Dcp2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dcp2
Entrez Gene ID	70640
Full Name	decapping mRNA 2
Synonyms	2410015D23Rik,5730537H01Rik,AL118268
General protein information	Preferred Names decapping mRNA 2 Names m7GpppN-mRNA hydrolase DCP2 decapping enzyme homolog mRNA-decapping enzyme 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_027490.1	NP_081766.1	m7GpppN-mRNA hydrolase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DCP2	XP_517878.2
Rattus norvegicus (Norway rat)	Dcp2	NP_001163940.1
Homo sapiens (human)	DCP2	NP_689837.2
Canis lupus familiaris (dog)	DCP2	XP_546202.4
Mus musculus (house mouse)	Dcp2	NP_081766.1
Danio rerio (zebrafish)	LOC100534849	XP_003199346.1
Gallus gallus (chicken)	DCP2	XP_004949329.1
Xenopus tropicalis (tropical clawed frog)	dcp2	NP_001016863.1
	DCP2	XP_001082528.1
Danio rerio (zebrafish)	dcp2	NP_956446.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

A long noncoding RNA associated with susceptibility to celiac disease.
Castellanos-Rubio A, Fernandez-Jimenez N, Kratchmarov R, Luo X, Bhagat G, Green PH, Schneider R, Kiledjian M, Bilbao JR, Ghosh S
Science (New York, N.Y.)352(6281)91-5(2016 Apr)

RNA Binding Protein Nanos2 Organizes Post-transcriptional Buffering System to Retain Primitive State of Mouse Spermatogonial Stem Cells.
Zhou Z, Shirakawa T, Ohbo K, Sada A, Wu Q, Hasegawa K, Saba R, Saga Y
Developmental cell34(1)96-107(2015 Jul)

Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion.
Shukla S, Parker R
Proceedings of the National Academy of Sciences of the United States of America111(32)E3277-86(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results showed that DCP1A and DCP2 are critical in the transition from mRNA stability to instability during meiotic maturation and that mRNA degradation must be successful to execute the oocyte-to-zygote transition.
Title: Maternally recruited DCP1A and DCP2 contribute to messenger RNA degradation during oocyte maturation and genome activation in mouse.

Recruitment of DCP1A and DCP2 increases the mRNA degradation capacity of the maturing oocyte so that by the 2-cell stage, most of the maternal mRNA is degraded.
Title: Losing mom's message: requirement for DCP1A and DCP2 in the degradation of maternal transcripts during oocyte maturation.

ERK-phosphorylated Dcp1a enhances its interaction with the decapping enzyme Dcp2 during early differentiation of 3T3-L1 cells.
Title: Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway during early differentiation of 3T3-L1 preadipocytes.

In this study the increase in Irf-7 mRNA within the background of reduced Dcp2 levels was attributed to a stabilization of the Irf-7 mRNA, suggesting that Dcp2 normally modulates Irf-7 mRNA stability.
Title: Dcp2 decapping protein modulates mRNA stability of the critical interferon regulatory factor (IRF) IRF-7.

Like Dcp2, Nudt16 also regulates the stability of a subset of mRNAs including a member of the motin family of proteins involved in angiogenesis.
Title: Multiple mRNA decapping enzymes in mammalian cells.

The following Dcp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dcp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21492	NM_027490.1 Latest version!	Mus musculus decapping mRNA 2 (Dcp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$237.30 $339.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dcp2 ( NM_027490.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081766.1 Mus musculus decapping mRNA 2 (Dcp2), mRNA.

Price & Availability↑

CloneID	OMu21492
Clone ID Related Accession (Same CDS sequence)	NM_027490.1
Accession Version	NM_027490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	m7GpppN-mRNA hydrolase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK153105.1, AK153310.1 and AC147551.5. On or before Jun 8, 2008 this sequence version replaced XM_975674.1, XM_140308.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK152160.1, AK153264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCCA AACGCCTGGA GATTCCCGGC AGCGTCCTGG ATGATCTCTG CAGCCGATTT 
ATTTTGCATA TTCCCAGTGA AGAAAGAGAC AATGCGATCC GAGTGTGCTT CCAGATTGAA 
CTTGCCCATT GGTTTTACTT GGATTTCTAC ATGCAGAACA CACCAGGATT ACCTCAGTGC 
GGGATAAGAG ACTTTGCCAA AGCTGTTTTC AGTCACTGTC CATTTCTGCT GCCACAAGGT 
GAAGATGTGG AAAAGATTTT GGATGAATGG AAGGAATATA AAATGGGAGT CCCGACTTAC 
GGTGCAATTA TTCTCGATGA GACACTGGAG AATGTACTGC TGGTTCAGGG GTACCTGGCA 
AAGTCAGGCT GGGGATTCCC AAAAGGAAAA GTAAATAAAG AAGAGGCGCC TCATGACTGT 
GCGGCGAGAG AGGTCTTCGA GGAAACTGGT TTTGATATAA AAGACTATAT TTGTAAGGAT 
GATTACATTG AACTGCGAAT CAATGACCAG CTTGCTCGCT TGTACATCAT TCCAGGAGTC 
CCAAAAGACA CAAAATTTAA CCCCAAAACC AGAAGAGAAA TTAGGAATAT TGAGTGGTTC 
TCCATTGAGA AATTGCCCTG TCATAGAAAT GACATGACTC CCAAGTCCAA GCTTGGCTTG 
GCACCTAATA AGTTTTTTAT GGCCATTCCC TTTATCAGAC CACTAAGGGA CTGGCTGTCT 
CGAAGATTTG GAGATTCCTC GGACAGTGAC AATGGGTTTT CCTCAGCTGG TAGCACACCA 
GCTAGACCCA CCGTGGAGAA ATTGAGTCGA ACAAAATTCC GCCACAGCCA GCAGCTGTTT 
CCTGAAGGTT CCCCGAGTGA CCAGTGGGTT AAGCACCGGC AGCCACTGCA GCAGAAGTCA 
CACAGTAACC ACGGGGAGGT GTCGGACCTT CTCAAAGCAA AGAATCAAAA TATGAGAGGG 
AATGGCAGAA AACAGTATCA AGACTCACCT AATCAAAAGA AGAGAGCGAA CGGAGTCCAT 
GGTCAGCCGG CAAAGCAGCA GAATCCCTTG GTGAAATGTG AAAAGAAGCT GCATCCACGG 
AAACTTCAGG ACAACTTCGA GACAGATGCC ACATGTGACC TGCCTTGTTC TGGTGAAGAG 
CCGTCGGTAG AACATGCTGA GGGACATTCT GTGGCATGCA ACGGACATTG CAAGTTCCCA 
TTTTCATCCA GAGCCTTTCT GAGTTTCAAG TTTGACCAAA ATGCTATAAT GAAAATCTTG 
GACCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081766.1
CDS	135..1403
Translation	MEPKRLEIPGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKAVFSHCPFLLPQGEDVEKILDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGVPKDTKFNPKTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLSRRFGDSSDSDNGFSSAGSTPARPTVEKLSRTKFRHSQQLFPEGSPSDQWVKHRQPLQQKSHSNHGEVSDLLKAKNQNMRGNGRKQYQDSPNQKKRANGVHGQPAKQQNPLVKCEKKLHPRKLQDNFETDATCDLPCSGEEPSVEHAEGHSVACNGHCKFPFSSRAFLSFKFDQNAIMKILDL

Target ORF information:

RefSeq Version	NM_027490.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus decapping mRNA 2 (Dcp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027490.1

ORF Insert Sequence:

ATGGAGCCCA AACGCCTGGA GATTCCCGGC AGCGTCCTGG ATGATCTCTG CAGCCGATTT 
ATTTTGCATA TTCCCAGTGA AGAAAGAGAC AATGCGATCC GAGTGTGCTT CCAGATTGAA 
CTTGCCCATT GGTTTTACTT GGATTTCTAC ATGCAGAACA CACCAGGATT ACCTCAGTGC 
GGGATAAGAG ACTTTGCCAA AGCTGTTTTC AGTCACTGTC CATTTCTGCT GCCACAAGGT 
GAAGATGTGG AAAAGATTTT GGATGAATGG AAGGAATATA AAATGGGAGT CCCGACTTAC 
GGTGCAATTA TTCTCGATGA GACACTGGAG AATGTACTGC TGGTTCAGGG GTACCTGGCA 
AAGTCAGGCT GGGGATTCCC AAAAGGAAAA GTAAATAAAG AAGAGGCGCC TCATGACTGT 
GCGGCGAGAG AGGTCTTCGA GGAAACTGGT TTTGATATAA AAGACTATAT TTGTAAGGAT 
GATTACATTG AACTGCGAAT CAATGACCAG CTTGCTCGCT TGTACATCAT TCCAGGAGTC 
CCAAAAGACA CAAAATTTAA CCCCAAAACC AGAAGAGAAA TTAGGAATAT TGAGTGGTTC 
TCCATTGAGA AATTGCCCTG TCATAGAAAT GACATGACTC CCAAGTCCAA GCTTGGCTTG 
GCACCTAATA AGTTTTTTAT GGCCATTCCC TTTATCAGAC CACTAAGGGA CTGGCTGTCT 
CGAAGATTTG GAGATTCCTC GGACAGTGAC AATGGGTTTT CCTCAGCTGG TAGCACACCA 
GCTAGACCCA CCGTGGAGAA ATTGAGTCGA ACAAAATTCC GCCACAGCCA GCAGCTGTTT 
CCTGAAGGTT CCCCGAGTGA CCAGTGGGTT AAGCACCGGC AGCCACTGCA GCAGAAGTCA 
CACAGTAACC ACGGGGAGGT GTCGGACCTT CTCAAAGCAA AGAATCAAAA TATGAGAGGG 
AATGGCAGAA AACAGTATCA AGACTCACCT AATCAAAAGA AGAGAGCGAA CGGAGTCCAT 
GGTCAGCCGG CAAAGCAGCA GAATCCCTTG GTGAAATGTG AAAAGAAGCT GCATCCACGG 
AAACTTCAGG ACAACTTCGA GACAGATGCC ACATGTGACC TGCCTTGTTC TGGTGAAGAG 
CCGTCGGTAG AACATGCTGA GGGACATTCT GTGGCATGCA ACGGACATTG CAAGTTCCCA 
TTTTCATCCA GAGCCTTTCT GAGTTTCAAG TTTGACCAAA ATGCTATAAT GAAAATCTTG 
GACCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

A long noncoding RNA associated with susceptibility to celiac disease.
Science (New York, N.Y.)352(6281)91-5(2016 Apr)
Castellanos-Rubio A,Fernandez-Jimenez N,Kratchmarov R,Luo X,Bhagat G,Green PH,Schneider R,Kiledjian M,Bilbao JR,Ghosh S

RNA Binding Protein Nanos2 Organizes Post-transcriptional Buffering System to Retain Primitive State of Mouse Spermatogonial Stem Cells.
Developmental cell34(1)96-107(2015 Jul)
Zhou Z,Shirakawa T,Ohbo K,Sada A,Wu Q,Hasegawa K,Saba R,Saga Y

Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion.
Proceedings of the National Academy of Sciences of the United States of America111(32)E3277-86(2014 Aug)
Shukla S,Parker R