAGGGGT 
CTTGAATATA CAGGTATTTA CCGAGTTCCC GGCAACAACG CAGCCATTTC AAGCATGCAG 
GAAGAACTCA ACAAGGGGAT GGCAGACATT GACATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATAA GCAGTTTACT AAAATCCTTC TTCAGAAAGC TCCCTGAGCC TCTCTTTACG 
AATGACAAAT ATGCTGACTT CATTGAGGCC AATCGCAAAG AGGACCCACT GGATCGGCTA 
CGAACATTAA AGAGACTAAT TCATGACCTG CCTGAGCATC ATTTTGAGAC GCTCAAGTTT 
CTCTCGGCTC ATCTGAAGAC AGTGGCAGAG AACTCAGAAA AGAATAAGAT GGAACCCAGA 
AACCTAGCGA TCGTTTTTGG TCCAACTCTA GTGAGAACAT CAGAAGACAA CATGACCCAC 
ATGGTCACGC ACATGCCTGA CCAGTATAAG ATCGTAGAGA CTCTCATCCA GCATCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCCGAAGAG CCTCTTACAG CAGTGCAGGA GGAGAACACA 
GTAGACTCCC AGCCAGTACC AAACATAGAT CATTTGCTCA CCAACATTGG AAGGACAGGC 
GTGCTCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CCGCAAAATC TAAGGGCTCC 
TGGGGATCCG GGAAGGATCA GTATAGCAGG GAACTGCTTG TATCCTCCAT CTTCGCAGCT 
GCCAGTCGCA AGAGGAAAAA GCCGAAAGAG AAAGCCCAGC CCAGCAGCTC TGAGGATGAG 
CTGGACAGTG TGTTCTTTAA GAAGGAGAAT ACGGAACAGA GTCACAGTGA GATTAAAGAA 
GAGTCCAAAA GAGAAAGCGA GACATCAGGT AGCAAGCAGA GGGTCGTCGT TGCCAAAGAA 
AGTAACACTA AGAAAGACTC GGGTACAACG AAGGAGGAGA AGAAGATACC GTGGGAAGAA 
CCATCCCCAC CACACAGCTC CAAACGCAAC AGGTCACCAA CCCTCAGCTG TCGCCTGGCC 
ATGCTGAAAG AGGGCCCCAG ATCACTTCTG ACACAGAAGC CCCACTGTGA AGAGACAGGG 
TCTGACTCAG GCACTCTGCT TAGCACGTCT TCCCAGGCTT CGCTCTTGAG GTCCTCCACT 
AAAAAATCCA CCAGTCCGGA AACCAAGCAC AGTGAGTTTT TGTCCATTGC TGGTACCACC 
ACCTCGGATT ATTCTACCAC ATCATCCACT ACCTACTTGA CCAGCCTGGA CTCCAGCCGC 
CTGAGCCCTG AGGTGCAGTC AGTGGCCGAG AGCAAGGGGG ATGAGGCTGA TGACGAGAGG 
AGCGAGCTGG TGAGTGAAGG ACGGCCAGTG GAGACCGACA GTGAGAGTGA GTTCCCCGTG 
TTTCCCACCA CCCTGACTTC AGACCGGCTG TTCCGAGGAA AATTCCAGGA GGTTGCCAGG 
GTGAGCCGCA GGAACTCCGA AGGCAGTGAG GCCAGCTGCA CCGAGGGAAG CCTCACACCG 
AGTTTAGACA GCCGCAGGCA GCAGTTCAGC TCCCATAGAC TGATCGAATG CGACACACTG 
TCCAGGAAGA AGTCTGCTCG CTTCAAGTCA GACAGTGGGA GCCCCGGAGA CACCAGGACT 
GAGAAAGAAA CTCCTGCCCT GGCCAAGATG TTTGACGTCA TGAAGAAAGG CAAGTCGACA 
GGGAGCCTCC TCACACCCAG CAGAAGTGAA TCCGAAAAGC AGGAGGCCAC TTGGAAAACA 
AAAATAGCAG ATCGTTTAAA ACTGAGACCC AGGGCACCCG CAGACGACAT GTTTGGAGTT 
GGGAATCAAA AACCTACTGC TGAGACAGCG AAAAGGAAAA ACATCAAACG CAGACACACG 
CTTGGAGGCC ACAGAGATGC TACTGAGATC AGCGTCCTGA GTTTCTGGAA AGCTCATGAG 
CAGAGTGCGG ACAAAGAGTC TGAACTTTCG GCTGTGAATC GGTTAAAACC CAAATGCTCA 
GCCCAGGACC TTTCCATCTC AGACTGGCTC GCCAGGGAGC GCGTCCGCAC CAGTGCCTCC 
GACCTTAGCA GAGGCGAAGG CCTCGAGCCC CAGGCTGAGA GCCCCAGCGT GCTGGGAACA 
CCCATCAGCA CCCACTCACC ACCGTCTCAG CAGCCCGAAG CAAGGGTTGC TGCTACCAGC 
ACCTTGGCTT CAACCAGCCA GTCCCCTCTT TTCACACCAC CACAGTCACC TGACCAAATA 
AACAGAGAAA GCTTCCAGAA CATGAGCCAA AATGCTAGTT CTACAGCTAA CATCCACCCC 
CACAAACAGT CTGAAAGTCC AGACACTAAA GCAGAGACTC CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497538.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497601.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X1, mRNA.

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CloneID	OMu29195
Clone ID Related Accession (Same CDS sequence)	XM_006497538.3 , XM_006497538.4
Accession Version	XM_006497538.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAATTCAT GACCTGCCTG AGCATCATTT TGAGACGCTC 
AAGTTTCTCT CGGCTCATCT GAAGACAGTG GCAGAGAACT CAGAAAAGAA TAAGATGGAA 
CCCAGAAACC TAGCGATCGT TTTTGGTCCA ACTCTAGTGA GAACATCAGA AGACAACATG 
ACCCACATGG TCACGCACAT GCCTGACCAG TATAAGATCG TAGAGACTCT CATCCAGCAT 
CATGACTGGT TTTTCACAGA AGAAGGTGCC GAAGAGCCTC TTACAGCAGT GCAGGAGGAG 
AACACAGTAG ACTCCCAGCC AGTACCAAAC ATAGATCATT TGCTCACCAA CATTGGAAGG 
ACAGGCGTGC TCCCTGGAGA TGTATCAGAT TCAGCTACTA GTGACTCCGC AAAATCTAAG 
GGCTCCTGGG GATCCGGGAA GGATCAGTAT AGCAGGGAAC TGCTTGTATC CTCCATCTTC 
GCAGCTGCCA GTCGCAAGAG GAAAAAGCCG AAAGAGAAAG CCCAGCCCAG CAGCTCTGAG 
GATGAGCTGG ACAGTGTGTT CTTTAAGAAG GAGAATACGG AACAGAGTCA CAGTGAGATT 
AAAGAAGAGT CCAAAAGAGA AAGCGAGACA TCAGGTAGCA AGCAGAGGGT CGTCGTTGCC 
AAAGAAAGTA ACACTAAGAA AGACTCGGGT ACAACGAAGG AGGAGAAGAA GATACCGTGG 
GAAGAACCAT CCCCACCACA CAGCTCCAAA CGCAACAGGT CACCAACCCT CAGCTGTCGC 
CTGGCCATGC TGAAAGAGGG CCCCAGATCA CTTCTGACAC AGAAGCCCCA CTGTGAAGAG 
ACAGGGTCTG ACTCAGGCAC TCTGCTTAGC ACGTCTTCCC AGGCTTCGCT CTTGAGGTCC 
TCCACTAAAA AATCCACCAG TCCGGAAACC AAGCACAGTG AGTTTTTGTC CATTGCTGGT 
ACCACCACCT CGGATTATTC TACCACATCA TCCACTACCT ACTTGACCAG CCTGGACTCC 
AGCCGCCTGA GCCCTGAGGT GCAGTCAGTG GCCGAGAGCA AGGGGGATGA GGCTGATGAC 
GAGAGGAGCG AGCTGGTGAG TGAAGGACGG CCAGTGGAGA CCGACAGTGA GAGTGAGTTC 
CCCGTGTTTC CCACCACCCT GACTTCAGAC CGGCTGTTCC GAGGAAAATT CCAGGAGGTT 
GCCAGGGTGA GCCGCAGGAA CTCCGAAGGC AGTGAGGCCA GCTGCACCGA GGGAAGCCTC 
ACACCGAGTT TAGACAGCCG CAGGCAGCAG TTCAGCTCCC ATAGACTGAT CGAATGCGAC 
ACACTGTCCA GGAAGAAGTC TGCTCGCTTC AAGTCAGACA GTGGGAGCCC CGGAGACACC 
AGGACTGAGA AAGAAACTCC TGCCCTGGCC AAGATGTTTG ACGTCATGAA GAAAGGCAAG 
TCGACAGGGA GCCTCCTCAC ACCCAGCAGA AGTGAATCCG AAAAGCAGGA GGCCACTTGG 
AAAACAAAAA TAGCAGATCG TTTAAAACTG AGACCCAGGG CACCCGCAGA CGACATGTTT 
GGAGTTGGGA ATCAAAAACC TACTGCTGAG ACAGCGAAAA GGAAAAACAT CAAACGCAGA 
CACACGCTTG GAGGCCACAG AGATGCTACT GAGATCAGCG TCCTGAGTTT CTGGAAAGCT 
CATGAGCAGA GTGCGGACAA AGAGTCTGAA CTTTCGGCTG TGAATCGGTT AAAACCCAAA 
TGCTCAGCCC AGGACCTTTC CATCTCAGAC TGGCTCGCCA GGGAGCGCGT CCGCACCAGT 
GCCTCCGACC TTAGCAGAGG CGAAGGCCTC GAGCCCCAGG CTGAGAGCCC CAGCGTGCTG 
GGAACACCCA TCAGCACCCA CTCACCACCG TCTCAGCAGC CCGAAGCAAG GGTTGCTGCT 
ACCAGCACCT TGGCTTCAAC CAGCCAGTCC CCTCTTTTCA CACCACCACA GTCACCTGAC 
CAAATAAACA GAGAAAGCTT CCAGAACATG AGCCAAAATG CTAGTTCTAC AGCTAACATC 
CACCCCCACA AACAGTCTGA AAGTCCAGAC ACTAAAGCAG AGACTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497601.1
CDS	1897..7770
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497538.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497538.3

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAATTCAT GACCTGCCTG AGCATCATTT TGAGACGCTC 
AAGTTTCTCT CGGCTCATCT GAAGACAGTG GCAGAGAACT CAGAAAAGAA TAAGATGGAA 
CCCAGAAACC TAGCGATCGT TTTTGGTCCA ACTCTAGTGA GAACATCAGA AGACAACATG 
ACCCACATGG TCACGCACAT GCCTGACCAG TATAAGATCG TAGAGACTCT CATCCAGCAT 
CATGACTGGT TTTTCACAGA AGAAGGTGCC GAAGAGCCTC TTACAGCAGT GCAGGAGGAG 
AACACAGTAG ACTCCCAGCC AGTACCAAAC ATAGATCATT TGCTCACCAA CATTGGAAGG 
ACAGGCGTGC TCCCTGGAGA TGTATCAGAT TCAGCTACTA GTGACTCCGC AAAATCTAAG 
GGCTCCTGGG GATCCGGGAA GGATCAGTAT AGCAGGGAAC TGCTTGTATC CTCCATCTTC 
GCAGCTGCCA GTCGCAAGAG GAAAAAGCCG AAAGAGAAAG CCCAGCCCAG CAGCTCTGAG 
GATGAGCTGG ACAGTGTGTT CTTTAAGAAG GAGAATACGG AACAGAGTCA CAGTGAGATT 
AAAGAAGAGT CCAAAAGAGA AAGCGAGACA TCAGGTAGCA AGCAGAGGGT CGTCGTTGCC 
AAAGAAAGTA ACACTAAGAA AGACTCGGGT ACAACGAAGG AGGAGAAGAA GATACCGTGG 
GAAGAACCAT CCCCACCACA CAGCTCCAAA CGCAACAGGT CACCAACCCT CAGCTGTCGC 
CTGGCCATGC TGAAAGAGGG CCCCAGATCA CTTCTGACAC AGAAGCCCCA CTGTGAAGAG 
ACAGGGTCTG ACTCAGGCAC TCTGCTTAGC ACGTCTTCCC AGGCTTCGCT CTTGAGGTCC 
TCCACTAAAA AATCCACCAG TCCGGAAACC AAGCACAGTG AGTTTTTGTC CATTGCTGGT 
ACCACCACCT CGGATTATTC TACCACATCA TCCACTACCT ACTTGACCAG CCTGGACTCC 
AGCCGCCTGA GCCCTGAGGT GCAGTCAGTG GCCGAGAGCA AGGGGGATGA GGCTGATGAC 
GAGAGGAGCG AGCTGGTGAG TGAAGGACGG CCAGTGGAGA CCGACAGTGA GAGTGAGTTC 
CCCGTGTTTC CCACCACCCT GACTTCAGAC CGGCTGTTCC GAGGAAAATT CCAGGAGGTT 
GCCAGGGTGA GCCGCAGGAA CTCCGAAGGC AGTGAGGCCA GCTGCACCGA GGGAAGCCTC 
ACACCGAGTT TAGACAGCCG CAGGCAGCAG TTCAGCTCCC ATAGACTGAT CGAATGCGAC 
ACACTGTCCA GGAAGAAGTC TGCTCGCTTC AAGTCAGACA GTGGGAGCCC CGGAGACACC 
AGGACTGAGA AAGAAACTCC TGCCCTGGCC AAGATGTTTG ACGTCATGAA GAAAGGCAAG 
TCGACAGGGA GCCTCCTCAC ACCCAGCAGA AGTGAATCCG AAAAGCAGGA GGCCACTTGG 
AAAACAAAAA TAGCAGATCG TTTAAAACTG AGACCCAGGG CACCCGCAGA CGACATGTTT 
GGAGTTGGGA ATCAAAAACC TACTGCTGAG ACAGCGAAAA GGAAAAACAT CAAACGCAGA 
CACACGCTTG GAGGCCACAG AGATGCTACT GAGATCAGCG TCCTGAGTTT CTGGAAAGCT 
CATGAGCAGA GTGCGGACAA AGAGTCTGAA CTTTCGGCTG TGAATCGGTT AAAACCCAAA 
TGCTCAGCCC AGGACCTTTC CATCTCAGAC TGGCTCGCCA GGGAGCGCGT CCGCACCAGT 
GCCTCCGACC TTAGCAGAGG CGAAGGCCTC GAGCCCCAGG CTGAGAGCCC CAGCGTGCTG 
GGAACACCCA TCAGCACCCA CTCACCACCG TCTCAGCAGC CCGAAGCAAG GGTTGCTGCT 
ACCAGCACCT TGGCTTCAAC CAGCCAGTCC CCTCTTTTCA CACCACCACA GTCACCTGAC 
CAAATAAACA GAGAAAGCTT CCAGAACATG AGCCAAAATG CTAGTTCTAC AGCTAACATC 
CACCCCCACA AACAGTCTGA AAGTCCAGAC ACTAAAGCAG AGACTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497541.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497604.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X5, mRNA.

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CloneID	OMu29196
Clone ID Related Accession (Same CDS sequence)	XM_006497541.3 , XM_006497540.3 , XM_006497539.3 , XM_006497539.4 , XM_006497540.4 , XM_006497541.4
Accession Version	XM_006497541.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497541.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497604.1
CDS	507..6377
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497541.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497541.3

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497540.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497603.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X3, mRNA.

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CloneID	OMu29196
Clone ID Related Accession (Same CDS sequence)	XM_006497541.3 , XM_006497540.3 , XM_006497539.3 , XM_006497539.4 , XM_006497540.4 , XM_006497541.4
Accession Version	XM_006497540.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497540.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497603.1
CDS	468..6338
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497540.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497540.3

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497539.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497602.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X2, mRNA.

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CloneID	OMu29196
Clone ID Related Accession (Same CDS sequence)	XM_006497541.3 , XM_006497540.3 , XM_006497539.3 , XM_006497539.4 , XM_006497540.4 , XM_006497541.4
Accession Version	XM_006497539.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497539.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497602.1
CDS	472..6342
Translation	MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497539.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497539.3

ORF Insert Sequence:

ATGATGGCCA CACATTGGAC TGGTCTGCCT GAGGAAGATG GTGACAAACT CAAGGCCTGT 
GGGGCGGCCT CTGCCTGTGA GGTGTCAAAA AACAAAGATG GAAAAGACCA AGGTGAACCT 
GTGTCACCGT CTGAAGATGA GCCATTCTCC TGGCCAGGTC CCAAAACAGT CATGCTGAAG 
AGAACATCTC AAGGGTTTGG TTTCACATTA CGACACTTCA TCGTTTATCC CCCAGAGTCT 
GCAATCCAGT TTTCATATAA GGATGAAGAG AATGGAAACA GAGGAGGAAA GCAAAGGAAC 
CGATTGGAGC CGATGGATAC CATATTTGTT AAACAAGTGA AGGAAGGAGG GCCGGCCTTC 
GAGGCTGGCT TATGCACAGG TGACAGAATT ATAAAAGTCA ATGGAGAAAG TGTCATAGGA 
AAGACCTATT CCCAAGTAAT TGCTCTCATT CAGAACAGTG ACACCACCCT GGAGCTCAGT 
GTAATGCCCA AGGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTTACAGCA 
CTGGCATATT CTCAAGATGC ATACCTGAAA GGCAATGAAG CTTACAGCGG CAATGCCCGT 
AATATACCTG AACCTCCACC AGTTTGCTAT CCCTGGTTGC CATCGACTCC TTCAGCTACT 
GCACAGCCGG TGGAAACATG CCCTCCTGAT TCATTACCGA ACAAACAGCA AACCAGTGCA 
CCAGTCCTCA CACAACCTGG CAGGGCCTAT AGAATGGAAA TACAAGTGCC TCCGTCACCA 
ACAGATGTTG CAAAGTCAAA CACAGCAGTG TGTGTTTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTGCCTT CTGAGAAGGT GGTAGACTTG CTAGCCAATA GAAACAACCC TTCAGGTCCT 
TCACATAGAA CTGAAGAGGT AAGGTATGGT GTAAATGAGC AGGCCTCTAC AAAAGCAGCA 
TCGAGAACAA CGTCACCGGC ATCAGTTCCC ACTGCCCATC TGATTCATCA GACCACAGGC 
TCCAGATCCT TAGAACCTTC TGGAATTTTA CTTAAATCTG GAAATTACAG TGGACATTCA 
GAAGGAATCT CAAGCAGTAG ATCACAAGCC GTGGATTCCC CTCCTGTGTC TGTCAATCAC 
TACTCTGCAA ACTCCCATCA GCACATAGAC TGGAAAAATT ATAAGACTTA CAAAGAATAC 
ATTGATAATA GGAGATTGCA CATAGGTTGT CGGACAATCC AGGAAAGACT GGACAGCTTA 
CGAGCTGCAT CTCAGAGCGC AGCAGACTAT AACCAGGTGG TACCCACCCG CACTACTCTG 
CAGGTACGAC GTCGAAGCAC CTCTCATGAC CGAGTGCCCC AGTCTGTTCA GATCCGGCAG 
CGCAGTGTAT CTCAGGAAAG ACTGGAAGAT TCTGTGCTGA TGAAGTATTG CCCAAGGAGC 
GCGTCTCAAG GAGCGCTGAC CTCACCGCCT GTTAGCTTCA ATAATCATAG AACTCGTTCG 
TGGGATTACA TAGAGGGACA GACGGAAGCC ACAGCCACTG TCAATTCTGA AAGTCAGATA 
CCTGACTCAA ATGGGGAACG AAAACAGACT TACAAGTGGA GTGGCTTCAC TGAGCAGGAT 
GATAGACGCG GTATTCACGA AAGACCCAGG CAGCAAGAAA TGCACAAGCC TTTCCGAGGC 
TCAAATTTGA CTGTGGCTCC CGTTGTTAAT TCTGATAACA GGCGCCTGGT TGGGAGAGGG 
GTAGGACCTG TCTCACAGTT TAAAAAAATC CCGCCGGACC TAAGACCACC ACATTCCAAC 
AGAAATTTTC CAACCACGAC TGGGGTGTCA CTTCAACGGG GTATTGCACA AGACAGGTCC 
CCACTCGTGA AAGTCCGAAG TAATTCTCTG AAAGTGCCAC CTCCACCTGT GTCAAAACCG 
TCCTTCAGCC AGCACTCACT GGCTTCCATG AAAGACCAAA GGCCTGTCAA TCATCTGCAT 
CAACACAGTG TGCTGAGTCA GCAGACGCAG TTCAGATCCG AAAGCACCTT CGAGCACCAG 
CTGGAGACTG AGGTATCCTC GTGTCTACCA GGAACTTCTG CCAAGACCAG TCCACAGCTC 
AGTGAGAATT TGGGAACATC AGACTTAGAA CTACCTGCCA TCCCAAGGAA TGGAGATATA 
AACTTACAGG AGGCTGAAAT TCAGCAGCCA GATGTGCTAG ATAATAAAGA ATCTGTCATT 
CTAAGAGAGA AACCTCAGTC TGGCCGCCAG ACGCCACAGC CTTTAAGACA TCAGTCTTAC 
ATCCTGGCAG TGAATGACCA GGAGACCGGG TCAGACACCA CCTGCTGGCT GCCAAACGAT 
GCACGCAGAG AGGTCCATAT AAAAAGAATG GAGGAGAGGA AAGCTTCAAG CACCAGCCCT 
CCTGGTGATT CCCTGGCATC CATCCCTTTT ATAGATGAGC CAACCAGTCC TAGCATCGAC 
CACGAGATTG CACATATTCC TGCATCTGCT GTCATCTCCG CCTCTACTGC CCACGTCCCC 
TCCATTGCAA CTGTTCCCCC CAGCCTCACG ACCTCTGCTC CTTTGATTCG ACGTCAGCTC 
TCCCACGACC AAGAATCTGT TGGACCTCCC AGCCTGGATG GCCAGCACAG CTCAAAGACA 
GAAAGATCCA AATCCTACGA TGAAGGACTG GATGATTATA GAGAAGATGC AAAACTATCC 
TTTAAGCACG TGTCTAGCCT GAAGGGAATC AAGATTACAG ACAGCCAGAA GTCATCAGAA 
GACTCTGGAT CGAGAAAGGG TTCTTCCTCC GAGGTCTTCA GTGATGCAGC CCGAGAAGGC 
TGGCTCCAGT TCCGACCACT GGTCACCGAT AAGGGCAAGA GAGTTGGTGG CAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGCCACTCAC TTTACTTGTA CAAAGACAGA 
AGAGAGCAGA CAACGCCATC AGAAGAAGAG CAGCCCATCA GTGTCAACGC TTGCCTGATA 
GACATCTCGT ACAGTGAGAC CAAGAGGCGG AATGTATTCC GACTTACCAC GTCTGACTGT 
GAATGCTTGT TCCAGGCTGA AGACAGAGAT GATATGTTAT CATGGATCAA GACTATTCAG 
GAAAGTAGCA ACCTAAATGA AGAGGACACT GGAGTTACCA ACAGAGATCT AATTAGTCGA 
AGAATCAAAG AGTATAACAG TCTGCTGAGC AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497542.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497605.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X4, mRNA.

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CloneID	OMu29197
Clone ID Related Accession (Same CDS sequence)	XM_006497542.3 , XM_006497542.4
Accession Version	XM_006497542.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497542.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497605.1
CDS	1897..7767
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497542.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497542.3

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAAAACAG AACAGTTGCC AAAAACACCT 
CGCCAGAGTC TTAGCATCAG GCAAACTTTG CTTGGTGCTA AATCAGAGCC AAAGACTCAA 
AGCCCACATT CTCCAAAGGA GGAGTCCGAG AGGAAGCTTC TCAGTAAAGA TGATACCAGT 
CCCCCTAAAG ACAAAGGTAC TTGGAGAAGA GGCATTCCAA GCATCGTGAG GAAGACGTTT 
GAGAAAAAGC CGGCCGCCAC AGGGACCTTT GGAGTTAGGC TGGATGACTG CCCACCCGCC 
CACACCAACA GGTACATTCC ATTAATCGTT GACATATGTT GCAAATTAGT TGAAGAAAGG 
GGTCTTGAAT ATACAGGTAT TTACCGAGTT CCCGGCAACA ACGCAGCCAT TTCAAGCATG 
CAGGAAGAAC TCAACAAGGG GATGGCAGAC ATTGACATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TAAGCAGTTT ACTAAAATCC TTCTTCAGAA AGCTCCCTGA GCCTCTCTTT 
ACGAATGACA AATATGCTGA CTTCATTGAG GCCAATCGCA AAGAGGACCC ACTGGATCGG 
CTACGAACAT TAAAGAGACT AATTCATGAC CTGCCTGAGC ATCATTTTGA GACGCTCAAG 
TTTCTCTCGG CTCATCTGAA GACAGTGGCA GAGAACTCAG AAAAGAATAA GATGGAACCC 
AGAAACCTAG CGATCGTTTT TGGTCCAACT CTAGTGAGAA CATCAGAAGA CAACATGACC 
CACATGGTCA CGCACATGCC TGACCAGTAT AAGATCGTAG AGACTCTCAT CCAGCATCAT 
GACTGGTTTT TCACAGAAGA AGGTGCCGAA GAGCCTCTTA CAGCAGTGCA GGAGGAGAAC 
ACAGTAGACT CCCAGCCAGT ACCAAACATA GATCATTTGC TCACCAACAT TGGAAGGACA 
GGCGTGCTCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCCGCAAA ATCTAAGGGC 
TCCTGGGGAT CCGGGAAGGA TCAGTATAGC AGGGAACTGC TTGTATCCTC CATCTTCGCA 
GCTGCCAGTC GCAAGAGGAA AAAGCCGAAA GAGAAAGCCC AGCCCAGCAG CTCTGAGGAT 
GAGCTGGACA GTGTGTTCTT TAAGAAGGAG AATACGGAAC AGAGTCACAG TGAGATTAAA 
GAAGAGTCCA AAAGAGAAAG CGAGACATCA GGTAGCAAGC AGAGGGTCGT CGTTGCCAAA 
GAAAGTAACA CTAAGAAAGA CTCGGGTACA ACGAAGGAGG AGAAGAAGAT ACCGTGGGAA 
GAACCATCCC CACCACACAG CTCCAAACGC AACAGGTCAC CAACCCTCAG CTGTCGCCTG 
GCCATGCTGA AAGAGGGCCC CAGATCACTT CTGACACAGA AGCCCCACTG TGAAGAGACA 
GGGTCTGACT CAGGCACTCT GCTTAGCACG TCTTCCCAGG CTTCGCTCTT GAGGTCCTCC 
ACTAAAAAAT CCACCAGTCC GGAAACCAAG CACAGTGAGT TTTTGTCCAT TGCTGGTACC 
ACCACCTCGG ATTATTCTAC CACATCATCC ACTACCTACT TGACCAGCCT GGACTCCAGC 
CGCCTGAGCC CTGAGGTGCA GTCAGTGGCC GAGAGCAAGG GGGATGAGGC TGATGACGAG 
AGGAGCGAGC TGGTGAGTGA AGGACGGCCA GTGGAGACCG ACAGTGAGAG TGAGTTCCCC 
GTGTTTCCCA CCACCCTGAC TTCAGACCGG CTGTTCCGAG GAAAATTCCA GGAGGTTGCC 
AGGGTGAGCC GCAGGAACTC CGAAGGCAGT GAGGCCAGCT GCACCGAGGG AAGCCTCACA 
CCGAGTTTAG ACAGCCGCAG GCAGCAGTTC AGCTCCCATA GACTGATCGA ATGCGACACA 
CTGTCCAGGA AGAAGTCTGC TCGCTTCAAG TCAGACAGTG GGAGCCCCGG AGACACCAGG 
ACTGAGAAAG AAACTCCTGC CCTGGCCAAG ATGTTTGACG TCATGAAGAA AGGCAAGTCG 
ACAGGGAGCC TCCTCACACC CAGCAGAAGT GAATCCGAAA AGCAGGAGGC CACTTGGAAA 
ACAAAAATAG CAGATCGTTT AAAACTGAGA CCCAGGGCAC CCGCAGACGA CATGTTTGGA 
GTTGGGAATC AAAAACCTAC TGCTGAGACA GCGAAAAGGA AAAACATCAA ACGCAGACAC 
ACGCTTGGAG GCCACAGAGA TGCTACTGAG ATCAGCGTCC TGAGTTTCTG GAAAGCTCAT 
GAGCAGAGTG CGGACAAAGA GTCTGAACTT TCGGCTGTGA ATCGGTTAAA ACCCAAATGC 
TCAGCCCAGG ACCTTTCCAT CTCAGACTGG CTCGCCAGGG AGCGCGTCCG CACCAGTGCC 
TCCGACCTTA GCAGAGGCGA AGGCCTCGAG CCCCAGGCTG AGAGCCCCAG CGTGCTGGGA 
ACACCCATCA GCACCCACTC ACCACCGTCT CAGCAGCCCG AAGCAAGGGT TGCTGCTACC 
AGCACCTTGG CTTCAACCAG CCAGTCCCCT CTTTTCACAC CACCACAGTC ACCTGACCAA 
ATAAACAGAG AAAGCTTCCA GAACATGAGC CAAAATGCTA GTTCTACAGC TAACATCCAC 
CCCCACAAAC AGTCTGAAAG TCCAGACACT AAAGCAGAGA CTCCTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_006497544.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497607.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X7, mRNA.

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CloneID	OMu29198
Clone ID Related Accession (Same CDS sequence)	XM_006497544.4 , XM_006497544.3
Accession Version	XM_006497544.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497544.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGGCAT ATTCTCAAGA TGCATACCTG 
AAAGGCAATG AAGCTTACAG CGGCAATGCC CGTAATATAC CTGAACCTCC ACCAGTTTGC 
TATCCCTGGT TGCCATCGAC TCCTTCAGCT ACTGCACAGC CGGTGGAAAC ATGCCCTCCT 
GATTCATTAC CGAACAAACA GCAAACCAGT GCACCAGTCC TCACACAACC TGGCAGGGCC 
TATAGAATGG AAATACAAGT GCCTCCGTCA CCAACAGATG TTGCAAAGTC AAACACAGCA 
GTGTGTGTTT GCAATGAGAG TGTGAGGACC GTCATTGTGC CTTCTGAGAA GGTGGTAGAC 
TTGCTAGCCA ATAGAAACAA CCCTTCAGGT CCTTCACATA GAACTGAAGA GGTAAGGTAT 
GGTGTAAATG AGCAGGCCTC TACAAAAGCA GCATCGAGAA CAACGTCACC GGCATCAGTT 
CCCACTGCCC ATCTGATTCA TCAGACCACA GGCTCCAGAT CCTTAGAACC TTCTGGAATT 
TTACTTAAAT CTGGAAATTA CAGTGGACAT TCAGAAGGAA TCTCAAGCAG TAGATCACAA 
GCCGTGGATT CCCCTCCTGT GTCTGTCAAT CACTACTCTG CAAACTCCCA TCAGCACATA 
GACTGGAAAA ATTATAAGAC TTACAAAGAA TACATTGATA ATAGGAGATT GCACATAGGT 
TGTCGGACAA TCCAGGAAAG ACTGGACAGC TTACGAGCTG CATCTCAGAG CGCAGCAGAC 
TATAACCAGG TGGTACCCAC CCGCACTACT CTGCAGGTAC GACGTCGAAG CACCTCTCAT 
GACCGAGTGC CCCAGTCTGT TCAGATCCGG CAGCGCAGTG TATCTCAGGA AAGACTGGAA 
GATTCTGTGC TGATGAAGTA TTGCCCAAGG AGCGCGTCTC AAGGAGCGCT GACCTCACCG 
CCTGTTAGCT TCAATAATCA TAGAACTCGT TCGTGGGATT ACATAGAGGG ACAGACGGAA 
GCCACAGCCA CTGTCAATTC TGAAAGTCAG ATACCTGACT CAAATGGGGA ACGAAAACAG 
ACTTACAAGT GGAGTGGCTT CACTGAGCAG GATGATAGAC GCGGTATTCA CGAAAGACCC 
AGGCAGCAAG AAATGCACAA GCCTTTCCGA GGCTCAAATT TGACTGTGGC TCCCGTTGTT 
AATTCTGATA ACAGGCGCCT GGTTGGGAGA GGGGTAGGAC CTGTCTCACA GTTTAAAAAA 
ATCCCGCCGG ACCTAAGACC ACCACATTCC AACAGAAATT TTCCAACCAC GACTGGGGTG 
TCACTTCAAC GGGGTATTGC ACAAGACAGG TCCCCACTCG TGAAAGTCCG AAGTAATTCT 
CTGAAAGTGC CACCTCCACC TGTGTCAAAA CCGTCCTTCA GCCAGCACTC ACTGGCTTCC 
ATGAAAGACC AAAGGCCTGT CAATCATCTG CATCAACACA GTGTGCTGAG TCAGCAGACG 
CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC CAGCTGGAGA CTGAGGTATC CTCGTGTCTA 
CCAGGAACTT CTGCCAAGAC CAGTCCACAG CTCAGTGAGA ATTTGGGAAC ATCAGACTTA 
GAACTACCTG CCATCCCAAG GAATGGAGAT ATAAACTTAC AGGAGGCTGA AATTCAGCAG 
CCAGATGTGC TAGATAATAA AGAATCTGTC ATTCTAAGAG AGAAACCTCA GTCTGGCCGC 
CAGACGCCAC AGCCTTTAAG ACATCAGTCT TACATCCTGG CAGTGAATGA CCAGGAGACC 
GGGTCAGACA CCACCTGCTG GCTGCCAAAC GATGCACGCA GAGAGGTCCA TATAAAAAGA 
ATGGAGGAGA GGAAAGCTTC AAGCACCAGC CCTCCTGGTG ATTCCCTGGC ATCCATCCCT 
TTTATAGATG AGCCAACCAG TCCTAGCATC GACCACGAGA TTGCACATAT TCCTGCATCT 
GCTGTCATCT CCGCCTCTAC TGCCCACGTC CCCTCCATTG CAACTGTTCC CCCCAGCCTC 
ACGACCTCTG CTCCTTTGAT TCGACGTCAG CTCTCCCACG ACCAAGAATC TGTTGGACCT 
CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG ACAGAAAGAT CCAAATCCTA CGATGAAGGA 
CTGGATGATT ATAGAGAAGA TGCAAAACTA TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA 
ATCAAGATTA CAGACAGCCA GAAGTCATCA GAAGACTCTG GATCGAGAAA GGGTTCTTCC 
TCCGAGGTCT TCAGTGATGC AGCCCGAGAA GGCTGGCTCC AGTTCCGACC ACTGGTCACC 
GATAAGGGCA AGAGAGTTGG TGGCAGTATT CGGCCATGGA AACAGATGTA TGTTGTCCTT 
CGGGGCCACT CACTTTACTT GTACAAAGAC AGAAGAGAGC AGACAACGCC ATCAGAAGAA 
GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG ATAGACATCT CGTACAGTGA GACCAAGAGG 
CGGAATGTAT TCCGACTTAC CACGTCTGAC TGTGAATGCT TGTTCCAGGC TGAAGACAGA 
GATGATATGT TATCATGGAT CAAGACTATT CAGGAAAGTA GCAACCTAAA TGAAGAGGAC 
ACTGGAGTTA CCAACAGAGA TCTAATTAGT CGAAGAATCA AAGAGTATAA CAGTCTGCTG 
AGCAGCAAAA CAGAACAGTT GCCAAAAACA CCTCGCCAGA GTCTTAGCAT CAGGCAAACT 
TTGCTTGGTG CTAAATCAGA GCCAAAGACT CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC 
GAGAGGAAGC TTCTCAGTAA AGATGATACC AGTCCCCCTA AAGACAAAGG TACTTGGAGA 
AGAGGCATTC CAAGCATCGT GAGGAAGACG TTTGAGAAAA AGCCGGCCGC CACAGGGACC 
TTTGGAGTTA GGCTGGATGA CTGCCCACCC GCCCACACCA ACAGGTACAT TCCATTAATC 
GTTGACATAT GTTGCAAATT AGTTGAAGAA AGGGGTCTTG AATATACAGG TATTTACCGA 
GTTCCCGGCA ACAACGCAGC CATTTCAAGC ATGCAGGAAG AACTCAACAA GGGGATGGCA 
GACATTGACA TACAAGATGA TAAATGGCGA GATTTGAATG TGATAAGCAG TTTACTAAAA 
TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC TTTACGAATG ACAAATATGC TGACTTCATT 
GAGGCCAATC GCAAAGAGGA CCCACTGGAT CGGCTACGAA CATTAAAGAG ACTAATTCAT 
GACCTGCCTG AGCATCATTT TGAGACGCTC AAGTTTCTCT CGGCTCATCT GAAGACAGTG 
GCAGAGAACT CAGAAAAGAA TAAGATGGAA CCCAGAAACC TAGCGATCGT TTTTGGTCCA 
ACTCTAGTGA GAACATCAGA AGACAACATG ACCCACATGG TCACGCACAT GCCTGACCAG 
TATAAGATCG TAGAGACTCT CATCCAGCAT CATGACTGGT TTTTCACAGA AGAAGGTGCC 
GAAGAGCCTC TTACAGCAGT GCAGGAGGAG AACACAGTAG ACTCCCAGCC AGTACCAAAC 
ATAGATCATT TGCTCACCAA CATTGGAAGG ACAGGCGTGC TCCCTGGAGA TGTATCAGAT 
TCAGCTACTA GTGACTCCGC AAAATCTAAG GGCTCCTGGG GATCCGGGAA GGATCAGTAT 
AGCAGGGAAC TGCTTGTATC CTCCATCTTC GCAGCTGCCA GTCGCAAGAG GAAAAAGCCG 
AAAGAGAAAG CCCAGCCCAG CAGCTCTGAG GATGAGCTGG ACAGTGTGTT CTTTAAGAAG 
GAGAATACGG AACAGAGTCA CAGTGAGATT AAAGAAGAGT CCAAAAGAGA AAGCGAGACA 
TCAGGTAGCA AGCAGAGGGT CGTCGTTGCC AAAGAAAGTA ACACTAAGAA AGACTCGGGT 
ACAACGAAGG AGGAGAAGAA GATACCGTGG GAAGAACCAT CCCCACCACA CAGCTCCAAA 
CGCAACAGGT CACCAACCCT CAGCTGTCGC CTGGCCATGC TGAAAGAGGG CCCCAGATCA 
CTTCTGACAC AGAAGCCCCA CTGTGAAGAG ACAGGGTCTG ACTCAGGCAC TCTGCTTAGC 
ACGTCTTCCC AGGCTTCGCT CTTGAGGTCC TCCACTAAAA AATCCACCAG TCCGGAAACC 
AAGCACAGTG AGTTTTTGTC CATTGCTGGT ACCACCACCT CGGATTATTC TACCACATCA 
TCCACTACCT ACTTGACCAG CCTGGACTCC AGCCGCCTGA GCCCTGAGGT GCAGTCAGTG 
GCCGAGAGCA AGGGGGATGA GGCTGATGAC GAGAGGAGCG AGCTGGTGAG TGAAGGACGG 
CCAGTGGAGA CCGACAGTGA GAGTGAGTTC CCCGTGTTTC CCACCACCCT GACTTCAGAC 
CGGCTGTTCC GAGGAAAATT CCAGGAGGTT GCCAGGGTGA GCCGCAGGAA CTCCGAAGGC 
AGTGAGGCCA GCTGCACCGA GGGAAGCCTC ACACCGAGTT TAGACAGCCG CAGGCAGCAG 
TTCAGCTCCC ATAGACTGAT CGAATGCGAC ACACTGTCCA GGAAGAAGTC TGCTCGCTTC 
AAGTCAGACA GTGGGAGCCC CGGAGACACC AGGACTGAGA AAGAAACTCC TGCCCTGGCC 
AAGATGTTTG ACGTCATGAA GAAAGGCAAG TCGACAGGGA GCCTCCTCAC ACCCAGCAGA 
AGTGAATCCG AAAAGCAGGA GGCCACTTGG AAAACAAAAA TAGCAGATCG TTTAAAACTG 
AGACCCAGGG CACCCGCAGA CGACATGTTT GGAGTTGGGA ATCAAAAACC TACTGCTGAG 
ACAGCGAAAA GGAAAAACAT CAAACGCAGA CACACGCTTG GAGGCCACAG AGATGCTACT 
GAGATCAGCG TCCTGAGTTT CTGGAAAGCT CATGAGCAGA GTGCGGACAA AGAGTCTGAA 
CTTTCGGCTG TGAATCGGTT AAAACCCAAA TGCTCAGCCC AGGACCTTTC CATCTCAGAC 
TGGCTCGCCA GGGAGCGCGT CCGCACCAGT GCCTCCGACC TTAGCAGAGG CGAAGGCCTC 
GAGCCCCAGG CTGAGAGCCC CAGCGTGCTG GGAACACCCA TCAGCACCCA CTCACCACCG 
TCTCAGCAGC CCGAAGCAAG GGTTGCTGCT ACCAGCACCT TGGCTTCAAC CAGCCAGTCC 
CCTCTTTTCA CACCACCACA GTCACCTGAC CAAATAAACA GAGAAAGCTT CCAGAACATG 
AGCCAAAATG CTAGTTCTAC AGCTAACATC CACCCCCACA AACAGTCTGA AAGTCCAGAC 
ACTAAAGCAG AGACTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497607.1
CDS	1896..7739
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

Target ORF information:

RefSeq Version	XM_006497544.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497544.3

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGGCAT ATTCTCAAGA TGCATACCTG 
AAAGGCAATG AAGCTTACAG CGGCAATGCC CGTAATATAC CTGAACCTCC ACCAGTTTGC 
TATCCCTGGT TGCCATCGAC TCCTTCAGCT ACTGCACAGC CGGTGGAAAC ATGCCCTCCT 
GATTCATTAC CGAACAAACA GCAAACCAGT GCACCAGTCC TCACACAACC TGGCAGGGCC 
TATAGAATGG AAATACAAGT GCCTCCGTCA CCAACAGATG TTGCAAAGTC AAACACAGCA 
GTGTGTGTTT GCAATGAGAG TGTGAGGACC GTCATTGTGC CTTCTGAGAA GGTGGTAGAC 
TTGCTAGCCA ATAGAAACAA CCCTTCAGGT CCTTCACATA GAACTGAAGA GGTAAGGTAT 
GGTGTAAATG AGCAGGCCTC TACAAAAGCA GCATCGAGAA CAACGTCACC GGCATCAGTT 
CCCACTGCCC ATCTGATTCA TCAGACCACA GGCTCCAGAT CCTTAGAACC TTCTGGAATT 
TTACTTAAAT CTGGAAATTA CAGTGGACAT TCAGAAGGAA TCTCAAGCAG TAGATCACAA 
GCCGTGGATT CCCCTCCTGT GTCTGTCAAT CACTACTCTG CAAACTCCCA TCAGCACATA 
GACTGGAAAA ATTATAAGAC TTACAAAGAA TACATTGATA ATAGGAGATT GCACATAGGT 
TGTCGGACAA TCCAGGAAAG ACTGGACAGC TTACGAGCTG CATCTCAGAG CGCAGCAGAC 
TATAACCAGG TGGTACCCAC CCGCACTACT CTGCAGGTAC GACGTCGAAG CACCTCTCAT 
GACCGAGTGC CCCAGTCTGT TCAGATCCGG CAGCGCAGTG TATCTCAGGA AAGACTGGAA 
GATTCTGTGC TGATGAAGTA TTGCCCAAGG AGCGCGTCTC AAGGAGCGCT GACCTCACCG 
CCTGTTAGCT TCAATAATCA TAGAACTCGT TCGTGGGATT ACATAGAGGG ACAGACGGAA 
GCCACAGCCA CTGTCAATTC TGAAAGTCAG ATACCTGACT CAAATGGGGA ACGAAAACAG 
ACTTACAAGT GGAGTGGCTT CACTGAGCAG GATGATAGAC GCGGTATTCA CGAAAGACCC 
AGGCAGCAAG AAATGCACAA GCCTTTCCGA GGCTCAAATT TGACTGTGGC TCCCGTTGTT 
AATTCTGATA ACAGGCGCCT GGTTGGGAGA GGGGTAGGAC CTGTCTCACA GTTTAAAAAA 
ATCCCGCCGG ACCTAAGACC ACCACATTCC AACAGAAATT TTCCAACCAC GACTGGGGTG 
TCACTTCAAC GGGGTATTGC ACAAGACAGG TCCCCACTCG TGAAAGTCCG AAGTAATTCT 
CTGAAAGTGC CACCTCCACC TGTGTCAAAA CCGTCCTTCA GCCAGCACTC ACTGGCTTCC 
ATGAAAGACC AAAGGCCTGT CAATCATCTG CATCAACACA GTGTGCTGAG TCAGCAGACG 
CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC CAGCTGGAGA CTGAGGTATC CTCGTGTCTA 
CCAGGAACTT CTGCCAAGAC CAGTCCACAG CTCAGTGAGA ATTTGGGAAC ATCAGACTTA 
GAACTACCTG CCATCCCAAG GAATGGAGAT ATAAACTTAC AGGAGGCTGA AATTCAGCAG 
CCAGATGTGC TAGATAATAA AGAATCTGTC ATTCTAAGAG AGAAACCTCA GTCTGGCCGC 
CAGACGCCAC AGCCTTTAAG ACATCAGTCT TACATCCTGG CAGTGAATGA CCAGGAGACC 
GGGTCAGACA CCACCTGCTG GCTGCCAAAC GATGCACGCA GAGAGGTCCA TATAAAAAGA 
ATGGAGGAGA GGAAAGCTTC AAGCACCAGC CCTCCTGGTG ATTCCCTGGC ATCCATCCCT 
TTTATAGATG AGCCAACCAG TCCTAGCATC GACCACGAGA TTGCACATAT TCCTGCATCT 
GCTGTCATCT CCGCCTCTAC TGCCCACGTC CCCTCCATTG CAACTGTTCC CCCCAGCCTC 
ACGACCTCTG CTCCTTTGAT TCGACGTCAG CTCTCCCACG ACCAAGAATC TGTTGGACCT 
CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG ACAGAAAGAT CCAAATCCTA CGATGAAGGA 
CTGGATGATT ATAGAGAAGA TGCAAAACTA TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA 
ATCAAGATTA CAGACAGCCA GAAGTCATCA GAAGACTCTG GATCGAGAAA GGGTTCTTCC 
TCCGAGGTCT TCAGTGATGC AGCCCGAGAA GGCTGGCTCC AGTTCCGACC ACTGGTCACC 
GATAAGGGCA AGAGAGTTGG TGGCAGTATT CGGCCATGGA AACAGATGTA TGTTGTCCTT 
CGGGGCCACT CACTTTACTT GTACAAAGAC AGAAGAGAGC AGACAACGCC ATCAGAAGAA 
GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG ATAGACATCT CGTACAGTGA GACCAAGAGG 
CGGAATGTAT TCCGACTTAC CACGTCTGAC TGTGAATGCT TGTTCCAGGC TGAAGACAGA 
GATGATATGT TATCATGGAT CAAGACTATT CAGGAAAGTA GCAACCTAAA TGAAGAGGAC 
ACTGGAGTTA CCAACAGAGA TCTAATTAGT CGAAGAATCA AAGAGTATAA CAGTCTGCTG 
AGCAGCAAAA CAGAACAGTT GCCAAAAACA CCTCGCCAGA GTCTTAGCAT CAGGCAAACT 
TTGCTTGGTG CTAAATCAGA GCCAAAGACT CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC 
GAGAGGAAGC TTCTCAGTAA AGATGATACC AGTCCCCCTA AAGACAAAGG TACTTGGAGA 
AGAGGCATTC CAAGCATCGT GAGGAAGACG TTTGAGAAAA AGCCGGCCGC CACAGGGACC 
TTTGGAGTTA GGCTGGATGA CTGCCCACCC GCCCACACCA ACAGGTACAT TCCATTAATC 
GTTGACATAT GTTGCAAATT AGTTGAAGAA AGGGGTCTTG AATATACAGG TATTTACCGA 
GTTCCCGGCA ACAACGCAGC CATTTCAAGC ATGCAGGAAG AACTCAACAA GGGGATGGCA 
GACATTGACA TACAAGATGA TAAATGGCGA GATTTGAATG TGATAAGCAG TTTACTAAAA 
TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC TTTACGAATG ACAAATATGC TGACTTCATT 
GAGGCCAATC GCAAAGAGGA CCCACTGGAT CGGCTACGAA CATTAAAGAG ACTAATTCAT 
GACCTGCCTG AGCATCATTT TGAGACGCTC AAGTTTCTCT CGGCTCATCT GAAGACAGTG 
GCAGAGAACT CAGAAAAGAA TAAGATGGAA CCCAGAAACC TAGCGATCGT TTTTGGTCCA 
ACTCTAGTGA GAACATCAGA AGACAACATG ACCCACATGG TCACGCACAT GCCTGACCAG 
TATAAGATCG TAGAGACTCT CATCCAGCAT CATGACTGGT TTTTCACAGA AGAAGGTGCC 
GAAGAGCCTC TTACAGCAGT GCAGGAGGAG AACACAGTAG ACTCCCAGCC AGTACCAAAC 
ATAGATCATT TGCTCACCAA CATTGGAAGG ACAGGCGTGC TCCCTGGAGA TGTATCAGAT 
TCAGCTACTA GTGACTCCGC AAAATCTAAG GGCTCCTGGG GATCCGGGAA GGATCAGTAT 
AGCAGGGAAC TGCTTGTATC CTCCATCTTC GCAGCTGCCA GTCGCAAGAG GAAAAAGCCG 
AAAGAGAAAG CCCAGCCCAG CAGCTCTGAG GATGAGCTGG ACAGTGTGTT CTTTAAGAAG 
GAGAATACGG AACAGAGTCA CAGTGAGATT AAAGAAGAGT CCAAAAGAGA AAGCGAGACA 
TCAGGTAGCA AGCAGAGGGT CGTCGTTGCC AAAGAAAGTA ACACTAAGAA AGACTCGGGT 
ACAACGAAGG AGGAGAAGAA GATACCGTGG GAAGAACCAT CCCCACCACA CAGCTCCAAA 
CGCAACAGGT CACCAACCCT CAGCTGTCGC CTGGCCATGC TGAAAGAGGG CCCCAGATCA 
CTTCTGACAC AGAAGCCCCA CTGTGAAGAG ACAGGGTCTG ACTCAGGCAC TCTGCTTAGC 
ACGTCTTCCC AGGCTTCGCT CTTGAGGTCC TCCACTAAAA AATCCACCAG TCCGGAAACC 
AAGCACAGTG AGTTTTTGTC CATTGCTGGT ACCACCACCT CGGATTATTC TACCACATCA 
TCCACTACCT ACTTGACCAG CCTGGACTCC AGCCGCCTGA GCCCTGAGGT GCAGTCAGTG 
GCCGAGAGCA AGGGGGATGA GGCTGATGAC GAGAGGAGCG AGCTGGTGAG TGAAGGACGG 
CCAGTGGAGA CCGACAGTGA GAGTGAGTTC CCCGTGTTTC CCACCACCCT GACTTCAGAC 
CGGCTGTTCC GAGGAAAATT CCAGGAGGTT GCCAGGGTGA GCCGCAGGAA CTCCGAAGGC 
AGTGAGGCCA GCTGCACCGA GGGAAGCCTC ACACCGAGTT TAGACAGCCG CAGGCAGCAG 
TTCAGCTCCC ATAGACTGAT CGAATGCGAC ACACTGTCCA GGAAGAAGTC TGCTCGCTTC 
AAGTCAGACA GTGGGAGCCC CGGAGACACC AGGACTGAGA AAGAAACTCC TGCCCTGGCC 
AAGATGTTTG ACGTCATGAA GAAAGGCAAG TCGACAGGGA GCCTCCTCAC ACCCAGCAGA 
AGTGAATCCG AAAAGCAGGA GGCCACTTGG AAAACAAAAA TAGCAGATCG TTTAAAACTG 
AGACCCAGGG CACCCGCAGA CGACATGTTT GGAGTTGGGA ATCAAAAACC TACTGCTGAG 
ACAGCGAAAA GGAAAAACAT CAAACGCAGA CACACGCTTG GAGGCCACAG AGATGCTACT 
GAGATCAGCG TCCTGAGTTT CTGGAAAGCT CATGAGCAGA GTGCGGACAA AGAGTCTGAA 
CTTTCGGCTG TGAATCGGTT AAAACCCAAA TGCTCAGCCC AGGACCTTTC CATCTCAGAC 
TGGCTCGCCA GGGAGCGCGT CCGCACCAGT GCCTCCGACC TTAGCAGAGG CGAAGGCCTC 
GAGCCCCAGG CTGAGAGCCC CAGCGTGCTG GGAACACCCA TCAGCACCCA CTCACCACCG 
TCTCAGCAGC CCGAAGCAAG GGTTGCTGCT ACCAGCACCT TGGCTTCAAC CAGCCAGTCC 
CCTCTTTTCA CACCACCACA GTCACCTGAC CAAATAAACA GAGAAAGCTT CCAGAACATG 
AGCCAAAATG CTAGTTCTAC AGCTAACATC CACCCCCACA AACAGTCTGA AAGTCCAGAC 
ACTAAAGCAG AGACTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap21 ( XM_011238986.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237288.1 Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X9, mRNA.

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CloneID	OMu70706
Clone ID Related Accession (Same CDS sequence)	XM_011238986.2 , XM_011238986.3
Accession Version	XM_011238986.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 21 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAATTCAT GACCTGCCTG AGCATCATTT TGAGACGCTC 
AAGTTTCTCT CGGCTCATCT GAAGACAGTG GCAGAGAACT CAGAAAAGAA TAAGATGGAA 
CCCAGAAACC TAGCGATCGT TTTTGGTCCA ACTCTAGTGA GAACATCAGA AGACAACATG 
ACCCACATGG TCACGCACAT GCCTGACCAG TATAAGATCG TAGAGACTCT CATCCAGCAT 
CATGACTGGT TTTTCACAGA AGAAGGTGCC GAAGAGCCTC TTACAGCAGT GCAGGAGGAG 
AACACAGTAG ACTCCCAGCC AGTACCAAAC ATAGATCATT TGCTCACCAA CATTGGAAGG 
ACAGGCGTGC TCCCTGGAGA TGTATCAGAT TCAGCTACTA GTGACTCCGC AAAATCTAAG 
GGTCTCACTG TGGAGTTGGG GCAGCCTTGG AGCTTGTCCT CTTCCTGGCA GCCTACCCGG 
CACTGTGACT ATAGGTGTAT ACCATCACAC CTGGCAGAAC TCTTCTACTT GTACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237288.1
CDS	11405..15724
Translation	MAQFRLLDCMINHNWSLLCNLYCAWNGQVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGLTVELGQPWSLSSSWQPTRHCDYRCIPSHLAELFYLYI

Target ORF information:

RefSeq Version	XM_011238986.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 21 (Arhgap21), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238986.2

ORF Insert Sequence:

ATGGCCCAGT TCAGGCTGCT GGACTGCATG ATAAACCACA ATTGGAGTTT ACTCTGCAAT 
CTCTACTGTG CATGGAATGG CCAGGTGTCA AAAAACAAAG ATGGAAAAGA CCAAGGTGAA 
CCTGTGTCAC CGTCTGAAGA TGAGCCATTC TCCTGGCCAG GTCCCAAAAC AGTCATGCTG 
AAGAGAACAT CTCAAGGGTT TGGTTTCACA TTACGACACT TCATCGTTTA TCCCCCAGAG 
TCTGCAATCC AGTTTTCATA TAAGGATGAA GAGAATGGAA ACAGAGGAGG AAAGCAAAGG 
AACCGATTGG AGCCGATGGA TACCATATTT GTTAAACAAG TGAAGGAAGG AGGGCCGGCC 
TTCGAGGCTG GCTTATGCAC AGGTGACAGA ATTATAAAAG TCAATGGAGA AAGTGTCATA 
GGAAAGACCT ATTCCCAAGT AATTGCTCTC ATTCAGAACA GTGACACCAC CCTGGAGCTC 
AGTGTAATGC CCAAGGATGA AGACATTCTC CAAGTGCTAC AATTTACAAA GGATGTTACA 
GCACTGGCAT ATTCTCAAGA TGCATACCTG AAAGGCAATG AAGCTTACAG CGGCAATGCC 
CGTAATATAC CTGAACCTCC ACCAGTTTGC TATCCCTGGT TGCCATCGAC TCCTTCAGCT 
ACTGCACAGC CGGTGGAAAC ATGCCCTCCT GATTCATTAC CGAACAAACA GCAAACCAGT 
GCACCAGTCC TCACACAACC TGGCAGGGCC TATAGAATGG AAATACAAGT GCCTCCGTCA 
CCAACAGATG TTGCAAAGTC AAACACAGCA GTGTGTGTTT GCAATGAGAG TGTGAGGACC 
GTCATTGTGC CTTCTGAGAA GGTGGTAGAC TTGCTAGCCA ATAGAAACAA CCCTTCAGGT 
CCTTCACATA GAACTGAAGA GGTAAGGTAT GGTGTAAATG AGCAGGCCTC TACAAAAGCA 
GCATCGAGAA CAACGTCACC GGCATCAGTT CCCACTGCCC ATCTGATTCA TCAGACCACA 
GGCTCCAGAT CCTTAGAACC TTCTGGAATT TTACTTAAAT CTGGAAATTA CAGTGGACAT 
TCAGAAGGAA TCTCAAGCAG TAGATCACAA GCCGTGGATT CCCCTCCTGT GTCTGTCAAT 
CACTACTCTG CAAACTCCCA TCAGCACATA GACTGGAAAA ATTATAAGAC TTACAAAGAA 
TACATTGATA ATAGGAGATT GCACATAGGT TGTCGGACAA TCCAGGAAAG ACTGGACAGC 
TTACGAGCTG CATCTCAGAG CGCAGCAGAC TATAACCAGG TGGTACCCAC CCGCACTACT 
CTGCAGGTAC GACGTCGAAG CACCTCTCAT GACCGAGTGC CCCAGTCTGT TCAGATCCGG 
CAGCGCAGTG TATCTCAGGA AAGACTGGAA GATTCTGTGC TGATGAAGTA TTGCCCAAGG 
AGCGCGTCTC AAGGAGCGCT GACCTCACCG CCTGTTAGCT TCAATAATCA TAGAACTCGT 
TCGTGGGATT ACATAGAGGG ACAGACGGAA GCCACAGCCA CTGTCAATTC TGAAAGTCAG 
ATACCTGACT CAAATGGGGA ACGAAAACAG ACTTACAAGT GGAGTGGCTT CACTGAGCAG 
GATGATAGAC GCGGTATTCA CGAAAGACCC AGGCAGCAAG AAATGCACAA GCCTTTCCGA 
GGCTCAAATT TGACTGTGGC TCCCGTTGTT AATTCTGATA ACAGGCGCCT GGTTGGGAGA 
GGGGTAGGAC CTGTCTCACA GTTTAAAAAA ATCCCGCCGG ACCTAAGACC ACCACATTCC 
AACAGAAATT TTCCAACCAC GACTGGGGTG TCACTTCAAC GGGGTATTGC ACAAGACAGG 
TCCCCACTCG TGAAAGTCCG AAGTAATTCT CTGAAAGTGC CACCTCCACC TGTGTCAAAA 
CCGTCCTTCA GCCAGCACTC ACTGGCTTCC ATGAAAGACC AAAGGCCTGT CAATCATCTG 
CATCAACACA GTGTGCTGAG TCAGCAGACG CAGTTCAGAT CCGAAAGCAC CTTCGAGCAC 
CAGCTGGAGA CTGAGGTATC CTCGTGTCTA CCAGGAACTT CTGCCAAGAC CAGTCCACAG 
CTCAGTGAGA ATTTGGGAAC ATCAGACTTA GAACTACCTG CCATCCCAAG GAATGGAGAT 
ATAAACTTAC AGGAGGCTGA AATTCAGCAG CCAGATGTGC TAGATAATAA AGAATCTGTC 
ATTCTAAGAG AGAAACCTCA GTCTGGCCGC CAGACGCCAC AGCCTTTAAG ACATCAGTCT 
TACATCCTGG CAGTGAATGA CCAGGAGACC GGGTCAGACA CCACCTGCTG GCTGCCAAAC 
GATGCACGCA GAGAGGTCCA TATAAAAAGA ATGGAGGAGA GGAAAGCTTC AAGCACCAGC 
CCTCCTGGTG ATTCCCTGGC ATCCATCCCT TTTATAGATG AGCCAACCAG TCCTAGCATC 
GACCACGAGA TTGCACATAT TCCTGCATCT GCTGTCATCT CCGCCTCTAC TGCCCACGTC 
CCCTCCATTG CAACTGTTCC CCCCAGCCTC ACGACCTCTG CTCCTTTGAT TCGACGTCAG 
CTCTCCCACG ACCAAGAATC TGTTGGACCT CCCAGCCTGG ATGGCCAGCA CAGCTCAAAG 
ACAGAAAGAT CCAAATCCTA CGATGAAGGA CTGGATGATT ATAGAGAAGA TGCAAAACTA 
TCCTTTAAGC ACGTGTCTAG CCTGAAGGGA ATCAAGATTA CAGACAGCCA GAAGTCATCA 
GAAGACTCTG GATCGAGAAA GGGTTCTTCC TCCGAGGTCT TCAGTGATGC AGCCCGAGAA 
GGCTGGCTCC AGTTCCGACC ACTGGTCACC GATAAGGGCA AGAGAGTTGG TGGCAGTATT 
CGGCCATGGA AACAGATGTA TGTTGTCCTT CGGGGCCACT CACTTTACTT GTACAAAGAC 
AGAAGAGAGC AGACAACGCC ATCAGAAGAA GAGCAGCCCA TCAGTGTCAA CGCTTGCCTG 
ATAGACATCT CGTACAGTGA GACCAAGAGG CGGAATGTAT TCCGACTTAC CACGTCTGAC 
TGTGAATGCT TGTTCCAGGC TGAAGACAGA GATGATATGT TATCATGGAT CAAGACTATT 
CAGGAAAGTA GCAACCTAAA TGAAGAGGAC ACTGGAGTTA CCAACAGAGA TCTAATTAGT 
CGAAGAATCA AAGAGTATAA CAGTCTGCTG AGCAGCAAAA CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTTAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ATTCTCCAAA GGAGGAGTCC GAGAGGAAGC TTCTCAGTAA AGATGATACC 
AGTCCCCCTA AAGACAAAGG TACTTGGAGA AGAGGCATTC CAAGCATCGT GAGGAAGACG 
TTTGAGAAAA AGCCGGCCGC CACAGGGACC TTTGGAGTTA GGCTGGATGA CTGCCCACCC 
GCCCACACCA ACAGGTACAT TCCATTAATC GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGGGGTCTTG AATATACAGG TATTTACCGA GTTCCCGGCA ACAACGCAGC CATTTCAAGC 
ATGCAGGAAG AACTCAACAA GGGGATGGCA GACATTGACA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAGCTCCC TGAGCCTCTC 
TTTACGAATG ACAAATATGC TGACTTCATT GAGGCCAATC GCAAAGAGGA CCCACTGGAT 
CGGCTACGAA CATTAAAGAG ACTAATTCAT GACCTGCCTG AGCATCATTT TGAGACGCTC 
AAGTTTCTCT CGGCTCATCT GAAGACAGTG GCAGAGAACT CAGAAAAGAA TAAGATGGAA 
CCCAGAAACC TAGCGATCGT TTTTGGTCCA ACTCTAGTGA GAACATCAGA AGACAACATG 
ACCCACATGG TCACGCACAT GCCTGACCAG TATAAGATCG TAGAGACTCT CATCCAGCAT 
CATGACTGGT TTTTCACAGA AGAAGGTGCC GAAGAGCCTC TTACAGCAGT GCAGGAGGAG 
AACACAGTAG ACTCCCAGCC AGTACCAAAC ATAGATCATT TGCTCACCAA CATTGGAAGG 
ACAGGCGTGC TCCCTGGAGA TGTATCAGAT TCAGCTACTA GTGACTCCGC AAAATCTAAG 
GGTCTCACTG TGGAGTTGGG GCAGCCTTGG AGCTTGTCCT CTTCCTGGCA GCCTACCCGG 
CACTGTGACT ATAGGTGTAT ACCATCACAC CTGGCAGAAC TCTTCTACTT GTACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Arhgap21
Entrez Gene ID	71435
Full Name	Rho GTPase activating protein 21
Synonyms	5530401C11Rik,AA416458,ARHGAP10
General protein information	Preferred Names Rho GTPase activating protein 21 Names rho GTPase-activating protein 21 rho GTPase-activating protein 10 rho-type GTPase-activating protein 21
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A3

	ARHGAP21	XP_001100413.2
Bos taurus (cattle)	ARHGAP21	XP_005214355.1
Gallus gallus (chicken)	ARHGAP21	NP_001170828.1
Canis lupus familiaris (dog)	ARHGAP21	XP_005617050.1
Mus musculus (house mouse)	Arhgap21	NP_001121556.2
Danio rerio (zebrafish)	arhgap21b	XP_005163349.1
Pan troglodytes (chimpanzee)	ARHGAP21	XP_507699.3
Xenopus tropicalis (tropical clawed frog)	arhgap21	NP_001090761.1
Homo sapiens (human)	ARHGAP21	NP_065875.3
Rattus norvegicus (Norway rat)	Arhgap21	NP_001178622.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Arhgap21 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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