gRNAs
The following Tmem52 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmem52 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu19297 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001253856.1 | |
| Accession Version | NM_001253856.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | transmembrane protein 52 isoform 2 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL670227.7. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK009779.1, BY709403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCCCCGG GGCCGTCAGC CACACAGGGG ATCCTGCTGC TGCTGCCTCT CCTGCCTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001240785.1 |
| CDS | 32..622 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001253856.1 |
1 | ATGGCCCCGG GGCCGTCAGC CACACAGGGG ATCCTGCTGC TGCTGCCTCT CCTGCCTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu19399 | |
| Clone ID Related Accession (Same CDS sequence) | NM_027161.2 | |
| Accession Version | NM_027161.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | transmembrane protein 52 isoform 1 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL670227.7 and BC144992.1. On Dec 8, 2011 this sequence version replaced NM_027161.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC144992.1, W41266.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCCCCGG GGCCGTCAGC CACACAGGGG ATCCTGCTGC TGCTGCCTCT CCTGCCTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_081437.1 |
| CDS | 32..640 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_027161.2 |
1 | ATGGCCCCGG GGCCGTCAGC CACACAGGGG ATCCTGCTGC TGCTGCCTCT CCTGCCTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu19470 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001253858.1 | |
| Accession Version | NM_001253858.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | transmembrane protein 52 isoform 3 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL670227.7. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BY343314.1, BY341932.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCCCCGG GGCCGTCAGC CACACAGGGG ATCCTGCTGC TGCTGCCTCT CCTGCCTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001240787.1 |
| CDS | 32..580 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001253858.1 |
1 | ATGGCCCCGG GGCCGTCAGC CACACAGGGG ATCCTGCTGC TGCTGCCTCT CCTGCCTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A conditional knockout resource for the genome-wide study of mouse gene function. |
