Wdr77 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wdr77
Entrez Gene ID	70465
Full Name	WD repeat domain 77
Synonyms	2610003I18Rik,2610312E17Rik,C79984,p44/MEP50
General protein information	Preferred Names WD repeat domain 77 Names methylosome protein 50 MEP-50 WD repeat-containing protein 77
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_027432.3	NP_081708.1	methylosome protein 50

Pathways from BioSystems

Homologies

Homo sapiens (human)	WDR77	NP_077007.1
Canis lupus familiaris (dog)	WDR77	XP_537028.1
Pan troglodytes (chimpanzee)	WDR77	NP_001233410.1
Rattus norvegicus (Norway rat)	Wdr77	NP_001008771.2
Gallus gallus (chicken)	WDR77	NP_001026087.1
Mus musculus (house mouse)	Wdr77	NP_081708.1
Xenopus tropicalis (tropical clawed frog)	wdr77	NP_001120009.1
	WDR77	XP_001105103.2
Bos taurus (cattle)	WDR77	NP_001015584.1
Rattus norvegicus (Norway rat)	LOC100911453	XP_003749421.1
Danio rerio (zebrafish)	wdr77	NP_956828.1

Gene Ontology
Bibliography

Related articles in PubMed

PRMT5 is essential for the maintenance of chondrogenic progenitor cells in the limb bud.
Norrie JL, Li Q, Co S, Huang BL, Ding D, Uy JC, Ji Z, Mackem S, Bedford MT, Galli A, Ji H, Vokes SA
Development (Cambridge, England)143(24)4608-4619(2016 12)

E2F and GATA switches turn off WD repeat domain 77 expression in differentiating cells.
Yu M, Wang U, Wang Z
The Biochemical journal473(15)2331-43(2016 08)

cGMP-dependent protein kinase I? interacts with p44/WDR77 to regulate androgen receptor-driven gene expression.
Zhou L, Hosohata K, Gao S, Gu Z, Wang Z
PloS one8(6)e63119(2014)

RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly.
Lo JC, Jamsai D, O'Connor AE, Borg C, Clark BJ, Whisstock JC, Field MC, Adams V, Ishikawa T, Aitken RJ, Whittle B, Goodnow CC, Ormandy CJ, O'Bryan MK
PLoS genetics8(10)e1002969(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WDR77 expression is associated with E2F1, E2F3, GATA2 and GATA6 occupancy on the WDR77 gene, whereas, in contrast, E2F6, GATA1 and GATA3 occupancy is associated with the loss of WDR77 expression during erythroid maturation and lung development
Title: E2F and GATA switches turn off WD repeat domain 77 expression in differentiating cells.

These findings characterize PKG as a novel regulator of AR-mediated transcription by enhancing AR cofactor p44/WDR77's function.
Title: cGMP-dependent protein kinase I? interacts with p44/WDR77 to regulate androgen receptor-driven gene expression.

p44/wdr77 controls cellular proliferation during lung development, and this growth process is reactivated during lung tumorigenesis
Title: The p44/wdr77-dependent cellular proliferation process during lung development is reactivated in lung cancer.

Silencing p21(Cip1) or NF-kappaB p65 expression with short hairpin RNA (shRNA) abolished astrocyte activation and rescued the astrocyte growth inhibition induced by deletion of the p44/WDR77 gene.
Title: Loss of the androgen receptor cofactor p44/WDR77 induces astrogliosis.

These results provide a basis for understanding subcellular transport of p44/WDR77 during prostate development and tumorigenesis.
Title: Nuclear transport signals control cellular localization and function of androgen receptor cofactor p44/WDR77.

The following Wdr77 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wdr77 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18048	NM_027432.3 Latest version!	Mus musculus WD repeat domain 77 (Wdr77), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wdr77 ( NM_027432.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081708.1 Mus musculus WD repeat domain 77 (Wdr77), mRNA.

Price & Availability↑

CloneID	OMu18048
Clone ID Related Accession (Same CDS sequence)	NM_027432.3
Accession Version	NM_027432.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-02
Organism	Mus musculus(house mouse)
Product	methylosome protein 50
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK050391.1. On Jun 9, 2003 this sequence version replaced NM_027432.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK050391.1, SRR1660815.236714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGGAAGG ACACCCCTCC TCCGCTCGTG CCCCCGGCGG CCCGCGAGTG GAACCTGCCC 
CCCAATGCGC CCGCATGCAT GGAACGTCAA TTGGAGGCTG CACGGTACCG GTCTGATGGT 
TCCCTTCTGC TCGGGGTCTC CAGCCTGAGT GGTCGCTGCT GGGTAGGTTC TCTGTGGTTT 
TTCAAGGATC CTAGTGCGGC CCCCAACGAA GGTTTCTGCT CTGCTGGCGT CCAGACCGAG 
GCTGGAGTAG CTGACCTCAC TTGGGTGGGG GACAAAGGTA TCCTAGTGGC TTCTGATTCA 
GGTGCTGTTG AATTGTGGGA GCTAGATGAG AACGAGACAC TTATAGTCAG CAAGTTCTGC 
AAGTATGAGC ATGATGACAT TGTGTCTACT GTCACTGTCC TGAGCTCTGG CACACAAGCT 
GTCAGTGGTA GCAAAGACTG CTGCATCAAA ATTTGGGACC TGGCTCAGCA GGTATCACTG 
AATTCATACC GAGCTCACGC TGGACAGGTT ACCTGTGTTG CTGCCTCTCC CCACAAAGAC 
TCTGTGTTTC TTTCATGTAG TGAGGACAGT AGAATTTTGC TCTGGGATAC CCGCTGTCCC 
AAGCCGGCAT CACAGATGGC CTGCAATGCC TCTGGCTACC TCCCTACCGC TTTGGCTTGG 
CATCCTCAGC AGAGTGAAGT CTTTGTTTTT GGTGACGAGA ATGGATCTGT CTCCCTTGTG 
GACACCAAGA ATGCAAGCTG TACCCTCAGC TCAGCTGTGC ACTCCCAGGG TGTCACTAGA 
CTGGTATTCT CCCCACACAG TGTCCCCCTC CTGACTTCTC TCAGTGAAGA CTGTTCACTT 
GCTGTGCTGG ATTCAAGCCT TTCTGAGGTG TTTAGAAGTC GAGCCCACAG AGACTTTGTG 
AGAGATGCTA CGTGGTCTCC ACTCAATCAC TCCCTTCTTA CCACAGTTGG CTGGGACCAT 
CAGGTCATCC ACCATGTTGT GCCCTTAGAG CCTCTCCCAA ACCCTGGACC TGACAGTGTT 
GTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081708.1
CDS	87..1115
Translation	MRKDTPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGSLLLGVSSLSGRCWVGSLWFFKDPSAAPNEGFCSAGVQTEAGVADLTWVGDKGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVTVLSSGTQAVSGSKDCCIKIWDLAQQVSLNSYRAHAGQVTCVAASPHKDSVFLSCSEDSRILLWDTRCPKPASQMACNASGYLPTALAWHPQQSEVFVFGDENGSVSLVDTKNASCTLSSAVHSQGVTRLVFSPHSVPLLTSLSEDCSLAVLDSSLSEVFRSRAHRDFVRDATWSPLNHSLLTTVGWDHQVIHHVVPLEPLPNPGPDSVVE

Target ORF information:

RefSeq Version	NM_027432.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WD repeat domain 77 (Wdr77), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027432.3

ORF Insert Sequence:

ATGCGGAAGG ACACCCCTCC TCCGCTCGTG CCCCCGGCGG CCCGCGAGTG GAACCTGCCC 
CCCAATGCGC CCGCATGCAT GGAACGTCAA TTGGAGGCTG CACGGTACCG GTCTGATGGT 
TCCCTTCTGC TCGGGGTCTC CAGCCTGAGT GGTCGCTGCT GGGTAGGTTC TCTGTGGTTT 
TTCAAGGATC CTAGTGCGGC CCCCAACGAA GGTTTCTGCT CTGCTGGCGT CCAGACCGAG 
GCTGGAGTAG CTGACCTCAC TTGGGTGGGG GACAAAGGTA TCCTAGTGGC TTCTGATTCA 
GGTGCTGTTG AATTGTGGGA GCTAGATGAG AACGAGACAC TTATAGTCAG CAAGTTCTGC 
AAGTATGAGC ATGATGACAT TGTGTCTACT GTCACTGTCC TGAGCTCTGG CACACAAGCT 
GTCAGTGGTA GCAAAGACTG CTGCATCAAA ATTTGGGACC TGGCTCAGCA GGTATCACTG 
AATTCATACC GAGCTCACGC TGGACAGGTT ACCTGTGTTG CTGCCTCTCC CCACAAAGAC 
TCTGTGTTTC TTTCATGTAG TGAGGACAGT AGAATTTTGC TCTGGGATAC CCGCTGTCCC 
AAGCCGGCAT CACAGATGGC CTGCAATGCC TCTGGCTACC TCCCTACCGC TTTGGCTTGG 
CATCCTCAGC AGAGTGAAGT CTTTGTTTTT GGTGACGAGA ATGGATCTGT CTCCCTTGTG 
GACACCAAGA ATGCAAGCTG TACCCTCAGC TCAGCTGTGC ACTCCCAGGG TGTCACTAGA 
CTGGTATTCT CCCCACACAG TGTCCCCCTC CTGACTTCTC TCAGTGAAGA CTGTTCACTT 
GCTGTGCTGG ATTCAAGCCT TTCTGAGGTG TTTAGAAGTC GAGCCCACAG AGACTTTGTG 
AGAGATGCTA CGTGGTCTCC ACTCAATCAC TCCCTTCTTA CCACAGTTGG CTGGGACCAT 
CAGGTCATCC ACCATGTTGT GCCCTTAGAG CCTCTCCCAA ACCCTGGACC TGACAGTGTT 
GTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PRMT5 is essential for the maintenance of chondrogenic progenitor cells in the limb bud.
Development (Cambridge, England)143(24)4608-4619(2016 12)
Norrie JL,Li Q,Co S,Huang BL,Ding D,Uy JC,Ji Z,Mackem S,Bedford MT,Galli A,Ji H,Vokes SA

E2F and GATA switches turn off WD repeat domain 77 expression in differentiating cells.
The Biochemical journal473(15)2331-43(2016 08)
Yu M,Wang U,Wang Z

cGMP-dependent protein kinase I? interacts with p44/WDR77 to regulate androgen receptor-driven gene expression.
PloS one8(6)e63119(2014)
Zhou L,Hosohata K,Gao S,Gu Z,Wang Z

RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly.
PLoS genetics8(10)e1002969(2012)
Lo JC,Jamsai D,O'Connor AE,Borg C,Clark BJ,Whisstock JC,Field MC,Adams V,Ishikawa T,Aitken RJ,Whittle B,Goodnow CC,Ormandy CJ,O'Bryan MK

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Wdr77 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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