Smap2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smap2
Entrez Gene ID	69780
Full Name	small ArfGAP 2
Synonyms	1810031K02Rik,AA408614,Smap1l
General protein information	Preferred Names small ArfGAP 2 Names stromal membrane-associated protein 2 stromal membrane-associated GTPase-activating protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006503378.3	XP_006503441.1	stromal membrane-associated protein 2 isoform X1
XM_006503379.3	XP_006503442.1	stromal membrane-associated protein 2 isoform X2
XM_006503380.2	XP_006503443.1	stromal membrane-associated protein 2 isoform X3
XM_006503378.2	XP_006503441.1	stromal membrane-associated protein 2 isoform X1
XM_006503379.4	XP_006503442.1	stromal membrane-associated protein 2 isoform X2
NM_133716.3	NP_598477.2	stromal membrane-associated protein 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SMAP2	NP_073570.1
Bos taurus (cattle)	SMAP2	NP_001069138.1
Gallus gallus (chicken)	SMAP2	NP_001026073.1
Drosophila melanogaster (fruit fly)	CG8243	NP_610424.1
	SMAP2	XP_001083189.2
Canis lupus familiaris (dog)	SMAP2	XP_005628934.1
Mus musculus (house mouse)	Smap2	NP_598477.2
Danio rerio (zebrafish)	smap2	NP_001038260.1
Pan troglodytes (chimpanzee)	SMAP2	XP_513355.3
Rattus norvegicus (Norway rat)	Smap2	NP_001094139.1
Xenopus tropicalis (tropical clawed frog)	smap2	XP_004911663.1

Gene Ontology
Bibliography

Related articles in PubMed

Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality.
Sumiyoshi M, Masuda N, Tanuma N, Ogoh H, Imai E, Otsuka M, Hayakawa N, Ohno K, Matsui Y, Hara K, Gotoh R, Suzuki M, Rai S, Tanaka H, Matsumura I, Shima H, Watanabe T
FEBS letters589(19 Pt B)2754-62(2015 Sep)

The Arf GAP SMAP2 is necessary for organized vesicle budding from the trans-Golgi network and subsequent acrosome formation in spermiogenesis.
Funaki T, Kon S, Tanabe K, Natsume W, Sato S, Shimizu T, Yoshida N, Wong WF, Ogura A, Ogawa T, Inoue K, Ogonuki N, Miki H, Mochida K, Endoh K, Yomogida K, Fukumoto M, Horai R, Iwakura Y, Ito C, Toshimori K, Watanabe T, Satake M
Molecular biology of the cell24(17)2633-44(2013 Sep)

Genomic strategy identifies a missense mutation in WD-repeat domain 65 (WDR65) in an individual with Van der Woude syndrome.
Rorick NK, Kinoshita A, Weirather JL, Peyrard-Janvid M, de Lima RL, Dunnwald M, Shanske AL, Moretti-Ferreira D, Koillinen H, Kere J, Mansilla MA, Murray JC, Goudy SL, Schutte BC
American journal of medical genetics. Part A155A(6)1314-21(2011 Jun)

Localization of SMAP2 to the TGN and its function in the regulation of TGN protein transport.
Funaki T, Kon S, Ronn RE, Henmi Y, Kobayashi Y, Watanabe T, Nakayama K, Tanabe K, Satake M
Cell structure and function36(1)83-95(2011)

SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network.
Natsume W, Tanabe K, Kon S, Yoshida N, Watanabe T, Torii T, Satake M
Molecular biology of the cell17(6)2592-603(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

either SMAP1 or SMAP2, is required for proper embryogenesis
Title: Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality.

study reveals a link between SMAP2 and CALM/syntaxin2 in clathrin-coated vesicle formation from the trans-Golgi network and subsequent acrosome formation
Title: The Arf GAP SMAP2 is necessary for organized vesicle budding from the trans-Golgi network and subsequent acrosome formation in spermiogenesis.

the trans-Golgi network (TGN) was verified as an additional site of SMAP2 localization based on its co-localization with various TGN-marker proteins.
Title: Localization of SMAP2 to the TGN and its function in the regulation of TGN protein transport.

The following Smap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu33802	XM_006503380.2 Latest version!	Mus musculus small ArfGAP 2 (Smap2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu15417	NM_133716.3 Latest version!	Mus musculus small ArfGAP 2 (Smap2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu33800	XM_006503378.3 Latest version!	Mus musculus small ArfGAP 2 (Smap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu33801	XM_006503379.4 Latest version!	Mus musculus small ArfGAP 2 (Smap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu33800	XM_006503378.2	Mus musculus small ArfGAP 2 (Smap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu33801	XM_006503379.3	Mus musculus small ArfGAP 2 (Smap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smap2 ( XM_006503378.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503441.1 Mus musculus small ArfGAP 2 (Smap2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33800
Clone ID Related Accession (Same CDS sequence)	XM_006503378.3 , XM_006503378.2
Accession Version	XM_006503378.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	stromal membrane-associated protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA TGGGGAATGG AAAAGCAAAC CGACTCTATG AAGCCTACCT TCCCGAGACC 
TTTCGGCGAC CTCAGATAGA CCCAGCTGTT GAAGGATTTA TTCGAGATAA ATATGAGAAG 
AAGAAATATA TGGACCGAAG TCTGGACATC AATGTCCTTA GGAAAGAGAA GGATGATAAG 
TGGAAACGAG GGAATGAACC CGCTCCGGAG AAAAAGATGG AACCTGTTGT TTTTGAGAAA 
GTAAAAATGC CACAGAAAAA AGAAGACGCA CAGCTACCTC GGAAAAGCTC TCCGAAATCT 
GCAGCCCCTG TCATGGACTT GTTGGGCCTT GATGCTCCTG TGGCCTGCTC AATTGCAAAC 
AGTAAGACCA GCAATGCCCT AGAAAAGGAT CTAGATCTTT TGGCCTCTGT TCCGTCCCCT 
TCTTCAGTTT CCAGAAAGGC TGTAGGTTCC ATGCCAACTG CAGGGAGTGC TGGTTCTGTC 
CCTGAAAACC TGAACCTATT TCCAGAGCCA GGGAGCAAAT CAGAAGAAAC AGGCAAGAAA 
CAGCTCTCCA AGGACTCCAT CCTATCACTG TATGGATCCC AGACGCCTCA AATGCCTGCC 
CAAGCAATGT TCATGGCTCC TGCTCAGATG GCATACCCCA CAGCTTACCC CAGTTTCCCC 
GGGGTTACAC CACCTAACAG CATCATGGGG GGCATGGTGC CTCCGCCAGT AGGCATGGTA 
GCTCAACCGG GAGCCTCTGG AATGCTAACC CCTATGGCCA TGCCTGCAGG CTATATGGGG 
GGCATGCAGG CGTCCATGAT GGGTGTGCCG AACGGGATGA TGACCACTCA GCAGGCCGGC 
TACATGGCAA GCATGGCAGC TATGCCGCAG ACTGTGTATG GAGTTCAGCC AGCTCAGCAA 
CTGCAATGGA ACCTTACTCA GATGACCCAG CAAATGGCTG GGATGAACTT CTACGGAGCC 
AACGGCATGA TGAACTATGG ACAGTCAATG GGCGGTGGAA ATGGCCAGGC AGCCAATCAG 
ACTCTCAGTC CTCAGATGTG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503441.1
CDS	83..1129
Translation	MQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINVLRKEKDDKWKRGNEPAPEKKMEPVVFEKVKMPQKKEDAQLPRKSSPKSAAPVMDLLGLDAPVACSIANSKTSNALEKDLDLLASVPSPSSVSRKAVGSMPTAGSAGSVPENLNLFPEPGSKSEETGKKQLSKDSILSLYGSQTPQMPAQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGGMVPPPVGMVAQPGASGMLTPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMASMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMGGGNGQAANQTLSPQMWK

Target ORF information:

RefSeq Version	XM_006503378.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ArfGAP 2 (Smap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503378.2

ORF Insert Sequence:

ATGCAAGAGA TGGGGAATGG AAAAGCAAAC CGACTCTATG AAGCCTACCT TCCCGAGACC 
TTTCGGCGAC CTCAGATAGA CCCAGCTGTT GAAGGATTTA TTCGAGATAA ATATGAGAAG 
AAGAAATATA TGGACCGAAG TCTGGACATC AATGTCCTTA GGAAAGAGAA GGATGATAAG 
TGGAAACGAG GGAATGAACC CGCTCCGGAG AAAAAGATGG AACCTGTTGT TTTTGAGAAA 
GTAAAAATGC CACAGAAAAA AGAAGACGCA CAGCTACCTC GGAAAAGCTC TCCGAAATCT 
GCAGCCCCTG TCATGGACTT GTTGGGCCTT GATGCTCCTG TGGCCTGCTC AATTGCAAAC 
AGTAAGACCA GCAATGCCCT AGAAAAGGAT CTAGATCTTT TGGCCTCTGT TCCGTCCCCT 
TCTTCAGTTT CCAGAAAGGC TGTAGGTTCC ATGCCAACTG CAGGGAGTGC TGGTTCTGTC 
CCTGAAAACC TGAACCTATT TCCAGAGCCA GGGAGCAAAT CAGAAGAAAC AGGCAAGAAA 
CAGCTCTCCA AGGACTCCAT CCTATCACTG TATGGATCCC AGACGCCTCA AATGCCTGCC 
CAAGCAATGT TCATGGCTCC TGCTCAGATG GCATACCCCA CAGCTTACCC CAGTTTCCCC 
GGGGTTACAC CACCTAACAG CATCATGGGG GGCATGGTGC CTCCGCCAGT AGGCATGGTA 
GCTCAACCGG GAGCCTCTGG AATGCTAACC CCTATGGCCA TGCCTGCAGG CTATATGGGG 
GGCATGCAGG CGTCCATGAT GGGTGTGCCG AACGGGATGA TGACCACTCA GCAGGCCGGC 
TACATGGCAA GCATGGCAGC TATGCCGCAG ACTGTGTATG GAGTTCAGCC AGCTCAGCAA 
CTGCAATGGA ACCTTACTCA GATGACCCAG CAAATGGCTG GGATGAACTT CTACGGAGCC 
AACGGCATGA TGAACTATGG ACAGTCAATG GGCGGTGGAA ATGGCCAGGC AGCCAATCAG 
ACTCTCAGTC CTCAGATGTG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smap2 ( XM_006503379.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503442.1 Mus musculus small ArfGAP 2 (Smap2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu33801
Clone ID Related Accession (Same CDS sequence)	XM_006503379.3 , XM_006503379.4
Accession Version	XM_006503379.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	stromal membrane-associated protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503379.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTACT GTGCTCTATT GAACCCTGGC AATTGGCAAA AGTGGCACTG CTTTGTAAAT 
ATCTTGTATA AAAGTATTGA CTTTGACTGG TTTATCAATA TTGCTCTTCC TCCATTCCCT 
CAGCCCGGGC TGACAGACAC AGAGCAAGCC CATTTGAAGA AAGAGAAGGA TGATAAGTGG 
AAACGAGGGA ATGAACCCGC TCCGGAGAAA AAGATGGAAC CTGTTGTTTT TGAGAAAGTA 
AAAATGCCAC AGAAAAAAGA AGACGCACAG CTACCTCGGA AAAGCTCTCC GAAATCTGCA 
GCCCCTGTCA TGGACTTGTT GGGCCTTGAT GCTCCTGTGG CCTGCTCAAT TGCAAACAGT 
AAGACCAGCA ATGCCCTAGA AAAGGATCTA GATCTTTTGG CCTCTGTTCC GTCCCCTTCT 
TCAGTTTCCA GAAAGGCTGT AGGTTCCATG CCAACTGCAG GGAGTGCTGG TTCTGTCCCT 
GAAAACCTGA ACCTATTTCC AGAGCCAGGG AGCAAATCAG AAGAAACAGG CAAGAAACAG 
CTCTCCAAGG ACTCCATCCT ATCACTGTAT GGATCCCAGA CGCCTCAAAT GCCTGCCCAA 
GCAATGTTCA TGGCTCCTGC TCAGATGGCA TACCCCACAG CTTACCCCAG TTTCCCCGGG 
GTTACACCAC CTAACAGCAT CATGGGGGGC ATGGTGCCTC CGCCAGTAGG CATGGTAGCT 
CAACCGGGAG CCTCTGGAAT GCTAACCCCT ATGGCCATGC CTGCAGGCTA TATGGGGGGC 
ATGCAGGCGT CCATGATGGG TGTGCCGAAC GGGATGATGA CCACTCAGCA GGCCGGCTAC 
ATGGCAAGCA TGGCAGCTAT GCCGCAGACT GTGTATGGAG TTCAGCCAGC TCAGCAACTG 
CAATGGAACC TTACTCAGAT GACCCAGCAA ATGGCTGGGA TGAACTTCTA CGGAGCCAAC 
GGCATGATGA ACTATGGACA GTCAATGGGC GGTGGAAATG GCCAGGCAGC CAATCAGACT 
CTCAGTCCTC AGATGTGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503442.1
CDS	263..1306
Translation	MAYCALLNPGNWQKWHCFVNILYKSIDFDWFINIALPPFPQPGLTDTEQAHLKKEKDDKWKRGNEPAPEKKMEPVVFEKVKMPQKKEDAQLPRKSSPKSAAPVMDLLGLDAPVACSIANSKTSNALEKDLDLLASVPSPSSVSRKAVGSMPTAGSAGSVPENLNLFPEPGSKSEETGKKQLSKDSILSLYGSQTPQMPAQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGGMVPPPVGMVAQPGASGMLTPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMASMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMGGGNGQAANQTLSPQMWK

Target ORF information:

RefSeq Version	XM_006503379.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ArfGAP 2 (Smap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503379.3

ORF Insert Sequence:

ATGGCTTACT GTGCTCTATT GAACCCTGGC AATTGGCAAA AGTGGCACTG CTTTGTAAAT 
ATCTTGTATA AAAGTATTGA CTTTGACTGG TTTATCAATA TTGCTCTTCC TCCATTCCCT 
CAGCCCGGGC TGACAGACAC AGAGCAAGCC CATTTGAAGA AAGAGAAGGA TGATAAGTGG 
AAACGAGGGA ATGAACCCGC TCCGGAGAAA AAGATGGAAC CTGTTGTTTT TGAGAAAGTA 
AAAATGCCAC AGAAAAAAGA AGACGCACAG CTACCTCGGA AAAGCTCTCC GAAATCTGCA 
GCCCCTGTCA TGGACTTGTT GGGCCTTGAT GCTCCTGTGG CCTGCTCAAT TGCAAACAGT 
AAGACCAGCA ATGCCCTAGA AAAGGATCTA GATCTTTTGG CCTCTGTTCC GTCCCCTTCT 
TCAGTTTCCA GAAAGGCTGT AGGTTCCATG CCAACTGCAG GGAGTGCTGG TTCTGTCCCT 
GAAAACCTGA ACCTATTTCC AGAGCCAGGG AGCAAATCAG AAGAAACAGG CAAGAAACAG 
CTCTCCAAGG ACTCCATCCT ATCACTGTAT GGATCCCAGA CGCCTCAAAT GCCTGCCCAA 
GCAATGTTCA TGGCTCCTGC TCAGATGGCA TACCCCACAG CTTACCCCAG TTTCCCCGGG 
GTTACACCAC CTAACAGCAT CATGGGGGGC ATGGTGCCTC CGCCAGTAGG CATGGTAGCT 
CAACCGGGAG CCTCTGGAAT GCTAACCCCT ATGGCCATGC CTGCAGGCTA TATGGGGGGC 
ATGCAGGCGT CCATGATGGG TGTGCCGAAC GGGATGATGA CCACTCAGCA GGCCGGCTAC 
ATGGCAAGCA TGGCAGCTAT GCCGCAGACT GTGTATGGAG TTCAGCCAGC TCAGCAACTG 
CAATGGAACC TTACTCAGAT GACCCAGCAA ATGGCTGGGA TGAACTTCTA CGGAGCCAAC 
GGCATGATGA ACTATGGACA GTCAATGGGC GGTGGAAATG GCCAGGCAGC CAATCAGACT 
CTCAGTCCTC AGATGTGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smap2 ( XM_006503380.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503443.1 Mus musculus small ArfGAP 2 (Smap2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu33802
Clone ID Related Accession (Same CDS sequence)	XM_006503380.2
Accession Version	XM_006503380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	stromal membrane-associated protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006503380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGCA AGTCGGTGAA GGACGTGGAT CGGTACCAGG CGGTCCTGGC CAACCTGCTG 
CTGGAGGAAG ATAACAAGTT CTGTGCTGAC TGCCAGTCCA AAGGGCCGAG ATGGGCCTCC 
TGGAACATCG GGGTGTTCAT CTGCATTCGG TGTGCTGGGA TCCACAGGAA TTTGGGGGTG 
CATATATCCA GGGTAAAATC AGTGAACCTC GACCAGTGGA CTCAAGAACA GATTCAGTGC 
ATGCAAGAGA TGGGGAATGG AAAAGCAAAC CGACTCTATG AAGCCTACCT TCCCGAGACC 
TTTCGGCGAC CTCAGATAGA CCCAGCTGTT GAAGGATTTA TTCGAGATAA ATATGAGAAG 
AAGAAATATA TGGACCGAAG TCTGGACATC AATGTCCTTA GGAAAGAGAA GGATGATAAG 
TGGAAACGAG GGAATGAACC CGCTCCGGAG AAAAAGATGG AACCTGTTGT TTTTGAGAAA 
GTAAAAATGC CACAGAAAAA AGAAGACGCA CAGCTACCTC GGAAAAGCTC TCCGAAATCT 
GCAGCCCCTG TCATGGACTT GTTGGGCCTT GATGCTCCTG TGGCCTGCTC AATTGCAAAC 
AGTAAGACCA GCAATGCCCT AGAAAAGGAT CTAGATCTTT TGGCCTCTGT TCCGTCCCCT 
TCTTCAGTTT CCAGAAAGGC TGTAGGTTCC ATGCTGACTG GTTCTCCTGT TGCCCTCTGT 
GCAGTAGGCT GTGGGTTCCA TGCTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503443.1
CDS	439..1188
Translation	MTGKSVKDVDRYQAVLANLLLEEDNKFCADCQSKGPRWASWNIGVFICIRCAGIHRNLGVHISRVKSVNLDQWTQEQIQCMQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINVLRKEKDDKWKRGNEPAPEKKMEPVVFEKVKMPQKKEDAQLPRKSSPKSAAPVMDLLGLDAPVACSIANSKTSNALEKDLDLLASVPSPSSVSRKAVGSMLTGSPVALCAVGCGFHAD

Target ORF information:

RefSeq Version	XM_006503380.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ArfGAP 2 (Smap2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503380.2

ORF Insert Sequence:

ATGACAGGCA AGTCGGTGAA GGACGTGGAT CGGTACCAGG CGGTCCTGGC CAACCTGCTG 
CTGGAGGAAG ATAACAAGTT CTGTGCTGAC TGCCAGTCCA AAGGGCCGAG ATGGGCCTCC 
TGGAACATCG GGGTGTTCAT CTGCATTCGG TGTGCTGGGA TCCACAGGAA TTTGGGGGTG 
CATATATCCA GGGTAAAATC AGTGAACCTC GACCAGTGGA CTCAAGAACA GATTCAGTGC 
ATGCAAGAGA TGGGGAATGG AAAAGCAAAC CGACTCTATG AAGCCTACCT TCCCGAGACC 
TTTCGGCGAC CTCAGATAGA CCCAGCTGTT GAAGGATTTA TTCGAGATAA ATATGAGAAG 
AAGAAATATA TGGACCGAAG TCTGGACATC AATGTCCTTA GGAAAGAGAA GGATGATAAG 
TGGAAACGAG GGAATGAACC CGCTCCGGAG AAAAAGATGG AACCTGTTGT TTTTGAGAAA 
GTAAAAATGC CACAGAAAAA AGAAGACGCA CAGCTACCTC GGAAAAGCTC TCCGAAATCT 
GCAGCCCCTG TCATGGACTT GTTGGGCCTT GATGCTCCTG TGGCCTGCTC AATTGCAAAC 
AGTAAGACCA GCAATGCCCT AGAAAAGGAT CTAGATCTTT TGGCCTCTGT TCCGTCCCCT 
TCTTCAGTTT CCAGAAAGGC TGTAGGTTCC ATGCTGACTG GTTCTCCTGT TGCCCTCTGT 
GCAGTAGGCT GTGGGTTCCA TGCTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smap2 ( NM_133716.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598477.2 Mus musculus small ArfGAP 2 (Smap2), mRNA.

Price & Availability↑

CloneID	OMu15417
Clone ID Related Accession (Same CDS sequence)	NM_133716.3
Accession Version	NM_133716.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	stromal membrane-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052413.1, BM944792.1 and BQ175680.1. On May 9, 2009 this sequence version replaced NM_133716.2. ##Evidence-Data-START## Transcript exon combination :: BC052413.1, BC031581.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGGCA AGTCGGTGAA GGACGTGGAT CGGTACCAGG CGGTCCTGGC CAACCTGCTG 
CTGGAGGAAG ATAACAAGTT CTGTGCTGAC TGCCAGTCCA AAGGGCCGAG ATGGGCCTCC 
TGGAACATCG GGGTGTTCAT CTGCATTCGG TGTGCTGGGA TCCACAGGAA TTTGGGGGTG 
CATATATCCA GGGTAAAATC AGTGAACCTC GACCAGTGGA CTCAAGAACA GATTCAGTGC 
ATGCAAGAGA TGGGGAATGG AAAAGCAAAC CGACTCTATG AAGCCTACCT TCCCGAGACC 
TTTCGGCGAC CTCAGATAGA CCCAGCTGTT GAAGGATTTA TTCGAGATAA ATATGAGAAG 
AAGAAATATA TGGACCGAAG TCTGGACATC AATGTCCTTA GGAAAGAGAA GGATGATAAG 
TGGAAACGAG GGAATGAACC CGCTCCGGAG AAAAAGATGG AACCTGTTGT TTTTGAGAAA 
GTAAAAATGC CACAGAAAAA AGAAGACGCA CAGCTACCTC GGAAAAGCTC TCCGAAATCT 
GCAGCCCCTG TCATGGACTT GTTGGGCCTT GATGCTCCTG TGGCCTGCTC AATTGCAAAC 
AGTAAGACCA GCAATGCCCT AGAAAAGGAT CTAGATCTTT TGGCCTCTGT TCCGTCCCCT 
TCTTCAGTTT CCAGAAAGGC TGTAGGTTCC ATGCCAACTG CAGGGAGTGC TGGTTCTGTC 
CCTGAAAACC TGAACCTATT TCCAGAGCCA GGGAGCAAAT CAGAAGAAAC AGGCAAGAAA 
CAGCTCTCCA AGGACTCCAT CCTATCACTG TATGGATCCC AGACGCCTCA AATGCCTGCC 
CAAGCAATGT TCATGGCTCC TGCTCAGATG GCATACCCCA CAGCTTACCC CAGTTTCCCC 
GGGGTTACAC CACCTAACAG CATCATGGGG GGCATGGTGC CTCCGCCAGT AGGCATGGTA 
GCTCAACCGG GAGCCTCTGG AATGCTAACC CCTATGGCCA TGCCTGCAGG CTATATGGGG 
GGCATGCAGG CGTCCATGAT GGGTGTGCCG AACGGGATGA TGACCACTCA GCAGGCCGGC 
TACATGGCAA GCATGGCAGC TATGCCGCAG ACTGTGTATG GAGTTCAGCC AGCTCAGCAA 
CTGCAATGGA ACCTTACTCA GATGACCCAG CAAATGGCTG GGATGAACTT CTACGGAGCC 
AACGGCATGA TGAACTATGG ACAGTCAATG GGCGGTGGAA ATGGCCAGGC AGCCAATCAG 
ACTCTCAGTC CTCAGATGTG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598477.2
CDS	397..1683
Translation	MTGKSVKDVDRYQAVLANLLLEEDNKFCADCQSKGPRWASWNIGVFICIRCAGIHRNLGVHISRVKSVNLDQWTQEQIQCMQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINVLRKEKDDKWKRGNEPAPEKKMEPVVFEKVKMPQKKEDAQLPRKSSPKSAAPVMDLLGLDAPVACSIANSKTSNALEKDLDLLASVPSPSSVSRKAVGSMPTAGSAGSVPENLNLFPEPGSKSEETGKKQLSKDSILSLYGSQTPQMPAQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGGMVPPPVGMVAQPGASGMLTPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMASMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMGGGNGQAANQTLSPQMWK

Target ORF information:

RefSeq Version	NM_133716.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ArfGAP 2 (Smap2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133716.3

ORF Insert Sequence:

ATGACAGGCA AGTCGGTGAA GGACGTGGAT CGGTACCAGG CGGTCCTGGC CAACCTGCTG 
CTGGAGGAAG ATAACAAGTT CTGTGCTGAC TGCCAGTCCA AAGGGCCGAG ATGGGCCTCC 
TGGAACATCG GGGTGTTCAT CTGCATTCGG TGTGCTGGGA TCCACAGGAA TTTGGGGGTG 
CATATATCCA GGGTAAAATC AGTGAACCTC GACCAGTGGA CTCAAGAACA GATTCAGTGC 
ATGCAAGAGA TGGGGAATGG AAAAGCAAAC CGACTCTATG AAGCCTACCT TCCCGAGACC 
TTTCGGCGAC CTCAGATAGA CCCAGCTGTT GAAGGATTTA TTCGAGATAA ATATGAGAAG 
AAGAAATATA TGGACCGAAG TCTGGACATC AATGTCCTTA GGAAAGAGAA GGATGATAAG 
TGGAAACGAG GGAATGAACC CGCTCCGGAG AAAAAGATGG AACCTGTTGT TTTTGAGAAA 
GTAAAAATGC CACAGAAAAA AGAAGACGCA CAGCTACCTC GGAAAAGCTC TCCGAAATCT 
GCAGCCCCTG TCATGGACTT GTTGGGCCTT GATGCTCCTG TGGCCTGCTC AATTGCAAAC 
AGTAAGACCA GCAATGCCCT AGAAAAGGAT CTAGATCTTT TGGCCTCTGT TCCGTCCCCT 
TCTTCAGTTT CCAGAAAGGC TGTAGGTTCC ATGCCAACTG CAGGGAGTGC TGGTTCTGTC 
CCTGAAAACC TGAACCTATT TCCAGAGCCA GGGAGCAAAT CAGAAGAAAC AGGCAAGAAA 
CAGCTCTCCA AGGACTCCAT CCTATCACTG TATGGATCCC AGACGCCTCA AATGCCTGCC 
CAAGCAATGT TCATGGCTCC TGCTCAGATG GCATACCCCA CAGCTTACCC CAGTTTCCCC 
GGGGTTACAC CACCTAACAG CATCATGGGG GGCATGGTGC CTCCGCCAGT AGGCATGGTA 
GCTCAACCGG GAGCCTCTGG AATGCTAACC CCTATGGCCA TGCCTGCAGG CTATATGGGG 
GGCATGCAGG CGTCCATGAT GGGTGTGCCG AACGGGATGA TGACCACTCA GCAGGCCGGC 
TACATGGCAA GCATGGCAGC TATGCCGCAG ACTGTGTATG GAGTTCAGCC AGCTCAGCAA 
CTGCAATGGA ACCTTACTCA GATGACCCAG CAAATGGCTG GGATGAACTT CTACGGAGCC 
AACGGCATGA TGAACTATGG ACAGTCAATG GGCGGTGGAA ATGGCCAGGC AGCCAATCAG 
ACTCTCAGTC CTCAGATGTG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smap2 ( XM_006503378.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503441.1 Mus musculus small ArfGAP 2 (Smap2), transcript variant X1, mRNA.

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CloneID	OMu33800
Clone ID Related Accession (Same CDS sequence)	XM_006503378.3 , XM_006503378.2
Accession Version	XM_006503378.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	stromal membrane-associated protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA TGGGGAATGG AAAAGCAAAC CGACTCTATG AAGCCTACCT TCCCGAGACC 
TTTCGGCGAC CTCAGATAGA CCCAGCTGTT GAAGGATTTA TTCGAGATAA ATATGAGAAG 
AAGAAATATA TGGACCGAAG TCTGGACATC AATGTCCTTA GGAAAGAGAA GGATGATAAG 
TGGAAACGAG GGAATGAACC CGCTCCGGAG AAAAAGATGG AACCTGTTGT TTTTGAGAAA 
GTAAAAATGC CACAGAAAAA AGAAGACGCA CAGCTACCTC GGAAAAGCTC TCCGAAATCT 
GCAGCCCCTG TCATGGACTT GTTGGGCCTT GATGCTCCTG TGGCCTGCTC AATTGCAAAC 
AGTAAGACCA GCAATGCCCT AGAAAAGGAT CTAGATCTTT TGGCCTCTGT TCCGTCCCCT 
TCTTCAGTTT CCAGAAAGGC TGTAGGTTCC ATGCCAACTG CAGGGAGTGC TGGTTCTGTC 
CCTGAAAACC TGAACCTATT TCCAGAGCCA GGGAGCAAAT CAGAAGAAAC AGGCAAGAAA 
CAGCTCTCCA AGGACTCCAT CCTATCACTG TATGGATCCC AGACGCCTCA AATGCCTGCC 
CAAGCAATGT TCATGGCTCC TGCTCAGATG GCATACCCCA CAGCTTACCC CAGTTTCCCC 
GGGGTTACAC CACCTAACAG CATCATGGGG GGCATGGTGC CTCCGCCAGT AGGCATGGTA 
GCTCAACCGG GAGCCTCTGG AATGCTAACC CCTATGGCCA TGCCTGCAGG CTATATGGGG 
GGCATGCAGG CGTCCATGAT GGGTGTGCCG AACGGGATGA TGACCACTCA GCAGGCCGGC 
TACATGGCAA GCATGGCAGC TATGCCGCAG ACTGTGTATG GAGTTCAGCC AGCTCAGCAA 
CTGCAATGGA ACCTTACTCA GATGACCCAG CAAATGGCTG GGATGAACTT CTACGGAGCC 
AACGGCATGA TGAACTATGG ACAGTCAATG GGCGGTGGAA ATGGCCAGGC AGCCAATCAG 
ACTCTCAGTC CTCAGATGTG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503441.1
CDS	83..1129
Translation	MQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINVLRKEKDDKWKRGNEPAPEKKMEPVVFEKVKMPQKKEDAQLPRKSSPKSAAPVMDLLGLDAPVACSIANSKTSNALEKDLDLLASVPSPSSVSRKAVGSMPTAGSAGSVPENLNLFPEPGSKSEETGKKQLSKDSILSLYGSQTPQMPAQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGGMVPPPVGMVAQPGASGMLTPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMASMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMGGGNGQAANQTLSPQMWK

Target ORF information:

RefSeq Version	XM_006503378.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ArfGAP 2 (Smap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503378.3

ORF Insert Sequence:

ATGCAAGAGA TGGGGAATGG AAAAGCAAAC CGACTCTATG AAGCCTACCT TCCCGAGACC 
TTTCGGCGAC CTCAGATAGA CCCAGCTGTT GAAGGATTTA TTCGAGATAA ATATGAGAAG 
AAGAAATATA TGGACCGAAG TCTGGACATC AATGTCCTTA GGAAAGAGAA GGATGATAAG 
TGGAAACGAG GGAATGAACC CGCTCCGGAG AAAAAGATGG AACCTGTTGT TTTTGAGAAA 
GTAAAAATGC CACAGAAAAA AGAAGACGCA CAGCTACCTC GGAAAAGCTC TCCGAAATCT 
GCAGCCCCTG TCATGGACTT GTTGGGCCTT GATGCTCCTG TGGCCTGCTC AATTGCAAAC 
AGTAAGACCA GCAATGCCCT AGAAAAGGAT CTAGATCTTT TGGCCTCTGT TCCGTCCCCT 
TCTTCAGTTT CCAGAAAGGC TGTAGGTTCC ATGCCAACTG CAGGGAGTGC TGGTTCTGTC 
CCTGAAAACC TGAACCTATT TCCAGAGCCA GGGAGCAAAT CAGAAGAAAC AGGCAAGAAA 
CAGCTCTCCA AGGACTCCAT CCTATCACTG TATGGATCCC AGACGCCTCA AATGCCTGCC 
CAAGCAATGT TCATGGCTCC TGCTCAGATG GCATACCCCA CAGCTTACCC CAGTTTCCCC 
GGGGTTACAC CACCTAACAG CATCATGGGG GGCATGGTGC CTCCGCCAGT AGGCATGGTA 
GCTCAACCGG GAGCCTCTGG AATGCTAACC CCTATGGCCA TGCCTGCAGG CTATATGGGG 
GGCATGCAGG CGTCCATGAT GGGTGTGCCG AACGGGATGA TGACCACTCA GCAGGCCGGC 
TACATGGCAA GCATGGCAGC TATGCCGCAG ACTGTGTATG GAGTTCAGCC AGCTCAGCAA 
CTGCAATGGA ACCTTACTCA GATGACCCAG CAAATGGCTG GGATGAACTT CTACGGAGCC 
AACGGCATGA TGAACTATGG ACAGTCAATG GGCGGTGGAA ATGGCCAGGC AGCCAATCAG 
ACTCTCAGTC CTCAGATGTG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smap2 ( XM_006503379.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503442.1 Mus musculus small ArfGAP 2 (Smap2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu33801
Clone ID Related Accession (Same CDS sequence)	XM_006503379.3 , XM_006503379.4
Accession Version	XM_006503379.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	stromal membrane-associated protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503379.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTACT GTGCTCTATT GAACCCTGGC AATTGGCAAA AGTGGCACTG CTTTGTAAAT 
ATCTTGTATA AAAGTATTGA CTTTGACTGG TTTATCAATA TTGCTCTTCC TCCATTCCCT 
CAGCCCGGGC TGACAGACAC AGAGCAAGCC CATTTGAAGA AAGAGAAGGA TGATAAGTGG 
AAACGAGGGA ATGAACCCGC TCCGGAGAAA AAGATGGAAC CTGTTGTTTT TGAGAAAGTA 
AAAATGCCAC AGAAAAAAGA AGACGCACAG CTACCTCGGA AAAGCTCTCC GAAATCTGCA 
GCCCCTGTCA TGGACTTGTT GGGCCTTGAT GCTCCTGTGG CCTGCTCAAT TGCAAACAGT 
AAGACCAGCA ATGCCCTAGA AAAGGATCTA GATCTTTTGG CCTCTGTTCC GTCCCCTTCT 
TCAGTTTCCA GAAAGGCTGT AGGTTCCATG CCAACTGCAG GGAGTGCTGG TTCTGTCCCT 
GAAAACCTGA ACCTATTTCC AGAGCCAGGG AGCAAATCAG AAGAAACAGG CAAGAAACAG 
CTCTCCAAGG ACTCCATCCT ATCACTGTAT GGATCCCAGA CGCCTCAAAT GCCTGCCCAA 
GCAATGTTCA TGGCTCCTGC TCAGATGGCA TACCCCACAG CTTACCCCAG TTTCCCCGGG 
GTTACACCAC CTAACAGCAT CATGGGGGGC ATGGTGCCTC CGCCAGTAGG CATGGTAGCT 
CAACCGGGAG CCTCTGGAAT GCTAACCCCT ATGGCCATGC CTGCAGGCTA TATGGGGGGC 
ATGCAGGCGT CCATGATGGG TGTGCCGAAC GGGATGATGA CCACTCAGCA GGCCGGCTAC 
ATGGCAAGCA TGGCAGCTAT GCCGCAGACT GTGTATGGAG TTCAGCCAGC TCAGCAACTG 
CAATGGAACC TTACTCAGAT GACCCAGCAA ATGGCTGGGA TGAACTTCTA CGGAGCCAAC 
GGCATGATGA ACTATGGACA GTCAATGGGC GGTGGAAATG GCCAGGCAGC CAATCAGACT 
CTCAGTCCTC AGATGTGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503442.1
CDS	835..1878
Translation	MAYCALLNPGNWQKWHCFVNILYKSIDFDWFINIALPPFPQPGLTDTEQAHLKKEKDDKWKRGNEPAPEKKMEPVVFEKVKMPQKKEDAQLPRKSSPKSAAPVMDLLGLDAPVACSIANSKTSNALEKDLDLLASVPSPSSVSRKAVGSMPTAGSAGSVPENLNLFPEPGSKSEETGKKQLSKDSILSLYGSQTPQMPAQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGGMVPPPVGMVAQPGASGMLTPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMASMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMGGGNGQAANQTLSPQMWK

Target ORF information:

RefSeq Version	XM_006503379.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ArfGAP 2 (Smap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503379.4

ORF Insert Sequence:

ATGGCTTACT GTGCTCTATT GAACCCTGGC AATTGGCAAA AGTGGCACTG CTTTGTAAAT 
ATCTTGTATA AAAGTATTGA CTTTGACTGG TTTATCAATA TTGCTCTTCC TCCATTCCCT 
CAGCCCGGGC TGACAGACAC AGAGCAAGCC CATTTGAAGA AAGAGAAGGA TGATAAGTGG 
AAACGAGGGA ATGAACCCGC TCCGGAGAAA AAGATGGAAC CTGTTGTTTT TGAGAAAGTA 
AAAATGCCAC AGAAAAAAGA AGACGCACAG CTACCTCGGA AAAGCTCTCC GAAATCTGCA 
GCCCCTGTCA TGGACTTGTT GGGCCTTGAT GCTCCTGTGG CCTGCTCAAT TGCAAACAGT 
AAGACCAGCA ATGCCCTAGA AAAGGATCTA GATCTTTTGG CCTCTGTTCC GTCCCCTTCT 
TCAGTTTCCA GAAAGGCTGT AGGTTCCATG CCAACTGCAG GGAGTGCTGG TTCTGTCCCT 
GAAAACCTGA ACCTATTTCC AGAGCCAGGG AGCAAATCAG AAGAAACAGG CAAGAAACAG 
CTCTCCAAGG ACTCCATCCT ATCACTGTAT GGATCCCAGA CGCCTCAAAT GCCTGCCCAA 
GCAATGTTCA TGGCTCCTGC TCAGATGGCA TACCCCACAG CTTACCCCAG TTTCCCCGGG 
GTTACACCAC CTAACAGCAT CATGGGGGGC ATGGTGCCTC CGCCAGTAGG CATGGTAGCT 
CAACCGGGAG CCTCTGGAAT GCTAACCCCT ATGGCCATGC CTGCAGGCTA TATGGGGGGC 
ATGCAGGCGT CCATGATGGG TGTGCCGAAC GGGATGATGA CCACTCAGCA GGCCGGCTAC 
ATGGCAAGCA TGGCAGCTAT GCCGCAGACT GTGTATGGAG TTCAGCCAGC TCAGCAACTG 
CAATGGAACC TTACTCAGAT GACCCAGCAA ATGGCTGGGA TGAACTTCTA CGGAGCCAAC 
GGCATGATGA ACTATGGACA GTCAATGGGC GGTGGAAATG GCCAGGCAGC CAATCAGACT 
CTCAGTCCTC AGATGTGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality.
FEBS letters589(19 Pt B)2754-62(2015 Sep)
Sumiyoshi M,Masuda N,Tanuma N,Ogoh H,Imai E,Otsuka M,Hayakawa N,Ohno K,Matsui Y,Hara K,Gotoh R,Suzuki M,Rai S,Tanaka H,Matsumura I,Shima H,Watanabe T

The Arf GAP SMAP2 is necessary for organized vesicle budding from the trans-Golgi network and subsequent acrosome formation in spermiogenesis.
Molecular biology of the cell24(17)2633-44(2013 Sep)
Funaki T,Kon S,Tanabe K,Natsume W,Sato S,Shimizu T,Yoshida N,Wong WF,Ogura A,Ogawa T,Inoue K,Ogonuki N,Miki H,Mochida K,Endoh K,Yomogida K,Fukumoto M,Horai R,Iwakura Y,Ito C,Toshimori K,Watanabe T,Satake M

Genomic strategy identifies a missense mutation in WD-repeat domain 65 (WDR65) in an individual with Van der Woude syndrome.
American journal of medical genetics. Part A155A(6)1314-21(2011 Jun)
Rorick NK,Kinoshita A,Weirather JL,Peyrard-Janvid M,de Lima RL,Dunnwald M,Shanske AL,Moretti-Ferreira D,Koillinen H,Kere J,Mansilla MA,Murray JC,Goudy SL,Schutte BC

Localization of SMAP2 to the TGN and its function in the regulation of TGN protein transport.
Cell structure and function36(1)83-95(2011)
Funaki T,Kon S,Ronn RE,Henmi Y,Kobayashi Y,Watanabe T,Nakayama K,Tanabe K,Satake M

SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network.
Molecular biology of the cell17(6)2592-603(2006 Jun)
Natsume W,Tanabe K,Kon S,Yoshida N,Watanabe T,Torii T,Satake M

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Smap2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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