Slc44a4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc44a4
Entrez Gene ID	70129
Full Name	solute carrier family 44, member 4
Synonyms	2210409B01Rik,NG22,mTPPT1
General protein information	Preferred Names solute carrier family 44, member 4 Names choline transporter-like protein 4 thiamine pyrophosphate transporter 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_023557.3	NP_076046.1	choline transporter-like protein 4
XM_006524885.2	XP_006524948.1	choline transporter-like protein 4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC44A4	NP_079533.2
Bos taurus (cattle)	SLC44A4	NP_001076911.1
Danio rerio (zebrafish)	slc44a4	NP_956707.1
Drosophila melanogaster (fruit fly)	CG11880	NP_651670.1
Anopheles gambiae (African malaria mosquito)	CTLH2_ANOGA	XP_311599.4
Pan troglodytes (chimpanzee)	SLC44A4	XP_003339165.2
Canis lupus familiaris (dog)	SLC44A4	XP_538839.3
	SLC44A4	XP_002803721.1
Mus musculus (house mouse)	Slc44a4	NP_076046.1
Rattus norvegicus (Norway rat)	Slc44a4	NP_997706.1
Xenopus tropicalis (tropical clawed frog)	slc44a4	XP_002941312.2

Gene Ontology
Bibliography

Related articles in PubMed

Mechanism(S) Involved in the Colon-Specific Expression of the Thiamine Pyrophosphate (Tpp) Transporter.
Nabokina SM, Ramos MB, Said HM
PloS one11(2)e0149255(2016)

Molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter.
Nabokina SM, Inoue K, Subramanian VS, Valle JE, Yuasa H, Said HM
The Journal of biological chemistry289(7)4405-16(2014 Feb)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.
Xie T, Rowen L, Aguado B, Ahearn ME, Madan A, Qin S, Campbell RD, Hood L
Genome research13(12)2621-36(2003 Dec)

The mouse as a model for the effects of MHC genes on human disease.
Allcock RJ, Martin AM, Price P
Immunology today21(7)328-32(2000 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

epigenetic mechanisms (histone modifications) play a role in determining the tissue-specific pattern of expression of the TPPT along the GI tract.
Title: Mechanism(S) Involved in the Colon-Specific Expression of the Thiamine Pyrophosphate (Tpp) Transporter.

The following Slc44a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc44a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu53995	XM_006524885.2 Latest version!	Mus musculus solute carrier family 44, member 4 (Slc44a4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu04053	NM_023557.3 Latest version!	Mus musculus solute carrier family 44, member 4 (Slc44a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc44a4 ( XM_006524885.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524948.1 Mus musculus solute carrier family 44, member 4 (Slc44a4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53995
Clone ID Related Accession (Same CDS sequence)	XM_006524885.2
Accession Version	XM_006524885.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	choline transporter-like protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTGTT TCCTTATCAA AGTGCTCCGG TTTCTTCCTG CTGCGGTCCG TAGCAGACCC 
GGGAATTCGG CCAAATATGA CCCCTCCTTT CGAGGCCCCA TCAAAAACAG GGGCTGCACA 
GACATCATCT GCTGTGTCCT CTTCCTGATC TTCATTTTGG GTTACATCAT AGTGGGGCTT 
GTGGCCTGGG TGTATGGAGA CCCTCGGCAA GTGCTCTACC CCAGAAACTC CACGGGGGCC 
TACTGCGGCG TGGGCGACAA CAAGGATAAA CCCTACGTTC TATACTTCGA CATCTTAAGC 
TGTGCGGCAG CTATCAACAT CATCTCCATC GCTGAGAATG GCCTACAGTG CCCCACACCC 
CAGGTGTGCG TCTCTTCCTG CCCACTGGCC CCATGGGCTG TGGAGGTGTT CCAGTTCTCA 
AAGACAGTCG GTGAAGTTTA TGGTGAAAGG AGAAACTTTT GTCTTCCAGC GGTGTCCCCA 
GATATGATAG TGGAGGAGAG CCTGCAGAAG GGACTCTGTC CCCGTTTCCT TCTTCCTTCT 
ACTCCAGCTC TGGGACGTTG TTTCCCACTC CCCAACATCA ACTTCACGTT ACCTGAAGAC 
TTACGGATCA ATAACACCAC CGTCTCTAAT GGCATCAGTG GCCTCCTGGA CAGCATCAAT 
GCCCGGGATG TCTCTGTGAA GATCTTTGAA GATTTTGCCC AGTCCTGGTA CTGGATTCTC 
GTAGCGCTGG GTGTGGCCCT GGCACTAAGT CTGCTGTTCA TCCTCCTCCT GCGCCTGGTG 
GCGGCACCCC TGGTGCTGCT GCTGATTGTC GGGGTGCTCG CTGTGCTCGC CTATGGCATC 
TACCACTGCT GGCAACAGTA CCAAGTGTTT CGGGACAAGG GCGCCTCCAT CACCCAACTG 
GGCTTCACTA CCAACTTCAG TGCCTACCAG AGTGTGAAGG AGACTTGGCT GGCTGCTTTG 
ATTGTCCTGG CTGTCCTGGA GGGCATCTTG TTGCTTATGC TCATTTTCCT GCGCCAGAGG 
ATCCGGATCG CCATCGCTCT GCTGAAGGAA GCCAGCAGGG CTGTGGGGCA GATGATGTCA 
ACTATGTTCT ACCCTCTGGT CACCTTCGTC CTCCTGGTCA TTTGCATTGG TTACTGGGCC 
GTGACAGCTC TATACCTGGC CACATCCGGG CAACCCCAGT ACATCTACTG GGCGTCCAAC 
ACCAGTACAC CGGGCTGTGA GAACGTTCCT GTGAACATGA CTTGTGACCC GATGGCCCCG 
TTGAATTCCT CGTGCCCAAA CCTCAAGTGC GTCTTCAAGG GCTACTCGAC CACGGGCCTA 
GCTCAGCGTT CTCTCTTCAA CCTGCAAATC TACGGAGTCC TGGGGCTCTT CTGGACTGTT 
AACTGGGTAC TGGCCCTGGG CCAGTGTGTC CTTGCCGGGG CCTTCGCCTC TTTCTACTGG 
GCCTTTCACA AGCCTCGAGA CATCCCTACC TTCCCTTTGA GCTCAGCCTT CATCCGCACA 
CTCCGTTACC ACACTGGCTC ACTGGCGTTT GGAGCCCTCA TCCTGTCCCT TGTGCAGATA 
GCCCGGGTTA TCCTGGAGTA TATCGACCAC AAGCTAAGAG GATCCCAGAA CCCTGTGGCC 
CGCTGCATCA TCTGCTGCTT CAAGTGTTGC CTCTGGTGTC TGGAGAAGTT TATTAAGTTC 
CTCAACCGCA ATGCCTACAT CATGATCGCC ATCTATGGAA AGAATTTTTG TGTCTCGGCC 
AAAAATGCCT TCATGCTGCT CATGAGGAAT GTTCTCAGGG TCGTCGTCCT GGATAAAGTC 
ACTGATCTGC TGCTGTTCTT TGGGAAGCTG CTGGTGGTTG GAGGTGTCGG CGTTCTGTCC 
TTCTTCTTTT TCTCCGGTCG TATCAAGGGG CTGGGGAAAG ACTTTGAGAA CCCCAACCTC 
AACTATTATT GGCTGCCCAT CATGACCTCC ATCATGGGAG CCTACGTCAT TGCCAGTGGC 
TTCTTCAGCG TCTTTGGAAT GTGTGTAGAT ACGCTCTTCC TCTGCTTCCT CGAGGACCTG 
GAGAGAAATG ATGGTTCCCA GGAGCGGCCT TACTACATGC CCAAGGCACT CCTGAAGATT 
CTGGGCAAGA AGAATGAGGC ACCTACAGGG GGAAAGACCA GGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524948.1
CDS	16..2166
Translation	MQCFLIKVLRFLPAAVRSRPGNSAKYDPSFRGPIKNRGCTDIICCVLFLIFILGYIIVGLVAWVYGDPRQVLYPRNSTGAYCGVGDNKDKPYVLYFDILSCAAAINIISIAENGLQCPTPQVCVSSCPLAPWAVEVFQFSKTVGEVYGERRNFCLPAVSPDMIVEESLQKGLCPRFLLPSTPALGRCFPLPNINFTLPEDLRINNTTVSNGISGLLDSINARDVSVKIFEDFAQSWYWILVALGVALALSLLFILLLRLVAAPLVLLLIVGVLAVLAYGIYHCWQQYQVFRDKGASITQLGFTTNFSAYQSVKETWLAALIVLAVLEGILLLMLIFLRQRIRIAIALLKEASRAVGQMMSTMFYPLVTFVLLVICIGYWAVTALYLATSGQPQYIYWASNTSTPGCENVPVNMTCDPMAPLNSSCPNLKCVFKGYSTTGLAQRSLFNLQIYGVLGLFWTVNWVLALGQCVLAGAFASFYWAFHKPRDIPTFPLSSAFIRTLRYHTGSLAFGALILSLVQIARVILEYIDHKLRGSQNPVARCIICCFKCCLWCLEKFIKFLNRNAYIMIAIYGKNFCVSAKNAFMLLMRNVLRVVVLDKVTDLLLFFGKLLVVGGVGVLSFFFFSGRIKGLGKDFENPNLNYYWLPIMTSIMGAYVIASGFFSVFGMCVDTLFLCFLEDLERNDGSQERPYYMPKALLKILGKKNEAPTGGKTRKK

Target ORF information:

RefSeq Version	XM_006524885.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 44, member 4 (Slc44a4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524885.2

ORF Insert Sequence:

ATGCAGTGTT TCCTTATCAA AGTGCTCCGG TTTCTTCCTG CTGCGGTCCG TAGCAGACCC 
GGGAATTCGG CCAAATATGA CCCCTCCTTT CGAGGCCCCA TCAAAAACAG GGGCTGCACA 
GACATCATCT GCTGTGTCCT CTTCCTGATC TTCATTTTGG GTTACATCAT AGTGGGGCTT 
GTGGCCTGGG TGTATGGAGA CCCTCGGCAA GTGCTCTACC CCAGAAACTC CACGGGGGCC 
TACTGCGGCG TGGGCGACAA CAAGGATAAA CCCTACGTTC TATACTTCGA CATCTTAAGC 
TGTGCGGCAG CTATCAACAT CATCTCCATC GCTGAGAATG GCCTACAGTG CCCCACACCC 
CAGGTGTGCG TCTCTTCCTG CCCACTGGCC CCATGGGCTG TGGAGGTGTT CCAGTTCTCA 
AAGACAGTCG GTGAAGTTTA TGGTGAAAGG AGAAACTTTT GTCTTCCAGC GGTGTCCCCA 
GATATGATAG TGGAGGAGAG CCTGCAGAAG GGACTCTGTC CCCGTTTCCT TCTTCCTTCT 
ACTCCAGCTC TGGGACGTTG TTTCCCACTC CCCAACATCA ACTTCACGTT ACCTGAAGAC 
TTACGGATCA ATAACACCAC CGTCTCTAAT GGCATCAGTG GCCTCCTGGA CAGCATCAAT 
GCCCGGGATG TCTCTGTGAA GATCTTTGAA GATTTTGCCC AGTCCTGGTA CTGGATTCTC 
GTAGCGCTGG GTGTGGCCCT GGCACTAAGT CTGCTGTTCA TCCTCCTCCT GCGCCTGGTG 
GCGGCACCCC TGGTGCTGCT GCTGATTGTC GGGGTGCTCG CTGTGCTCGC CTATGGCATC 
TACCACTGCT GGCAACAGTA CCAAGTGTTT CGGGACAAGG GCGCCTCCAT CACCCAACTG 
GGCTTCACTA CCAACTTCAG TGCCTACCAG AGTGTGAAGG AGACTTGGCT GGCTGCTTTG 
ATTGTCCTGG CTGTCCTGGA GGGCATCTTG TTGCTTATGC TCATTTTCCT GCGCCAGAGG 
ATCCGGATCG CCATCGCTCT GCTGAAGGAA GCCAGCAGGG CTGTGGGGCA GATGATGTCA 
ACTATGTTCT ACCCTCTGGT CACCTTCGTC CTCCTGGTCA TTTGCATTGG TTACTGGGCC 
GTGACAGCTC TATACCTGGC CACATCCGGG CAACCCCAGT ACATCTACTG GGCGTCCAAC 
ACCAGTACAC CGGGCTGTGA GAACGTTCCT GTGAACATGA CTTGTGACCC GATGGCCCCG 
TTGAATTCCT CGTGCCCAAA CCTCAAGTGC GTCTTCAAGG GCTACTCGAC CACGGGCCTA 
GCTCAGCGTT CTCTCTTCAA CCTGCAAATC TACGGAGTCC TGGGGCTCTT CTGGACTGTT 
AACTGGGTAC TGGCCCTGGG CCAGTGTGTC CTTGCCGGGG CCTTCGCCTC TTTCTACTGG 
GCCTTTCACA AGCCTCGAGA CATCCCTACC TTCCCTTTGA GCTCAGCCTT CATCCGCACA 
CTCCGTTACC ACACTGGCTC ACTGGCGTTT GGAGCCCTCA TCCTGTCCCT TGTGCAGATA 
GCCCGGGTTA TCCTGGAGTA TATCGACCAC AAGCTAAGAG GATCCCAGAA CCCTGTGGCC 
CGCTGCATCA TCTGCTGCTT CAAGTGTTGC CTCTGGTGTC TGGAGAAGTT TATTAAGTTC 
CTCAACCGCA ATGCCTACAT CATGATCGCC ATCTATGGAA AGAATTTTTG TGTCTCGGCC 
AAAAATGCCT TCATGCTGCT CATGAGGAAT GTTCTCAGGG TCGTCGTCCT GGATAAAGTC 
ACTGATCTGC TGCTGTTCTT TGGGAAGCTG CTGGTGGTTG GAGGTGTCGG CGTTCTGTCC 
TTCTTCTTTT TCTCCGGTCG TATCAAGGGG CTGGGGAAAG ACTTTGAGAA CCCCAACCTC 
AACTATTATT GGCTGCCCAT CATGACCTCC ATCATGGGAG CCTACGTCAT TGCCAGTGGC 
TTCTTCAGCG TCTTTGGAAT GTGTGTAGAT ACGCTCTTCC TCTGCTTCCT CGAGGACCTG 
GAGAGAAATG ATGGTTCCCA GGAGCGGCCT TACTACATGC CCAAGGCACT CCTGAAGATT 
CTGGGCAAGA AGAATGAGGC ACCTACAGGG GGAAAGACCA GGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc44a4 ( NM_023557.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076046.1 Mus musculus solute carrier family 44, member 4 (Slc44a4), mRNA.

Price & Availability↑

CloneID	OMu04053
Clone ID Related Accession (Same CDS sequence)	NM_023557.3
Accession Version	NM_023557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	choline transporter-like protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK164684.1, AK145621.1 and AK166620.1. On Feb 2, 2010 this sequence version replaced NM_023557.2. ##Evidence-Data-START## Transcript exon combination :: AK145621.1, BC010808.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGAA AGCAGAATGA GAACGAGGCT CACGGGAATT CGGCCAAATA TGACCCCTCC 
TTTCGAGGCC CCATCAAAAA CAGGGGCTGC ACAGACATCA TCTGCTGTGT CCTCTTCCTG 
ATCTTCATTT TGGGTTACAT CATAGTGGGG CTTGTGGCCT GGGTGTATGG AGACCCTCGG 
CAAGTGCTCT ACCCCAGAAA CTCCACGGGG GCCTACTGCG GCGTGGGCGA CAACAAGGAT 
AAACCCTACG TTCTATACTT CGACATCTTA AGCTGTGCGG CAGCTATCAA CATCATCTCC 
ATCGCTGAGA ATGGCCTACA GTGCCCCACA CCCCAGGTGT GCGTCTCTTC CTGCCCACTG 
GCCCCATGGG CTGTGGAGGT GTTCCAGTTC TCAAAGACAG TCGGTGAAGT TTATGGTGAA 
AGGAGAAACT TTTGTCTTCC AGCGGTGTCC CCAGATATGA TAGTGGAGGA GAGCCTGCAG 
AAGGGACTCT GTCCCCGTTT CCTTCTTCCT TCTACTCCAG CTCTGGGACG TTGTTTCCCA 
CTCCCCAACA TCAACTTCAC GTTACCTGAA GACTTACGGA TCAATAACAC CACCGTCTCT 
AATGGCATCA GTGGCCTCCT GGACAGCATC AATGCCCGGG ATGTCTCTGT GAAGATCTTT 
GAAGATTTTG CCCAGTCCTG GTACTGGATT CTCGTAGCGC TGGGTGTGGC CCTGGCACTA 
AGTCTGCTGT TCATCCTCCT CCTGCGCCTG GTGGCGGCAC CCCTGGTGCT GCTGCTGATT 
GTCGGGGTGC TCGCTGTGCT CGCCTATGGC ATCTACCACT GCTGGCAACA GTACCAAGTG 
TTTCGGGACA AGGGCGCCTC CATCACCCAA CTGGGCTTCA CTACCAACTT CAGTGCCTAC 
CAGAGTGTGA AGGAGACTTG GCTGGCTGCT TTGATTGTCC TGGCTGTCCT GGAGGGCATC 
TTGTTGCTTA TGCTCATTTT CCTGCGCCAG AGGATCCGGA TCGCCATCGC TCTGCTGAAG 
GAAGCCAGCA GGGCTGTGGG GCAGATGATG TCAACTATGT TCTACCCTCT GGTCACCTTC 
GTCCTCCTGG TCATTTGCAT TGGTTACTGG GCCGTGACAG CTCTATACCT GGCCACATCC 
GGGCAACCCC AGTACATCTA CTGGGCGTCC AACACCAGTA CACCGGGCTG TGAGAACGTT 
CCTGTGAACA TGACTTGTGA CCCGATGGCC CCGTTGAATT CCTCGTGCCC AAACCTCAAG 
TGCGTCTTCA AGGGCTACTC GACCACGGGC CTAGCTCAGC GTTCTCTCTT CAACCTGCAA 
ATCTACGGAG TCCTGGGGCT CTTCTGGACT GTTAACTGGG TACTGGCCCT GGGCCAGTGT 
GTCCTTGCCG GGGCCTTCGC CTCTTTCTAC TGGGCCTTTC ACAAGCCTCG AGACATCCCT 
ACCTTCCCTT TGAGCTCAGC CTTCATCCGC ACACTCCGTT ACCACACTGG CTCACTGGCG 
TTTGGAGCCC TCATCCTGTC CCTTGTGCAG ATAGCCCGGG TTATCCTGGA GTATATCGAC 
CACAAGCTAA GAGGATCCCA GAACCCTGTG GCCCGCTGCA TCATCTGCTG CTTCAAGTGT 
TGCCTCTGGT GTCTGGAGAA GTTTATTAAG TTCCTCAACC GCAATGCCTA CATCATGATC 
GCCATCTATG GAAAGAATTT TTGTGTCTCG GCCAAAAATG CCTTCATGCT GCTCATGAGG 
AATGTTCTCA GGGTCGTCGT CCTGGATAAA GTCACTGATC TGCTGCTGTT CTTTGGGAAG 
CTGCTGGTGG TTGGAGGTGT CGGCGTTCTG TCCTTCTTCT TTTTCTCCGG TCGTATCAAG 
GGGCTGGGGA AAGACTTTGA GAACCCCAAC CTCAACTATT ATTGGCTGCC CATCATGACC 
TCCATCATGG GAGCCTACGT CATTGCCAGT GGCTTCTTCA GCGTCTTTGG AATGTGTGTA 
GATACGCTCT TCCTCTGCTT CCTCGAGGAC CTGGAGAGAA ATGATGGTTC CCAGGAGCGG 
CCTTACTACA TGCCCAAGGC ACTCCTGAAG ATTCTGGGCA AGAAGAATGA GGCACCTACA 
GGGGGAAAGA CCAGGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076046.1
CDS	22..2145
Translation	MGRKQNENEAHGNSAKYDPSFRGPIKNRGCTDIICCVLFLIFILGYIIVGLVAWVYGDPRQVLYPRNSTGAYCGVGDNKDKPYVLYFDILSCAAAINIISIAENGLQCPTPQVCVSSCPLAPWAVEVFQFSKTVGEVYGERRNFCLPAVSPDMIVEESLQKGLCPRFLLPSTPALGRCFPLPNINFTLPEDLRINNTTVSNGISGLLDSINARDVSVKIFEDFAQSWYWILVALGVALALSLLFILLLRLVAAPLVLLLIVGVLAVLAYGIYHCWQQYQVFRDKGASITQLGFTTNFSAYQSVKETWLAALIVLAVLEGILLLMLIFLRQRIRIAIALLKEASRAVGQMMSTMFYPLVTFVLLVICIGYWAVTALYLATSGQPQYIYWASNTSTPGCENVPVNMTCDPMAPLNSSCPNLKCVFKGYSTTGLAQRSLFNLQIYGVLGLFWTVNWVLALGQCVLAGAFASFYWAFHKPRDIPTFPLSSAFIRTLRYHTGSLAFGALILSLVQIARVILEYIDHKLRGSQNPVARCIICCFKCCLWCLEKFIKFLNRNAYIMIAIYGKNFCVSAKNAFMLLMRNVLRVVVLDKVTDLLLFFGKLLVVGGVGVLSFFFFSGRIKGLGKDFENPNLNYYWLPIMTSIMGAYVIASGFFSVFGMCVDTLFLCFLEDLERNDGSQERPYYMPKALLKILGKKNEAPTGGKTRKK

Target ORF information:

RefSeq Version	NM_023557.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 44, member 4 (Slc44a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023557.3

ORF Insert Sequence:

ATGGGGAGAA AGCAGAATGA GAACGAGGCT CACGGGAATT CGGCCAAATA TGACCCCTCC 
TTTCGAGGCC CCATCAAAAA CAGGGGCTGC ACAGACATCA TCTGCTGTGT CCTCTTCCTG 
ATCTTCATTT TGGGTTACAT CATAGTGGGG CTTGTGGCCT GGGTGTATGG AGACCCTCGG 
CAAGTGCTCT ACCCCAGAAA CTCCACGGGG GCCTACTGCG GCGTGGGCGA CAACAAGGAT 
AAACCCTACG TTCTATACTT CGACATCTTA AGCTGTGCGG CAGCTATCAA CATCATCTCC 
ATCGCTGAGA ATGGCCTACA GTGCCCCACA CCCCAGGTGT GCGTCTCTTC CTGCCCACTG 
GCCCCATGGG CTGTGGAGGT GTTCCAGTTC TCAAAGACAG TCGGTGAAGT TTATGGTGAA 
AGGAGAAACT TTTGTCTTCC AGCGGTGTCC CCAGATATGA TAGTGGAGGA GAGCCTGCAG 
AAGGGACTCT GTCCCCGTTT CCTTCTTCCT TCTACTCCAG CTCTGGGACG TTGTTTCCCA 
CTCCCCAACA TCAACTTCAC GTTACCTGAA GACTTACGGA TCAATAACAC CACCGTCTCT 
AATGGCATCA GTGGCCTCCT GGACAGCATC AATGCCCGGG ATGTCTCTGT GAAGATCTTT 
GAAGATTTTG CCCAGTCCTG GTACTGGATT CTCGTAGCGC TGGGTGTGGC CCTGGCACTA 
AGTCTGCTGT TCATCCTCCT CCTGCGCCTG GTGGCGGCAC CCCTGGTGCT GCTGCTGATT 
GTCGGGGTGC TCGCTGTGCT CGCCTATGGC ATCTACCACT GCTGGCAACA GTACCAAGTG 
TTTCGGGACA AGGGCGCCTC CATCACCCAA CTGGGCTTCA CTACCAACTT CAGTGCCTAC 
CAGAGTGTGA AGGAGACTTG GCTGGCTGCT TTGATTGTCC TGGCTGTCCT GGAGGGCATC 
TTGTTGCTTA TGCTCATTTT CCTGCGCCAG AGGATCCGGA TCGCCATCGC TCTGCTGAAG 
GAAGCCAGCA GGGCTGTGGG GCAGATGATG TCAACTATGT TCTACCCTCT GGTCACCTTC 
GTCCTCCTGG TCATTTGCAT TGGTTACTGG GCCGTGACAG CTCTATACCT GGCCACATCC 
GGGCAACCCC AGTACATCTA CTGGGCGTCC AACACCAGTA CACCGGGCTG TGAGAACGTT 
CCTGTGAACA TGACTTGTGA CCCGATGGCC CCGTTGAATT CCTCGTGCCC AAACCTCAAG 
TGCGTCTTCA AGGGCTACTC GACCACGGGC CTAGCTCAGC GTTCTCTCTT CAACCTGCAA 
ATCTACGGAG TCCTGGGGCT CTTCTGGACT GTTAACTGGG TACTGGCCCT GGGCCAGTGT 
GTCCTTGCCG GGGCCTTCGC CTCTTTCTAC TGGGCCTTTC ACAAGCCTCG AGACATCCCT 
ACCTTCCCTT TGAGCTCAGC CTTCATCCGC ACACTCCGTT ACCACACTGG CTCACTGGCG 
TTTGGAGCCC TCATCCTGTC CCTTGTGCAG ATAGCCCGGG TTATCCTGGA GTATATCGAC 
CACAAGCTAA GAGGATCCCA GAACCCTGTG GCCCGCTGCA TCATCTGCTG CTTCAAGTGT 
TGCCTCTGGT GTCTGGAGAA GTTTATTAAG TTCCTCAACC GCAATGCCTA CATCATGATC 
GCCATCTATG GAAAGAATTT TTGTGTCTCG GCCAAAAATG CCTTCATGCT GCTCATGAGG 
AATGTTCTCA GGGTCGTCGT CCTGGATAAA GTCACTGATC TGCTGCTGTT CTTTGGGAAG 
CTGCTGGTGG TTGGAGGTGT CGGCGTTCTG TCCTTCTTCT TTTTCTCCGG TCGTATCAAG 
GGGCTGGGGA AAGACTTTGA GAACCCCAAC CTCAACTATT ATTGGCTGCC CATCATGACC 
TCCATCATGG GAGCCTACGT CATTGCCAGT GGCTTCTTCA GCGTCTTTGG AATGTGTGTA 
GATACGCTCT TCCTCTGCTT CCTCGAGGAC CTGGAGAGAA ATGATGGTTC CCAGGAGCGG 
CCTTACTACA TGCCCAAGGC ACTCCTGAAG ATTCTGGGCA AGAAGAATGA GGCACCTACA 
GGGGGAAAGA CCAGGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanism(S) Involved in the Colon-Specific Expression of the Thiamine Pyrophosphate (Tpp) Transporter.
PloS one11(2)e0149255(2016)
Nabokina SM,Ramos MB,Said HM

Molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter.
The Journal of biological chemistry289(7)4405-16(2014 Feb)
Nabokina SM,Inoue K,Subramanian VS,Valle JE,Yuasa H,Said HM

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
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Slc44a4 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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