PRPF3 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRPF3
Entrez Gene ID	70767
Full Name	pre-mRNA processing factor 3
Synonyms	3632413F13Rik
General protein information	Preferred Names pre-mRNA processing factor 3 Names U4/U6 small nuclear ribonucleoprotein Prp3 PRP3 pre-mRNA processing factor 3 homolog pre-mRNA-splicing factor 3
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006502081.2	XP_006502144.1	U4/U6 small nuclear ribonucleoprotein Prp3 isoform X2
XM_006502081.3	XP_006502144.1	U4/U6 small nuclear ribonucleoprotein Prp3 isoform X1
NM_027541.5	NP_081817.2	U4/U6 small nuclear ribonucleoprotein Prp3
NM_001316751.2	NP_001303680.1	U4/U6 small nuclear ribonucleoprotein Prp3
NM_027541.4	NP_081817.2	U4/U6 small nuclear ribonucleoprotein Prp3
XM_006502080.3	XP_006502143.1	U4/U6 small nuclear ribonucleoprotein Prp3 isoform X1
NM_001316751.1	NP_001303680.1	U4/U6 small nuclear ribonucleoprotein Prp3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRPF3	NP_001233355.1
Caenorhabditis elegans (roundworm)	prp-3	NP_499300.1
Oryza sativa (rice)	Os09g0249600	NP_001062673.1
Homo sapiens (human)	PRPF3	NP_004689.1
	PRPF3	XP_001101105.1
Mus musculus (house mouse)	Prpf3	NP_081817.2
Canis lupus familiaris (dog)	PRPF3	XP_003639679.1
Bos taurus (cattle)	PRPF3	NP_001039516.1
Gallus gallus (chicken)	PRPF3	NP_001026561.1
Danio rerio (zebrafish)	prpf3	NP_991311.1
Neurospora crassa	NCU01637	XP_956005.1
Xenopus tropicalis (tropical clawed frog)	prpf3	NP_001107672.1
Drosophila melanogaster (fruit fly)	Prp3	NP_649156.1
Schizosaccharomyces pombe (fission yeast)	prp3	NP_594560.1
Rattus norvegicus (Norway rat)	Prpf3	NP_001102029.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004682	XP_307259.4
Magnaporthe oryzae (rice blast fungus)	MGG_01157	XP_003717764.1
Arabidopsis thaliana (thale cress)	AT1G28060	NP_174127.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.
Farkas MH, Lew DS, Sousa ME, Bujakowska K, Chatagnon J, Bhattacharya SS, Pierce EA, Nandrot EF
The American journal of pathology184(10)2641-52(2014 Oct)

Alternative splicing and retinal degeneration.
Liu MM, Zack DJ
Clinical genetics84(2)142-9(2013 Aug)

Three gene-targeted mouse models of RNA splicing factor RP show late-onset RPE and retinal degeneration.
Graziotto JJ, Farkas MH, Bujakowska K, Deramaudt BM, Zhang Q, Nandrot EF, Inglehearn CF, Bhattacharya SS, Pierce EA
Investigative ophthalmology & visual science52(1)190-8(2011 Jan)

Genetic variations strongly influence phenotypic outcome in the mouse retina.
Jelcick AS, Yuan Y, Leehy BD, Cox LC, Silveira AC, Qiu F, Schenk S, Sachs AJ, Morrison MA, Nystuen AM, DeAngelis MM, Haider NB
PloS one6(7)e21858(2011)

Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.
Cao H, Wu J, Lam S, Duan R, Newnham C, Molday RS, Graziotto JJ, Pierce EA, Hu J
PloS one6(1)e15860(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mouse retinal pigment epithelium (RPE)is the primary cell affected by mutations in the RNA splicing factors (PRPF3, PRPF8, and PRPF31), and these changes occur at an early age.
Title: Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.

The finding of similar degenerative changes in RPE cells of all three mouse models suggests that the retinal pigment epithelium may be the primary cell type affected in the RNA splicing factor forms of retinitis pigmentosa.
Title: Three gene-targeted mouse models of RNA splicing factor RP show late-onset RPE and retinal degeneration.

analyzed the spatial and temporal expression of the PRPF3 gene in mice and found that it is highly expressed in retinal cells relative to other tissues and its expression is developmentally regulated
Title: Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.

TASP1, EPS15R, and PRPF3 expression were significantly induced in HCCs of transgenic EGF2B mice as was P2 promoter-driven HNF4alpha
Title: EPS15R, TASP1, and PRPF3 are novel disease candidate genes targeted by HNF4alpha splice variants in hepatocellular carcinomas.

The following PRPF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu32287	XM_006502080.3 Latest version!	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu04328	NM_027541.5 Latest version!	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu04328	NM_001316751.2 Latest version!	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu32288	XM_006502081.3 Latest version!	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu32288	XM_006502081.2	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu04328	NM_027541.4	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu04328	NM_001316751.1	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRPF3 ( XM_006502080.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006502143.1 Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu32287
Clone ID Related Accession (Same CDS sequence)	XM_006502080.3
Accession Version	XM_006502080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	U4/U6 small nuclear ribonucleoprotein Prp3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG GCAGCTTCTA GTGATAAAAC TGAAGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502143.1
CDS	1107..2777
Translation	MALSKRELDELKPWIEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAVEEGRSSRHSKSSSDRSRKRELKEVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPSESPGMLTKLQIKQMMEAATRQIEERKKQLSFISPPAPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEVELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLTPPPEPKVRISNLMRVLGTEAVQDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRQLLVIKLKT

Target ORF information:

RefSeq Version	XM_006502080.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502080.3

ORF Insert Sequence:

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG GCAGCTTCTA GTGATAAAAC TGAAGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF3 ( XM_006502081.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006502144.1 Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu32288
Clone ID Related Accession (Same CDS sequence)	XM_006502081.2 , XM_006502081.3
Accession Version	XM_006502081.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	U4/U6 small nuclear ribonucleoprotein Prp3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAACAA AGCTCCAGAT CAAACAGATG ATGGAAGCAG CAACACGACA AATCGAGGAA 
CGGAAAAAAC AGCTGAGCTT CATCAGCCCC CCTGCACCTC AGCCAAAGAC ACCTTCTTCT 
TCCCAACCAG AGCGACTTCC CATCGGCAAC ACTATTCAGC CCTCCCAGGC CGCTACCTTT 
ATGAACGATG CCATTGAGAA GGCGAGGAAA GCTGCCGAGC TGCAAGCCCG AATCCAGGCC 
CAGCTGGCGT TGAAGCCAGG GCTTATTGGC AATGCCAACA TGGTGGGCCT AGCTAATCTC 
CATGCCATGG GCATAGCTCC ACCGAAAGTA GAGTTAAAAG ACCAAACTAA GCCTACCCCA 
TTGATCCTGG ACGAGCAAGG CCGCACTGTG GATGCGACAG GCAAGGAGGT GGAGCTCACA 
CATCGCATGC CCACCCTGAA GGCAAACATT CGGGCTGTGA AGAGGGAACA GTTCAAGCAG 
CAGCTAAAAG AAAAGCCATC AGAAGACATG GAGTCTAATA CCTTTTTTGA CCCCCGAGTC 
TCAATTGCCC CTTCCCAGCG CCAAAGACGC ACTTTTAAAT TCCATGACAA GGGCAAATTT 
GAGAAAATTG CTCAACGATT ACGGACAAAG GCTCAATTGG AGAAGCTGCA AGCAGAGATT 
TCACAAGCTG CTAGAAAAAC AGGCATTCAT ACTTCGACTA GACTTGCCCT CATTGCTCCC 
AAAAAGGAGC TAAAGGAAGG CGATATCCCT GAGATTGAGT GGTGGGACTC GTACATCATA 
CCCAACGGCT TTGACCTTAC AGAGGAAAAT CCCAAGAGAG AAGATTATTT TGGAATCACA 
AATCTTGTTG AACATCCAGC CCAGCTTAAT CCTCCAGTTG ACAATGATAC ACCAGTTACC 
CTTGGGGTAT ATCTTACGAA GAAGGAGCAG AAGAAACTAC GGAGGCAAAC AAGAAGGGAA 
GCACAGAAAG AGCTACAAGA GAAAGTCAGG CTAGGCCTGA CACCTCCTCC AGAGCCCAAA 
GTGAGAATCT CAAATTTAAT GCGAGTATTA GGAACAGAAG CAGTTCAAGA CCCTACAAAG 
GTAGAAGCCC ATGTCAGAGC TCAGATGGCA AAGAGACAGA AAGCGCATGA AGAGGCCAAC 
GCGGCCCGAA AACTTACAGC AGAACAGAGA AAGGTCAAGA AAATTAAAAA GCTTAAAGAA 
GACATTTCAC AGGGGGTGCA CATATCTGTG TATAGAGTTC GAAATTTGAG CAACCCAGCC 
AAGAAGTTCA AGATTGAGGC CAATGCTGGG CAGCTGTACC TGACAGGGGT GGTGGTGCTG 
CACAAAGATG TCAACGTGGT GGTAGTGGAA GGGGGCCCCA AAGCCCAGAA GAAATTTAAG 
CGTCTCATGC TACATCGGAT AAAGTGGGAT GAGCAGACAT CTAACACGAA GGGAGATGAT 
GATGAGGAAT CTGATGAAGA AGCTGTGAAG AAAACTAACA AATGTGTACT AGTATGGGAG 
GGTACAGCCA AAGACCGGAG CTTTGGAGAG ATGAAGTTTA AACAGTGCCC TACAGAGAAC 
ATGGCTCGTG AGCATTTCAA AAAGCATGGG GCTGAACACT ACTGGGACCT TGCACTGAGT 
GAATCTGTGT TGGAGTCCAC CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502144.1
CDS	1608..3254
Translation	MLTKLQIKQMMEAATRQIEERKKQLSFISPPAPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEVELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLTPPPEPKVRISNLMRVLGTEAVQDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRVRNLSNPAKKFKIEANAGQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDEESDEEAVKKTNKCVLVWEGTAKDRSFGEMKFKQCPTENMAREHFKKHGAEHYWDLALSESVLESTD

Target ORF information:

RefSeq Version	XM_006502081.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502081.2

ORF Insert Sequence:

ATGCTAACAA AGCTCCAGAT CAAACAGATG ATGGAAGCAG CAACACGACA AATCGAGGAA 
CGGAAAAAAC AGCTGAGCTT CATCAGCCCC CCTGCACCTC AGCCAAAGAC ACCTTCTTCT 
TCCCAACCAG AGCGACTTCC CATCGGCAAC ACTATTCAGC CCTCCCAGGC CGCTACCTTT 
ATGAACGATG CCATTGAGAA GGCGAGGAAA GCTGCCGAGC TGCAAGCCCG AATCCAGGCC 
CAGCTGGCGT TGAAGCCAGG GCTTATTGGC AATGCCAACA TGGTGGGCCT AGCTAATCTC 
CATGCCATGG GCATAGCTCC ACCGAAAGTA GAGTTAAAAG ACCAAACTAA GCCTACCCCA 
TTGATCCTGG ACGAGCAAGG CCGCACTGTG GATGCGACAG GCAAGGAGGT GGAGCTCACA 
CATCGCATGC CCACCCTGAA GGCAAACATT CGGGCTGTGA AGAGGGAACA GTTCAAGCAG 
CAGCTAAAAG AAAAGCCATC AGAAGACATG GAGTCTAATA CCTTTTTTGA CCCCCGAGTC 
TCAATTGCCC CTTCCCAGCG CCAAAGACGC ACTTTTAAAT TCCATGACAA GGGCAAATTT 
GAGAAAATTG CTCAACGATT ACGGACAAAG GCTCAATTGG AGAAGCTGCA AGCAGAGATT 
TCACAAGCTG CTAGAAAAAC AGGCATTCAT ACTTCGACTA GACTTGCCCT CATTGCTCCC 
AAAAAGGAGC TAAAGGAAGG CGATATCCCT GAGATTGAGT GGTGGGACTC GTACATCATA 
CCCAACGGCT TTGACCTTAC AGAGGAAAAT CCCAAGAGAG AAGATTATTT TGGAATCACA 
AATCTTGTTG AACATCCAGC CCAGCTTAAT CCTCCAGTTG ACAATGATAC ACCAGTTACC 
CTTGGGGTAT ATCTTACGAA GAAGGAGCAG AAGAAACTAC GGAGGCAAAC AAGAAGGGAA 
GCACAGAAAG AGCTACAAGA GAAAGTCAGG CTAGGCCTGA CACCTCCTCC AGAGCCCAAA 
GTGAGAATCT CAAATTTAAT GCGAGTATTA GGAACAGAAG CAGTTCAAGA CCCTACAAAG 
GTAGAAGCCC ATGTCAGAGC TCAGATGGCA AAGAGACAGA AAGCGCATGA AGAGGCCAAC 
GCGGCCCGAA AACTTACAGC AGAACAGAGA AAGGTCAAGA AAATTAAAAA GCTTAAAGAA 
GACATTTCAC AGGGGGTGCA CATATCTGTG TATAGAGTTC GAAATTTGAG CAACCCAGCC 
AAGAAGTTCA AGATTGAGGC CAATGCTGGG CAGCTGTACC TGACAGGGGT GGTGGTGCTG 
CACAAAGATG TCAACGTGGT GGTAGTGGAA GGGGGCCCCA AAGCCCAGAA GAAATTTAAG 
CGTCTCATGC TACATCGGAT AAAGTGGGAT GAGCAGACAT CTAACACGAA GGGAGATGAT 
GATGAGGAAT CTGATGAAGA AGCTGTGAAG AAAACTAACA AATGTGTACT AGTATGGGAG 
GGTACAGCCA AAGACCGGAG CTTTGGAGAG ATGAAGTTTA AACAGTGCCC TACAGAGAAC 
ATGGCTCGTG AGCATTTCAA AAAGCATGGG GCTGAACACT ACTGGGACCT TGCACTGAGT 
GAATCTGTGT TGGAGTCCAC CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF3 ( NM_027541.5 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_081817.2 Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu04328
Clone ID Related Accession (Same CDS sequence)	NM_027541.5 , NM_001316751.2 , NM_027541.4 , NM_001316751.1
Accession Version	NM_027541.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	U4/U6 small nuclear ribonucleoprotein Prp3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093317.22. On Oct 21, 2017 this sequence version replaced NM_027541.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK088194.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG AGTTCGAAAT TTGAGCAACC CAGCCAAGAA GTTCAAGATT 
GAGGCCAATG CTGGGCAGCT GTACCTGACA GGGGTGGTGG TGCTGCACAA AGATGTCAAC 
GTGGTGGTAG TGGAAGGGGG CCCCAAAGCC CAGAAGAAAT TTAAGCGTCT CATGCTACAT 
CGGATAAAGT GGGATGAGCA GACATCTAAC ACGAAGGGAG ATGATGATGA GGAATCTGAT 
GAAGAAGCTG TGAAGAAAAC TAACAAATGT GTACTAGTAT GGGAGGGTAC AGCCAAAGAC 
CGGAGCTTTG GAGAGATGAA GTTTAAACAG TGCCCTACAG AGAACATGGC TCGTGAGCAT 
TTCAAAAAGC ATGGGGCTGA ACACTACTGG GACCTTGCAC TGAGTGAATC TGTGTTGGAG 
TCCACCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081817.2
CDS	484..2535
Translation	MALSKRELDELKPWIEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAVEEGRSSRHSKSSSDRSRKRELKEVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPSESPGMLTKLQIKQMMEAATRQIEERKKQLSFISPPAPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEVELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLTPPPEPKVRISNLMRVLGTEAVQDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRVRNLSNPAKKFKIEANAGQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDEESDEEAVKKTNKCVLVWEGTAKDRSFGEMKFKQCPTENMAREHFKKHGAEHYWDLALSESVLESTD

Target ORF information:

RefSeq Version	NM_027541.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027541.5

ORF Insert Sequence:

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG AGTTCGAAAT TTGAGCAACC CAGCCAAGAA GTTCAAGATT 
GAGGCCAATG CTGGGCAGCT GTACCTGACA GGGGTGGTGG TGCTGCACAA AGATGTCAAC 
GTGGTGGTAG TGGAAGGGGG CCCCAAAGCC CAGAAGAAAT TTAAGCGTCT CATGCTACAT 
CGGATAAAGT GGGATGAGCA GACATCTAAC ACGAAGGGAG ATGATGATGA GGAATCTGAT 
GAAGAAGCTG TGAAGAAAAC TAACAAATGT GTACTAGTAT GGGAGGGTAC AGCCAAAGAC 
CGGAGCTTTG GAGAGATGAA GTTTAAACAG TGCCCTACAG AGAACATGGC TCGTGAGCAT 
TTCAAAAAGC ATGGGGCTGA ACACTACTGG GACCTTGCAC TGAGTGAATC TGTGTTGGAG 
TCCACCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF3 ( NM_001316751.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001303680.1 Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 2, mRNA.

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CloneID	OMu04328
Clone ID Related Accession (Same CDS sequence)	NM_027541.5 , NM_001316751.2 , NM_027541.4 , NM_001316751.1
Accession Version	NM_001316751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	U4/U6 small nuclear ribonucleoprotein Prp3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093317.22, BY308032.1 and AK167435.1. On Oct 21, 2017 this sequence version replaced NM_001316751.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK139852.1, AK167435.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG AGTTCGAAAT TTGAGCAACC CAGCCAAGAA GTTCAAGATT 
GAGGCCAATG CTGGGCAGCT GTACCTGACA GGGGTGGTGG TGCTGCACAA AGATGTCAAC 
GTGGTGGTAG TGGAAGGGGG CCCCAAAGCC CAGAAGAAAT TTAAGCGTCT CATGCTACAT 
CGGATAAAGT GGGATGAGCA GACATCTAAC ACGAAGGGAG ATGATGATGA GGAATCTGAT 
GAAGAAGCTG TGAAGAAAAC TAACAAATGT GTACTAGTAT GGGAGGGTAC AGCCAAAGAC 
CGGAGCTTTG GAGAGATGAA GTTTAAACAG TGCCCTACAG AGAACATGGC TCGTGAGCAT 
TTCAAAAAGC ATGGGGCTGA ACACTACTGG GACCTTGCAC TGAGTGAATC TGTGTTGGAG 
TCCACCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303680.1
CDS	187..2238
Translation	MALSKRELDELKPWIEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAVEEGRSSRHSKSSSDRSRKRELKEVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPSESPGMLTKLQIKQMMEAATRQIEERKKQLSFISPPAPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEVELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLTPPPEPKVRISNLMRVLGTEAVQDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRVRNLSNPAKKFKIEANAGQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDEESDEEAVKKTNKCVLVWEGTAKDRSFGEMKFKQCPTENMAREHFKKHGAEHYWDLALSESVLESTD

Target ORF information:

RefSeq Version	NM_001316751.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316751.2

ORF Insert Sequence:

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG AGTTCGAAAT TTGAGCAACC CAGCCAAGAA GTTCAAGATT 
GAGGCCAATG CTGGGCAGCT GTACCTGACA GGGGTGGTGG TGCTGCACAA AGATGTCAAC 
GTGGTGGTAG TGGAAGGGGG CCCCAAAGCC CAGAAGAAAT TTAAGCGTCT CATGCTACAT 
CGGATAAAGT GGGATGAGCA GACATCTAAC ACGAAGGGAG ATGATGATGA GGAATCTGAT 
GAAGAAGCTG TGAAGAAAAC TAACAAATGT GTACTAGTAT GGGAGGGTAC AGCCAAAGAC 
CGGAGCTTTG GAGAGATGAA GTTTAAACAG TGCCCTACAG AGAACATGGC TCGTGAGCAT 
TTCAAAAAGC ATGGGGCTGA ACACTACTGG GACCTTGCAC TGAGTGAATC TGTGTTGGAG 
TCCACCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF3 ( NM_027541.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_081817.2 Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 1, mRNA.

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CloneID	OMu04328
Clone ID Related Accession (Same CDS sequence)	NM_027541.5 , NM_001316751.2 , NM_027541.4 , NM_001316751.1
Accession Version	NM_027541.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-15
Organism	Mus musculus(House mouse)
Product	U4/U6 small nuclear ribonucleoprotein Prp3
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093317.22. On Apr 30, 2007 this sequence version replaced NM_027541.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK088194.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG AGTTCGAAAT TTGAGCAACC CAGCCAAGAA GTTCAAGATT 
GAGGCCAATG CTGGGCAGCT GTACCTGACA GGGGTGGTGG TGCTGCACAA AGATGTCAAC 
GTGGTGGTAG TGGAAGGGGG CCCCAAAGCC CAGAAGAAAT TTAAGCGTCT CATGCTACAT 
CGGATAAAGT GGGATGAGCA GACATCTAAC ACGAAGGGAG ATGATGATGA GGAATCTGAT 
GAAGAAGCTG TGAAGAAAAC TAACAAATGT GTACTAGTAT GGGAGGGTAC AGCCAAAGAC 
CGGAGCTTTG GAGAGATGAA GTTTAAACAG TGCCCTACAG AGAACATGGC TCGTGAGCAT 
TTCAAAAAGC ATGGGGCTGA ACACTACTGG GACCTTGCAC TGAGTGAATC TGTGTTGGAG 
TCCACCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081817.2
CDS	484..2535
Translation	MALSKRELDELKPWIEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAVEEGRSSRHSKSSSDRSRKRELKEVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPSESPGMLTKLQIKQMMEAATRQIEERKKQLSFISPPAPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEVELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLTPPPEPKVRISNLMRVLGTEAVQDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRVRNLSNPAKKFKIEANAGQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDEESDEEAVKKTNKCVLVWEGTAKDRSFGEMKFKQCPTENMAREHFKKHGAEHYWDLALSESVLESTD

Target ORF information:

RefSeq Version	NM_027541.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027541.4

ORF Insert Sequence:

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG AGTTCGAAAT TTGAGCAACC CAGCCAAGAA GTTCAAGATT 
GAGGCCAATG CTGGGCAGCT GTACCTGACA GGGGTGGTGG TGCTGCACAA AGATGTCAAC 
GTGGTGGTAG TGGAAGGGGG CCCCAAAGCC CAGAAGAAAT TTAAGCGTCT CATGCTACAT 
CGGATAAAGT GGGATGAGCA GACATCTAAC ACGAAGGGAG ATGATGATGA GGAATCTGAT 
GAAGAAGCTG TGAAGAAAAC TAACAAATGT GTACTAGTAT GGGAGGGTAC AGCCAAAGAC 
CGGAGCTTTG GAGAGATGAA GTTTAAACAG TGCCCTACAG AGAACATGGC TCGTGAGCAT 
TTCAAAAAGC ATGGGGCTGA ACACTACTGG GACCTTGCAC TGAGTGAATC TGTGTTGGAG 
TCCACCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF3 ( NM_001316751.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001303680.1 Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 2, mRNA.

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CloneID	OMu04328
Clone ID Related Accession (Same CDS sequence)	NM_027541.5 , NM_001316751.2 , NM_027541.4 , NM_001316751.1
Accession Version	NM_001316751.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-22
Organism	Mus musculus(House mouse)
Product	U4/U6 small nuclear ribonucleoprotein Prp3
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY308032.1 and AK167435.1. On Oct 24, 2015 this sequence version replaced XM_006502079.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK167435.1, AK139852.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG AGTTCGAAAT TTGAGCAACC CAGCCAAGAA GTTCAAGATT 
GAGGCCAATG CTGGGCAGCT GTACCTGACA GGGGTGGTGG TGCTGCACAA AGATGTCAAC 
GTGGTGGTAG TGGAAGGGGG CCCCAAAGCC CAGAAGAAAT TTAAGCGTCT CATGCTACAT 
CGGATAAAGT GGGATGAGCA GACATCTAAC ACGAAGGGAG ATGATGATGA GGAATCTGAT 
GAAGAAGCTG TGAAGAAAAC TAACAAATGT GTACTAGTAT GGGAGGGTAC AGCCAAAGAC 
CGGAGCTTTG GAGAGATGAA GTTTAAACAG TGCCCTACAG AGAACATGGC TCGTGAGCAT 
TTCAAAAAGC ATGGGGCTGA ACACTACTGG GACCTTGCAC TGAGTGAATC TGTGTTGGAG 
TCCACCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303680.1
CDS	184..2235
Translation	MALSKRELDELKPWIEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAVEEGRSSRHSKSSSDRSRKRELKEVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPSESPGMLTKLQIKQMMEAATRQIEERKKQLSFISPPAPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEVELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLTPPPEPKVRISNLMRVLGTEAVQDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRVRNLSNPAKKFKIEANAGQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDEESDEEAVKKTNKCVLVWEGTAKDRSFGEMKFKQCPTENMAREHFKKHGAEHYWDLALSESVLESTD

Target ORF information:

RefSeq Version	NM_001316751.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316751.1

ORF Insert Sequence:

ATGGCGCTGT CTAAGCGGGA GCTGGATGAG CTGAAGCCAT GGATAGAGAA GACGGTGAAG 
AGAGTGCTGG GCTTCTCAGA GCCCACAGTG GTCACTGCAG CCTTGAACTG TGTGGGGAAG 
GGCATGGACA AAAAGAAGGC GGCTGATCAC CTGAAACCTT TTCTTGATGA TTCCACTCTC 
CGATTTGTGG ATAAGCTGTT TGAGGCTGTG GAGGAAGGCC GAAGCTCAAG GCATTCCAAG 
TCTAGCAGTG ACAGAAGCAG AAAGCGAGAG TTAAAGGAGG TGTTTGGTGA TGACTCTGAG 
ATCTCAAAGG AATCATCGGG GGTAAAGAAG CGGCGGATAC CCCGTTTTGA GGAGGTAGAA 
GAGGAGCCAG AGGTGATCCC TGGACCTCCA TCAGAAAGCC CTGGCATGCT AACAAAGCTC 
CAGATCAAAC AGATGATGGA AGCAGCAACA CGACAAATCG AGGAACGGAA AAAACAGCTG 
AGCTTCATCA GCCCCCCTGC ACCTCAGCCA AAGACACCTT CTTCTTCCCA ACCAGAGCGA 
CTTCCCATCG GCAACACTAT TCAGCCCTCC CAGGCCGCTA CCTTTATGAA CGATGCCATT 
GAGAAGGCGA GGAAAGCTGC CGAGCTGCAA GCCCGAATCC AGGCCCAGCT GGCGTTGAAG 
CCAGGGCTTA TTGGCAATGC CAACATGGTG GGCCTAGCTA ATCTCCATGC CATGGGCATA 
GCTCCACCGA AAGTAGAGTT AAAAGACCAA ACTAAGCCTA CCCCATTGAT CCTGGACGAG 
CAAGGCCGCA CTGTGGATGC GACAGGCAAG GAGGTGGAGC TCACACATCG CATGCCCACC 
CTGAAGGCAA ACATTCGGGC TGTGAAGAGG GAACAGTTCA AGCAGCAGCT AAAAGAAAAG 
CCATCAGAAG ACATGGAGTC TAATACCTTT TTTGACCCCC GAGTCTCAAT TGCCCCTTCC 
CAGCGCCAAA GACGCACTTT TAAATTCCAT GACAAGGGCA AATTTGAGAA AATTGCTCAA 
CGATTACGGA CAAAGGCTCA ATTGGAGAAG CTGCAAGCAG AGATTTCACA AGCTGCTAGA 
AAAACAGGCA TTCATACTTC GACTAGACTT GCCCTCATTG CTCCCAAAAA GGAGCTAAAG 
GAAGGCGATA TCCCTGAGAT TGAGTGGTGG GACTCGTACA TCATACCCAA CGGCTTTGAC 
CTTACAGAGG AAAATCCCAA GAGAGAAGAT TATTTTGGAA TCACAAATCT TGTTGAACAT 
CCAGCCCAGC TTAATCCTCC AGTTGACAAT GATACACCAG TTACCCTTGG GGTATATCTT 
ACGAAGAAGG AGCAGAAGAA ACTACGGAGG CAAACAAGAA GGGAAGCACA GAAAGAGCTA 
CAAGAGAAAG TCAGGCTAGG CCTGACACCT CCTCCAGAGC CCAAAGTGAG AATCTCAAAT 
TTAATGCGAG TATTAGGAAC AGAAGCAGTT CAAGACCCTA CAAAGGTAGA AGCCCATGTC 
AGAGCTCAGA TGGCAAAGAG ACAGAAAGCG CATGAAGAGG CCAACGCGGC CCGAAAACTT 
ACAGCAGAAC AGAGAAAGGT CAAGAAAATT AAAAAGCTTA AAGAAGACAT TTCACAGGGG 
GTGCACATAT CTGTGTATAG AGTTCGAAAT TTGAGCAACC CAGCCAAGAA GTTCAAGATT 
GAGGCCAATG CTGGGCAGCT GTACCTGACA GGGGTGGTGG TGCTGCACAA AGATGTCAAC 
GTGGTGGTAG TGGAAGGGGG CCCCAAAGCC CAGAAGAAAT TTAAGCGTCT CATGCTACAT 
CGGATAAAGT GGGATGAGCA GACATCTAAC ACGAAGGGAG ATGATGATGA GGAATCTGAT 
GAAGAAGCTG TGAAGAAAAC TAACAAATGT GTACTAGTAT GGGAGGGTAC AGCCAAAGAC 
CGGAGCTTTG GAGAGATGAA GTTTAAACAG TGCCCTACAG AGAACATGGC TCGTGAGCAT 
TTCAAAAAGC ATGGGGCTGA ACACTACTGG GACCTTGCAC TGAGTGAATC TGTGTTGGAG 
TCCACCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF3 ( XM_006502081.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006502144.1 Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant X1, mRNA.

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CloneID	OMu32288
Clone ID Related Accession (Same CDS sequence)	XM_006502081.2 , XM_006502081.3
Accession Version	XM_006502081.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	U4/U6 small nuclear ribonucleoprotein Prp3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAACAA AGCTCCAGAT CAAACAGATG ATGGAAGCAG CAACACGACA AATCGAGGAA 
CGGAAAAAAC AGCTGAGCTT CATCAGCCCC CCTGCACCTC AGCCAAAGAC ACCTTCTTCT 
TCCCAACCAG AGCGACTTCC CATCGGCAAC ACTATTCAGC CCTCCCAGGC CGCTACCTTT 
ATGAACGATG CCATTGAGAA GGCGAGGAAA GCTGCCGAGC TGCAAGCCCG AATCCAGGCC 
CAGCTGGCGT TGAAGCCAGG GCTTATTGGC AATGCCAACA TGGTGGGCCT AGCTAATCTC 
CATGCCATGG GCATAGCTCC ACCGAAAGTA GAGTTAAAAG ACCAAACTAA GCCTACCCCA 
TTGATCCTGG ACGAGCAAGG CCGCACTGTG GATGCGACAG GCAAGGAGGT GGAGCTCACA 
CATCGCATGC CCACCCTGAA GGCAAACATT CGGGCTGTGA AGAGGGAACA GTTCAAGCAG 
CAGCTAAAAG AAAAGCCATC AGAAGACATG GAGTCTAATA CCTTTTTTGA CCCCCGAGTC 
TCAATTGCCC CTTCCCAGCG CCAAAGACGC ACTTTTAAAT TCCATGACAA GGGCAAATTT 
GAGAAAATTG CTCAACGATT ACGGACAAAG GCTCAATTGG AGAAGCTGCA AGCAGAGATT 
TCACAAGCTG CTAGAAAAAC AGGCATTCAT ACTTCGACTA GACTTGCCCT CATTGCTCCC 
AAAAAGGAGC TAAAGGAAGG CGATATCCCT GAGATTGAGT GGTGGGACTC GTACATCATA 
CCCAACGGCT TTGACCTTAC AGAGGAAAAT CCCAAGAGAG AAGATTATTT TGGAATCACA 
AATCTTGTTG AACATCCAGC CCAGCTTAAT CCTCCAGTTG ACAATGATAC ACCAGTTACC 
CTTGGGGTAT ATCTTACGAA GAAGGAGCAG AAGAAACTAC GGAGGCAAAC AAGAAGGGAA 
GCACAGAAAG AGCTACAAGA GAAAGTCAGG CTAGGCCTGA CACCTCCTCC AGAGCCCAAA 
GTGAGAATCT CAAATTTAAT GCGAGTATTA GGAACAGAAG CAGTTCAAGA CCCTACAAAG 
GTAGAAGCCC ATGTCAGAGC TCAGATGGCA AAGAGACAGA AAGCGCATGA AGAGGCCAAC 
GCGGCCCGAA AACTTACAGC AGAACAGAGA AAGGTCAAGA AAATTAAAAA GCTTAAAGAA 
GACATTTCAC AGGGGGTGCA CATATCTGTG TATAGAGTTC GAAATTTGAG CAACCCAGCC 
AAGAAGTTCA AGATTGAGGC CAATGCTGGG CAGCTGTACC TGACAGGGGT GGTGGTGCTG 
CACAAAGATG TCAACGTGGT GGTAGTGGAA GGGGGCCCCA AAGCCCAGAA GAAATTTAAG 
CGTCTCATGC TACATCGGAT AAAGTGGGAT GAGCAGACAT CTAACACGAA GGGAGATGAT 
GATGAGGAAT CTGATGAAGA AGCTGTGAAG AAAACTAACA AATGTGTACT AGTATGGGAG 
GGTACAGCCA AAGACCGGAG CTTTGGAGAG ATGAAGTTTA AACAGTGCCC TACAGAGAAC 
ATGGCTCGTG AGCATTTCAA AAAGCATGGG GCTGAACACT ACTGGGACCT TGCACTGAGT 
GAATCTGTGT TGGAGTCCAC CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502144.1
CDS	679..2325
Translation	MLTKLQIKQMMEAATRQIEERKKQLSFISPPAPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEVELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLTPPPEPKVRISNLMRVLGTEAVQDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRVRNLSNPAKKFKIEANAGQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDEESDEEAVKKTNKCVLVWEGTAKDRSFGEMKFKQCPTENMAREHFKKHGAEHYWDLALSESVLESTD

Target ORF information:

RefSeq Version	XM_006502081.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-mRNA processing factor 3 (Prpf3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502081.3

ORF Insert Sequence:

ATGCTAACAA AGCTCCAGAT CAAACAGATG ATGGAAGCAG CAACACGACA AATCGAGGAA 
CGGAAAAAAC AGCTGAGCTT CATCAGCCCC CCTGCACCTC AGCCAAAGAC ACCTTCTTCT 
TCCCAACCAG AGCGACTTCC CATCGGCAAC ACTATTCAGC CCTCCCAGGC CGCTACCTTT 
ATGAACGATG CCATTGAGAA GGCGAGGAAA GCTGCCGAGC TGCAAGCCCG AATCCAGGCC 
CAGCTGGCGT TGAAGCCAGG GCTTATTGGC AATGCCAACA TGGTGGGCCT AGCTAATCTC 
CATGCCATGG GCATAGCTCC ACCGAAAGTA GAGTTAAAAG ACCAAACTAA GCCTACCCCA 
TTGATCCTGG ACGAGCAAGG CCGCACTGTG GATGCGACAG GCAAGGAGGT GGAGCTCACA 
CATCGCATGC CCACCCTGAA GGCAAACATT CGGGCTGTGA AGAGGGAACA GTTCAAGCAG 
CAGCTAAAAG AAAAGCCATC AGAAGACATG GAGTCTAATA CCTTTTTTGA CCCCCGAGTC 
TCAATTGCCC CTTCCCAGCG CCAAAGACGC ACTTTTAAAT TCCATGACAA GGGCAAATTT 
GAGAAAATTG CTCAACGATT ACGGACAAAG GCTCAATTGG AGAAGCTGCA AGCAGAGATT 
TCACAAGCTG CTAGAAAAAC AGGCATTCAT ACTTCGACTA GACTTGCCCT CATTGCTCCC 
AAAAAGGAGC TAAAGGAAGG CGATATCCCT GAGATTGAGT GGTGGGACTC GTACATCATA 
CCCAACGGCT TTGACCTTAC AGAGGAAAAT CCCAAGAGAG AAGATTATTT TGGAATCACA 
AATCTTGTTG AACATCCAGC CCAGCTTAAT CCTCCAGTTG ACAATGATAC ACCAGTTACC 
CTTGGGGTAT ATCTTACGAA GAAGGAGCAG AAGAAACTAC GGAGGCAAAC AAGAAGGGAA 
GCACAGAAAG AGCTACAAGA GAAAGTCAGG CTAGGCCTGA CACCTCCTCC AGAGCCCAAA 
GTGAGAATCT CAAATTTAAT GCGAGTATTA GGAACAGAAG CAGTTCAAGA CCCTACAAAG 
GTAGAAGCCC ATGTCAGAGC TCAGATGGCA AAGAGACAGA AAGCGCATGA AGAGGCCAAC 
GCGGCCCGAA AACTTACAGC AGAACAGAGA AAGGTCAAGA AAATTAAAAA GCTTAAAGAA 
GACATTTCAC AGGGGGTGCA CATATCTGTG TATAGAGTTC GAAATTTGAG CAACCCAGCC 
AAGAAGTTCA AGATTGAGGC CAATGCTGGG CAGCTGTACC TGACAGGGGT GGTGGTGCTG 
CACAAAGATG TCAACGTGGT GGTAGTGGAA GGGGGCCCCA AAGCCCAGAA GAAATTTAAG 
CGTCTCATGC TACATCGGAT AAAGTGGGAT GAGCAGACAT CTAACACGAA GGGAGATGAT 
GATGAGGAAT CTGATGAAGA AGCTGTGAAG AAAACTAACA AATGTGTACT AGTATGGGAG 
GGTACAGCCA AAGACCGGAG CTTTGGAGAG ATGAAGTTTA AACAGTGCCC TACAGAGAAC 
ATGGCTCGTG AGCATTTCAA AAAGCATGGG GCTGAACACT ACTGGGACCT TGCACTGAGT 
GAATCTGTGT TGGAGTCCAC CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.
The American journal of pathology184(10)2641-52(2014 Oct)
Farkas MH,Lew DS,Sousa ME,Bujakowska K,Chatagnon J,Bhattacharya SS,Pierce EA,Nandrot EF

Alternative splicing and retinal degeneration.
Clinical genetics84(2)142-9(2013 Aug)
Liu MM,Zack DJ

Three gene-targeted mouse models of RNA splicing factor RP show late-onset RPE and retinal degeneration.
Investigative ophthalmology & visual science52(1)190-8(2011 Jan)
Graziotto JJ,Farkas MH,Bujakowska K,Deramaudt BM,Zhang Q,Nandrot EF,Inglehearn CF,Bhattacharya SS,Pierce EA

Genetic variations strongly influence phenotypic outcome in the mouse retina.
PloS one6(7)e21858(2011)
Jelcick AS,Yuan Y,Leehy BD,Cox LC,Silveira AC,Qiu F,Schenk S,Sachs AJ,Morrison MA,Nystuen AM,DeAngelis MM,Haider NB

Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.
PloS one6(1)e15860(2011 Jan)
Cao H,Wu J,Lam S,Duan R,Newnham C,Molday RS,Graziotto JJ,Pierce EA,Hu J

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PRPF3 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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