Qpct cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Qpct
Entrez Gene ID	70536
Full Name	glutaminyl-peptide cyclotransferase (glutaminyl cyclase)
Synonyms	5730422A13Rik
General protein information	Preferred Names glutaminyl-peptide cyclotransferase (glutaminyl cyclase) Names glutaminyl-peptide cyclotransferase QC glutaminyl cyclase glutaminyl-tRNA cyclotransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316729.1	NP_001303658.1	glutaminyl-peptide cyclotransferase isoform 2
NM_027455.3	NP_081731.1	glutaminyl-peptide cyclotransferase isoform 1 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	QPCT	XP_004935485.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009673	XP_318733.3
Pan troglodytes (chimpanzee)	QPCT	XP_001167364.1
	QPCT	XP_001101173.2
Danio rerio (zebrafish)	qpctla	NP_001038418.1
Neurospora crassa	NCU11249	XP_001728373.1
Bos taurus (cattle)	QPCT	NP_803472.1
Magnaporthe oryzae (rice blast fungus)	MGG_03692	XP_003716189.1
Xenopus tropicalis (tropical clawed frog)	qpct	NP_001017245.1
Xenopus tropicalis (tropical clawed frog)	LOC101732244	XP_004914940.1
Homo sapiens (human)	QPCT	NP_036545.1
Canis lupus familiaris (dog)	QPCT	XP_005630405.1
Mus musculus (house mouse)	Qpct	NP_081731.1
Rattus norvegicus (Norway rat)	RGD1562284	NP_001128029.1
Danio rerio (zebrafish)	qpctlb	XP_001921878.1
Drosophila melanogaster (fruit fly)	isoQC	NP_788550.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct.
Guo J, He H, Liu Q, Zhang F, Lv J, Zeng T, Gu N, Wu Q
Molecules and cells38(10)859-65(2015 Oct)

Identification of thyrotropin-releasing hormone as hippocampal glutaminyl cyclase substrate in neurons and reactive astrocytes.
Waniek A, Hartlage-R?bsamen M, H?fling C, Kehlen A, Schilling S, Demuth HU, Ro?ner S
Biochimica et biophysica acta1852(1)146-55(2015 Jan)

Mouse strain and brain region-specific expression of the glutaminyl cyclases QC and isoQC.
H?fling C, Indrischek H, H?pcke T, Waniek A, Cynis H, Koch B, Schilling S, Morawski M, Demuth HU, Ro?ner S, Hartlage-R?bsamen M
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience3664-73(2014 Aug)

Selective hippocampal neurodegeneration in transgenic mice expressing small amounts of truncated A? is induced by pyroglutamate-A? formation.
Alexandru A, Jagla W, Graubner S, Becker A, B?uscher C, Kohlmann S, Sedlmeier R, Raber KA, Cynis H, R?nicke R, Reymann KG, Petrasch-Parwez E, Hartlage-R?bsamen M, Waniek A, Rossner S, Schilling S, Osmand AP, Demuth HU, von H?rsten S
The Journal of neuroscience : the official journal of the Society for Neuroscience31(36)12790-801(2011 Sep)

Structures of glycosylated mammalian glutaminyl cyclases reveal conformational variability near the active center.
Ruiz-Carrillo D, Koch B, Parthier C, Wermann M, Dambe T, Buchholz M, Ludwig HH, Heiser U, Rahfeld JU, Stubbs MT, Schilling S, Demuth HU
Biochemistry50(28)6280-8(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors identified a placenta-specific imprinted gene Qpct. Results show that Qpct is widely expressed during early embryonic development and can be detected in the telencephalon, midbrain, and rhombencephalon at E9.5-E11.5.
Title: Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct.

co-regulation of the enzyme glutaminyl cyclase and its substrate TRH was reflected by a co-induction of both proteins in reactive astrocytes in proximity of Abeta deposits
Title: Identification of thyrotropin-releasing hormone as hippocampal glutaminyl cyclase substrate in neurons and reactive astrocytes.

This study provide a thorough comparative characterization of the glutaminyl cyclases QC and isoQC in defined brain regions of different inbred and outbred mouse strains
Title: Mouse strain and brain region-specific expression of the glutaminyl cyclases QC and isoQC.

This study presents a first comparison of two mammalian QCs (mouse and human)containing typical, conserved post-translational modifications.
Title: Structures of glycosylated mammalian glutaminyl cyclases reveal conformational variability near the active center.

Glutaminyl cyclase in a mouse knockout model has a role in hypothyroidism but not hypogonadism
Title: Glutaminyl cyclase knock-out mice exhibit slight hypothyroidism but no hypogonadism: implications for enzyme function and drug development.

The following Qpct gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Qpct cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04238	NM_027455.3 Latest version!	Mus musculus glutaminyl-peptide cyclotransferase (glutaminyl cyclase) (Qpct), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu54001	NM_001316729.1 Latest version!	Mus musculus glutaminyl-peptide cyclotransferase (glutaminyl cyclase) (Qpct), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Qpct ( NM_027455.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081731.1 Mus musculus glutaminyl-peptide cyclotransferase (glutaminyl cyclase) (Qpct), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu04238
Clone ID Related Accession (Same CDS sequence)	NM_027455.3
Accession Version	NM_027455.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	glutaminyl-peptide cyclotransferase isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK045974.1 and AK148394.1. On Oct 24, 2015 this sequence version replaced NM_027455.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK148394.1, AK045974.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGCA GCGAAGACAA GCTCGTCGTG GGCACTCTCC ACCTGCTGCT ACTGCAGGCG 
ACCGTCCTGT CTCTGACAGC TGGGAATCTG AGTCTGGTCT CCGCTGCCTG GACGCAGGAG 
AAGAACCACC ACCAACCAGC ACATTTGAAT TCTTCATCTC TTCAGCAAGT TGCAGAAGGC 
ACTAGCATTT CTGAAATGTG GCAAAACGAC TTGAGACCAT TGCTGATAGA ACGATATCCT 
GGATCGCCCG GAAGCTATTC TGCTCGGCAG CACATCATGC AACGAATTCA GAGACTTCAG 
GCTGAGTGGG TCGTGGAAGT TGACACCTTC CTGAGTAGGA CTCCCTATGG CTATCGGTCC 
TTCTCAAATA TCATCAGCAC TCTTAACCCG GAAGCGAAAC GACACCTGGT CCTCGCCTGC 
CACTACGACT CCAAATATTT TCCTCGATGG GACAGCAGAG TGTTTGTGGG AGCCACGGAT 
TCAGCTGTGC CATGTGCAAT GATGTTGGAA CTTGCCCGTG CCTTAGACAA GAAACTCCAT 
TCCTTGAAGG ATGTCTCTGG TTCCAAGCCA GATCTCTCAC TCCGGCTAAT TTTCTTTGAT 
GGTGAAGAGG CTTTTCATCA CTGGTCCCCT CAAGATTCTC TGTATGGGTC TCGGCACTTA 
GCTCAGAAGA TGGCATCAAG CCCTCACCCT CCTGGATCAA GAGGCACCAA CCAACTGGAT 
GGCATGGATC TGTTGGTCTT ACTAGATTTA ATTGGAGCAG CAAATCCAAC ATTCCCTAAT 
TTTTTCCCCA AGACTACCAG ATGGTTTAAT AGACTTCAAG CAATTGAAAA GGAACTCTAT 
GAATTGGGAT TACTCAAGGA TCATTCTTTG GAGAGGAAGT ATTTTCAGAA TTTTGGCTAT 
GGAAATATTA TCCAGGATGA CCACATTCCA TTTTTAAGAA AAGGTGTCCC AGTTCTTCAC 
CTGATAGCTT CTCCTTTCCC TGAAGTCTGG CACACCATGG ATGACAATGA AGAAAATCTA 
CATGCGTCAA CCATTGACAA TCTCAACAAA ATCATTCAAG TCTTTGTGTT GGAATATCTT 
CACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081731.1
CDS	361..1449
Translation	MAGSEDKLVVGTLHLLLLQATVLSLTAGNLSLVSAAWTQEKNHHQPAHLNSSSLQQVAEGTSISEMWQNDLRPLLIERYPGSPGSYSARQHIMQRIQRLQAEWVVEVDTFLSRTPYGYRSFSNIISTLNPEAKRHLVLACHYDSKYFPRWDSRVFVGATDSAVPCAMMLELARALDKKLHSLKDVSGSKPDLSLRLIFFDGEEAFHHWSPQDSLYGSRHLAQKMASSPHPPGSRGTNQLDGMDLLVLLDLIGAANPTFPNFFPKTTRWFNRLQAIEKELYELGLLKDHSLERKYFQNFGYGNIIQDDHIPFLRKGVPVLHLIASPFPEVWHTMDDNEENLHASTIDNLNKIIQVFVLEYLHL

Target ORF information:

RefSeq Version	NM_027455.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutaminyl-peptide cyclotransferase (glutaminyl cyclase) (Qpct), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027455.3

ORF Insert Sequence:

ATGGCAGGCA GCGAAGACAA GCTCGTCGTG GGCACTCTCC ACCTGCTGCT ACTGCAGGCG 
ACCGTCCTGT CTCTGACAGC TGGGAATCTG AGTCTGGTCT CCGCTGCCTG GACGCAGGAG 
AAGAACCACC ACCAACCAGC ACATTTGAAT TCTTCATCTC TTCAGCAAGT TGCAGAAGGC 
ACTAGCATTT CTGAAATGTG GCAAAACGAC TTGAGACCAT TGCTGATAGA ACGATATCCT 
GGATCGCCCG GAAGCTATTC TGCTCGGCAG CACATCATGC AACGAATTCA GAGACTTCAG 
GCTGAGTGGG TCGTGGAAGT TGACACCTTC CTGAGTAGGA CTCCCTATGG CTATCGGTCC 
TTCTCAAATA TCATCAGCAC TCTTAACCCG GAAGCGAAAC GACACCTGGT CCTCGCCTGC 
CACTACGACT CCAAATATTT TCCTCGATGG GACAGCAGAG TGTTTGTGGG AGCCACGGAT 
TCAGCTGTGC CATGTGCAAT GATGTTGGAA CTTGCCCGTG CCTTAGACAA GAAACTCCAT 
TCCTTGAAGG ATGTCTCTGG TTCCAAGCCA GATCTCTCAC TCCGGCTAAT TTTCTTTGAT 
GGTGAAGAGG CTTTTCATCA CTGGTCCCCT CAAGATTCTC TGTATGGGTC TCGGCACTTA 
GCTCAGAAGA TGGCATCAAG CCCTCACCCT CCTGGATCAA GAGGCACCAA CCAACTGGAT 
GGCATGGATC TGTTGGTCTT ACTAGATTTA ATTGGAGCAG CAAATCCAAC ATTCCCTAAT 
TTTTTCCCCA AGACTACCAG ATGGTTTAAT AGACTTCAAG CAATTGAAAA GGAACTCTAT 
GAATTGGGAT TACTCAAGGA TCATTCTTTG GAGAGGAAGT ATTTTCAGAA TTTTGGCTAT 
GGAAATATTA TCCAGGATGA CCACATTCCA TTTTTAAGAA AAGGTGTCCC AGTTCTTCAC 
CTGATAGCTT CTCCTTTCCC TGAAGTCTGG CACACCATGG ATGACAATGA AGAAAATCTA 
CATGCGTCAA CCATTGACAA TCTCAACAAA ATCATTCAAG TCTTTGTGTT GGAATATCTT 
CACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Qpct ( NM_001316729.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001303658.1 Mus musculus glutaminyl-peptide cyclotransferase (glutaminyl cyclase) (Qpct), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu54001
Clone ID Related Accession (Same CDS sequence)	NM_001316729.1
Accession Version	NM_001316729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	glutaminyl-peptide cyclotransferase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK045974.1, CK624808.1 and AK148394.1. On Oct 24, 2015 this sequence version replaced XM_006524897.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC020023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGCA GCGAAGACAA GCTCGTCGTG GGCACTCTCC ACCTGCTGCT ACTGCAGGCG 
ACCGTCCTGT CTCTGACAGC TGGGAATCTG AGTCTGGTCT CCGCTGCCTG GACGCAGGAG 
AAGCACATCA TGCAACGAAT TCAGAGACTT CAGGCTGAGT GGGTCGTGGA AGTTGACACC 
TTCCTGAGTA GGACTCCCTA TGGCTATCGG TCCTTCTCAA ATATCATCAG CACTCTTAAC 
CCGGAAGCGA AACGACACCT GGTCCTCGCC TGCCACTACG ACTCCAAATA TTTTCCTCGA 
TGGGACAGCA GAGTGTTTGT GGGAGCCACG GATTCAGCTG TGCCATGTGC AATGATGTTG 
GAACTTGCCC GTGCCTTAGA CAAGAAACTC CATTCCTTGA AGGATGTCTC TGGTTCCAAG 
CCAGATCTCT CACTCCGGCT AATTTTCTTT GATGGTGAAG AGGCTTTTCA TCACTGGTCC 
CCTCAAGATT CTCTGTATGG GTCTCGGCAC TTAGCTCAGA AGATGGCATC AAGCCCTCAC 
CCTCCTGGAT CAAGAGGCAC CAACCAACTG GATGGCATGG ATCTGTTGGT CTTACTAGAT 
TTAATTGGAG CAGCAAATCC AACATTCCCT AATTTTTTCC CCAAGACTAC CAGATGGTTT 
AATAGACTTC AAGCAATTGA AAAGGAACTC TATGAATTGG GATTACTCAA GGATCATTCT 
TTGGAGAGGA AGTATTTTCA GAATTTTGGC TATGGAAATA TTATCCAGGA TGACCACATT 
CCATTTTTAA GAAAAGGTGT CCCAGTTCTT CACCTGATAG CTTCTCCTTT CCCTGAAGTC 
TGGCACACCA TGGATGACAA TGAAGAAAAT CTACATGCGT CAACCATTGA CAATCTCAAC 
AAAATCATTC AAGTCTTTGT GTTGGAATAT CTTCACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303658.1
CDS	361..1302
Translation	MAGSEDKLVVGTLHLLLLQATVLSLTAGNLSLVSAAWTQEKHIMQRIQRLQAEWVVEVDTFLSRTPYGYRSFSNIISTLNPEAKRHLVLACHYDSKYFPRWDSRVFVGATDSAVPCAMMLELARALDKKLHSLKDVSGSKPDLSLRLIFFDGEEAFHHWSPQDSLYGSRHLAQKMASSPHPPGSRGTNQLDGMDLLVLLDLIGAANPTFPNFFPKTTRWFNRLQAIEKELYELGLLKDHSLERKYFQNFGYGNIIQDDHIPFLRKGVPVLHLIASPFPEVWHTMDDNEENLHASTIDNLNKIIQVFVLEYLHL

Target ORF information:

RefSeq Version	NM_001316729.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutaminyl-peptide cyclotransferase (glutaminyl cyclase) (Qpct), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316729.1

ORF Insert Sequence:

ATGGCAGGCA GCGAAGACAA GCTCGTCGTG GGCACTCTCC ACCTGCTGCT ACTGCAGGCG 
ACCGTCCTGT CTCTGACAGC TGGGAATCTG AGTCTGGTCT CCGCTGCCTG GACGCAGGAG 
AAGCACATCA TGCAACGAAT TCAGAGACTT CAGGCTGAGT GGGTCGTGGA AGTTGACACC 
TTCCTGAGTA GGACTCCCTA TGGCTATCGG TCCTTCTCAA ATATCATCAG CACTCTTAAC 
CCGGAAGCGA AACGACACCT GGTCCTCGCC TGCCACTACG ACTCCAAATA TTTTCCTCGA 
TGGGACAGCA GAGTGTTTGT GGGAGCCACG GATTCAGCTG TGCCATGTGC AATGATGTTG 
GAACTTGCCC GTGCCTTAGA CAAGAAACTC CATTCCTTGA AGGATGTCTC TGGTTCCAAG 
CCAGATCTCT CACTCCGGCT AATTTTCTTT GATGGTGAAG AGGCTTTTCA TCACTGGTCC 
CCTCAAGATT CTCTGTATGG GTCTCGGCAC TTAGCTCAGA AGATGGCATC AAGCCCTCAC 
CCTCCTGGAT CAAGAGGCAC CAACCAACTG GATGGCATGG ATCTGTTGGT CTTACTAGAT 
TTAATTGGAG CAGCAAATCC AACATTCCCT AATTTTTTCC CCAAGACTAC CAGATGGTTT 
AATAGACTTC AAGCAATTGA AAAGGAACTC TATGAATTGG GATTACTCAA GGATCATTCT 
TTGGAGAGGA AGTATTTTCA GAATTTTGGC TATGGAAATA TTATCCAGGA TGACCACATT 
CCATTTTTAA GAAAAGGTGT CCCAGTTCTT CACCTGATAG CTTCTCCTTT CCCTGAAGTC 
TGGCACACCA TGGATGACAA TGAAGAAAAT CTACATGCGT CAACCATTGA CAATCTCAAC 
AAAATCATTC AAGTCTTTGT GTTGGAATAT CTTCACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct.
Molecules and cells38(10)859-65(2015 Oct)
Guo J,He H,Liu Q,Zhang F,Lv J,Zeng T,Gu N,Wu Q

Identification of thyrotropin-releasing hormone as hippocampal glutaminyl cyclase substrate in neurons and reactive astrocytes.
Biochimica et biophysica acta1852(1)146-55(2015 Jan)
Waniek A,Hartlage-R?bsamen M,H?fling C,Kehlen A,Schilling S,Demuth HU,Ro?ner S

Mouse strain and brain region-specific expression of the glutaminyl cyclases QC and isoQC.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience3664-73(2014 Aug)
H?fling C,Indrischek H,H?pcke T,Waniek A,Cynis H,Koch B,Schilling S,Morawski M,Demuth HU,Ro?ner S,Hartlage-R?bsamen M

Selective hippocampal neurodegeneration in transgenic mice expressing small amounts of truncated A? is induced by pyroglutamate-A? formation.
The Journal of neuroscience : the official journal of the Society for Neuroscience31(36)12790-801(2011 Sep)
Alexandru A,Jagla W,Graubner S,Becker A,B?uscher C,Kohlmann S,Sedlmeier R,Raber KA,Cynis H,R?nicke R,Reymann KG,Petrasch-Parwez E,Hartlage-R?bsamen M,Waniek A,Rossner S,Schilling S,Osmand AP,Demuth HU,von H?rsten S

Structures of glycosylated mammalian glutaminyl cyclases reveal conformational variability near the active center.
Biochemistry50(28)6280-8(2011 Jul)
Ruiz-Carrillo D,Koch B,Parthier C,Wermann M,Dambe T,Buchholz M,Ludwig HH,Heiser U,Rahfeld JU,Stubbs MT,Schilling S,Demuth HU

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Qpct cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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