Lipn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lipn
Entrez Gene ID	70166
Full Name	lipase, family member N
Synonyms	2210418G03Rik,Lipl4
General protein information	Preferred Names lipase, family member N Names lipase member N lipase-like abhydrolase domain-containing protein 4 lipase-like, ab-hydrolase domain containing 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527333.2	XP_006527396.1	lipase member N isoform X1
NM_027340.2	NP_081616.1	lipase member N precursor

Pathways from BioSystems

Homologies

	LIPN	XP_001082459.2
Canis lupus familiaris (dog)	LIPN	XP_005636724.1
Bos taurus (cattle)	LIPN	XP_003587914.2
Homo sapiens (human)	LIPN	NP_001095939.1
Pan troglodytes (chimpanzee)	LIPN	XP_521546.3
Mus musculus (house mouse)	Lipn	NP_081616.1
Rattus norvegicus (Norway rat)	Lipn	NP_001178884.1

Gene Ontology
Bibliography

Related articles in PubMed

PNPLA1 defects in patients with autosomal recessive congenital ichthyosis and KO mice sustain PNPLA1 irreplaceable function in epidermal omega-O-acylceramide synthesis and skin permeability barrier.
Pichery M, Huchenq A, Sandhoff R, Severino-Freire M, Zaafouri S, Op?lka L, Levade T, Soldan V, Bertrand-Michel J, Lhuillier E, Serre G, Maruani A, Mazereeuw-Hautier J, Jonca N
Human molecular genetics26(10)1787-1800(2017 05)

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lipn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lipn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04065	NM_027340.2 Latest version!	Mus musculus lipase, family member N (Lipn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu04065	XM_006527333.2 Latest version!	Mus musculus lipase, family member N (Lipn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lipn ( NM_027340.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081616.1 Mus musculus lipase, family member N (Lipn), mRNA.

Price & Availability↑

CloneID	OMu04065
Clone ID Related Accession (Same CDS sequence)	XM_006527333.2 , NM_027340.2
Accession Version	NM_027340.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	lipase member N precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK154333.1. On Apr 6, 2007 this sequence version replaced NM_027340.1. ##Evidence-Data-START## Transcript exon combination :: AK154333.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN00849376 [ECO:0000350] ##Evidence-Data-END##

ATGCCTATGA TGTGGCTATT TTTAACAACG GCTTGTTTGA TACCTGGAAC CTTAAGCGCT 
GGTGGATTCT TGGATTTTGA AAATAAAGTA AATCCTGAAG TGTGGATGAA TGCTAGTGAA 
ATAATCATGT ACAACGGCTA CCCCAGTGAA GAGTATGACG TCACCACCGC AGATGGGTAC 
ATTCTCGCCA TCAACAGAAT TCCCCATGGG AGAGCACAGA CTGGGCAGAC AGGACCCCGG 
CCTGTTGTAT ATATGCAACA TGCCTTGTTT GCAGACAATG CCTACTGGCT TGAGAATTTT 
GCCAATGGAA GCCTAGGGTT CATACTAGCA GATGCAGGTT ATGATGTGTG GATGGGAAAC 
AGTCGAGGAA ACACTTGGTC AAGAAGACAC AAAACTCTCT CAGCAAATGA AGAGAAATTC 
TGGGCTTTTA GCTTCAATGA AATGGCCAAA TATGACCTCC CAGGGATAAT AGATTTCATT 
GTGAATAAAA CAGGTCAGGA GAAGTTGTAC TTCATTGGAC ATTCACTCGG CACTACAATA 
GGATTTGTAG CCTTTTCCAC CATGCCTGAA CTGGCGCAAA GAATCAAAAT GAATTTTGCC 
TTGGGTCCTG TGATCTCATT CAAATATCCT ACAAGCGTTT TTACCAATTT GTTTCTACTT 
CCAAAATCCA TAATCAAGCT TGTGTTTGGC ACCAAAGGTG TCCTTTTAGA AGATAAGAAT 
GCAAGGATGT CATTTATCAC ATTCTGCAAC CAAAAGCTAC TCCAACCATT GTGTAGTGAA 
TTTATGTCCT TGTGGGCTGG ATTCAACAAA AAAAATATGA ATATGAGTCG ACTGGATGTG 
TACATGGCCC ACGCTCCCAC AGGATCATCA ATTCAGAACA TGCTGCACAT AAAGCAGCTT 
TACCGATCTG ATGAATTCAG AGCTTATGAC TGGGGAAGTG AAGCTGAAAA TATGAATCAC 
TACAACCAGA GTTACCCTCC CTTGTATGAC CTGACGGCCA TGAAGGTACC CACTGCCATT 
TGGGCTGGTG GACACGATGT GCTCGTAACT CCTCAAGATG TAGCCAGGAT ACTTCCCCAA 
ATTACAAATC TACGCTACTT CAAGCAATTT CCAGATTGGA ACCATTTTGA TTTTGTCTGG 
GGCCTTGATG CCCCTCAAAG ACTGTACAGT AAAATCATAA GTTTGATGAA AGAATACTTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081616.1
CDS	231..1433
Translation	MPMMWLFLTTACLIPGTLSAGGFLDFENKVNPEVWMNASEIIMYNGYPSEEYDVTTADGYILAINRIPHGRAQTGQTGPRPVVYMQHALFADNAYWLENFANGSLGFILADAGYDVWMGNSRGNTWSRRHKTLSANEEKFWAFSFNEMAKYDLPGIIDFIVNKTGQEKLYFIGHSLGTTIGFVAFSTMPELAQRIKMNFALGPVISFKYPTSVFTNLFLLPKSIIKLVFGTKGVLLEDKNARMSFITFCNQKLLQPLCSEFMSLWAGFNKKNMNMSRLDVYMAHAPTGSSIQNMLHIKQLYRSDEFRAYDWGSEAENMNHYNQSYPPLYDLTAMKVPTAIWAGGHDVLVTPQDVARILPQITNLRYFKQFPDWNHFDFVWGLDAPQRLYSKIISLMKEYF

Target ORF information:

RefSeq Version	NM_027340.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lipase, family member N (Lipn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027340.2

ORF Insert Sequence:

ATGCCTATGA TGTGGCTATT TTTAACAACG GCTTGTTTGA TACCTGGAAC CTTAAGCGCT 
GGTGGATTCT TGGATTTTGA AAATAAAGTA AATCCTGAAG TGTGGATGAA TGCTAGTGAA 
ATAATCATGT ACAACGGCTA CCCCAGTGAA GAGTATGACG TCACCACCGC AGATGGGTAC 
ATTCTCGCCA TCAACAGAAT TCCCCATGGG AGAGCACAGA CTGGGCAGAC AGGACCCCGG 
CCTGTTGTAT ATATGCAACA TGCCTTGTTT GCAGACAATG CCTACTGGCT TGAGAATTTT 
GCCAATGGAA GCCTAGGGTT CATACTAGCA GATGCAGGTT ATGATGTGTG GATGGGAAAC 
AGTCGAGGAA ACACTTGGTC AAGAAGACAC AAAACTCTCT CAGCAAATGA AGAGAAATTC 
TGGGCTTTTA GCTTCAATGA AATGGCCAAA TATGACCTCC CAGGGATAAT AGATTTCATT 
GTGAATAAAA CAGGTCAGGA GAAGTTGTAC TTCATTGGAC ATTCACTCGG CACTACAATA 
GGATTTGTAG CCTTTTCCAC CATGCCTGAA CTGGCGCAAA GAATCAAAAT GAATTTTGCC 
TTGGGTCCTG TGATCTCATT CAAATATCCT ACAAGCGTTT TTACCAATTT GTTTCTACTT 
CCAAAATCCA TAATCAAGCT TGTGTTTGGC ACCAAAGGTG TCCTTTTAGA AGATAAGAAT 
GCAAGGATGT CATTTATCAC ATTCTGCAAC CAAAAGCTAC TCCAACCATT GTGTAGTGAA 
TTTATGTCCT TGTGGGCTGG ATTCAACAAA AAAAATATGA ATATGAGTCG ACTGGATGTG 
TACATGGCCC ACGCTCCCAC AGGATCATCA ATTCAGAACA TGCTGCACAT AAAGCAGCTT 
TACCGATCTG ATGAATTCAG AGCTTATGAC TGGGGAAGTG AAGCTGAAAA TATGAATCAC 
TACAACCAGA GTTACCCTCC CTTGTATGAC CTGACGGCCA TGAAGGTACC CACTGCCATT 
TGGGCTGGTG GACACGATGT GCTCGTAACT CCTCAAGATG TAGCCAGGAT ACTTCCCCAA 
ATTACAAATC TACGCTACTT CAAGCAATTT CCAGATTGGA ACCATTTTGA TTTTGTCTGG 
GGCCTTGATG CCCCTCAAAG ACTGTACAGT AAAATCATAA GTTTGATGAA AGAATACTTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lipn ( XM_006527333.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527396.1 Mus musculus lipase, family member N (Lipn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu04065
Clone ID Related Accession (Same CDS sequence)	XM_006527333.2 , NM_027340.2
Accession Version	XM_006527333.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lipase member N isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006527333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTATGA TGTGGCTATT TTTAACAACG GCTTGTTTGA TACCTGGAAC CTTAAGCGCT 
GGTGGATTCT TGGATTTTGA AAATAAAGTA AATCCTGAAG TGTGGATGAA TGCTAGTGAA 
ATAATCATGT ACAACGGCTA CCCCAGTGAA GAGTATGACG TCACCACCGC AGATGGGTAC 
ATTCTCGCCA TCAACAGAAT TCCCCATGGG AGAGCACAGA CTGGGCAGAC AGGACCCCGG 
CCTGTTGTAT ATATGCAACA TGCCTTGTTT GCAGACAATG CCTACTGGCT TGAGAATTTT 
GCCAATGGAA GCCTAGGGTT CATACTAGCA GATGCAGGTT ATGATGTGTG GATGGGAAAC 
AGTCGAGGAA ACACTTGGTC AAGAAGACAC AAAACTCTCT CAGCAAATGA AGAGAAATTC 
TGGGCTTTTA GCTTCAATGA AATGGCCAAA TATGACCTCC CAGGGATAAT AGATTTCATT 
GTGAATAAAA CAGGTCAGGA GAAGTTGTAC TTCATTGGAC ATTCACTCGG CACTACAATA 
GGATTTGTAG CCTTTTCCAC CATGCCTGAA CTGGCGCAAA GAATCAAAAT GAATTTTGCC 
TTGGGTCCTG TGATCTCATT CAAATATCCT ACAAGCGTTT TTACCAATTT GTTTCTACTT 
CCAAAATCCA TAATCAAGCT TGTGTTTGGC ACCAAAGGTG TCCTTTTAGA AGATAAGAAT 
GCAAGGATGT CATTTATCAC ATTCTGCAAC CAAAAGCTAC TCCAACCATT GTGTAGTGAA 
TTTATGTCCT TGTGGGCTGG ATTCAACAAA AAAAATATGA ATATGAGTCG ACTGGATGTG 
TACATGGCCC ACGCTCCCAC AGGATCATCA ATTCAGAACA TGCTGCACAT AAAGCAGCTT 
TACCGATCTG ATGAATTCAG AGCTTATGAC TGGGGAAGTG AAGCTGAAAA TATGAATCAC 
TACAACCAGA GTTACCCTCC CTTGTATGAC CTGACGGCCA TGAAGGTACC CACTGCCATT 
TGGGCTGGTG GACACGATGT GCTCGTAACT CCTCAAGATG TAGCCAGGAT ACTTCCCCAA 
ATTACAAATC TACGCTACTT CAAGCAATTT CCAGATTGGA ACCATTTTGA TTTTGTCTGG 
GGCCTTGATG CCCCTCAAAG ACTGTACAGT AAAATCATAA GTTTGATGAA AGAATACTTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527396.1
CDS	411..1613
Translation	MPMMWLFLTTACLIPGTLSAGGFLDFENKVNPEVWMNASEIIMYNGYPSEEYDVTTADGYILAINRIPHGRAQTGQTGPRPVVYMQHALFADNAYWLENFANGSLGFILADAGYDVWMGNSRGNTWSRRHKTLSANEEKFWAFSFNEMAKYDLPGIIDFIVNKTGQEKLYFIGHSLGTTIGFVAFSTMPELAQRIKMNFALGPVISFKYPTSVFTNLFLLPKSIIKLVFGTKGVLLEDKNARMSFITFCNQKLLQPLCSEFMSLWAGFNKKNMNMSRLDVYMAHAPTGSSIQNMLHIKQLYRSDEFRAYDWGSEAENMNHYNQSYPPLYDLTAMKVPTAIWAGGHDVLVTPQDVARILPQITNLRYFKQFPDWNHFDFVWGLDAPQRLYSKIISLMKEYF

Target ORF information:

RefSeq Version	XM_006527333.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lipase, family member N (Lipn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527333.2

ORF Insert Sequence:

ATGCCTATGA TGTGGCTATT TTTAACAACG GCTTGTTTGA TACCTGGAAC CTTAAGCGCT 
GGTGGATTCT TGGATTTTGA AAATAAAGTA AATCCTGAAG TGTGGATGAA TGCTAGTGAA 
ATAATCATGT ACAACGGCTA CCCCAGTGAA GAGTATGACG TCACCACCGC AGATGGGTAC 
ATTCTCGCCA TCAACAGAAT TCCCCATGGG AGAGCACAGA CTGGGCAGAC AGGACCCCGG 
CCTGTTGTAT ATATGCAACA TGCCTTGTTT GCAGACAATG CCTACTGGCT TGAGAATTTT 
GCCAATGGAA GCCTAGGGTT CATACTAGCA GATGCAGGTT ATGATGTGTG GATGGGAAAC 
AGTCGAGGAA ACACTTGGTC AAGAAGACAC AAAACTCTCT CAGCAAATGA AGAGAAATTC 
TGGGCTTTTA GCTTCAATGA AATGGCCAAA TATGACCTCC CAGGGATAAT AGATTTCATT 
GTGAATAAAA CAGGTCAGGA GAAGTTGTAC TTCATTGGAC ATTCACTCGG CACTACAATA 
GGATTTGTAG CCTTTTCCAC CATGCCTGAA CTGGCGCAAA GAATCAAAAT GAATTTTGCC 
TTGGGTCCTG TGATCTCATT CAAATATCCT ACAAGCGTTT TTACCAATTT GTTTCTACTT 
CCAAAATCCA TAATCAAGCT TGTGTTTGGC ACCAAAGGTG TCCTTTTAGA AGATAAGAAT 
GCAAGGATGT CATTTATCAC ATTCTGCAAC CAAAAGCTAC TCCAACCATT GTGTAGTGAA 
TTTATGTCCT TGTGGGCTGG ATTCAACAAA AAAAATATGA ATATGAGTCG ACTGGATGTG 
TACATGGCCC ACGCTCCCAC AGGATCATCA ATTCAGAACA TGCTGCACAT AAAGCAGCTT 
TACCGATCTG ATGAATTCAG AGCTTATGAC TGGGGAAGTG AAGCTGAAAA TATGAATCAC 
TACAACCAGA GTTACCCTCC CTTGTATGAC CTGACGGCCA TGAAGGTACC CACTGCCATT 
TGGGCTGGTG GACACGATGT GCTCGTAACT CCTCAAGATG TAGCCAGGAT ACTTCCCCAA 
ATTACAAATC TACGCTACTT CAAGCAATTT CCAGATTGGA ACCATTTTGA TTTTGTCTGG 
GGCCTTGATG CCCCTCAAAG ACTGTACAGT AAAATCATAA GTTTGATGAA AGAATACTTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PNPLA1 defects in patients with autosomal recessive congenital ichthyosis and KO mice sustain PNPLA1 irreplaceable function in epidermal omega-O-acylceramide synthesis and skin permeability barrier.
Human molecular genetics26(10)1787-1800(2017 05)
Pichery M,Huchenq A,Sandhoff R,Severino-Freire M,Zaafouri S,Op?lka L,Levade T,Soldan V,Bertrand-Michel J,Lhuillier E,Serre G,Maruani A,Mazereeuw-Hautier J,Jonca N

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Lipn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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