Spef1 cDNA ORF clone, Mus musculus(house mouse)

The following Spef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu04454 NM_027641.2
Latest version!
Mus musculus sperm flagellar 1 (Spef1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $188.30
$269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu04454
    Clone ID Related Accession (Same CDS sequence) NM_027641.2
    Accession Version NM_027641.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-19
    Organism Mus musculus(house mouse)
    Product sperm flagellar protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK047675.1 and AK046964.1. On Sep 16, 2006 this sequence version replaced NM_027641.1. ##Evidence-Data-START## Transcript exon combination :: AK047675.1, SRR1660815.208702.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAAAGCA GCGTGGATGA GGAGGCTCTG CACCAGCTGT ACCTCTGGGT AGACAATATC 
    CCTCTGTCCA GACCCAAGAG AAACCTCTCC CGGGACTTCA GTGACGGAGT CCTGGTTGCA
    GAGCTTATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCTGCCAAC
    TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CACCTGAACA GAAAGGTGCT GAACAAGCTG
    AACTTCTCGG TCCCTGACGA TGTGATGCGG AAGATTGCGC AGTGTTCTCC GGGAGTAGTG
    GAGCTAGTGC TCATTCCACT GAGGCAGCGC CTGGAGGAGC GGCAGAGGCG CCAGAAGCTG
    GGTGTTGGCT CCTTACAGGA GCTGGCTCCT CAGGATAGCA GTGGCTACAT GGATATGGGT
    TTGCCCCAGA AGGTTCGAGG AGAGGGTGCC CCAGCCTTGG GAGAGCAGCT CAGAGAGGGC
    CGTCCGTTGG CTTCCCGACC CCCTGGGTAT AACCAGGCGC TGCAGGGTGA TCCGAGCTTC
    GTCCTCCAGA TCGCTGAAAA GGAGCAGGAG CTGTTGGCCT CTCAAGAGAC AGTACAGGTC
    CTTCAGATGA AGGTGAAGCG TCTGGAACAT CTGCTCCAAC TTAAGAACGT GCGCATCGAT
    GATCTCTCCC GGCGGCTCCA GCAGGCCGAG CGCAAGCAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_081917.1
    CDS138..842
    Translation

    Target ORF information:

    RefSeq Version NM_027641.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sperm flagellar 1 (Spef1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_027641.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAAAGCA GCGTGGATGA GGAGGCTCTG CACCAGCTGT ACCTCTGGGT AGACAATATC 
    CCTCTGTCCA GACCCAAGAG AAACCTCTCC CGGGACTTCA GTGACGGAGT CCTGGTTGCA
    GAGCTTATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCTGCCAAC
    TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CACCTGAACA GAAAGGTGCT GAACAAGCTG
    AACTTCTCGG TCCCTGACGA TGTGATGCGG AAGATTGCGC AGTGTTCTCC GGGAGTAGTG
    GAGCTAGTGC TCATTCCACT GAGGCAGCGC CTGGAGGAGC GGCAGAGGCG CCAGAAGCTG
    GGTGTTGGCT CCTTACAGGA GCTGGCTCCT CAGGATAGCA GTGGCTACAT GGATATGGGT
    TTGCCCCAGA AGGTTCGAGG AGAGGGTGCC CCAGCCTTGG GAGAGCAGCT CAGAGAGGGC
    CGTCCGTTGG CTTCCCGACC CCCTGGGTAT AACCAGGCGC TGCAGGGTGA TCCGAGCTTC
    GTCCTCCAGA TCGCTGAAAA GGAGCAGGAG CTGTTGGCCT CTCAAGAGAC AGTACAGGTC
    CTTCAGATGA AGGTGAAGCG TCTGGAACAT CTGCTCCAAC TTAAGAACGT GCGCATCGAT
    GATCTCTCCC GGCGGCTCCA GCAGGCCGAG CGCAAGCAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    High-throughput discovery of novel developmental phenotypes.
    Nature537(7621)508-514(2016 09)
    Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA


    The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
    Nucleic acids research42(Database issue)D802-9(2014 Jan)
    Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H


    CLAMP, a novel microtubule-associated protein with EB-type calponin homology.
    Cell motility and the cytoskeleton62(3)141-56(2005 Nov)
    Dougherty GW,Adler HJ,Rzadzinska A,Gimona M,Tomita Y,Lattig MC,Merritt RC Jr,Kachar B


    Spef1, a conserved novel testis protein found in mouse sperm flagella.
    Gene353(2)189-99(2005 Jul)
    Chan SW,Fowler KJ,Choo KH,Kalitsis P