Med6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med6
Entrez Gene ID	69792
Full Name	mediator complex subunit 6
Synonyms	1500012F11Rik,AU019660,AV213303
General protein information	Preferred Names mediator complex subunit 6 Names mediator of RNA polymerase II transcription subunit 6 mediator of RNA polymerase II transcription, subunit 6 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011244171.1	XP_011242473.1	mediator of RNA polymerase II transcription subunit 6 isoform X1
NM_001347385.1	NP_001334314.1	mediator of RNA polymerase II transcription subunit 6 isoform 2
NM_001347384.1	NP_001334313.1	mediator of RNA polymerase II transcription subunit 6 isoform 1
NM_027213.2	NP_081489.2	mediator of RNA polymerase II transcription subunit 6 isoform 4
NM_001347386.1	NP_001334315.1	mediator of RNA polymerase II transcription subunit 6 isoform 3

Pathways from BioSystems

Homologies

	MED6	XP_001082932.1
Drosophila melanogaster (fruit fly)	MED6	NP_731403.2
Pan troglodytes (chimpanzee)	MED6	XP_001146137.1
Bos taurus (cattle)	MED6	NP_001029409.1
Gallus gallus (chicken)	MED6	NP_001152897.1
Caenorhabditis elegans (roundworm)	mdt-6	NP_504791.3
Canis lupus familiaris (dog)	MED6	XP_005623650.1
Mus musculus (house mouse)	Med6	NP_081489.2
Rattus norvegicus (Norway rat)	Med6	NP_001100212.1
Arabidopsis thaliana (thale cress)	MED6	NP_188772.2
Xenopus tropicalis (tropical clawed frog)	med6	NP_001016292.1
Homo sapiens (human)	MED6	NP_001271138.1
Danio rerio (zebrafish)	med6	NP_001002084.1
Anopheles gambiae (African malaria mosquito)	MED6_ANOGA	XP_319180.3
Oryza sativa (rice)	Os06g0217500	NP_001057150.1

Gene Ontology
Bibliography

Related articles in PubMed

MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis.
Aranda-Orgilles B, Salda?a-Meyer R, Wang E, Trompouki E, Fassl A, Lau S, Mullenders J, Rocha PP, Raviram R, Guillamot M, S?nchez-D?az M, Wang K, Kayembe C, Zhang N, Amoasii L, Choudhuri A, Skok JA, Schober M, Reinberg D, Sicinski P, Schrewe H, Tsirigos A, Zon LI, Aifantis I
Cell stem cell19(6)784-799(2016 Dec)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Homologs of genes expressed in Caenorhabditis elegans GABAergic neurons are also found in the developing mouse forebrain.
Hammock EA, Eagleson KL, Barlow S, Earls LR, Miller DM 3rd, Levitt P
Neural development532(2010 Dec)

Mediator and cohesin connect gene expression and chromatin architecture.
Kagey MH, Newman JJ, Bilodeau S, Zhan Y, Orlando DA, van Berkum NL, Ebmeier CC, Goossens J, Rahl PB, Levine SS, Taatjes DJ, Dekker J, Young RA
Nature467(7314)430-5(2010 Sep)

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
Krebs AR, Demmers J, Karmodiya K, Chang NC, Chang AC, Tora L
EMBO reports11(7)541-7(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Med6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu48093	NM_001347384.1 Latest version!	Mus musculus mediator complex subunit 6 (Med6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu48094	NM_001347385.1 Latest version!	Mus musculus mediator complex subunit 6 (Med6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu03882	NM_027213.2 Latest version!	Mus musculus mediator complex subunit 6 (Med6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu03882	NM_001347386.1 Latest version!	Mus musculus mediator complex subunit 6 (Med6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu03882	XM_011244171.1 Latest version!	Mus musculus mediator complex subunit 6 (Med6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med6 ( NM_001347384.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334313.1 Mus musculus mediator complex subunit 6 (Med6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu48093
Clone ID Related Accession (Same CDS sequence)	NM_001347384.1
Accession Version	NM_001347384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154908.4, CK620521.1 and DV651747.1. On Nov 24, 2016 this sequence version replaced XM_006516211.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK005232.1, AK171784.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGGATATCCG AGATAACCTG CTGGGGATCT CCTGGGTTGA CAGCTCTTGG 
ATCCCCATCT TGAACAGTGG CAGTGTCCTG GATTACTTTT CAGAAAGAAG CAATCCTTTC 
TATGACAGGA CGTGTAATAA TGAGGTGGTC AAAATGCAGA GGCTGACATT AGAGCACTTG 
AATCAGATGG TTGGAATCGA ATACATCCTT TTACATGCTC AAGAGCCCAT TCTTTTCATC 
ATTCGAAAGC AACAGCGGCA GTCCCCTGCT CAAGTTATTC CACTGGCTGA TTACTATATC 
ATCGCTGGAG TCATCTATCA GGCGCCAGAC TTGGGATCAG TTATAAACTC CAGAGTGCTT 
ACTGCAGTGC ATGGAATTCA GTCAGCTTTT GATGAAGCTA TGTCATACTG TCGATACCAT 
CCTTCCAAAG GATATTGGTG GCACTTTAAA GATCACGAAG AACAAGAGAA AGTCAAACCT 
AAAGCCAAAA GGAAAGAAGA ACCAAGCTCT ATTTTTCAAA GACAGCGTGT GGATGCTTTA 
CTCATAGACC TTAGACAGAA ATTTCCACCC AGATTTGTTC AGCAAAAGTC TGGAGAAAAG 
CCTGTCCCAG TGGATCAGGC AAAAAAAGAG GCAGAGCCTT TGCCAGAAAC TGTAAAATCT 
GAGGAGAAGG AGAGCACAAA GAACATTCAG CAGACTGTGA GCACTAAAGG TCCCCCTGAG 
AAACGAATGA GACTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334313.1
CDS	48..788
Translation	MAAVDIRDNLLGISWVDSSWIPILNSGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQEKVKPKAKRKEEPSSIFQRQRVDALLIDLRQKFPPRFVQQKSGEKPVPVDQAKKEAEPLPETVKSEEKESTKNIQQTVSTKGPPEKRMRLQ

Target ORF information:

RefSeq Version	NM_001347384.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 6 (Med6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347384.1

ORF Insert Sequence:

ATGGCGGCGG TGGATATCCG AGATAACCTG CTGGGGATCT CCTGGGTTGA CAGCTCTTGG 
ATCCCCATCT TGAACAGTGG CAGTGTCCTG GATTACTTTT CAGAAAGAAG CAATCCTTTC 
TATGACAGGA CGTGTAATAA TGAGGTGGTC AAAATGCAGA GGCTGACATT AGAGCACTTG 
AATCAGATGG TTGGAATCGA ATACATCCTT TTACATGCTC AAGAGCCCAT TCTTTTCATC 
ATTCGAAAGC AACAGCGGCA GTCCCCTGCT CAAGTTATTC CACTGGCTGA TTACTATATC 
ATCGCTGGAG TCATCTATCA GGCGCCAGAC TTGGGATCAG TTATAAACTC CAGAGTGCTT 
ACTGCAGTGC ATGGAATTCA GTCAGCTTTT GATGAAGCTA TGTCATACTG TCGATACCAT 
CCTTCCAAAG GATATTGGTG GCACTTTAAA GATCACGAAG AACAAGAGAA AGTCAAACCT 
AAAGCCAAAA GGAAAGAAGA ACCAAGCTCT ATTTTTCAAA GACAGCGTGT GGATGCTTTA 
CTCATAGACC TTAGACAGAA ATTTCCACCC AGATTTGTTC AGCAAAAGTC TGGAGAAAAG 
CCTGTCCCAG TGGATCAGGC AAAAAAAGAG GCAGAGCCTT TGCCAGAAAC TGTAAAATCT 
GAGGAGAAGG AGAGCACAAA GAACATTCAG CAGACTGTGA GCACTAAAGG TCCCCCTGAG 
AAACGAATGA GACTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med6 ( NM_001347385.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334314.1 Mus musculus mediator complex subunit 6 (Med6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu48094
Clone ID Related Accession (Same CDS sequence)	NM_001347385.1
Accession Version	NM_001347385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154908.4, CK620521.1 and DV651747.1. On Nov 24, 2016 this sequence version replaced XM_006516213.3. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus], compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ085863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGGATATCCG AGATAACCTG CTGGGGATCT CCTGGGTTGA CAGCTCTTGG 
ATCCCCATCT TGAACAGTGG CAGTGTCCTG GATTACTTTT CAGAAAGAAG CAATCCTTTC 
TATGACAGGA CGTGTAATAA TGAGGTGGTC AAAATGCAGA GGCTGACATT AGAGCACTTG 
AATCAGATGG TTGGAATCGA ATACATCCTT TTACATGCTC AAGAGCCCAT TCTTTTCATC 
ATTCGAAAGC AACAGCGGCA GTCCCCTGCT CAAGTTATTC CACTGGCTGA TTACTATATC 
ATCGCTGGAG TCATCTATCA GGCGCCAGAC TTGGGATCAG TTATAAACTC CAGAGTGCTT 
ACTGCAGTGC ATGGAATTCA GTCAGCTTTT GATGAAGCTA TGTCATACTG TCGATACCAT 
CCTTCCAAAG GATATTGGTG GCACTTTAAA GATCACGAAG AACAAGCAAA AGTCTGGAGA 
AAAGCCTGTC CCAGTGGATC AGGCAAAAAA AGAGGCAGAG CCTTTGCCAG AAACTGTAAA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334314.1
CDS	48..593
Translation	MAAVDIRDNLLGISWVDSSWIPILNSGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQAKVWRKACPSGSGKKRGRAFARNCKI

Target ORF information:

RefSeq Version	NM_001347385.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 6 (Med6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347385.1

ORF Insert Sequence:

ATGGCGGCGG TGGATATCCG AGATAACCTG CTGGGGATCT CCTGGGTTGA CAGCTCTTGG 
ATCCCCATCT TGAACAGTGG CAGTGTCCTG GATTACTTTT CAGAAAGAAG CAATCCTTTC 
TATGACAGGA CGTGTAATAA TGAGGTGGTC AAAATGCAGA GGCTGACATT AGAGCACTTG 
AATCAGATGG TTGGAATCGA ATACATCCTT TTACATGCTC AAGAGCCCAT TCTTTTCATC 
ATTCGAAAGC AACAGCGGCA GTCCCCTGCT CAAGTTATTC CACTGGCTGA TTACTATATC 
ATCGCTGGAG TCATCTATCA GGCGCCAGAC TTGGGATCAG TTATAAACTC CAGAGTGCTT 
ACTGCAGTGC ATGGAATTCA GTCAGCTTTT GATGAAGCTA TGTCATACTG TCGATACCAT 
CCTTCCAAAG GATATTGGTG GCACTTTAAA GATCACGAAG AACAAGCAAA AGTCTGGAGA 
AAAGCCTGTC CCAGTGGATC AGGCAAAAAA AGAGGCAGAG CCTTTGCCAG AAACTGTAAA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med6 ( NM_027213.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081489.2 Mus musculus mediator complex subunit 6 (Med6), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu03882
Clone ID Related Accession (Same CDS sequence)	XM_011244171.1 , NM_027213.2 , NM_001347386.1
Accession Version	NM_027213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 6 isoform 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC013096.1. On Jan 15, 2003 this sequence version replaced NM_027213.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013096.1, AK005232.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAGGC TGACATTAGA GCACTTGAAT CAGATGGTTG GAATCGAATA CATCCTTTTA 
CATGCTCAAG AGCCCATTCT TTTCATCATT CGAAAGCAAC AGCGGCAGTC CCCTGCTCAA 
GTTATTCCAC TGGCTGATTA CTATATCATC GCTGGAGTCA TCTATCAGGC GCCAGACTTG 
GGATCAGTTA TAAACTCCAG AGTGCTTACT GCAGTGCATG GAATTCAGTC AGCTTTTGAT 
GAAGCTATGT CATACTGTCG ATACCATCCT TCCAAAGGAT ATTGGTGGCA CTTTAAAGAT 
CACGAAGAAC AAGAGAAAGT CAAACCTAAA GCCAAAAGGA AAGAAGAACC AAGCTCTATT 
TTTCAAAGAC AGCGTGTGGA TGCTTTACTC ATAGACCTTA GACAGAAATT TCCACCCAGA 
TTTGTTCAGC AAAAGTCTGG AGAAAAGCCT GTCCCAGTGG ATCAGGCAAA AAAAGAGGCA 
GAGCCTTTGC CAGAAACTGT AAAATCTGAG GAGAAGGAGA GCACAAAGAA CATTCAGCAG 
ACTGTGAGCA CTAAAGGTCC CCCTGAGAAA CGAATGAGAC TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081489.2
CDS	151..738
Translation	MQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQEKVKPKAKRKEEPSSIFQRQRVDALLIDLRQKFPPRFVQQKSGEKPVPVDQAKKEAEPLPETVKSEEKESTKNIQQTVSTKGPPEKRMRLQ

Target ORF information:

RefSeq Version	NM_027213.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 6 (Med6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027213.2

ORF Insert Sequence:

ATGCAGAGGC TGACATTAGA GCACTTGAAT CAGATGGTTG GAATCGAATA CATCCTTTTA 
CATGCTCAAG AGCCCATTCT TTTCATCATT CGAAAGCAAC AGCGGCAGTC CCCTGCTCAA 
GTTATTCCAC TGGCTGATTA CTATATCATC GCTGGAGTCA TCTATCAGGC GCCAGACTTG 
GGATCAGTTA TAAACTCCAG AGTGCTTACT GCAGTGCATG GAATTCAGTC AGCTTTTGAT 
GAAGCTATGT CATACTGTCG ATACCATCCT TCCAAAGGAT ATTGGTGGCA CTTTAAAGAT 
CACGAAGAAC AAGAGAAAGT CAAACCTAAA GCCAAAAGGA AAGAAGAACC AAGCTCTATT 
TTTCAAAGAC AGCGTGTGGA TGCTTTACTC ATAGACCTTA GACAGAAATT TCCACCCAGA 
TTTGTTCAGC AAAAGTCTGG AGAAAAGCCT GTCCCAGTGG ATCAGGCAAA AAAAGAGGCA 
GAGCCTTTGC CAGAAACTGT AAAATCTGAG GAGAAGGAGA GCACAAAGAA CATTCAGCAG 
ACTGTGAGCA CTAAAGGTCC CCCTGAGAAA CGAATGAGAC TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med6 ( NM_001347386.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334315.1 Mus musculus mediator complex subunit 6 (Med6), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu03882
Clone ID Related Accession (Same CDS sequence)	XM_011244171.1 , NM_027213.2 , NM_001347386.1
Accession Version	NM_001347386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 6 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154908.4, CK620521.1 and DV651747.1. On Nov 24, 2016 this sequence version replaced XM_017315178.1. Transcript Variant: This variant (3) contains an alternate exon in its 5' UTR compared to variant 1. This variant represents translation initiation at a downstream start codon compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning results in translation initiation at the downstream start codon to encode an isoform (3) that has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.414982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGGC TGACATTAGA GCACTTGAAT CAGATGGTTG GAATCGAATA CATCCTTTTA 
CATGCTCAAG AGCCCATTCT TTTCATCATT CGAAAGCAAC AGCGGCAGTC CCCTGCTCAA 
GTTATTCCAC TGGCTGATTA CTATATCATC GCTGGAGTCA TCTATCAGGC GCCAGACTTG 
GGATCAGTTA TAAACTCCAG AGTGCTTACT GCAGTGCATG GAATTCAGTC AGCTTTTGAT 
GAAGCTATGT CATACTGTCG ATACCATCCT TCCAAAGGAT ATTGGTGGCA CTTTAAAGAT 
CACGAAGAAC AAGAGAAAGT CAAACCTAAA GCCAAAAGGA AAGAAGAACC AAGCTCTATT 
TTTCAAAGAC AGCGTGTGGA TGCTTTACTC ATAGACCTTA GACAGAAATT TCCACCCAGA 
TTTGTTCAGC AAAAGTCTGG AGAAAAGCCT GTCCCAGTGG ATCAGGCAAA AAAAGAGGCA 
GAGCCTTTGC CAGAAACTGT AAAATCTGAG GAGAAGGAGA GCACAAAGAA CATTCAGCAG 
ACTGTGAGCA CTAAAGGTCC CCCTGAGAAA CGAATGAGAC TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334315.1
CDS	268..855
Translation	MQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQEKVKPKAKRKEEPSSIFQRQRVDALLIDLRQKFPPRFVQQKSGEKPVPVDQAKKEAEPLPETVKSEEKESTKNIQQTVSTKGPPEKRMRLQ

Target ORF information:

RefSeq Version	NM_001347386.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 6 (Med6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347386.1

ORF Insert Sequence:

ATGCAGAGGC TGACATTAGA GCACTTGAAT CAGATGGTTG GAATCGAATA CATCCTTTTA 
CATGCTCAAG AGCCCATTCT TTTCATCATT CGAAAGCAAC AGCGGCAGTC CCCTGCTCAA 
GTTATTCCAC TGGCTGATTA CTATATCATC GCTGGAGTCA TCTATCAGGC GCCAGACTTG 
GGATCAGTTA TAAACTCCAG AGTGCTTACT GCAGTGCATG GAATTCAGTC AGCTTTTGAT 
GAAGCTATGT CATACTGTCG ATACCATCCT TCCAAAGGAT ATTGGTGGCA CTTTAAAGAT 
CACGAAGAAC AAGAGAAAGT CAAACCTAAA GCCAAAAGGA AAGAAGAACC AAGCTCTATT 
TTTCAAAGAC AGCGTGTGGA TGCTTTACTC ATAGACCTTA GACAGAAATT TCCACCCAGA 
TTTGTTCAGC AAAAGTCTGG AGAAAAGCCT GTCCCAGTGG ATCAGGCAAA AAAAGAGGCA 
GAGCCTTTGC CAGAAACTGT AAAATCTGAG GAGAAGGAGA GCACAAAGAA CATTCAGCAG 
ACTGTGAGCA CTAAAGGTCC CCCTGAGAAA CGAATGAGAC TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med6 ( XM_011244171.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242473.1 Mus musculus mediator complex subunit 6 (Med6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu03882
Clone ID Related Accession (Same CDS sequence)	XM_011244171.1 , NM_027213.2 , NM_001347386.1
Accession Version	XM_011244171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	mediator of RNA polymerase II transcription subunit 6 isoform X1
Comment

ATGCAGAGGC TGACATTAGA GCACTTGAAT CAGATGGTTG GAATCGAATA CATCCTTTTA 
CATGCTCAAG AGCCCATTCT TTTCATCATT CGAAAGCAAC AGCGGCAGTC CCCTGCTCAA 
GTTATTCCAC TGGCTGATTA CTATATCATC GCTGGAGTCA TCTATCAGGC GCCAGACTTG 
GGATCAGTTA TAAACTCCAG AGTGCTTACT GCAGTGCATG GAATTCAGTC AGCTTTTGAT 
GAAGCTATGT CATACTGTCG ATACCATCCT TCCAAAGGAT ATTGGTGGCA CTTTAAAGAT 
CACGAAGAAC AAGAGAAAGT CAAACCTAAA GCCAAAAGGA AAGAAGAACC AAGCTCTATT 
TTTCAAAGAC AGCGTGTGGA TGCTTTACTC ATAGACCTTA GACAGAAATT TCCACCCAGA 
TTTGTTCAGC AAAAGTCTGG AGAAAAGCCT GTCCCAGTGG ATCAGGCAAA AAAAGAGGCA 
GAGCCTTTGC CAGAAACTGT AAAATCTGAG GAGAAGGAGA GCACAAAGAA CATTCAGCAG 
ACTGTGAGCA CTAAAGGTCC CCCTGAGAAA CGAATGAGAC TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242473.1
Translation	MQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQEKVKPKAKRKEEPSSIFQRQRVDALLIDLRQKFPPRFVQQKSGEKPVPVDQAKKEAEPLPETVKSEEKESTKNIQQTVSTKGPPEKRMRLQ

Target ORF information:

RefSeq Version	XM_011244171.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus mediator complex subunit 6 (Med6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244171.1

ORF Insert Sequence:

ATGCAGAGGC TGACATTAGA GCACTTGAAT CAGATGGTTG GAATCGAATA CATCCTTTTA 
CATGCTCAAG AGCCCATTCT TTTCATCATT CGAAAGCAAC AGCGGCAGTC CCCTGCTCAA 
GTTATTCCAC TGGCTGATTA CTATATCATC GCTGGAGTCA TCTATCAGGC GCCAGACTTG 
GGATCAGTTA TAAACTCCAG AGTGCTTACT GCAGTGCATG GAATTCAGTC AGCTTTTGAT 
GAAGCTATGT CATACTGTCG ATACCATCCT TCCAAAGGAT ATTGGTGGCA CTTTAAAGAT 
CACGAAGAAC AAGAGAAAGT CAAACCTAAA GCCAAAAGGA AAGAAGAACC AAGCTCTATT 
TTTCAAAGAC AGCGTGTGGA TGCTTTACTC ATAGACCTTA GACAGAAATT TCCACCCAGA 
TTTGTTCAGC AAAAGTCTGG AGAAAAGCCT GTCCCAGTGG ATCAGGCAAA AAAAGAGGCA 
GAGCCTTTGC CAGAAACTGT AAAATCTGAG GAGAAGGAGA GCACAAAGAA CATTCAGCAG 
ACTGTGAGCA CTAAAGGTCC CCCTGAGAAA CGAATGAGAC TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis.
Cell stem cell19(6)784-799(2016 Dec)
Aranda-Orgilles B,Salda?a-Meyer R,Wang E,Trompouki E,Fassl A,Lau S,Mullenders J,Rocha PP,Raviram R,Guillamot M,S?nchez-D?az M,Wang K,Kayembe C,Zhang N,Amoasii L,Choudhuri A,Skok JA,Schober M,Reinberg D,Sicinski P,Schrewe H,Tsirigos A,Zon LI,Aifantis I

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Homologs of genes expressed in Caenorhabditis elegans GABAergic neurons are also found in the developing mouse forebrain.
Neural development532(2010 Dec)
Hammock EA,Eagleson KL,Barlow S,Earls LR,Miller DM 3rd,Levitt P

Mediator and cohesin connect gene expression and chromatin architecture.
Nature467(7314)430-5(2010 Sep)
Kagey MH,Newman JJ,Bilodeau S,Zhan Y,Orlando DA,van Berkum NL,Ebmeier CC,Goossens J,Rahl PB,Levine SS,Taatjes DJ,Dekker J,Young RA

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
EMBO reports11(7)541-7(2010 Jul)
Krebs AR,Demmers J,Karmodiya K,Chang NC,Chang AC,Tora L

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Med6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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