Mtfmt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mtfmt
Entrez Gene ID	69606
Full Name	mitochondrial methionyl-tRNA formyltransferase
Synonyms	2310020P08Rik
General protein information	Preferred Names mitochondrial methionyl-tRNA formyltransferase Names methionyl-tRNA formyltransferase, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_027134.3	NP_081410.2	methionyl-tRNA formyltransferase, mitochondrial

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mtfmt	NP_081410.2
Drosophila melanogaster (fruit fly)	CG1750	NP_651857.2
Arabidopsis thaliana (thale cress)	pde194	NP_176825.1
Danio rerio (zebrafish)	mtfmt	NP_001071010.2
Bos taurus (cattle)	MTFMT	NP_001159995.1
Gallus gallus (chicken)	MTFMT	XP_413901.3
Oryza sativa (rice)	Os01g0687500	NP_001043913.1
Homo sapiens (human)	MTFMT	NP_640335.2
Pan troglodytes (chimpanzee)	MTFMT	XP_510478.2
Canis lupus familiaris (dog)	MTFMT	XP_853405.2
Rattus norvegicus (Norway rat)	Mtfmt	NP_001009697.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000398	XP_310702.5
Xenopus tropicalis (tropical clawed frog)	mtfmt	XP_002938392.2

Gene Ontology
Bibliography

Related articles in PubMed

Cre recombinase activity is inhibited in vivo but not ex vivo by a mutation in the asymmetric spacer region of the distal loxP site.
Arguello T, Moraes CT
Genesis (New York, N.Y. : 2000)53(11)695-700(2015 Nov)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mtfmt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mtfmt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13202	NM_027134.3 Latest version!	Mus musculus mitochondrial methionyl-tRNA formyltransferase (Mtfmt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mtfmt ( NM_027134.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081410.2 Mus musculus mitochondrial methionyl-tRNA formyltransferase (Mtfmt), mRNA.

Price & Availability↑

CloneID	OMu13202
Clone ID Related Accession (Same CDS sequence)	NM_027134.3
Accession Version	NM_027134.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	methionyl-tRNA formyltransferase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK009430.1 and AK133492.1. On Apr 25, 2009 this sequence version replaced NM_027134.2. ##Evidence-Data-START## Transcript exon combination :: AK133492.1, SRR1660819.74034.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCTCCTTC CGCGGTGCTG CTGGGGTCGG TGGCTGATGG GAAGGAGGCC ACGCTGTTCC 
TGTCAAGCAC CTGCCGGGTT CGACGGGAAG GACGGCCGAG GTTCCCGAGT CCGCGAGAAG 
CCGCCTTGGC GGGTGCTGTT CCTCGGCACC GACCACTTTG CCCGGGAAAC GCTGCGGGCG 
CTGCACGCCG CCAGAGACGG CAAAGAGGAA AAGTTAATTG AGAAACTGGA GGTTGTCACA 
GTGCCTTCGC TATCCCCCAA AGGACTACCT GTGAAGCAAT ATGCTATTCA GTCTCAGCTC 
CCGGTGTACG AGTGGCCCGA CGTGGGATCT GGAGAATATG ATGTTGGGGT AGTGGCTTCC 
TTTGGCCGGC TTTTGAGTGA GGCTCTTATT CTTAAATTTC CCTACGGCAT ACTGAATGTG 
CACCCCAGTT GCCTCCCGAG GTGGCGTGGT CCCGCTCCGA TAATCCACAC GGTGCTTCAT 
GGAGACACTG TCACTGGCGT AACGATTATG CAAATTAGAC CCAAAAGATT TGATATAGGC 
CCAATTCTCC AGCAGGAAAC CATCCCTGTG CCTCCCAAGA GCACATCAAA GGAATTGGAA 
GCAGTGTTGT CAAAACTTGG TGCCAACATG CTTATCTCAG TTTTGAAAAA TCTGCCCGAA 
AGTCTGAACA ATGGAAGGCC GCAGCCAGCC GAGGGAGTGA CATATGCCCC CAAGGTCTCT 
GCTGGCACCA GCTGTGTCAA GTGGGAGGAG CAAACCTCAG AGCAAGTACT CAGACTTCAC 
CTTGCCATTG GGGATATAGT CCCACTGCAG ACGCTCTGGA TGGAGAACAC CGTTAAACTT 
CTGGATCTCG TAGAAGTTAA CAATTCCATC CTTGCAGATC CGAAATTAAC AGGACAAACT 
GTAACTCCGG GGTTCGTGGT GTACCACAGA CCATCGCAAA TGCTGCTGGT TCGCTGTAAG 
GATGGCTGGA TCGGTGTTCG GTCAGTAATG CTTAAGAAAA CACTAACAGC TACTGATTTC 
TACAATGGAT ATTTGCATGC GTGGTACCAG AAAAATTCCC ACGCTCACCC AAGCCAGTGC 
AGATTTCAGA CTCTCAGACT TCCAACAAAG ATGCAGCAGA AAACAAAGTT GTTGCTATGC 
AACAGTGCAT TAAGTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081410.2
CDS	33..1193
Translation	MLLPRCCWGRWLMGRRPRCSCQAPAGFDGKDGRGSRVREKPPWRVLFLGTDHFARETLRALHAARDGKEEKLIEKLEVVTVPSLSPKGLPVKQYAIQSQLPVYEWPDVGSGEYDVGVVASFGRLLSEALILKFPYGILNVHPSCLPRWRGPAPIIHTVLHGDTVTGVTIMQIRPKRFDIGPILQQETIPVPPKSTSKELEAVLSKLGANMLISVLKNLPESLNNGRPQPAEGVTYAPKVSAGTSCVKWEEQTSEQVLRLHLAIGDIVPLQTLWMENTVKLLDLVEVNNSILADPKLTGQTVTPGFVVYHRPSQMLLVRCKDGWIGVRSVMLKKTLTATDFYNGYLHAWYQKNSHAHPSQCRFQTLRLPTKMQQKTKLLLCNSALSS

Target ORF information:

RefSeq Version	NM_027134.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial methionyl-tRNA formyltransferase (Mtfmt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027134.3

ORF Insert Sequence:

ATGCTCCTTC CGCGGTGCTG CTGGGGTCGG TGGCTGATGG GAAGGAGGCC ACGCTGTTCC 
TGTCAAGCAC CTGCCGGGTT CGACGGGAAG GACGGCCGAG GTTCCCGAGT CCGCGAGAAG 
CCGCCTTGGC GGGTGCTGTT CCTCGGCACC GACCACTTTG CCCGGGAAAC GCTGCGGGCG 
CTGCACGCCG CCAGAGACGG CAAAGAGGAA AAGTTAATTG AGAAACTGGA GGTTGTCACA 
GTGCCTTCGC TATCCCCCAA AGGACTACCT GTGAAGCAAT ATGCTATTCA GTCTCAGCTC 
CCGGTGTACG AGTGGCCCGA CGTGGGATCT GGAGAATATG ATGTTGGGGT AGTGGCTTCC 
TTTGGCCGGC TTTTGAGTGA GGCTCTTATT CTTAAATTTC CCTACGGCAT ACTGAATGTG 
CACCCCAGTT GCCTCCCGAG GTGGCGTGGT CCCGCTCCGA TAATCCACAC GGTGCTTCAT 
GGAGACACTG TCACTGGCGT AACGATTATG CAAATTAGAC CCAAAAGATT TGATATAGGC 
CCAATTCTCC AGCAGGAAAC CATCCCTGTG CCTCCCAAGA GCACATCAAA GGAATTGGAA 
GCAGTGTTGT CAAAACTTGG TGCCAACATG CTTATCTCAG TTTTGAAAAA TCTGCCCGAA 
AGTCTGAACA ATGGAAGGCC GCAGCCAGCC GAGGGAGTGA CATATGCCCC CAAGGTCTCT 
GCTGGCACCA GCTGTGTCAA GTGGGAGGAG CAAACCTCAG AGCAAGTACT CAGACTTCAC 
CTTGCCATTG GGGATATAGT CCCACTGCAG ACGCTCTGGA TGGAGAACAC CGTTAAACTT 
CTGGATCTCG TAGAAGTTAA CAATTCCATC CTTGCAGATC CGAAATTAAC AGGACAAACT 
GTAACTCCGG GGTTCGTGGT GTACCACAGA CCATCGCAAA TGCTGCTGGT TCGCTGTAAG 
GATGGCTGGA TCGGTGTTCG GTCAGTAATG CTTAAGAAAA CACTAACAGC TACTGATTTC 
TACAATGGAT ATTTGCATGC GTGGTACCAG AAAAATTCCC ACGCTCACCC AAGCCAGTGC 
AGATTTCAGA CTCTCAGACT TCCAACAAAG ATGCAGCAGA AAACAAAGTT GTTGCTATGC 
AACAGTGCAT TAAGTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cre recombinase activity is inhibited in vivo but not ex vivo by a mutation in the asymmetric spacer region of the distal loxP site.
Genesis (New York, N.Y. : 2000)53(11)695-700(2015 Nov)
Arguello T,Moraes CT

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

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Mtfmt cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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