Nol8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nol8
Entrez Gene ID	70930
Full Name	nucleolar protein 8
Synonyms	4921532D18Rik,5730412B09Rik
General protein information	Preferred Names nucleolar protein 8 Names nucleolar protein 8 nol8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 A5\|13 25.36 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006516957.1	XP_006517020.1	nucleolar protein 8 isoform X2
XM_006516958.4	XP_006517021.1	nucleolar protein 8 isoform X2
XM_006516958.3	XP_006517021.1	nucleolar protein 8 isoform X2
NM_001271397.1	NP_001258326.1	nucleolar protein 8
XM_006516956.3	XP_006517019.2	nucleolar protein 8 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NOL8	NP_060418.4
Pan troglodytes (chimpanzee)	NOL8	XP_003312216.2
Bos taurus (cattle)	LOC101905441	XP_005218486.1
Rattus norvegicus (Norway rat)	Nol8	XP_006253800.1
	NOL8	XP_001102499.1
Canis lupus familiaris (dog)	NOL8	XP_003432734.1
Bos taurus (cattle)	NOL8	NP_001178142.1
Mus musculus (house mouse)	Nol8	NP_001258326.1
Gallus gallus (chicken)	NOL8	XP_004944563.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
Tarantino C, Paolella G, Cozzuto L, Minopoli G, Pastore L, Parisi S, Russo T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nol8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nol8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06965	XM_006516958.4 Latest version!	Mus musculus nucleolar protein 8 (Nol8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu06965	NM_001271397.1 Latest version!	Mus musculus nucleolar protein 8 (Nol8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu06965	XM_006516957.1 Latest version!	Mus musculus nucleolar protein 8 (Nol8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu70599	XM_006516956.3 Latest version!	Mus musculus nucleolar protein 8 (Nol8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu06965	XM_006516958.3	Mus musculus nucleolar protein 8 (Nol8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nol8 ( XM_006516958.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517021.1 Mus musculus nucleolar protein 8 (Nol8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu06965
Clone ID Related Accession (Same CDS sequence)	XM_006516957.1 , XM_006516958.4 , XM_006516958.3 , NM_001271397.1
Accession Version	XM_006516958.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nucleolar protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516958.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAC CTCTGGAAAA CGTTTCCAGC AATCTTGATA GATGCCCAAC ATCCATGCAA 
GGCAACAGAG AAATGAAGCG CCTCTTTGTG GGTGGACTTG GACAGGGCAT TTCTGAGACA 
GACCTACAAA ATCAGTTCGG CAGATTTGGA GAAGTTTCTG ACGTGGAGAT CATCACTCGG 
AAAGATGACC AAGGAAATTC ACAGAAAGTC TTTGCGTATG TTAACATTCA AATAACAGAA 
GCGGACCTGA AGAAATGTAT GTCTATTTTA AACAAAACAA AATGGAAAGG TGGAACACTA 
CAAATTCAGC TAGCAAAAGA AAGCTTTTTA CACAGATTGG CCCAAGAAAG AGAAGACGCA 
AAAGCAAAGA AAGAAAAATC AACAACAGGC AACCCCACCT TATTGGAAAA GATGGGAGCA 
GTGGATTTCC ATATGAAGGC TGTGCCTGGA ACAGAAGTAC CAGGGCATAA AAATTGGGTT 
GTGAGTAAGT TTGGAAGAGT CTTACCTGTT CTTCACCTTA AGAATCAACA AAAACATAAA 
ATTATGAAAT ATGATCCATC AAAATACTGC CACAATATAA AAAAGATTCC AGAGAATTTG 
ACAGAAACCA CTCCCATAGC TGAACTCACT TGGGAATTGG AAGGAGGCAA TGACCCTATG 
AGTAAGAAAC GTCGAGGAGA GTTCTCTGAC TTTCATATCC CTCCTCAAAA GGTCAAGAAA 
GTGCAGAAGA GCAATGATCC CATGGAGTCC AAGGTTTCTA ATATTGGTCT AAGGACTAAT 
CAGGTAATGG AGAAGAACAA ATCAACACAT CCTGTGACTG CACACGGGAC AGCCCCTAGT 
ACTGTTAATC CTTCTAAACA ACTCCTTGTG TCCAGTTCTG GTACTCAGAA ACCTAAACAT 
GTGGTTTTTC ATAATTCTGA CTTTGAAATT ATCTGGAATA AAAGTAGCAT GTCTGATGAT 
GATGTTGACT CGGAAGATGA ATTAAAAATG ATGATTGCAA AAGAGGAAAA CAGAGAGAAA 
CCTGGGCATT CCTCAGTCAA TGAGTCTGAA CATGATACTT TTGAAGTTGT TAGGGATGAT 
TTCAAATCAA ATATTCACAA GCTTTCTTCT TCAGTAAGCC TAGGAAATAA CCATGAATAT 
GATTCAAGTG ATACAGATGA AATTATTGCA ATGAAAACAA AAAATGCTAA GGTCAAAAAC 
AGTGCAGAAT CTTCACAACC AGAAAGGACT GTAAGCAAGA AAAGTTCTTT TCAAAAGATA 
GAACCTTCTA ATGACTGTAT AAAAGTACAA GGAATTAACA GCAACAAAGA ATCAGCCCTG 
TGTCATGGAG TAAAGTTTGT AAATCCCAAA TTTCCACCTG ACTCCAGTGG CAGTGACAGT 
GAAGAGTCCG AAGAAGATGA AGAATATAAA GTCTTGATGG AAAACTGTCC CCGTGTGAGT 
CTCACTTTGG CTGACTTGGA GCAGTTGGCT GGCAGTCATC GGAAGTTTCC AGGAAAAGAC 
AGTGAGACTA ATGGTCCACA AAATGACAGT CACTGCAAAT TTGACACAAC CTCCAAGAAT 
CCCAAGACTT CTGGTGACCT GTACAATGGC AGACAACAGT GCATTCTTCC TGAGGAGATT 
GTGGCCTCCC TTTTAGAGGA CGAGAATACT TACAGCAAAC AAAAATCGGA AGAAGATATC 
TTAAAGCCAA AATTCCAGGC CTTCAAGGGA ATAGGCTGTC TCTATGCAAA GGAGTCAGTA 
GACAAAACTT TGAAAGAGAA TATTGCCTTT AATACTGGCG GTGGGCATCA CAGTTCCTTA 
AAACATGAAG ATCACAATAG AAGTTTGATG GAAAATGGAT CTAAGTGTGT TAATGGCTCA 
TCAAGCAAAC TGACTTCATG CCAACCTGCA AAGAAGGTGA ATGACCCGAA CCACATTCAG 
CCTCCAAAAA GACAGTGTAC TTTTGAGAAC CAGAACCATA AAGTAATGTC CTCTACTAGT 
TGTGACAAGG GAAGTACAAA TCCTCTCCCA GGTCCGTTGC CATTGAAAGC TAAAACATCC 
CTGCATCTTA GTGCCAACAG TCACAAGGTA GACTCTGATG GAGACGCTTG CCATTGGCCT 
GAAAGTAGAA AAGCTTTAGA GAAAGAGAGG ACCAATTTAA GCAACCTCGA ATCTCTTGAG 
AAATCATCAA AGGTGTCTCC CAGGGAAGAC CCTCAGAAAA GCCCAGCTGG TTTCTCACTT 
TCTGATAGTA ATGCATCCTG TATAAATGCT AAGGATAAAC AAGCTGAAGA CAACCAGAAG 
CGATTGGCCG CCTTGGCAGC ATGGCAGAAA GCCAGGGAAG TACAGAAAAA ACTGGTGCAT 
AGTGCCCTGG CAAATTTGGA TGGTCATCCA GAGGACAAAA AAACACACAT TGTCTTTGCT 
TCTGATAATG AAAGTGAAAC GGAAGAGACA TCCACTCAGG AGCAAAGCTG TCCAGAAAAG 
GAGCTGATGA AAGAATCTGT GAGTAAATCG CCTGGGAAGC TGTTTGACAG CAGTGATGAT 
GAAGACTCAG ATTCCAAGGA GGACAGCACC AGGTTCAGCA TTAAACCTCA GTTTGAGGGC 
AGAGCTGGCC AGAAGCTTAT GGATCTGCAG TCTCAGTTTG GAAGTGATGA GAGATTCCGC 
ATGGACTCTA GATTTTTAGA GAGTGACAGT GAGGACGAAA AGAAAGAGCT AAATGAAGAT 
AAAGTGAATG AAGACGAGCT TGCTGCAGAA AAAAAGAAAA CCCTGAATGT TGTGCAAAGT 
GTTTTGAACA TCAATGTGAA CAACCCGACG AACAAAGGAT CAGTGGCTGC TAAGAAATTT 
AAGGATATCG TACATTATGA CCCAACAAAG CATGACCATG CCATTTATGA AAGAAAACAA 
GAAGATAAAG AAAAAGAAAG TAAGGCAACA CGGAAGAAGA AAAAGGAAGA AGCAGAGAAG 
CTGCCTGAGG TATCCCAAGA CATGTATTAT AACATTGCTG CAGATTTGAA AGAAATATTC 
CAAAGTATGA GCAACACAGA TGAAAAGGAA GAAGATGTGC CCAGGACTGA GGCTGGTGCT 
AGAGAGGGGA CTGGAAAAAT CAGGAACGCC GAGACACTGG CATGTGAGCC CGAGCAAACC 
ACTGGCTTTA CATTCTCTTT TTTTGATTCA GCCACTAAAG ATGAAAAGGA TGCCACCTAT 
AGAATCGAAC TAGTGAAACA TGGGAAGATA GTCTGTCCAA ATGATCCCCG ATTCCAAGAC 
AGTAGTTCAG AAGAAGAAGA TATTGCTGAA GAAGCAGATC ATAGCAAGCC AAGCCCTGGA 
GAAGCGGTTC CTGAGAATGA GGCCATTAGA TTTTTCTTTT TCTCTGAGAA TGACGACAGA 
CTTCGTGGTT CTAACTTATT CTGGAGTGGT ATGGGAGGTA GTATTAGCAG AAATTCTTGG 
GAAGCCCGAA CAAGTAGTCT CCTTTTGGAA TGTCGGAAGA AACATAAAGA AGCCAAAAGG 
AAGGTGAAAG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517021.1
CDS	149..3646
Translation	MERPLENVSSNLDRCPTSMQGNREMKRLFVGGLGQGISETDLQNQFGRFGEVSDVEIITRKDDQGNSQKVFAYVNIQITEADLKKCMSILNKTKWKGGTLQIQLAKESFLHRLAQEREDAKAKKEKSTTGNPTLLEKMGAVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQQKHKIMKYDPSKYCHNIKKIPENLTETTPIAELTWELEGGNDPMSKKRRGEFSDFHIPPQKVKKVQKSNDPMESKVSNIGLRTNQVMEKNKSTHPVTAHGTAPSTVNPSKQLLVSSSGTQKPKHVVFHNSDFEIIWNKSSMSDDDVDSEDELKMMIAKEENREKPGHSSVNESEHDTFEVVRDDFKSNIHKLSSSVSLGNNHEYDSSDTDEIIAMKTKNAKVKNSAESSQPERTVSKKSSFQKIEPSNDCIKVQGINSNKESALCHGVKFVNPKFPPDSSGSDSEESEEDEEYKVLMENCPRVSLTLADLEQLAGSHRKFPGKDSETNGPQNDSHCKFDTTSKNPKTSGDLYNGRQQCILPEEIVASLLEDENTYSKQKSEEDILKPKFQAFKGIGCLYAKESVDKTLKENIAFNTGGGHHSSLKHEDHNRSLMENGSKCVNGSSSKLTSCQPAKKVNDPNHIQPPKRQCTFENQNHKVMSSTSCDKGSTNPLPGPLPLKAKTSLHLSANSHKVDSDGDACHWPESRKALEKERTNLSNLESLEKSSKVSPREDPQKSPAGFSLSDSNASCINAKDKQAEDNQKRLAALAAWQKAREVQKKLVHSALANLDGHPEDKKTHIVFASDNESETEETSTQEQSCPEKELMKESVSKSPGKLFDSSDDEDSDSKEDSTRFSIKPQFEGRAGQKLMDLQSQFGSDERFRMDSRFLESDSEDEKKELNEDKVNEDELAAEKKKTLNVVQSVLNINVNNPTNKGSVAAKKFKDIVHYDPTKHDHAIYERKQEDKEKESKATRKKKKEEAEKLPEVSQDMYYNIAADLKEIFQSMSNTDEKEEDVPRTEAGAREGTGKIRNAETLACEPEQTTGFTFSFFDSATKDEKDATYRIELVKHGKIVCPNDPRFQDSSSEEEDIAEEADHSKPSPGEAVPENEAIRFFFFSENDDRLRGSNLFWSGMGGSISRNSWEARTSSLLLECRKKHKEAKRKVKAN

Target ORF information:

RefSeq Version	XM_006516958.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleolar protein 8 (Nol8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516958.4

ORF Insert Sequence:

ATGGAGCGAC CTCTGGAAAA CGTTTCCAGC AATCTTGATA GATGCCCAAC ATCCATGCAA 
GGCAACAGAG AAATGAAGCG CCTCTTTGTG GGTGGACTTG GACAGGGCAT TTCTGAGACA 
GACCTACAAA ATCAGTTCGG CAGATTTGGA GAAGTTTCTG ACGTGGAGAT CATCACTCGG 
AAAGATGACC AAGGAAATTC ACAGAAAGTC TTTGCGTATG TTAACATTCA AATAACAGAA 
GCGGACCTGA AGAAATGTAT GTCTATTTTA AACAAAACAA AATGGAAAGG TGGAACACTA 
CAAATTCAGC TAGCAAAAGA AAGCTTTTTA CACAGATTGG CCCAAGAAAG AGAAGACGCA 
AAAGCAAAGA AAGAAAAATC AACAACAGGC AACCCCACCT TATTGGAAAA GATGGGAGCA 
GTGGATTTCC ATATGAAGGC TGTGCCTGGA ACAGAAGTAC CAGGGCATAA AAATTGGGTT 
GTGAGTAAGT TTGGAAGAGT CTTACCTGTT CTTCACCTTA AGAATCAACA AAAACATAAA 
ATTATGAAAT ATGATCCATC AAAATACTGC CACAATATAA AAAAGATTCC AGAGAATTTG 
ACAGAAACCA CTCCCATAGC TGAACTCACT TGGGAATTGG AAGGAGGCAA TGACCCTATG 
AGTAAGAAAC GTCGAGGAGA GTTCTCTGAC TTTCATATCC CTCCTCAAAA GGTCAAGAAA 
GTGCAGAAGA GCAATGATCC CATGGAGTCC AAGGTTTCTA ATATTGGTCT AAGGACTAAT 
CAGGTAATGG AGAAGAACAA ATCAACACAT CCTGTGACTG CACACGGGAC AGCCCCTAGT 
ACTGTTAATC CTTCTAAACA ACTCCTTGTG TCCAGTTCTG GTACTCAGAA ACCTAAACAT 
GTGGTTTTTC ATAATTCTGA CTTTGAAATT ATCTGGAATA AAAGTAGCAT GTCTGATGAT 
GATGTTGACT CGGAAGATGA ATTAAAAATG ATGATTGCAA AAGAGGAAAA CAGAGAGAAA 
CCTGGGCATT CCTCAGTCAA TGAGTCTGAA CATGATACTT TTGAAGTTGT TAGGGATGAT 
TTCAAATCAA ATATTCACAA GCTTTCTTCT TCAGTAAGCC TAGGAAATAA CCATGAATAT 
GATTCAAGTG ATACAGATGA AATTATTGCA ATGAAAACAA AAAATGCTAA GGTCAAAAAC 
AGTGCAGAAT CTTCACAACC AGAAAGGACT GTAAGCAAGA AAAGTTCTTT TCAAAAGATA 
GAACCTTCTA ATGACTGTAT AAAAGTACAA GGAATTAACA GCAACAAAGA ATCAGCCCTG 
TGTCATGGAG TAAAGTTTGT AAATCCCAAA TTTCCACCTG ACTCCAGTGG CAGTGACAGT 
GAAGAGTCCG AAGAAGATGA AGAATATAAA GTCTTGATGG AAAACTGTCC CCGTGTGAGT 
CTCACTTTGG CTGACTTGGA GCAGTTGGCT GGCAGTCATC GGAAGTTTCC AGGAAAAGAC 
AGTGAGACTA ATGGTCCACA AAATGACAGT CACTGCAAAT TTGACACAAC CTCCAAGAAT 
CCCAAGACTT CTGGTGACCT GTACAATGGC AGACAACAGT GCATTCTTCC TGAGGAGATT 
GTGGCCTCCC TTTTAGAGGA CGAGAATACT TACAGCAAAC AAAAATCGGA AGAAGATATC 
TTAAAGCCAA AATTCCAGGC CTTCAAGGGA ATAGGCTGTC TCTATGCAAA GGAGTCAGTA 
GACAAAACTT TGAAAGAGAA TATTGCCTTT AATACTGGCG GTGGGCATCA CAGTTCCTTA 
AAACATGAAG ATCACAATAG AAGTTTGATG GAAAATGGAT CTAAGTGTGT TAATGGCTCA 
TCAAGCAAAC TGACTTCATG CCAACCTGCA AAGAAGGTGA ATGACCCGAA CCACATTCAG 
CCTCCAAAAA GACAGTGTAC TTTTGAGAAC CAGAACCATA AAGTAATGTC CTCTACTAGT 
TGTGACAAGG GAAGTACAAA TCCTCTCCCA GGTCCGTTGC CATTGAAAGC TAAAACATCC 
CTGCATCTTA GTGCCAACAG TCACAAGGTA GACTCTGATG GAGACGCTTG CCATTGGCCT 
GAAAGTAGAA AAGCTTTAGA GAAAGAGAGG ACCAATTTAA GCAACCTCGA ATCTCTTGAG 
AAATCATCAA AGGTGTCTCC CAGGGAAGAC CCTCAGAAAA GCCCAGCTGG TTTCTCACTT 
TCTGATAGTA ATGCATCCTG TATAAATGCT AAGGATAAAC AAGCTGAAGA CAACCAGAAG 
CGATTGGCCG CCTTGGCAGC ATGGCAGAAA GCCAGGGAAG TACAGAAAAA ACTGGTGCAT 
AGTGCCCTGG CAAATTTGGA TGGTCATCCA GAGGACAAAA AAACACACAT TGTCTTTGCT 
TCTGATAATG AAAGTGAAAC GGAAGAGACA TCCACTCAGG AGCAAAGCTG TCCAGAAAAG 
GAGCTGATGA AAGAATCTGT GAGTAAATCG CCTGGGAAGC TGTTTGACAG CAGTGATGAT 
GAAGACTCAG ATTCCAAGGA GGACAGCACC AGGTTCAGCA TTAAACCTCA GTTTGAGGGC 
AGAGCTGGCC AGAAGCTTAT GGATCTGCAG TCTCAGTTTG GAAGTGATGA GAGATTCCGC 
ATGGACTCTA GATTTTTAGA GAGTGACAGT GAGGACGAAA AGAAAGAGCT AAATGAAGAT 
AAAGTGAATG AAGACGAGCT TGCTGCAGAA AAAAAGAAAA CCCTGAATGT TGTGCAAAGT 
GTTTTGAACA TCAATGTGAA CAACCCGACG AACAAAGGAT CAGTGGCTGC TAAGAAATTT 
AAGGATATCG TACATTATGA CCCAACAAAG CATGACCATG CCATTTATGA AAGAAAACAA 
GAAGATAAAG AAAAAGAAAG TAAGGCAACA CGGAAGAAGA AAAAGGAAGA AGCAGAGAAG 
CTGCCTGAGG TATCCCAAGA CATGTATTAT AACATTGCTG CAGATTTGAA AGAAATATTC 
CAAAGTATGA GCAACACAGA TGAAAAGGAA GAAGATGTGC CCAGGACTGA GGCTGGTGCT 
AGAGAGGGGA CTGGAAAAAT CAGGAACGCC GAGACACTGG CATGTGAGCC CGAGCAAACC 
ACTGGCTTTA CATTCTCTTT TTTTGATTCA GCCACTAAAG ATGAAAAGGA TGCCACCTAT 
AGAATCGAAC TAGTGAAACA TGGGAAGATA GTCTGTCCAA ATGATCCCCG ATTCCAAGAC 
AGTAGTTCAG AAGAAGAAGA TATTGCTGAA GAAGCAGATC ATAGCAAGCC AAGCCCTGGA 
GAAGCGGTTC CTGAGAATGA GGCCATTAGA TTTTTCTTTT TCTCTGAGAA TGACGACAGA 
CTTCGTGGTT CTAACTTATT CTGGAGTGGT ATGGGAGGTA GTATTAGCAG AAATTCTTGG 
GAAGCCCGAA CAAGTAGTCT CCTTTTGGAA TGTCGGAAGA AACATAAAGA AGCCAAAAGG 
AAGGTGAAAG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nol8 ( NM_001271397.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258326.1 Mus musculus nucleolar protein 8 (Nol8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06965
Clone ID Related Accession (Same CDS sequence)	XM_006516957.1 , XM_006516958.4 , XM_006516958.3 , NM_001271397.1
Accession Version	NM_001271397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	nucleolar protein 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK014994.1, BC125575.1 and AK147172.1. On Sep 20, 2012 this sequence version replaced NM_027607.1. Transcript Variant: This variant (1) encodes the protein. ##Evidence-Data-START## Transcript exon combination :: BC125575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGAC CTCTGGAAAA CGTTTCCAGC AATCTTGATA GATGCCCAAC ATCCATGCAA 
GGCAACAGAG AAATGAAGCG CCTCTTTGTG GGTGGACTTG GACAGGGCAT TTCTGAGACA 
GACCTACAAA ATCAGTTCGG CAGATTTGGA GAAGTTTCTG ACGTGGAGAT CATCACTCGG 
AAAGATGACC AAGGAAATTC ACAGAAAGTC TTTGCGTATG TTAACATTCA AATAACAGAA 
GCGGACCTGA AGAAATGTAT GTCTATTTTA AACAAAACAA AATGGAAAGG TGGAACACTA 
CAAATTCAGC TAGCAAAAGA AAGCTTTTTA CACAGATTGG CCCAAGAAAG AGAAGACGCA 
AAAGCAAAGA AAGAAAAATC AACAACAGGC AACCCCACCT TATTGGAAAA GATGGGAGCA 
GTGGATTTCC ATATGAAGGC TGTGCCTGGA ACAGAAGTAC CAGGGCATAA AAATTGGGTT 
GTGAGTAAGT TTGGAAGAGT CTTACCTGTT CTTCACCTTA AGAATCAACA AAAACATAAA 
ATTATGAAAT ATGATCCATC AAAATACTGC CACAATATAA AAAAGATTCC AGAGAATTTG 
ACAGAAACCA CTCCCATAGC TGAACTCACT TGGGAATTGG AAGGAGGCAA TGACCCTATG 
AGTAAGAAAC GTCGAGGAGA GTTCTCTGAC TTTCATATCC CTCCTCAAAA GGTCAAGAAA 
GTGCAGAAGA GCAATGATCC CATGGAGTCC AAGGTTTCTA ATATTGGTCT AAGGACTAAT 
CAGGTAATGG AGAAGAACAA ATCAACACAT CCTGTGACTG CACACGGGAC AGCCCCTAGT 
ACTGTTAATC CTTCTAAACA ACTCCTTGTG TCCAGTTCTG GTACTCAGAA ACCTAAACAT 
GTGGTTTTTC ATAATTCTGA CTTTGAAATT ATCTGGAATA AAAGTAGCAT GTCTGATGAT 
GATGTTGACT CGGAAGATGA ATTAAAAATG ATGATTGCAA AAGAGGAAAA CAGAGAGAAA 
CCTGGGCATT CCTCAGTCAA TGAGTCTGAA CATGATACTT TTGAAGTTGT TAGGGATGAT 
TTCAAATCAA ATATTCACAA GCTTTCTTCT TCAGTAAGCC TAGGAAATAA CCATGAATAT 
GATTCAAGTG ATACAGATGA AATTATTGCA ATGAAAACAA AAAATGCTAA GGTCAAAAAC 
AGTGCAGAAT CTTCACAACC AGAAAGGACT GTAAGCAAGA AAAGTTCTTT TCAAAAGATA 
GAACCTTCTA ATGACTGTAT AAAAGTACAA GGAATTAACA GCAACAAAGA ATCAGCCCTG 
TGTCATGGAG TAAAGTTTGT AAATCCCAAA TTTCCACCTG ACTCCAGTGG CAGTGACAGT 
GAAGAGTCCG AAGAAGATGA AGAATATAAA GTCTTGATGG AAAACTGTCC CCGTGTGAGT 
CTCACTTTGG CTGACTTGGA GCAGTTGGCT GGCAGTCATC GGAAGTTTCC AGGAAAAGAC 
AGTGAGACTA ATGGTCCACA AAATGACAGT CACTGCAAAT TTGACACAAC CTCCAAGAAT 
CCCAAGACTT CTGGTGACCT GTACAATGGC AGACAACAGT GCATTCTTCC TGAGGAGATT 
GTGGCCTCCC TTTTAGAGGA CGAGAATACT TACAGCAAAC AAAAATCGGA AGAAGATATC 
TTAAAGCCAA AATTCCAGGC CTTCAAGGGA ATAGGCTGTC TCTATGCAAA GGAGTCAGTA 
GACAAAACTT TGAAAGAGAA TATTGCCTTT AATACTGGCG GTGGGCATCA CAGTTCCTTA 
AAACATGAAG ATCACAATAG AAGTTTGATG GAAAATGGAT CTAAGTGTGT TAATGGCTCA 
TCAAGCAAAC TGACTTCATG CCAACCTGCA AAGAAGGTGA ATGACCCGAA CCACATTCAG 
CCTCCAAAAA GACAGTGTAC TTTTGAGAAC CAGAACCATA AAGTAATGTC CTCTACTAGT 
TGTGACAAGG GAAGTACAAA TCCTCTCCCA GGTCCGTTGC CATTGAAAGC TAAAACATCC 
CTGCATCTTA GTGCCAACAG TCACAAGGTA GACTCTGATG GAGACGCTTG CCATTGGCCT 
GAAAGTAGAA AAGCTTTAGA GAAAGAGAGG ACCAATTTAA GCAACCTCGA ATCTCTTGAG 
AAATCATCAA AGGTGTCTCC CAGGGAAGAC CCTCAGAAAA GCCCAGCTGG TTTCTCACTT 
TCTGATAGTA ATGCATCCTG TATAAATGCT AAGGATAAAC AAGCTGAAGA CAACCAGAAG 
CGATTGGCCG CCTTGGCAGC ATGGCAGAAA GCCAGGGAAG TACAGAAAAA ACTGGTGCAT 
AGTGCCCTGG CAAATTTGGA TGGTCATCCA GAGGACAAAA AAACACACAT TGTCTTTGCT 
TCTGATAATG AAAGTGAAAC GGAAGAGACA TCCACTCAGG AGCAAAGCTG TCCAGAAAAG 
GAGCTGATGA AAGAATCTGT GAGTAAATCG CCTGGGAAGC TGTTTGACAG CAGTGATGAT 
GAAGACTCAG ATTCCAAGGA GGACAGCACC AGGTTCAGCA TTAAACCTCA GTTTGAGGGC 
AGAGCTGGCC AGAAGCTTAT GGATCTGCAG TCTCAGTTTG GAAGTGATGA GAGATTCCGC 
ATGGACTCTA GATTTTTAGA GAGTGACAGT GAGGACGAAA AGAAAGAGCT AAATGAAGAT 
AAAGTGAATG AAGACGAGCT TGCTGCAGAA AAAAAGAAAA CCCTGAATGT TGTGCAAAGT 
GTTTTGAACA TCAATGTGAA CAACCCGACG AACAAAGGAT CAGTGGCTGC TAAGAAATTT 
AAGGATATCG TACATTATGA CCCAACAAAG CATGACCATG CCATTTATGA AAGAAAACAA 
GAAGATAAAG AAAAAGAAAG TAAGGCAACA CGGAAGAAGA AAAAGGAAGA AGCAGAGAAG 
CTGCCTGAGG TATCCCAAGA CATGTATTAT AACATTGCTG CAGATTTGAA AGAAATATTC 
CAAAGTATGA GCAACACAGA TGAAAAGGAA GAAGATGTGC CCAGGACTGA GGCTGGTGCT 
AGAGAGGGGA CTGGAAAAAT CAGGAACGCC GAGACACTGG CATGTGAGCC CGAGCAAACC 
ACTGGCTTTA CATTCTCTTT TTTTGATTCA GCCACTAAAG ATGAAAAGGA TGCCACCTAT 
AGAATCGAAC TAGTGAAACA TGGGAAGATA GTCTGTCCAA ATGATCCCCG ATTCCAAGAC 
AGTAGTTCAG AAGAAGAAGA TATTGCTGAA GAAGCAGATC ATAGCAAGCC AAGCCCTGGA 
GAAGCGGTTC CTGAGAATGA GGCCATTAGA TTTTTCTTTT TCTCTGAGAA TGACGACAGA 
CTTCGTGGTT CTAACTTATT CTGGAGTGGT ATGGGAGGTA GTATTAGCAG AAATTCTTGG 
GAAGCCCGAA CAAGTAGTCT CCTTTTGGAA TGTCGGAAGA AACATAAAGA AGCCAAAAGG 
AAGGTGAAAG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258326.1
CDS	569..4066
Translation	MERPLENVSSNLDRCPTSMQGNREMKRLFVGGLGQGISETDLQNQFGRFGEVSDVEIITRKDDQGNSQKVFAYVNIQITEADLKKCMSILNKTKWKGGTLQIQLAKESFLHRLAQEREDAKAKKEKSTTGNPTLLEKMGAVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQQKHKIMKYDPSKYCHNIKKIPENLTETTPIAELTWELEGGNDPMSKKRRGEFSDFHIPPQKVKKVQKSNDPMESKVSNIGLRTNQVMEKNKSTHPVTAHGTAPSTVNPSKQLLVSSSGTQKPKHVVFHNSDFEIIWNKSSMSDDDVDSEDELKMMIAKEENREKPGHSSVNESEHDTFEVVRDDFKSNIHKLSSSVSLGNNHEYDSSDTDEIIAMKTKNAKVKNSAESSQPERTVSKKSSFQKIEPSNDCIKVQGINSNKESALCHGVKFVNPKFPPDSSGSDSEESEEDEEYKVLMENCPRVSLTLADLEQLAGSHRKFPGKDSETNGPQNDSHCKFDTTSKNPKTSGDLYNGRQQCILPEEIVASLLEDENTYSKQKSEEDILKPKFQAFKGIGCLYAKESVDKTLKENIAFNTGGGHHSSLKHEDHNRSLMENGSKCVNGSSSKLTSCQPAKKVNDPNHIQPPKRQCTFENQNHKVMSSTSCDKGSTNPLPGPLPLKAKTSLHLSANSHKVDSDGDACHWPESRKALEKERTNLSNLESLEKSSKVSPREDPQKSPAGFSLSDSNASCINAKDKQAEDNQKRLAALAAWQKAREVQKKLVHSALANLDGHPEDKKTHIVFASDNESETEETSTQEQSCPEKELMKESVSKSPGKLFDSSDDEDSDSKEDSTRFSIKPQFEGRAGQKLMDLQSQFGSDERFRMDSRFLESDSEDEKKELNEDKVNEDELAAEKKKTLNVVQSVLNINVNNPTNKGSVAAKKFKDIVHYDPTKHDHAIYERKQEDKEKESKATRKKKKEEAEKLPEVSQDMYYNIAADLKEIFQSMSNTDEKEEDVPRTEAGAREGTGKIRNAETLACEPEQTTGFTFSFFDSATKDEKDATYRIELVKHGKIVCPNDPRFQDSSSEEEDIAEEADHSKPSPGEAVPENEAIRFFFFSENDDRLRGSNLFWSGMGGSISRNSWEARTSSLLLECRKKHKEAKRKVKAN

Target ORF information:

RefSeq Version	NM_001271397.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleolar protein 8 (Nol8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271397.1

ORF Insert Sequence:

ATGGAGCGAC CTCTGGAAAA CGTTTCCAGC AATCTTGATA GATGCCCAAC ATCCATGCAA 
GGCAACAGAG AAATGAAGCG CCTCTTTGTG GGTGGACTTG GACAGGGCAT TTCTGAGACA 
GACCTACAAA ATCAGTTCGG CAGATTTGGA GAAGTTTCTG ACGTGGAGAT CATCACTCGG 
AAAGATGACC AAGGAAATTC ACAGAAAGTC TTTGCGTATG TTAACATTCA AATAACAGAA 
GCGGACCTGA AGAAATGTAT GTCTATTTTA AACAAAACAA AATGGAAAGG TGGAACACTA 
CAAATTCAGC TAGCAAAAGA AAGCTTTTTA CACAGATTGG CCCAAGAAAG AGAAGACGCA 
AAAGCAAAGA AAGAAAAATC AACAACAGGC AACCCCACCT TATTGGAAAA GATGGGAGCA 
GTGGATTTCC ATATGAAGGC TGTGCCTGGA ACAGAAGTAC CAGGGCATAA AAATTGGGTT 
GTGAGTAAGT TTGGAAGAGT CTTACCTGTT CTTCACCTTA AGAATCAACA AAAACATAAA 
ATTATGAAAT ATGATCCATC AAAATACTGC CACAATATAA AAAAGATTCC AGAGAATTTG 
ACAGAAACCA CTCCCATAGC TGAACTCACT TGGGAATTGG AAGGAGGCAA TGACCCTATG 
AGTAAGAAAC GTCGAGGAGA GTTCTCTGAC TTTCATATCC CTCCTCAAAA GGTCAAGAAA 
GTGCAGAAGA GCAATGATCC CATGGAGTCC AAGGTTTCTA ATATTGGTCT AAGGACTAAT 
CAGGTAATGG AGAAGAACAA ATCAACACAT CCTGTGACTG CACACGGGAC AGCCCCTAGT 
ACTGTTAATC CTTCTAAACA ACTCCTTGTG TCCAGTTCTG GTACTCAGAA ACCTAAACAT 
GTGGTTTTTC ATAATTCTGA CTTTGAAATT ATCTGGAATA AAAGTAGCAT GTCTGATGAT 
GATGTTGACT CGGAAGATGA ATTAAAAATG ATGATTGCAA AAGAGGAAAA CAGAGAGAAA 
CCTGGGCATT CCTCAGTCAA TGAGTCTGAA CATGATACTT TTGAAGTTGT TAGGGATGAT 
TTCAAATCAA ATATTCACAA GCTTTCTTCT TCAGTAAGCC TAGGAAATAA CCATGAATAT 
GATTCAAGTG ATACAGATGA AATTATTGCA ATGAAAACAA AAAATGCTAA GGTCAAAAAC 
AGTGCAGAAT CTTCACAACC AGAAAGGACT GTAAGCAAGA AAAGTTCTTT TCAAAAGATA 
GAACCTTCTA ATGACTGTAT AAAAGTACAA GGAATTAACA GCAACAAAGA ATCAGCCCTG 
TGTCATGGAG TAAAGTTTGT AAATCCCAAA TTTCCACCTG ACTCCAGTGG CAGTGACAGT 
GAAGAGTCCG AAGAAGATGA AGAATATAAA GTCTTGATGG AAAACTGTCC CCGTGTGAGT 
CTCACTTTGG CTGACTTGGA GCAGTTGGCT GGCAGTCATC GGAAGTTTCC AGGAAAAGAC 
AGTGAGACTA ATGGTCCACA AAATGACAGT CACTGCAAAT TTGACACAAC CTCCAAGAAT 
CCCAAGACTT CTGGTGACCT GTACAATGGC AGACAACAGT GCATTCTTCC TGAGGAGATT 
GTGGCCTCCC TTTTAGAGGA CGAGAATACT TACAGCAAAC AAAAATCGGA AGAAGATATC 
TTAAAGCCAA AATTCCAGGC CTTCAAGGGA ATAGGCTGTC TCTATGCAAA GGAGTCAGTA 
GACAAAACTT TGAAAGAGAA TATTGCCTTT AATACTGGCG GTGGGCATCA CAGTTCCTTA 
AAACATGAAG ATCACAATAG AAGTTTGATG GAAAATGGAT CTAAGTGTGT TAATGGCTCA 
TCAAGCAAAC TGACTTCATG CCAACCTGCA AAGAAGGTGA ATGACCCGAA CCACATTCAG 
CCTCCAAAAA GACAGTGTAC TTTTGAGAAC CAGAACCATA AAGTAATGTC CTCTACTAGT 
TGTGACAAGG GAAGTACAAA TCCTCTCCCA GGTCCGTTGC CATTGAAAGC TAAAACATCC 
CTGCATCTTA GTGCCAACAG TCACAAGGTA GACTCTGATG GAGACGCTTG CCATTGGCCT 
GAAAGTAGAA AAGCTTTAGA GAAAGAGAGG ACCAATTTAA GCAACCTCGA ATCTCTTGAG 
AAATCATCAA AGGTGTCTCC CAGGGAAGAC CCTCAGAAAA GCCCAGCTGG TTTCTCACTT 
TCTGATAGTA ATGCATCCTG TATAAATGCT AAGGATAAAC AAGCTGAAGA CAACCAGAAG 
CGATTGGCCG CCTTGGCAGC ATGGCAGAAA GCCAGGGAAG TACAGAAAAA ACTGGTGCAT 
AGTGCCCTGG CAAATTTGGA TGGTCATCCA GAGGACAAAA AAACACACAT TGTCTTTGCT 
TCTGATAATG AAAGTGAAAC GGAAGAGACA TCCACTCAGG AGCAAAGCTG TCCAGAAAAG 
GAGCTGATGA AAGAATCTGT GAGTAAATCG CCTGGGAAGC TGTTTGACAG CAGTGATGAT 
GAAGACTCAG ATTCCAAGGA GGACAGCACC AGGTTCAGCA TTAAACCTCA GTTTGAGGGC 
AGAGCTGGCC AGAAGCTTAT GGATCTGCAG TCTCAGTTTG GAAGTGATGA GAGATTCCGC 
ATGGACTCTA GATTTTTAGA GAGTGACAGT GAGGACGAAA AGAAAGAGCT AAATGAAGAT 
AAAGTGAATG AAGACGAGCT TGCTGCAGAA AAAAAGAAAA CCCTGAATGT TGTGCAAAGT 
GTTTTGAACA TCAATGTGAA CAACCCGACG AACAAAGGAT CAGTGGCTGC TAAGAAATTT 
AAGGATATCG TACATTATGA CCCAACAAAG CATGACCATG CCATTTATGA AAGAAAACAA 
GAAGATAAAG AAAAAGAAAG TAAGGCAACA CGGAAGAAGA AAAAGGAAGA AGCAGAGAAG 
CTGCCTGAGG TATCCCAAGA CATGTATTAT AACATTGCTG CAGATTTGAA AGAAATATTC 
CAAAGTATGA GCAACACAGA TGAAAAGGAA GAAGATGTGC CCAGGACTGA GGCTGGTGCT 
AGAGAGGGGA CTGGAAAAAT CAGGAACGCC GAGACACTGG CATGTGAGCC CGAGCAAACC 
ACTGGCTTTA CATTCTCTTT TTTTGATTCA GCCACTAAAG ATGAAAAGGA TGCCACCTAT 
AGAATCGAAC TAGTGAAACA TGGGAAGATA GTCTGTCCAA ATGATCCCCG ATTCCAAGAC 
AGTAGTTCAG AAGAAGAAGA TATTGCTGAA GAAGCAGATC ATAGCAAGCC AAGCCCTGGA 
GAAGCGGTTC CTGAGAATGA GGCCATTAGA TTTTTCTTTT TCTCTGAGAA TGACGACAGA 
CTTCGTGGTT CTAACTTATT CTGGAGTGGT ATGGGAGGTA GTATTAGCAG AAATTCTTGG 
GAAGCCCGAA CAAGTAGTCT CCTTTTGGAA TGTCGGAAGA AACATAAAGA AGCCAAAAGG 
AAGGTGAAAG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nol8 ( XM_006516957.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517020.1 Mus musculus nucleolar protein 8 (Nol8), transcript variant X2, mRNA.

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CloneID	OMu06965
Clone ID Related Accession (Same CDS sequence)	XM_006516957.1 , XM_006516958.4 , XM_006516958.3 , NM_001271397.1
Accession Version	XM_006516957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nucleolar protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAC CTCTGGAAAA CGTTTCCAGC AATCTTGATA GATGCCCAAC ATCCATGCAA 
GGCAACAGAG AAATGAAGCG CCTCTTTGTG GGTGGACTTG GACAGGGCAT TTCTGAGACA 
GACCTACAAA ATCAGTTCGG CAGATTTGGA GAAGTTTCTG ACGTGGAGAT CATCACTCGG 
AAAGATGACC AAGGAAATTC ACAGAAAGTC TTTGCGTATG TTAACATTCA AATAACAGAA 
GCGGACCTGA AGAAATGTAT GTCTATTTTA AACAAAACAA AATGGAAAGG TGGAACACTA 
CAAATTCAGC TAGCAAAAGA AAGCTTTTTA CACAGATTGG CCCAAGAAAG AGAAGACGCA 
AAAGCAAAGA AAGAAAAATC AACAACAGGC AACCCCACCT TATTGGAAAA GATGGGAGCA 
GTGGATTTCC ATATGAAGGC TGTGCCTGGA ACAGAAGTAC CAGGGCATAA AAATTGGGTT 
GTGAGTAAGT TTGGAAGAGT CTTACCTGTT CTTCACCTTA AGAATCAACA AAAACATAAA 
ATTATGAAAT ATGATCCATC AAAATACTGC CACAATATAA AAAAGATTCC AGAGAATTTG 
ACAGAAACCA CTCCCATAGC TGAACTCACT TGGGAATTGG AAGGAGGCAA TGACCCTATG 
AGTAAGAAAC GTCGAGGAGA GTTCTCTGAC TTTCATATCC CTCCTCAAAA GGTCAAGAAA 
GTGCAGAAGA GCAATGATCC CATGGAGTCC AAGGTTTCTA ATATTGGTCT AAGGACTAAT 
CAGGTAATGG AGAAGAACAA ATCAACACAT CCTGTGACTG CACACGGGAC AGCCCCTAGT 
ACTGTTAATC CTTCTAAACA ACTCCTTGTG TCCAGTTCTG GTACTCAGAA ACCTAAACAT 
GTGGTTTTTC ATAATTCTGA CTTTGAAATT ATCTGGAATA AAAGTAGCAT GTCTGATGAT 
GATGTTGACT CGGAAGATGA ATTAAAAATG ATGATTGCAA AAGAGGAAAA CAGAGAGAAA 
CCTGGGCATT CCTCAGTCAA TGAGTCTGAA CATGATACTT TTGAAGTTGT TAGGGATGAT 
TTCAAATCAA ATATTCACAA GCTTTCTTCT TCAGTAAGCC TAGGAAATAA CCATGAATAT 
GATTCAAGTG ATACAGATGA AATTATTGCA ATGAAAACAA AAAATGCTAA GGTCAAAAAC 
AGTGCAGAAT CTTCACAACC AGAAAGGACT GTAAGCAAGA AAAGTTCTTT TCAAAAGATA 
GAACCTTCTA ATGACTGTAT AAAAGTACAA GGAATTAACA GCAACAAAGA ATCAGCCCTG 
TGTCATGGAG TAAAGTTTGT AAATCCCAAA TTTCCACCTG ACTCCAGTGG CAGTGACAGT 
GAAGAGTCCG AAGAAGATGA AGAATATAAA GTCTTGATGG AAAACTGTCC CCGTGTGAGT 
CTCACTTTGG CTGACTTGGA GCAGTTGGCT GGCAGTCATC GGAAGTTTCC AGGAAAAGAC 
AGTGAGACTA ATGGTCCACA AAATGACAGT CACTGCAAAT TTGACACAAC CTCCAAGAAT 
CCCAAGACTT CTGGTGACCT GTACAATGGC AGACAACAGT GCATTCTTCC TGAGGAGATT 
GTGGCCTCCC TTTTAGAGGA CGAGAATACT TACAGCAAAC AAAAATCGGA AGAAGATATC 
TTAAAGCCAA AATTCCAGGC CTTCAAGGGA ATAGGCTGTC TCTATGCAAA GGAGTCAGTA 
GACAAAACTT TGAAAGAGAA TATTGCCTTT AATACTGGCG GTGGGCATCA CAGTTCCTTA 
AAACATGAAG ATCACAATAG AAGTTTGATG GAAAATGGAT CTAAGTGTGT TAATGGCTCA 
TCAAGCAAAC TGACTTCATG CCAACCTGCA AAGAAGGTGA ATGACCCGAA CCACATTCAG 
CCTCCAAAAA GACAGTGTAC TTTTGAGAAC CAGAACCATA AAGTAATGTC CTCTACTAGT 
TGTGACAAGG GAAGTACAAA TCCTCTCCCA GGTCCGTTGC CATTGAAAGC TAAAACATCC 
CTGCATCTTA GTGCCAACAG TCACAAGGTA GACTCTGATG GAGACGCTTG CCATTGGCCT 
GAAAGTAGAA AAGCTTTAGA GAAAGAGAGG ACCAATTTAA GCAACCTCGA ATCTCTTGAG 
AAATCATCAA AGGTGTCTCC CAGGGAAGAC CCTCAGAAAA GCCCAGCTGG TTTCTCACTT 
TCTGATAGTA ATGCATCCTG TATAAATGCT AAGGATAAAC AAGCTGAAGA CAACCAGAAG 
CGATTGGCCG CCTTGGCAGC ATGGCAGAAA GCCAGGGAAG TACAGAAAAA ACTGGTGCAT 
AGTGCCCTGG CAAATTTGGA TGGTCATCCA GAGGACAAAA AAACACACAT TGTCTTTGCT 
TCTGATAATG AAAGTGAAAC GGAAGAGACA TCCACTCAGG AGCAAAGCTG TCCAGAAAAG 
GAGCTGATGA AAGAATCTGT GAGTAAATCG CCTGGGAAGC TGTTTGACAG CAGTGATGAT 
GAAGACTCAG ATTCCAAGGA GGACAGCACC AGGTTCAGCA TTAAACCTCA GTTTGAGGGC 
AGAGCTGGCC AGAAGCTTAT GGATCTGCAG TCTCAGTTTG GAAGTGATGA GAGATTCCGC 
ATGGACTCTA GATTTTTAGA GAGTGACAGT GAGGACGAAA AGAAAGAGCT AAATGAAGAT 
AAAGTGAATG AAGACGAGCT TGCTGCAGAA AAAAAGAAAA CCCTGAATGT TGTGCAAAGT 
GTTTTGAACA TCAATGTGAA CAACCCGACG AACAAAGGAT CAGTGGCTGC TAAGAAATTT 
AAGGATATCG TACATTATGA CCCAACAAAG CATGACCATG CCATTTATGA AAGAAAACAA 
GAAGATAAAG AAAAAGAAAG TAAGGCAACA CGGAAGAAGA AAAAGGAAGA AGCAGAGAAG 
CTGCCTGAGG TATCCCAAGA CATGTATTAT AACATTGCTG CAGATTTGAA AGAAATATTC 
CAAAGTATGA GCAACACAGA TGAAAAGGAA GAAGATGTGC CCAGGACTGA GGCTGGTGCT 
AGAGAGGGGA CTGGAAAAAT CAGGAACGCC GAGACACTGG CATGTGAGCC CGAGCAAACC 
ACTGGCTTTA CATTCTCTTT TTTTGATTCA GCCACTAAAG ATGAAAAGGA TGCCACCTAT 
AGAATCGAAC TAGTGAAACA TGGGAAGATA GTCTGTCCAA ATGATCCCCG ATTCCAAGAC 
AGTAGTTCAG AAGAAGAAGA TATTGCTGAA GAAGCAGATC ATAGCAAGCC AAGCCCTGGA 
GAAGCGGTTC CTGAGAATGA GGCCATTAGA TTTTTCTTTT TCTCTGAGAA TGACGACAGA 
CTTCGTGGTT CTAACTTATT CTGGAGTGGT ATGGGAGGTA GTATTAGCAG AAATTCTTGG 
GAAGCCCGAA CAAGTAGTCT CCTTTTGGAA TGTCGGAAGA AACATAAAGA AGCCAAAAGG 
AAGGTGAAAG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517020.1
CDS	378..3875
Translation	MERPLENVSSNLDRCPTSMQGNREMKRLFVGGLGQGISETDLQNQFGRFGEVSDVEIITRKDDQGNSQKVFAYVNIQITEADLKKCMSILNKTKWKGGTLQIQLAKESFLHRLAQEREDAKAKKEKSTTGNPTLLEKMGAVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQQKHKIMKYDPSKYCHNIKKIPENLTETTPIAELTWELEGGNDPMSKKRRGEFSDFHIPPQKVKKVQKSNDPMESKVSNIGLRTNQVMEKNKSTHPVTAHGTAPSTVNPSKQLLVSSSGTQKPKHVVFHNSDFEIIWNKSSMSDDDVDSEDELKMMIAKEENREKPGHSSVNESEHDTFEVVRDDFKSNIHKLSSSVSLGNNHEYDSSDTDEIIAMKTKNAKVKNSAESSQPERTVSKKSSFQKIEPSNDCIKVQGINSNKESALCHGVKFVNPKFPPDSSGSDSEESEEDEEYKVLMENCPRVSLTLADLEQLAGSHRKFPGKDSETNGPQNDSHCKFDTTSKNPKTSGDLYNGRQQCILPEEIVASLLEDENTYSKQKSEEDILKPKFQAFKGIGCLYAKESVDKTLKENIAFNTGGGHHSSLKHEDHNRSLMENGSKCVNGSSSKLTSCQPAKKVNDPNHIQPPKRQCTFENQNHKVMSSTSCDKGSTNPLPGPLPLKAKTSLHLSANSHKVDSDGDACHWPESRKALEKERTNLSNLESLEKSSKVSPREDPQKSPAGFSLSDSNASCINAKDKQAEDNQKRLAALAAWQKAREVQKKLVHSALANLDGHPEDKKTHIVFASDNESETEETSTQEQSCPEKELMKESVSKSPGKLFDSSDDEDSDSKEDSTRFSIKPQFEGRAGQKLMDLQSQFGSDERFRMDSRFLESDSEDEKKELNEDKVNEDELAAEKKKTLNVVQSVLNINVNNPTNKGSVAAKKFKDIVHYDPTKHDHAIYERKQEDKEKESKATRKKKKEEAEKLPEVSQDMYYNIAADLKEIFQSMSNTDEKEEDVPRTEAGAREGTGKIRNAETLACEPEQTTGFTFSFFDSATKDEKDATYRIELVKHGKIVCPNDPRFQDSSSEEEDIAEEADHSKPSPGEAVPENEAIRFFFFSENDDRLRGSNLFWSGMGGSISRNSWEARTSSLLLECRKKHKEAKRKVKAN

Target ORF information:

RefSeq Version	XM_006516957.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleolar protein 8 (Nol8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516957.1

ORF Insert Sequence:

ATGGAGCGAC CTCTGGAAAA CGTTTCCAGC AATCTTGATA GATGCCCAAC ATCCATGCAA 
GGCAACAGAG AAATGAAGCG CCTCTTTGTG GGTGGACTTG GACAGGGCAT TTCTGAGACA 
GACCTACAAA ATCAGTTCGG CAGATTTGGA GAAGTTTCTG ACGTGGAGAT CATCACTCGG 
AAAGATGACC AAGGAAATTC ACAGAAAGTC TTTGCGTATG TTAACATTCA AATAACAGAA 
GCGGACCTGA AGAAATGTAT GTCTATTTTA AACAAAACAA AATGGAAAGG TGGAACACTA 
CAAATTCAGC TAGCAAAAGA AAGCTTTTTA CACAGATTGG CCCAAGAAAG AGAAGACGCA 
AAAGCAAAGA AAGAAAAATC AACAACAGGC AACCCCACCT TATTGGAAAA GATGGGAGCA 
GTGGATTTCC ATATGAAGGC TGTGCCTGGA ACAGAAGTAC CAGGGCATAA AAATTGGGTT 
GTGAGTAAGT TTGGAAGAGT CTTACCTGTT CTTCACCTTA AGAATCAACA AAAACATAAA 
ATTATGAAAT ATGATCCATC AAAATACTGC CACAATATAA AAAAGATTCC AGAGAATTTG 
ACAGAAACCA CTCCCATAGC TGAACTCACT TGGGAATTGG AAGGAGGCAA TGACCCTATG 
AGTAAGAAAC GTCGAGGAGA GTTCTCTGAC TTTCATATCC CTCCTCAAAA GGTCAAGAAA 
GTGCAGAAGA GCAATGATCC CATGGAGTCC AAGGTTTCTA ATATTGGTCT AAGGACTAAT 
CAGGTAATGG AGAAGAACAA ATCAACACAT CCTGTGACTG CACACGGGAC AGCCCCTAGT 
ACTGTTAATC CTTCTAAACA ACTCCTTGTG TCCAGTTCTG GTACTCAGAA ACCTAAACAT 
GTGGTTTTTC ATAATTCTGA CTTTGAAATT ATCTGGAATA AAAGTAGCAT GTCTGATGAT 
GATGTTGACT CGGAAGATGA ATTAAAAATG ATGATTGCAA AAGAGGAAAA CAGAGAGAAA 
CCTGGGCATT CCTCAGTCAA TGAGTCTGAA CATGATACTT TTGAAGTTGT TAGGGATGAT 
TTCAAATCAA ATATTCACAA GCTTTCTTCT TCAGTAAGCC TAGGAAATAA CCATGAATAT 
GATTCAAGTG ATACAGATGA AATTATTGCA ATGAAAACAA AAAATGCTAA GGTCAAAAAC 
AGTGCAGAAT CTTCACAACC AGAAAGGACT GTAAGCAAGA AAAGTTCTTT TCAAAAGATA 
GAACCTTCTA ATGACTGTAT AAAAGTACAA GGAATTAACA GCAACAAAGA ATCAGCCCTG 
TGTCATGGAG TAAAGTTTGT AAATCCCAAA TTTCCACCTG ACTCCAGTGG CAGTGACAGT 
GAAGAGTCCG AAGAAGATGA AGAATATAAA GTCTTGATGG AAAACTGTCC CCGTGTGAGT 
CTCACTTTGG CTGACTTGGA GCAGTTGGCT GGCAGTCATC GGAAGTTTCC AGGAAAAGAC 
AGTGAGACTA ATGGTCCACA AAATGACAGT CACTGCAAAT TTGACACAAC CTCCAAGAAT 
CCCAAGACTT CTGGTGACCT GTACAATGGC AGACAACAGT GCATTCTTCC TGAGGAGATT 
GTGGCCTCCC TTTTAGAGGA CGAGAATACT TACAGCAAAC AAAAATCGGA AGAAGATATC 
TTAAAGCCAA AATTCCAGGC CTTCAAGGGA ATAGGCTGTC TCTATGCAAA GGAGTCAGTA 
GACAAAACTT TGAAAGAGAA TATTGCCTTT AATACTGGCG GTGGGCATCA CAGTTCCTTA 
AAACATGAAG ATCACAATAG AAGTTTGATG GAAAATGGAT CTAAGTGTGT TAATGGCTCA 
TCAAGCAAAC TGACTTCATG CCAACCTGCA AAGAAGGTGA ATGACCCGAA CCACATTCAG 
CCTCCAAAAA GACAGTGTAC TTTTGAGAAC CAGAACCATA AAGTAATGTC CTCTACTAGT 
TGTGACAAGG GAAGTACAAA TCCTCTCCCA GGTCCGTTGC CATTGAAAGC TAAAACATCC 
CTGCATCTTA GTGCCAACAG TCACAAGGTA GACTCTGATG GAGACGCTTG CCATTGGCCT 
GAAAGTAGAA AAGCTTTAGA GAAAGAGAGG ACCAATTTAA GCAACCTCGA ATCTCTTGAG 
AAATCATCAA AGGTGTCTCC CAGGGAAGAC CCTCAGAAAA GCCCAGCTGG TTTCTCACTT 
TCTGATAGTA ATGCATCCTG TATAAATGCT AAGGATAAAC AAGCTGAAGA CAACCAGAAG 
CGATTGGCCG CCTTGGCAGC ATGGCAGAAA GCCAGGGAAG TACAGAAAAA ACTGGTGCAT 
AGTGCCCTGG CAAATTTGGA TGGTCATCCA GAGGACAAAA AAACACACAT TGTCTTTGCT 
TCTGATAATG AAAGTGAAAC GGAAGAGACA TCCACTCAGG AGCAAAGCTG TCCAGAAAAG 
GAGCTGATGA AAGAATCTGT GAGTAAATCG CCTGGGAAGC TGTTTGACAG CAGTGATGAT 
GAAGACTCAG ATTCCAAGGA GGACAGCACC AGGTTCAGCA TTAAACCTCA GTTTGAGGGC 
AGAGCTGGCC AGAAGCTTAT GGATCTGCAG TCTCAGTTTG GAAGTGATGA GAGATTCCGC 
ATGGACTCTA GATTTTTAGA GAGTGACAGT GAGGACGAAA AGAAAGAGCT AAATGAAGAT 
AAAGTGAATG AAGACGAGCT TGCTGCAGAA AAAAAGAAAA CCCTGAATGT TGTGCAAAGT 
GTTTTGAACA TCAATGTGAA CAACCCGACG AACAAAGGAT CAGTGGCTGC TAAGAAATTT 
AAGGATATCG TACATTATGA CCCAACAAAG CATGACCATG CCATTTATGA AAGAAAACAA 
GAAGATAAAG AAAAAGAAAG TAAGGCAACA CGGAAGAAGA AAAAGGAAGA AGCAGAGAAG 
CTGCCTGAGG TATCCCAAGA CATGTATTAT AACATTGCTG CAGATTTGAA AGAAATATTC 
CAAAGTATGA GCAACACAGA TGAAAAGGAA GAAGATGTGC CCAGGACTGA GGCTGGTGCT 
AGAGAGGGGA CTGGAAAAAT CAGGAACGCC GAGACACTGG CATGTGAGCC CGAGCAAACC 
ACTGGCTTTA CATTCTCTTT TTTTGATTCA GCCACTAAAG ATGAAAAGGA TGCCACCTAT 
AGAATCGAAC TAGTGAAACA TGGGAAGATA GTCTGTCCAA ATGATCCCCG ATTCCAAGAC 
AGTAGTTCAG AAGAAGAAGA TATTGCTGAA GAAGCAGATC ATAGCAAGCC AAGCCCTGGA 
GAAGCGGTTC CTGAGAATGA GGCCATTAGA TTTTTCTTTT TCTCTGAGAA TGACGACAGA 
CTTCGTGGTT CTAACTTATT CTGGAGTGGT ATGGGAGGTA GTATTAGCAG AAATTCTTGG 
GAAGCCCGAA CAAGTAGTCT CCTTTTGGAA TGTCGGAAGA AACATAAAGA AGCCAAAAGG 
AAGGTGAAAG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nol8 ( XM_006516958.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517021.1 Mus musculus nucleolar protein 8 (Nol8), transcript variant X3, mRNA.

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CloneID	OMu06965
Clone ID Related Accession (Same CDS sequence)	XM_006516957.1 , XM_006516958.4 , XM_006516958.3 , NM_001271397.1
Accession Version	XM_006516958.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nucleolar protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516958.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAC CTCTGGAAAA CGTTTCCAGC AATCTTGATA GATGCCCAAC ATCCATGCAA 
GGCAACAGAG AAATGAAGCG CCTCTTTGTG GGTGGACTTG GACAGGGCAT TTCTGAGACA 
GACCTACAAA ATCAGTTCGG CAGATTTGGA GAAGTTTCTG ACGTGGAGAT CATCACTCGG 
AAAGATGACC AAGGAAATTC ACAGAAAGTC TTTGCGTATG TTAACATTCA AATAACAGAA 
GCGGACCTGA AGAAATGTAT GTCTATTTTA AACAAAACAA AATGGAAAGG TGGAACACTA 
CAAATTCAGC TAGCAAAAGA AAGCTTTTTA CACAGATTGG CCCAAGAAAG AGAAGACGCA 
AAAGCAAAGA AAGAAAAATC AACAACAGGC AACCCCACCT TATTGGAAAA GATGGGAGCA 
GTGGATTTCC ATATGAAGGC TGTGCCTGGA ACAGAAGTAC CAGGGCATAA AAATTGGGTT 
GTGAGTAAGT TTGGAAGAGT CTTACCTGTT CTTCACCTTA AGAATCAACA AAAACATAAA 
ATTATGAAAT ATGATCCATC AAAATACTGC CACAATATAA AAAAGATTCC AGAGAATTTG 
ACAGAAACCA CTCCCATAGC TGAACTCACT TGGGAATTGG AAGGAGGCAA TGACCCTATG 
AGTAAGAAAC GTCGAGGAGA GTTCTCTGAC TTTCATATCC CTCCTCAAAA GGTCAAGAAA 
GTGCAGAAGA GCAATGATCC CATGGAGTCC AAGGTTTCTA ATATTGGTCT AAGGACTAAT 
CAGGTAATGG AGAAGAACAA ATCAACACAT CCTGTGACTG CACACGGGAC AGCCCCTAGT 
ACTGTTAATC CTTCTAAACA ACTCCTTGTG TCCAGTTCTG GTACTCAGAA ACCTAAACAT 
GTGGTTTTTC ATAATTCTGA CTTTGAAATT ATCTGGAATA AAAGTAGCAT GTCTGATGAT 
GATGTTGACT CGGAAGATGA ATTAAAAATG ATGATTGCAA AAGAGGAAAA CAGAGAGAAA 
CCTGGGCATT CCTCAGTCAA TGAGTCTGAA CATGATACTT TTGAAGTTGT TAGGGATGAT 
TTCAAATCAA ATATTCACAA GCTTTCTTCT TCAGTAAGCC TAGGAAATAA CCATGAATAT 
GATTCAAGTG ATACAGATGA AATTATTGCA ATGAAAACAA AAAATGCTAA GGTCAAAAAC 
AGTGCAGAAT CTTCACAACC AGAAAGGACT GTAAGCAAGA AAAGTTCTTT TCAAAAGATA 
GAACCTTCTA ATGACTGTAT AAAAGTACAA GGAATTAACA GCAACAAAGA ATCAGCCCTG 
TGTCATGGAG TAAAGTTTGT AAATCCCAAA TTTCCACCTG ACTCCAGTGG CAGTGACAGT 
GAAGAGTCCG AAGAAGATGA AGAATATAAA GTCTTGATGG AAAACTGTCC CCGTGTGAGT 
CTCACTTTGG CTGACTTGGA GCAGTTGGCT GGCAGTCATC GGAAGTTTCC AGGAAAAGAC 
AGTGAGACTA ATGGTCCACA AAATGACAGT CACTGCAAAT TTGACACAAC CTCCAAGAAT 
CCCAAGACTT CTGGTGACCT GTACAATGGC AGACAACAGT GCATTCTTCC TGAGGAGATT 
GTGGCCTCCC TTTTAGAGGA CGAGAATACT TACAGCAAAC AAAAATCGGA AGAAGATATC 
TTAAAGCCAA AATTCCAGGC CTTCAAGGGA ATAGGCTGTC TCTATGCAAA GGAGTCAGTA 
GACAAAACTT TGAAAGAGAA TATTGCCTTT AATACTGGCG GTGGGCATCA CAGTTCCTTA 
AAACATGAAG ATCACAATAG AAGTTTGATG GAAAATGGAT CTAAGTGTGT TAATGGCTCA 
TCAAGCAAAC TGACTTCATG CCAACCTGCA AAGAAGGTGA ATGACCCGAA CCACATTCAG 
CCTCCAAAAA GACAGTGTAC TTTTGAGAAC CAGAACCATA AAGTAATGTC CTCTACTAGT 
TGTGACAAGG GAAGTACAAA TCCTCTCCCA GGTCCGTTGC CATTGAAAGC TAAAACATCC 
CTGCATCTTA GTGCCAACAG TCACAAGGTA GACTCTGATG GAGACGCTTG CCATTGGCCT 
GAAAGTAGAA AAGCTTTAGA GAAAGAGAGG ACCAATTTAA GCAACCTCGA ATCTCTTGAG 
AAATCATCAA AGGTGTCTCC CAGGGAAGAC CCTCAGAAAA GCCCAGCTGG TTTCTCACTT 
TCTGATAGTA ATGCATCCTG TATAAATGCT AAGGATAAAC AAGCTGAAGA CAACCAGAAG 
CGATTGGCCG CCTTGGCAGC ATGGCAGAAA GCCAGGGAAG TACAGAAAAA ACTGGTGCAT 
AGTGCCCTGG CAAATTTGGA TGGTCATCCA GAGGACAAAA AAACACACAT TGTCTTTGCT 
TCTGATAATG AAAGTGAAAC GGAAGAGACA TCCACTCAGG AGCAAAGCTG TCCAGAAAAG 
GAGCTGATGA AAGAATCTGT GAGTAAATCG CCTGGGAAGC TGTTTGACAG CAGTGATGAT 
GAAGACTCAG ATTCCAAGGA GGACAGCACC AGGTTCAGCA TTAAACCTCA GTTTGAGGGC 
AGAGCTGGCC AGAAGCTTAT GGATCTGCAG TCTCAGTTTG GAAGTGATGA GAGATTCCGC 
ATGGACTCTA GATTTTTAGA GAGTGACAGT GAGGACGAAA AGAAAGAGCT AAATGAAGAT 
AAAGTGAATG AAGACGAGCT TGCTGCAGAA AAAAAGAAAA CCCTGAATGT TGTGCAAAGT 
GTTTTGAACA TCAATGTGAA CAACCCGACG AACAAAGGAT CAGTGGCTGC TAAGAAATTT 
AAGGATATCG TACATTATGA CCCAACAAAG CATGACCATG CCATTTATGA AAGAAAACAA 
GAAGATAAAG AAAAAGAAAG TAAGGCAACA CGGAAGAAGA AAAAGGAAGA AGCAGAGAAG 
CTGCCTGAGG TATCCCAAGA CATGTATTAT AACATTGCTG CAGATTTGAA AGAAATATTC 
CAAAGTATGA GCAACACAGA TGAAAAGGAA GAAGATGTGC CCAGGACTGA GGCTGGTGCT 
AGAGAGGGGA CTGGAAAAAT CAGGAACGCC GAGACACTGG CATGTGAGCC CGAGCAAACC 
ACTGGCTTTA CATTCTCTTT TTTTGATTCA GCCACTAAAG ATGAAAAGGA TGCCACCTAT 
AGAATCGAAC TAGTGAAACA TGGGAAGATA GTCTGTCCAA ATGATCCCCG ATTCCAAGAC 
AGTAGTTCAG AAGAAGAAGA TATTGCTGAA GAAGCAGATC ATAGCAAGCC AAGCCCTGGA 
GAAGCGGTTC CTGAGAATGA GGCCATTAGA TTTTTCTTTT TCTCTGAGAA TGACGACAGA 
CTTCGTGGTT CTAACTTATT CTGGAGTGGT ATGGGAGGTA GTATTAGCAG AAATTCTTGG 
GAAGCCCGAA CAAGTAGTCT CCTTTTGGAA TGTCGGAAGA AACATAAAGA AGCCAAAAGG 
AAGGTGAAAG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517021.1
CDS	148..3645
Translation	MERPLENVSSNLDRCPTSMQGNREMKRLFVGGLGQGISETDLQNQFGRFGEVSDVEIITRKDDQGNSQKVFAYVNIQITEADLKKCMSILNKTKWKGGTLQIQLAKESFLHRLAQEREDAKAKKEKSTTGNPTLLEKMGAVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQQKHKIMKYDPSKYCHNIKKIPENLTETTPIAELTWELEGGNDPMSKKRRGEFSDFHIPPQKVKKVQKSNDPMESKVSNIGLRTNQVMEKNKSTHPVTAHGTAPSTVNPSKQLLVSSSGTQKPKHVVFHNSDFEIIWNKSSMSDDDVDSEDELKMMIAKEENREKPGHSSVNESEHDTFEVVRDDFKSNIHKLSSSVSLGNNHEYDSSDTDEIIAMKTKNAKVKNSAESSQPERTVSKKSSFQKIEPSNDCIKVQGINSNKESALCHGVKFVNPKFPPDSSGSDSEESEEDEEYKVLMENCPRVSLTLADLEQLAGSHRKFPGKDSETNGPQNDSHCKFDTTSKNPKTSGDLYNGRQQCILPEEIVASLLEDENTYSKQKSEEDILKPKFQAFKGIGCLYAKESVDKTLKENIAFNTGGGHHSSLKHEDHNRSLMENGSKCVNGSSSKLTSCQPAKKVNDPNHIQPPKRQCTFENQNHKVMSSTSCDKGSTNPLPGPLPLKAKTSLHLSANSHKVDSDGDACHWPESRKALEKERTNLSNLESLEKSSKVSPREDPQKSPAGFSLSDSNASCINAKDKQAEDNQKRLAALAAWQKAREVQKKLVHSALANLDGHPEDKKTHIVFASDNESETEETSTQEQSCPEKELMKESVSKSPGKLFDSSDDEDSDSKEDSTRFSIKPQFEGRAGQKLMDLQSQFGSDERFRMDSRFLESDSEDEKKELNEDKVNEDELAAEKKKTLNVVQSVLNINVNNPTNKGSVAAKKFKDIVHYDPTKHDHAIYERKQEDKEKESKATRKKKKEEAEKLPEVSQDMYYNIAADLKEIFQSMSNTDEKEEDVPRTEAGAREGTGKIRNAETLACEPEQTTGFTFSFFDSATKDEKDATYRIELVKHGKIVCPNDPRFQDSSSEEEDIAEEADHSKPSPGEAVPENEAIRFFFFSENDDRLRGSNLFWSGMGGSISRNSWEARTSSLLLECRKKHKEAKRKVKAN

Target ORF information:

RefSeq Version	XM_006516958.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleolar protein 8 (Nol8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516958.3

ORF Insert Sequence:

ATGGAGCGAC CTCTGGAAAA CGTTTCCAGC AATCTTGATA GATGCCCAAC ATCCATGCAA 
GGCAACAGAG AAATGAAGCG CCTCTTTGTG GGTGGACTTG GACAGGGCAT TTCTGAGACA 
GACCTACAAA ATCAGTTCGG CAGATTTGGA GAAGTTTCTG ACGTGGAGAT CATCACTCGG 
AAAGATGACC AAGGAAATTC ACAGAAAGTC TTTGCGTATG TTAACATTCA AATAACAGAA 
GCGGACCTGA AGAAATGTAT GTCTATTTTA AACAAAACAA AATGGAAAGG TGGAACACTA 
CAAATTCAGC TAGCAAAAGA AAGCTTTTTA CACAGATTGG CCCAAGAAAG AGAAGACGCA 
AAAGCAAAGA AAGAAAAATC AACAACAGGC AACCCCACCT TATTGGAAAA GATGGGAGCA 
GTGGATTTCC ATATGAAGGC TGTGCCTGGA ACAGAAGTAC CAGGGCATAA AAATTGGGTT 
GTGAGTAAGT TTGGAAGAGT CTTACCTGTT CTTCACCTTA AGAATCAACA AAAACATAAA 
ATTATGAAAT ATGATCCATC AAAATACTGC CACAATATAA AAAAGATTCC AGAGAATTTG 
ACAGAAACCA CTCCCATAGC TGAACTCACT TGGGAATTGG AAGGAGGCAA TGACCCTATG 
AGTAAGAAAC GTCGAGGAGA GTTCTCTGAC TTTCATATCC CTCCTCAAAA GGTCAAGAAA 
GTGCAGAAGA GCAATGATCC CATGGAGTCC AAGGTTTCTA ATATTGGTCT AAGGACTAAT 
CAGGTAATGG AGAAGAACAA ATCAACACAT CCTGTGACTG CACACGGGAC AGCCCCTAGT 
ACTGTTAATC CTTCTAAACA ACTCCTTGTG TCCAGTTCTG GTACTCAGAA ACCTAAACAT 
GTGGTTTTTC ATAATTCTGA CTTTGAAATT ATCTGGAATA AAAGTAGCAT GTCTGATGAT 
GATGTTGACT CGGAAGATGA ATTAAAAATG ATGATTGCAA AAGAGGAAAA CAGAGAGAAA 
CCTGGGCATT CCTCAGTCAA TGAGTCTGAA CATGATACTT TTGAAGTTGT TAGGGATGAT 
TTCAAATCAA ATATTCACAA GCTTTCTTCT TCAGTAAGCC TAGGAAATAA CCATGAATAT 
GATTCAAGTG ATACAGATGA AATTATTGCA ATGAAAACAA AAAATGCTAA GGTCAAAAAC 
AGTGCAGAAT CTTCACAACC AGAAAGGACT GTAAGCAAGA AAAGTTCTTT TCAAAAGATA 
GAACCTTCTA ATGACTGTAT AAAAGTACAA GGAATTAACA GCAACAAAGA ATCAGCCCTG 
TGTCATGGAG TAAAGTTTGT AAATCCCAAA TTTCCACCTG ACTCCAGTGG CAGTGACAGT 
GAAGAGTCCG AAGAAGATGA AGAATATAAA GTCTTGATGG AAAACTGTCC CCGTGTGAGT 
CTCACTTTGG CTGACTTGGA GCAGTTGGCT GGCAGTCATC GGAAGTTTCC AGGAAAAGAC 
AGTGAGACTA ATGGTCCACA AAATGACAGT CACTGCAAAT TTGACACAAC CTCCAAGAAT 
CCCAAGACTT CTGGTGACCT GTACAATGGC AGACAACAGT GCATTCTTCC TGAGGAGATT 
GTGGCCTCCC TTTTAGAGGA CGAGAATACT TACAGCAAAC AAAAATCGGA AGAAGATATC 
TTAAAGCCAA AATTCCAGGC CTTCAAGGGA ATAGGCTGTC TCTATGCAAA GGAGTCAGTA 
GACAAAACTT TGAAAGAGAA TATTGCCTTT AATACTGGCG GTGGGCATCA CAGTTCCTTA 
AAACATGAAG ATCACAATAG AAGTTTGATG GAAAATGGAT CTAAGTGTGT TAATGGCTCA 
TCAAGCAAAC TGACTTCATG CCAACCTGCA AAGAAGGTGA ATGACCCGAA CCACATTCAG 
CCTCCAAAAA GACAGTGTAC TTTTGAGAAC CAGAACCATA AAGTAATGTC CTCTACTAGT 
TGTGACAAGG GAAGTACAAA TCCTCTCCCA GGTCCGTTGC CATTGAAAGC TAAAACATCC 
CTGCATCTTA GTGCCAACAG TCACAAGGTA GACTCTGATG GAGACGCTTG CCATTGGCCT 
GAAAGTAGAA AAGCTTTAGA GAAAGAGAGG ACCAATTTAA GCAACCTCGA ATCTCTTGAG 
AAATCATCAA AGGTGTCTCC CAGGGAAGAC CCTCAGAAAA GCCCAGCTGG TTTCTCACTT 
TCTGATAGTA ATGCATCCTG TATAAATGCT AAGGATAAAC AAGCTGAAGA CAACCAGAAG 
CGATTGGCCG CCTTGGCAGC ATGGCAGAAA GCCAGGGAAG TACAGAAAAA ACTGGTGCAT 
AGTGCCCTGG CAAATTTGGA TGGTCATCCA GAGGACAAAA AAACACACAT TGTCTTTGCT 
TCTGATAATG AAAGTGAAAC GGAAGAGACA TCCACTCAGG AGCAAAGCTG TCCAGAAAAG 
GAGCTGATGA AAGAATCTGT GAGTAAATCG CCTGGGAAGC TGTTTGACAG CAGTGATGAT 
GAAGACTCAG ATTCCAAGGA GGACAGCACC AGGTTCAGCA TTAAACCTCA GTTTGAGGGC 
AGAGCTGGCC AGAAGCTTAT GGATCTGCAG TCTCAGTTTG GAAGTGATGA GAGATTCCGC 
ATGGACTCTA GATTTTTAGA GAGTGACAGT GAGGACGAAA AGAAAGAGCT AAATGAAGAT 
AAAGTGAATG AAGACGAGCT TGCTGCAGAA AAAAAGAAAA CCCTGAATGT TGTGCAAAGT 
GTTTTGAACA TCAATGTGAA CAACCCGACG AACAAAGGAT CAGTGGCTGC TAAGAAATTT 
AAGGATATCG TACATTATGA CCCAACAAAG CATGACCATG CCATTTATGA AAGAAAACAA 
GAAGATAAAG AAAAAGAAAG TAAGGCAACA CGGAAGAAGA AAAAGGAAGA AGCAGAGAAG 
CTGCCTGAGG TATCCCAAGA CATGTATTAT AACATTGCTG CAGATTTGAA AGAAATATTC 
CAAAGTATGA GCAACACAGA TGAAAAGGAA GAAGATGTGC CCAGGACTGA GGCTGGTGCT 
AGAGAGGGGA CTGGAAAAAT CAGGAACGCC GAGACACTGG CATGTGAGCC CGAGCAAACC 
ACTGGCTTTA CATTCTCTTT TTTTGATTCA GCCACTAAAG ATGAAAAGGA TGCCACCTAT 
AGAATCGAAC TAGTGAAACA TGGGAAGATA GTCTGTCCAA ATGATCCCCG ATTCCAAGAC 
AGTAGTTCAG AAGAAGAAGA TATTGCTGAA GAAGCAGATC ATAGCAAGCC AAGCCCTGGA 
GAAGCGGTTC CTGAGAATGA GGCCATTAGA TTTTTCTTTT TCTCTGAGAA TGACGACAGA 
CTTCGTGGTT CTAACTTATT CTGGAGTGGT ATGGGAGGTA GTATTAGCAG AAATTCTTGG 
GAAGCCCGAA CAAGTAGTCT CCTTTTGGAA TGTCGGAAGA AACATAAAGA AGCCAAAAGG 
AAGGTGAAAG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nol8 ( XM_006516956.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517019.2 Mus musculus nucleolar protein 8 (Nol8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70599
Clone ID Related Accession (Same CDS sequence)	XM_006516956.3
Accession Version	XM_006516956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nucleolar protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGCA ACAGAGAAAT GAAGCGCCTC TTTGTGGGTG GACTTGGACA GGGCATTTCT 
GAGACAGACC TACAAAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGACGT GGAGATCATC 
ACTCGGAAAG ATGACCAAGG AAATTCACAG AAAGTCTTTG CGTATGTTAA CATTCAAATA 
ACAGAAGCGG ACCTGAAGAA ATGTATGTCT ATTTTAAACA AAACAAAATG GAAAGGTGGA 
ACACTACAAA TTCAGCTAGC AAAAGAAAGC TTTTTACACA GATTGGCCCA AGAAAGAGAA 
GACGCAAAAG CAAAGAAAGA AAAATCAACA ACAGGCAACC CCACCTTATT GGAAAAGATG 
GGAGCAGTGG ATTTCCATAT GAAGGCTGTG CCTGGAACAG AAGTACCAGG GCATAAAAAT 
TGGGTTGTGA GTAAGTTTGG AAGAGTCTTA CCTGTTCTTC ACCTTAAGAA TCAACAAAAA 
CATAAAATTA TGAAATATGA TCCATCAAAA TACTGCCACA ATATAAAAAA GATTCCAGAG 
AATTTGACAG AAACCACTCC CATAGCTGAA CTCACTTGGG AATTGGAAGG AGGCAATGAC 
CCTATGAGTA AGAAACGTCG AGGAGAGTTC TCTGACTTTC ATATCCCTCC TCAAAAGGTC 
AAGAAAGTGC AGAAGAGCAA TGATCCCATG GAGTCCAAGG TTTCTAATAT TGGTCTAAGG 
ACTAATCAGG TAATGGAGAA GAACAAATCA ACACATCCTG TGACTGCACA CGGGACAGCC 
CCTAGTACTG TTAATCCTTC TAAACAACTC CTTGTGTCCA GTTCTGGTAC TCAGAAACCT 
AAACATGTGG TTTTTCATAA TTCTGACTTT GAAATTATCT GGAATAAAAG TAGCATGTCT 
GATGATGATG TTGACTCGGA AGATGAATTA AAAATGATGA TTGCAAAAGA GGAAAACAGA 
GAGAAACCTG GGCATTCCTC AGTCAATGAG TCTGAACATG ATACTTTTGA AGTTGTTAGG 
GATGATTTCA AATCAAATAT TCACAAGCTT TCTTCTTCAG TAAGCCTAGG AAATAACCAT 
GAATATGATT CAAGTGATAC AGATGAAATT ATTGCAATGA AAACAAAAAA TGCTAAGGTC 
AAAAACAGTG CAGAATCTTC ACAACCAGAA AGGACTGTAA GCAAGAAAAG TTCTTTTCAA 
AAGATAGAAC CTTCTAATGA CTGTATAAAA GTACAAGGAA TTAACAGCAA CAAAGAATCA 
GCCCTGTGTC ATGGAGTAAA GTTTGTAAAT CCCAAATTTC CACCTGACTC CAGTGGCAGT 
GACAGTGAAG AGTCCGAAGA AGATGAAGAA TATAAAGTCT TGATGGAAAA CTGTCCCCGT 
GTGAGTCTCA CTTTGGCTGA CTTGGAGCAG TTGGCTGGCA GTCATCGGAA GTTTCCAGGA 
AAAGACAGTG AGACTAATGG TCCACAAAAT GACAGTCACT GCAAATTTGA CACAACCTCC 
AAGAATCCCA AGACTTCTGG TGACCTGTAC AATGGCAGAC AACAGTGCAT TCTTCCTGAG 
GAGATTGTGG CCTCCCTTTT AGAGGACGAG AATACTTACA GCAAACAAAA ATCGGAAGAA 
GATATCTTAA AGCCAAAATT CCAGGCCTTC AAGGGAATAG GCTGTCTCTA TGCAAAGGAG 
TCAGTAGACA AAACTTTGAA AGAGAATATT GCCTTTAATA CTGGCGGTGG GCATCACAGT 
TCCTTAAAAC ATGAAGATCA CAATAGAAGT TTGATGGAAA ATGGATCTAA GTGTGTTAAT 
GGCTCATCAA GCAAACTGAC TTCATGCCAA CCTGCAAAGA AGGTGAATGA CCCGAACCAC 
ATTCAGCCTC CAAAAAGACA GTGTACTTTT GAGAACCAGA ACCATAAAGT AATGTCCTCT 
ACTAGTTGTG ACAAGGGAAG TACAAATCCT CTCCCAGGTC CGTTGCCATT GAAAGCTAAA 
ACATCCCTGC ATCTTAGTGC CAACAGTCAC AAGGTAGACT CTGATGGAGA CGCTTGCCAT 
TGGCCTGAAA GTAGAAAAGC TTTAGAGAAA GAGAGGACCA ATTTAAGCAA CCTCGAATCT 
CTTGAGAAAT CATCAAAGGT GTCTCCCAGG GAAGACCCTC AGAAAAGCCC AGCTGGTTTC 
TCACTTTCTG ATAGTAATGC ATCCTGTATA AATGCTAAGG ATAAACAAGC TGAAGACAAC 
CAGAAGCGAT TGGCCGCCTT GGCAGCATGG CAGAAAGCCA GGGAAGTACA GAAAAAACTG 
GTGCATAGTG CCCTGGCAAA TTTGGATGGT CATCCAGAGG ACAAAAAAAC ACACATTGTC 
TTTGCTTCTG ATAATGAAAG TGAAACGGAA GAGACATCCA CTCAGGAGCA AAGCTGTCCA 
GAAAAGGAGC TGATGAAAGA ATCTGTGAGT AAATCGCCTG GGAAGCTGTT TGACAGCAGT 
GATGATGAAG ACTCAGATTC CAAGGAGGAC AGCACCAGGT TCAGCATTAA ACCTCAGTTT 
GAGGGCAGAG CTGGCCAGAA GCTTATGGAT CTGCAGTCTC AGTTTGGAAG TGATGAGAGA 
TTCCGCATGG ACTCTAGATT TTTAGAGAGT GACAGTGAGG ACGAAAAGAA AGAGCTAAAT 
GAAGATAAAG TGAATGAAGA CGAGCTTGCT GCAGAAAAAA AGAAAACCCT GAATGTTGTG 
CAAAGTGTTT TGAACATCAA TGTGAACAAC CCGACGAACA AAGGATCAGT GGCTGCTAAG 
AAATTTAAGG ATATCGTACA TTATGACCCA ACAAAGCATG ACCATGCCAT TTATGAAAGA 
AAACAAGAAG ATAAAGAAAA AGAAAGTAAG GCAACACGGA AGAAGAAAAA GGAAGAAGCA 
GAGAAGCTGC CTGAGGTATC CCAAGACATG TATTATAACA TTGCTGCAGA TTTGAAAGAA 
ATATTCCAAA GTATGAGCAA CACAGATGAA AAGGAAGAAG ATGTGCCCAG GACTGAGGCT 
GGTGCTAGAG AGGGGACTGG AAAAATCAGG AACGCCGAGA CACTGGCATG TGAGCCCGAG 
CAAACCACTG GCTTTACATT CTCTTTTTTT GATTCAGCCA CTAAAGATGA AAAGGATGCC 
ACCTATAGAA TCGAACTAGT GAAACATGGG AAGATAGTCT GTCCAAATGA TCCCCGATTC 
CAAGACAGTA GTTCAGAAGA AGAAGATATT GCTGAAGAAG CAGATCATAG CAAGCCAAGC 
CCTGGAGAAG CGGTTCCTGA GAATGAGGCC ATTAGATTTT TCTTTTTCTC TGAGAATGAC 
GACAGACTTC GTGGTTCTAA CTTATTCTGG AGTGGTATGG GAGGTAGTAT TAGCAGAAAT 
TCTTGGGAAG CCCGAACAAG TAGTCTCCTT TTGGAATGTC GGAAGAAACA TAAAGAAGCC 
AAAAGGAAGG TGAAAGCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517019.2
CDS	65..3508
Translation	MQGNREMKRLFVGGLGQGISETDLQNQFGRFGEVSDVEIITRKDDQGNSQKVFAYVNIQITEADLKKCMSILNKTKWKGGTLQIQLAKESFLHRLAQEREDAKAKKEKSTTGNPTLLEKMGAVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQQKHKIMKYDPSKYCHNIKKIPENLTETTPIAELTWELEGGNDPMSKKRRGEFSDFHIPPQKVKKVQKSNDPMESKVSNIGLRTNQVMEKNKSTHPVTAHGTAPSTVNPSKQLLVSSSGTQKPKHVVFHNSDFEIIWNKSSMSDDDVDSEDELKMMIAKEENREKPGHSSVNESEHDTFEVVRDDFKSNIHKLSSSVSLGNNHEYDSSDTDEIIAMKTKNAKVKNSAESSQPERTVSKKSSFQKIEPSNDCIKVQGINSNKESALCHGVKFVNPKFPPDSSGSDSEESEEDEEYKVLMENCPRVSLTLADLEQLAGSHRKFPGKDSETNGPQNDSHCKFDTTSKNPKTSGDLYNGRQQCILPEEIVASLLEDENTYSKQKSEEDILKPKFQAFKGIGCLYAKESVDKTLKENIAFNTGGGHHSSLKHEDHNRSLMENGSKCVNGSSSKLTSCQPAKKVNDPNHIQPPKRQCTFENQNHKVMSSTSCDKGSTNPLPGPLPLKAKTSLHLSANSHKVDSDGDACHWPESRKALEKERTNLSNLESLEKSSKVSPREDPQKSPAGFSLSDSNASCINAKDKQAEDNQKRLAALAAWQKAREVQKKLVHSALANLDGHPEDKKTHIVFASDNESETEETSTQEQSCPEKELMKESVSKSPGKLFDSSDDEDSDSKEDSTRFSIKPQFEGRAGQKLMDLQSQFGSDERFRMDSRFLESDSEDEKKELNEDKVNEDELAAEKKKTLNVVQSVLNINVNNPTNKGSVAAKKFKDIVHYDPTKHDHAIYERKQEDKEKESKATRKKKKEEAEKLPEVSQDMYYNIAADLKEIFQSMSNTDEKEEDVPRTEAGAREGTGKIRNAETLACEPEQTTGFTFSFFDSATKDEKDATYRIELVKHGKIVCPNDPRFQDSSSEEEDIAEEADHSKPSPGEAVPENEAIRFFFFSENDDRLRGSNLFWSGMGGSISRNSWEARTSSLLLECRKKHKEAKRKVKAN

Target ORF information:

RefSeq Version	XM_006516956.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleolar protein 8 (Nol8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516956.3

ORF Insert Sequence:

ATGCAAGGCA ACAGAGAAAT GAAGCGCCTC TTTGTGGGTG GACTTGGACA GGGCATTTCT 
GAGACAGACC TACAAAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGACGT GGAGATCATC 
ACTCGGAAAG ATGACCAAGG AAATTCACAG AAAGTCTTTG CGTATGTTAA CATTCAAATA 
ACAGAAGCGG ACCTGAAGAA ATGTATGTCT ATTTTAAACA AAACAAAATG GAAAGGTGGA 
ACACTACAAA TTCAGCTAGC AAAAGAAAGC TTTTTACACA GATTGGCCCA AGAAAGAGAA 
GACGCAAAAG CAAAGAAAGA AAAATCAACA ACAGGCAACC CCACCTTATT GGAAAAGATG 
GGAGCAGTGG ATTTCCATAT GAAGGCTGTG CCTGGAACAG AAGTACCAGG GCATAAAAAT 
TGGGTTGTGA GTAAGTTTGG AAGAGTCTTA CCTGTTCTTC ACCTTAAGAA TCAACAAAAA 
CATAAAATTA TGAAATATGA TCCATCAAAA TACTGCCACA ATATAAAAAA GATTCCAGAG 
AATTTGACAG AAACCACTCC CATAGCTGAA CTCACTTGGG AATTGGAAGG AGGCAATGAC 
CCTATGAGTA AGAAACGTCG AGGAGAGTTC TCTGACTTTC ATATCCCTCC TCAAAAGGTC 
AAGAAAGTGC AGAAGAGCAA TGATCCCATG GAGTCCAAGG TTTCTAATAT TGGTCTAAGG 
ACTAATCAGG TAATGGAGAA GAACAAATCA ACACATCCTG TGACTGCACA CGGGACAGCC 
CCTAGTACTG TTAATCCTTC TAAACAACTC CTTGTGTCCA GTTCTGGTAC TCAGAAACCT 
AAACATGTGG TTTTTCATAA TTCTGACTTT GAAATTATCT GGAATAAAAG TAGCATGTCT 
GATGATGATG TTGACTCGGA AGATGAATTA AAAATGATGA TTGCAAAAGA GGAAAACAGA 
GAGAAACCTG GGCATTCCTC AGTCAATGAG TCTGAACATG ATACTTTTGA AGTTGTTAGG 
GATGATTTCA AATCAAATAT TCACAAGCTT TCTTCTTCAG TAAGCCTAGG AAATAACCAT 
GAATATGATT CAAGTGATAC AGATGAAATT ATTGCAATGA AAACAAAAAA TGCTAAGGTC 
AAAAACAGTG CAGAATCTTC ACAACCAGAA AGGACTGTAA GCAAGAAAAG TTCTTTTCAA 
AAGATAGAAC CTTCTAATGA CTGTATAAAA GTACAAGGAA TTAACAGCAA CAAAGAATCA 
GCCCTGTGTC ATGGAGTAAA GTTTGTAAAT CCCAAATTTC CACCTGACTC CAGTGGCAGT 
GACAGTGAAG AGTCCGAAGA AGATGAAGAA TATAAAGTCT TGATGGAAAA CTGTCCCCGT 
GTGAGTCTCA CTTTGGCTGA CTTGGAGCAG TTGGCTGGCA GTCATCGGAA GTTTCCAGGA 
AAAGACAGTG AGACTAATGG TCCACAAAAT GACAGTCACT GCAAATTTGA CACAACCTCC 
AAGAATCCCA AGACTTCTGG TGACCTGTAC AATGGCAGAC AACAGTGCAT TCTTCCTGAG 
GAGATTGTGG CCTCCCTTTT AGAGGACGAG AATACTTACA GCAAACAAAA ATCGGAAGAA 
GATATCTTAA AGCCAAAATT CCAGGCCTTC AAGGGAATAG GCTGTCTCTA TGCAAAGGAG 
TCAGTAGACA AAACTTTGAA AGAGAATATT GCCTTTAATA CTGGCGGTGG GCATCACAGT 
TCCTTAAAAC ATGAAGATCA CAATAGAAGT TTGATGGAAA ATGGATCTAA GTGTGTTAAT 
GGCTCATCAA GCAAACTGAC TTCATGCCAA CCTGCAAAGA AGGTGAATGA CCCGAACCAC 
ATTCAGCCTC CAAAAAGACA GTGTACTTTT GAGAACCAGA ACCATAAAGT AATGTCCTCT 
ACTAGTTGTG ACAAGGGAAG TACAAATCCT CTCCCAGGTC CGTTGCCATT GAAAGCTAAA 
ACATCCCTGC ATCTTAGTGC CAACAGTCAC AAGGTAGACT CTGATGGAGA CGCTTGCCAT 
TGGCCTGAAA GTAGAAAAGC TTTAGAGAAA GAGAGGACCA ATTTAAGCAA CCTCGAATCT 
CTTGAGAAAT CATCAAAGGT GTCTCCCAGG GAAGACCCTC AGAAAAGCCC AGCTGGTTTC 
TCACTTTCTG ATAGTAATGC ATCCTGTATA AATGCTAAGG ATAAACAAGC TGAAGACAAC 
CAGAAGCGAT TGGCCGCCTT GGCAGCATGG CAGAAAGCCA GGGAAGTACA GAAAAAACTG 
GTGCATAGTG CCCTGGCAAA TTTGGATGGT CATCCAGAGG ACAAAAAAAC ACACATTGTC 
TTTGCTTCTG ATAATGAAAG TGAAACGGAA GAGACATCCA CTCAGGAGCA AAGCTGTCCA 
GAAAAGGAGC TGATGAAAGA ATCTGTGAGT AAATCGCCTG GGAAGCTGTT TGACAGCAGT 
GATGATGAAG ACTCAGATTC CAAGGAGGAC AGCACCAGGT TCAGCATTAA ACCTCAGTTT 
GAGGGCAGAG CTGGCCAGAA GCTTATGGAT CTGCAGTCTC AGTTTGGAAG TGATGAGAGA 
TTCCGCATGG ACTCTAGATT TTTAGAGAGT GACAGTGAGG ACGAAAAGAA AGAGCTAAAT 
GAAGATAAAG TGAATGAAGA CGAGCTTGCT GCAGAAAAAA AGAAAACCCT GAATGTTGTG 
CAAAGTGTTT TGAACATCAA TGTGAACAAC CCGACGAACA AAGGATCAGT GGCTGCTAAG 
AAATTTAAGG ATATCGTACA TTATGACCCA ACAAAGCATG ACCATGCCAT TTATGAAAGA 
AAACAAGAAG ATAAAGAAAA AGAAAGTAAG GCAACACGGA AGAAGAAAAA GGAAGAAGCA 
GAGAAGCTGC CTGAGGTATC CCAAGACATG TATTATAACA TTGCTGCAGA TTTGAAAGAA 
ATATTCCAAA GTATGAGCAA CACAGATGAA AAGGAAGAAG ATGTGCCCAG GACTGAGGCT 
GGTGCTAGAG AGGGGACTGG AAAAATCAGG AACGCCGAGA CACTGGCATG TGAGCCCGAG 
CAAACCACTG GCTTTACATT CTCTTTTTTT GATTCAGCCA CTAAAGATGA AAAGGATGCC 
ACCTATAGAA TCGAACTAGT GAAACATGGG AAGATAGTCT GTCCAAATGA TCCCCGATTC 
CAAGACAGTA GTTCAGAAGA AGAAGATATT GCTGAAGAAG CAGATCATAG CAAGCCAAGC 
CCTGGAGAAG CGGTTCCTGA GAATGAGGCC ATTAGATTTT TCTTTTTCTC TGAGAATGAC 
GACAGACTTC GTGGTTCTAA CTTATTCTGG AGTGGTATGG GAGGTAGTAT TAGCAGAAAT 
TCTTGGGAAG CCCGAACAAG TAGTCTCCTT TTGGAATGTC GGAAGAAACA TAAAGAAGCC 
AAAAGGAAGG TGAAAGCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)
Tarantino C,Paolella G,Cozzuto L,Minopoli G,Pastore L,Parisi S,Russo T

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Nol8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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