Spink2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spink2
Entrez Gene ID	69982
Full Name	serine peptidase inhibitor, Kazal type 2
Synonyms	1700007F22Rik,AV038945,HUSI-II
General protein information	Preferred Names serine peptidase inhibitor, Kazal type 2 Names serine protease inhibitor Kazal-type 2 acrosin-trypsin inhibitor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289768.1	NP_001276697.1	serine protease inhibitor Kazal-type 2 isoform 4 precursor
NM_183284.3	NP_899107.1	serine protease inhibitor Kazal-type 2 isoform 2 precursor
NM_001289764.1	NP_001276693.1	serine protease inhibitor Kazal-type 2 isoform 1
XM_011249564.2	XP_011247866.1	serine protease inhibitor Kazal-type 2 isoform X1
NM_001289764.2	NP_001276693.2	serine protease inhibitor Kazal-type 2 isoform 1
NM_001289767.1	NP_001276696.1	serine protease inhibitor Kazal-type 2 isoform 3
XM_011249564.3	XP_011247866.1	serine protease inhibitor Kazal-type 2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Spink2	NP_899107.1
Rattus norvegicus (Norway rat)	LOC100910138	XP_003751398.1
Rattus norvegicus (Norway rat)	Spink2	NP_001008870.1

Gene Ontology
Bibliography

Related articles in PubMed

SPINK2 deficiency causes infertility by inducing sperm defects in heterozygotes and azoospermia in?homozygotes.
Kherraf ZE, Christou-Kent M, Karaouzene T, Amiri-Yekta A, Martinez G, Vargas AS, Lambert E, Borel C, Dorphin B, Aknin-Seifer I, Mitchell MJ, Metzler-Guillemain C, Escoffier J, Nef S, Grepillat M, Thierry-Mieg N, Satre V, Bailly M, Boitrelle F, Pernet-Gallay K, Hennebicq S, Faur? J, Bottari SP, Coutton C, Ray PF, Arnoult C
EMBO molecular medicine9(8)1132-1149(2017 Aug)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Spink2 modulates apoptotic susceptibility and is a candidate gene in the Rgcs1 QTL that affects retinal ganglion cell death after optic nerve damage.
Dietz JA, Maes ME, Huang S, Yandell BS, Schlamp CL, Montgomery AD, Allingham RR, Hauser MA, Nickells RW
PloS one9(4)e93564(2014)

Impaired spermatogenesis and fertility in mice carrying a mutation in the Spink2 gene expressed predominantly in testes.
Lee B, Park I, Jin S, Choi H, Kwon JT, Kim J, Jeong J, Cho BN, Eddy EM, Cho C
The Journal of biological chemistry286(33)29108-17(2011 Aug)

The human (PEDB) and mouse (mPEDB) Prostate Expression Databases.
Nelson PS, Pritchard C, Abbott D, Clegg N
Nucleic acids research30(1)218-20(2002 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of different variants of Spink2 in D407 tissue culture cells increases their susceptibility to the apoptosis-inducing agent staurosporine in a manner consistent with the differential susceptibility between the DBA/2J and BALB/cByJ strains
Title: Spink2 modulates apoptotic susceptibility and is a candidate gene in the Rgcs1 QTL that affects retinal ganglion cell death after optic nerve damage.

SPINK2 is required for maintaining normal spermatogenesis and potentially regulates serine protease-mediated apoptosis in male germ cells.
Title: Impaired spermatogenesis and fertility in mice carrying a mutation in the Spink2 gene expressed predominantly in testes.

The following Spink2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spink2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00014	NM_001289767.1 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu00019	NM_001289768.1 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu00035	NM_001289764.1 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu00025	NM_183284.3 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu84895	NM_001289764.2 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu70484	XM_011249564.3 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu70484	XM_011249564.2	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spink2 ( NM_001289767.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276696.1 Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu00014
Clone ID Related Accession (Same CDS sequence)	NM_001289767.1
Accession Version	NM_001289767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC165975.4. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3, which is shorter at the N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CN843274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGAAGAGGT CACAGTTCCG AACACCAGAC TGTGGTCATT TTGACTTCCC AGCATGCCCT 
AGGAACCTCA ACCCTGTGTG CGGAACGGAT ATGAACACTT ACAGCAATGA ATGTACCCTG 
TGCATGAAAA TCAGGGAGGA CGGTAGCCAT ATTAATATCA TCAAAGACGA GCCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276696.1
CDS	196..375
Translation	MKRSQFRTPDCGHFDFPACPRNLNPVCGTDMNTYSNECTLCMKIREDGSHINIIKDEPC

Target ORF information:

RefSeq Version	NM_001289767.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289767.1

ORF Insert Sequence:

1
61
121

ATGAAGAGGT CACAGTTCCG AACACCAGAC TGTGGTCATT TTGACTTCCC AGCATGCCCT 
AGGAACCTCA ACCCTGTGTG CGGAACGGAT ATGAACACTT ACAGCAATGA ATGTACCCTG 
TGCATGAAAA TCAGGGAGGA CGGTAGCCAT ATTAATATCA TCAAAGACGA GCCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink2 ( NM_001289768.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276697.1 Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu00019
Clone ID Related Accession (Same CDS sequence)	NM_001289768.1
Accession Version	NM_001289768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 2 isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV068802.1, AK005709.1 and AV380723.1. Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region and uses an alternate splice site in the central coding region, resulting in a frameshift and an early stop codon, compared to variant 1. It encodes isoform 4, which is shorter and has distinct N- and C-termini, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC048619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGCTGAGAC TGGTGCTGTT GCTCCTGGTC ACAGACTTTG CAGCCTCTCA TGAGACTCTC 
GACTCTTCCG ATTCTCAAAT CATGAAGAGG TCACAGTTCC GAACAACTGT GGTCATTTTG 
ACTTCCCAGC ATGCCCTAGG AACCTCAACC CTGTGTGCGG AACGGATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276697.1
CDS	98..268
Translation	MLRLVLLLLVTDFAASHETLDSSDSQIMKRSQFRTTVVILTSQHALGTSTLCAERI

Target ORF information:

RefSeq Version	NM_001289768.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289768.1

ORF Insert Sequence:

1
61
121

ATGCTGAGAC TGGTGCTGTT GCTCCTGGTC ACAGACTTTG CAGCCTCTCA TGAGACTCTC 
GACTCTTCCG ATTCTCAAAT CATGAAGAGG TCACAGTTCC GAACAACTGT GGTCATTTTG 
ACTTCCCAGC ATGCCCTAGG AACCTCAACC CTGTGTGCGG AACGGATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink2 ( NM_001289764.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276693.1 Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00035
Clone ID Related Accession (Same CDS sequence)	NM_001289764.1
Accession Version	NM_001289764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC165975.4. On Jan 29, 2014 this sequence version replaced XM_006535215.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BU937354.1, CA465079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTCGT GCCGTGCCGT TACTGGCGGT TCTGCCCCGT GGAGAGTGGT GGCCATGGAA 
GCCTCTCATG AGACTCTCGA CTCTTCCGAT TCTCAAATCA TGAAGAGGTC ACAGTTCCGA 
ACACCAGACT GTGGTCATTT TGACTTCCCA GCATGCCCTA GGAACCTCAA CCCTGTGTGC 
GGAACGGATA TGAACACTTA CAGCAATGAA TGTACCCTGT GCATGAAAAT CAGGGAGGAC 
GGTAGCCATA TTAATATCAT CAAAGACGAG CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276693.1
CDS	1..279
Translation	MGSCRAVTGGSAPWRVVAMEASHETLDSSDSQIMKRSQFRTPDCGHFDFPACPRNLNPVCGTDMNTYSNECTLCMKIREDGSHINIIKDEPC

Target ORF information:

RefSeq Version	NM_001289764.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289764.1

ORF Insert Sequence:

ATGGGTTCGT GCCGTGCCGT TACTGGCGGT TCTGCCCCGT GGAGAGTGGT GGCCATGGAA 
GCCTCTCATG AGACTCTCGA CTCTTCCGAT TCTCAAATCA TGAAGAGGTC ACAGTTCCGA 
ACACCAGACT GTGGTCATTT TGACTTCCCA GCATGCCCTA GGAACCTCAA CCCTGTGTGC 
GGAACGGATA TGAACACTTA CAGCAATGAA TGTACCCTGT GCATGAAAAT CAGGGAGGAC 
GGTAGCCATA TTAATATCAT CAAAGACGAG CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink2 ( XM_011249564.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247866.1 Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70484
Clone ID Related Accession (Same CDS sequence)	XM_011249564.2 , XM_011249564.3
Accession Version	XM_011249564.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTGG TCCTGATGAG GGCTGAGTAC TCAGCCGCTG CTTTCTCAGC CTCTCATGAG 
ACTCTCGACT CTTCCGATTC TCAAATCATG AAGAGGTCAC AGTTCCGAAC ACCAGACTGT 
GGTCATTTTG ACTTCCCAGC ATGCCCTAGG AACCTCAACC CTGTGTGCGG AACGGATATG 
AACACTTACA GCAATGAATG TACCCTGTGC ATGAAAATCA GGGAGGACGG TAGCCATATT 
AATATCATCA AAGACGAGCC ATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247866.1
CDS	36..302
Translation	MSLVLMRAEYSAAAFSASHETLDSSDSQIMKRSQFRTPDCGHFDFPACPRNLNPVCGTDMNTYSNECTLCMKIREDGSHINIIKDEPC

Target ORF information:

RefSeq Version	XM_011249564.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249564.2

ORF Insert Sequence:

ATGTCGTTGG TCCTGATGAG GGCTGAGTAC TCAGCCGCTG CTTTCTCAGC CTCTCATGAG 
ACTCTCGACT CTTCCGATTC TCAAATCATG AAGAGGTCAC AGTTCCGAAC ACCAGACTGT 
GGTCATTTTG ACTTCCCAGC ATGCCCTAGG AACCTCAACC CTGTGTGCGG AACGGATATG 
AACACTTACA GCAATGAATG TACCCTGTGC ATGAAAATCA GGGAGGACGG TAGCCATATT 
AATATCATCA AAGACGAGCC ATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink2 ( NM_183284.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_899107.1 Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00025
Clone ID Related Accession (Same CDS sequence)	NM_183284.3
Accession Version	NM_183284.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-19
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 2 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV068802.1, AK005709.1 and AV380723.1. On Jan 29, 2014 this sequence version replaced NM_183284.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which is longer and has a distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: DV068802.1, CN838365.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGAC TGGTGCTGTT GCTCCTGGTC ACAGACTTTG CAGCCTCTCA TGAGACTCTC 
GACTCTTCCG ATTCTCAAAT CATGAAGAGG TCACAGTTCC GAACACCAGA CTGTGGTCAT 
TTTGACTTCC CAGCATGCCC TAGGAACCTC AACCCTGTGT GCGGAACGGA TATGAACACT 
TACAGCAATG AATGTACCCT GTGCATGAAA ATCAGGGAGG ACGGTAGCCA TATTAATATC 
ATCAAAGACG AGCCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899107.1
CDS	98..358
Translation	MLRLVLLLLVTDFAASHETLDSSDSQIMKRSQFRTPDCGHFDFPACPRNLNPVCGTDMNTYSNECTLCMKIREDGSHINIIKDEPC

Target ORF information:

RefSeq Version	NM_183284.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183284.3

ORF Insert Sequence:

ATGCTGAGAC TGGTGCTGTT GCTCCTGGTC ACAGACTTTG CAGCCTCTCA TGAGACTCTC 
GACTCTTCCG ATTCTCAAAT CATGAAGAGG TCACAGTTCC GAACACCAGA CTGTGGTCAT 
TTTGACTTCC CAGCATGCCC TAGGAACCTC AACCCTGTGT GCGGAACGGA TATGAACACT 
TACAGCAATG AATGTACCCT GTGCATGAAA ATCAGGGAGG ACGGTAGCCA TATTAATATC 
ATCAAAGACG AGCCATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink2 ( NM_001289764.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276693.2 Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu84895
Clone ID Related Accession (Same CDS sequence)	NM_001289764.2
Accession Version	NM_001289764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-19
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC165975.4. On Jun 20, 2019 this sequence version replaced NM_001289764.1. Transcript Variant: This variant (1) encodes the isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BU937354.1, CA465079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCCT CTCATGAGAC TCTCGACTCT TCCGATTCTC AAATCATGAA GAGGTCACAG 
TTCCGAACAC CAGACTGTGG TCATTTTGAC TTCCCAGCAT GCCCTAGGAA CCTCAACCCT 
GTGTGCGGAA CGGATATGAA CACTTACAGC AATGAATGTA CCCTGTGCAT GAAAATCAGG 
GAGGACGGTA GCCATATTAA TATCATCAAA GACGAGCCAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276693.2
CDS	34..258
Translation	MEASHETLDSSDSQIMKRSQFRTPDCGHFDFPACPRNLNPVCGTDMNTYSNECTLCMKIREDGSHINIIKDEPC

Target ORF information:

RefSeq Version	NM_001289764.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289764.2

ORF Insert Sequence:

ATGGAAGCCT CTCATGAGAC TCTCGACTCT TCCGATTCTC AAATCATGAA GAGGTCACAG 
TTCCGAACAC CAGACTGTGG TCATTTTGAC TTCCCAGCAT GCCCTAGGAA CCTCAACCCT 
GTGTGCGGAA CGGATATGAA CACTTACAGC AATGAATGTA CCCTGTGCAT GAAAATCAGG 
GAGGACGGTA GCCATATTAA TATCATCAAA GACGAGCCAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spink2 ( XM_011249564.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247866.1 Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70484
Clone ID Related Accession (Same CDS sequence)	XM_011249564.2 , XM_011249564.3
Accession Version	XM_011249564.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTGG TCCTGATGAG GGCTGAGTAC TCAGCCGCTG CTTTCTCAGC CTCTCATGAG 
ACTCTCGACT CTTCCGATTC TCAAATCATG AAGAGGTCAC AGTTCCGAAC ACCAGACTGT 
GGTCATTTTG ACTTCCCAGC ATGCCCTAGG AACCTCAACC CTGTGTGCGG AACGGATATG 
AACACTTACA GCAATGAATG TACCCTGTGC ATGAAAATCA GGGAGGACGG TAGCCATATT 
AATATCATCA AAGACGAGCC ATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247866.1
CDS	37..303
Translation	MSLVLMRAEYSAAAFSASHETLDSSDSQIMKRSQFRTPDCGHFDFPACPRNLNPVCGTDMNTYSNECTLCMKIREDGSHINIIKDEPC

Target ORF information:

RefSeq Version	XM_011249564.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249564.3

ORF Insert Sequence:

ATGTCGTTGG TCCTGATGAG GGCTGAGTAC TCAGCCGCTG CTTTCTCAGC CTCTCATGAG 
ACTCTCGACT CTTCCGATTC TCAAATCATG AAGAGGTCAC AGTTCCGAAC ACCAGACTGT 
GGTCATTTTG ACTTCCCAGC ATGCCCTAGG AACCTCAACC CTGTGTGCGG AACGGATATG 
AACACTTACA GCAATGAATG TACCCTGTGC ATGAAAATCA GGGAGGACGG TAGCCATATT 
AATATCATCA AAGACGAGCC ATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPINK2 deficiency causes infertility by inducing sperm defects in heterozygotes and azoospermia in?homozygotes.
EMBO molecular medicine9(8)1132-1149(2017 Aug)
Kherraf ZE,Christou-Kent M,Karaouzene T,Amiri-Yekta A,Martinez G,Vargas AS,Lambert E,Borel C,Dorphin B,Aknin-Seifer I,Mitchell MJ,Metzler-Guillemain C,Escoffier J,Nef S,Grepillat M,Thierry-Mieg N,Satre V,Bailly M,Boitrelle F,Pernet-Gallay K,Hennebicq S,Faur? J,Bottari SP,Coutton C,Ray PF,Arnoult C

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Spink2 modulates apoptotic susceptibility and is a candidate gene in the Rgcs1 QTL that affects retinal ganglion cell death after optic nerve damage.
PloS one9(4)e93564(2014)
Dietz JA,Maes ME,Huang S,Yandell BS,Schlamp CL,Montgomery AD,Allingham RR,Hauser MA,Nickells RW

Impaired spermatogenesis and fertility in mice carrying a mutation in the Spink2 gene expressed predominantly in testes.
The Journal of biological chemistry286(33)29108-17(2011 Aug)
Lee B,Park I,Jin S,Choi H,Kwon JT,Kim J,Jeong J,Cho BN,Eddy EM,Cho C

The human (PEDB) and mouse (mPEDB) Prostate Expression Databases.
Nucleic acids research30(1)218-20(2002 Jan)
Nelson PS,Pritchard C,Abbott D,Clegg N

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Spink2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報